Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0011400

Physical Location & Seq

Genomic Coordinates Reverse (-)

19379829 .. 19391629

Gene Structure 11801 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4158 bp

ATGATTTCTGATTATTCGAATCCAAGCTGGGAAATGCAAAATCCTGTTATTTTATTCAGATTGCCGCATTTGCCCTCGTCGCGACTCTTACATCATTCGTCATTTCTCACACTCATCATTACGCACACAAATCTCGGCAAAGCGAGAAGACCGAGAGCGAGAAGACTGAGAACGAGAGAGTGGAGTGGCTACAGCAGCAGCAGAAGTTGCAGTGGTGGTAGGGTGAGGGAAATGCCTTGCTCTGCAATCGGCGGTGCTGGCGGAGGCGGTGGGGACAGTAATGGTGCTTTGAGCAATTTCAAGCTCAACGAGTCCACGTTCTTGGCCTCCCTCATGCCGAAGAAAGAGATTGGCGCCGATCGATTCATCGATGCGCATCCTAACTACGACGGCCGCGGCGCCCTCATCGCTATCTTCGATTCTGGAGTGGATCCAGCTGCTTCTGGATTACAAGTTACGTCAGACGGAAAGCCGAAAGTCCTAGATGTTCTTGATTGCACCGGGAGTGGTGATGTTGATACTTCGAAGGTAGTCAAGGCCGATGACGATGGTTGTATTCGTGGAGCTTCAGGAGCATCTCTGTTTGTCAACTCTTCATGGAAAAATCCTTCCGGTGAATGGCATGTGGGCTATAAATTGGTATATGAGCTATTTACAAATACGTTGACTTCTCGCTTGAAGAAAGAAAAAAGGAAAAAATGGGATGAACAAAACCAAGAAGAAATTGCCAAGGCTGTTAAACATCTTCAAGAGTTTGATCAGAAACACAGCAGATTGGACGATGTTAACTTGAAAAGAGCTCGCGAGGACCTGCAAAATAGAGTTGATTACCTACAAAAGCAAGCTGATAGCTATGATGACAAAGGGCCTGTCATAGATGCTGTTGTATGGCATGATGGAGAAGTATGGAGGGTTGCCCTTGACACACAGACTCTTGAGGATGACCCAGATTGTGGAAAACTTGCAAACTTTGTGCCCCTAACTAATTATAGGATAGAACGAAAATATGGTGTTTTTAGCAAATTAGATGCCTGTACATTTGTTGCTAATGTGTACGATGAAGGGAATATTCTAAGTATCGTAACAGATTGCCATCCACATGGCACTCATGTTGCTGGTATCGCTACTGCTTTCCACCCGAAGGAACCCCTGTTGAATGGAGTTGCACCTGGAGCACAAATAATATCTTGTAAGATTGGAGACTCACGTTTAGGTTTAATGGAGACTGGAACTGGGTTGACTCGAGCCCTGATTGCTGCTGTGGAGCATAAATGTGATTTGATCAACATGAGTTATGGAGAAGCTACACTGCTGCCAGACTATGGGCGTTTTGTTGACCTCGTTAATGAAGCTGTGAACAAGCACCATCTGATATTTGTGAGTAGTGCTGGCAATAATGGGCCAGCATTGAGCACTGTTGGAGCACCTGGTGGTACCACATCAAGCATTATAGGAGTTGGTGCATATGTCTCTCCTGCAATGGCTGCTGGTGCTCATTGTGTAGTTGAAGCTCCAGGTGAAGGGCTTGAGTACACTTGGTCTAGCCGTGGGCCAACCAGTGATGGAGCTCTTGGTGTCTGTATAAGTGCTCCTGGTGCAGCTGTTGCTCCTGTTCCTACATGGACTCTTCAGCGACGCATGCTCATGAATGGAACATCAATGTCATCTCCATCTGCTTGTGGGGGAATTGCATTGCTTGTTAGTGCAATGAAGGCTGAGGGTATTCCTGTGAGTCCATACAGTGTAAGGAAGGCTCTTGAGAATACATCTGTTCCTATAGGCAGTTTGCCTGAAGATAAACTATCAACTGGACAAGGGCTTATGCAAGTTGACAAAGCACATGAATACCTAAGGCAGAGCCGGGATGTACCAAGTGTTTGGTATCAGATAAAGATAAACCAATCTGGTAAAACAACTCCTACATCACGAGGAATTTACTTAAGGGAGGCTAGTGCTTTCCAACAATCTACCGAGTGGACAGTGCAAGTTGAACCAAAATTTCATGAAGGTGCTAGTAATTTGGAAGATTTGGTTCCTTTTGAAGAGTGTATTGAGCTACATTCCAGTGAGAAGGCAGTTTTGAGGGCTCCTGATTTTCTCCTTCTTACCCATAATGGGCGTAGCTTCAACATAGTAGTGGATCCCACCAATGTTAGTGAAGGTCTGCACTATTTTGAACTGTATGGTGTTGATTGTAAAGCACCATGGCGCGGCCCTCTTTTCAGAATCCCTGTTACCATAACAAAGCCTATAGCTGTGATAAGTCGACCTCCACTGTTGTCATTCTCGAGGATGTCATTTTTGCCAGGCCACATAGAAAGGAGATTTATAGAAGTACCTCTTGGTGCTACATGGGTTGAAGCAACTATGCGAACATCAGGATTTGATACATCAAGAAGATTTTTTATTGACTCTGTTCAGCTATGTCCCCTGCAAAGGCCTCTTAAATGGGAGAGTGTTGTAACATTCTCATCTCCTGCTGCCAAAAGCTTTTCATTTCCTGTTGTGGGTGGTCAAACAATGGAATTAGCTATTGCTCAATTTTGGTCTAGTGGCATAGGAAGCCATGAAACAACTATTGTGGATTTTGAGATTGTGTTTCATGGAATTAGCATTAATAAAGATGAAGTAGTGCTTGATGGAAGTGAAGGGCCAGCCAGAATTGAAGCTGAAGCTCTACTGGCTTCTGAGACACTTGCACCGGTTGCTATTCTAAACAAGATAAGAATTCCATACCGGCCGGTTGAGTCTAAACTTTGTTCACTTTCAACAGATCGTGACAAACTACCCTCAGAAAAACGTATTATGGCACTTACTTTAACCTACAAGATCAAATTGGAAGACGGAGCTGAAGTAAAGCCTCAAGTTCCATTACTTAACAATCGCGTATATGACACCAAATTTGAGTCTCAATTTTATATGATCTCTGATGCAAACAAGCGTATATATGCAATGGGTGACATATATCCAAGTAAATCAAGACTGCCCAAGGGAGATTACAATTTGCGGCTATATCTAAGACATGACAATGTGCAATACTTGGAAAAACTGAAGCAGCTGGTGTTGTTCATCGAGAGAAATTTGGAAGAGAAGGATGTAATCCGGTTGAGCTTCTTCTCCCAACCTGATGGCCCTTTGATGGGAAACGGTAGTTTTAAGTCTTCAGTATTAGTCCCAGGGAAAAAAGAAGCATTCTATTTGGGTCCACCATCCAAAGACAAGCTTCTAAAGTTTTCTTCACAAGGATCTGTATTACTTGGAGCTATCTCGTACGGGAAGCTGTCATATGTTGATAAGGGAGAGGGAAAAAATCCGCTAAAGAACCCTGTGTCTTATCAGATTTCTTATATAGTGCCACCAAATAAGATGGACGAGGACAAAGAAAAAGGTTCTTCTACTTCCACCAAGCCTGTTTCTGAGCGGTTAAAAGAAGAGGTTCGAGATGCAAAGATTAAAGTTCTTGCAAGCCTGAAGCAAGATACTGAGGAAGATTGTTTGGAGTGGAAAAAATTATCGTCTTCTCTTAAGTCTGAATATCCTAAATACACCCCACTGTTTGCAAAGATCTTGGAAGCTGTACTTTCTCGGAGCAATGACAAGGACAAAGTCTGCCATGAAAAAGAGGTAATTGATGCAGCGAATGAGGTGGTTGACAGCGTTGATAGAGACGAGCTGGCCAAATTTTTTGCACTAAGAAGTGATCCCGATGATGAAGAGGCAGAGAAAATTAAAAAGAAGATGGAGACAACTCGTGATCGATTAGCAGAGGCATTGTACCAGAAAGGACTGGCACTGGCAGAGATTGAATCTTTGCAGCCTGCAAAGGCTGAAGGTGCAGAAGGGGGAGAGAAGATTGAAGATCTTTTGAAACCAGGCTCAGATTTGTTTGAAAGCAACTTTAAAGAACTTCAAAAATGGGTTGAAGTGAAGTCCTCCAAATACGGAACTCTCTCAGTACTACGTGAGAAACGTTCTGGAAGGCTTGGAACAGCACTGAAGGTTCTGAATGACGTAATTCAAGACGATGGGGAGCCTCCCAAGAAGAAGTTCTACGACCTGAAGATTTCCTTGCTCGATGAGATTGGCTGGCAACATTTGGCGGCACACGAGAGGCAATGGATGCATGTCCGCTTTCCACCAAGTTTACCTCTTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004175 GO:0004177 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0008240 GO:0009507 GO:0009536 GO:0009987 GO:0016020 GO:0016567 GO:0016604 GO:0016787 GO:0017171 GO:0019538 GO:0022626 GO:0031090 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044451 GO:0044464 GO:0070011 GO:0070013 GO:0070647 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S8 TPPII

Protein Analysis

1386

Amino Acids

153.0

Weight (kDa)

6.48

Isoelectric Point (pI)

41.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	131 - 594	9e-45	Subtilase family
TPPII_Ig-like-1	PF21223	641 - 747	8e-22	Tripeptidyl-peptidase II, first Ig-like domain
