pycom01g18090
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013923
Physical Location & Seq
Reverse (-)
17215570 .. 17219463
3894 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3141 bp
ATGATCTTGACGATCCTTAAATGGTACCCGTTGATTGTATATCATCTGCACATAATATACGATTTGAAAATTCCCGCAATCCTTACAAAAAAAATCCAAAGAAAATCCTTATTCATAAATAGGTCCCCATCTACCAGGCCGGTCCCAAACTCATTAGACTTTATACACTACGTACAGTCACAGACACACACAGAAGAGAGAGATAGAGAGAGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGTGAAGCTCTCAAGAAAGTTCCCTGAATACTCAAAGCCTTCAGCGACCCTTTTCAGGCATGAAAACGCTGCTCCTCCTATTCGCCGTCGTTGTTTTGTGTCCGGTTCTTGCGAGATCGATCTGAACCCGTCTCTGAACGACGACGTGTTGGGGCTGATAGTGTTCAAGGCGAATGTCCAAGATCCGAAGGGAAAGTTAGCGTCTTGGAGCGAAGACGACGACAGTGCTTGCAGTTGGGTCGGGGTAAAATGCCACCCGAGATCCAACCGGGTCATCGAGCTCAACCTCGACAACTTCTCGCTTTCGGGTCACATCGGGCGCGGCCTTCTGCAACTGCAAGCGCTCCGGAAGCTCTCGCTTTCGAAGAACAATCTCATCGGAAGCTTAACGCCTAACATTGCTCACATTGACAACCTCCGAGTTCTCGATTTGAGTGAGAACAGTTTCTCCGGTGATGTTCCAGAAGAGTTTTTCCGGCAATGTGGGTCACTGAGAGTGATTTCTCTGGCGAAAAACAAGTTTTCCGAGAAAATTCCGGAGAGTTTGGGCTCGTGTCCGAGTCTAGCTTCTGTTAACTTGTCGTTGAACCAGTTTTCGGGTTCGGTTCCGGCTGGGGTTTGGTCCTTGAGTGGGATTAGATCACTGGATTTGTCTGACAACTTGTTGGAGGGTGGAATTCCAAAGGGGGTTGAAGGATTGAATAATTTGCGAGGAATTAATTTGGCCAGGAATCGGTTTACTGGGCAAGTTCCAGATGGAATTGGAAACTGGTTGCTCTTGAGGTCCATTGATCTGAGCGAGAATTCGTTCTCGGGTAACCTCCCTCAGACAATGCAGAAACTTGGCCTGTGCAGCTATCTGAATTTGCACCAGAACACATTTTCCGGTGAGGTTCCGGAGTGGATTGGAGAGATGAAGAGCCTGGAGACTTTGGACCTTTCTAGCAATCGATTTTCGGGTGAAGTTCCAAGCTCAATAGGCAATCTTGAGTCTTTGAAAGTACTGAATTTTTCTGCAAATGGGTTCACTGGTAGCTTGCCAAAGTCTCTGTCTTATTGCACAAACCTTCTGGCTTTAGATTTTAGCAAGAATTCGATGGCGGGTGAGCTTCCTGTGTGGATACTCGCGGCAGGTACAGAGGAAGTTTCACTTTCAGAGAAAAAACTAGGTGGAAGCAAGGACATCCCTCTATCTTCATCGGCTGGAAATGCACTAAAGAATCTCCAAGTCTTGGATTTATCCCTTAATCATTTTTCGGGTGAAATTACGTCTGACATTGGGGCCTTAAGCAGCTTGCACGTTTTGAACCTCTCTGGTAACTCTCTTGCTGGTTCTATTCCTGTGGCCATTGGAGACTTGAAGGTCCTGGACAATCTCGACTTGAGCGAGAATCGGCTCAATGGAACCATTCCAACTTCAATCGAGAACTGTTCTTCTCTAACCACTTTAATTGTATCACAGAACAGATTGACTGGTCCAATACCTGCAGCAATGTCCAAACTTACCAACTTACAAAATGTGGACTTGTCTTTCAATAGCCTCACGGGAGGACTGCCGAAGCAGTTAGCCAATCTTCCCAACCTTCTTTCCTTCAAAATTTCCCACAACAACCTCCAGGGTGAACTACCTGCGGGTGCCTTTTTCAACACCATCTCCCCTTCCTCCGTCTCTGGAAATCCATCTCTCTGTGGTTCTGCAGTTAATAAGTCTTGCCCGGGAGTCCTTCCCAAACCCATTGTCCTCAATCCCAATTCCTCTTCTGACTCCAGCACAGGAGAAATCTCATCAAATCTTGGTCATCGAAGAATCTTACTCAGTATTTCTTCGCTAATTGCCATTGCTGCAGCCGCTGTCATTGTCATTGGTGTAATTGCCATTACTGTGCTTAATCTCCGTGTCCGGTCTTCCTCTTCCACAACTCAACCGGCGGCAGCCCTTGCATTTTCAGGTGGGGATGACTTCAGCCATTCCCCTACAACAGATGGAAACTCTGGCAAGCTTGTCATGTTCTCAGGTGAGCCTGACTTCAGCACCAGCGCACATGCACTGCTCAACAAGGACTGTGAGCTTGGGCGAGGTGGGTTTGGAGCAGTCTACCGGACTGTTCTTCGAGATGGACGACCAGTTGCAATCAAGAAGCTCACTGTTTCTAGTCTTGTCAAATCTCAAGGAGAATTTGAAAGGGAAGTGAAGAAATTGGGGAAAGTGAGGCACGATAATCTTGTGGAGATCGAAGGCTATTACTGGACTCCATCATTACAGCTCATCATATACGAATATGTTGCCGGTGGTAGTTTATACAAGCATCTTCATGATGGAGCAGGTGGAAACTTCCTGTCGTGGAATGACCGGTTCAACATAATTCTTGGAACTGCAAAAAGTTTGGCTCATTTGCACCAGATGAACATAATTCACTACAATATAAAGTCCAGCAATGTCCTGATTGGCAGCTCAGGCGAACCCAAAGTGGGAGACTTTGGCCTGGCTAGGTTGTTGCCAATGCTTGACCGATATGTTCTGAGCAGCAAGATTCAGAGCGCCCTTGGCTACATGGCACCAGAGTTTGCTTGCAAAACGGTAAAGATAACCGAGAAATGTGATGTGTACGGGTTTGGGATTTTGGTTCTAGAGATTGTCACTGGGAAAAGACCTGTCGAGTACATGGAAGATGATGTTGTGGTGCTTTGTGACATGGTGAGAGAAGCACTGGAAGAAGGCAGGGTAGAAGAATGCGTTGATGCTCGGCTCCAAGGAATTTTCCCAGCAGAAGAGGCTATACCTGTGATGAAACTAGGCTTGATATGCACGTCACAAGTGCCATCAAACAGACCAGACATGGGGGAGGTCGTCAACATTTTGGAGCTGATCCGATGTCCTTCCGAAGGCCAAGAGGAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1047

Amino Acids

113.49

Weight (kDa)

6.43

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 125 - 164 2.9e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 218 - 278 2.3e-07 Leucine rich repeat
LRR_14 PF23598 286 - 440 1.2e-11 Leucine-rich repeat region
LRR_14 PF23598 485 - 617 6.6e-12 Leucine-rich repeat region
LRR_8 PF13855 511 - 570 4.9e-06 Leucine rich repeat
LRR_8 PF13855 536 - 594 4.4e-07 Leucine rich repeat
LRR_8 PF13855 559 - 618 5.6e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 766 - 1031 7.6e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 767 - 971 1.7e-38 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013923)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56370
fragaria_vesca FvH4_7g25950
malus_domestica MD01G1167900.v1.1 MD07G1233900.v1.1
prunus_persica Prupe.2G264300_v2.0.a1
pyrus_communis pycom01g18090 pycom07g21170
rosa_chinensis RchiOBHm_Chr1g0372331
rosa_laevigata RLG00000026907
rosa_multiflora Rmu_sc0001755.1_g000008
rosa_roxburghii Rroxscaffold_4G00285050
rosa_rugosa Rorug01G0371200
rosa_samantha Rh1AG379600 Rh1BG343100 Rh1CG356600 Rh1DG374300
rosa_wichuraiana Rw1G033370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2552
AasI GACNNNNNNGTC 1 cut(s) 1601
Acc36I ACCTGC 4 cut(s) 1361, 1732, 1876, 2552
Acc65I GGTACC 1 cut(s) 24
AccB1I GGYRCC 3 cut(s) 24, 1874, 2794
AccB7I CCANNNNNTGG 1 cut(s) 1471
AccI GTMKAC 1 cut(s) 2334
AccII CGCG 2 cut(s) 561, 1367
AccIII TCCGGA 3 cut(s) 585, 775, 1135
