pycom02g02160

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0009084
Physical Location & Seq
Forward (+)
1282726 .. 1284850
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2040 bp
ATGGCGTGCTTCCGGTTCAAGCTGTGGTGGATGGCCCAGAAGATGGGAGATCAGGGAAGGGACATTCCTCTTGAGACTCAATTCCTATTGGTCGAGACCAAGCACGGGTCCCACCTGGAATCGGACGGCGGAGACGAAGAGAACCAGATCGTCTACACCGTGTTCCTCCCTCTCATCGAAGGCTCGTTCCGCGGTTGCCTGCAAGGCAACGCGCGTGACGAGCTCGAGCTCTGTTTGGAGAGCGGCGACGCCGACACCAAAGCGTCATCGTTTTCCCACGCGCTGCACGTCCACGCCGGGACGGACCCGTTCGGTACGATAACGGAAGCCATCAGGTCCGTCAAAGTTCACCTTCAGACGTTTCGTCAACGTCACGAGAAGAAACTCCCCGGAATCGTTGACTATTTCGGGTGGTGCACCTGGGACGCGTTTTACCGGGACGTGACTCAGGAGGACGTGGAGGCCGGGCTCGAGTCTTTAGCCGCCGGTGGAACACCTCCGAAGTTCGTTATCATCGACGATGGGTGGCAGTCTGTAGGCGGCGACGACGGCGTCGAAAAGCAGGAGCCGCTAAGGCTGACCGGGATCAAAGAAAATTCAAAGTTTCAGAAGAAGGACGATCCGACAGTGGGGATTAAGAACATCGTCAACATCGCGAAGCAAAAGCACGGGTTGAAGTATGTGTACGTGTGGCACGCGATTACTGGCTACTGGGGCGGGGTCAGACCCGGAATGAAAGAAATGGAGGAATTCGGATCCTTGTTGAAGTACCCGAACGTTTCCAGCGGTGTTGTGGCGAACGAACCGACATGGAAGACGGACGCGATGGCGGTGCGAGGGTTGGGCCTGGTGGATCCAAAGAGCGTGTACAAATTCTACAACGAATTGCACAGCTACCTGAGCTCTGCGGGTATTGACGGCGTTAAGGTGGACGTGCAGTGCATACTGGAAACTCTCGGAGCCGGGTTAGGCGGTCGGGTCGAGTTGACCCAGAAGTACCACCAGGCGCTGGACGCCTCGGTGGCGAGGAATTTCCCGGACAACGGCTGCATCGCGTGCATGAGCCACAATACTGACGCGCTCTACTGCTCCAAACAGACCGCCGTCGTGAGGGCGTCCGACGATTTCTACCCCCGCGACCCGGTGTCGCACACGATCCACATTGCGGCGGTTGCTTACAACAGCGTATTTCTCGGTGAGTTTATGCTGCCGGACTGGGACATGTTCCACTCGCAGCATCCGGCGGCTGAGTACCACGCCTCCGCCAGGGCCATCAGTGGTGGGCCCATCTATGTCAGTGACGCGCCTGGGAAGCACAATTTCGAGCTGCTAAGGAAGCTGGTGTTGTCGGACGGGTCGGTGCTTCGGGCCCGATTTCCCGGACGCCCGACGAAAGACTGCCTGTTCTCTGACCCGGCCCGCGACGGCGTGAGCTTGCTGAAGATATGGAACATGAACAAGTACACCGGAGTCCTCGGCGTCTACAACTGCCAAGGCGCGGCGTGGAGCACCGCGGAACGGAAGAACACGTTCCACGAAACCAAATCCGAGGCCATCACGGGTCTGATTCGCGGCCGCGACGTCCACCTGATCGCGGAGGCTGCCGTGGACAACGACTGGAAAGGCGACTGCGCCGTGTACAGTCACCGGATGGGAGAGCTGGTGACTCTGCCTTACAATGCGTCGATGCCGATTTCGCTCAAAGTGCTGGAGCACGAGGTTTTCACGGTGACTCCGATCAGGATTTTGGGCGGCAGATTGAGTTTCGCGCCAATTGGACTGGTGGAGATGTACAATGCAGGCGGAGCAATTGTAGGGTTGCGATATGAACAGAACGCCATAGTCCGATTGGAGGTTAAGGGCTGCGGCAAATTTGGCGCGTACTCTTCTGCCAAGCCCAAGAAGTGTCGGGTCGGGTCTAATGTGGTGGATTTTGAGTACGACTCGTCGTCTGGGTTGGTGATTTTGAGATTGGATCATTTGCCTGAGGAGGGTCAGCAAGTTCATGTCGTTGAGATTGAATTATTAGAAAGAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

