pycom01g15680

Fizzy-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009046
Physical Location & Seq
Reverse (-)
15452726 .. 15455859
3134 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1800 bp
ATGCACACTGCACATATTACAATTTATTCACAGGGAAAAAAAAAAAAAAAAATAAGGAAGAGAAAAACCCAGCACCGCTCCTATAAACCCACCGTAATTCGACCGTTACAAGTCTTCCGCCACACTGAACTAAGAGAGAGAAACTCTAGAGAGAGAAAGAGAGAGAGAGGCCTTTCCTTTCATTTCTGTTCCTTTGGCCTCCTCCTTCTCTCTTCAAAAACCCCATTAATTATACCAATTAGCTATATGATATCTTCACATTCTTCAGACCCAACAACCCAGCAACTCCTCCTCCAAGCAACCAAGAACCGCATCCAACAGAACCCAACAATGGAGTCACCTCAAACCAGGAAATCGGGGCTCAACCTCCCCTCCGGGATGTCCGAGACATTGCTCCGCCTCGATTCCGTTTCGGGTTCCTTTCGTGCGGTTTCGAGCCTCTCAGCGTCGCCGGGTTCTTGTCGGGCGATTTCGAACCTGTCCTCGCCGTCGAAGTCCTCGACTTGCAGCGACAGATTCATCCCCTGCAGATCCTCGTCGCGGCTTCACACGTTCGGGCTCAACGAGAAGGCCTCGCCGGTGAAAGAAGGAGGCAACGAGGCGTATGCCAGGTTGTTGAAATCGGAGCTTTTCGGGTCTGATTTCGGGTCTTTTTCTCCTGCAGGTGGCGGAGGTGGTGGTGCAGCGTCGCCCATGAGTCCCAGCAAGAACATGTTGCGGTTCAAGACGGAGAACTCGGGCCCGAACTCGCCTTACTCGCCGTCCCTTCTGAGAAATGACAGTGGGTTTTCCAGCGAGACGACGCCTCCCAAGGCTCCTCGCAAGGTTCCCAAGACACCCCATAAGGTTTTGGACGCTCCATCACTACAAGATGACTTTTACTTGAACCTTGTGGACTGGTCCTCCCAAAATGTCCTTGCAGTTGGACTGGGCACCAGTGTTTATCTCTGGAGTGCATCAAACAGCAAGGTGACAAAACTGTGTGACTTGGGGCCTAATGACGGCGTGTGCTCTGTCCAATGGACACGGGAGGGTTCATACTTGTCGATTGGCACAAGTCTTGGTCGAGTTCAGGTTTGGGATGGGACGCAGTGCAAGAGGGTCCGAACAATGGGCGGGCATCAAACAAGGACTGGAGTCTTGGCGTGGAACTCGCGCATATTAGCTTCAGGGAGCCGAGATCGGAATATACTTCAACACGACCTTCGAGTCCCAGACAACTATATCAGCAAGCTTGTTGGCCACAAGTCTGAGGTATGTGGGCTGAAATGGTCCAATGATGACAGGGAACTTGCATCCGGTGGCAATGATAACCAGCTCTTGGTCTGGAACCAGCAGTCTCAGCAACCGGCTTTGAGACTGACAGAGCACACAGCTGCAGTCAAGGCCATTGCTTGGTCACCCCACCAGAGTGGCCTTCTTGCATCTGGAGGCGGAACTGCTGACAGATGTATTCGGTTCTGGAACACTACAAACGGCCACCAATTGAACAGCATCGACACAGGAAGCCAGGTCTGCAATCTTGCATGGAGTAAGAATGTCAATGAGATAGTCAGCACTCATGGTTATTCCCAGAATCAAATTATGGTGTGGAAGTATCCATCCATGGGAAAGGTTGCCAATCTAACCGGCCATAGCATGCGAGTTTTGTACCTTGCAATGTCACCGGATGGTCAGACAATAGTCACTGGTGCTGGGGATGAGACTCTAAGATTCTGGAACGTCTTTCCATCTGTGAAGACACATACGTCAGTGAAGGATACGGGTCTTTGGTCACTAGGAAGAACTCAGATTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04120 ko04914 map04110 map04111 map04120 map04914
Pfam Domains
Protein Families
WD40

Protein Analysis

600

Amino Acids

65.79

Weight (kDa)

9.98

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 284 - 573 2.6e-128 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 299 - 397 1e-07 THOC3 beta-propeller domain