AciI CCGC 7 cut(s) 75, 561, 1340, 1367, 1871, 2088, 2168
AclWI GGATC 4 cut(s) 7, 416, 495, 3100
AcoI YGGCCR 2 cut(s) 963, 1584
AcuI CTGAAG 3 cut(s) 264, 2185, 2251
AfaI GTAC 6 cut(s) 26, 174, 1242, 1375, 2846, 2900
AfeI AGCGCT 1 cut(s) 582
AfiI CCNNNNNNNGG 8 cut(s) 294, 835, 870, 972, 1471, 2708, 3077, 3122
AflII CTTAAG 1 cut(s) 1525
AflIII ACRYGT 1 cut(s) 384
AgeI ACCGGT 1 cut(s) 2589
AjiI CACGTC 2 cut(s) 385, 3048
AjnI CCWGG 6 cut(s) 134, 965, 1161, 1605, 1854, 2721
AloI GAACNNNNNNTCC 4 cut(s) 671, 703, 1106, 1138
Alw21I GWGCWC 1 cut(s) 522
Alw26I GTCTC 6 cut(s) 375, 1160, 1290, 1587, 1912, 2706
AlwI GGATC 4 cut(s) 7, 416, 495, 3100
AlwNI CAGNNNCTG 1 cut(s) 2090
Ama87I CYCGRG 3 cut(s) 496, 1051, 1954
Aor13HI TCCGGA 3 cut(s) 585, 775, 1135
Aor51HI AGCGCT 1 cut(s) 582
AoxI GGCC 8 cut(s) 137, 562, 963, 1084, 1521, 1584, 2719, 3124
ApeKI GCWGC 9 cut(s) 308, 1092, 1530, 1727, 2081, 2084, 2171, 2688, 2763
ArsI GACNNNNNNTTYG 4 cut(s) 1274, 1306, 1962, 1994
AseI ATTAAT 1 cut(s) 957
AsiGI ACCGGT 1 cut(s) 2589
Asp700I GAANNNNTTC 2 cut(s) 683, 1046
Asp718I GGTACC 1 cut(s) 24
AspLEI GCGC 4 cut(s) 561, 583, 2279, 2780
AspS9I GGNCC 8 cut(s) 123, 142, 861, 1023, 1174, 1521, 1603, 1715
AsuC2I CCSGG 3 cut(s) 509, 1955, 1956
AsuHPI GGTGA 8 cut(s) 704, 1139, 1211, 1355, 1511, 1871, 2267, 2947
AsuII TTCGAA 1 cut(s) 602
AvaI CYCGRG 3 cut(s) 496, 1051, 1954
AvaII GGWCC 7 cut(s) 123, 142, 861, 1023, 1174, 1603, 1715
BalI TGGCCA 2 cut(s) 965, 1586
BanI GGYRCC 3 cut(s) 24, 1874, 2794
BanII GRGCYC 2 cut(s) 522, 791
BarI GAAGNNNNNNTAC 2 cut(s) 3000, 3032
BauI CACGAG 1 cut(s) 790
BbsI GAAGAC 2 cut(s) 459, 2136
Bbv12I GWGCWC 1 cut(s) 522
BbvI GCAGC 9 cut(s) 295, 1104, 1542, 1739, 2068, 2096, 2183, 2700, 2775
BceAI ACGGC 1 cut(s) 309
BcgI CGANNNNNNTGC 6 cut(s) 446, 460, 480, 494, 2504, 2538
BciT130I CCWGG 6 cut(s) 136, 967, 1163, 1607, 1856, 2723
BciVI GTATCC 1 cut(s) 1353
BcnI CCSGG 3 cut(s) 509, 1955, 1956
BcoDI GTCTC 6 cut(s) 375, 1160, 1290, 1587, 1912, 2706
BfaI CTAG 7 cut(s) 803, 1182, 1406, 2391, 2727, 2867, 3032
BfmI CTRYAG 3 cut(s) 1725, 1935, 2082
BfoI RGCGCY 2 cut(s) 584, 2781
BfrI CTTAAG 1 cut(s) 1525
BfuAI ACCTGC 4 cut(s) 1361, 1732, 1876, 2552
BfuI GTATCC 1 cut(s) 1353
BmcAI AGTACT 1 cut(s) 1242
Bme1390I CCNGG 9 cut(s) 136, 509, 967, 1163, 1607, 1856, 1955, 1956, 2723
Bme18I GGWCC 7 cut(s) 123, 142, 861, 1023, 1174, 1603, 1715
BmeT110I CYCGRG 3 cut(s) 496, 1051, 1954
BmgBI CACGTC 2 cut(s) 385, 3048