680

Amino Acids

75.06

Weight (kDa)

5.96

Isoelectric Point (pI)

31.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 1 - 657 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 551
AatII GACGTC 1 cut(s) 1584
AccB7I CCANNNNNTGG 2 cut(s) 43, 1009
AccBSI CCGCTC 1 cut(s) 243
AccI GTMKAC 2 cut(s) 153, 1482
AclI AACGTT 1 cut(s) 777
AclWI GGATC 8 cut(s) 593, 614, 750, 763, 848, 861, 1150, 1983
AcoI YGGCCR 1 cut(s) 1573
AcsI RAATTY 5 cut(s) 595, 749, 872, 1030, 1871
AcuI CTGAAG 2 cut(s) 338, 1460
AcyI GRCGYC 7 cut(s) 249, 552, 1014, 1115, 1384, 1479, 1581
AflIII ACRYGT 4 cut(s) 426, 687, 1221, 1527
AgsI TTSAA 5 cut(s) 19, 600, 676, 766, 2021
AhdI GACNNNNNGTC 3 cut(s) 363, 1144, 1948
AjiI CACGTC 4 cut(s) 289, 442, 457, 934
AjnI CCWGG 6 cut(s) 114, 419, 846, 1002, 1265, 1306
AluBI AGCT 9 cut(s) 22, 223, 229, 894, 903, 1327, 1339, 1434, 1660
AluI AGCT 9 cut(s) 22, 223, 229, 894, 903, 1327, 1339, 1434, 1660
Alw21I GWGCWC 6 cut(s) 225, 231, 419, 905, 1511, 1716
Alw26I GTCTC 3 cut(s) 68, 89, 126
Alw44I GTGCAC 1 cut(s) 415
AlwI GGATC 8 cut(s) 593, 614, 750, 763, 848, 861, 1150, 1983
AlwNI CAGNNNCTG 1 cut(s) 1009
Ama87I CYCGRG 2 cut(s) 224, 470
AoxI GGCC 9 cut(s) 33, 462, 844, 1269, 1283, 1368, 1416, 1551, 1573
ApaI GGGCCC 2 cut(s) 1287, 1372
ApaLI GTGCAC 1 cut(s) 415
ApeKI GCWGC 7 cut(s) 283, 1047, 1207, 1234, 1327, 1601, 1863
ApoI RAATTY 5 cut(s) 595, 749, 872, 1030, 1871
Asp700I GAANNNNTTC 1 cut(s) 1529
AspLEI GCGC 9 cut(s) 214, 283, 1009, 1081, 1306, 1499, 1634, 1771, 1880
AsuHPI GGTGA 6 cut(s) 341, 1208, 1637, 1675, 1741, 1972
AvaI CYCGRG 2 cut(s) 224, 470
AvaII GGWCC 3 cut(s) 108, 304, 336
AxyI CCTNAGG 1 cut(s) 1986
BaeGI GKGCMC 3 cut(s) 419, 1287, 1372
BamHI GGATCC 2 cut(s) 755, 853
BanII GRGCYC 6 cut(s) 225, 231, 471, 905, 1287, 1372
BarI GAAGNNNNNNTAC 2 cut(s) 668, 700
BauI CACGAG 2 cut(s) 374, 1715
BbsI GAAGAC 1 cut(s) 821
Bbv12I GWGCWC 6 cut(s) 225, 231, 419, 905, 1511, 1716
BbvI GCAGC 7 cut(s) 270, 1034, 1194, 1246, 1314, 1588, 1850
BccI CCATC 9 cut(s) 25, 37, 338, 515, 820, 1280, 1295, 1562, 1645
