pycom01g17810

Uroporphyrinogen decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012047
Physical Location & Seq
Forward (+)
17014848 .. 17017308
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
ATGGCTTGCATTCACAGCTACGGCTCCATAGCTTCCATCTCTCTTCCCACCTCGCCTTCGTTCTCGCCAAGATCCAGATGCGCCTACAAGCCTCGCTTTCTCTGCGCCCTCGGAGGGAGTGTGGCAGAACCCCAAGTTGCCTCCGTTGCCGAGCCTCTTTTGCTCAATGCAGTTCGCGGGGACGATGTTGAGAGACCCCCAGTTTGGCTCATGAGGCAAGCAGGGAGGTACATGAAGAGTTACCAAACTCTCTGCGAGAGGTACCCTTCGTTTCGAGAAAGATCTGAAAATGTTGACCTCATTGTCGAAATTTCGCTACAGCCCTGGAAGGTCTTCAAGCCTGATGGTGTCATTTTGTTCTCGGATATTCTAACTCCTCTTTCCGGGATGAATATACCATTTGACATTGTAAAAGGTAAGGGTCCTGTAATATTCAATCCTCTGCATACTGCTGATGATATTGATCAAGTAAGAGAGTTTGTTCCACAAGAATCAGTTCCGTATGTTGGGGAAGCATTGACAATCTTGCGAAAGGAGGTAGATAATAAGGCAGCAGTGCTAGGTTTTGTCGGGGCTCCTTTCACCCTAGCATCGTATGTGGTGGAAGGTGGTTCATCAAAGCATTTCTCAAACATAAAGAGATTAGCCTTCTCTCAACCAAAGGTCCTCCATGCATTACTTCAGAAATTTACAACTTCGATGACAAAGTATATTCGATACCAAGCTGATAATGGAGCTCAAGCCGTTCAGATCTTTGACTCATGGGCCACAGAACTCAGCCCTGTGGATTTTGAGGAATTCAGTCTGCCATACTTGAAGAAAATCGTGGATGCTGTGAAAGAGACCCATCCAGATCTCCCTTTAATCCTTTACGCAAGTGGATCTGGGGGTTTGCTTGAGAGGCTAGCTTTGACCGGTGTTGATGTAGTTAGCTTGGACTGGACTGTTGATATGGCGGAAGGTAGAAGGCGACTGGGACCAAATATAGCAGTTCAAGGTCTTGATGGATCCCGGTGTTCTTTTTGGTTCGAAAGAATTCATCACCAGTAG

Protein Analysis

350

Amino Acids

38.74

Weight (kDa)

6.4

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
URO-D PF01208 53 - 333 1.1e-101 Uroporphyrinogen decarboxylase (URO-D)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40490
fragaria_vesca FvH4_7g25624 FvH4_7g25624 FvH4_7g25624
malus_domestica MD01G1163500.v1.1 MD07G1231500.v1.1
prunus_persica Prupe.2G261500_v2.0.a1
pyrus_communis pycom01g17810 pycom07g20930
rosa_chinensis RchiOBHm_Chr1g0371761
rosa_laevigata RLG00000026943
rosa_multiflora Rmu_sc0001755.1_g000052
rosa_roxburghii Rroxscaffold_4G00285440
rosa_rugosa Rorug01G0366300
rosa_samantha Rh1AG376200 Rh1BG339600 Rh1CG352700 Rh1DG370800
rosa_wichuraiana Rw1G033100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 302
Acc65I GGTACC 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 261
AccII CGCG 1 cut(s) 177
AciI CCGC 2 cut(s) 177, 956
AclWI GGATC 4 cut(s) 66, 889, 1002, 1015
AcsI RAATTY 4 cut(s) 309, 686, 797, 1035
AcuI CTGAAG 1 cut(s) 665
AfaI GTAC 2 cut(s) 230, 263
AfiI CCNNNNNNNGG 4 cut(s) 114, 204, 383, 506
AgeI ACCGGT 1 cut(s) 914
AgsI TTSAA 4 cut(s) 337, 436, 817, 995
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 6 cut(s) 18, 32, 725, 737, 908, 933
AluI AGCT 6 cut(s) 18, 32, 725, 737, 908, 933
Alw21I GWGCWC 1 cut(s) 739
Alw26I GTCTC 2 cut(s) 187, 836
AlwI GGATC 4 cut(s) 66, 889, 1002, 1015
AoxI GGCC 1 cut(s) 765
ApeKI GCWGC 1 cut(s) 551
ApoI RAATTY 4 cut(s) 309, 686, 797, 1035
ArsI GACNNNNNNTTYG 2 cut(s) 186, 218
AsiGI ACCGGT 1 cut(s) 914
Asp700I GAANNNNTTC 3 cut(s) 332, 495, 1035
Asp718I GGTACC 1 cut(s) 261
AspLEI GCGC 2 cut(s) 83, 107
AspS9I GGNCC 4 cut(s) 422, 664, 765, 977
AsuC2I CCSGG 2 cut(s) 385, 1012
AsuHPI GGTGA 2 cut(s) 574, 1034
AsuII TTCGAA 1 cut(s) 1029
AsuNHI GCTAGC 1 cut(s) 904
AvaII GGWCC 3 cut(s) 422, 664, 977
BamHI GGATCC 1 cut(s) 1007
BanI GGYRCC 1 cut(s) 261
BanII GRGCYC 2 cut(s) 577, 739
BbsI GAAGAC 1 cut(s) 325
Bbv12I GWGCWC 1 cut(s) 739
BbvI GCAGC 1 cut(s) 563
BccI CCATC 4 cut(s) 44, 338, 855, 998
BceAI ACGGC 2 cut(s) 37, 728
BciT130I CCWGG 1 cut(s) 325
BclI TGATCA 1 cut(s) 463
BcnI CCSGG 2 cut(s) 385, 1012
BcoDI GTCTC 2 cut(s) 187, 836
BfaI CTAG 3 cut(s) 560, 587, 905
BfmI CTRYAG 1 cut(s) 317
BglII AGATCT 3 cut(s) 281, 750, 853
BisI GCNGC 1 cut(s) 552
BlsI GCNGC 1 cut(s) 553
Bme1390I CCNGG 3 cut(s) 325, 385, 1012
Bme18I GGWCC 3 cut(s) 422, 664, 977
BmgT120I GGNCC 4 cut(s) 422, 664, 765, 977
BmiI GGNNCC 6 cut(s) 25, 263, 423, 576, 978, 1009
BmrFI CCNGG 3 cut(s) 325, 385, 1012
BmrI ACTGGG 2 cut(s) 194, 983
BmsI GCATC 3 cut(s) 68, 599, 820
BmtI GCTAGC 1 cut(s) 908
BmuI ACTGGG 2 cut(s) 194, 983
BpiI GAAGAC 1 cut(s) 325
Bpu14I TTCGAA 1 cut(s) 1029
BpuEI CTTGAG 2 cut(s) 723, 917
BpuMI CCSGG 2 cut(s) 385, 1012
BsaBI GATNNNNATC 1 cut(s) 462
BsaI GGTCTC 2 cut(s) 187, 836
BsaJI CCNNGG 2 cut(s) 109, 323
BsaWI WCCGGW 1 cut(s) 914
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 4 cut(s) 114, 204, 383, 506
Bse118I RCCGGY 1 cut(s) 914
Bse1I ACTGG 4 cut(s) 200, 944, 978, 1045
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 2 cut(s) 109, 323
BseGI GGATG 3 cut(s) 393, 835, 847
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 4 cut(s) 114, 204, 383, 506
BseMII CTCAG 1 cut(s) 790
BseNI ACTGG 4 cut(s) 200, 944, 978, 1045
BseRI GAGGAG 1 cut(s) 366
BseXI GCAGC 1 cut(s) 563
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 1 cut(s) 767
