pycom01g20850

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0011369
Physical Location & Seq
Forward (+)
19155165 .. 19156751
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 978 bp
ATGGATATTTTGATTGCGTACAGCTCCTACTTGATCTATCAAGCAAATGTGTCTGCTGTGACGTTTCATTACGGGACTTCTATGGATTTGATAGGAGCTGGAAGTTCTCCACTGCATTATGCTGCTTGTGGGGGTAACTTAAAGTGCTGTCAGATCCTCCTTGCAAGAGGTGCCAGCAAGACGACTTTAAACTGCACTGGGTGGCTTCCAGTTGATGTTGCCCGAATGTGGGGGCGCCACTGGCTTGAACCACTTCTAGCACCCAATTCTGACTCGACAATACCAGCGTTCCCCCTATCTAATTACCTATCCTTGCCTCTCATGAGCATACTGAATATAGCGAGGGAATGTGGATTTAAGTCCGTTACAACATCAACCGACGACACAGATACTTGTGCTGTCTGTCTGGAAAGACCATGTTCGGTAGCTGCAGAAGGCTGTAAGCATGAGCTTTGTGTAAGATGCGCACTCTATCTTTGCTCAACGAGCAACATACCTTCGGAAACGGTGGGCCCACCTGGGTCCATTCCATGCCCGCTTTGTAGGCACGGTATCATCTCCTTTGTCAAATTGCCTGGCTCCCCAGCAAAGGAAAATAGATTACAGATGTCACTTGGCCTTTGTACCCCTTGTATACTTCACCCTCGTGACCCAGAGCGTCTTTCTCCAGCTTGTGCACCAGAGATCCGAAAGAACCGTGTGGCTTCAGTTTCTTCGGATATGCTCTGTCCAGTTACATGCAGTCCATTTCCTTCTGTCACCATTCCTTTGTGCACGTGCAATGATGGACCATGCCCCTCATTTGAAACCCGGGAGGCAGAAACCCAAGATGAATTGCCCAGGCGGCCCCAAGCAACATCAGCAGACCAGGACAAGATGGAAGGGCCGAGACTTGGAAAAACAACATGTTCAAACATGTTTTGGAGCAGAAGGAGCTGCAGTAGGGAGCATCAGTGCAATGCCGAAATAAATGCTTAA

Protein Analysis

326

Amino Acids

35.21

Weight (kDa)

6.63

Isoelectric Point (pI)