BceAI ACGGC 8 cut(s) 142, 565, 934, 1060, 1088, 1441, 1589, 1619
BcgI CGANNNNNNTGC 2 cut(s) 814, 848
BciT130I CCWGG 6 cut(s) 116, 421, 848, 1004, 1267, 1308
BcoDI GTCTC 3 cut(s) 68, 89, 126
BfmI CTRYAG 1 cut(s) 534
BfoI RGCGCY 1 cut(s) 1010
BglI GCCNNNNNGGC 3 cut(s) 204, 574, 1022
Bme18I GGWCC 3 cut(s) 108, 304, 336
BmeRI GACNNNNNGTC 3 cut(s) 363, 1144, 1948
BmeT110I CYCGRG 2 cut(s) 224, 470
BmgBI CACGTC 4 cut(s) 289, 442, 457, 934
BmiI GGNNCC 9 cut(s) 109, 110, 306, 567, 757, 855, 961, 1285, 1370
BmrI ACTGGG 2 cut(s) 721, 1225
BmsI GCATC 3 cut(s) 1059, 1246, 1677
BmuI ACTGGG 2 cut(s) 721, 1225
BoxI GACNNNNGTC 1 cut(s) 1103
BpiI GAAGAC 1 cut(s) 821
BplI GAGNNNNNCTC 2 cut(s) 1928, 1960
BpmI CTGGAG 1 cut(s) 1730
Bpu10I CCTNAGC 3 cut(s) 572, 899, 1331
BpuEI CTTGAG 1 cut(s) 92
BsaAI YACGTR 1 cut(s) 688
BsaHI GRCGYC 7 cut(s) 249, 552, 1014, 1115, 1384, 1479, 1581
BsaI GGTCTC 1 cut(s) 89
BsaWI WCCGGW 3 cut(s) 12, 1466, 1647
BsaXI ACNNNNNCTCC 8 cut(s) 1244, 1274, 1647, 1677, 1717, 1747, 1797, 1827
Bse118I RCCGGY 1 cut(s) 485
Bse1I ACTGG 6 cut(s) 709, 716, 951, 1220, 1622, 1785
Bse21I CCTNAGG 1 cut(s) 1986
Bse3DI GCAATG 1 cut(s) 1161
BseBI CCWGG 6 cut(s) 116, 421, 848, 1004, 1267, 1308
BseGI GGATG 3 cut(s) 36, 1237, 1656
BseMI GCAATG 1 cut(s) 1161
BseMII CTCAG 4 cut(s) 461, 890, 1239, 1977
BseNI ACTGG 6 cut(s) 709, 716, 951, 1220, 1622, 1785
BseRI GAGGAG 1 cut(s) 2003
BseSI GKGCMC 3 cut(s) 419, 1287, 1372
BseX3I CGGCCG 1 cut(s) 1573
BseXI GCAGC 7 cut(s) 270, 1034, 1194, 1246, 1314, 1588, 1850
BsgI GTGCAG 2 cut(s) 269, 956
Bsh1285I CGRYCG 2 cut(s) 976, 1576
BshFI GGCC 9 cut(s) 35, 464, 846, 1271, 1285, 1370, 1418, 1553, 1575
BsiEI CGRYCG 2 cut(s) 976, 1576
BsiHKAI GWGCWC 6 cut(s) 225, 231, 419, 905, 1511, 1716
BsiHKCI CYCGRG 2 cut(s) 224, 470
BslFI GGGAC 6 cut(s) 74, 94, 313, 437, 452, 1232
BsmAI GTCTC 3 cut(s) 68, 89, 126
BsmBI CGTCTC 1 cut(s) 126
BsmFI GGGAC 6 cut(s) 74, 94, 313, 437, 452, 1232
BsnI GGCC 9 cut(s) 35, 464, 846, 1271, 1285, 1370, 1418, 1553, 1575
Bso31I GGTCTC 1 cut(s) 89
BsoBI CYCGRG 2 cut(s) 224, 470
Bsp120I GGGCCC 2 cut(s) 1283, 1368
Bsp1286I GDGCHC 9 cut(s) 225, 231, 419, 471, 905, 1287, 1372, 1511, 1716
