pycom01g20640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0015498
Physical Location & Seq
Forward (+)
18985118 .. 18986129
1012 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g20640.3

Sequence Viewer

Length: 822 bp
ATGGAGGGGACCACAAACACATTTCTCTGCTTGGACTTGTCTGTAATAAAAACCAGCCTCAAATTTTGCAGTGGAAAATTAAAAAAAGAAAAAAGAAACACAGAAGAAAGGTACCCGCCAAAAATGGGGTGCTGCAGGATAAACACCTCCAACCTTCCAGCTCTCTGGAAATTCCCAACTCAAAACCAAAATCCCAGAACCAAAACCATCATCTCCTTCTCTTCCCCTTCTTGCTCTCAAACCGAAGATCGAAAATCGAAACCTTATACCGTAAACTTCAAGACTTTGAGTGCCTGCAAGCTTGGCATAGCCAGGTATCCTGACTTTGAATACAATGCTAAAGGTGGAGCGGGAACCGGGTCTGCCACAGAGGTCACGGAAAGCAACTCGAATGGCGATGTTTTGGTGTCGTTTGACATCGGAAGGCTCTATATCCCGCCATTGGAGAGTGCAACAACAAAGTTCTTGGGGTTGCCATTGCCGCCCTTTTTGACGATTGACATTGTCCCTGAGTTCTTCAGTGGGAGCATCAATCCAGAATCCGGCCAGGTTGATCTCGAATTCAAGGCGGAGTTTTGGTTTTCGGTTGGGAGTATATACAAGGCTCCTCCGCTGCTGGTGAGGACGGTATTGACATCCGAGGAATCGAAAGGAACGATGAGAAGTGGAAGAGGGGAGAGGTTGGATAAACAAGGAAAGTGCAGACTTGTTGGGGTGGCAACCGTTGATCCTATCGATGACTTTCTGATCAACTCCTTCCTTGGCCTTCCCACGGAATGCCTGGCCGACTTGAACGCTATTATTTCCTTCTCTAGCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

274

Amino Acids

29.96

Weight (kDa)

