pycom02g01660

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011250
Physical Location & Seq
Reverse (-)
986541 .. 990434
3894 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1428 bp
ATGTCGTCGGAGATCGAGGTCGTCGACGATGAGGTCCAAACCGGTGATCCTGGATCCGTCTCAGCCAATTCAACGAACAACAATGGCGACGCCAATCTGATTGGGGAAGAGAGTCTGCGAAACGACGTGTATACGGCGGCTGCGTATGGGGATTTGGAGAAGCTTCAGAGATTGGTGGAGTGTGAGGGTTGCTCTGTTTCCGAAACCGATGGTCTCGGCTACTATGCCCTCCAGTGGGCCGCTCTCAACAACCGCACTGCCGCTGCCCAGTATATTATTGAGCATGGAGGAGATGTGAATGCCACAGATCATACGGGGCAGACAGCATTACACTGGAGTGCGGTGCGGGGTGCAATCCAGGTTGCGGAGCTATTACTCCAAGAGGGTGCTCGGGTGAATGCTGTCGATATGTATGGCTATCAGACTACACATGTTGCGGCTCAATATGGTCAGACAGCTTTCCTGTACCATATTGTTTCAAAATGGAATGCTGACCCGGATGTGCCTGATAATGATGGGAGAAGCCCTTTACACTGGGCTGCTTATAAAGGTTTTGCTGATTGTATACGTCTTCTTTTATTTCTGGATGCATACAAAGGACGCCAAGATAAAGAGGGTTGCACTCCTTTGCATTGGGCTGCTATCAGGGGTAACTTGGAGGCATGTACAGTGTTGGTACAGGCTGGAAAGAAGGACGACTTAATGGTTACAGACAACACTGGCCTTACACCAGCACAACTTGCCGCTGATAAGAATCACAGACAAGTTGCCTTTTTCCTTGGGAATGCTAGAAGACTGCTTGACAAACGCTGTGATGGGAACAGTCATCTTGGAGGAATTTCAAAACTAGGACTTGCGCCACTACTTTGGTGTATAATCTTTGTGCTGCTGGTGACCTATACTCATTCGGTCATTATGGCACCAAGTCTGCCAAAATTAACAGCTGGATCAGTTTTTGTTGCATGGCTAGGAGTTTTCCTAGCAACATCCGGGCTTGTTATGTTCTATAGATGCAGCAGCAAGGATCCAGGTTACATCCGAATGAATGTCCATGATTCACAAAATATGAAAGATGATGAGCCTCTTTTGAAGATTGAGATAAATAATCCTGCCTTGCTAGCTGGTAATTGGTCTCAGCTCTGTGCAACGTGCAAGATTGTCAGGCCTCTTCGTGCAAAGCATTGTCCAACTTGTGATCGCTGTGTTGAACAATTTGACCACCATTGCCCCTGGGTATCCAATTGCATTGGCAAGAAAAACAAATGGGATTTCTTTGCTTTCCTTGTTCTGGAAGTTTTGGCGATGCTGTTTACTGGAGGGGTTACTCTTATGAGAGTTTCGAGCGATCCATTGTGTCCATCTTCTTTTGGAGCATGGATCGATCATTTGTTGCTACTAGACATGTGGGTGCTCTTTCATTTTTTATAA

Protein Analysis

476

Amino Acids

52.33

Weight (kDa)

5.63

Isoelectric Point (pI)

29.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 45 - 136 5.1e-16 Ankyrin repeats (3 copies)
Ank_4 PF13637 77 - 126 6.1e-08 Ankyrin repeats (many copies)
Ank PF00023 106 - 136 5e-06 Ankyrin repeat
Ank_2 PF12796 144 - 230 2.6e-16 Ankyrin repeats (3 copies)
Ank_2 PF12796 199 - 260 7.2e-09 Ankyrin repeats (3 copies)
DHHC PF01529 379 - 447 8.3e-24 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20350
fragaria_vesca FvH4_1g02030 FvH4_1g02030 FvH4_1g02030
malus_domestica MD02G1018500.v1.1 MD15G1162500.v1.1
prunus_persica Prupe.7G252300_v2.0.a1