Bsp1407I TGTACA 3 cut(s) 867, 1638, 1791
Bsp68I TCGCGA 1 cut(s) 656
BspANI GGCC 9 cut(s) 35, 464, 846, 1271, 1285, 1370, 1418, 1553, 1575
BspCNI CTCAG 4 cut(s) 460, 891, 1240, 1978
BspLI GGNNCC 9 cut(s) 109, 110, 306, 567, 757, 855, 961, 1285, 1370
BspPI GGATC 8 cut(s) 593, 614, 750, 763, 848, 861, 1150, 1983
BspTNI GGTCTC 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 1161
BsrFI RCCGGY 1 cut(s) 485
BsrGI TGTACA 3 cut(s) 867, 1638, 1791
BsrI ACTGG 6 cut(s) 709, 716, 951, 1220, 1622, 1785
BssAI RCCGGY 1 cut(s) 485
BssNI GRCGYC 7 cut(s) 249, 552, 1014, 1115, 1384, 1479, 1581
BssSI CACGAG 2 cut(s) 374, 1715
BssT1I CCWWGG 1 cut(s) 1492
Bst2BI CACGAG 2 cut(s) 374, 1715
Bst2UI CCWGG 6 cut(s) 116, 421, 848, 1004, 1267, 1308
Bst4CI ACNGT 4 cut(s) 160, 628, 1643, 1729
Bst6I CTCTTC 2 cut(s) 132, 1891
BstACI GRCGYC 7 cut(s) 249, 552, 1014, 1115, 1384, 1479, 1581
BstAPI GCANNNNNTGC 1 cut(s) 1056
BstAUI TGTACA 3 cut(s) 867, 1638, 1791
BstBAI YACGTR 1 cut(s) 688
BstC8I GCNNGC 7 cut(s) 7, 200, 696, 1057, 1420, 1436, 1801
BstDEI CTNAG 6 cut(s) 447, 572, 899, 1248, 1331, 1986
BstDSI CCRYGG 3 cut(s) 190, 1512, 1605
BstENI CCTNNNNNAGG 1 cut(s) 1264
BstF5I GGATG 3 cut(s) 36, 1237, 1656
BstH2I RGCGCY 1 cut(s) 1010
BstHHI GCGC 9 cut(s) 214, 283, 1009, 1081, 1306, 1499, 1634, 1771, 1880
BstMAI GTCTC 3 cut(s) 68, 89, 126
BstMCI CGRYCG 2 cut(s) 976, 1576
BstNI CCWGG 6 cut(s) 116, 421, 848, 1004, 1267, 1308
BstNSI RCATGY 1 cut(s) 1225
BstPAI GACNNNNGTC 1 cut(s) 1103
BstSFI CTRYAG 1 cut(s) 534
BstSLI GKGCMC 3 cut(s) 419, 1287, 1372
BstV1I GCAGC 7 cut(s) 270, 1034, 1194, 1246, 1314, 1588, 1850
BstV2I GAAGAC 1 cut(s) 821
BstX2I RGATCY 2 cut(s) 755, 853
BstYI RGATCY 2 cut(s) 755, 853
BstZI CGGCCG 1 cut(s) 1573
Bsu36I CCTNAGG 1 cut(s) 1986
BsuRI GGCC 9 cut(s) 35, 464, 846, 1271, 1285, 1370, 1418, 1553, 1575
BtgI CCRYGG 3 cut(s) 190, 1512, 1605
BtgZI GCGATG 3 cut(s) 637, 839, 1036
BtrI CACGTC 4 cut(s) 289, 442, 457, 934
BtsCI GGATG 3 cut(s) 36, 1237, 1656
BtsI GCAGTG 1 cut(s) 944
BtsIMutI CAGTG 4 cut(s) 633, 944, 1282, 1303
BtuMI TCGCGA 1 cut(s) 656
Cac8I GCNNGC 7 cut(s) 7, 200, 696, 1057, 1420, 1436, 1801