8.35

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62140
fragaria_vesca FvH4_7g28911 FvH4_7g28911
malus_domestica MD01G1195800.v1.1
prunus_persica Prupe.2G289100_v2.0.a1
pyrus_communis pycom01g20640 pycom07g24160
rosa_chinensis RchiOBHm_Chr1g0376581
rosa_laevigata RLG00000026596
rosa_roxburghii Rroxscaffold_4G00281970
rosa_rugosa Rorug01G0396500
rosa_samantha Rh1AG412100 Rh1BG372800 Rh1CG386700 Rh1DG403700
rosa_wichuraiana Rw1G036220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 111
AccB1I GGYRCC 1 cut(s) 111
AccBSI CCGCTC 1 cut(s) 350
AciI CCGC 6 cut(s) 116, 350, 437, 482, 569, 611
AclWI GGATC 1 cut(s) 722
AcoI YGGCCR 2 cut(s) 544, 783
AcsI RAATTY 3 cut(s) 62, 170, 560
AcuI CTGAAG 1 cut(s) 502
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 4 cut(s) 125, 442, 542, 772
AgsI TTSAA 4 cut(s) 280, 329, 565, 793
AjnI CCWGG 3 cut(s) 311, 546, 780
AluBI AGCT 3 cut(s) 161, 301, 816
AluI AGCT 3 cut(s) 161, 301, 816
AlwI GGATC 1 cut(s) 722
AoxI GGCC 3 cut(s) 544, 763, 783
ApeKI GCWGC 2 cut(s) 132, 613
ApoI RAATTY 3 cut(s) 62, 170, 560
Asp718I GGTACC 1 cut(s) 111
AspS9I GGNCC 1 cut(s) 9
AsuC2I CCSGG 1 cut(s) 358
AsuHPI GGTGA 1 cut(s) 631
AvaII GGWCC 1 cut(s) 9
BanI GGYRCC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 119, 600
BccI CCATC 1 cut(s) 215
BciT130I CCWGG 3 cut(s) 313, 548, 782
BciVI GTATCC 1 cut(s) 327
BclI TGATCA 1 cut(s) 747
BcnI CCSGG 1 cut(s) 358
BfaI CTAG 1 cut(s) 813
BfmI CTRYAG 1 cut(s) 133
BfuI GTATCC 1 cut(s) 327
BisI GCNGC 3 cut(s) 133, 482, 614
BlsI GCNGC 3 cut(s) 134, 483, 615
Bme1390I CCNGG 4 cut(s) 313, 358, 548, 782
Bme18I GGWCC 1 cut(s) 9
BmgT120I GGNCC 1 cut(s) 9
BmiI GGNNCC 4 cut(s) 10, 113, 355, 606
BmrFI CCNGG 4 cut(s) 313, 358, 548, 782
BmsI GCATC 1 cut(s) 537
BpuMI CCSGG 1 cut(s) 358
Bsa29I ATCGAT 1 cut(s) 735
BsaJI CCNNGG 3 cut(s) 639, 760, 771
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 442, 542, 772
Bse3DI GCAATG 1 cut(s) 476
BseBI CCWGG 3 cut(s) 313, 548, 782
BseCI ATCGAT 1 cut(s) 735
BseDI CCNNGG 3 cut(s) 639, 760, 771
BseGI GGATG 1 cut(s) 635
BseLI CCNNNNNNNGG 4 cut(s) 125, 442, 542, 772
BseMI GCAATG 1 cut(s) 476
BseMII CTCAG 1 cut(s) 501
BseRI GAGGAG 1 cut(s) 597
BseXI GCAGC 2 cut(s) 119, 600
BsgI GTGCAG 1 cut(s) 721
BshFI GGCC 3 cut(s) 546, 765, 785
BshNI GGYRCC 1 cut(s) 111
BshVI ATCGAT 1 cut(s) 735
BsiSI CCGG 2 cut(s) 357, 543
BslFI GGGAC 2 cut(s) 22, 491
BslI CCNNNNNNNGG 4 cut(s) 125, 442, 542, 772
BsmFI GGGAC 2 cut(s) 22, 491
BsmI GAATGC 1 cut(s) 782
BsnI GGCC 3 cut(s) 546, 765, 785
Bsp143I GATC 4 cut(s) 247, 553, 727, 747
BspACI CCGC 6 cut(s) 116, 350, 437, 482, 569, 611
BspANI GGCC 3 cut(s) 546, 765, 785
BspCNI CTCAG 1 cut(s) 502
BspDI ATCGAT 1 cut(s) 735
BspLI GGNNCC 4 cut(s) 10, 113, 355, 606
BspMAI CTGCAG 1 cut(s) 137
BspPI GGATC 1 cut(s) 722
BspT107I GGYRCC 1 cut(s) 111
BsrBI CCGCTC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 476
BssECI CCNNGG 3 cut(s) 639, 760, 771
BssMI GATC 4 cut(s) 247, 553, 727, 747
BssT1I CCWWGG 1 cut(s) 760
Bst2UI CCWGG 3 cut(s) 313, 548, 782
Bst4CI ACNGT 3 cut(s) 271, 628, 724
Bst6I CTCTTC 2 cut(s) 226, 664
BstC8I GCNNGC 2 cut(s) 295, 299
BstDEI CTNAG 2 cut(s) 510, 819
BstDSI CCRYGG 1 cut(s) 771
BstF5I GGATG 1 cut(s) 635
BstKTI GATC 4 cut(s) 250, 556, 730, 750
BstMBI GATC 4 cut(s) 247, 553, 727, 747
BstMWI GCNNNNNNNGC 2 cut(s) 303, 481
BstNI CCWGG 3 cut(s) 313, 548, 782
BstSCI CCNGG 4 cut(s) 311, 356, 546, 780
BstSFI CTRYAG 1 cut(s) 133
BstV1I GCAGC 2 cut(s) 119, 600
BstXI CCANNNNNNTGG 1 cut(s) 165
Bsu15I ATCGAT 1 cut(s) 735
BsuI GTATCC 1 cut(s) 327
BsuRI GGCC 3 cut(s) 546, 765, 785
BsuTUI ATCGAT 1 cut(s) 735
BtgI CCRYGG 1 cut(s) 771
BtgZI GCGATG 1 cut(s) 411
BtsCI GGATG 1 cut(s) 635
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 2 cut(s) 76, 526