BmgT120I GGNCC 8 cut(s) 123, 142, 861, 1023, 1174, 1521, 1603, 1715
BmrFI CCNGG 9 cut(s) 136, 509, 967, 1163, 1607, 1856, 1955, 1956, 2723
BmrI ACTGGG 2 cut(s) 990, 2889
BmsI GCATC 2 cut(s) 2554, 2968
BmuI ACTGGG 2 cut(s) 990, 2889
BpiI GAAGAC 2 cut(s) 459, 2136
BplI GAGNNNNNCTC 2 cut(s) 231, 263
BpmI CTGGAG 3 cut(s) 1184, 1838, 1990
Bpu10I CCTNAGC 1 cut(s) 2692
Bpu14I TTCGAA 1 cut(s) 602
BpuEI CTTGAG 6 cut(s) 235, 886, 1039, 1247, 1642, 2391
BpuMI CCSGG 3 cut(s) 509, 1955, 1956
Bsa29I ATCGAT 2 cut(s) 357, 1189
BsaAI YACGTR 1 cut(s) 172
BsaJI CCNNGG 4 cut(s) 1855, 1954, 2782, 2989
BsaWI WCCGGW 9 cut(s) 341, 585, 689, 775, 1124, 1135, 2139, 2337, 2589
BsaXI ACNNNNNCTCC 4 cut(s) 671, 701, 1877, 1907
Bsc4I CCNNNNNNNGG 8 cut(s) 294, 835, 870, 972, 1471, 2708, 3077, 3122
Bse118I RCCGGY 4 cut(s) 139, 2164, 2525, 2589
Bse3DI GCAATG 5 cut(s) 636, 725, 1737, 2076, 2680
BseAI TCCGGA 3 cut(s) 585, 775, 1135
BseBI CCWGG 6 cut(s) 136, 967, 1163, 1607, 1856, 2723
BseCI ATCGAT 2 cut(s) 357, 1189
BseDI CCNNGG 4 cut(s) 1855, 1954, 2782, 2989
BseGI GGATG 2 cut(s) 1422, 2200
BseLI CCNNNNNNNGG 8 cut(s) 294, 835, 870, 972, 1471, 2708, 3077, 3122
BseMI GCAATG 5 cut(s) 636, 725, 1737, 2076, 2680
BseMII CTCAG 7 cut(s) 722, 1025, 1079, 2068, 2265, 2706, 2750
BseRI GAGGAG 1 cut(s) 303
BseXI GCAGC 9 cut(s) 295, 1104, 1542, 1739, 2068, 2096, 2183, 2700, 2775
BseYI CCCAGC 2 cut(s) 851, 3001
BsgI GTGCAG 2 cut(s) 32, 1111
Bsh1236I CGCG 2 cut(s) 561, 1367
BshFI GGCC 8 cut(s) 139, 564, 965, 1086, 1523, 1586, 2721, 3126
BshNI GGYRCC 3 cut(s) 24, 1874, 2794
BshTI ACCGGT 1 cut(s) 2589
BshVI ATCGAT 2 cut(s) 357, 1189
BsiHKAI GWGCWC 1 cut(s) 522
BsiHKCI CYCGRG 3 cut(s) 496, 1051, 1954
BslFI GGGAC 2 cut(s) 109, 128
BslI CCNNNNNNNGG 8 cut(s) 294, 835, 870, 972, 1471, 2708, 3077, 3122
BsmAI GTCTC 6 cut(s) 375, 1160, 1290, 1587, 1912, 2706
BsmBI CGTCTC 2 cut(s) 375, 1912
BsmFI GGGAC 2 cut(s) 109, 128
BsmI GAATGC 1 cut(s) 2975
BsnI GGCC 8 cut(s) 139, 564, 965, 1086, 1523, 1586, 2721, 3126
BsoBI CYCGRG 3 cut(s) 496, 1051, 1954
Bsp119I TTCGAA 1 cut(s) 602
Bsp1286I GDGCHC 2 cut(s) 522, 791
Bsp13I TCCGGA 3 cut(s) 585, 775, 1135
BspACI CCGC 7 cut(s) 75, 561, 1340, 1367, 1871, 2088, 2168
BspANI GGCC 8 cut(s) 139, 564, 965, 1086, 1523, 1586, 2721, 3126
BspCNI CTCAG 7 cut(s) 723, 1026, 1078, 2067, 2264, 2705, 2751
BspDI ATCGAT 2 cut(s) 357, 1189
BspEI TCCGGA 3 cut(s) 585, 775, 1135
BspFNI CGCG 2 cut(s) 561, 1367
BspHI TCATGA 1 cut(s) 2551
BspMAI CTGCAG 3 cut(s) 1729, 1939, 2086