TPPII_GBD	PF21316	767 - 851	1.9e-14	TPPII, galactose-binding domain-like
TPPII	PF12580	893 - 1077	3.2e-60	Tripeptidyl peptidase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20850
fragaria_vesca	FvH4_7g29630 FvH4_7g29630 FvH4_7g29630
malus_domestica	MD01G1203100.v1.1 MD07G1272600.v1.1
prunus_persica	Prupe.2G295900_v2.0.a1 Prupe.2G295900_v2.0.a1
pyrus_communis	pycom01g21240 pycom07g24900
rosa_chinensis	RchiOBHm_Chr1g0377641
rosa_laevigata	RLG00000026509
rosa_multiflora	Rmu_sc0002832.1_g000013
rosa_roxburghii	Rroxscaffold_4G00281150
rosa_rugosa	Rorug01G0403900
rosa_samantha	Rh1AG428700 Rh1BG380700 Rh1CG394700 Rh1DG411700
rosa_wichuraiana	Rw1G037000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	375
Acc36I	ACCTGC	1 cut(s)	819
Acc65I	GGTACC	1 cut(s)	1433
AccB1I	GGYRCC	3 cut(s)	353, 398, 1433
AccB7I	CCANNNNNTGG	2 cut(s)	953, 1322
AccBSI	CCGCTC	1 cut(s)	3421
AccI	GTMKAC	1 cut(s)	2266
AccII	CGCG	5 cut(s)	82, 396, 804, 2211, 2886
AclI	AACGTT	1 cut(s)	3970
AclWI	GGATC	6 cut(s)	425, 438, 2135, 2148, 3253, 3695
AcoI	YGGCCR	3 cut(s)	391, 2738, 3675
AcsI	RAATTY	6 cut(s)	1932, 1997, 2727, 2900, 3079, 3680
AcyI	GRCGYC	2 cut(s)	354, 399
AdeI	CACNNNGTG	1 cut(s)	1430
AflII	CTTAAG	2 cut(s)	1939, 3524
AgeI	ACCGGT	1 cut(s)	2701
AjnI	CCWGG	7 cut(s)	1168, 1426, 1513, 1591, 2305, 3175, 3871
AjuI	GAANNNNNNNTTGG	4 cut(s)	2325, 2357, 4031, 4063
Alw21I	GWGCWC	7 cut(s)	802, 1177, 1415, 1426, 1495, 1570, 1591
Alw26I	GTCTC	7 cut(s)	1194, 1217, 1474, 2684, 2913, 3660, 3737
AlwI	GGATC	6 cut(s)	425, 438, 2135, 2148, 3253, 3695
Ama87I	CYCGRG	2 cut(s)	1242, 2287
ApoI	RAATTY	6 cut(s)	1932, 1997, 2727, 2900, 3079, 3680
ArsI	GACNNNNNNTTYG	4 cut(s)	855, 887, 2885, 2917
AseI	ATTAAT	1 cut(s)	2616
AsiGI	ACCGGT	1 cut(s)	2701
Asp700I	GAANNNNTTC	2 cut(s)	3435, 4046
Asp718I	GGTACC	1 cut(s)	1433
AspLEI	GCGC	4 cut(s)	356, 376, 401, 2211
AspS9I	GGNCC	8 cut(s)	808, 866, 1400, 1550, 2213, 2651, 3131, 3203
AsuC2I	CCSGG	2 cut(s)	502, 1862
AsuHPI	GGTGA	5 cut(s)	235, 521, 626, 1529, 2969
AsuII	TTCGAA	2 cut(s)	17, 524
AvaI	CYCGRG	2 cut(s)	1242, 2287
AvaII	GGWCC	2 cut(s)	808, 3203
AxyI	CCTNAGG	1 cut(s)	1850
BaeGI	GKGCMC	1 cut(s)	978
BalI	TGGCCA	1 cut(s)	3677
BamHI	GGATCC	2 cut(s)	430, 2140
BanI	GGYRCC	3 cut(s)	353, 398, 1433
BanII	GRGCYC	4 cut(s)	802, 1249, 1570, 2089
BarI	GAAGNNNNNNTAC	2 cut(s)	4034, 4066
BauI	CACGAG	3 cut(s)	1926, 3750, 4106
BbsI	GAAGAC	5 cut(s)	154, 169, 2847, 3153, 3510
Bbv12I	GWGCWC	7 cut(s)	802, 1177, 1415, 1426, 1495, 1570, 1591
BbvCI	CCTCAGC	1 cut(s)	1716
BceAI	ACGGC	2 cut(s)	406, 1530
