pycom01g19960

Embryo-specific protein 3, (ATS3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014997
Physical Location & Seq
Forward (+)
18453372 .. 18454642
1271 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 342 bp
ATGCTTATGGCAACCAGATTTATGCGCCGCGATTGGATCGATCCATCTTCGAGAACATTTGAACGGTGTTCTACAGACACATTTGAGATTTACGGCCCCTGTGCATACCAAATCTGCTATTTGTATGTCTACAGATCCGGACCGGATGGTTGGAAGCCTGAGAATGTGAAAATCTATGGCCACAATTCCAGGGCTGTTACATTTAGCTACAACACCTTCATTCCCAATGATCTTTGGTACGGCTTTAACTGGTGCAACACCGCCTCCTCTTCCGCCCGGAAATCTGCTCACAGATGGCCTCTGTATGCCGTAGCTGCGCTCTTTGTTTTTCTTATGTTGTAA

Protein Analysis

114

Amino Acids

13.31

Weight (kDa)

8.98

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATS3 PF06232 14 - 85 3.1e-31 Embryo-specific protein 3, (ATS3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62200
fragaria_vesca FvH4_7g28400
malus_domestica MD01G1187700.v1.1 MD07G1259800.v1.1
prunus_persica Prupe.2G284100_v2.0.a1
pyrus_communis pycom01g19960 pycom07g23320
rosa_chinensis RchiOBHm_Chr1g0375361
rosa_laevigata RLG00000026668
rosa_rugosa Rorug01G0390500.1 Rorug01G0390600
rosa_samantha Rh1AG401000 Rh1BG363300 Rh1CG376600 Rh1DG394100
rosa_wichuraiana Rw1G035600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 129
AccII CGCG 1 cut(s) 30
AccIII TCCGGA 1 cut(s) 137
AciI CCGC 3 cut(s) 28, 261, 273
AclWI GGATC 3 cut(s) 35, 44, 129
AcoI YGGCCR 1 cut(s) 178
AfaI GTAC 1 cut(s) 239
AgsI TTSAA 1 cut(s) 62
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 2 cut(s) 207, 314
AluI AGCT 2 cut(s) 207, 314
AlwI GGATC 3 cut(s) 35, 44, 129
Aor13HI TCCGGA 1 cut(s) 137
AoxI GGCC 3 cut(s) 94, 178, 296
ApeKI GCWGC 1 cut(s) 314
AspLEI GCGC 2 cut(s) 27, 319
AspS9I GGNCC 2 cut(s) 95, 140
AsuC2I CCSGG 1 cut(s) 277
AvaII GGWCC 1 cut(s) 140
BalI TGGCCA 1 cut(s) 180
BbvI GCAGC 1 cut(s) 301
BccI CCATC 3 cut(s) 52, 140, 288
BceAI ACGGC 3 cut(s) 109, 256, 293
BciT130I CCWGG 1 cut(s) 190
BcnI CCSGG 1 cut(s) 277
BfmI CTRYAG 2 cut(s) 72, 130
BisI GCNGC 2 cut(s) 28, 315
BlsI GCNGC 2 cut(s) 29, 316
Bme1390I CCNGG 2 cut(s) 190, 277
Bme18I GGWCC 1 cut(s) 140
BmgT120I GGNCC 2 cut(s) 95, 140
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 2 cut(s) 190, 277
BpuMI CCSGG 1 cut(s) 277
Bsa29I ATCGAT 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 189
BsaWI WCCGGW 2 cut(s) 137, 142
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bse1I ACTGG 1 cut(s) 254
BseAI TCCGGA 1 cut(s) 137
BseBI CCWGG 1 cut(s) 190
BseCI ATCGAT 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 1 cut(s) 151
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 254
BseRI GAGGAG 1 cut(s) 256
BseXI GCAGC 1 cut(s) 301
Bsh1236I CGCG 1 cut(s) 30
BshFI GGCC 3 cut(s) 96, 180, 298
BshVI ATCGAT 1 cut(s) 39
BsiSI CCGG 3 cut(s) 138, 143, 277
BsnI GGCC 3 cut(s) 96, 180, 298
Bsp13I TCCGGA 1 cut(s) 137
Bsp143I GATC 4 cut(s) 36, 40, 134, 229
BspACI CCGC 3 cut(s) 28, 261, 273
BspANI GGCC 3 cut(s) 96, 180, 298
BspCNI CTCAG 1 cut(s) 151
BspDI ATCGAT 1 cut(s) 39
BspEI TCCGGA 1 cut(s) 137
BspFNI CGCG 1 cut(s) 30
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 3 cut(s) 35, 44, 129
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 4 cut(s) 36, 40, 134, 229
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 1 cut(s) 66
Bst6I CTCTTC 1 cut(s) 274
BstDEI CTNAG 1 cut(s) 159
BstF5I GGATG 1 cut(s) 151
BstFNI CGCG 1 cut(s) 30
BstHHI GCGC 2 cut(s) 27, 319