BshNI GGYRCC 1 cut(s) 261
BshTI ACCGGT 1 cut(s) 914
BsiHKAI GWGCWC 1 cut(s) 739
BsiSI CCGG 3 cut(s) 384, 915, 1012
BslFI GGGAC 2 cut(s) 194, 990
BslI CCNNNNNNNGG 4 cut(s) 114, 204, 383, 506
BsmAI GTCTC 2 cut(s) 187, 836
BsmFI GGGAC 2 cut(s) 194, 990
BsmI GAATGC 1 cut(s) 9
BsnI GGCC 1 cut(s) 767
Bso31I GGTCTC 2 cut(s) 187, 836
Bsp119I TTCGAA 1 cut(s) 1029
Bsp1286I GDGCHC 2 cut(s) 577, 739
Bsp143I GATC 7 cut(s) 71, 281, 463, 750, 853, 881, 1007
BspACI CCGC 2 cut(s) 177, 956
BspANI GGCC 1 cut(s) 767
BspCNI CTCAG 1 cut(s) 789
BspFNI CGCG 1 cut(s) 177
BspHI TCATGA 1 cut(s) 210
BspLI GGNNCC 6 cut(s) 25, 263, 423, 576, 978, 1009
BspOI GCTAGC 1 cut(s) 908
BspPI GGATC 4 cut(s) 66, 889, 1002, 1015
BspT104I TTCGAA 1 cut(s) 1029
BspT107I GGYRCC 1 cut(s) 261
BspTNI GGTCTC 2 cut(s) 187, 836
BsrFI RCCGGY 1 cut(s) 914
BsrI ACTGG 4 cut(s) 200, 944, 978, 1045
BssAI RCCGGY 1 cut(s) 914
BssECI CCNNGG 2 cut(s) 109, 323
BssMI GATC 7 cut(s) 71, 281, 463, 750, 853, 881, 1007
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 1 cut(s) 946
Bst6I CTCTTC 2 cut(s) 48, 230
BstBI TTCGAA 1 cut(s) 1029
BstC8I GCNNGC 3 cut(s) 7, 219, 906
BstDEI CTNAG 1 cut(s) 776
BstF5I GGATG 3 cut(s) 393, 835, 847
BstFNI CGCG 1 cut(s) 177
BstHHI GCGC 2 cut(s) 83, 107
BstKTI GATC 7 cut(s) 74, 284, 466, 753, 856, 884, 1010
BstMAI GTCTC 2 cut(s) 187, 836
BstMBI GATC 7 cut(s) 71, 281, 463, 750, 853, 881, 1007
BstMWI GCNNNNNNNGC 6 cut(s) 15, 102, 146, 160, 214, 901
BstNI CCWGG 1 cut(s) 325
BstSCI CCNGG 3 cut(s) 323, 383, 1010
BstSFI CTRYAG 1 cut(s) 317
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 1 cut(s) 563
BstV2I GAAGAC 1 cut(s) 325
BstX2I RGATCY 6 cut(s) 71, 281, 750, 853, 881, 1007
BstYI RGATCY 6 cut(s) 71, 281, 750, 853, 881, 1007
BsuRI GGCC 1 cut(s) 767
BtsCI GGATG 3 cut(s) 393, 835, 847
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 1 cut(s) 561
Cac8I GCNNGC 3 cut(s) 7, 219, 906
CciI TCATGA 1 cut(s) 210
CfoI GCGC 2 cut(s) 83, 107
Cfr10I RCCGGY 1 cut(s) 914
Cfr13I GGNCC 4 cut(s) 422, 664, 765, 977
Csp6I GTAC 2 cut(s) 229, 262
CspAI ACCGGT 1 cut(s) 914
CviAII CATG 4 cut(s) 211, 232, 671, 762
CviQI GTAC 2 cut(s) 229, 262
DdeI CTNAG 1 cut(s) 776
DpnI GATC 7 cut(s) 73, 283, 465, 752, 855, 883, 1009
DpnII GATC 7 cut(s) 71, 281, 463, 750, 853, 881, 1007
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