BciT130I	CCWGG	7 cut(s)	1170, 1428, 1515, 1593, 2307, 3177, 3873
BclI	TGATCA	2 cut(s)	757, 1281
BcnI	CCSGG	2 cut(s)	502, 1862
BcoDI	GTCTC	7 cut(s)	1194, 1217, 1474, 2684, 2913, 3660, 3737
BfaI	CTAG	5 cut(s)	482, 1542, 1950, 2013, 2550
BfmI	CTRYAG	3 cut(s)	190, 1776, 2250
BfoI	RGCGCY	2 cut(s)	357, 402
BfrI	CTTAAG	2 cut(s)	1939, 3524
BfuAI	ACCTGC	1 cut(s)	819
BglII	AGATCT	2 cut(s)	3564, 3859
BmcAI	AGTACT	1 cut(s)	3957
Bme1390I	CCNGG	9 cut(s)	502, 1170, 1428, 1515, 1593, 1862, 2307, 3177, 3873
Bme18I	GGWCC	2 cut(s)	808, 3203
BmeT110I	CYCGRG	2 cut(s)	1242, 2287
BmgT120I	GGNCC	8 cut(s)	808, 866, 1400, 1550, 2213, 2651, 3131, 3203
BmrFI	CCNGG	9 cut(s)	502, 1170, 1428, 1515, 1593, 1862, 2307, 3177, 3873
BmrI	ACTGGG	1 cut(s)	1242
BmsI	GCATC	9 cut(s)	361, 385, 584, 868, 1018, 2920, 3433, 3622, 4110
BmuI	ACTGGG	1 cut(s)	1242
BpiI	GAAGAC	5 cut(s)	154, 169, 2847, 3153, 3510
BpmI	CTGGAG	3 cut(s)	444, 1191, 1497
Bpu10I	CCTNAGC	1 cut(s)	1716
Bpu14I	TTCGAA	2 cut(s)	17, 524
BpuEI	CTTGAG	4 cut(s)	956, 1547, 1778, 2847
BpuMI	CCSGG	2 cut(s)	502, 1862
Bsa29I	ATCGAT	3 cut(s)	361, 369, 3757
BsaAI	YACGTR	1 cut(s)	3962
BsaBI	GATNNNNATC	2 cut(s)	375, 1092
BsaHI	GRCGYC	2 cut(s)	354, 399
BsaJI	CCNNGG	7 cut(s)	394, 729, 1546, 2204, 2988, 3175, 3176
BsaWI	WCCGGW	3 cut(s)	611, 2701, 3102
BsaXI	ACNNNNNCTCC	2 cut(s)	2838, 2868
Bse118I	RCCGGY	3 cut(s)	2701, 2736, 2740
Bse1I	ACTGG	8 cut(s)	1231, 1237, 1557, 1813, 2064, 2685, 3792, 3798
Bse21I	CCTNAGG	1 cut(s)	1850
Bse3DI	GCAATG	6 cut(s)	1485, 1691, 1713, 2958, 3598, 4121
Bse8I	GATNNNNATC	2 cut(s)	375, 1092
BseBI	CCWGG	7 cut(s)	1170, 1428, 1515, 1593, 2307, 3177, 3873
BseCI	ATCGAT	3 cut(s)	361, 369, 3757
BseDI	CCNNGG	7 cut(s)	394, 729, 1546, 2204, 2988, 3175, 3176
BseGI	GGATG	9 cut(s)	376, 709, 946, 1093, 1870, 2298, 3100, 3209, 4125
BseJI	GATNNNNATC	2 cut(s)	375, 1092
BseMI	GCAATG	6 cut(s)	1485, 1691, 1713, 2958, 3598, 4121
BseMII	CTCAG	8 cut(s)	158, 1707, 2679, 2805, 3408, 3474, 3891, 3966
BseNI	ACTGG	8 cut(s)	1231, 1237, 1557, 1813, 2064, 2685, 3792, 3798
BseSI	GKGCMC	1 cut(s)	978
BseX3I	CGGCCG	2 cut(s)	391, 2738
BseYI	CCCAGC	1 cut(s)	27
BsgI	GTGCAG	3 cut(s)	1617, 2150, 3855
Bsh1236I	CGCG	5 cut(s)	82, 396, 804, 2211, 2886
Bsh1285I	CGRYCG	3 cut(s)	361, 394, 2741
BshNI	GGYRCC	3 cut(s)	353, 398, 1433
BshTI	ACCGGT	1 cut(s)	2701
BshVI	ATCGAT	3 cut(s)	361, 369, 3757
BsiEI	CGRYCG	3 cut(s)	361, 394, 2741
BsiHKAI	GWGCWC	7 cut(s)	802, 1177, 1415, 1426, 1495, 1570, 1591
BsiHKCI	CYCGRG	2 cut(s)	1242, 2287
BsiSI	CCGG	7 cut(s)	501, 612, 1861, 2702, 2737, 2741, 3103
BsiWI	CGTACG	1 cut(s)	3270
BslFI	GGGAC	3 cut(s)	287, 2412, 3158