pyrus_communis pycom02g01660 pycom15g14610
rosa_chinensis RchiOBHm_Chr2g0087091
rosa_laevigata RLG00000015831
rosa_multiflora Rmu_sc0005550.1_g000004
rosa_roxburghii Rroxscaffold_2G00153890 Rroxscaffold_3G00239100
rosa_rugosa Rorug01G0472400
rosa_samantha Rh2AG025700 Rh2BG025400 Rh2CG026000 Rh2DG025900
rosa_wichuraiana Rw2G002040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 546, 1426
AasI GACNNNNNNGTC 1 cut(s) 32
AccB1I GGYRCC 1 cut(s) 919
AccBSI CCGCTC 1 cut(s) 242
AccI GTMKAC 3 cut(s) 24, 131, 565
AciI CCGC 9 cut(s) 137, 240, 253, 261, 341, 346, 365, 437, 744
AclWI GGATC 8 cut(s) 41, 48, 61, 955, 1019, 1032, 1340, 1385
AcsI RAATTY 1 cut(s) 837
AcuI CTGAAG 1 cut(s) 149
AcyI GRCGYC 2 cut(s) 90, 601
AfaI GTAC 3 cut(s) 467, 667, 678
AfiI CCNNNNNNNGG 4 cut(s) 234, 235, 364, 1288
AflIII ACRYGT 3 cut(s) 126, 430, 1401
AgeI ACCGGT 1 cut(s) 41
AgsI TTSAA 5 cut(s) 72, 480, 843, 1090, 1208
AjiI CACGTC 1 cut(s) 127
AjnI CCWGG 4 cut(s) 49, 357, 1027, 1229
AleI CACNNNNGTG 1 cut(s) 336
AluBI AGCT 6 cut(s) 163, 370, 458, 944, 1121, 1138
AluI AGCT 6 cut(s) 163, 370, 458, 944, 1121, 1138
Alw21I GWGCWC 2 cut(s) 391, 1413
Alw26I GTCTC 3 cut(s) 64, 218, 1137
AlwI GGATC 8 cut(s) 41, 48, 61, 955, 1019, 1032, 1340, 1385
Ama87I CYCGRG 1 cut(s) 390
AoxI GGCC 3 cut(s) 237, 721, 1163
ApeKI GCWGC 7 cut(s) 140, 263, 539, 638, 886, 1014, 1017
ApoI RAATTY 1 cut(s) 837
AsiGI ACCGGT 1 cut(s) 41
AspLEI GCGC 1 cut(s) 859
AspS9I GGNCC 2 cut(s) 34, 237
AsuC2I CCSGG 2 cut(s) 497, 991
AsuHPI GGTGA 3 cut(s) 56, 406, 904
AsuNHI GCTAGC 1 cut(s) 1117
AvaI CYCGRG 1 cut(s) 390
AvaII GGWCC 1 cut(s) 34
BamHI GGATCC 2 cut(s) 53, 1024
BanI GGYRCC 1 cut(s) 919
BbsI GAAGAC 2 cut(s) 563, 799
Bbv12I GWGCWC 2 cut(s) 391, 1413
BbvI GCAGC 7 cut(s) 127, 250, 526, 625, 873, 1026, 1029
BccI CCATC 4 cut(s) 203, 509, 809, 1366
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 4 cut(s) 51, 359, 1029, 1231
BciVI GTATCC 1 cut(s) 1246
BcnI CCSGG 2 cut(s) 497, 991
BcoDI GTCTC 3 cut(s) 64, 218, 1137
BfaI CTAG 6 cut(s) 789, 848, 968, 980, 1118, 1397
BfmI CTRYAG 1 cut(s) 1006
BfuI GTATCC 1 cut(s) 1246
Bme1390I CCNGG 6 cut(s) 51, 359, 497, 991, 1029, 1231
Bme18I GGWCC 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 390
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 34, 237
BmiI GGNNCC 3 cut(s) 55, 921, 1026