56.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RING_XB3-XBAT31 PF24921 131 - 186 1.8e-17 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 466
AccB1I GGYRCC 2 cut(s) 170, 234
AccI GTMKAC 1 cut(s) 634
AciI CCGC 2 cut(s) 536, 844
AclWI GGATC 2 cut(s) 148, 679
AcuI CTGAAG 1 cut(s) 690
AcvI CACGTG 1 cut(s) 777
AcyI GRCGYC 1 cut(s) 235
AdeI CACNNNGTG 1 cut(s) 201
AfaI GTAC 2 cut(s) 20, 625
AfiI CCNNNNNNNGG 4 cut(s) 228, 229, 589, 893
AflIII ACRYGT 2 cut(s) 905, 915
AgsI TTSAA 3 cut(s) 248, 806, 912
AjnI CCWGG 4 cut(s) 517, 574, 839, 867
AleI CACNNNNGTG 1 cut(s) 645
AluBI AGCT 6 cut(s) 24, 98, 428, 451, 671, 936
AluI AGCT 6 cut(s) 24, 98, 428, 451, 671, 936
Alw21I GWGCWC 2 cut(s) 679, 776
Alw26I GTCTC 1 cut(s) 883
Alw44I GTGCAC 2 cut(s) 675, 772
AlwI GGATC 2 cut(s) 148, 679
Ama87I CYCGRG 1 cut(s) 810
AoxI GGCC 4 cut(s) 511, 616, 845, 884
ApaI GGGCCC 1 cut(s) 515
ApaLI GTGCAC 2 cut(s) 675, 772
ApeKI GCWGC 3 cut(s) 122, 428, 936
Asp700I GAANNNNTTC 1 cut(s) 252
AspLEI GCGC 2 cut(s) 237, 467
AspS9I GGNCC 6 cut(s) 511, 512, 522, 788, 846, 884
AsuC2I CCSGG 2 cut(s) 811, 812
AsuHPI GGTGA 2 cut(s) 632, 751
AvaI CYCGRG 1 cut(s) 810
AvaII GGWCC 2 cut(s) 522, 788
BaeGI GKGCMC 3 cut(s) 515, 679, 776
BanI GGYRCC 2 cut(s) 170, 234
BanII GRGCYC 1 cut(s) 515
BauI CACGAG 1 cut(s) 645
BbrPI CACGTG 1 cut(s) 777
Bbv12I GWGCWC 2 cut(s) 679, 776
BbvI GCAGC 3 cut(s) 109, 415, 923
BccI CCATC 2 cut(s) 779, 871
BcgI CGANNNNNNTGC 1 cut(s) 953
BciT130I CCWGG 4 cut(s) 519, 576, 841, 869
BcnI CCSGG 2 cut(s) 811, 812
BcoDI GTCTC 1 cut(s) 883
BfaI CTAG 1 cut(s) 257
BfmI CTRYAG 2 cut(s) 429, 937
BfoI RGCGCY 1 cut(s) 238
BglI GCCNNNNNGGC 1 cut(s) 844
BisI GCNGC 4 cut(s) 123, 429, 845, 937
BlsI GCNGC 4 cut(s) 124, 430, 846, 938
Bme1390I CCNGG 6 cut(s) 519, 576, 811, 812, 841, 869
Bme18I GGWCC 2 cut(s) 522, 788
BmeT110I CYCGRG 1 cut(s) 810
BmgT120I GGNCC 6 cut(s) 511, 512, 522, 788, 846, 884
BmiI GGNNCC 6 cut(s) 172, 236, 513, 523, 580, 848
BmrFI CCNGG 6 cut(s) 519, 576, 811, 812, 841, 869
BmrI ACTGGG 1 cut(s) 207
BmsI GCATC 2 cut(s) 452, 958
BmuI ACTGGG 1 cut(s) 207
BpmI CTGGAG 1 cut(s) 651
BpuMI CCSGG 2 cut(s) 811, 812
BsaAI YACGTR 1 cut(s) 777
BsaHI GRCGYC 1 cut(s) 235
BsaJI CCNNGG 3 cut(s) 518, 810, 839
BsaXI ACNNNNNCTCC 4 cut(s) 925, 938, 955, 968
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 229, 589, 893
Bse1I ACTGG 4 cut(s) 202, 209, 245, 731
Bse3DI GCAATG 2 cut(s) 787, 964
BseBI CCWGG 4 cut(s) 519, 576, 841, 869
BseDI CCNNGG 3 cut(s) 518, 810, 839
