pycom01g21540

Belongs to the MT-A70-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005856
Physical Location & Seq
Reverse (-)
19528544 .. 19532159
3616 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2295 bp
ATGATCAAACAACAACTTGGTAGAGAGTGGGAGCTGCCACGGCATGGTCGTGAGAGGACTGACAATGAAAGGCCTCACGGTCGGTCTGGTAATAGGAAAGATGGAAGCCGTGGTGAAGCTGTGAAGACCTCATCAAATTTTGGAATTTCAAATGAAAATTACGATGTGATTGAGATCCAAACTAAGCCTATAGATTACGGAAGAGGAGACTCAGCATCCAACTTTGCTAGGAGGACCGAAGCTGGTCAGCATTCTGATGGGAGATCAGCACCAAGTGATGAAGAGTGGGCATATCTGCAGGATGACAGGACAAGGAGGAGTGATATGTATGGATCTGGACCAGCTAGAGAAGATTCAAAGGAAAGATATTCAGATGATATTAACTCCCTGCGAGATCAGAATTCATGGAGGGAGGACTCTGATGCCTATGGAGGCAAAGGTCAAGGACAGAAAGGTTCTATGCCTGGGCGTGGTGCTGGTGGCCAAAGTTCTGGTGGTGGCTTACAGCCTCCATATGGAAATGCAGAGCCAGGTTCCTTCAATAGAAATGCTTCACAAGGGATGAAAGGGGGTAGAGTAGGTAGAGGAGGAAGGGGTAGGCTTACTGGAAGAGATAGCCAACCGGTAGGAATCCCACTTCCAATAATGGGATCGCCGTTTGGCCCTCTTGGAATGCCACCACCTGGACCCTTGCAGCCACTTACACCTAGCATGTCACCTGCTCCAGGTCCACCAATGTCCCCTGGCGTCTTCATTCCACCATTTTCTCCTCCTGTGTGGCCTGGGGCTCGGGGAGTTGACATGAATATGTTAGGTGTCCCACCTGGTCTCTCCTCGGTCCCTCCTGGATTATCAGGCCCAAGATTTCCTCCTAATATGAGTACTCCGACAAACCCTGCCATGTTTTTTAACCAATCTGGCCATGGAAGAGGAGTTCCTCCAAACATATCTGGCCCTGGTTTTAATTCTGCAGGACCAATGGGACGAGGAACTCCGGCTGATAAAAATCAAGGTGGTTGGGTTCCTCATAAAAGTAGTGGACCTCCTGGTAAAGCTCCTTCCAGAGGAGAGCAGAATGATTACTCGCAAAATTTTGTCGATACTGGTATGCGACCCCAGAATTTCATCAGGGAGCTGGAGCTTACAAATGTTGTGGAGGATTACCCCAAGCTGAGGGAGCTTATACAGAAAAAGGATGAGATTGTGGCTAATTCTGCTTCACATCCCATGTATTACAAGTGCGACCTGAAAGAGTTTGAGCTATCCCCAGAGTTCTTTGGAACCAAGTTTGATGTCATTCTCGTGGATCCACCTTGGGAGGAATACGTTCATCGTGCTCCTGGTGTTGCTGACCACACAGAGTATTGGACGTTTGAAGAAATAATGAATCTTAAGATTGAGGCAATAGCAGACACACCTTCTTTCATTTTTCTTTGGGTGGGTGATGGCATGGGTCTTGAGCAGGGTCGTCAATGTCTAAAAAAGTGGGGATTTCGTAGGTGTGAAGATATATGTTGGGTGAAGACAAACAAAACTAATGCAACTCCTGGTTTGCGCCACGATGCTCATACTTTGTTTCAGCACTCAAAGGAACATTGCTTAATGGGAATAAAAGGCACCGTTCGTCGCAGCACTGATGGCCACATAATTCACGCCAACATTGATACAGATGTGATAATAGCCGAGGAACCCCCGTACGGTTCAACCCAAAAGCCTGAGGATATGTACAGGATTATTGAGCATTTTGCCCTTGGCCGTAGAAGGCTTGAGCTCTTTGGTGAGGACCACAACATTCGGTCCGGCTGGCTGACCGTTGGTAAAGGACTATCCTCATCGAATTTTAATGCCGAGGGATATACCAAGAACTTTGCTGACAAGGACGGGAAGGTTTGGCAAGGAGGGGGTGGACGAAACCCACCTCCAGAGGCACCTCATCTTGTGGTGACAACTCCAGACATAGAGACCCTTCGGCCCAAGTCACCGATGAAAAACCAGCAGCAATTGCAGCAGCAGAATTCATCATCCATTTCTCTTACGCCAGCAAATTCCTCCAACAGAAGACCGGCCGGAAACTCACCGCAAAATCCAATTGCTCTTGGTGTAAACCAAGAAGCTTCAAGTTCTAATCCCTCAACTCCAGCTCCTTGGGGGCCTCCACCAATGGAGGGTTTTAAAGGGCGAGACGGCAACAGTATGCCGTCAGATGATAAGGTTTATGATATGTATGGATACTCAGGACAGCCAAATGGTGAGTTTCTAGATTTTGAGTCTCACAGGCACATGAATTTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MT-A70

Protein Analysis

765

Amino Acids

83.67

Weight (kDa)

6.58

Isoelectric Point (pI)

54.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MT-A70 PF05063 430 - 607 4.4e-52 MT-A70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 727
Acc36I ACCTGC 1 cut(s) 727
AccB1I GGYRCC 2 cut(s) 1616, 1927
AccB7I CCANNNNNTGG 2 cut(s) 44, 683
AciI CCGC 1 cut(s) 2078
AclWI GGATC 5 cut(s) 169, 340, 658, 1301, 1314
AcoI YGGCCR 5 cut(s) 481, 919, 1639, 1753, 2064
AcsI RAATTY 9 cut(s) 136, 144, 400, 1090, 1120, 1837, 2014, 2044, 2284
AcyI GRCGYC 1 cut(s) 747
AdeI CACNNNGTG 1 cut(s) 275
AfaI GTAC 3 cut(s) 883, 1697, 1727
AflII CTTAAG 1 cut(s) 1391
AgeI ACCGGT 1 cut(s) 622
AgsI TTSAA 6 cut(s) 150, 357, 541, 1376, 1704, 2118
AloI GAACNNNNNNTCC 2 cut(s) 918, 950
Alw21I GWGCWC 2 cut(s) 1339, 1773
Alw26I GTCTC 5 cut(s) 201, 833, 1955, 2175, 2274
AlwI GGATC 5 cut(s) 169, 340, 658, 1301, 1314
Ama87I CYCGRG 1 cut(s) 789
ApeKI GCWGC 6 cut(s) 34, 694, 1629, 1996, 2005, 2008
ApoI RAATTY 9 cut(s) 136, 144, 400, 1090, 1120, 1837, 2014, 2044, 2284
AsiGI ACCGGT 1 cut(s) 622
Asp700I GAANNNNTTC 2 cut(s) 550, 1326
AspLEI GCGC 1 cut(s) 1557
AsuHPI GGTGA 9 cut(s) 125, 708, 1454, 1531, 1790, 1954, 1971, 2067, 2261
AvaI CYCGRG 1 cut(s) 789
AvaII GGWCC 9 cut(s) 234, 338, 686, 728, 838, 974, 1040, 1783, 1797
AxyI CCTNAGG 1 cut(s) 1716
BaeI ACNNNNGTAYC 2 cut(s) 1656, 1689
BalI TGGCCA 3 cut(s) 483, 921, 1641
BamHI GGATCC 1 cut(s) 1306
BanI GGYRCC 2 cut(s) 1616, 1927
BanII GRGCYC 2 cut(s) 790, 1773
BarI GAAGNNNNNNTAC 2 cut(s) 1042, 1074
BauI CACGAG 1 cut(s) 1301
BbsI GAAGAC 4 cut(s) 131, 742, 1529, 2065
Bbv12I GWGCWC 2 cut(s) 1339, 1773
BbvCI CCTCAGC 1 cut(s) 1172
BbvI GCAGC 6 cut(s) 21, 706, 1641, 2008, 2017, 2020
BccI CCATC 4 cut(s) 95, 251, 1439, 1631
BceAI ACGGC 6 cut(s) 56, 93, 640, 1740, 2182, 2200