Beta-prop_WDR19_1st PF23389 299 - 397 1e-11 WDR19 first beta-propeller
Beta-prop_EML_2 PF23414 302 - 471 6.2e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 305 - 401 2e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 310 - 403 8.2e-08 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 330 - 574 5.2e-14 MABP1/WDR62 second WD40 domain
WDR55 PF24796 334 - 578 2.4e-10 WDR55
WD40_Prp19 PF24814 385 - 575 2.9e-20 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 387 - 519 2.4e-17 WDR5 beta-propeller domain
WD40 PF00400 409 - 444 3.1e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 410 - 510 1.4e-08 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 410 - 519 7.5e-17 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 411 - 581 1.5e-11 G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1 PF24105 411 - 499 4.4e-11 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 412 - 520 5.6e-06 EIF3I
WD40_WDHD1_1st PF24817 415 - 474 2e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 415 - 520 5.5e-22 THOC3 beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 452 - 581 5.8e-06 MABP1/WDR62 first WD40 domain
Beta-prop_EML PF23409 455 - 576 7.1e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 456 - 576 2.8e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 463 - 583 7.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 481 - 576 1.1e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 503 - 577 4.1e-08 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 524 - 576 5.9e-08 THOC3 beta-propeller domain
WD40 PF00400 539 - 574 2.8e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 653
AasI GACNNNNNNGTC 2 cut(s) 1208, 1747
Acc36I ACCTGC 1 cut(s) 653
AccB1I GGYRCC 1 cut(s) 932
AccB7I CCANNNNNTGG 2 cut(s) 1321, 1395
AccBSI CCGCTC 1 cut(s) 78
AccII CGCG 2 cut(s) 541, 1156
AclWI GGATC 1 cut(s) 525
AcoI YGGCCR 3 cut(s) 1240, 1477, 1630
AcuI CTGAAG 2 cut(s) 249, 1152
AcyI GRCGYC 1 cut(s) 803
AfaI GTAC 1 cut(s) 1652
AfiI CCNNNNNNNGG 8 cut(s) 331, 540, 665, 1001, 1111, 1321, 1395, 1607
AflIII ACRYGT 2 cut(s) 549, 711
AgsI TTSAA 6 cut(s) 216, 619, 724, 886, 1196, 1489
AjnI CCWGG 3 cut(s) 347, 608, 1509
AleI CACNNNNGTG 1 cut(s) 1410
AluBI AGCT 6 cut(s) 243, 628, 1166, 1234, 1318, 1376
AluI AGCT 6 cut(s) 243, 628, 1166, 1234, 1318, 1376
Alw21I GWGCWC 2 cut(s) 1013, 1371
Alw26I GTCTC 5 cut(s) 380, 791, 1344, 1351, 1697
