pycom02g06200

Glutamate-rich WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011879
Physical Location & Seq
Reverse (-)
4239851 .. 4241531
1681 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 834 bp
ATGACACAAAATCCACATATATGTGCATCTTGGGCAGACACCGGTCATGTGCAGGTTTGGGACTTCAGTTCCCATTTGAATGCATTAGCGGAGTCGGAGACTGAAGCTAGCCAGGGAGCTTCTTCCGTTTTTAACCAGGCTCCATTGGTTAAGTTTGGGCATAAAGATGAAGGGTTTGCTCTAGACTGGAGTCCGCTTGTACCTGGACGGTTTCTATCTGGGGACTGCAAGAATTGTATACATTTATGGGAACCTACATCTGGTTCAACATGGACTGTTAATACTACTCCATATATTGGACATATTGCAAGCGTGGAGGATCTACAATGGATTCCTACAGAACCTGATGTATTTGCTTCTTGTTCTGTTGATGGGAATATAGCAATATGGGATATCCGCATAGGGAAGGCACCAGCAACTTCTTTTAAGGCGCATAATGCAGATGTGAATGTTATGTCTTGGAATAGGCTGACTAGTTGTATGCTGGCATCTGGAAGTGACGATGGCACCTTTTCCATTCGCGATCTTAGATTGCTGAAGGAAGGAGATTCTGCGGTGGCGCATTTTGAATACCATAAACACCCTATCACATCCATCGAGTGGAGTCCACACGAAGCCTCCACTTTGGCCGTGACGTCTTCTGACAATCAGCTGACAATATGGGATCTATCTTTAGAAAATGACAGGGAGGAAGAGGCAGAATTCAAAGCTAAGACAAAAGAGGAAGTGAATGCTCCCAAATATTTACCTCCGCAGCTTCTGTTTGTTCATCAGGGACAGAAAGACTTGAAAGAACTTCATTGGCATACTCAGATTCCTGGCATGCTCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAF1C_H4-bd WD40

Protein Analysis

278

Amino Acids

30.8

Weight (kDa)