Eam1104I CTCTTC 2 cut(s) 48, 230
EarI CTCTTC 2 cut(s) 48, 230
EciI GGCGGA 1 cut(s) 971
Ecl136II GAGCTC 1 cut(s) 737
Eco24I GRGCYC 2 cut(s) 577, 739
Eco31I GGTCTC 2 cut(s) 187, 836
Eco47I GGWCC 3 cut(s) 422, 664, 977
Eco53kI GAGCTC 1 cut(s) 737
Eco57I CTGAAG 1 cut(s) 665
EcoICRI GAGCTC 1 cut(s) 737
EcoO109I RGGNCCY 2 cut(s) 422, 664
EcoRI GAATTC 2 cut(s) 797, 1035
EcoRII CCWGG 1 cut(s) 323
EcoT22I ATGCAT 1 cut(s) 676
EcoT38I GRGCYC 2 cut(s) 577, 739
FaeI CATG 4 cut(s) 214, 235, 674, 765
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FaqI GGGAC 2 cut(s) 194, 990
FatI CATG 4 cut(s) 210, 231, 670, 761
FauI CCCGC 1 cut(s) 170
FbaI TGATCA 1 cut(s) 463
Fnu4HI GCNGC 1 cut(s) 552
FokI GGATG 3 cut(s) 400, 834, 842
FriOI GRGCYC 2 cut(s) 577, 739
Fsp4HI GCNGC 1 cut(s) 552
FspBI CTAG 3 cut(s) 560, 587, 905
GlaI GCGC 2 cut(s) 82, 106
GluI GCNGC 1 cut(s) 552
HaeIII GGCC 1 cut(s) 767
HapII CCGG 3 cut(s) 384, 915, 1012
HhaI GCGC 2 cut(s) 83, 107
Hin1II CATG 4 cut(s) 214, 235, 674, 765
Hin6I GCGC 2 cut(s) 81, 105
HinP1I GCGC 2 cut(s) 81, 105
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 2 cut(s) 491, 758
HpaII CCGG 3 cut(s) 384, 915, 1012
HphI GGTGA 2 cut(s) 574, 1034
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 5 cut(s) 113, 286, 364, 684, 750
Hpy188III TCNNGA 5 cut(s) 75, 211, 275, 851, 1001
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 7 cut(s) 66, 276, 322, 599, 658, 953, 960
HpyCH4III ACNGT 1 cut(s) 946
HpyCH4V TGCA 4 cut(s) 9, 170, 445, 674
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 102, 146, 160, 214, 901
HpyF3I CTNAG 1 cut(s) 776
Hsp92II CATG 4 cut(s) 214, 235, 674, 765
HspAI GCGC 2 cut(s) 81, 105
KpnI GGTACC 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 7 cut(s) 71, 281, 463, 750, 853, 881, 1007
LmnI GCTCC 3 cut(s) 29, 580, 734
Lsp1109I GCAGC 1 cut(s) 563
LweI GCATC 3 cut(s) 68, 599, 820
MaeI CTAG 3 cut(s) 560, 587, 905
MaeIII GTNAC 1 cut(s) 239
MalI GATC 7 cut(s) 73, 283, 465, 752, 855, 883, 1009
MboI GATC 7 cut(s) 71, 281, 463, 750, 853, 881, 1007
MboII GAAGA 4 cut(s) 35, 247, 325, 829
MflI RGATCY 6 cut(s) 71, 281, 750, 853, 881, 1007
MhlI GDGCHC 2 cut(s) 577, 739
MluCI AATT 4 cut(s) 309, 686, 797, 1035
MlyI GAGTC 1 cut(s) 752
Mph1103I ATGCAT 1 cut(s) 676
MroXI GAANNNNTTC 3 cut(s) 332, 495, 1035
MseI TTAA 1 cut(s) 863