BmrFI CCNGG 6 cut(s) 51, 359, 497, 991, 1029, 1231
BmrI ACTGGG 2 cut(s) 262, 544
BmsI GCATC 3 cut(s) 577, 1001, 1293
BmtI GCTAGC 1 cut(s) 1121
BmuI ACTGGG 2 cut(s) 262, 544
BpiI GAAGAC 2 cut(s) 563, 799
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpmI CTGGAG 3 cut(s) 215, 355, 1335
BpuMI CCSGG 2 cut(s) 497, 991
Bsa29I ATCGAT 1 cut(s) 1380
BsaBI GATNNNNATC 1 cut(s) 753
BsaHI GRCGYC 2 cut(s) 90, 601
BsaI GGTCTC 2 cut(s) 218, 1137
BsaJI CCNNGG 3 cut(s) 778, 1229, 1230
BsaWI WCCGGW 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 4 cut(s) 234, 235, 364, 1288
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 6 cut(s) 232, 268, 338, 539, 724, 1318
Bse3DI GCAATG 1 cut(s) 1222
Bse8I GATNNNNATC 1 cut(s) 753
BseBI CCWGG 4 cut(s) 51, 359, 1029, 1231
BseCI ATCGAT 1 cut(s) 1380
BseDI CCNNGG 3 cut(s) 778, 1229, 1230
BseGI GGATG 4 cut(s) 505, 592, 986, 1035
BseJI GATNNNNATC 1 cut(s) 753
BseLI CCNNNNNNNGG 4 cut(s) 234, 235, 364, 1288
BseMI GCAATG 1 cut(s) 1222
BseMII CTCAG 2 cut(s) 75, 1148
BseNI ACTGG 6 cut(s) 232, 268, 338, 539, 724, 1318
BseRI GAGGAG 1 cut(s) 303
BseXI GCAGC 7 cut(s) 127, 250, 526, 625, 873, 1026, 1029
BshFI GGCC 3 cut(s) 239, 723, 1165
BshNI GGYRCC 1 cut(s) 919
BshTI ACCGGT 1 cut(s) 41
BshVI ATCGAT 1 cut(s) 1380
BsiHKAI GWGCWC 2 cut(s) 391, 1413
BsiHKCI CYCGRG 1 cut(s) 390
BsiSI CCGG 3 cut(s) 42, 497, 990
BslI CCNNNNNNNGG 4 cut(s) 234, 235, 364, 1288
BsmAI GTCTC 3 cut(s) 64, 218, 1137
BsmBI CGTCTC 1 cut(s) 64
BsmI GAATGC 4 cut(s) 304, 403, 493, 790
BsnI GGCC 3 cut(s) 239, 723, 1165
Bso31I GGTCTC 2 cut(s) 218, 1137
BsoBI CYCGRG 1 cut(s) 390
Bsp1286I GDGCHC 2 cut(s) 391, 1413
Bsp1407I TGTACA 1 cut(s) 665
BspACI CCGC 9 cut(s) 137, 240, 253, 261, 341, 346, 365, 437, 744
BspANI GGCC 3 cut(s) 239, 723, 1165
BspCNI CTCAG 2 cut(s) 74, 1147
BspDI ATCGAT 1 cut(s) 1380
BspLI GGNNCC 3 cut(s) 55, 921, 1026
BspOI GCTAGC 1 cut(s) 1121
BspPI GGATC 8 cut(s) 41, 48, 61, 955, 1019, 1032, 1340, 1385
BspT107I GGYRCC 1 cut(s) 919
BspTNI GGTCTC 2 cut(s) 218, 1137
BsrBI CCGCTC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 1222
BsrFI RCCGGY 1 cut(s) 41
BsrGI TGTACA 1 cut(s) 665
BsrI ACTGG 6 cut(s) 232, 268, 338, 539, 724, 1318
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 3 cut(s) 778, 1229, 1230
BssNAI GTATAC 2 cut(s) 132, 566