CaiI CAGNNNCTG 1 cut(s) 1009
CciNI GCGGCCGC 1 cut(s) 1573
CfoI GCGC 9 cut(s) 214, 283, 1009, 1081, 1306, 1499, 1634, 1771, 1880
Cfr10I RCCGGY 1 cut(s) 485
Cfr42I CCGCGG 2 cut(s) 193, 1515
CviAII CATG 5 cut(s) 810, 1060, 1222, 1453, 2006
DdeI CTNAG 6 cut(s) 447, 572, 899, 1248, 1331, 1986
DrdI GACNNNNNNGTC 1 cut(s) 551
DriI GACNNNNNGTC 3 cut(s) 363, 1144, 1948
DseDI GACNNNNNNGTC 1 cut(s) 551
EaeI YGGCCR 1 cut(s) 1573
EagI CGGCCG 1 cut(s) 1573
Eam1104I CTCTTC 2 cut(s) 132, 1891
Eam1105I GACNNNNNGTC 3 cut(s) 363, 1144, 1948
EarI CTCTTC 2 cut(s) 132, 1891
EciI GGCGGA 3 cut(s) 144, 1252, 1818
Ecl136II GAGCTC 3 cut(s) 223, 229, 903
EclXI CGGCCG 1 cut(s) 1573
Eco130I CCWWGG 1 cut(s) 1492
Eco24I GRGCYC 6 cut(s) 225, 231, 471, 905, 1287, 1372
Eco31I GGTCTC 1 cut(s) 89
Eco47I GGWCC 3 cut(s) 108, 304, 336
Eco52I CGGCCG 1 cut(s) 1573
Eco53kI GAGCTC 3 cut(s) 223, 229, 903
Eco57I CTGAAG 2 cut(s) 338, 1460
Eco81I CCTNAGG 1 cut(s) 1986
Eco88I CYCGRG 2 cut(s) 224, 470
EcoICRI GAGCTC 3 cut(s) 223, 229, 903
EcoNI CCTNNNNNAGG 1 cut(s) 1264
EcoO109I RGGNCCY 1 cut(s) 108
EcoRI GAATTC 1 cut(s) 749
EcoRII CCWGG 6 cut(s) 114, 419, 846, 1002, 1265, 1306
EcoT14I CCWWGG 1 cut(s) 1492
EcoT38I GRGCYC 6 cut(s) 225, 231, 471, 905, 1287, 1372
ErhI CCWWGG 1 cut(s) 1492
Esp3I CGTCTC 1 cut(s) 126
FaeI CATG 5 cut(s) 813, 1063, 1225, 1456, 2009
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 6 cut(s) 74, 94, 313, 437, 452, 1232
FatI CATG 5 cut(s) 809, 1059, 1221, 1452, 2005
FauI CCCGC 4 cut(s) 710, 901, 1142, 1427
FblI GTMKAC 2 cut(s) 153, 1482
FokI GGATG 3 cut(s) 43, 1224, 1663
FriOI GRGCYC 6 cut(s) 225, 231, 471, 905, 1287, 1372
GlaI GCGC 9 cut(s) 213, 282, 1008, 1080, 1305, 1498, 1633, 1770, 1879
GsuI CTGGAG 1 cut(s) 1730
HaeII RGCGCY 1 cut(s) 1010
HaeIII GGCC 9 cut(s) 35, 464, 846, 1271, 1285, 1370, 1418, 1553, 1575
HhaI GCGC 9 cut(s) 214, 283, 1009, 1081, 1306, 1499, 1634, 1771, 1880
Hin1I GRCGYC 7 cut(s) 249, 552, 1014, 1115, 1384, 1479, 1581
Hin1II CATG 5 cut(s) 813, 1063, 1225, 1456, 2009
Hin6I GCGC 9 cut(s) 212, 281, 1007, 1079, 1304, 1497, 1632, 1769, 1878
HinP1I GCGC 9 cut(s) 212, 281, 1007, 1079, 1304, 1497, 1632, 1769, 1878