BciVI GTATCC 1 cut(s) 2222
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 5 cut(s) 201, 833, 1955, 2175, 2274
BfaI CTAG 4 cut(s) 228, 345, 708, 2258
BfmI CTRYAG 3 cut(s) 189, 296, 969
BfrI CTTAAG 1 cut(s) 1391
BfuAI ACCTGC 1 cut(s) 727
BfuI GTATCC 1 cut(s) 2222
BisI GCNGC 6 cut(s) 35, 695, 1630, 1997, 2006, 2009
BlsI GCNGC 6 cut(s) 36, 696, 1631, 1998, 2007, 2010
BmcAI AGTACT 1 cut(s) 883
Bme18I GGWCC 9 cut(s) 234, 338, 686, 728, 838, 974, 1040, 1783, 1797
BmeT110I CYCGRG 1 cut(s) 789
BmsI GCATC 3 cut(s) 224, 412, 1552
BpiI GAAGAC 4 cut(s) 131, 742, 1529, 2065
BpmI CTGGAG 5 cut(s) 708, 1157, 1905, 1935, 2121
Bpu10I CCTNAGC 1 cut(s) 1172
BpuEI CTTGAG 2 cut(s) 1478, 1787
BsaBI GATNNNNATC 2 cut(s) 173, 1005
BsaHI GRCGYC 1 cut(s) 747
BsaI GGTCTC 2 cut(s) 833, 1955
BsaWI WCCGGW 1 cut(s) 622
BsaXI ACNNNNNCTCC 2 cut(s) 2125, 2155
Bse118I RCCGGY 2 cut(s) 622, 2062
Bse1I ACTGG 2 cut(s) 610, 1108
Bse21I CCTNAGG 1 cut(s) 1716
Bse3DI GCAATG 1 cut(s) 1594
Bse8I GATNNNNATC 2 cut(s) 173, 1005
BseGI GGATG 6 cut(s) 215, 307, 567, 1201, 1222, 2021
BseJI GATNNNNATC 2 cut(s) 173, 1005
BseMI GCAATG 1 cut(s) 1594
BseMII CTCAG 4 cut(s) 225, 1163, 1707, 2247
BseNI ACTGG 2 cut(s) 610, 1108
BseRI GAGGAG 7 cut(s) 219, 331, 600, 759, 823, 945, 1080
BseX3I CGGCCG 1 cut(s) 2064
BseXI GCAGC 6 cut(s) 21, 706, 1641, 2008, 2017, 2020
Bsh1285I CGRYCG 2 cut(s) 82, 2067
BshNI GGYRCC 2 cut(s) 1616, 1927
BshTI ACCGGT 1 cut(s) 622
BsiEI CGRYCG 2 cut(s) 82, 2067
BsiHKAI GWGCWC 2 cut(s) 1339, 1773
BsiHKCI CYCGRG 1 cut(s) 789
BsiSI CCGG 5 cut(s) 623, 995, 1800, 2063, 2067
BsiWI CGTACG 1 cut(s) 1695
BslFI GGGAC 4 cut(s) 724, 803, 824, 996
BsmAI GTCTC 5 cut(s) 201, 833, 1955, 2175, 2274
BsmBI CGTCTC 1 cut(s) 2175
BsmFI GGGAC 4 cut(s) 724, 803, 824, 996
BsmI GAATGC 2 cut(s) 250, 678
Bso31I GGTCTC 2 cut(s) 833, 1955
BsoBI CYCGRG 1 cut(s) 789
Bsp1286I GDGCHC 3 cut(s) 790, 1339, 1773
Bsp1407I TGTACA 1 cut(s) 1725
Bsp143I GATC 7 cut(s) 3, 174, 263, 332, 394, 650, 1306
Bsp19I CCATGG 1 cut(s) 922
BspACI CCGC 1 cut(s) 2078
BspCNI CTCAG 4 cut(s) 224, 1164, 1708, 2246
BspMAI CTGCAG 2 cut(s) 300, 973
BspMI ACCTGC 1 cut(s) 727
BspPI GGATC 5 cut(s) 169, 340, 658, 1301, 1314
BspT107I GGYRCC 2 cut(s) 1616, 1927