AlwI GGATC 1 cut(s) 525
Ama87I CYCGRG 1 cut(s) 736
ApaI GGGCCC 1 cut(s) 743
ApeKI GCWGC 3 cut(s) 507, 683, 1376
AseI ATTAAT 1 cut(s) 227
AspLEI GCGC 1 cut(s) 1158
AspS9I GGNCC 6 cut(s) 739, 740, 900, 992, 1102, 1272
AsuC2I CCSGG 2 cut(s) 376, 453
AsuHPI GGTGA 5 cut(s) 330, 592, 982, 1392, 1656
AsuII TTCGAA 1 cut(s) 473
AvaI CYCGRG 1 cut(s) 736
AvaII GGWCC 3 cut(s) 900, 1102, 1272
BaeGI GKGCMC 2 cut(s) 743, 935
BalI TGGCCA 1 cut(s) 1242
BanI GGYRCC 1 cut(s) 932
BanII GRGCYC 3 cut(s) 363, 561, 743
BbsI GAAGAC 2 cut(s) 106, 1745
Bbv12I GWGCWC 2 cut(s) 1013, 1371
BbvI GCAGC 3 cut(s) 519, 695, 1363
BccI CCATC 5 cut(s) 868, 1076, 1609, 1664, 1738
BceAI ACGGC 4 cut(s) 472, 745, 1018, 1492
BcgI CGANNNNNNTGC 2 cut(s) 587, 621
BciT130I CCWGG 3 cut(s) 349, 610, 1511
BciVI GTATCC 2 cut(s) 1608, 1753
BcnI CCSGG 2 cut(s) 376, 453
BcoDI GTCTC 5 cut(s) 380, 791, 1344, 1351, 1697
BfaI CTAG 2 cut(s) 147, 1778
BfmI CTRYAG 3 cut(s) 526, 660, 1377
BfuAI ACCTGC 1 cut(s) 653
BfuI GTATCC 2 cut(s) 1608, 1753
BisI GCNGC 4 cut(s) 508, 542, 684, 1377
BlsI GCNGC 4 cut(s) 509, 543, 685, 1378
Bme1390I CCNGG 5 cut(s) 349, 376, 453, 610, 1511
Bme18I GGWCC 3 cut(s) 900, 1102, 1272
BmeT110I CYCGRG 1 cut(s) 736
BmgT120I GGNCC 6 cut(s) 739, 740, 900, 992, 1102, 1272
BmiI GGNNCC 9 cut(s) 418, 741, 816, 828, 934, 993, 1103, 1175, 1331
BmrFI CCNGG 5 cut(s) 349, 376, 453, 610, 1511
BmrI ACTGGG 1 cut(s) 938
BmsI GCATC 6 cut(s) 321, 965, 1129, 1304, 1433, 1503
BmuI ACTGGG 1 cut(s) 938
BoxI GACNNNNGTC 2 cut(s) 1136, 1682
BpiI GAAGAC 2 cut(s) 106, 1745
BplI GAGNNNNNCTC 4 cut(s) 128, 160, 557, 589
BpmI CTGGAG 3 cut(s) 970, 1155, 1449
Bpu14I TTCGAA 1 cut(s) 473
BpuMI CCSGG 2 cut(s) 376, 453
BsaHI GRCGYC 1 cut(s) 803
BsaJI CCNNGG 2 cut(s) 810, 1605
BsaWI WCCGGW 2 cut(s) 1298, 1666
BsaXI ACNNNNNCTCC 8 cut(s) 276, 306, 356, 386, 790, 820, 887, 917
Bsc4I CCNNNNNNNGG 8 cut(s) 331, 540, 665, 1001, 1111, 1321, 1395, 1607
Bse118I RCCGGY 3 cut(s) 577, 1348, 1628
Bse1I ACTGG 5 cut(s) 902, 933, 936, 1138, 1693
Bse3DI GCAATG 4 cut(s) 389, 1312, 1389, 1665
BseBI CCWGG 3 cut(s) 349, 610, 1511
BseDI CCNNGG 2 cut(s) 810, 1605
BseGI GGATG 8 cut(s) 312, 384, 519, 1087, 1295, 1601, 1675, 1705
BseLI CCNNNNNNNGG 8 cut(s) 331, 540, 665, 1001, 1111, 1321, 1395, 1607
BseMI GCAATG 4 cut(s) 389, 1312, 1389, 1665
BseMII CTCAG 4 cut(s) 456, 761, 1242, 1355
BseNI ACTGG 5 cut(s) 902, 933, 936, 1138, 1693