BssNI GRCGYC 2 cut(s) 90, 601
BssT1I CCWWGG 1 cut(s) 778
Bst1107I GTATAC 2 cut(s) 132, 566
Bst2UI CCWGG 4 cut(s) 51, 359, 1029, 1231
Bst4CI ACNGT 2 cut(s) 670, 824
Bst6I CTCTTC 2 cut(s) 102, 1173
BstACI GRCGYC 2 cut(s) 90, 601
BstAPI GCANNNNNTGC 1 cut(s) 740
BstAUI TGTACA 1 cut(s) 665
BstC8I GCNNGC 1 cut(s) 1119
BstDEI CTNAG 2 cut(s) 61, 1134
BstEII GGTNACC 1 cut(s) 892
BstF5I GGATG 4 cut(s) 505, 592, 986, 1035
BstHHI GCGC 1 cut(s) 859
BstMAI GTCTC 3 cut(s) 64, 218, 1137
BstMWI GCNNNNNNNGC 2 cut(s) 740, 1118
BstNI CCWGG 4 cut(s) 51, 359, 1029, 1231
BstNSI RCATGY 3 cut(s) 434, 666, 1405
BstPI GGTNACC 1 cut(s) 892
BstSCI CCNGG 6 cut(s) 49, 357, 495, 989, 1027, 1229
BstSFI CTRYAG 1 cut(s) 1006
BstV1I GCAGC 7 cut(s) 127, 250, 526, 625, 873, 1026, 1029
BstV2I GAAGAC 2 cut(s) 563, 799
BstX2I RGATCY 2 cut(s) 53, 1024
BstXI CCANNNNNNTGG 1 cut(s) 867
BstYI RGATCY 2 cut(s) 53, 1024
BstZ17I GTATAC 2 cut(s) 132, 566
Bsu15I ATCGAT 1 cut(s) 1380
BsuI GTATCC 1 cut(s) 1246
BsuRI GGCC 3 cut(s) 239, 723, 1165
BsuTUI ATCGAT 1 cut(s) 1380
BtgZI GCGATG 1 cut(s) 1316
BtrI CACGTC 1 cut(s) 127
BtsCI GGATG 4 cut(s) 505, 592, 986, 1035
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 6 cut(s) 239, 255, 331, 532, 675, 717
Cac8I GCNNGC 1 cut(s) 1119
CfoI GCGC 1 cut(s) 859
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 2 cut(s) 34, 237
ClaI ATCGAT 1 cut(s) 1380
CseI GACGC 2 cut(s) 98, 609
Csp6I GTAC 3 cut(s) 466, 666, 677
CspAI ACCGGT 1 cut(s) 41
CviAII CATG 7 cut(s) 284, 431, 663, 963, 1052, 1374, 1402
CviQI GTAC 3 cut(s) 466, 666, 677
DdeI CTNAG 2 cut(s) 61, 1134
DrdI GACNNNNNNGTC 1 cut(s) 32
DseDI GACNNNNNNGTC 1 cut(s) 32
Eam1104I CTCTTC 2 cut(s) 102, 1173
EarI CTCTTC 2 cut(s) 102, 1173
Eco130I CCWWGG 1 cut(s) 778
Eco147I AGGCCT 1 cut(s) 1165
Eco31I GGTCTC 2 cut(s) 218, 1137
Eco47I GGWCC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 149
Eco88I CYCGRG 1 cut(s) 390
Eco91I GGTNACC 1 cut(s) 892
EcoO65I GGTNACC 1 cut(s) 892
EcoRII CCWGG 4 cut(s) 49, 357, 1027, 1229
EcoT14I CCWWGG 1 cut(s) 778
EcoT22I ATGCAT 1 cut(s) 592
ErhI CCWWGG 1 cut(s) 778
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 7 cut(s) 287, 434, 666, 966, 1055, 1377, 1405
FalI AAGNNNNNCTT 2 cut(s) 683, 715
FatI CATG 7 cut(s) 283, 430, 662, 962, 1051, 1373, 1401
FauI CCCGC 1 cut(s) 339