BspTI CTTAAG 1 cut(s) 1391
BspTNI GGTCTC 2 cut(s) 833, 1955
BsrDI GCAATG 1 cut(s) 1594
BsrFI RCCGGY 2 cut(s) 622, 2062
BsrGI TGTACA 1 cut(s) 1725
BsrI ACTGG 2 cut(s) 610, 1108
BssAI RCCGGY 2 cut(s) 622, 2062
BssMI GATC 7 cut(s) 3, 174, 263, 332, 394, 650, 1306
BssNI GRCGYC 1 cut(s) 747
BssSI CACGAG 1 cut(s) 1301
BssT1I CCWWGG 4 cut(s) 922, 1313, 1750, 2144
Bst2BI CACGAG 1 cut(s) 1301
Bst4CI ACNGT 5 cut(s) 80, 1621, 1700, 1813, 2192
Bst6I CTCTTC 4 cut(s) 196, 276, 604, 922
BstACI GRCGYC 1 cut(s) 747
BstAFI CTTAAG 1 cut(s) 1391
BstAPI GCANNNNNTGC 1 cut(s) 2002
BstAUI TGTACA 1 cut(s) 1725
BstC8I GCNNGC 2 cut(s) 1805, 2040
BstDEI CTNAG 5 cut(s) 183, 211, 1172, 1716, 2233
BstDSI CCRYGG 3 cut(s) 38, 109, 922
BstENI CCTNNNNNAGG 2 cut(s) 723, 1062
BstF5I GGATG 6 cut(s) 215, 307, 567, 1201, 1222, 2021
BstHHI GCGC 1 cut(s) 1557
BstKTI GATC 7 cut(s) 6, 177, 266, 335, 397, 653, 1309
BstMAI GTCTC 5 cut(s) 201, 833, 1955, 2175, 2274
BstMBI GATC 7 cut(s) 3, 174, 263, 332, 394, 650, 1306
BstMCI CGRYCG 2 cut(s) 82, 2067
BstMWI GCNNNNNNNGC 6 cut(s) 40, 1177, 1214, 1638, 2002, 2005
BstNSI RCATGY 1 cut(s) 715
BstSFI CTRYAG 3 cut(s) 189, 296, 969
BstV1I GCAGC 6 cut(s) 21, 706, 1641, 2008, 2017, 2020
BstV2I GAAGAC 4 cut(s) 131, 742, 1529, 2065
BstX2I RGATCY 3 cut(s) 174, 332, 1306
BstXI CCANNNNNNTGG 2 cut(s) 491, 2145
BstYI RGATCY 3 cut(s) 174, 332, 1306
BstZI CGGCCG 1 cut(s) 2064
Bsu36I CCTNAGG 1 cut(s) 1716
BsuI GTATCC 1 cut(s) 2222
BtgI CCRYGG 3 cut(s) 38, 109, 922
BtsCI GGATG 6 cut(s) 215, 307, 567, 1201, 1222, 2021
BtsIMutI CAGTG 1 cut(s) 1632
BveI ACCTGC 1 cut(s) 727
Cac8I GCNNGC 2 cut(s) 1805, 2040
CfoI GCGC 1 cut(s) 1557
Cfr10I RCCGGY 2 cut(s) 622, 2062
CpoI CGGWCCG 1 cut(s) 1797
CseI GACGC 1 cut(s) 736
CsiI ACCWGGT 1 cut(s) 823
Csp6I GTAC 3 cut(s) 882, 1696, 1726
CspAI ACCGGT 1 cut(s) 622
CspCI CAANNNNNGTGG 2 cut(s) 1134, 1169
CspI CGGWCCG 1 cut(s) 1797
CviAII CATG 9 cut(s) 44, 405, 712, 802, 901, 923, 1228, 1450, 2281
CviQI GTAC 3 cut(s) 882, 1696, 1726
DdeI CTNAG 5 cut(s) 183, 211, 1172, 1716, 2233
DpnI GATC 7 cut(s) 5, 176, 265, 334, 396, 652, 1308
DpnII GATC 7 cut(s) 3, 174, 263, 332, 394, 650, 1306
DraI TTTAAA 1 cut(s) 2173
DraIII CACNNNGTG 1 cut(s) 275
