pycom02g00970

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0004372
Physical Location & Seq
Forward (+)
567294 .. 570565
3272 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1839 bp
ATGGAAGGTTCGGGGAGTACGAGGGTGGAGAAGAGAGGGTACGAATTGGATGCCGATGCAGATGGTCATCCCCCCGAGTCGAAGAAGCAAAGATTGCCCACTTTGGCAAGTGTAATTGTGGAATCTTTGAAGGTAGATAGCTTGCAAAGACTTTGCTCATCTTTGGAGCCTCTTCTCCGCCGAATTGTTAGTGAAGAAGTGGAGCGTGCTTTGACAAAGTTAGACCATGCTGAACTGGCTGGAAGGTCTCCACCACCGAGAATACAGGGTCCAGAAGGAAAAACCCTGCAACTACAATTTAAAACAAGAATGCCACCTCACCTATTCACAGGTGCAAAGGTTGAAGGAGAGCAAGGAGCAGCGATTCCTGTTGTCTTGGTCGACCTCAGCACAGGCAGTGTTATGCAAACAGGACCCGAATCTGCTGCAAAACTGAATGTTGTGGTGCTGGAAGGCGACTTCAATGAGGAATCTGAGGATAATTGGACGAAAGAACACTTTGAAAACCATGAAGTAAAGGAACGTGAGGGGAAAAGGCCTCTTCTGACAGGGGATCTACAGGTTGTTCTCAAGGAAGGGGTAGGTACTCTTGGAGATCTTACCTTCACCGACAATTCTAGCTGGACGAGGAGCAGGAAATTCAGGCTCGGTGTTAAGATTTCCCCTGGATATTGTGAAGGAATTCGTATTTGTGAGGCCAAAACGGATGCCTTTTCTGTTAAAGATCACAGGGGAGAATTGTACAAGAAACACTACCCACCGGCTTTGCACGATGAAGTTTGGAGATTGGATAGGATAGCAAAGGATGGAGCTCTGCACAAAAAACTGATGAAGGCTGAGATCATAACCGTTGAAGACTTCCTTCGCCTTCTTGTCAAGGACCCGCAGAAACTAAGAAGTAGATGCTTGGGAGCGGGATGTCCAACAGGATGTGGGAAAACACAGTGGAGCATGCCAAAACTTGTGTCTTGGGTGGAAAGCTCTATGTTTACTAGATTCGATCAGGCTCATGGCACGGGTGTTGTTTTCAATCACATTTACGAGCTCAGGGGACTTATCGCTGATGGGCAATTTCTTCCCTTGGAGTCACTCACCCACACTCAAAAGGTTTCAGTGGATTCTCTGGTTAAGAGAGCGTACGATAATTGGCAACAAGTCATAGAATATGACGGCAAAGTCTTACATGCTCTCGCCGGAAATAAAAAGGTCACGAGAGCCTCACCTGCACCCGTGGCTGACTACAACAACTATGCTATAATGGATCACCATTCTGCATCTACTCAGAATAGCCAGCAGCAGTACCCCCCTCAATTCAGCCAACAGTTCCAACCCGAAAATAACCACCCATCCGTCTCTCAGTTCATTGAATTTCCATTTGTGAGGTCCAACCAAACAGCGTTGATCAAATTTAATAACCCAGAACCAGCAGCATTGCCTAGCAGCACGGGTTACATGCCATCTGTTGGCGGTTCAGATTTTTCAGGAGATTGGACACGGCCAAACTACGGGAATGGATTGGACGATTTCGTTGCTGATGAAATCCGGATCAGGAGTTCAGAGATGTTGGAGAGTGATGACATGCAAAGACTGCTCAAGACGTTTAGTATGGGAGCCGGCGTAGGAGCAGGAGGAACTGGTTTTGGTGCTTCTGATGAGTCTTGTTACAATTACAGTGTACAATACGAGCCTCAGATAGGCCAGCCGTTCAGGAAGGAGCACGCGAAGAGCTCAGGGAAGGCTGTTGTCGGGTGGCTTAAGCTCAAAGCGGCTTTAAGGTGGGGGATTTTTATTAGGAAGAGAGCTGCAGAAAGGAGAGCTCAGCTTACAGAGCTAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

613

Amino Acids

68.13

Weight (kDa)

6.81

