Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0009191

Physical Location & Seq

Genomic Coordinates Forward (+)

21323722 .. 21328370

Gene Structure 4649 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3228 bp

ATGGAGTCGCTGACAACGTACTCCTCTTCCTTGTTGTGCACCTGTCCACTGCTGTCCTCCCATTGTTCCCAATCCAACACCACAGCCTTCAAAATTCCCTCGACCTCCACGAAACCTAAAACCACCTTCACCAACATCGTAGTACAGTCCTCGGTGAGCCCGGACCCCTGGTCTCTCAGCGACGGCAACAACCCCGACCGCCCCAAACCCAGGTCCAAGAACCCCAAAAAGCCCCTCTCCGACGACAACGCCCGCCGCATCATCAAGGCCAAGGCCAACTACCTCAGCGCCCTCCGCCGGAACCAGGGCCCACAGGCCCAAACCCCCAAGTGGATTAAGCGGACCCCCGAGCAGATGGTCAGCTACTTGCACGACGACAGGAACGGCCACCTCTACGGCCGCCATGTCGTCGCCGCCATTAAGCACGTCAGAGCGCTGTCCGAGAAGTCCGAGGGGAAGTACGACATGCGCGCCGCCATGGCCTCCTTCGTCGGGAAGCTCAGCTTCAGAGAAATGTGTGTGGTGCTCAAGGAGCAGAAGAGCTGGAGGCAAGTCAGGGACTTGTTCTCTTGGATGAAATTGCAGTTAAGCTACCGCCCAAGTGTTATTGTCTACACATTAGTATTGCGGGCATATGGCCAAGTCGGGAAGATCAAGCTGGCTGAGCAAACCTTCTTGGAGATGCTCGAATCTGGGTGTGAACCGGATGAAGTTGCTTGCGGTACCATGCTCTGTACATACGCCAGATGGGGGCGTCACAAGGCTATGCTGGCGTTTTATTCAGCCGTGCAAGAAAGGGGGATTCTACTTTCTGTTGCTGTTTATAATTTTATGTTGTCCTCCTTGCAGAAGAAATCACTCCACGGAATGGTCATAGAAATGTGGAGGCAGATGGTGGACATTGAAGTGGTACCTAATAAATTTACTTATACGGTAGTAATTGGCTCCCTCGTCAAAGAAGGCCTCCACGAGGAGGCTCTTAAGAATTTCATTGAGTTGAAGAATGCTGGAATCGTTCCTGAGGAGGCAACTTATAGTTTGCTCATCAGTTTAAGCACCAAACATGGCAAATTTGATGAAGCTCTTAGACTATATGAAGATATGAGGTCTCTAGGAATAGTTCCGAGCAACTACACTTGTGCTTCACTTCTGACGTTGTATTACAAGAAGGAGGATTACTCCAAAGCCCTTTCTCTCTTTTCAGAAATGGAAAGGAAGAAAATTGCGGCGGATGAAGTGATCTATGGTTTGCTCATTAGAATATATGGAAAACTTGGTCTTTACGAGGATGCTCAGACAGCTTTTGAAGAGATGGAGCAGCTGGGTTTACTTAGTGACCAGAAAACATATTTGGCAATGACACAAGTCCATCTCAATTCAGGGAATTGCGACAAAGCTTTAGAAGTTATGGAACTAATGAAGTCTAGAAAAAACATTTGGCTCTCAAGATTTGCTTATATTGTCTTACTGCAGTGTTATGTTATGAAGGAGGATCTGAGTTCTGCTGAAGTAACATTTCAGGCTCTATCCAAGACTGGACTCCCTGATGCAGGCTCTTGTAATGATATGCTCAATTTGTATATAAAACTGGACTTATTGGAAAAGGCTAAGGATTTTATTACTCAGATAAGAAGAGACCGAGTAGATTTTGACGAGGAGCTTTGCAGGACAGTTGTGAGAGTATATTGCAAGGAGGGAATGCTTAGAGATGCTGAACAGTTTGTAGAAGAACTGGATACAAGAGGATTATGCCAGGATGGTAGATTCGTCCAAACAATTTCTAGTGTTATGTGTGAACACAAGGAAGGCAAATTTGTGACTTTTGACCAGCCTGACACCGTGGCCCTGGGGCTGGTGCTCGGTCTCTATCTGACAGATGGCGATATCAGTAAGACAGAAAAAGTTCTAGAATCATTGCCTGTGACTTCTGTTGGCTTATCAATTGCTAGTCAGCTCATTAAGAACATTATTAGAGAAGGTGATGCATTTAAAGCAGAAACGCATATCAATCAATTGGCCAAGCTTGGCTGCAGGGTGGATGATGCCACAGTTGCTTCTTTGATTAGTTTATATGGAAAGAAACGCAAGCTGACAAAGGCCCTGGAAATATTTACAGCATTTGCAGATTCTCCTTCAGCAAAAAAATTACTATGTAACTCAATGCTTGATGCATATGCCAAGTGTGGTAAACCACAGGAGGCATACGCACTTTACAAACAACTTACAGAAGAAGGACATGATCTGGATGCTGTTGCCATCAGCATAGTTGTCAATGCTTTGACTAACAGTGGGAAACATAGAGAAGCAGAGAATGTCATCCGGGAAAGCCTTGAGCACCACTTGGAGCTGGATACTGTGGCATACAATACTTTTATTAAGGCTATGCTGGAAGCAGGTAGGTTGCATTTTGCATCCAGTATCTATGAGCGCATGCTTTCTGAGGGGGTTGCTCCATCACTTCGGACATATAGCACCATGATCAGTGTTTACGGTCGAGGTCGAAAGCTGGAAAAGGCTGTAGAGATGCTCACTACGGCTCGCAACTCGGGTTTGTCTTTGGATGAGAAGGCATATATGAATCTGGTTAGCTACTATGGGAAAGCTGGAAAAAGGCATGAAGCATCCATGCTATTCAGCAAAATGCGGGAAGAAGGGATACAACCTGGGATGGTCAGTTATAATATAATGATCAATGTATATGCTGCTGGGGGCCTTTATCAAGAAGCAGAGGAATTATTCAAAGCCATGCAGCAAGATGGTTGCTTACCCGACTCCTTTACCTACCTGTCCCTAATTCGAGCTTACACAGAGAGTCTGAAATACTCAGAAGCAGAGGAAACCATTAACTCTATGCATGAAAACGGTGTCCATCCCTCGTGTGCGCATTTTAACCTTTTACTCTCCGCTTTTGCCAAAATCGGTTTGATAGGTGAAGCAGAAAGAATTTATGGAGAACTACATGGAGCTGGTTTGAACCCTGACGTGGCATGTTATCAGACTATGCTGAGAGGTTACATGGACTATGGACATTTAGAAGAAGGGATCGATCTTTTTGAACAGATCAGTAAGTCTGGAGAGGCAGACAGGTTTATTTTGAGCGCAGCAGTGCATTGTTATAAATCTGTGGGAAAGGAACTTGAAGCTGAAAATGTTTTGCATTCCATGAGTAATTTGGGTATTTCATTTCTAGAGAACCTAGAAGTTGGATCCAAGCTAAAGGCCTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

1076

Amino Acids

120.63

Weight (kDa)

6.61

Isoelectric Point (pI)

32.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	200 - 248	2.4e-08	PPR repeat family
PPR_2	PF13041	271 - 319	2.3e-06	PPR repeat family
PPR_long	PF17177	290 - 426	4.2e-10	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	305 - 350	1.3e-08	PPR repeat family
PPR	PF01535	344 - 373	9.1e-06	PPR repeat
PPR_2	PF13041	348 - 384	3.4e-06	PPR repeat family
PPR_3	PF13812	363 - 424	7.9e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	718 - 761	1.2e-06	PPR repeat family
PPR_long	PF17177	723 - 870	9.8e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	773 - 831	6.2e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	784 - 830	3.3e-10	PPR repeat family
PPR_3	PF13812	845 - 900	9.8e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	855 - 901	8.2e-10	PPR repeat family
TPR_24	PF23276	874 - 985	1.9e-06	Fungal tetratrico peptide repeats
PPR_long	PF17177	876 - 987	1.4e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	888 - 935	1.1e-12	PPR repeat family
PPR	PF01535	891 - 921	3.3e-08	PPR repeat