HincII GTYRAC 4 cut(s) 368, 400, 649, 987
HindII GTYRAC 4 cut(s) 368, 400, 649, 987
HphI GGTGA 6 cut(s) 341, 1208, 1637, 1675, 1741, 1972
Hpy188III TCNNGA 7 cut(s) 71, 94, 374, 449, 655, 1108, 1741
HpyAV CCTTC 4 cut(s) 51, 173, 362, 607
HpyCH4III ACNGT 4 cut(s) 160, 628, 1643, 1729
HpyCH4V TGCA 9 cut(s) 202, 286, 417, 889, 937, 942, 1050, 1059, 1799
HpyF3I CTNAG 6 cut(s) 447, 572, 899, 1248, 1331, 1986
Hsp92I GRCGYC 7 cut(s) 249, 552, 1014, 1115, 1384, 1479, 1581
Hsp92II CATG 5 cut(s) 813, 1063, 1225, 1456, 2009
HspAI GCGC 9 cut(s) 212, 281, 1007, 1079, 1304, 1497, 1632, 1769, 1878
KflI GGGWCCC 1 cut(s) 108
KspI CCGCGG 2 cut(s) 193, 1515
LmnI GCTCC 6 cut(s) 565, 959, 1094, 1506, 1711, 1805
Lsp1109I GCAGC 7 cut(s) 270, 1034, 1194, 1246, 1314, 1588, 1850
LweI GCATC 3 cut(s) 1059, 1246, 1677
MaeIII GTNAC 7 cut(s) 215, 371, 442, 1298, 1643, 1663, 1729
MbiI CCGCTC 1 cut(s) 243
MboII GAAGA 8 cut(s) 52, 149, 391, 622, 826, 1453, 1534, 1878
MfeI CAATTG 2 cut(s) 1773, 1809
MflI RGATCY 2 cut(s) 755, 853
MhlI GDGCHC 9 cut(s) 225, 231, 419, 471, 905, 1287, 1372, 1511, 1716
MluI ACGCGT 1 cut(s) 426
MlyI GAGTC 7 cut(s) 70, 439, 482, 1479, 1660, 1726, 1937
MmeI TCCRAC 3 cut(s) 647, 1143, 1329
MroXI GAANNNNTTC 1 cut(s) 1529
MseI TTAA 3 cut(s) 636, 924, 1857
MspA1I CMGCKG 3 cut(s) 192, 786, 1514
MunI CAATTG 2 cut(s) 1773, 1809
MvaI CCWGG 6 cut(s) 116, 421, 848, 1004, 1267, 1308
NlaIII CATG 5 cut(s) 813, 1063, 1225, 1456, 2009
NlaIV GGNNCC 9 cut(s) 109, 110, 306, 567, 757, 855, 961, 1285, 1370
NmeAIII GCCGAG 1 cut(s) 1455
NmuCI GTSAC 7 cut(s) 215, 371, 442, 1298, 1643, 1663, 1729
NotI GCGGCCGC 1 cut(s) 1573
NruI TCGCGA 1 cut(s) 656
NspI RCATGY 1 cut(s) 1225
PaeR7I CTCGAG 2 cut(s) 224, 470
PciI ACATGT 1 cut(s) 1221
PcsI WCGNNNNNNNCGW 7 cut(s) 132, 156, 216, 285, 513, 552, 1733
PdmI GAANNNNTTC 1 cut(s) 1529
PfeI GAWTC 3 cut(s) 119, 393, 1567
PflFI GACNNNGTC 1 cut(s) 551
PflMI CCANNNNNTGG 2 cut(s) 43, 1009
PfoI TCCNGGA 2 cut(s) 1035, 1378
PleI GAGTC 7 cut(s) 70, 439, 481, 1478, 1660, 1726, 1937
PpsI GAGTC 7 cut(s) 70, 439, 481, 1478, 1660, 1726, 1937
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 108
PscI ACATGT 1 cut(s) 1221
PshAI GACNNNNGTC 1 cut(s) 1103