BsmAI	GTCTC	7 cut(s)	1194, 1217, 1474, 2684, 2913, 3660, 3737
BsmBI	CGTCTC	1 cut(s)	3660
BsmFI	GGGAC	3 cut(s)	287, 2412, 3158
BsmI	GAATGC	1 cut(s)	3191
BsoBI	CYCGRG	2 cut(s)	1242, 2287
Bsp119I	TTCGAA	2 cut(s)	17, 524
Bsp1407I	TGTACA	1 cut(s)	1034
Bsp19I	CCATGG	1 cut(s)	2204
Bsp68I	TCGCGA	2 cut(s)	82, 804
BspCNI	CTCAG	8 cut(s)	159, 1708, 2680, 2804, 3409, 3475, 3890, 3965
BspDI	ATCGAT	3 cut(s)	361, 369, 3757
BspFNI	CGCG	5 cut(s)	82, 396, 804, 2211, 2886
BspHI	TCATGA	2 cut(s)	1644, 2002
BspMI	ACCTGC	1 cut(s)	819
BspPI	GGATC	6 cut(s)	425, 438, 2135, 2148, 3253, 3695
BspT104I	TTCGAA	2 cut(s)	17, 524
BspT107I	GGYRCC	3 cut(s)	353, 398, 1433
BspTI	CTTAAG	2 cut(s)	1939, 3524
BsrBI	CCGCTC	1 cut(s)	3421
BsrDI	GCAATG	6 cut(s)	1485, 1691, 1713, 2958, 3598, 4121
BsrFI	RCCGGY	3 cut(s)	2701, 2736, 2740
BsrGI	TGTACA	1 cut(s)	1034
BsrI	ACTGG	8 cut(s)	1231, 1237, 1557, 1813, 2064, 2685, 3792, 3798
BssAI	RCCGGY	3 cut(s)	2701, 2736, 2740
BssECI	CCNNGG	7 cut(s)	394, 729, 1546, 2204, 2988, 3175, 3176
BssNI	GRCGYC	2 cut(s)	354, 399
BssSI	CACGAG	3 cut(s)	1926, 3750, 4106
BssT1I	CCWWGG	3 cut(s)	729, 2204, 2988
Bst2BI	CACGAG	3 cut(s)	1926, 3750, 4106
Bst2UI	CCWGG	7 cut(s)	1170, 1428, 1515, 1593, 2307, 3177, 3873
Bst4CI	ACNGT	8 cut(s)	278, 1417, 1742, 1981, 2181, 2277, 3149, 3555
Bst6I	CTCTTC	6 cut(s)	598, 1632, 2037, 3081, 3426, 3708
BstACI	GRCGYC	2 cut(s)	354, 399
BstAFI	CTTAAG	2 cut(s)	1939, 3524
BstAPI	GCANNNNNTGC	5 cut(s)	207, 1484, 1604, 2705, 4120
BstAUI	TGTACA	1 cut(s)	1034
BstBAI	YACGTR	1 cut(s)	3962
BstBI	TTCGAA	2 cut(s)	17, 524
BstDSI	CCRYGG	3 cut(s)	394, 1546, 2204
BstF5I	GGATG	9 cut(s)	376, 709, 946, 1093, 1870, 2298, 3100, 3209, 4125
BstFNI	CGCG	5 cut(s)	82, 396, 804, 2211, 2886
BstH2I	RGCGCY	2 cut(s)	357, 402
BstHHI	GCGC	4 cut(s)	356, 376, 401, 2211
BstMAI	GTCTC	7 cut(s)	1194, 1217, 1474, 2684, 2913, 3660, 3737
BstMCI	CGRYCG	3 cut(s)	361, 394, 2741
BstNI	CCWGG	7 cut(s)	1170, 1428, 1515, 1593, 2307, 3177, 3873
BstNSI	RCATGY	3 cut(s)	626, 1642, 4127
BstSCI	CCNGG	9 cut(s)	500, 1168, 1426, 1513, 1591, 1860, 2305, 3175, 3871
BstSFI	CTRYAG	3 cut(s)	190, 1776, 2250
BstSLI	GKGCMC	1 cut(s)	978
BstUI	CGCG	5 cut(s)	82, 396, 804, 2211, 2886
BstV2I	GAAGAC	5 cut(s)	154, 169, 2847, 3153, 3510
BstX2I	RGATCY	5 cut(s)	430, 2140, 3245, 3564, 3859
BstXI	CCANNNNNNTGG	4 cut(s)	322, 1100, 1878, 3128
BstYI	RGATCY	5 cut(s)	430, 2140, 3245, 3564, 3859
BstZI	CGGCCG	2 cut(s)	391, 2738
Bsu15I	ATCGAT	3 cut(s)	361, 369, 3757
Bsu36I	CCTNAGG	1 cut(s)	1850
BsuTUI	ATCGAT	3 cut(s)	361, 369, 3757
BtgI	CCRYGG	3 cut(s)	394, 1546, 2204
