pycom01g16250

serine-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013211
Physical Location & Seq
Reverse (-)
15901154 .. 15902643
1490 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g16250.2

Sequence Viewer

Length: 621 bp
ATGGGCGGTGGGAAGGACAAGCAGCACGAAGACGAAGCAGCAGACAAGGGGCTTTTTAGTCAACTTGCACACGGACTCGGAGGAGGTTCTTACCCGCCTCAGCATGGGTACCCTTCCCAGCACGGCGGCTACCCTCCCCAGCAAGGCGGCTACCCGCCCCAGCACGGCGGCTATCCTCCCCAGCAGGGCGGCTACCCTCCACAAGGTTACCCGCCCCAGCAGGGTGGATATCCACCGCAAGGGTACCCGCCGCAGCAGGGATATCCTCCCCAAGGATACCCGCCTCAACAGGGGTACCCACCGTCTGGGTATCCTCAGCCTGGCCATCAGGGCTCATCTGGACACCATGGCGTAGGGACGTTGCTGGCTGGTGGTGCAGCCGCTGCTGCAGCTGCTGCAGGTGTTCACCATCTCACTCAAGGTGGTCAGCATGGCCAAGGTTCTCACGGTTCTTATGGTTCTCATGGTATGCTTCCTGGCGGTTATGGTGCTCACAATGTTGGTCACGGCTTTGCATCACATGGCGGGAAGCATGGCAAGTTCAAGCAGGGGAAATTCGGGAAGCATAAGAAAGGCAAGTTCGGCAAGCACGGTGGAGGCAAGTTCAAGAAATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trypsin

Protein Analysis

207

Amino Acids

21.07

Weight (kDa)