FblI GTMKAC 3 cut(s) 24, 131, 565
FokI GGATG 4 cut(s) 512, 599, 973, 1022
FspBI CTAG 6 cut(s) 789, 848, 968, 980, 1118, 1397
GlaI GCGC 1 cut(s) 858
GsuI CTGGAG 3 cut(s) 215, 355, 1335
HaeIII GGCC 3 cut(s) 239, 723, 1165
HapII CCGG 3 cut(s) 42, 497, 990
HgaI GACGC 2 cut(s) 98, 609
HhaI GCGC 1 cut(s) 859
Hin1I GRCGYC 2 cut(s) 90, 601
Hin1II CATG 7 cut(s) 287, 434, 666, 966, 1055, 1377, 1405
Hin6I GCGC 1 cut(s) 857
HinP1I GCGC 1 cut(s) 857
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 3 cut(s) 112, 754, 1055
HpaII CCGG 3 cut(s) 42, 497, 990
HphI GGTGA 3 cut(s) 56, 406, 904
Hpy166II GTNNAC 4 cut(s) 25, 132, 566, 1311
Hpy188I TCNGA 7 cut(s) 10, 99, 168, 202, 423, 453, 1040
Hpy188III TCNNGA 2 cut(s) 584, 1289
Hpy8I GTNNAC 4 cut(s) 25, 132, 566, 1311
Hpy99I CGWCG 5 cut(s) 10, 26, 29, 92, 128
HpyAV CCTTC 1 cut(s) 685
HpyCH4III ACNGT 2 cut(s) 670, 824
HpyCH4IV ACGT 3 cut(s) 126, 568, 1148
HpyF10VI GCNNNNNNNGC 2 cut(s) 740, 1118
HpyF3I CTNAG 2 cut(s) 61, 1134
HpySE526I ACGT 3 cut(s) 126, 568, 1148
Hsp92I GRCGYC 2 cut(s) 90, 601
Hsp92II CATG 7 cut(s) 287, 434, 666, 966, 1055, 1377, 1405
HspAI GCGC 1 cut(s) 857
LmnI GCTCC 2 cut(s) 367, 1370
Lsp1109I GCAGC 7 cut(s) 127, 250, 526, 625, 873, 1026, 1029
LweI GCATC 3 cut(s) 577, 1001, 1293
MaeI CTAG 6 cut(s) 789, 848, 968, 980, 1118, 1397
MaeII ACGT 3 cut(s) 126, 568, 1148
MaeIII GTNAC 5 cut(s) 650, 706, 892, 1031, 1321
MbiI CCGCTC 1 cut(s) 242
MboII GAAGA 6 cut(s) 119, 563, 804, 1102, 1160, 1353
MfeI CAATTG 1 cut(s) 1240
MflI RGATCY 2 cut(s) 53, 1024
MhlI GDGCHC 2 cut(s) 391, 1413
MluCI AATT 6 cut(s) 67, 837, 935, 1126, 1211, 1240
MlyI GAGTC 1 cut(s) 121
MmeI TCCRAC 1 cut(s) 1211
Mph1103I ATGCAT 1 cut(s) 592
MseI TTAA 2 cut(s) 701, 938
MslI CAYNNNNRTG 3 cut(s) 336, 1040, 1406
MspA1I CMGCKG 3 cut(s) 263, 746, 944
MspI CCGG 3 cut(s) 42, 497, 990
MspR9I CCNGG 6 cut(s) 51, 359, 497, 991, 1029, 1231
MunI CAATTG 1 cut(s) 1240
Mva1269I GAATGC 4 cut(s) 304, 403, 493, 790
MvaI CCWGG 4 cut(s) 51, 359, 1029, 1231
MwoI GCNNNNNNNGC 2 cut(s) 740, 1118
NciI CCSGG 2 cut(s) 497, 991
NheI GCTAGC 1 cut(s) 1117
NlaIII CATG 7 cut(s) 287, 434, 666, 966, 1055, 1377, 1405
NlaIV GGNNCC 3 cut(s) 55, 921, 1026
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 1 cut(s) 892
NsiI ATGCAT 1 cut(s) 592
NspI RCATGY 3 cut(s) 434, 666, 1405