BtgZI	GCGATG	1 cut(s)	391
BtsCI	GGATG	9 cut(s)	376, 709, 946, 1093, 1870, 2298, 3100, 3209, 4125
BtsI	GCAGTG	2 cut(s)	217, 1307
BtuMI	TCGCGA	2 cut(s)	82, 804
BveI	ACCTGC	1 cut(s)	819
CciI	TCATGA	2 cut(s)	1644, 2002
CfoI	GCGC	4 cut(s)	356, 376, 401, 2211
Cfr10I	RCCGGY	3 cut(s)	2701, 2736, 2740
Cfr13I	GGNCC	8 cut(s)	808, 866, 1400, 1550, 2213, 2651, 3131, 3203
Cfr42I	CCGCGG	1 cut(s)	397
ClaI	ATCGAT	3 cut(s)	361, 369, 3757
CseI	GACGC	1 cut(s)	1644
CsiI	ACCWGGT	1 cut(s)	1426
CspAI	ACCGGT	1 cut(s)	2701
CspCI	CAANNNNNGTGG	2 cut(s)	2562, 2597
DinI	GGCGCC	2 cut(s)	355, 400
DraI	TTTAAA	1 cut(s)	3901
DraIII	CACNNNGTG	1 cut(s)	1430
EaeI	YGGCCR	3 cut(s)	391, 2738, 3675
EagI	CGGCCG	2 cut(s)	391, 2738
Eam1104I	CTCTTC	6 cut(s)	598, 1632, 2037, 3081, 3426, 3708
EarI	CTCTTC	6 cut(s)	598, 1632, 2037, 3081, 3426, 3708
EciI	GGCGGA	1 cut(s)	276
Ecl136II	GAGCTC	2 cut(s)	800, 1568
EclXI	CGGCCG	2 cut(s)	391, 2738
Eco130I	CCWWGG	3 cut(s)	729, 2204, 2988
Eco147I	AGGCCT	1 cut(s)	2440
Eco24I	GRGCYC	4 cut(s)	802, 1249, 1570, 2089
Eco47I	GGWCC	2 cut(s)	808, 3203
Eco52I	CGGCCG	2 cut(s)	391, 2738
Eco53kI	GAGCTC	2 cut(s)	800, 1568
Eco81I	CCTNAGG	1 cut(s)	1850
Eco88I	CYCGRG	2 cut(s)	1242, 2287
EcoICRI	GAGCTC	2 cut(s)	800, 1568
EcoO109I	RGGNCCY	2 cut(s)	808, 866
EcoRI	GAATTC	1 cut(s)	2727
EcoRII	CCWGG	7 cut(s)	1168, 1426, 1513, 1591, 2305, 3175, 3871
EcoT14I	CCWWGG	3 cut(s)	729, 2204, 2988
EcoT22I	ATGCAT	1 cut(s)	4125
EcoT38I	GRGCYC	4 cut(s)	802, 1249, 1570, 2089
EgeI	GGCGCC	2 cut(s)	355, 400
EheI	GGCGCC	2 cut(s)	355, 400
ErhI	CCWWGG	3 cut(s)	729, 2204, 2988
Esp3I	CGTCTC	1 cut(s)	3660
FaqI	GGGAC	3 cut(s)	287, 2412, 3158
FauNDI	CATATG	2 cut(s)	1465, 3286
FbaI	TGATCA	2 cut(s)	757, 1281
FblI	GTMKAC	1 cut(s)	2266
FokI	GGATG	9 cut(s)	363, 716, 953, 1080, 1877, 2305, 3107, 3196, 4132
FriOI	GRGCYC	4 cut(s)	802, 1249, 1570, 2089
FspAI	RTGCGCAY	1 cut(s)	375
FspBI	CTAG	5 cut(s)	482, 1542, 1950, 2013, 2550
FspI	TGCGCA	1 cut(s)	375
GlaI	GCGC	4 cut(s)	355, 375, 400, 2210
GsaI	CCCAGC	1 cut(s)	31
GsuI	CTGGAG	3 cut(s)	444, 1191, 1497
HaeII	RGCGCY	2 cut(s)	357, 402
HapII	CCGG	7 cut(s)	501, 612, 1861, 2702, 2737, 2741, 3103
HgaI	GACGC	1 cut(s)	1644
HhaI	GCGC	4 cut(s)	356, 376, 401, 2211
Hin1I	GRCGYC	2 cut(s)	354, 399
Hin6I	GCGC	4 cut(s)	354, 374, 399, 2209
HinP1I	GCGC	4 cut(s)	354, 374, 399, 2209
HincII	GTYRAC	8 cut(s)	589, 666, 787, 1239, 1336, 1831, 2267, 3652
HindII	GTYRAC	8 cut(s)	589, 666, 787, 1239, 1336, 1831, 2267, 3652
HindIII	AAGCTT	2 cut(s)	2488, 3221
HpaI	GTTAAC	1 cut(s)	787
HpaII	CCGG	7 cut(s)	501, 612, 1861, 2702, 2737, 2741, 3103