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 96 - 243 2.5e-58 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 255 - 306 4.2e-18 Calmodulin binding protein central domain
Calmod_bind_C PF20452 335 - 389 3.3e-18 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1231
AasI GACNNNNNNGTC 1 cut(s) 1175
Acc36I ACCTGC 1 cut(s) 1231
AccB7I CCANNNNNTGG 1 cut(s) 1463
AccBSI CCGCTC 1 cut(s) 914
AccI GTMKAC 1 cut(s) 381
AccII CGCG 1 cut(s) 1721
AccIII TCCGGA 1 cut(s) 1542
AciI CCGC 5 cut(s) 178, 884, 914, 1467, 1766
AclWI GGATC 3 cut(s) 561, 1269, 1553
AcoI YGGCCR 1 cut(s) 1496
AcsI RAATTY 4 cut(s) 638, 681, 1367, 1406
AfaI GTAC 7 cut(s) 19, 41, 586, 743, 1139, 1301, 1677
AfiI CCNNNNNNNGG 3 cut(s) 1463, 1505, 1694
AflII CTTAAG 1 cut(s) 1754
AgsI TTSAA 7 cut(s) 130, 344, 463, 503, 854, 1030, 1367
AjnI CCWGG 1 cut(s) 664
AloI GAACNNNNNNTCC 2 cut(s) 1537, 1569
Alw21I GWGCWC 5 cut(s) 814, 1047, 1719, 1730, 1819
Alw26I GTCTC 2 cut(s) 252, 1357
AlwI GGATC 3 cut(s) 561, 1269, 1553
Ama87I CYCGRG 1 cut(s) 74
Aor13HI TCCGGA 1 cut(s) 1542
AoxI GGCC 4 cut(s) 536, 696, 1496, 1696
ApeKI GCWGC 6 cut(s) 359, 425, 1294, 1427, 1440, 1802
ApoI RAATTY 4 cut(s) 638, 681, 1367, 1406
ArsI GACNNNNNNTTYG 2 cut(s) 950, 982
Asp700I GAANNNNTTC 1 cut(s) 681
AspS9I GGNCC 4 cut(s) 269, 413, 880, 1383
AsuHPI GGTGA 5 cut(s) 311, 598, 1084, 1212, 1256
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 4 cut(s) 269, 413, 880, 1383
BanII GRGCYC 4 cut(s) 814, 1047, 1730, 1819
BarI GAAGNNNNNNTAC 2 cut(s) 23, 55
BauI CACGAG 1 cut(s) 1210
BbsI GAAGAC 1 cut(s) 861
Bbv12I GWGCWC 5 cut(s) 814, 1047, 1719, 1730, 1819
BbvCI CCTCAGC 1 cut(s) 386
BbvI GCAGC 6 cut(s) 371, 412, 1306, 1439, 1452, 1789
BccI CCATC 5 cut(s) 56, 800, 1058, 1354, 1465
BceAI ACGGC 3 cut(s) 1186, 1511, 1687
BcgI CGANNNNNNTGC 6 cut(s) 32, 66, 407, 441, 1511, 1545
BciT130I CCWGG 1 cut(s) 666
BclI TGATCA 1 cut(s) 1401
BcoDI GTCTC 2 cut(s) 252, 1357
BfaI CTAG 4 cut(s) 618, 993, 1437, 1832
BfmI CTRYAG 2 cut(s) 557, 1803
BfrI CTTAAG 1 cut(s) 1754
BfuAI ACCTGC 1 cut(s) 1231
BglII AGATCT 1 cut(s) 595
BisI GCNGC 7 cut(s) 360, 426, 1295, 1428, 1441, 1767, 1803
BlpI GCTNAGC 1 cut(s) 1818
BlsI GCNGC 7 cut(s) 361, 427, 1296, 1429, 1442, 1768, 1804