9.68

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013211)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19200
fragaria_vesca FvH4_7g23640
malus_domestica MD01G1142300.v1.1 MD07G1208000.v1.1
prunus_persica Prupe.2G243300_v2.0.a1
pyrus_communis pycom01g16250 pycom07g19370
rosa_chinensis RchiOBHm_Chr1g0368911
rosa_laevigata RLG00000027177
rosa_multiflora Rmu_sc0004250.1_g000044 Rmu_sc0012648.1_g000003
rosa_roxburghii Rroxscaffold_4G00288210
rosa_rugosa Rorug01G0345800
rosa_samantha Rh1AG352800 Rh1BG316300 Rh1CG331000 Rh1DG347800
rosa_wichuraiana Rw1G031140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 389
Acc36I ACCTGC 1 cut(s) 389
Acc65I GGTACC 3 cut(s) 108, 243, 294
AccB1I GGYRCC 3 cut(s) 108, 243, 294
AccB7I CCANNNNNTGG 1 cut(s) 305
AcoI YGGCCR 2 cut(s) 322, 433
AcsI RAATTY 1 cut(s) 554
AfaI GTAC 3 cut(s) 110, 245, 296
AgsI TTSAA 2 cut(s) 544, 607
AjnI CCWGG 2 cut(s) 319, 475
AluBI AGCT 1 cut(s) 392
AluI AGCT 1 cut(s) 392
Alw21I GWGCWC 1 cut(s) 493
AlwNI CAGNNNCTG 2 cut(s) 383, 395
AoxI GGCC 2 cut(s) 322, 433
ApeKI GCWGC 9 cut(s) 22, 38, 253, 377, 383, 386, 389, 392, 395
ApoI RAATTY 1 cut(s) 554
Asp718I GGTACC 3 cut(s) 108, 243, 294
AsuHPI GGTGA 1 cut(s) 398
BaeI ACNNNNGTAYC 2 cut(s) 278, 311
BalI TGGCCA 2 cut(s) 324, 435
BanI GGYRCC 3 cut(s) 108, 243, 294
BanII GRGCYC 1 cut(s) 335
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 493
BbvCI CCTCAGC 2 cut(s) 99, 315
BbvI GCAGC 9 cut(s) 34, 50, 265, 370, 373, 379, 382, 389, 401
BccI CCATC 2 cut(s) 333, 417
BceAI ACGGC 3 cut(s) 139, 181, 523
BciT130I CCWGG 2 cut(s) 321, 477
BciVI GTATCC 2 cut(s) 269, 321
BfmI CTRYAG 2 cut(s) 387, 396
BfuAI ACCTGC 1 cut(s) 389
BfuI GTATCC 2 cut(s) 269, 321
BglI GCCNNNNNGGC 1 cut(s) 330
Bme1390I CCNGG 2 cut(s) 321, 477
BmiI GGNNCC 3 cut(s) 110, 245, 296
BmrFI CCNGG 2 cut(s) 321, 477
BmsI GCATC 1 cut(s) 524
BpiI GAAGAC 1 cut(s) 36
Bpu10I CCTNAGC 2 cut(s) 99, 315
BpuEI CTTGAG 1 cut(s) 402
BsaJI CCNNGG 3 cut(s) 271, 346, 436
BseBI CCWGG 2 cut(s) 321, 477
BseDI CCNNGG 3 cut(s) 271, 346, 436
BseMII CTCAG 2 cut(s) 113, 329
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 9 cut(s) 34, 50, 265, 370, 373, 379, 382, 389, 401
BseYI CCCAGC 5 cut(s) 117, 138, 159, 180, 216
BsgI GTGCAG 1 cut(s) 396
BshFI GGCC 2 cut(s) 324, 435
BshNI GGYRCC 3 cut(s) 108, 243, 294
BsiHKAI GWGCWC 1 cut(s) 493
BslFI GGGAC 1 cut(s) 370
BsmFI GGGAC 1 cut(s) 370
BsnI GGCC 2 cut(s) 324, 435
Bsp1286I GDGCHC 2 cut(s) 335, 493
Bsp19I CCATGG 1 cut(s) 346
BspANI GGCC 2 cut(s) 324, 435
BspCNI CTCAG 2 cut(s) 112, 328
BspLI GGNNCC 3 cut(s) 110, 245, 296
BspMAI CTGCAG 2 cut(s) 391, 400
BspMI ACCTGC 1 cut(s) 389
BspT107I GGYRCC 3 cut(s) 108, 243, 294
BssECI CCNNGG 3 cut(s) 271, 346, 436
BssT1I CCWWGG 3 cut(s) 271, 346, 436
Bst2UI CCWGG 2 cut(s) 321, 477
Bst4CI ACNGT 3 cut(s) 303, 449, 593
BstAPI GCANNNNNTGC 2 cut(s) 383, 395
BstC8I GCNNGC 2 cut(s) 366, 587
BstDEI CTNAG 2 cut(s) 99, 315
BstDSI CCRYGG 1 cut(s) 346
BstEII GGTNACC 1 cut(s) 206
BstENI CCTNNNNNAGG 2 cut(s) 201, 270
BstMWI GCNNNNNNNGC 8 cut(s) 330, 374, 383, 386, 389, 392, 395, 582
BstNI CCWGG 2 cut(s) 321, 477
BstPI GGTNACC 1 cut(s) 206
BstSCI CCNGG 2 cut(s) 319, 475
BstSFI CTRYAG 2 cut(s) 387, 396
BstV1I GCAGC 9 cut(s) 34, 50, 265, 370, 373, 379, 382, 389, 401
BstV2I GAAGAC 1 cut(s) 36
BstXI CCANNNNNNTGG 1 cut(s) 224
BsuI GTATCC 2 cut(s) 269, 321
BsuRI GGCC 2 cut(s) 324, 435
BtgI CCRYGG 1 cut(s) 346
BveI ACCTGC 1 cut(s) 389
Cac8I GCNNGC 2 cut(s) 366, 587
CaiI CAGNNNCTG 2 cut(s) 383, 395