OliI CACNNNNGTG 1 cut(s) 336
PasI CCCWGGG 1 cut(s) 1230
PceI AGGCCT 1 cut(s) 1165
PciI ACATGT 2 cut(s) 430, 1401
PcsI WCGNNNNNNNCGW 2 cut(s) 21, 140
PctI GAATGC 4 cut(s) 304, 403, 493, 790
PfeI GAWTC 2 cut(s) 754, 1055
PfoI TCCNGGA 1 cut(s) 49
PinAI ACCGGT 1 cut(s) 41
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
PscI ACATGT 2 cut(s) 430, 1401
PsiI TTATAA 2 cut(s) 546, 1426
Psp6I CCWGG 4 cut(s) 49, 357, 1027, 1229
PspEI GGTNACC 1 cut(s) 892
PspGI CCWGG 4 cut(s) 49, 357, 1027, 1229
PspN4I GGNNCC 3 cut(s) 55, 921, 1026
PspPI GGNCC 2 cut(s) 34, 237
PsuI RGATCY 2 cut(s) 53, 1024
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 3 cut(s) 467, 667, 678
RsaNI GTAC 3 cut(s) 466, 666, 677
RseI CAYNNNNRTG 3 cut(s) 336, 1040, 1406
SalI GTCGAC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 701, 938
Sau96I GGNCC 2 cut(s) 34, 237
SchI GAGTC 1 cut(s) 121
ScrFI CCNGG 6 cut(s) 51, 359, 497, 991, 1029, 1231
SduI GDGCHC 2 cut(s) 391, 1413
SfaNI GCATC 3 cut(s) 577, 1001, 1293
SfcI CTRYAG 1 cut(s) 1006
SgrDI CGTCGACG 1 cut(s) 23
SinI GGWCC 1 cut(s) 34
SmiMI CAYNNNNRTG 3 cut(s) 336, 1040, 1406
Sse9I AATT 6 cut(s) 67, 837, 935, 1126, 1211, 1240
SseBI AGGCCT 1 cut(s) 1165
SsiI CCGC 9 cut(s) 137, 240, 253, 261, 341, 346, 365, 437, 744
SspMI CTAG 6 cut(s) 789, 848, 968, 980, 1118, 1397
StuI AGGCCT 1 cut(s) 1165
StyD4I CCNGG 6 cut(s) 49, 357, 495, 989, 1027, 1229
StyI CCWWGG 1 cut(s) 778
TaaI ACNGT 2 cut(s) 670, 824
TaiI ACGT 3 cut(s) 129, 571, 1151
TaqI TCGA 5 cut(s) 15, 24, 405, 1340, 1380
TaqII GACCGA 1 cut(s) 898
TasI AATT 6 cut(s) 67, 837, 935, 1126, 1211, 1240
TatI WGTACW 1 cut(s) 665
TauI GCSGC 5 cut(s) 140, 242, 263, 440, 746
TfiI GAWTC 2 cut(s) 754, 1055
Tru1I TTAA 2 cut(s) 701, 938
Tru9I TTAA 2 cut(s) 701, 938
TscAI CASTG 6 cut(s) 239, 262, 338, 539, 675, 724
TseFI GTSAC 1 cut(s) 892
TseI GCWGC 7 cut(s) 140, 263, 539, 638, 886, 1014, 1017
Tsp45I GTSAC 1 cut(s) 892
TspDTI ATGAA 3 cut(s) 1058, 1082, 1406
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 6 cut(s) 239, 262, 338, 539, 675, 724
VpaK11BI GGWCC 1 cut(s) 34
XapI RAATTY 1 cut(s) 837
XceI RCATGY 3 cut(s) 434, 666, 1405
XmiI GTMKAC 3 cut(s) 24, 131, 565
XspI CTAG 6 cut(s) 789, 848, 968, 980, 1118, 1397
Zsp2I ATGCAT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.