Bme1390I CCNGG 1 cut(s) 666
Bme18I GGWCC 4 cut(s) 269, 413, 880, 1383
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 4 cut(s) 269, 413, 880, 1383
BmiI GGNNCC 5 cut(s) 168, 270, 415, 882, 1612
BmrFI CCNGG 1 cut(s) 666
BmsI GCATC 5 cut(s) 40, 46, 697, 893, 1283
BpiI GAAGAC 1 cut(s) 861
Bpu10I CCTNAGC 3 cut(s) 386, 1046, 1729
Bpu1102I GCTNAGC 1 cut(s) 1818
BpuEI CTTGAG 2 cut(s) 554, 1577
BsaBI GATNNNNATC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 252
BsaJI CCNNGG 3 cut(s) 664, 1080, 1230
BsaWI WCCGGW 1 cut(s) 1542
BsaXI ACNNNNNCTCC 2 cut(s) 1602, 1632
Bsc4I CCNNNNNNNGG 3 cut(s) 1463, 1505, 1694
Bse118I RCCGGY 2 cut(s) 760, 1613
Bse1I ACTGG 2 cut(s) 240, 1639
Bse3DI GCAATG 1 cut(s) 1430
Bse8I GATNNNNATC 1 cut(s) 66
BseAI TCCGGA 1 cut(s) 1542
BseBI CCWGG 1 cut(s) 666
BseDI CCNNGG 3 cut(s) 664, 1080, 1230
BseGI GGATG 7 cut(s) 55, 67, 712, 811, 923, 935, 1346
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 3 cut(s) 1463, 1505, 1694
BseMI GCAATG 1 cut(s) 1430
BseMII CTCAG 9 cut(s) 400, 465, 828, 1060, 1295, 1370, 1703, 1743, 1832
BseNI ACTGG 2 cut(s) 240, 1639
BseRI GAGGAG 1 cut(s) 643
BseXI GCAGC 6 cut(s) 371, 412, 1306, 1439, 1452, 1789
BsgI GTGCAG 2 cut(s) 800, 1209
Bsh1236I CGCG 1 cut(s) 1721
BshFI GGCC 4 cut(s) 538, 698, 1498, 1698
BsiHKAI GWGCWC 5 cut(s) 814, 1047, 1719, 1730, 1819
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 4 cut(s) 761, 1194, 1543, 1614
BsiWI CGTACG 1 cut(s) 1137
BslFI GGGAC 1 cut(s) 1065
BslI CCNNNNNNNGG 3 cut(s) 1463, 1505, 1694
BsmAI GTCTC 2 cut(s) 252, 1357
BsmBI CGTCTC 1 cut(s) 1357
BsmFI GGGAC 1 cut(s) 1065
BsmI GAATGC 1 cut(s) 315
BsnI GGCC 4 cut(s) 538, 698, 1498, 1698
Bso31I GGTCTC 1 cut(s) 252
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 5 cut(s) 814, 1047, 1719, 1730, 1819
Bsp13I TCCGGA 1 cut(s) 1542
Bsp1407I TGTACA 2 cut(s) 741, 1675
Bsp143I GATC 8 cut(s) 553, 595, 724, 840, 1000, 1261, 1401, 1545
Bsp1720I GCTNAGC 1 cut(s) 1818
BspACI CCGC 5 cut(s) 178, 884, 914, 1467, 1766
BspANI GGCC 4 cut(s) 538, 698, 1498, 1698
BspCNI CTCAG 9 cut(s) 399, 466, 829, 1059, 1294, 1369, 1702, 1742, 1831
BspEI TCCGGA 1 cut(s) 1542
BspFNI CGCG 1 cut(s) 1721
BspLI GGNNCC 5 cut(s) 168, 270, 415, 882, 1612