EaeI YGGCCR 5 cut(s) 481, 919, 1639, 1753, 2064
EagI CGGCCG 1 cut(s) 2064
Eam1104I CTCTTC 4 cut(s) 196, 276, 604, 922
EarI CTCTTC 4 cut(s) 196, 276, 604, 922
Ecl136II GAGCTC 1 cut(s) 1771
EclXI CGGCCG 1 cut(s) 2064
Eco130I CCWWGG 4 cut(s) 922, 1313, 1750, 2144
Eco147I AGGCCT 1 cut(s) 73
Eco24I GRGCYC 2 cut(s) 790, 1773
Eco31I GGTCTC 2 cut(s) 833, 1955
Eco47I GGWCC 9 cut(s) 234, 338, 686, 728, 838, 974, 1040, 1783, 1797
Eco52I CGGCCG 1 cut(s) 2064
Eco53kI GAGCTC 1 cut(s) 1771
Eco81I CCTNAGG 1 cut(s) 1716
Eco88I CYCGRG 1 cut(s) 789
EcoICRI GAGCTC 1 cut(s) 1771
EcoNI CCTNNNNNAGG 2 cut(s) 723, 1062
EcoO109I RGGNCCY 1 cut(s) 2150
EcoRI GAATTC 2 cut(s) 400, 2014
EcoT14I CCWWGG 4 cut(s) 922, 1313, 1750, 2144
EcoT38I GRGCYC 2 cut(s) 790, 1773
ErhI CCWWGG 4 cut(s) 922, 1313, 1750, 2144
Esp3I CGTCTC 1 cut(s) 2175
FaeI CATG 9 cut(s) 47, 408, 715, 805, 904, 926, 1231, 1453, 2284
FaqI GGGAC 4 cut(s) 724, 803, 824, 996
FatI CATG 9 cut(s) 43, 404, 711, 801, 900, 922, 1227, 1449, 2280
FauNDI CATATG 1 cut(s) 514
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 6 cut(s) 35, 695, 1630, 1997, 2006, 2009
FokI GGATG 6 cut(s) 202, 314, 574, 1208, 1209, 2008
FriOI GRGCYC 2 cut(s) 790, 1773
Fsp4HI GCNGC 6 cut(s) 35, 695, 1630, 1997, 2006, 2009
FspBI CTAG 4 cut(s) 228, 345, 708, 2258
GlaI GCGC 1 cut(s) 1556
GluI GCNGC 6 cut(s) 35, 695, 1630, 1997, 2006, 2009
GsuI CTGGAG 5 cut(s) 708, 1157, 1905, 1935, 2121
HapII CCGG 5 cut(s) 623, 995, 1800, 2063, 2067
HgaI GACGC 1 cut(s) 736
HhaI GCGC 1 cut(s) 1557
Hin1I GRCGYC 1 cut(s) 747
Hin1II CATG 9 cut(s) 47, 408, 715, 805, 904, 926, 1231, 1453, 2284
Hin6I GCGC 1 cut(s) 1555
HinP1I GCGC 1 cut(s) 1555
HincII GTYRAC 1 cut(s) 799
HindII GTYRAC 1 cut(s) 799
HindIII AAGCTT 1 cut(s) 2112
HinfI GANTC 6 cut(s) 209, 353, 416, 630, 1387, 2267
HpaII CCGG 5 cut(s) 623, 995, 1800, 2063, 2067
HphI GGTGA 9 cut(s) 125, 708, 1454, 1531, 1790, 1954, 1971, 2067, 2261
Hpy166II GTNNAC 5 cut(s) 731, 799, 1040, 1907, 2104
Hpy188I TCNGA 6 cut(s) 256, 373, 399, 421, 888, 2203
Hpy188III TCNNGA 8 cut(s) 50, 336, 1062, 1457, 1922, 1952, 2235, 2258
Hpy8I GTNNAC 5 cut(s) 731, 799, 1040, 1907, 2104
Hpy99I CGWCG 1 cut(s) 1629
HpyAV CCTTC 7 cut(s) 547, 585, 1068, 1428, 1755, 1879, 1976