BspMI ACCTGC 4 cut(s) 1361, 1732, 1876, 2552
BspPI GGATC 4 cut(s) 7, 416, 495, 3100
BspQI GCTCTTC 1 cut(s) 1151
BspT104I TTCGAA 1 cut(s) 602
BspT107I GGYRCC 3 cut(s) 24, 1874, 2794
BspTI CTTAAG 1 cut(s) 1525
BsrDI GCAATG 5 cut(s) 636, 725, 1737, 2076, 2680
BsrFI RCCGGY 4 cut(s) 139, 2164, 2525, 2589
BssAI RCCGGY 4 cut(s) 139, 2164, 2525, 2589
BssECI CCNNGG 4 cut(s) 1855, 1954, 2782, 2989
BssSI CACGAG 1 cut(s) 790
BssT1I CCWWGG 2 cut(s) 2782, 2989
Bst2BI CACGAG 1 cut(s) 790
Bst2UI CCWGG 6 cut(s) 136, 967, 1163, 1607, 1856, 2723
Bst4CI ACNGT 9 cut(s) 177, 464, 683, 1670, 2122, 2303, 2344, 2386, 2818
Bst6I CTCTTC 6 cut(s) 189, 699, 1151, 2002, 2155, 3003
BstAFI CTTAAG 1 cut(s) 1525
BstAPI GCANNNNNTGC 1 cut(s) 2177
BstBAI YACGTR 1 cut(s) 172
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 6 cut(s) 469, 579, 1277, 1535, 2237, 2809
BstDEI CTNAG 7 cut(s) 731, 1034, 1065, 2054, 2251, 2692, 2759
BstEII GGTNACC 1 cut(s) 1055
BstENI CCTNNNNNAGG 1 cut(s) 3120
BstF5I GGATG 2 cut(s) 1422, 2200
BstFNI CGCG 2 cut(s) 561, 1367
BstH2I RGCGCY 2 cut(s) 584, 2781
BstHHI GCGC 4 cut(s) 561, 583, 2279, 2780
BstMAI GTCTC 6 cut(s) 375, 1160, 1290, 1587, 1912, 2706
BstNI CCWGG 6 cut(s) 136, 967, 1163, 1607, 1856, 2723
BstNSI RCATGY 1 cut(s) 2285
BstPI GGTNACC 1 cut(s) 1055
BstSCI CCNGG 9 cut(s) 134, 507, 965, 1161, 1605, 1854, 1953, 1954, 2721
BstSFI CTRYAG 3 cut(s) 1725, 1935, 2082
BstSNI TACGTA 1 cut(s) 172
BstUI CGCG 2 cut(s) 561, 1367
BstV1I GCAGC 9 cut(s) 295, 1104, 1542, 1739, 2068, 2096, 2183, 2700, 2775
BstV2I GAAGAC 2 cut(s) 459, 2136
BstX2I RGATCY 2 cut(s) 421, 500
BstYI RGATCY 2 cut(s) 421, 500
Bsu15I ATCGAT 2 cut(s) 357, 1189
BsuI GTATCC 1 cut(s) 1353
BsuRI GGCC 8 cut(s) 139, 564, 965, 1086, 1523, 1586, 2721, 3126
BsuTUI ATCGAT 2 cut(s) 357, 1189
BtrI CACGTC 2 cut(s) 385, 3048
BtsCI GGATG 2 cut(s) 1422, 2200
BtsI GCAGTG 1 cut(s) 2285
BtsIMutI CAGTG 8 cut(s) 469, 728, 881, 1266, 2285, 2382, 2877, 2945
BveI ACCTGC 4 cut(s) 1361, 1732, 1876, 2552
Cac8I GCNNGC 6 cut(s) 469, 579, 1277, 1535, 2237, 2809
CaiI CAGNNNCTG 1 cut(s) 2090
CciI TCATGA 1 cut(s) 2551
CfoI GCGC 4 cut(s) 561, 583, 2279, 2780
Cfr10I RCCGGY 4 cut(s) 139, 2164, 2525, 2589
Cfr13I GGNCC 8 cut(s) 123, 142, 861, 1023, 1174, 1521, 1603, 1715
Cfr9I CCCGGG 1 cut(s) 1954
ClaI ATCGAT 2 cut(s) 357, 1189
CseI GACGC 1 cut(s) 429
Csp6I GTAC 6 cut(s) 25, 173, 1241, 1374, 2845, 2899
CspAI ACCGGT 1 cut(s) 2589
CspCI CAANNNNNGTGG 2 cut(s) 1672, 1707