BspMAI CTGCAG 1 cut(s) 1807
BspMI ACCTGC 1 cut(s) 1231
BspPI GGATC 3 cut(s) 561, 1269, 1553
BspQI GCTCTTC 1 cut(s) 1718
BspTI CTTAAG 1 cut(s) 1754
BspTNI GGTCTC 1 cut(s) 252
BsrBI CCGCTC 1 cut(s) 914
BsrDI GCAATG 1 cut(s) 1430
BsrFI RCCGGY 2 cut(s) 760, 1613
BsrGI TGTACA 2 cut(s) 741, 1675
BsrI ACTGG 2 cut(s) 240, 1639
BssAI RCCGGY 2 cut(s) 760, 1613
BssECI CCNNGG 3 cut(s) 664, 1080, 1230
BssMI GATC 8 cut(s) 553, 595, 724, 840, 1000, 1261, 1401, 1545
BssSI CACGAG 1 cut(s) 1210
BssT1I CCWWGG 1 cut(s) 1080
Bst2BI CACGAG 1 cut(s) 1210
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 4 cut(s) 850, 945, 1323, 1673
Bst6I CTCTTC 5 cut(s) 26, 177, 546, 1718, 1790
BstAFI CTTAAG 1 cut(s) 1754
BstAPI GCANNNNNTGC 2 cut(s) 94, 1588
BstAUI TGTACA 2 cut(s) 741, 1675
BstC8I GCNNGC 7 cut(s) 143, 207, 953, 1292, 1615, 1700, 1719
BstDSI CCRYGG 1 cut(s) 1230
BstENI CCTNNNNNAGG 1 cut(s) 1692
BstF5I GGATG 7 cut(s) 55, 67, 712, 811, 923, 935, 1346
BstFNI CGCG 1 cut(s) 1721
BstKTI GATC 8 cut(s) 556, 598, 727, 843, 1003, 1264, 1404, 1548
BstMAI GTCTC 2 cut(s) 252, 1357
BstMBI GATC 8 cut(s) 553, 595, 724, 840, 1000, 1261, 1401, 1545
BstMWI GCNNNNNNNGC 6 cut(s) 94, 236, 1223, 1232, 1588, 1828
BstNI CCWGG 1 cut(s) 666
BstNSI RCATGY 4 cut(s) 955, 1187, 1456, 1582
BstSCI CCNGG 1 cut(s) 664
BstSFI CTRYAG 2 cut(s) 557, 1803
BstUI CGCG 1 cut(s) 1721
BstV1I GCAGC 6 cut(s) 371, 412, 1306, 1439, 1452, 1789
BstV2I GAAGAC 1 cut(s) 861
BstX2I RGATCY 2 cut(s) 553, 595
BstYI RGATCY 2 cut(s) 553, 595
BsuRI GGCC 4 cut(s) 538, 698, 1498, 1698
BtgI CCRYGG 1 cut(s) 1230
BtsCI GGATG 7 cut(s) 55, 67, 712, 811, 923, 935, 1346
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 4 cut(s) 403, 950, 1119, 1678
BveI ACCTGC 1 cut(s) 1231
Cac8I GCNNGC 7 cut(s) 143, 207, 953, 1292, 1615, 1700, 1719
Cfr10I RCCGGY 2 cut(s) 760, 1613
Cfr13I GGNCC 4 cut(s) 269, 413, 880, 1383
Csp6I GTAC 7 cut(s) 18, 40, 585, 742, 1138, 1300, 1676
CspCI CAANNNNNGTGG 2 cut(s) 747, 782
CviAII CATG 7 cut(s) 227, 509, 952, 1010, 1184, 1453, 1579
CviQI GTAC 7 cut(s) 18, 40, 585, 742, 1138, 1300, 1676
DpnI GATC 8 cut(s) 555, 597, 726, 842, 1002, 1263, 1403, 1547
DpnII GATC 8 cut(s) 553, 595, 724, 840, 1000, 1261, 1401, 1545