Csp6I GTAC 3 cut(s) 109, 244, 295
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviAII CATG 6 cut(s) 104, 347, 431, 464, 521, 533
CviQI GTAC 3 cut(s) 109, 244, 295
DdeI CTNAG 2 cut(s) 99, 315
EaeI YGGCCR 2 cut(s) 322, 433
Eco130I CCWWGG 3 cut(s) 271, 346, 436
Eco24I GRGCYC 1 cut(s) 335
Eco32I GATATC 2 cut(s) 230, 263
Eco91I GGTNACC 1 cut(s) 206
EcoNI CCTNNNNNAGG 2 cut(s) 201, 270
EcoO65I GGTNACC 1 cut(s) 206
EcoRII CCWGG 2 cut(s) 319, 475
EcoRV GATATC 2 cut(s) 230, 263
EcoT14I CCWWGG 3 cut(s) 271, 346, 436
EcoT38I GRGCYC 1 cut(s) 335
ErhI CCWWGG 3 cut(s) 271, 346, 436
FaeI CATG 6 cut(s) 107, 350, 434, 467, 524, 536
FaqI GGGAC 1 cut(s) 370
FatI CATG 6 cut(s) 103, 346, 430, 463, 520, 532
FauI CCCGC 6 cut(s) 102, 162, 219, 255, 288, 518
FriOI GRGCYC 1 cut(s) 335
GsaI CCCAGC 5 cut(s) 121, 142, 163, 184, 220
HaeIII GGCC 2 cut(s) 324, 435
Hin1II CATG 6 cut(s) 107, 350, 434, 467, 524, 536
HincII GTYRAC 1 cut(s) 62
HindII GTYRAC 1 cut(s) 62
HinfI GANTC 1 cut(s) 75
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 2 cut(s) 62, 406
Hpy188I TCNGA 1 cut(s) 80
Hpy188III TCNNGA 3 cut(s) 339, 559, 607
Hpy8I GTNNAC 2 cut(s) 62, 406
HpyAV CCTTC 2 cut(s) 7, 123
HpyCH4III ACNGT 3 cut(s) 303, 449, 593
HpyCH4IV ACGT 1 cut(s) 359
HpyCH4V TGCA 5 cut(s) 68, 377, 389, 398, 515
HpyF10VI GCNNNNNNNGC 8 cut(s) 330, 374, 383, 386, 389, 392, 395, 582
HpyF3I CTNAG 2 cut(s) 99, 315
HpySE526I ACGT 1 cut(s) 359
Hsp92II CATG 6 cut(s) 107, 350, 434, 467, 524, 536
KpnI GGTACC 3 cut(s) 112, 247, 298
Lsp1109I GCAGC 9 cut(s) 34, 50, 265, 370, 373, 379, 382, 389, 401
LweI GCATC 1 cut(s) 524
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 2 cut(s) 206, 503
MboII GAAGA 1 cut(s) 41
MhlI GDGCHC 2 cut(s) 335, 493
MlsI TGGCCA 2 cut(s) 324, 435
MluCI AATT 1 cut(s) 554
MluNI TGGCCA 2 cut(s) 324, 435
MlyI GAGTC 1 cut(s) 69
Mox20I TGGCCA 2 cut(s) 324, 435
MscI TGGCCA 2 cut(s) 324, 435
Msp20I TGGCCA 2 cut(s) 324, 435
MspA1I CMGCKG 2 cut(s) 383, 392
MspR9I CCNGG 2 cut(s) 321, 477
MvaI CCWGG 2 cut(s) 321, 477
MwoI GCNNNNNNNGC 8 cut(s) 330, 374, 383, 386, 389, 392, 395, 582
NcoI CCATGG 1 cut(s) 346
NlaIII CATG 6 cut(s) 107, 350, 434, 467, 524, 536
NlaIV GGNNCC 3 cut(s) 110, 245, 296
NmuCI GTSAC 1 cut(s) 503
PaqCI CACCTGC 1 cut(s) 389
PflMI CCANNNNNTGG 1 cut(s) 305
PleI GAGTC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 69
Psp6I CCWGG 2 cut(s) 319, 475
PspEI GGTNACC 1 cut(s) 206
PspFI CCCAGC 5 cut(s) 117, 138, 159, 180, 216
PspGI CCWGG 2 cut(s) 319, 475
PspN4I GGNNCC 3 cut(s) 110, 245, 296
PstI CTGCAG 2 cut(s) 391, 400
PstNI CAGNNNCTG 2 cut(s) 383, 395
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 3 cut(s) 110, 245, 296
RsaNI GTAC 3 cut(s) 109, 244, 295
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 2 cut(s) 321, 477
SduI GDGCHC 2 cut(s) 335, 493
SetI ASST 7 cut(s) 88, 208, 362, 394, 403, 424, 442
SfaNI GCATC 1 cut(s) 524
SfcI CTRYAG 2 cut(s) 387, 396
SmlI CTYRAG 1 cut(s) 417
SmoI CTYRAG 1 cut(s) 417
Sse9I AATT 1 cut(s) 554
StyD4I CCNGG 2 cut(s) 319, 475
StyI CCWWGG 3 cut(s) 271, 346, 436
TaaI ACNGT 3 cut(s) 303, 449, 593
TaiI ACGT 1 cut(s) 362
TasI AATT 1 cut(s) 554
TauI GCSGC 6 cut(s) 129, 150, 171, 192, 253, 383
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 9 cut(s) 22, 38, 253, 377, 383, 386, 389, 392, 395
Tsp45I GTSAC 1 cut(s) 503
TspGWI ACGGA 1 cut(s) 87
Van91I CCANNNNNTGG 1 cut(s) 305
XagI CCTNNNNNAGG 2 cut(s) 201, 270
XapI RAATTY 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.