BseLI CCNNNNNNNGG 4 cut(s) 228, 229, 589, 893
BseMI GCAATG 2 cut(s) 787, 964
BseNI ACTGG 4 cut(s) 202, 209, 245, 731
BseSI GKGCMC 3 cut(s) 515, 679, 776
BseXI GCAGC 3 cut(s) 109, 415, 923
BseYI CCCAGC 1 cut(s) 583
BsgI GTGCAG 1 cut(s) 178
BshFI GGCC 4 cut(s) 513, 618, 847, 886
BshNI GGYRCC 2 cut(s) 170, 234
BsiHKAI GWGCWC 2 cut(s) 679, 776
BsiHKCI CYCGRG 1 cut(s) 810
BsiSI CCGG 1 cut(s) 811
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 4 cut(s) 228, 229, 589, 893
BsmAI GTCTC 1 cut(s) 883
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 4 cut(s) 513, 618, 847, 886
BsoBI CYCGRG 1 cut(s) 810
Bsp120I GGGCCC 1 cut(s) 511
Bsp1286I GDGCHC 3 cut(s) 515, 679, 776
Bsp143I GATC 3 cut(s) 33, 153, 684
BspACI CCGC 2 cut(s) 536, 844
BspANI GGCC 4 cut(s) 513, 618, 847, 886
BspHI TCATGA 1 cut(s) 321
BspLI GGNNCC 6 cut(s) 172, 236, 513, 523, 580, 848
BspMAI CTGCAG 2 cut(s) 433, 941
BspPI GGATC 2 cut(s) 148, 679
BspT107I GGYRCC 2 cut(s) 170, 234
BsrDI GCAATG 2 cut(s) 787, 964
BsrI ACTGG 4 cut(s) 202, 209, 245, 731
BssECI CCNNGG 3 cut(s) 518, 810, 839
BssMI GATC 3 cut(s) 33, 153, 684
BssNAI GTATAC 1 cut(s) 635
BssNI GRCGYC 1 cut(s) 235
BssSI CACGAG 1 cut(s) 645
Bst1107I GTATAC 1 cut(s) 635
Bst2BI CACGAG 1 cut(s) 645
Bst2UI CCWGG 4 cut(s) 519, 576, 841, 869
Bst4CI ACNGT 3 cut(s) 508, 551, 698
BstACI GRCGYC 1 cut(s) 235
BstAPI GCANNNNNTGC 1 cut(s) 170
BstBAI YACGTR 1 cut(s) 777
BstC8I GCNNGC 2 cut(s) 175, 536
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 2 cut(s) 237, 467
BstKTI GATC 3 cut(s) 36, 156, 687
BstMAI GTCTC 1 cut(s) 883
BstMBI GATC 3 cut(s) 33, 153, 684
BstMWI GCNNNNNNNGC 7 cut(s) 170, 241, 486, 544, 844, 860, 933
BstNI CCWGG 4 cut(s) 519, 576, 841, 869
BstNSI RCATGY 3 cut(s) 741, 909, 919
BstSCI CCNGG 6 cut(s) 517, 574, 809, 810, 839, 867
BstSFI CTRYAG 2 cut(s) 429, 937
BstSLI GKGCMC 3 cut(s) 515, 679, 776
BstV1I GCAGC 3 cut(s) 109, 415, 923
BstX2I RGATCY 2 cut(s) 153, 684
BstYI RGATCY 2 cut(s) 153, 684
BstZ17I GTATAC 1 cut(s) 635
BsuRI GGCC 4 cut(s) 513, 618, 847, 886
BtsI GCAGTG 1 cut(s) 110
BtsIMutI CAGTG 4 cut(s) 110, 195, 238, 959
Cac8I GCNNGC 2 cut(s) 175, 536
CciI TCATGA 1 cut(s) 321
CfoI GCGC 2 cut(s) 237, 467
Cfr13I GGNCC 6 cut(s) 511, 512, 522, 788, 846, 884
Cfr9I CCCGGG 1 cut(s) 810
CseI GACGC 1 cut(s) 647
Csp6I GTAC 2 cut(s) 19, 624
CviAII CATG 8 cut(s) 322, 417, 446, 531, 738, 792, 906, 916
CviQI GTAC 2 cut(s) 19, 624
DinI GGCGCC 1 cut(s) 236
DpnI GATC 3 cut(s) 35, 155, 686
DpnII GATC 3 cut(s) 33, 153, 684
DraI TTTAAA 1 cut(s) 189