HphI	GGTGA	5 cut(s)	235, 521, 626, 1529, 2969
Hpy99I	CGWCG	3 cut(s)	82, 392, 1638
HpyCH4III	ACNGT	8 cut(s)	278, 1417, 1742, 1981, 2181, 2277, 3149, 3555
HpyCH4IV	ACGT	8 cut(s)	317, 458, 662, 1207, 2800, 3961, 3970, 4011
HpySE526I	ACGT	8 cut(s)	317, 458, 662, 1207, 2800, 3961, 3970, 4011
Hsp92I	GRCGYC	2 cut(s)	354, 399
HspAI	GCGC	4 cut(s)	354, 374, 399, 2209
KasI	GGCGCC	2 cut(s)	353, 398
KpnI	GGTACC	1 cut(s)	1437
Ksp22I	TGATCA	2 cut(s)	757, 1281
KspAI	GTTAAC	1 cut(s)	787
KspI	CCGCGG	1 cut(s)	397
LweI	GCATC	9 cut(s)	361, 385, 584, 868, 1018, 2920, 3433, 3622, 4110
MabI	ACCWGGT	1 cut(s)	1426
MaeI	CTAG	5 cut(s)	482, 1542, 1950, 2013, 2550
MaeII	ACGT	8 cut(s)	317, 458, 662, 1207, 2800, 3961, 3970, 4011
MaeIII	GTNAC	6 cut(s)	454, 1081, 2233, 2461, 2777, 2957
MbiI	CCGCTC	1 cut(s)	3421
MflI	RGATCY	5 cut(s)	430, 2140, 3245, 3564, 3859
MlsI	TGGCCA	1 cut(s)	3677
MluNI	TGGCCA	1 cut(s)	3677
Mly113I	GGCGCC	2 cut(s)	354, 399
MmeI	TCCRAC	2 cut(s)	1399, 1984
Mox20I	TGGCCA	1 cut(s)	3677
Mph1103I	ATGCAT	1 cut(s)	4125
MroXI	GAANNNNTTC	2 cut(s)	3435, 4046
MscI	TGGCCA	1 cut(s)	3677
MslI	CAYNNNNRTG	7 cut(s)	1098, 1272, 1643, 2007, 2064, 2135, 3027
Msp20I	TGGCCA	1 cut(s)	3677
MspA1I	CMGCKG	4 cut(s)	396, 437, 1601, 3058
MspCI	CTTAAG	2 cut(s)	1939, 3524
MspI	CCGG	7 cut(s)	501, 612, 1861, 2702, 2737, 2741, 3103
MspR9I	CCNGG	9 cut(s)	502, 1170, 1428, 1515, 1593, 1862, 2307, 3177, 3873
Mva1269I	GAATGC	1 cut(s)	3191
MvaI	CCWGG	7 cut(s)	1170, 1428, 1515, 1593, 2307, 3177, 3873
MvnI	CGCG	5 cut(s)	82, 396, 804, 2211, 2886
NarI	GGCGCC	2 cut(s)	354, 399
NciI	CCSGG	2 cut(s)	502, 1862
NcoI	CCATGG	1 cut(s)	2204
NdeI	CATATG	2 cut(s)	1465, 3286
NmeAIII	GCCGAG	1 cut(s)	114
NmuCI	GTSAC	2 cut(s)	2777, 2957
NruI	TCGCGA	2 cut(s)	82, 804
NsbI	TGCGCA	1 cut(s)	375
NsiI	ATGCAT	1 cut(s)	4125
NspI	RCATGY	3 cut(s)	626, 1642, 4127
NspV	TTCGAA	2 cut(s)	17, 524
PaeI	GCATGC	1 cut(s)	1642
PaeR7I	CTCGAG	2 cut(s)	1242, 2287
PagI	TCATGA	2 cut(s)	1644, 2002
PasI	CCCWGGG	1 cut(s)	3176
PceI	AGGCCT	1 cut(s)	2440
PcsI	WCGNNNNNNNCGW	2 cut(s)	414, 3967
PctI	GAATGC	1 cut(s)	3191
PdmI	GAANNNNTTC	2 cut(s)	3435, 4046
PfeI	GAWTC	5 cut(s)	19, 363, 419, 2226, 3806
Pfl23II	CGTACG	1 cut(s)	3270
PflFI	GACNNNGTC	1 cut(s)	3605
PflMI	CCANNNNNTGG	2 cut(s)	953, 1322
PinAI	ACCGGT	1 cut(s)	2701
Ple19I	CGATCG	1 cut(s)	361
PluTI	GGCGCC	2 cut(s)	357, 402
Ppu21I	YACGTR	1 cut(s)	3962
PpuMI	RGGWCCY	1 cut(s)	808
PshBI	ATTAAT	1 cut(s)	2616
Psp124BI	GAGCTC	2 cut(s)	802, 1570
Psp1406I	AACGTT	1 cut(s)	3970
Psp5II	RGGWCCY	1 cut(s)	808