PPR_3	PF13812	912 - 970	6.5e-09	Pentatricopeptide repeat domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0009191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27270 AT5G27270 AT5G27270 AT5G27270 AT5G27270
fragaria_vesca	FvH4_7g33960 FvH4_7g33960
malus_domestica	MD01G1235800.v1.1
prunus_persica	Prupe.2G325900_v2.0.a1 Prupe.2G325900_v2.0.a1
pyrus_communis	pycom01g24390
rosa_chinensis	RchiOBHm_Chr1g0384191
rosa_laevigata	RLG00000026061
rosa_multiflora	Rmu_sc0005139.1_g000006 Rmu_sc0014578.1_g000006
rosa_roxburghii	Rroxscaffold_4G00276870
rosa_rugosa	Rorug01G0445600
rosa_samantha	Rh1AG471100 Rh1BG428800 Rh1CG441500 Rh1DG461600
rosa_wichuraiana	Rw1G040140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	825, 2679, 3117
Acc16I	TGCGCA	1 cut(s)	2883
Acc36I	ACCTGC	1 cut(s)	2384
Acc65I	GGTACC	2 cut(s)	722, 910
AccB1I	GGYRCC	2 cut(s)	722, 910
AccB7I	CCANNNNNTGG	1 cut(s)	868
AccI	GTMKAC	1 cut(s)	612
AccII	CGCG	1 cut(s)	471
AclWI	GGATC	4 cut(s)	1500, 3050, 3201, 3214
AcoI	YGGCCR	4 cut(s)	385, 397, 637, 2016
AcsI	RAATTY	6 cut(s)	93, 920, 985, 1070, 1811, 2943
AcuI	CTGAAG	3 cut(s)	490, 1527, 2118
AcyI	GRCGYC	1 cut(s)	754
AfaI	GTAC	6 cut(s)	20, 144, 461, 724, 736, 912
AfeI	AGCGCT	1 cut(s)	435
AflII	CTTAAG	1 cut(s)	980
AgsI	TTSAA	8 cut(s)	91, 905, 1000, 1307, 2740, 2974, 3056, 3140
AhdI	GACNNNNNGTC	1 cut(s)	169
AjiI	CACGTC	2 cut(s)	427, 2983
AjnI	CCWGG	7 cut(s)	167, 209, 303, 1752, 1845, 2100, 2662
AjuI	GAANNNNNNNTTGG	4 cut(s)	1334, 1366, 1420, 1452
Alw21I	GWGCWC	4 cut(s)	41, 528, 1860, 2337
Alw26I	GTCTC	4 cut(s)	177, 1113, 1629, 1868
Alw44I	GTGCAC	1 cut(s)	37
AlwI	GGATC	4 cut(s)	1500, 3050, 3201, 3214
Ama87I	CYCGRG	2 cut(s)	347, 2545
Aor51HI	AGCGCT	1 cut(s)	435
ApaI	GGGCCC	1 cut(s)	311
ApaLI	GTGCAC	1 cut(s)	37
ApeKI	GCWGC	5 cut(s)	1318, 2028, 2702, 2749, 3101
ApoI	RAATTY	6 cut(s)	93, 920, 985, 1070, 1811, 2943
Asp700I	GAANNNNTTC	1 cut(s)	1902
Asp718I	GGTACC	2 cut(s)	722, 910
AspLEI	GCGC	7 cut(s)	290, 436, 471, 473, 2430, 2884, 3101
AspS9I	GGNCC	9 cut(s)	163, 213, 307, 308, 316, 342, 1843, 2098, 2710
AsuC2I	CCSGG	2 cut(s)	161, 2321
AsuHPI	GGTGA	4 cut(s)	121, 166, 1991, 2942
AvaI	CYCGRG	2 cut(s)	347, 2545
AvaII	GGWCC	3 cut(s)	163, 213, 342
AxyI	CCTNAGG	1 cut(s)	1020
BaeGI	GKGCMC	2 cut(s)	41, 311
BalI	TGGCCA	2 cut(s)	639, 2018
BamHI	GGATCC	1 cut(s)	3206