CviAII CATG 8 cut(s) 299, 2245, 2282, 2552, 2791, 2902, 2932, 3076
CviQI GTAC 6 cut(s) 25, 173, 1241, 1374, 2845, 2899
DdeI CTNAG 7 cut(s) 731, 1034, 1065, 2054, 2251, 2692, 2759
DrdI GACNNNNNNGTC 1 cut(s) 1601
DseDI GACNNNNNNGTC 1 cut(s) 1601
EaeI YGGCCR 2 cut(s) 963, 1584
Eam1104I CTCTTC 6 cut(s) 189, 699, 1151, 2002, 2155, 3003
EarI CTCTTC 6 cut(s) 189, 699, 1151, 2002, 2155, 3003
Ecl136II GAGCTC 1 cut(s) 520
Eco105I TACGTA 1 cut(s) 172
Eco130I CCWWGG 2 cut(s) 2782, 2989
Eco24I GRGCYC 2 cut(s) 522, 791
Eco47I GGWCC 7 cut(s) 123, 142, 861, 1023, 1174, 1603, 1715
Eco47III AGCGCT 1 cut(s) 582
Eco53kI GAGCTC 1 cut(s) 520
Eco57I CTGAAG 3 cut(s) 264, 2185, 2251
Eco88I CYCGRG 3 cut(s) 496, 1051, 1954
Eco91I GGTNACC 1 cut(s) 1055
EcoICRI GAGCTC 1 cut(s) 520
EcoNI CCTNNNNNAGG 1 cut(s) 3120
EcoO109I RGGNCCY 3 cut(s) 123, 1521, 1603
EcoO65I GGTNACC 1 cut(s) 1055
EcoRI GAATTC 3 cut(s) 915, 1042, 1330
EcoRII CCWGG 6 cut(s) 134, 965, 1161, 1605, 1854, 2721
EcoT14I CCWWGG 2 cut(s) 2782, 2989
EcoT38I GRGCYC 2 cut(s) 522, 791
ErhI CCWWGG 2 cut(s) 2782, 2989
Esp3I CGTCTC 2 cut(s) 375, 1912
FaeI CATG 8 cut(s) 302, 2248, 2285, 2555, 2794, 2905, 2935, 3079
FalI AAGNNNNNCTT 2 cut(s) 1374, 1406
FaqI GGGAC 2 cut(s) 109, 128
FatI CATG 8 cut(s) 298, 2244, 2281, 2551, 2790, 2901, 2931, 3075
FauI CCCGC 3 cut(s) 82, 1333, 1864
FblI GTMKAC 1 cut(s) 2334
FokI GGATG 2 cut(s) 1409, 2207
FriOI GRGCYC 2 cut(s) 522, 791
FspBI CTAG 7 cut(s) 803, 1182, 1406, 2391, 2727, 2867, 3032
GlaI GCGC 4 cut(s) 560, 582, 2278, 2779
GsaI CCCAGC 2 cut(s) 855, 3005
GsuI CTGGAG 3 cut(s) 1184, 1838, 1990
HaeII RGCGCY 2 cut(s) 584, 2781
HaeIII GGCC 8 cut(s) 139, 564, 965, 1086, 1523, 1586, 2721, 3126
HgaI GACGC 1 cut(s) 429
HhaI GCGC 4 cut(s) 561, 583, 2279, 2780
Hin1II CATG 8 cut(s) 302, 2248, 2285, 2555, 2794, 2905, 2935, 3079
Hin6I GCGC 4 cut(s) 559, 581, 2277, 2778
HinP1I GCGC 4 cut(s) 559, 581, 2277, 2778
HincII GTYRAC 2 cut(s) 814, 3091
HindII GTYRAC 2 cut(s) 814, 3091
HindIII AAGCTT 2 cut(s) 622, 2237
HpaI GTTAAC 1 cut(s) 814
HphI GGTGA 8 cut(s) 704, 1139, 1211, 1355, 1511, 1871, 2267, 2947
Hpy166II GTNNAC 8 cut(s) 814, 978, 1266, 1762, 1862, 2335, 2845, 3091
Hpy8I GTNNAC 8 cut(s) 814, 978, 1266, 1762, 1862, 2335, 2845, 3091
Hpy99I CGWCG 4 cut(s) 330, 383, 386, 461
HpyCH4III ACNGT 9 cut(s) 177, 464, 683, 1670, 2122, 2303, 2344, 2386, 2818
HpyCH4IV ACGT 5 cut(s) 171, 384, 1508, 1539, 3047
HpyF3I CTNAG 7 cut(s) 731, 1034, 1065, 2054, 2251, 2692, 2759