BseRI GAGGAG 4 cut(s) 191, 278, 281, 807
BseSI GKGCMC 2 cut(s) 743, 935
BseXI GCAGC 3 cut(s) 519, 695, 1363
BseYI CCCAGC 4 cut(s) 69, 279, 701, 1694
BsgI GTGCAG 1 cut(s) 702
Bsh1236I CGCG 2 cut(s) 541, 1156
Bsh1285I CGRYCG 1 cut(s) 104
BshNI GGYRCC 1 cut(s) 932
BsiEI CGRYCG 1 cut(s) 104
BsiHKAI GWGCWC 2 cut(s) 1013, 1371
BsiHKCI CYCGRG 1 cut(s) 736
BsiSI CCGG 7 cut(s) 375, 452, 578, 1299, 1349, 1629, 1667
BslFI GGGAC 4 cut(s) 684, 748, 1099, 1196
BslI CCNNNNNNNGG 8 cut(s) 331, 540, 665, 1001, 1111, 1321, 1395, 1607
BsmAI GTCTC 5 cut(s) 380, 791, 1344, 1351, 1697
BsmBI CGTCTC 1 cut(s) 791
BsmFI GGGAC 4 cut(s) 684, 748, 1099, 1196
BsoBI CYCGRG 1 cut(s) 736
Bsp119I TTCGAA 1 cut(s) 473
Bsp120I GGGCCC 1 cut(s) 739
Bsp1286I GDGCHC 6 cut(s) 363, 561, 743, 935, 1013, 1371
Bsp143I GATC 2 cut(s) 530, 1180
Bsp19I CCATGG 1 cut(s) 1605
BspCNI CTCAG 4 cut(s) 455, 762, 1243, 1354
BspFNI CGCG 2 cut(s) 541, 1156
BspLI GGNNCC 9 cut(s) 418, 741, 816, 828, 934, 993, 1103, 1175, 1331
BspMAI CTGCAG 3 cut(s) 530, 664, 1381
BspMI ACCTGC 1 cut(s) 653
BspPI GGATC 1 cut(s) 525
BspT104I TTCGAA 1 cut(s) 473
BspT107I GGYRCC 1 cut(s) 932
BsrBI CCGCTC 1 cut(s) 78
BsrDI GCAATG 4 cut(s) 389, 1312, 1389, 1665
BsrFI RCCGGY 3 cut(s) 577, 1348, 1628
BsrI ACTGG 5 cut(s) 902, 933, 936, 1138, 1693
BssAI RCCGGY 3 cut(s) 577, 1348, 1628
BssECI CCNNGG 2 cut(s) 810, 1605
BssMI GATC 2 cut(s) 530, 1180
BssNI GRCGYC 1 cut(s) 803
BssT1I CCWWGG 2 cut(s) 810, 1605
Bst2UI CCWGG 3 cut(s) 349, 610, 1511
Bst4CI ACNGT 4 cut(s) 94, 105, 782, 981
Bst6I CTCTTC 2 cut(s) 53, 217
BstACI GRCGYC 1 cut(s) 803
BstBI TTCGAA 1 cut(s) 473
BstC8I GCNNGC 3 cut(s) 1118, 1232, 1640
BstDEI CTNAG 7 cut(s) 131, 442, 770, 1251, 1341, 1709, 1788
BstDSI CCRYGG 1 cut(s) 1605
BstEII GGTNACC 1 cut(s) 1398
BstF5I GGATG 8 cut(s) 312, 384, 519, 1087, 1295, 1601, 1675, 1705
BstFNI CGCG 2 cut(s) 541, 1156
BstHHI GCGC 1 cut(s) 1158
BstKTI GATC 2 cut(s) 533, 1183
BstMAI GTCTC 5 cut(s) 380, 791, 1344, 1351, 1697
BstMBI GATC 2 cut(s) 530, 1180
BstMCI CGRYCG 1 cut(s) 104
BstMWI GCNNNNNNNGC 4 cut(s) 757, 1342, 1385, 1515
BstNI CCWGG 3 cut(s) 349, 610, 1511
BstNSI RCATGY 2 cut(s) 715, 1642
BstPAI GACNNNNGTC 2 cut(s) 1136, 1682
BstPI GGTNACC 1 cut(s) 1398
BstSCI CCNGG 5 cut(s) 347, 374, 451, 608, 1509
BstSFI CTRYAG 3 cut(s) 526, 660, 1377
BstSLI GKGCMC 2 cut(s) 743, 935
BstUI CGCG 2 cut(s) 541, 1156
BstV1I GCAGC 3 cut(s) 519, 695, 1363