MspI CCGG 3 cut(s) 384, 915, 1012
MspR9I CCNGG 3 cut(s) 325, 385, 1012
Mva1269I GAATGC 1 cut(s) 9
MvaI CCWGG 1 cut(s) 325
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 6 cut(s) 15, 102, 146, 160, 214, 901
NciI CCSGG 2 cut(s) 385, 1012
NdeII GATC 7 cut(s) 71, 281, 463, 750, 853, 881, 1007
NheI GCTAGC 1 cut(s) 904
NlaIII CATG 4 cut(s) 214, 235, 674, 765
NlaIV GGNNCC 6 cut(s) 25, 263, 423, 576, 978, 1009
NmeAIII GCCGAG 1 cut(s) 175
NsiI ATGCAT 1 cut(s) 676
NspV TTCGAA 1 cut(s) 1029
PagI TCATGA 1 cut(s) 210
PctI GAATGC 1 cut(s) 9
PdmI GAANNNNTTC 3 cut(s) 332, 495, 1035
PfeI GAWTC 1 cut(s) 491
PfoI TCCNGGA 1 cut(s) 383
PinAI ACCGGT 1 cut(s) 914
PkrI GCNGC 1 cut(s) 553
PleI GAGTC 1 cut(s) 752
PpsI GAGTC 1 cut(s) 752
PpuMI RGGWCCY 2 cut(s) 422, 664
Psp124BI GAGCTC 1 cut(s) 739
Psp5II RGGWCCY 2 cut(s) 422, 664
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 6 cut(s) 25, 263, 423, 576, 978, 1009
PspPI GGNCC 4 cut(s) 422, 664, 765, 977
PspPPI RGGWCCY 2 cut(s) 422, 664
PsuI RGATCY 6 cut(s) 71, 281, 750, 853, 881, 1007
RsaI GTAC 2 cut(s) 230, 263
RsaNI GTAC 2 cut(s) 229, 262
SacI GAGCTC 1 cut(s) 739
SaqAI TTAA 1 cut(s) 863
SatI GCNGC 1 cut(s) 552
Sau3AI GATC 7 cut(s) 71, 281, 463, 750, 853, 881, 1007
Sau96I GGNCC 4 cut(s) 422, 664, 765, 977
SchI GAGTC 1 cut(s) 752
ScrFI CCNGG 3 cut(s) 325, 385, 1012
SduI GDGCHC 2 cut(s) 577, 739
SfaNI GCATC 3 cut(s) 68, 599, 820
SfcI CTRYAG 1 cut(s) 317
SfuI TTCGAA 1 cut(s) 1029
SinI GGWCC 3 cut(s) 422, 664, 977
SmlI CTYRAG 2 cut(s) 738, 896
SmoI CTYRAG 2 cut(s) 738, 896
Sse9I AATT 4 cut(s) 309, 686, 797, 1035
SsiI CCGC 2 cut(s) 177, 956
SspI AATATT 1 cut(s) 432
SspMI CTAG 3 cut(s) 560, 587, 905
SstI GAGCTC 1 cut(s) 739
StyD4I CCNGG 3 cut(s) 323, 383, 1010
TaaI ACNGT 1 cut(s) 946
TaqI TCGA 5 cut(s) 274, 306, 698, 715, 1029
TasI AATT 4 cut(s) 309, 686, 797, 1035
TfiI GAWTC 1 cut(s) 491
Tru1I TTAA 1 cut(s) 863
Tru9I TTAA 1 cut(s) 863
TscAI CASTG 1 cut(s) 561
TseI GCWGC 1 cut(s) 551
TspDTI ATGAA 4 cut(s) 248, 404, 603, 1028
TspGWI ACGGA 2 cut(s) 133, 489
TspRI CASTG 1 cut(s) 561
VpaK11BI GGWCC 3 cut(s) 422, 664, 977
XapI RAATTY 4 cut(s) 309, 686, 797, 1035
XcmI CCANNNNNNNNNTGG 1 cut(s) 728
XmnI GAANNNNTTC 3 cut(s) 332, 495, 1035
XspI CTAG 3 cut(s) 560, 587, 905
Zsp2I ATGCAT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.