HpyCH4III ACNGT 5 cut(s) 80, 1621, 1700, 1813, 2192
HpyCH4IV ACGT 2 cut(s) 1326, 1370
HpyCH4V TGCA 6 cut(s) 298, 524, 694, 971, 1541, 2005
HpyF10VI GCNNNNNNNGC 6 cut(s) 40, 1177, 1214, 1638, 2002, 2005
HpyF3I CTNAG 5 cut(s) 183, 211, 1172, 1716, 2233
HpySE526I ACGT 2 cut(s) 1326, 1370
Hsp92I GRCGYC 1 cut(s) 747
Hsp92II CATG 9 cut(s) 47, 408, 715, 805, 904, 926, 1231, 1453, 2284
HspAI GCGC 1 cut(s) 1555
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 7 cut(s) 3, 174, 263, 332, 394, 650, 1306
LmnI GCTCC 8 cut(s) 31, 727, 1060, 1132, 1138, 1177, 1342, 2146
Lsp1109I GCAGC 6 cut(s) 21, 706, 1641, 2008, 2017, 2020
LweI GCATC 3 cut(s) 224, 412, 1552
MabI ACCWGGT 1 cut(s) 823
MaeI CTAG 4 cut(s) 228, 345, 708, 2258
MaeII ACGT 2 cut(s) 1326, 1370
MaeIII GTNAC 3 cut(s) 714, 1942, 1977
MalI GATC 7 cut(s) 5, 176, 265, 334, 396, 652, 1308
MboI GATC 7 cut(s) 3, 174, 263, 332, 394, 650, 1306
MfeI CAATTG 2 cut(s) 2000, 2088
MflI RGATCY 3 cut(s) 174, 332, 1306
MhlI GDGCHC 3 cut(s) 790, 1339, 1773
MlsI TGGCCA 3 cut(s) 483, 921, 1641
MluNI TGGCCA 3 cut(s) 483, 921, 1641
MlyI GAGTC 3 cut(s) 203, 410, 2276
MmeI TCCRAC 3 cut(s) 243, 911, 2076
Mox20I TGGCCA 3 cut(s) 483, 921, 1641
MroXI GAANNNNTTC 2 cut(s) 550, 1326
MscI TGGCCA 3 cut(s) 483, 921, 1641
MseI TTAA 7 cut(s) 381, 909, 963, 1392, 1601, 1842, 2172
MslI CAYNNNNRTG 4 cut(s) 48, 255, 806, 1301
Msp20I TGGCCA 3 cut(s) 483, 921, 1641
MspCI CTTAAG 1 cut(s) 1391
MspI CCGG 5 cut(s) 623, 995, 1800, 2063, 2067
MunI CAATTG 2 cut(s) 2000, 2088
Mva1269I GAATGC 2 cut(s) 250, 678
MwoI GCNNNNNNNGC 6 cut(s) 40, 1177, 1214, 1638, 2002, 2005
NcoI CCATGG 1 cut(s) 922
NdeI CATATG 1 cut(s) 514
NdeII GATC 7 cut(s) 3, 174, 263, 332, 394, 650, 1306
NlaIII CATG 9 cut(s) 47, 408, 715, 805, 904, 926, 1231, 1453, 2284
NmeAIII GCCGAG 2 cut(s) 1708, 1873
NmuCI GTSAC 3 cut(s) 714, 1942, 1977
NspI RCATGY 1 cut(s) 715
PaqCI CACCTGC 1 cut(s) 727
PceI AGGCCT 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 46
PctI GAATGC 2 cut(s) 250, 678
PdmI GAANNNNTTC 2 cut(s) 550, 1326
PfeI GAWTC 3 cut(s) 353, 630, 1387
Pfl23II CGTACG 1 cut(s) 1695
PflMI CCANNNNNTGG 2 cut(s) 44, 683
PfoI TCCNGGA 1 cut(s) 844
PinAI ACCGGT 1 cut(s) 622