BstV2I GAAGAC 2 cut(s) 106, 1745
BstX2I RGATCY 1 cut(s) 530
BstXI CCANNNNNNTGG 1 cut(s) 1412
BstYI RGATCY 1 cut(s) 530
BsuI GTATCC 2 cut(s) 1608, 1753
BtgI CCRYGG 1 cut(s) 1605
BtsCI GGATG 8 cut(s) 312, 384, 519, 1087, 1295, 1601, 1675, 1705
BtsI GCAGTG 2 cut(s) 6, 1097
BtsIMutI CAGTG 7 cut(s) 6, 123, 787, 943, 1097, 1686, 1758
BveI ACCTGC 1 cut(s) 653
Cac8I GCNNGC 3 cut(s) 1118, 1232, 1640
CfoI GCGC 1 cut(s) 1158
Cfr10I RCCGGY 3 cut(s) 577, 1348, 1628
Cfr13I GGNCC 6 cut(s) 739, 740, 900, 992, 1102, 1272
CseI GACGC 5 cut(s) 435, 675, 811, 863, 1096
Csp6I GTAC 1 cut(s) 1651
CviAII CATG 6 cut(s) 694, 712, 1527, 1562, 1606, 1639
CviQI GTAC 1 cut(s) 1651
DdeI CTNAG 7 cut(s) 131, 442, 770, 1251, 1341, 1709, 1788
DpnI GATC 2 cut(s) 532, 1182
DpnII GATC 2 cut(s) 530, 1180
DrdI GACNNNNNNGTC 2 cut(s) 1208, 1747
DseDI GACNNNNNNGTC 2 cut(s) 1208, 1747
EaeI YGGCCR 3 cut(s) 1240, 1477, 1630
Eam1104I CTCTTC 2 cut(s) 53, 217
EarI CTCTTC 2 cut(s) 53, 217
EciI GGCGGA 4 cut(s) 107, 386, 684, 1449
Eco130I CCWWGG 2 cut(s) 810, 1605
Eco147I AGGCCT 2 cut(s) 171, 572
Eco24I GRGCYC 3 cut(s) 363, 561, 743
Eco32I GATATC 1 cut(s) 252
Eco47I GGWCC 3 cut(s) 900, 1102, 1272
Eco57I CTGAAG 2 cut(s) 249, 1152
Eco88I CYCGRG 1 cut(s) 736
Eco91I GGTNACC 1 cut(s) 1398
EcoO109I RGGNCCY 1 cut(s) 992
EcoO65I GGTNACC 1 cut(s) 1398
EcoRII CCWGG 3 cut(s) 347, 608, 1509
EcoRV GATATC 1 cut(s) 252
EcoT14I CCWWGG 2 cut(s) 810, 1605
EcoT38I GRGCYC 3 cut(s) 363, 561, 743
ErhI CCWWGG 2 cut(s) 810, 1605
Esp3I CGTCTC 1 cut(s) 791
FaeI CATG 6 cut(s) 697, 715, 1530, 1565, 1609, 1642
FaqI GGGAC 4 cut(s) 684, 748, 1099, 1196
FatI CATG 6 cut(s) 693, 711, 1526, 1561, 1605, 1638
FauI CCCGC 1 cut(s) 1109
Fnu4HI GCNGC 4 cut(s) 508, 542, 684, 1377
FokI GGATG 8 cut(s) 299, 391, 506, 1094, 1282, 1588, 1682, 1712
FriOI GRGCYC 3 cut(s) 363, 561, 743
Fsp4HI GCNGC 4 cut(s) 508, 542, 684, 1377
FspBI CTAG 2 cut(s) 147, 1778
GlaI GCGC 1 cut(s) 1157
GluI GCNGC 4 cut(s) 508, 542, 684, 1377
GsaI CCCAGC 4 cut(s) 73, 283, 705, 1698
GsuI CTGGAG 3 cut(s) 970, 1155, 1449
HapII CCGG 7 cut(s) 375, 452, 578, 1299, 1349, 1629, 1667
HgaI GACGC 5 cut(s) 435, 675, 811, 863, 1096
HhaI GCGC 1 cut(s) 1158
Hin1I GRCGYC 1 cut(s) 803
Hin1II CATG 6 cut(s) 697, 715, 1530, 1565, 1609, 1642
Hin6I GCGC 1 cut(s) 1156
HinP1I GCGC 1 cut(s) 1156
HindIII AAGCTT 1 cut(s) 1232
HpaII CCGG 7 cut(s) 375, 452, 578, 1299, 1349, 1629, 1667