HpySE526I ACGT 5 cut(s) 171, 384, 1508, 1539, 3047
Hsp92II CATG 8 cut(s) 302, 2248, 2285, 2555, 2794, 2905, 2935, 3079
HspAI GCGC 4 cut(s) 559, 581, 2277, 2778
Kpn2I TCCGGA 3 cut(s) 585, 775, 1135
KpnI GGTACC 1 cut(s) 28
KspAI GTTAAC 1 cut(s) 814
LguI GCTCTTC 1 cut(s) 1151
LmnI GCTCC 7 cut(s) 316, 447, 588, 2327, 2558, 2991, 3100
Lsp1109I GCAGC 9 cut(s) 295, 1104, 1542, 1739, 2068, 2096, 2183, 2700, 2775
LweI GCATC 2 cut(s) 2554, 2968
MaeI CTAG 7 cut(s) 803, 1182, 1406, 2391, 2727, 2867, 3032
MaeII ACGT 5 cut(s) 171, 384, 1508, 1539, 3047
MaeIII GTNAC 8 cut(s) 177, 548, 726, 1055, 1556, 2875, 2927, 3048
MflI RGATCY 2 cut(s) 421, 500
MhlI GDGCHC 2 cut(s) 522, 791
MlsI TGGCCA 2 cut(s) 965, 1586
MluNI TGGCCA 2 cut(s) 965, 1586
MlyI GAGTC 5 cut(s) 808, 1238, 1968, 1997, 2482
MmeI TCCRAC 3 cut(s) 528, 885, 1676
Mox20I TGGCCA 2 cut(s) 965, 1586
MroI TCCGGA 3 cut(s) 585, 775, 1135
MroXI GAANNNNTTC 2 cut(s) 683, 1046
MscI TGGCCA 2 cut(s) 965, 1586
MseI TTAA 9 cut(s) 18, 626, 813, 957, 1485, 1526, 1688, 1941, 2127
MslI CAYNNNNRTG 2 cut(s) 2120, 2550
Msp20I TGGCCA 2 cut(s) 965, 1586
MspA1I CMGCKG 1 cut(s) 2090
MspCI CTTAAG 1 cut(s) 1525
MspR9I CCNGG 9 cut(s) 136, 509, 967, 1163, 1607, 1856, 1955, 1956, 2723
Mva1269I GAATGC 1 cut(s) 2975
MvaI CCWGG 6 cut(s) 136, 967, 1163, 1607, 1856, 2723
MvnI CGCG 2 cut(s) 561, 1367
NciI CCSGG 3 cut(s) 509, 1955, 1956
NlaIII CATG 8 cut(s) 302, 2248, 2285, 2555, 2794, 2905, 2935, 3079
NmeAIII GCCGAG 1 cut(s) 2962
NmuCI GTSAC 6 cut(s) 177, 548, 726, 2875, 2927, 3048
NspI RCATGY 1 cut(s) 2285
NspV TTCGAA 1 cut(s) 602
PagI TCATGA 1 cut(s) 2551
PaqCI CACCTGC 1 cut(s) 2552
PciSI GCTCTTC 1 cut(s) 1151
PcsI WCGNNNNNNNCGW 2 cut(s) 1623, 2356
PctI GAATGC 1 cut(s) 2975
PdmI GAANNNNTTC 2 cut(s) 683, 1046
PfeI GAWTC 5 cut(s) 970, 1459, 1630, 2046, 2770
PflMI CCANNNNNTGG 1 cut(s) 1471
PfoI TCCNGGA 1 cut(s) 1605
PinAI ACCGGT 1 cut(s) 2589
PleI GAGTC 5 cut(s) 807, 1237, 1967, 1997, 2482
PpsI GAGTC 5 cut(s) 807, 1237, 1967, 1997, 2482
Ppu21I YACGTR 1 cut(s) 172
PpuMI RGGWCCY 2 cut(s) 123, 1603
PshBI ATTAAT 1 cut(s) 957
Psp124BI GAGCTC 1 cut(s) 522
Psp5II RGGWCCY 2 cut(s) 123, 1603
Psp6I CCWGG 6 cut(s) 134, 965, 1161, 1605, 1854, 2721
PspEI GGTNACC 1 cut(s) 1055
PspFI CCCAGC 2 cut(s) 851, 3001
PspGI CCWGG 6 cut(s) 134, 965, 1161, 1605, 1854, 2721
PspPI GGNCC 8 cut(s) 123, 142, 861, 1023, 1174, 1521, 1603, 1715
PspPPI RGGWCCY 2 cut(s) 123, 1603
PstI CTGCAG 3 cut(s) 1729, 1939, 2086