BstKTI GATC 4 cut(s) 39, 43, 137, 232
BstMBI GATC 4 cut(s) 36, 40, 134, 229
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstNI CCWGG 1 cut(s) 190
BstSCI CCNGG 2 cut(s) 188, 275
BstSFI CTRYAG 2 cut(s) 72, 130
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 301
BstX2I RGATCY 1 cut(s) 134
BstYI RGATCY 1 cut(s) 134
Bsu15I ATCGAT 1 cut(s) 39
BsuRI GGCC 3 cut(s) 96, 180, 298
BsuTUI ATCGAT 1 cut(s) 39
BtsCI GGATG 1 cut(s) 151
CfoI GCGC 2 cut(s) 27, 319
Cfr13I GGNCC 2 cut(s) 95, 140
ClaI ATCGAT 1 cut(s) 39
CpoI CGGWCCG 1 cut(s) 140
Csp6I GTAC 1 cut(s) 238
CspI CGGWCCG 1 cut(s) 140
CviJI RGCY 8 cut(s) 96, 157, 180, 194, 207, 243, 298, 314
CviKI_1 RGCY 8 cut(s) 96, 157, 180, 194, 207, 243, 298, 314
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 1 cut(s) 159
DpnI GATC 4 cut(s) 38, 42, 136, 231
DpnII GATC 4 cut(s) 36, 40, 134, 229
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 274
EarI CTCTTC 1 cut(s) 274
EciI GGCGGA 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 188
FaiI YATR 7 cut(s) 8, 23, 106, 126, 177, 306, 335
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 2 cut(s) 28, 315
FokI GGATG 1 cut(s) 158
Fsp4HI GCNGC 2 cut(s) 28, 315
GlaI GCGC 2 cut(s) 26, 318
GluI GCNGC 2 cut(s) 28, 315
HaeIII GGCC 3 cut(s) 96, 180, 298
HapII CCGG 3 cut(s) 138, 143, 277
HhaI GCGC 2 cut(s) 27, 319
Hin6I GCGC 2 cut(s) 25, 317
HinP1I GCGC 2 cut(s) 25, 317
HpaII CCGG 3 cut(s) 138, 143, 277
Hpy166II GTNNAC 1 cut(s) 130
Hpy188III TCNNGA 2 cut(s) 51, 138
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 1 cut(s) 66
HpyCH4V TGCA 2 cut(s) 104, 255
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 1 cut(s) 159
HspAI GCGC 2 cut(s) 25, 317
Kpn2I TCCGGA 1 cut(s) 137
Kzo9I GATC 4 cut(s) 36, 40, 134, 229
LpnPI CCDG 9 cut(s) 28, 112, 151, 156, 171, 175, 202, 235, 290
Lsp1109I GCAGC 1 cut(s) 301
MaeIII GTNAC 1 cut(s) 196
MalI GATC 4 cut(s) 38, 42, 136, 231
MboI GATC 4 cut(s) 36, 40, 134, 229
MboII GAAGA 2 cut(s) 39, 261
MflI RGATCY 1 cut(s) 134
MlsI TGGCCA 1 cut(s) 180
MluCI AATT 1 cut(s) 184
MluNI TGGCCA 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 131
MnlI CCTC 3 cut(s) 274, 277, 309
Mox20I TGGCCA 1 cut(s) 180
MroI TCCGGA 1 cut(s) 137
MscI TGGCCA 1 cut(s) 180
MseI TTAA 1 cut(s) 246
Msp20I TGGCCA 1 cut(s) 180
MspI CCGG 3 cut(s) 138, 143, 277
MspR9I CCNGG 2 cut(s) 190, 277
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 1 cut(s) 314
NciI CCSGG 1 cut(s) 277
NdeII GATC 4 cut(s) 36, 40, 134, 229
NlaIV GGNNCC 1 cut(s) 97
PkrI GCNGC 2 cut(s) 29, 316
Psp6I CCWGG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 2 cut(s) 95, 140
PsuI RGATCY 1 cut(s) 134
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
Rsr2I CGGWCCG 1 cut(s) 140
RsrII CGGWCCG 1 cut(s) 140
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 2 cut(s) 28, 315
Sau3AI GATC 4 cut(s) 36, 40, 134, 229
Sau96I GGNCC 2 cut(s) 95, 140
ScrFI CCNGG 2 cut(s) 190, 277
SetI ASST 3 cut(s) 209, 218, 316
SfcI CTRYAG 2 cut(s) 72, 130
SinI GGWCC 1 cut(s) 140
Sse9I AATT 1 cut(s) 184
SsiI CCGC 3 cut(s) 28, 261, 273
StyD4I CCNGG 2 cut(s) 188, 275
TaaI ACNGT 1 cut(s) 66
TaqI TCGA 2 cut(s) 39, 50
TasI AATT 1 cut(s) 184
TauI GCSGC 1 cut(s) 30
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TseI GCWGC 1 cut(s) 314
TspDTI ATGAA 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 140
XmiI GTMKAC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.