HphI GGTGA 5 cut(s) 330, 592, 982, 1392, 1656
Hpy166II GTNNAC 1 cut(s) 895
Hpy188III TCNNGA 7 cut(s) 147, 724, 949, 1327, 1428, 1462, 1717
Hpy8I GTNNAC 1 cut(s) 895
Hpy99I CGWCG 5 cut(s) 451, 493, 541, 691, 805
HpyAV CCTTC 7 cut(s) 215, 562, 581, 776, 1214, 1427, 1750
HpyCH4III ACNGT 4 cut(s) 94, 105, 782, 981
HpyCH4IV ACGT 3 cut(s) 551, 1722, 1748
HpyF10VI GCNNNNNNNGC 4 cut(s) 757, 1342, 1385, 1515
HpyF3I CTNAG 7 cut(s) 131, 442, 770, 1251, 1341, 1709, 1788
HpySE526I ACGT 3 cut(s) 551, 1722, 1748
Hsp92I GRCGYC 1 cut(s) 803
Hsp92II CATG 6 cut(s) 697, 715, 1530, 1565, 1609, 1642
HspAI GCGC 1 cut(s) 1156
Kzo9I GATC 2 cut(s) 530, 1180
LmnI GCTCC 6 cut(s) 83, 399, 625, 820, 862, 1173
Lsp1109I GCAGC 3 cut(s) 519, 695, 1363
LweI GCATC 6 cut(s) 321, 965, 1129, 1304, 1433, 1503
MaeI CTAG 2 cut(s) 147, 1778
MaeII ACGT 3 cut(s) 551, 1722, 1748
MaeIII GTNAC 8 cut(s) 105, 336, 970, 983, 1398, 1662, 1684, 1773
MalI GATC 2 cut(s) 532, 1182
MbiI CCGCTC 1 cut(s) 78
MboI GATC 2 cut(s) 530, 1180
MboII GAAGA 7 cut(s) 70, 106, 204, 246, 255, 1750, 1794
MfeI CAATTG 1 cut(s) 1484
MflI RGATCY 1 cut(s) 530
MhlI GDGCHC 6 cut(s) 363, 561, 743, 935, 1013, 1371
MlsI TGGCCA 1 cut(s) 1242
MluCI AATT 6 cut(s) 21, 96, 228, 237, 1484, 1581
MluNI TGGCCA 1 cut(s) 1242
MlyI GAGTC 5 cut(s) 344, 706, 1146, 1218, 1699
MmeI TCCRAC 2 cut(s) 340, 904
Mox20I TGGCCA 1 cut(s) 1242
MscI TGGCCA 1 cut(s) 1242
MseI TTAA 1 cut(s) 227
MslI CAYNNNNRTG 1 cut(s) 1410
Msp20I TGGCCA 1 cut(s) 1242
MspA1I CMGCKG 1 cut(s) 1376
MspI CCGG 7 cut(s) 375, 452, 578, 1299, 1349, 1629, 1667
MspR9I CCNGG 5 cut(s) 349, 376, 453, 610, 1511
MunI CAATTG 1 cut(s) 1484
MvaI CCWGG 3 cut(s) 349, 610, 1511
MvnI CGCG 2 cut(s) 541, 1156
MwoI GCNNNNNNNGC 4 cut(s) 757, 1342, 1385, 1515
NciI CCSGG 2 cut(s) 376, 453
NcoI CCATGG 1 cut(s) 1605
NdeII GATC 2 cut(s) 530, 1180
NlaIII CATG 6 cut(s) 697, 715, 1530, 1565, 1609, 1642
NlaIV GGNNCC 9 cut(s) 418, 741, 816, 828, 934, 993, 1103, 1175, 1331
NmeAIII GCCGAG 1 cut(s) 1202
NmuCI GTSAC 7 cut(s) 336, 970, 983, 1398, 1662, 1684, 1773
NspI RCATGY 2 cut(s) 715, 1642
NspV TTCGAA 1 cut(s) 473
OliI CACNNNNGTG 1 cut(s) 1410
PaeI GCATGC 1 cut(s) 1642
PaqCI CACCTGC 1 cut(s) 653
PceI AGGCCT 2 cut(s) 171, 572
PciI ACATGT 1 cut(s) 711
PcsI WCGNNNNNNNCGW 2 cut(s) 497, 561
PfeI GAWTC 5 cut(s) 404, 516, 1576, 1713, 1792
PflMI CCANNNNNTGG 2 cut(s) 1321, 1395
PfoI TCCNGGA 1 cut(s) 374