BanI	GGYRCC	2 cut(s)	722, 910
BanII	GRGCYC	2 cut(s)	161, 311
BarI	GAAGNNNNNNTAC	4 cut(s)	1160, 1192, 2640, 2672
BauI	CACGAG	2 cut(s)	968, 2875
Bbv12I	GWGCWC	4 cut(s)	41, 528, 1860, 2337
BbvCI	CCTCAGC	1 cut(s)	284
BbvI	GCAGC	5 cut(s)	1330, 2015, 2689, 2761, 3113
BceAI	ACGGC	5 cut(s)	199, 400, 412, 770, 2550
BciT130I	CCWGG	7 cut(s)	169, 211, 305, 1754, 1847, 2102, 2664
BciVI	GTATCC	3 cut(s)	1729, 2344, 2649
BclI	TGATCA	2 cut(s)	2478, 2688
BcnI	CCSGG	2 cut(s)	161, 2321
BcoDI	GTCTC	4 cut(s)	177, 1113, 1629, 1868
BfaI	CTAG	7 cut(s)	1112, 1425, 1782, 1907, 1947, 3188, 3197
BfmI	CTRYAG	3 cut(s)	1469, 2029, 2517
BfoI	RGCGCY	2 cut(s)	291, 437
BfrI	CTTAAG	1 cut(s)	980
BfuAI	ACCTGC	1 cut(s)	2384
BfuI	GTATCC	3 cut(s)	1729, 2344, 2649
BglI	GCCNNNNNGGC	1 cut(s)	479
BlpI	GCTNAGC	2 cut(s)	500, 663
Bme1390I	CCNGG	9 cut(s)	161, 169, 211, 305, 1754, 1847, 2102, 2321, 2664
Bme18I	GGWCC	3 cut(s)	163, 213, 342
BmeRI	GACNNNNNGTC	1 cut(s)	169
BmeT110I	CYCGRG	2 cut(s)	347, 2545
BmgBI	CACGTC	2 cut(s)	427, 2983
BmgT120I	GGNCC	9 cut(s)	163, 213, 307, 308, 316, 342, 1843, 2098, 2710
BmiI	GGNNCC	9 cut(s)	165, 302, 309, 344, 724, 912, 946, 2711, 3208
BmrFI	CCNGG	9 cut(s)	161, 169, 211, 305, 1754, 1847, 2102, 2321, 2664
BoxI	GACNNNNGTC	1 cut(s)	1364
BplI	GAGNNNNNCTC	2 cut(s)	1163, 1195
BpmI	CTGGAG	2 cut(s)	565, 3093
Bpu10I	CCTNAGC	2 cut(s)	284, 1608
Bpu1102I	GCTNAGC	2 cut(s)	500, 663
BpuEI	CTTGAG	3 cut(s)	512, 1429, 2351
BpuMI	CCSGG	2 cut(s)	161, 2321
Bsa29I	ATCGAT	1 cut(s)	3045
BsaHI	GRCGYC	1 cut(s)	754
BsaI	GGTCTC	4 cut(s)	177, 1113, 1629, 1868
BsaWI	WCCGGW	1 cut(s)	703
Bse1I	ACTGG	4 cut(s)	1540, 1593, 1737, 2415
Bse21I	CCTNAGG	1 cut(s)	1020
Bse3DI	GCAATG	2 cut(s)	1362, 1913
BseBI	CCWGG	7 cut(s)	169, 211, 305, 1754, 1847, 2102, 2664
BseCI	ATCGAT	1 cut(s)	3045
BseMI	GCAATG	2 cut(s)	1362, 1913
BseNI	ACTGG	4 cut(s)	1540, 1593, 1737, 2415
BsePI	GCGCGC	1 cut(s)	469
BseRI	GAGGAG	4 cut(s)	13, 986, 1037, 1670
BseSI	GKGCMC	2 cut(s)	41, 311
BseX3I	CGGCCG	1 cut(s)	397
BseXI	GCAGC	5 cut(s)	1330, 2015, 2689, 2761, 3113
BseYI	CCCAGC	2 cut(s)	1321, 2705
Bsh1236I	CGCG	1 cut(s)	471
Bsh1285I	CGRYCG	3 cut(s)	199, 400, 2494
BshNI	GGYRCC	2 cut(s)	722, 910
BshVI	ATCGAT	1 cut(s)	3045
BsiEI	CGRYCG	3 cut(s)	199, 400, 2494
BsiHKAI	GWGCWC	4 cut(s)	41, 528, 1860, 2337
BsiHKCI	CYCGRG	2 cut(s)	347, 2545
BsiSI	CCGG	4 cut(s)	161, 298, 704, 2320