DraIII CACNNNGTG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 515
Eco47I GGWCC 2 cut(s) 522, 788
Eco57I CTGAAG 1 cut(s) 690
Eco72I CACGTG 1 cut(s) 777
Eco88I CYCGRG 1 cut(s) 810
EcoRII CCWGG 4 cut(s) 517, 574, 839, 867
EcoT38I GRGCYC 1 cut(s) 515
EgeI GGCGCC 1 cut(s) 236
EheI GGCGCC 1 cut(s) 236
FaeI CATG 8 cut(s) 325, 420, 449, 534, 741, 795, 909, 919
FaqI GGGAC 1 cut(s) 88
FatI CATG 8 cut(s) 321, 416, 445, 530, 737, 791, 905, 915
FauI CCCGC 1 cut(s) 543
FblI GTMKAC 1 cut(s) 634
Fnu4HI GCNGC 4 cut(s) 123, 429, 845, 937
FriOI GRGCYC 1 cut(s) 515
Fsp4HI GCNGC 4 cut(s) 123, 429, 845, 937
FspAI RTGCGCAY 1 cut(s) 466
FspBI CTAG 1 cut(s) 257
FspI TGCGCA 1 cut(s) 466
GlaI GCGC 2 cut(s) 236, 466
GluI GCNGC 4 cut(s) 123, 429, 845, 937
GsaI CCCAGC 1 cut(s) 587
GsuI CTGGAG 1 cut(s) 651
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 4 cut(s) 513, 618, 847, 886
HapII CCGG 1 cut(s) 811
HgaI GACGC 1 cut(s) 647
HhaI GCGC 2 cut(s) 237, 467
Hin1I GRCGYC 1 cut(s) 235
Hin1II CATG 8 cut(s) 325, 420, 449, 534, 741, 795, 909, 919
Hin6I GCGC 2 cut(s) 235, 465
HinP1I GCGC 2 cut(s) 235, 465
HinfI GANTC 1 cut(s) 272
HpaII CCGG 1 cut(s) 811
HphI GGTGA 2 cut(s) 632, 751
Hpy166II GTNNAC 3 cut(s) 635, 677, 774
Hpy188I TCNGA 5 cut(s) 153, 271, 502, 689, 718
Hpy188III TCNNGA 3 cut(s) 322, 407, 647
Hpy8I GTNNAC 3 cut(s) 635, 677, 774
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 5 cut(s) 428, 507, 762, 875, 924
HpyCH4III ACNGT 3 cut(s) 508, 551, 698
HpyCH4IV ACGT 2 cut(s) 62, 776
HpyF10VI GCNNNNNNNGC 7 cut(s) 170, 241, 486, 544, 844, 860, 933
HpySE526I ACGT 2 cut(s) 62, 776
Hsp92I GRCGYC 1 cut(s) 235
Hsp92II CATG 8 cut(s) 325, 420, 449, 534, 741, 795, 909, 919
HspAI GCGC 2 cut(s) 235, 465
KasI GGCGCC 1 cut(s) 234
Kzo9I GATC 3 cut(s) 33, 153, 684
LmnI GCTCC 6 cut(s) 29, 95, 584, 924, 933, 946
Lsp1109I GCAGC 3 cut(s) 109, 415, 923
LweI GCATC 2 cut(s) 452, 958
MaeI CTAG 1 cut(s) 257
MaeII ACGT 2 cut(s) 62, 776
MaeIII GTNAC 7 cut(s) 58, 134, 364, 609, 647, 733, 757
MalI GATC 3 cut(s) 35, 155, 686
MboI GATC 3 cut(s) 33, 153, 684
MboII GAAGA 1 cut(s) 705
MflI RGATCY 2 cut(s) 153, 684
MhlI GDGCHC 3 cut(s) 515, 679, 776
MluCI AATT 4 cut(s) 265, 301, 569, 833
Mly113I GGCGCC 1 cut(s) 235
MlyI GAGTC 1 cut(s) 266
MnlI CCTC 7 cut(s) 161, 167, 327, 336, 654, 808, 808
MroXI GAANNNNTTC 1 cut(s) 252
MseI TTAA 4 cut(s) 140, 188, 357, 976
MslI CAYNNNNRTG 1 cut(s) 645
MspI CCGG 1 cut(s) 811
MspR9I CCNGG 6 cut(s) 519, 576, 811, 812, 841, 869
MvaI CCWGG 4 cut(s) 519, 576, 841, 869