Psp124BI GAGCTC 3 cut(s) 225, 231, 905
Psp1406I AACGTT 1 cut(s) 777
Psp5II RGGWCCY 1 cut(s) 108
Psp6I CCWGG 6 cut(s) 114, 419, 846, 1002, 1265, 1306
PspGI CCWGG 6 cut(s) 114, 419, 846, 1002, 1265, 1306
PspN4I GGNNCC 9 cut(s) 109, 110, 306, 567, 757, 855, 961, 1285, 1370
PspOMI GGGCCC 2 cut(s) 1283, 1368
PspPPI RGGWCCY 1 cut(s) 108
PspXI VCTCGAGB 2 cut(s) 224, 470
PstNI CAGNNNCTG 1 cut(s) 1009
PsuI RGATCY 2 cut(s) 755, 853
PsyI GACNNNGTC 1 cut(s) 551
RruI TCGCGA 1 cut(s) 656
SacI GAGCTC 3 cut(s) 225, 231, 905
SacII CCGCGG 2 cut(s) 193, 1515
SaqAI TTAA 3 cut(s) 636, 924, 1857
SchI GAGTC 7 cut(s) 70, 439, 482, 1479, 1660, 1726, 1937
SduI GDGCHC 9 cut(s) 225, 231, 419, 471, 905, 1287, 1372, 1511, 1716
SfaNI GCATC 3 cut(s) 1059, 1246, 1677
SfcI CTRYAG 1 cut(s) 534
Sfr274I CTCGAG 2 cut(s) 224, 470
Sfr303I CCGCGG 2 cut(s) 193, 1515
SgrAI CRCCGGYG 1 cut(s) 485
SgrBI CCGCGG 2 cut(s) 193, 1515
SinI GGWCC 3 cut(s) 108, 304, 336
SlaI CTCGAG 2 cut(s) 224, 470
SmlI CTYRAG 3 cut(s) 71, 224, 470
SmoI CTYRAG 3 cut(s) 71, 224, 470
SstI GAGCTC 3 cut(s) 225, 231, 905
StyI CCWWGG 1 cut(s) 1492
TaaI ACNGT 4 cut(s) 160, 628, 1643, 1729
TaqI TCGA 9 cut(s) 93, 177, 225, 471, 516, 555, 981, 1323, 1685
TatI WGTACW 4 cut(s) 867, 1461, 1638, 1791
TfiI GAWTC 3 cut(s) 119, 393, 1567
Tru1I TTAA 3 cut(s) 636, 924, 1857
Tru9I TTAA 3 cut(s) 636, 924, 1857
TscAI CASTG 4 cut(s) 633, 944, 1282, 1303
TseFI GTSAC 7 cut(s) 215, 371, 442, 1298, 1643, 1663, 1729
TseI GCWGC 7 cut(s) 283, 1047, 1207, 1234, 1327, 1601, 1863
Tsp45I GTSAC 7 cut(s) 215, 371, 442, 1298, 1643, 1663, 1729
TspDTI ATGAA 4 cut(s) 749, 1469, 1842, 1994
TspGWI ACGGA 5 cut(s) 317, 328, 338, 833, 1534
TspRI CASTG 4 cut(s) 633, 944, 1282, 1303
Tth111I GACNNNGTC 1 cut(s) 551
Van91I CCANNNNNTGG 2 cut(s) 43, 1009
VneI GTGCAC 1 cut(s) 415
VpaK11BI GGWCC 3 cut(s) 108, 304, 336
XagI CCTNNNNNAGG 1 cut(s) 1264
XapI RAATTY 5 cut(s) 595, 749, 872, 1030, 1871
XceI RCATGY 1 cut(s) 1225
XcmI CCANNNNNNNNNTGG 2 cut(s) 790, 1846
XhoI CTCGAG 2 cut(s) 224, 470
XmiI GTMKAC 2 cut(s) 153, 1482
XmnI GAANNNNTTC 1 cut(s) 1529
ZraI GACGTC 1 cut(s) 1582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.