BslFI	GGGAC	2 cut(s)	572, 2773
BsmAI	GTCTC	4 cut(s)	177, 1113, 1629, 1868
BsmFI	GGGAC	2 cut(s)	572, 2773
BsmI	GAATGC	3 cut(s)	1009, 1704, 3157
Bso31I	GGTCTC	4 cut(s)	177, 1113, 1629, 1868
BsoBI	CYCGRG	2 cut(s)	347, 2545
Bsp120I	GGGCCC	1 cut(s)	307
Bsp1286I	GDGCHC	6 cut(s)	41, 161, 311, 528, 1860, 2337
Bsp1407I	TGTACA	1 cut(s)	734
Bsp1720I	GCTNAGC	2 cut(s)	500, 663
Bsp19I	CCATGG	1 cut(s)	477
BspDI	ATCGAT	1 cut(s)	3045
BspFNI	CGCG	1 cut(s)	471
BspLI	GGNNCC	9 cut(s)	165, 302, 309, 344, 724, 912, 946, 2711, 3208
BspMAI	CTGCAG	2 cut(s)	1473, 2033
BspMI	ACCTGC	1 cut(s)	2384
BspPI	GGATC	4 cut(s)	1500, 3050, 3201, 3214
BspQI	GCTCTTC	1 cut(s)	533
BspT107I	GGYRCC	2 cut(s)	722, 910
BspTI	CTTAAG	1 cut(s)	980
BspTNI	GGTCTC	4 cut(s)	177, 1113, 1629, 1868
BsrDI	GCAATG	2 cut(s)	1362, 1913
BsrGI	TGTACA	1 cut(s)	734
BsrI	ACTGG	4 cut(s)	1540, 1593, 1737, 2415
BssHII	GCGCGC	1 cut(s)	469
BssNI	GRCGYC	1 cut(s)	754
BssSI	CACGAG	2 cut(s)	968, 2875
BssT1I	CCWWGG	2 cut(s)	270, 477
Bst2BI	CACGAG	2 cut(s)	968, 2875
Bst2UI	CCWGG	7 cut(s)	169, 211, 305, 1754, 1847, 2102, 2664
Bst6I	CTCTTC	4 cut(s)	31, 533, 1302, 1627
BstACI	GRCGYC	1 cut(s)	754
BstAFI	CTTAAG	1 cut(s)	980
BstAUI	TGTACA	1 cut(s)	734
BstDSI	CCRYGG	3 cut(s)	477, 862, 1839
BstENI	CCTNNNNNAGG	2 cut(s)	968, 1548
BstFNI	CGCG	1 cut(s)	471
BstH2I	RGCGCY	2 cut(s)	291, 437
BstHHI	GCGC	7 cut(s)	290, 436, 471, 473, 2430, 2884, 3101
BstMAI	GTCTC	4 cut(s)	177, 1113, 1629, 1868
BstMCI	CGRYCG	3 cut(s)	199, 400, 2494
BstMWI	GCNNNNNNNGC	8 cut(s)	294, 479, 532, 664, 770, 1298, 1991, 2051
BstNI	CCWGG	7 cut(s)	169, 211, 305, 1754, 1847, 2102, 2664
BstNSI	RCATGY	3 cut(s)	469, 2434, 2991
BstPAI	GACNNNNGTC	1 cut(s)	1364
BstSCI	CCNGG	9 cut(s)	159, 167, 209, 303, 1752, 1845, 2100, 2319, 2662
BstSFI	CTRYAG	3 cut(s)	1469, 2029, 2517
BstSLI	GKGCMC	2 cut(s)	41, 311
BstUI	CGCG	1 cut(s)	471
BstV1I	GCAGC	5 cut(s)	1330, 2015, 2689, 2761, 3113
BstX2I	RGATCY	2 cut(s)	1492, 3206
BstYI	RGATCY	2 cut(s)	1492, 3206
BstZI	CGGCCG	1 cut(s)	397
Bsu15I	ATCGAT	1 cut(s)	3045
Bsu36I	CCTNAGG	1 cut(s)	1020
BsuI	GTATCC	3 cut(s)	1729, 2344, 2649
BsuTUI	ATCGAT	1 cut(s)	3045
BtgI	CCRYGG	3 cut(s)	477, 862, 1839
BtrI	CACGTC	2 cut(s)	427, 2983
BtsI	GCAGTG	3 cut(s)	47, 1478, 3111
BtsIMutI	CAGTG	5 cut(s)	47, 1478, 2293, 2488, 3111
BveI	ACCTGC	1 cut(s)	2384