MwoI GCNNNNNNNGC 7 cut(s) 170, 241, 486, 544, 844, 860, 933
NarI GGCGCC 1 cut(s) 235
NciI CCSGG 2 cut(s) 811, 812
NdeII GATC 3 cut(s) 33, 153, 684
NlaIII CATG 8 cut(s) 325, 420, 449, 534, 741, 795, 909, 919
NlaIV GGNNCC 6 cut(s) 172, 236, 513, 523, 580, 848
NmeAIII GCCGAG 1 cut(s) 912
NmuCI GTSAC 4 cut(s) 58, 609, 647, 757
NsbI TGCGCA 1 cut(s) 466
NspI RCATGY 3 cut(s) 741, 909, 919
OliI CACNNNNGTG 1 cut(s) 645
PagI TCATGA 1 cut(s) 321
PciI ACATGT 2 cut(s) 905, 915
PdmI GAANNNNTTC 1 cut(s) 252
PkrI GCNGC 4 cut(s) 124, 430, 846, 938
PleI GAGTC 1 cut(s) 266
PluTI GGCGCC 1 cut(s) 238
PmaCI CACGTG 1 cut(s) 777
PmlI CACGTG 1 cut(s) 777
PpsI GAGTC 1 cut(s) 266
Ppu21I YACGTR 1 cut(s) 777
PscI ACATGT 2 cut(s) 905, 915
Psp6I CCWGG 4 cut(s) 517, 574, 839, 867
PspCI CACGTG 1 cut(s) 777
PspFI CCCAGC 1 cut(s) 583
PspGI CCWGG 4 cut(s) 517, 574, 839, 867
PspN4I GGNNCC 6 cut(s) 172, 236, 513, 523, 580, 848
PspOMI GGGCCC 1 cut(s) 511
PspPI GGNCC 6 cut(s) 511, 512, 522, 788, 846, 884
PstI CTGCAG 2 cut(s) 433, 941
PsuI RGATCY 2 cut(s) 153, 684
RsaI GTAC 2 cut(s) 20, 625
RsaNI GTAC 2 cut(s) 19, 624
RseI CAYNNNNRTG 1 cut(s) 645
SaqAI TTAA 4 cut(s) 140, 188, 357, 976
SatI GCNGC 4 cut(s) 123, 429, 845, 937
Sau3AI GATC 3 cut(s) 33, 153, 684
Sau96I GGNCC 6 cut(s) 511, 512, 522, 788, 846, 884
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 6 cut(s) 519, 576, 811, 812, 841, 869
SduI GDGCHC 3 cut(s) 515, 679, 776
SfaNI GCATC 2 cut(s) 452, 958
SfcI CTRYAG 2 cut(s) 429, 937
SfoI GGCGCC 1 cut(s) 236
SinI GGWCC 2 cut(s) 522, 788
SmaI CCCGGG 1 cut(s) 812
SmiMI CAYNNNNRTG 1 cut(s) 645
Sse9I AATT 4 cut(s) 265, 301, 569, 833
SsiI CCGC 2 cut(s) 536, 844
SspDI GGCGCC 1 cut(s) 234
SspMI CTAG 1 cut(s) 257
StyD4I CCNGG 6 cut(s) 517, 574, 809, 810, 839, 867
TaaI ACNGT 3 cut(s) 508, 551, 698
TaiI ACGT 2 cut(s) 65, 779
TaqI TCGA 1 cut(s) 275
TasI AATT 4 cut(s) 265, 301, 569, 833
TauI GCSGC 1 cut(s) 847
Tru1I TTAA 4 cut(s) 140, 188, 357, 976
Tru9I TTAA 4 cut(s) 140, 188, 357, 976
TscAI CASTG 4 cut(s) 117, 202, 245, 959
TseFI GTSAC 4 cut(s) 58, 609, 647, 757
TseI GCWGC 3 cut(s) 122, 428, 936
Tsp45I GTSAC 4 cut(s) 58, 609, 647, 757
TspDTI ATGAA 2 cut(s) 56, 846
TspGWI ACGGA 1 cut(s) 352
TspMI CCCGGG 1 cut(s) 810
TspRI CASTG 4 cut(s) 117, 202, 245, 959
VneI GTGCAC 2 cut(s) 675, 772
VpaK11BI GGWCC 2 cut(s) 522, 788
XceI RCATGY 3 cut(s) 741, 909, 919
XmaI CCCGGG 1 cut(s) 810
XmiI GTMKAC 1 cut(s) 634
XmnI GAANNNNTTC 1 cut(s) 252
XspI CTAG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.