Psp6I	CCWGG	7 cut(s)	1168, 1426, 1513, 1591, 2305, 3175, 3871
PspFI	CCCAGC	1 cut(s)	27
PspGI	CCWGG	7 cut(s)	1168, 1426, 1513, 1591, 2305, 3175, 3871
PspLI	CGTACG	1 cut(s)	3270
PspPI	GGNCC	8 cut(s)	808, 866, 1400, 1550, 2213, 2651, 3131, 3203
PspPPI	RGGWCCY	1 cut(s)	808
PspXI	VCTCGAGB	1 cut(s)	1242
PsuI	RGATCY	5 cut(s)	430, 2140, 3245, 3564, 3859
PsyI	GACNNNGTC	1 cut(s)	3605
PvuI	CGATCG	1 cut(s)	361
PvuII	CAGCTG	3 cut(s)	437, 1601, 3058
RruI	TCGCGA	2 cut(s)	82, 804
RseI	CAYNNNNRTG	7 cut(s)	1098, 1272, 1643, 2007, 2064, 2135, 3027
SacI	GAGCTC	2 cut(s)	802, 1570
SacII	CCGCGG	1 cut(s)	397
SalI	GTCGAC	1 cut(s)	2265
Sau96I	GGNCC	8 cut(s)	808, 866, 1400, 1550, 2213, 2651, 3131, 3203
ScaI	AGTACT	1 cut(s)	3957
ScrFI	CCNGG	9 cut(s)	502, 1170, 1428, 1515, 1593, 1862, 2307, 3177, 3873
SexAI	ACCWGGT	1 cut(s)	1426
SfaNI	GCATC	9 cut(s)	361, 385, 584, 868, 1018, 2920, 3433, 3622, 4110
SfcI	CTRYAG	3 cut(s)	190, 1776, 2250
SfoI	GGCGCC	2 cut(s)	355, 400
Sfr274I	CTCGAG	2 cut(s)	1242, 2287
Sfr303I	CCGCGG	1 cut(s)	397
SfuI	TTCGAA	2 cut(s)	17, 524
SgrBI	CCGCGG	1 cut(s)	397
SinI	GGWCC	2 cut(s)	808, 3203
SlaI	CTCGAG	2 cut(s)	1242, 2287
SmiMI	CAYNNNNRTG	7 cut(s)	1098, 1272, 1643, 2007, 2064, 2135, 3027
SmlI	CTYRAG	8 cut(s)	935, 1242, 1526, 1757, 1939, 2287, 2862, 3524
SmoI	CTYRAG	8 cut(s)	935, 1242, 1526, 1757, 1939, 2287, 2862, 3524
SphI	GCATGC	1 cut(s)	1642
SseBI	AGGCCT	1 cut(s)	2440
SspDI	GGCGCC	2 cut(s)	353, 398
SspI	AATATT	1 cut(s)	1069
SspMI	CTAG	5 cut(s)	482, 1542, 1950, 2013, 2550
SstI	GAGCTC	2 cut(s)	802, 1570
StuI	AGGCCT	1 cut(s)	2440
StyD4I	CCNGG	9 cut(s)	500, 1168, 1426, 1513, 1591, 1860, 2305, 3175, 3871
StyI	CCWWGG	3 cut(s)	729, 2204, 2988
TaaI	ACNGT	8 cut(s)	278, 1417, 1742, 1981, 2181, 2277, 3149, 3555
TaiI	ACGT	8 cut(s)	320, 461, 665, 1210, 2803, 3964, 3973, 4014
TaqII	GACCGA	1 cut(s)	166
TatI	WGTACW	4 cut(s)	1034, 1530, 3577, 3955
TauI	GCSGC	6 cut(s)	67, 396, 399, 2214, 3010, 4103
TfiI	GAWTC	5 cut(s)	19, 363, 419, 2226, 3806
TseFI	GTSAC	2 cut(s)	2777, 2957
Tsp45I	GTSAC	2 cut(s)	2777, 2957
TspGWI	ACGGA	3 cut(s)	480, 2859, 3957
Tth111I	GACNNNGTC	1 cut(s)	3605
Van91I	CCANNNNNTGG	2 cut(s)	953, 1322
Vha464I	CTTAAG	2 cut(s)	1939, 3524
VpaK11BI	GGWCC	2 cut(s)	808, 3203
VspI	ATTAAT	1 cut(s)	2616
XapI	RAATTY	6 cut(s)	1932, 1997, 2727, 2900, 3079, 3680
XceI	RCATGY	3 cut(s)	626, 1642, 4127
XhoI	CTCGAG	2 cut(s)	1242, 2287
XmiI	GTMKAC	1 cut(s)	2266
XmnI	GAANNNNTTC	2 cut(s)	3435, 4046
XspI	CTAG	5 cut(s)	482, 1542, 1950, 2013, 2550
ZrmI	AGTACT	1 cut(s)	3957
Zsp2I	ATGCAT	1 cut(s)	4125

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.