DraI TTTAAA 1 cut(s) 301
DrdI GACNNNNNNGTC 1 cut(s) 1175
DseDI GACNNNNNNGTC 1 cut(s) 1175
EaeI YGGCCR 1 cut(s) 1496
Eam1104I CTCTTC 5 cut(s) 26, 177, 546, 1718, 1790
EarI CTCTTC 5 cut(s) 26, 177, 546, 1718, 1790
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 4 cut(s) 812, 1045, 1728, 1817
Eco130I CCWWGG 1 cut(s) 1080
Eco147I AGGCCT 1 cut(s) 538
Eco24I GRGCYC 4 cut(s) 814, 1047, 1730, 1819
Eco31I GGTCTC 1 cut(s) 252
Eco47I GGWCC 4 cut(s) 269, 413, 880, 1383
Eco53kI GAGCTC 4 cut(s) 812, 1045, 1728, 1817
Eco88I CYCGRG 1 cut(s) 74
EcoICRI GAGCTC 4 cut(s) 812, 1045, 1728, 1817
EcoNI CCTNNNNNAGG 1 cut(s) 1692
EcoO109I RGGNCCY 2 cut(s) 413, 880
EcoRI GAATTC 1 cut(s) 681
EcoRII CCWGG 1 cut(s) 664
EcoT14I CCWWGG 1 cut(s) 1080
EcoT38I GRGCYC 4 cut(s) 814, 1047, 1730, 1819
ErhI CCWWGG 1 cut(s) 1080
Esp3I CGTCTC 1 cut(s) 1357
FaeI CATG 7 cut(s) 230, 512, 955, 1013, 1187, 1456, 1582
FalI AAGNNNNNCTT 2 cut(s) 846, 878
FaqI GGGAC 1 cut(s) 1065
FatI CATG 7 cut(s) 226, 508, 951, 1009, 1183, 1452, 1578
FauI CCCGC 2 cut(s) 891, 907
FbaI TGATCA 1 cut(s) 1401
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 7 cut(s) 360, 426, 1295, 1428, 1441, 1767, 1803
FokI GGATG 7 cut(s) 54, 62, 719, 818, 930, 942, 1333
FriOI GRGCYC 4 cut(s) 814, 1047, 1730, 1819
Fsp4HI GCNGC 7 cut(s) 360, 426, 1295, 1428, 1441, 1767, 1803
FspBI CTAG 4 cut(s) 618, 993, 1437, 1832
GluI GCNGC 7 cut(s) 360, 426, 1295, 1428, 1441, 1767, 1803
HaeIII GGCC 4 cut(s) 538, 698, 1498, 1698
HapII CCGG 4 cut(s) 761, 1194, 1543, 1614
Hin1II CATG 7 cut(s) 230, 512, 955, 1013, 1187, 1456, 1582
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 9 cut(s) 77, 122, 364, 419, 470, 996, 1085, 1118, 1655
HpaII CCGG 4 cut(s) 761, 1194, 1543, 1614
HphI GGTGA 5 cut(s) 311, 598, 1084, 1212, 1256
Hpy166II GTNNAC 3 cut(s) 382, 990, 1676
Hpy188I TCNGA 7 cut(s) 475, 546, 1284, 1474, 1558, 1651, 1692
Hpy188III TCNNGA 7 cut(s) 272, 1210, 1482, 1543, 1549, 1594, 1708
Hpy8I GTNNAC 3 cut(s) 382, 990, 1676
HpyCH4III ACNGT 4 cut(s) 850, 945, 1323, 1673
HpyCH4IV ACGT 2 cut(s) 523, 1598
HpyF10VI GCNNNNNNNGC 6 cut(s) 94, 236, 1223, 1232, 1588, 1828
HpySE526I ACGT 2 cut(s) 523, 1598