Cac8I GCNNGC 2 cut(s) 295, 299
Cfr13I GGNCC 1 cut(s) 9
ClaI ATCGAT 1 cut(s) 735
Csp6I GTAC 1 cut(s) 112
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 2 cut(s) 510, 819
DpnI GATC 4 cut(s) 249, 555, 729, 749
DpnII GATC 4 cut(s) 247, 553, 727, 747
EaeI YGGCCR 2 cut(s) 544, 783
Eam1104I CTCTTC 2 cut(s) 226, 664
EarI CTCTTC 2 cut(s) 226, 664
EciI GGCGGA 1 cut(s) 584
Eco130I CCWWGG 1 cut(s) 760
Eco47I GGWCC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 502
EcoRI GAATTC 1 cut(s) 560
EcoRII CCWGG 3 cut(s) 311, 546, 780
EcoT14I CCWWGG 1 cut(s) 760
ErhI CCWWGG 1 cut(s) 760
FaiI YATR 5 cut(s) 267, 308, 432, 596, 598
FaqI GGGAC 2 cut(s) 22, 491
FauI CCCGC 3 cut(s) 123, 343, 444
FbaI TGATCA 1 cut(s) 747
Fnu4HI GCNGC 3 cut(s) 133, 482, 614
FokI GGATG 1 cut(s) 622
Fsp4HI GCNGC 3 cut(s) 133, 482, 614
FspBI CTAG 1 cut(s) 813
GluI GCNGC 3 cut(s) 133, 482, 614
HaeIII GGCC 3 cut(s) 546, 765, 785
HapII CCGG 2 cut(s) 357, 543
HindIII AAGCTT 1 cut(s) 299
HinfI GANTC 2 cut(s) 539, 644
HpaII CCGG 2 cut(s) 357, 543
HphI GGTGA 1 cut(s) 631
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 3 cut(s) 422, 640, 747
Hpy188III TCNNGA 5 cut(s) 166, 280, 320, 536, 557
Hpy8I GTNNAC 1 cut(s) 274
HpyAV CCTTC 7 cut(s) 164, 226, 237, 417, 766, 776, 817
HpyCH4III ACNGT 3 cut(s) 271, 628, 724
HpyCH4V TGCA 5 cut(s) 69, 135, 297, 452, 702
HpyF10VI GCNNNNNNNGC 2 cut(s) 303, 481
HpyF3I CTNAG 2 cut(s) 510, 819
KpnI GGTACC 1 cut(s) 115
Ksp22I TGATCA 1 cut(s) 747
Kzo9I GATC 4 cut(s) 247, 553, 727, 747
LmnI GCTCC 3 cut(s) 347, 525, 610
Lsp1109I GCAGC 2 cut(s) 119, 600
LweI GCATC 1 cut(s) 537
MaeI CTAG 1 cut(s) 813
MaeIII GTNAC 1 cut(s) 373
MalI GATC 4 cut(s) 249, 555, 729, 749
MbiI CCGCTC 1 cut(s) 350
MboI GATC 4 cut(s) 247, 553, 727, 747
MboII GAAGA 5 cut(s) 116, 213, 257, 508, 681
MluCI AATT 4 cut(s) 62, 77, 170, 560
MmeI TCCRAC 2 cut(s) 174, 663
MnlI CCTC 8 cut(s) 68, 157, 364, 615, 618, 634, 665, 672
MseI TTAA 1 cut(s) 80
MspA1I CMGCKG 1 cut(s) 613
MspI CCGG 2 cut(s) 357, 543
MspR9I CCNGG 4 cut(s) 313, 358, 548, 782
Mva1269I GAATGC 1 cut(s) 782
MvaI CCWGG 3 cut(s) 313, 548, 782
MwoI GCNNNNNNNGC 2 cut(s) 303, 481
NciI CCSGG 1 cut(s) 358
NdeII GATC 4 cut(s) 247, 553, 727, 747
NlaIV GGNNCC 4 cut(s) 10, 113, 355, 606
NmuCI GTSAC 1 cut(s) 373
PcsI WCGNNNNNNNCGW 1 cut(s) 653
PctI GAATGC 1 cut(s) 782
PfeI GAWTC 2 cut(s) 539, 644
PflFI GACNNNGTC 1 cut(s) 503
PkrI GCNGC 3 cut(s) 134, 483, 615
Psp6I CCWGG 3 cut(s) 311, 546, 780
PspGI CCWGG 3 cut(s) 311, 546, 780
PspN4I GGNNCC 4 cut(s) 10, 113, 355, 606
PspPI GGNCC 1 cut(s) 9
PstI CTGCAG 1 cut(s) 137
PsyI GACNNNGTC 1 cut(s) 503
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SaqAI TTAA 1 cut(s) 80
SatI GCNGC 3 cut(s) 133, 482, 614
Sau3AI GATC 4 cut(s) 247, 553, 727, 747
Sau96I GGNCC 1 cut(s) 9
ScrFI CCNGG 4 cut(s) 313, 358, 548, 782
SfaNI GCATC 1 cut(s) 537
SfcI CTRYAG 1 cut(s) 133
SinI GGWCC 1 cut(s) 9
Sse9I AATT 4 cut(s) 62, 77, 170, 560
SsiI CCGC 6 cut(s) 116, 350, 437, 482, 569, 611
SspMI CTAG 1 cut(s) 813
StyD4I CCNGG 4 cut(s) 311, 356, 546, 780
StyI CCWWGG 1 cut(s) 760
TaaI ACNGT 3 cut(s) 271, 628, 724
TaqI TCGA 6 cut(s) 250, 257, 389, 558, 647, 735
TasI AATT 4 cut(s) 62, 77, 170, 560
TauI GCSGC 1 cut(s) 484
TfiI GAWTC 2 cut(s) 539, 644
Tru1I TTAA 1 cut(s) 80
Tru9I TTAA 1 cut(s) 80
TscAI CASTG 2 cut(s) 76, 526
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 2 cut(s) 132, 613
Tsp45I GTSAC 1 cut(s) 373
TspGWI ACGGA 2 cut(s) 392, 788
TspRI CASTG 2 cut(s) 76, 526
Tth111I GACNNNGTC 1 cut(s) 503
VpaK11BI GGWCC 1 cut(s) 9
XapI RAATTY 3 cut(s) 62, 170, 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 778
XspI CTAG 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.