PstNI CAGNNNCTG 1 cut(s) 2090
PsuI RGATCY 2 cut(s) 421, 500
RsaI GTAC 6 cut(s) 26, 174, 1242, 1375, 2846, 2900
RsaNI GTAC 6 cut(s) 25, 173, 1241, 1374, 2845, 2899
RseI CAYNNNNRTG 2 cut(s) 2120, 2550
SacI GAGCTC 1 cut(s) 522
SapI GCTCTTC 1 cut(s) 1151
SaqAI TTAA 9 cut(s) 18, 626, 813, 957, 1485, 1526, 1688, 1941, 2127
Sau96I GGNCC 8 cut(s) 123, 142, 861, 1023, 1174, 1521, 1603, 1715
ScaI AGTACT 1 cut(s) 1242
SchI GAGTC 5 cut(s) 808, 1238, 1968, 1997, 2482
ScrFI CCNGG 9 cut(s) 136, 509, 967, 1163, 1607, 1856, 1955, 1956, 2723
SduI GDGCHC 2 cut(s) 522, 791
SfaNI GCATC 2 cut(s) 2554, 2968
SfcI CTRYAG 3 cut(s) 1725, 1935, 2082
SfuI TTCGAA 1 cut(s) 602
SinI GGWCC 7 cut(s) 123, 142, 861, 1023, 1174, 1603, 1715
SmaI CCCGGG 1 cut(s) 1956
SmiMI CAYNNNNRTG 2 cut(s) 2120, 2550
SmlI CTYRAG 7 cut(s) 250, 865, 1018, 1226, 1525, 1621, 2406
SmoI CTYRAG 7 cut(s) 250, 865, 1018, 1226, 1525, 1621, 2406
SnaBI TACGTA 1 cut(s) 172
SsiI CCGC 7 cut(s) 75, 561, 1340, 1367, 1871, 2088, 2168
SspMI CTAG 7 cut(s) 803, 1182, 1406, 2391, 2727, 2867, 3032
SstI GAGCTC 1 cut(s) 522
StyD4I CCNGG 9 cut(s) 134, 507, 965, 1161, 1605, 1854, 1953, 1954, 2721
StyI CCWWGG 2 cut(s) 2782, 2989
TaaI ACNGT 9 cut(s) 177, 464, 683, 1670, 2122, 2303, 2344, 2386, 2818
TaiI ACGT 5 cut(s) 174, 387, 1511, 1542, 3050
TaqII GACCGA 1 cut(s) 2763
TatI WGTACW 2 cut(s) 1240, 2898
TauI GCSGC 4 cut(s) 564, 1370, 2090, 2171
TfiI GAWTC 5 cut(s) 970, 1459, 1630, 2046, 2770
Tru1I TTAA 9 cut(s) 18, 626, 813, 957, 1485, 1526, 1688, 1941, 2127
Tru9I TTAA 9 cut(s) 18, 626, 813, 957, 1485, 1526, 1688, 1941, 2127
TscAI CASTG 8 cut(s) 469, 735, 888, 1273, 2292, 2389, 2884, 2952
TseFI GTSAC 6 cut(s) 177, 548, 726, 2875, 2927, 3048
TseI GCWGC 9 cut(s) 308, 1092, 1530, 1727, 2081, 2084, 2171, 2688, 2763
Tsp45I GTSAC 6 cut(s) 177, 548, 726, 2875, 2927, 3048
TspDTI ATGAA 7 cut(s) 103, 315, 1169, 1425, 2540, 2657, 3041
TspGWI ACGGA 2 cut(s) 1894, 2123
TspMI CCCGGG 1 cut(s) 1954
TspRI CASTG 8 cut(s) 469, 735, 888, 1273, 2292, 2389, 2884, 2952
Van91I CCANNNNNTGG 1 cut(s) 1471
Vha464I CTTAAG 1 cut(s) 1525
VpaK11BI GGWCC 7 cut(s) 123, 142, 861, 1023, 1174, 1603, 1715
VspI ATTAAT 1 cut(s) 957
XagI CCTNNNNNAGG 1 cut(s) 3120
XbaI TCTAGA 1 cut(s) 2866
XceI RCATGY 1 cut(s) 2285
XmaI CCCGGG 1 cut(s) 1954
XmiI GTMKAC 1 cut(s) 2334
XmnI GAANNNNTTC 2 cut(s) 683, 1046
XspI CTAG 7 cut(s) 803, 1182, 1406, 2391, 2727, 2867, 3032
ZrmI AGTACT 1 cut(s) 1242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.