Hsp92II CATG 7 cut(s) 230, 512, 955, 1013, 1187, 1456, 1582
Kpn2I TCCGGA 1 cut(s) 1542
KroI GCCGGC 1 cut(s) 1613
KroNI GCCGGC 1 cut(s) 1615
Ksp22I TGATCA 1 cut(s) 1401
Kzo9I GATC 8 cut(s) 553, 595, 724, 840, 1000, 1261, 1401, 1545
LguI GCTCTTC 1 cut(s) 1718
Lsp1109I GCAGC 6 cut(s) 371, 412, 1306, 1439, 1452, 1789
LweI GCATC 5 cut(s) 40, 46, 697, 893, 1283
MaeI CTAG 4 cut(s) 618, 993, 1437, 1832
MaeII ACGT 2 cut(s) 523, 1598
MaeIII GTNAC 4 cut(s) 1086, 1207, 1448, 1661
MalI GATC 8 cut(s) 555, 597, 726, 842, 1002, 1263, 1403, 1547
MbiI CCGCTC 1 cut(s) 914
MboI GATC 8 cut(s) 553, 595, 724, 840, 1000, 1261, 1401, 1545
MboII GAAGA 9 cut(s) 43, 94, 164, 206, 533, 866, 1067, 1735, 1807
MflI RGATCY 2 cut(s) 553, 595
MhlI GDGCHC 5 cut(s) 814, 1047, 1719, 1730, 1819
MlyI GAGTC 3 cut(s) 86, 1094, 1664
MmeI TCCRAC 4 cut(s) 947, 1351, 1410, 1545
MroI TCCGGA 1 cut(s) 1542
MroNI GCCGGC 1 cut(s) 1613
MroXI GAANNNNTTC 1 cut(s) 681
MseI TTAA 7 cut(s) 300, 654, 720, 1128, 1410, 1755, 1772
MspCI CTTAAG 1 cut(s) 1754
MspI CCGG 4 cut(s) 761, 1194, 1543, 1614
MspR9I CCNGG 1 cut(s) 666
Mva1269I GAATGC 1 cut(s) 315
MvaI CCWGG 1 cut(s) 666
MvnI CGCG 1 cut(s) 1721
MwoI GCNNNNNNNGC 6 cut(s) 94, 236, 1223, 1232, 1588, 1828
NaeI GCCGGC 1 cut(s) 1615
NdeII GATC 8 cut(s) 553, 595, 724, 840, 1000, 1261, 1401, 1545
NgoMIV GCCGGC 1 cut(s) 1613
NlaIII CATG 7 cut(s) 230, 512, 955, 1013, 1187, 1456, 1582
NlaIV GGNNCC 5 cut(s) 168, 270, 415, 882, 1612
NmuCI GTSAC 2 cut(s) 1086, 1207
NspI RCATGY 4 cut(s) 955, 1187, 1456, 1582
PaeI GCATGC 1 cut(s) 955
PaqCI CACCTGC 1 cut(s) 1231
PceI AGGCCT 1 cut(s) 538
PciSI GCTCTTC 1 cut(s) 1718
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PctI GAATGC 1 cut(s) 315
PdiI GCCGGC 1 cut(s) 1615
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 6 cut(s) 122, 364, 419, 470, 996, 1118
Pfl23II CGTACG 1 cut(s) 1137
PflMI CCANNNNNTGG 1 cut(s) 1463
PkrI GCNGC 7 cut(s) 361, 427, 1296, 1429, 1442, 1768, 1804
PleI GAGTC 3 cut(s) 85, 1093, 1663
PpsI GAGTC 3 cut(s) 85, 1093, 1663
PpuMI RGGWCCY 2 cut(s) 413, 880
Psp124BI GAGCTC 4 cut(s) 814, 1047, 1730, 1819
Psp5II RGGWCCY 2 cut(s) 413, 880
Psp6I CCWGG 1 cut(s) 664
PspGI CCWGG 1 cut(s) 664