PkrI GCNGC 4 cut(s) 509, 543, 685, 1378
PleI GAGTC 5 cut(s) 343, 705, 1145, 1217, 1699
PpsI GAGTC 5 cut(s) 343, 705, 1145, 1217, 1699
PscI ACATGT 1 cut(s) 711
PshAI GACNNNNGTC 2 cut(s) 1136, 1682
PshBI ATTAAT 1 cut(s) 227
Psp6I CCWGG 3 cut(s) 347, 608, 1509
PspEI GGTNACC 1 cut(s) 1398
PspFI CCCAGC 4 cut(s) 69, 279, 701, 1694
PspGI CCWGG 3 cut(s) 347, 608, 1509
PspN4I GGNNCC 9 cut(s) 418, 741, 816, 828, 934, 993, 1103, 1175, 1331
PspOMI GGGCCC 1 cut(s) 739
PspPI GGNCC 6 cut(s) 739, 740, 900, 992, 1102, 1272
PsrI GAACNNNNNNTAC 2 cut(s) 737, 769
PstI CTGCAG 3 cut(s) 530, 664, 1381
PsuI RGATCY 1 cut(s) 530
PvuII CAGCTG 1 cut(s) 1376
RsaI GTAC 1 cut(s) 1652
RsaNI GTAC 1 cut(s) 1651
RseI CAYNNNNRTG 1 cut(s) 1410
SaqAI TTAA 1 cut(s) 227
SatI GCNGC 4 cut(s) 508, 542, 684, 1377
Sau3AI GATC 2 cut(s) 530, 1180
Sau96I GGNCC 6 cut(s) 739, 740, 900, 992, 1102, 1272
SbfI CCTGCAGG 1 cut(s) 664
SchI GAGTC 5 cut(s) 344, 706, 1146, 1218, 1699
ScrFI CCNGG 5 cut(s) 349, 376, 453, 610, 1511
SdaI CCTGCAGG 1 cut(s) 664
SduI GDGCHC 6 cut(s) 363, 561, 743, 935, 1013, 1371
SfaNI GCATC 6 cut(s) 321, 965, 1129, 1304, 1433, 1503
SfcI CTRYAG 3 cut(s) 526, 660, 1377
SfuI TTCGAA 1 cut(s) 473
SgrAI CRCCGGYG 1 cut(s) 577
SinI GGWCC 3 cut(s) 900, 1102, 1272
SmiMI CAYNNNNRTG 1 cut(s) 1410
SphI GCATGC 1 cut(s) 1642
Sse8387I CCTGCAGG 1 cut(s) 664
Sse9I AATT 6 cut(s) 21, 96, 228, 237, 1484, 1581
SseBI AGGCCT 2 cut(s) 171, 572
SspMI CTAG 2 cut(s) 147, 1778
StuI AGGCCT 2 cut(s) 171, 572
StyD4I CCNGG 5 cut(s) 347, 374, 451, 608, 1509
StyI CCWWGG 2 cut(s) 810, 1605
TaaI ACNGT 4 cut(s) 94, 105, 782, 981
TaiI ACGT 3 cut(s) 554, 1725, 1751
TasI AATT 6 cut(s) 21, 96, 228, 237, 1484, 1581
TauI GCSGC 1 cut(s) 544
TfiI GAWTC 5 cut(s) 404, 516, 1576, 1713, 1792
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TscAI CASTG 7 cut(s) 13, 130, 787, 943, 1097, 1693, 1758
TseFI GTSAC 7 cut(s) 336, 970, 983, 1398, 1662, 1684, 1773
TseI GCWGC 3 cut(s) 507, 683, 1376
Tsp45I GTSAC 7 cut(s) 336, 970, 983, 1398, 1662, 1684, 1773
TspDTI ATGAA 3 cut(s) 170, 508, 1026
TspGWI ACGGA 2 cut(s) 397, 743
TspRI CASTG 7 cut(s) 13, 130, 787, 943, 1097, 1693, 1758
Van91I CCANNNNNTGG 2 cut(s) 1321, 1395
VpaK11BI GGWCC 3 cut(s) 900, 1102, 1272
VspI ATTAAT 1 cut(s) 227
XbaI TCTAGA 1 cut(s) 146
XceI RCATGY 2 cut(s) 715, 1642
XspI CTAG 2 cut(s) 147, 1778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.