CfoI	GCGC	7 cut(s)	290, 436, 471, 473, 2430, 2884, 3101
Cfr13I	GGNCC	9 cut(s)	163, 213, 307, 308, 316, 342, 1843, 2098, 2710
ClaI	ATCGAT	1 cut(s)	3045
CseI	GACGC	1 cut(s)	743
Csp6I	GTAC	6 cut(s)	19, 143, 460, 723, 735, 911
CviQI	GTAC	6 cut(s)	19, 143, 460, 723, 735, 911
DraI	TTTAAA	1 cut(s)	1990
DriI	GACNNNNNGTC	1 cut(s)	169
EaeI	YGGCCR	4 cut(s)	385, 397, 637, 2016
EagI	CGGCCG	1 cut(s)	397
Eam1104I	CTCTTC	4 cut(s)	31, 533, 1302, 1627
Eam1105I	GACNNNNNGTC	1 cut(s)	169
EarI	CTCTTC	4 cut(s)	31, 533, 1302, 1627
EciI	GGCGGA	2 cut(s)	284, 1244
EclXI	CGGCCG	1 cut(s)	397
Eco130I	CCWWGG	2 cut(s)	270, 477
Eco147I	AGGCCT	2 cut(s)	963, 3221
Eco24I	GRGCYC	2 cut(s)	161, 311
Eco31I	GGTCTC	4 cut(s)	177, 1113, 1629, 1868
Eco32I	GATATC	1 cut(s)	1885
Eco47I	GGWCC	3 cut(s)	163, 213, 342
Eco47III	AGCGCT	1 cut(s)	435
Eco52I	CGGCCG	1 cut(s)	397
Eco57I	CTGAAG	3 cut(s)	490, 1527, 2118
Eco81I	CCTNAGG	1 cut(s)	1020
Eco88I	CYCGRG	2 cut(s)	347, 2545
EcoNI	CCTNNNNNAGG	2 cut(s)	968, 1548
EcoO109I	RGGNCCY	3 cut(s)	307, 2098, 2710
EcoRII	CCWGG	7 cut(s)	167, 209, 303, 1752, 1845, 2100, 2662
EcoRV	GATATC	1 cut(s)	1885
EcoT14I	CCWWGG	2 cut(s)	270, 477
EcoT22I	ATGCAT	3 cut(s)	1987, 2173, 2856
EcoT38I	GRGCYC	2 cut(s)	161, 311
ErhI	CCWWGG	2 cut(s)	270, 477
FalI	AAGNNNNNCTT	4 cut(s)	488, 520, 1438, 1470
FaqI	GGGAC	2 cut(s)	572, 2773
FauI	CCCGC	3 cut(s)	260, 621, 2637
FauNDI	CATATG	2 cut(s)	634, 2173
FbaI	TGATCA	2 cut(s)	2478, 2688
FblI	GTMKAC	1 cut(s)	612
FriOI	GRGCYC	2 cut(s)	161, 311
FspAI	RTGCGCAY	1 cut(s)	2883
FspBI	CTAG	7 cut(s)	1112, 1425, 1782, 1907, 1947, 3188, 3197
FspI	TGCGCA	1 cut(s)	2883
GlaI	GCGC	7 cut(s)	289, 435, 470, 472, 2429, 2883, 3100
GsaI	CCCAGC	2 cut(s)	1325, 2709
GsuI	CTGGAG	2 cut(s)	565, 3093
HaeII	RGCGCY	2 cut(s)	291, 437
HapII	CCGG	4 cut(s)	161, 298, 704, 2320
HgaI	GACGC	1 cut(s)	743
HhaI	GCGC	7 cut(s)	290, 436, 471, 473, 2430, 2884, 3101
Hin1I	GRCGYC	1 cut(s)	754
Hin6I	GCGC	7 cut(s)	288, 434, 469, 471, 2428, 2882, 3099
HinP1I	GCGC	7 cut(s)	288, 434, 469, 471, 2428, 2882, 3099
HindIII	AAGCTT	2 cut(s)	1395, 2021
HpaII	CCGG	4 cut(s)	161, 298, 704, 2320
HphI	GGTGA	4 cut(s)	121, 166, 1991, 2942
Hpy166II	GTNNAC	9 cut(s)	39, 47, 613, 701, 898, 1328, 1796, 2189, 2488
Hpy8I	GTNNAC	9 cut(s)	39, 47, 613, 701, 898, 1328, 1796, 2189, 2488
Hpy99I	CGWCG	5 cut(s)	185, 245, 377, 413, 494