PspLI CGTACG 1 cut(s) 1137
PspN4I GGNNCC 5 cut(s) 168, 270, 415, 882, 1612
PspPI GGNCC 4 cut(s) 269, 413, 880, 1383
PspPPI RGGWCCY 2 cut(s) 413, 880
PstI CTGCAG 1 cut(s) 1807
PsuI RGATCY 2 cut(s) 553, 595
RsaI GTAC 7 cut(s) 19, 41, 586, 743, 1139, 1301, 1677
RsaNI GTAC 7 cut(s) 18, 40, 585, 742, 1138, 1300, 1676
SacI GAGCTC 4 cut(s) 814, 1047, 1730, 1819
SalI GTCGAC 1 cut(s) 380
SapI GCTCTTC 1 cut(s) 1718
SaqAI TTAA 7 cut(s) 300, 654, 720, 1128, 1410, 1755, 1772
SatI GCNGC 7 cut(s) 360, 426, 1295, 1428, 1441, 1767, 1803
Sau3AI GATC 8 cut(s) 553, 595, 724, 840, 1000, 1261, 1401, 1545
Sau96I GGNCC 4 cut(s) 269, 413, 880, 1383
SchI GAGTC 3 cut(s) 86, 1094, 1664
ScrFI CCNGG 1 cut(s) 666
SduI GDGCHC 5 cut(s) 814, 1047, 1719, 1730, 1819
SfaNI GCATC 5 cut(s) 40, 46, 697, 893, 1283
SfcI CTRYAG 2 cut(s) 557, 1803
SinI GGWCC 4 cut(s) 269, 413, 880, 1383
SmlI CTYRAG 3 cut(s) 569, 1592, 1754
SmoI CTYRAG 3 cut(s) 569, 1592, 1754
SphI GCATGC 1 cut(s) 955
SseBI AGGCCT 1 cut(s) 538
SsiI CCGC 5 cut(s) 178, 884, 914, 1467, 1766
SspMI CTAG 4 cut(s) 618, 993, 1437, 1832
SstI GAGCTC 4 cut(s) 814, 1047, 1730, 1819
StuI AGGCCT 1 cut(s) 538
StyD4I CCNGG 1 cut(s) 664
StyI CCWWGG 1 cut(s) 1080
TaaI ACNGT 4 cut(s) 850, 945, 1323, 1673
TaiI ACGT 2 cut(s) 526, 1601
TaqI TCGA 3 cut(s) 80, 381, 999
TatI WGTACW 2 cut(s) 741, 1675
TauI GCSGC 1 cut(s) 1769
TfiI GAWTC 6 cut(s) 122, 364, 419, 470, 996, 1118
Tru1I TTAA 7 cut(s) 300, 654, 720, 1128, 1410, 1755, 1772
Tru9I TTAA 7 cut(s) 300, 654, 720, 1128, 1410, 1755, 1772
TscAI CASTG 4 cut(s) 403, 950, 1119, 1678
TseFI GTSAC 2 cut(s) 1086, 1207
TseI GCWGC 6 cut(s) 359, 425, 1294, 1427, 1440, 1802
Tsp45I GTSAC 2 cut(s) 1086, 1207
TspDTI ATGAA 5 cut(s) 525, 789, 845, 1351, 1551
TspGWI ACGGA 2 cut(s) 719, 1339
TspRI CASTG 4 cut(s) 403, 950, 1119, 1678
Van91I CCANNNNNTGG 1 cut(s) 1463
Vha464I CTTAAG 1 cut(s) 1754
VpaK11BI GGWCC 4 cut(s) 269, 413, 880, 1383
XagI CCTNNNNNAGG 1 cut(s) 1692
XapI RAATTY 4 cut(s) 638, 681, 1367, 1406
XceI RCATGY 4 cut(s) 955, 1187, 1456, 1582
XmiI GTMKAC 1 cut(s) 381
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 4 cut(s) 618, 993, 1437, 1832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.