PkrI GCNGC 6 cut(s) 36, 696, 1631, 1998, 2007, 2010
PleI GAGTC 3 cut(s) 203, 410, 2275
PpsI GAGTC 3 cut(s) 203, 410, 2275
Psp124BI GAGCTC 1 cut(s) 1773
PspLI CGTACG 1 cut(s) 1695
PstI CTGCAG 2 cut(s) 300, 973
PsuI RGATCY 3 cut(s) 174, 332, 1306
RsaI GTAC 3 cut(s) 883, 1697, 1727
RsaNI GTAC 3 cut(s) 882, 1696, 1726
RseI CAYNNNNRTG 4 cut(s) 48, 255, 806, 1301
Rsr2I CGGWCCG 1 cut(s) 1797
RsrII CGGWCCG 1 cut(s) 1797
SacI GAGCTC 1 cut(s) 1773
SaqAI TTAA 7 cut(s) 381, 909, 963, 1392, 1601, 1842, 2172
SatI GCNGC 6 cut(s) 35, 695, 1630, 1997, 2006, 2009
Sau3AI GATC 7 cut(s) 3, 174, 263, 332, 394, 650, 1306
ScaI AGTACT 1 cut(s) 883
SchI GAGTC 3 cut(s) 203, 410, 2276
SduI GDGCHC 3 cut(s) 790, 1339, 1773
SexAI ACCWGGT 1 cut(s) 823
SfaNI GCATC 3 cut(s) 224, 412, 1552
SfcI CTRYAG 3 cut(s) 189, 296, 969
SinI GGWCC 9 cut(s) 234, 338, 686, 728, 838, 974, 1040, 1783, 1797
SmiMI CAYNNNNRTG 4 cut(s) 48, 255, 806, 1301
SmlI CTYRAG 3 cut(s) 1391, 1457, 1766
SmoI CTYRAG 3 cut(s) 1391, 1457, 1766
SseBI AGGCCT 1 cut(s) 73
SsiI CCGC 1 cut(s) 2078
SspMI CTAG 4 cut(s) 228, 345, 708, 2258
SstI GAGCTC 1 cut(s) 1773
StuI AGGCCT 1 cut(s) 73
StyI CCWWGG 4 cut(s) 922, 1313, 1750, 2144
TaaI ACNGT 5 cut(s) 80, 1621, 1700, 1813, 2192
TaiI ACGT 2 cut(s) 1329, 1373
TaqI TCGA 2 cut(s) 1098, 1835
TaqII GACCGA 4 cut(s) 72, 251, 826, 1785
TatI WGTACW 2 cut(s) 881, 1725
TfiI GAWTC 3 cut(s) 353, 630, 1387
Tru1I TTAA 7 cut(s) 381, 909, 963, 1392, 1601, 1842, 2172
Tru9I TTAA 7 cut(s) 381, 909, 963, 1392, 1601, 1842, 2172
TscAI CASTG 1 cut(s) 1639
TseFI GTSAC 3 cut(s) 714, 1942, 1977
TseI GCWGC 6 cut(s) 34, 694, 1629, 1996, 2005, 2008
Tsp45I GTSAC 3 cut(s) 714, 1942, 1977
TspGWI ACGGA 1 cut(s) 213
TspRI CASTG 1 cut(s) 1639
Van91I CCANNNNNTGG 2 cut(s) 44, 683
Vha464I CTTAAG 1 cut(s) 1391
VpaK11BI GGWCC 9 cut(s) 234, 338, 686, 728, 838, 974, 1040, 1783, 1797
XagI CCTNNNNNAGG 2 cut(s) 723, 1062
XapI RAATTY 9 cut(s) 136, 144, 400, 1090, 1120, 1837, 2014, 2044, 2284
XbaI TCTAGA 1 cut(s) 2257
XceI RCATGY 1 cut(s) 715
XcmI CCANNNNNNNNNTGG 2 cut(s) 491, 920
XmnI GAANNNNTTC 2 cut(s) 550, 1326
XspI CTAG 4 cut(s) 228, 345, 708, 2258
ZrmI AGTACT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.