HpyCH4IV	ACGT	4 cut(s)	17, 426, 1154, 2982
HpyF10VI	GCNNNNNNNGC	8 cut(s)	294, 479, 532, 664, 770, 1298, 1991, 2051
HpySE526I	ACGT	4 cut(s)	17, 426, 1154, 2982
Hsp92I	GRCGYC	1 cut(s)	754
HspAI	GCGC	7 cut(s)	288, 434, 469, 471, 2428, 2882, 3099
KpnI	GGTACC	2 cut(s)	726, 914
Ksp22I	TGATCA	2 cut(s)	2478, 2688
LguI	GCTCTTC	1 cut(s)	533
LmnI	GCTCC	7 cut(s)	532, 950, 1315, 1657, 2344, 2455, 2963
Lsp1109I	GCAGC	5 cut(s)	1330, 2015, 2689, 2761, 3113
MaeI	CTAG	7 cut(s)	1112, 1425, 1782, 1907, 1947, 3188, 3197
MaeII	ACGT	4 cut(s)	17, 426, 1154, 2982
MaeIII	GTNAC	7 cut(s)	755, 1334, 1510, 1816, 1921, 2153, 3011
MfeI	CAATTG	2 cut(s)	1941, 2012
MflI	RGATCY	2 cut(s)	1492, 3206
MhlI	GDGCHC	6 cut(s)	41, 161, 311, 528, 1860, 2337
MlsI	TGGCCA	2 cut(s)	639, 2018
MluNI	TGGCCA	2 cut(s)	639, 2018
MlyI	GAGTC	4 cut(s)	14, 1533, 2765, 2821
MmeI	TCCRAC	3 cut(s)	99, 264, 3184
Mox20I	TGGCCA	2 cut(s)	639, 2018
Mph1103I	ATGCAT	3 cut(s)	1987, 2173, 2856
MroXI	GAANNNNTTC	1 cut(s)	1902
MscI	TGGCCA	2 cut(s)	639, 2018
MslI	CAYNNNNRTG	2 cut(s)	878, 905
Msp20I	TGGCCA	2 cut(s)	639, 2018
MspA1I	CMGCKG	1 cut(s)	1321
MspCI	CTTAAG	1 cut(s)	980
MspI	CCGG	4 cut(s)	161, 298, 704, 2320
MspR9I	CCNGG	9 cut(s)	161, 169, 211, 305, 1754, 1847, 2102, 2321, 2664
MunI	CAATTG	2 cut(s)	1941, 2012
Mva1269I	GAATGC	3 cut(s)	1009, 1704, 3157
MvaI	CCWGG	7 cut(s)	169, 211, 305, 1754, 1847, 2102, 2664
MvnI	CGCG	1 cut(s)	471
MwoI	GCNNNNNNNGC	8 cut(s)	294, 479, 532, 664, 770, 1298, 1991, 2051
NciI	CCSGG	2 cut(s)	161, 2321
NcoI	CCATGG	1 cut(s)	477
NdeI	CATATG	2 cut(s)	634, 2173
NlaIV	GGNNCC	9 cut(s)	165, 302, 309, 344, 724, 912, 946, 2711, 3208
NmuCI	GTSAC	4 cut(s)	755, 1334, 1816, 1921
NsbI	TGCGCA	1 cut(s)	2883
NsiI	ATGCAT	3 cut(s)	1987, 2173, 2856
NspI	RCATGY	3 cut(s)	469, 2434, 2991
PaeI	GCATGC	1 cut(s)	2434
PasI	CCCWGGG	1 cut(s)	1846
PauI	GCGCGC	1 cut(s)	469
PceI	AGGCCT	2 cut(s)	963, 3221
PciSI	GCTCTTC	1 cut(s)	533
PcsI	WCGNNNNNNNCGW	2 cut(s)	14, 107
PctI	GAATGC	3 cut(s)	1009, 1704, 3157
PdmI	GAANNNNTTC	1 cut(s)	1902
PfeI	GAWTC	7 cut(s)	689, 802, 1011, 1764, 1910, 2126, 2578
PflMI	CCANNNNNTGG	1 cut(s)	868
PfoI	TCCNGGA	1 cut(s)	2319
PleI	GAGTC	4 cut(s)	13, 1533, 2765, 2820
PpsI	GAGTC	4 cut(s)	13, 1533, 2765, 2820
PshAI	GACNNNNGTC	1 cut(s)	1364
PsiI	TTATAA	3 cut(s)	825, 2679, 3117
Psp6I	CCWGG	7 cut(s)	167, 209, 303, 1752, 1845, 2100, 2662
PspFI	CCCAGC	2 cut(s)	1321, 2705
PspGI	CCWGG	7 cut(s)	167, 209, 303, 1752, 1845, 2100, 2662
PspN4I	GGNNCC	9 cut(s)	165, 302, 309, 344, 724, 912, 946, 2711, 3208
PspOMI	GGGCCC	1 cut(s)	307
PspPI	GGNCC	9 cut(s)	163, 213, 307, 308, 316, 342, 1843, 2098, 2710
PstI	CTGCAG	2 cut(s)	1473, 2033
PsuI	RGATCY	2 cut(s)	1492, 3206
PteI	GCGCGC	1 cut(s)	469
PvuII	CAGCTG	1 cut(s)	1321
RsaI	GTAC	6 cut(s)	20, 144, 461, 724, 736, 912
RsaNI	GTAC	6 cut(s)	19, 143, 460, 723, 735, 911
RseI	CAYNNNNRTG	2 cut(s)	878, 905
SapI	GCTCTTC	1 cut(s)	533
Sau96I	GGNCC	9 cut(s)	163, 213, 307, 308, 316, 342, 1843, 2098, 2710
SchI	GAGTC	4 cut(s)	14, 1533, 2765, 2821
ScrFI	CCNGG	9 cut(s)	161, 169, 211, 305, 1754, 1847, 2102, 2321, 2664
SduI	GDGCHC	6 cut(s)	41, 161, 311, 528, 1860, 2337
SfcI	CTRYAG	3 cut(s)	1469, 2029, 2517
SinI	GGWCC	3 cut(s)	163, 213, 342
SmiMI	CAYNNNNRTG	2 cut(s)	878, 905
SmlI	CTYRAG	4 cut(s)	527, 980, 1444, 2330
SmoI	CTYRAG	4 cut(s)	527, 980, 1444, 2330
SphI	GCATGC	1 cut(s)	2434
SseBI	AGGCCT	2 cut(s)	963, 3221
SspI	AATATT	1 cut(s)	2109
SspMI	CTAG	7 cut(s)	1112, 1425, 1782, 1907, 1947, 3188, 3197
StuI	AGGCCT	2 cut(s)	963, 3221
StyD4I	CCNGG	9 cut(s)	159, 167, 209, 303, 1752, 1845, 2100, 2319, 2662
StyI	CCWWGG	2 cut(s)	270, 477
TaiI	ACGT	4 cut(s)	20, 429, 1157, 2985
TaqI	TCGA	6 cut(s)	101, 687, 2494, 2500, 2797, 3045
TaqII	GACCGA	2 cut(s)	1653, 1850
TatI	WGTACW	2 cut(s)	142, 734
TauI	GCSGC	5 cut(s)	258, 402, 416, 476, 1229
TfiI	GAWTC	7 cut(s)	689, 802, 1011, 1764, 1910, 2126, 2578
TscAI	CASTG	5 cut(s)	54, 1478, 2293, 2488, 3111
TseFI	GTSAC	4 cut(s)	755, 1334, 1816, 1921
TseI	GCWGC	5 cut(s)	1318, 2028, 2702, 2749, 3101
Tsp45I	GTSAC	4 cut(s)	755, 1334, 1816, 1921
TspGWI	ACGGA	1 cut(s)	879
TspRI	CASTG	5 cut(s)	54, 1478, 2293, 2488, 3111
Van91I	CCANNNNNTGG	1 cut(s)	868
Vha464I	CTTAAG	1 cut(s)	980
VneI	GTGCAC	1 cut(s)	37
VpaK11BI	GGWCC	3 cut(s)	163, 213, 342
XagI	CCTNNNNNAGG	2 cut(s)	968, 1548
XapI	RAATTY	6 cut(s)	93, 920, 985, 1070, 1811, 2943
XbaI	TCTAGA	3 cut(s)	1424, 1906, 3187
XceI	RCATGY	3 cut(s)	469, 2434, 2991
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3169
XmiI	GTMKAC	1 cut(s)	612
XmnI	GAANNNNTTC	1 cut(s)	1902
XspI	CTAG	7 cut(s)	1112, 1425, 1782, 1907, 1947, 3188, 3197
Zsp2I	ATGCAT	3 cut(s)	1987, 2173, 2856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.