5.05

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_Aladin PF25460 46 - 165 4e-06 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 54 - 154 6.6e-13 THOC3 beta-propeller domain
WD40_Prp19 PF24814 69 - 177 3.7e-07 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 75 - 262 1.1e-20 CDC20/Fizzy WD40 domain
Beta-prop_EIPR1 PF23609 76 - 171 1.8e-07 EIPR1 beta-propeller
Beta-prop_WDR3_1st PF25173 78 - 227 1.3e-16 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 81 - 229 2.6e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 101 - 224 2.8e-16 WDR5 beta-propeller domain
Beta-prop_WDR19_1st PF23389 103 - 224 7.9e-06 WDR19 first beta-propeller
Beta-prop_EML_2 PF23414 111 - 227 2e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 114 - 224 1.7e-07 TEP-1 second beta-propeller
WDR55 PF24796 118 - 225 2.1e-09 WDR55
Beta-prop_WDR36-Utp21_1st PF25171 125 - 259 1.1e-07 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 140 - 269 9.1e-12 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19540
fragaria_vesca FvH4_1g07300
malus_domestica MD02G1077800.v1.1 MD15G1206400.v1.1
prunus_persica Prupe.7G211000_v2.0.a1 Prupe.7G211000_v2.0.a1 Prupe.7G211000_v2.0.a1
pyrus_communis pycom02g06200
rosa_chinensis RchiOBHm_Chr2g0093231
rosa_laevigata RLG00000016380
rosa_multiflora Rmu_sc0003724.1_g000014 Rmu_sc0037462.1_g000001
rosa_roxburghii Rroxscaffold_2G00148390
rosa_rugosa Rorug02G0033700
rosa_samantha Rh2AG079900 Rh2BG080800 Rh2CG083200 Rh2DG078800
rosa_wichuraiana Rw2G006710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 638
Acc36I ACCTGC 1 cut(s) 43
AccB1I GGYRCC 2 cut(s) 409, 506
AccB7I CCANNNNNTGG 2 cut(s) 296, 600
AccI GTMKAC 1 cut(s) 238
AccII CGCG 1 cut(s) 522
AciI CCGC 5 cut(s) 89, 194, 397, 554, 752
AclWI GGATC 2 cut(s) 327, 672
AcoI YGGCCR 1 cut(s) 627
AcsI RAATTY 1 cut(s) 701
AcuI CTGAAG 3 cut(s) 49, 123, 557
AcyI GRCGYC 1 cut(s) 635
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 3 cut(s) 260, 296, 600
AgeI ACCGGT 1 cut(s) 41
AgsI TTSAA 5 cut(s) 79, 267, 569, 706, 790
AhlI ACTAGT 1 cut(s) 473
AjnI CCWGG 4 cut(s) 111, 135, 202, 817
AluBI AGCT 5 cut(s) 107, 119, 652, 710, 757
AluI AGCT 5 cut(s) 107, 119, 652, 710, 757
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 2 cut(s) 327, 672
AlwNI CAGNNNCTG 2 cut(s) 344, 760
AoxI GGCC 1 cut(s) 627
ApeKI GCWGC 1 cut(s) 754
ApoI RAATTY 1 cut(s) 701
AsiGI ACCGGT 1 cut(s) 41
AspLEI GCGC 2 cut(s) 433, 562
AsuNHI GCTAGC 1 cut(s) 107
BanI GGYRCC 2 cut(s) 409, 506
BbsI GAAGAC 1 cut(s) 630
BbvI GCAGC 1 cut(s) 766
BccI CCATC 3 cut(s) 365, 497, 602
BceAI ACGGC 1 cut(s) 614
BciT130I CCWGG 4 cut(s) 113, 137, 204, 819
BcoDI GTCTC 1 cut(s) 92
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 3 cut(s) 108, 182, 474
BfmI CTRYAG 1 cut(s) 336
BfuAI ACCTGC 1 cut(s) 43
BisI GCNGC 1 cut(s) 755
BlsI GCNGC 1 cut(s) 756
Bme1390I CCNGG 4 cut(s) 113, 137, 204, 819
BmiI GGNNCC 4 cut(s) 141, 252, 411, 508
BmrFI CCNGG 4 cut(s) 113, 137, 204, 819
BmsI GCATC 2 cut(s) 35, 497
BmtI GCTAGC 1 cut(s) 111
BoxI GACNNNNGTC 2 cut(s) 42, 189
BpiI GAAGAC 1 cut(s) 630
BpmI CTGGAG 1 cut(s) 208
BsaHI GRCGYC 1 cut(s) 635
BsaJI CCNNGG 1 cut(s) 112
BsaWI WCCGGW 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 602, 632
Bsc4I CCNNNNNNNGG 3 cut(s) 260, 296, 600
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 191
BseBI CCWGG 4 cut(s) 113, 137, 204, 819
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 1 cut(s) 590
BseLI CCNNNNNNNGG 3 cut(s) 260, 296, 600
BseMII CTCAG 1 cut(s) 824
BseNI ACTGG 1 cut(s) 191
BseXI GCAGC 1 cut(s) 766
BsgI GTGCAG 1 cut(s) 71
Bsh1236I CGCG 1 cut(s) 522
BshFI GGCC 1 cut(s) 629
BshNI GGYRCC 2 cut(s) 409, 506
BshTI ACCGGT 1 cut(s) 41
BsiSI CCGG 1 cut(s) 42
BslFI GGGAC 3 cut(s) 74, 236, 789
BslI CCNNNNNNNGG 3 cut(s) 260, 296, 600
BsmAI GTCTC 1 cut(s) 92
BsmFI GGGAC 3 cut(s) 74, 236, 789
BsmI GAATGC 2 cut(s) 85, 736
BsnI GGCC 1 cut(s) 629
Bsp143I GATC 3 cut(s) 319, 523, 664
Bsp68I TCGCGA 1 cut(s) 522
BspACI CCGC 5 cut(s) 89, 194, 397, 554, 752
BspANI GGCC 1 cut(s) 629
BspCNI CTCAG 1 cut(s) 823
BspFNI CGCG 1 cut(s) 522
BspLI GGNNCC 4 cut(s) 141, 252, 411, 508
BspMI ACCTGC 1 cut(s) 43
BspOI GCTAGC 1 cut(s) 111
BspPI GGATC 2 cut(s) 327, 672
BspT107I GGYRCC 2 cut(s) 409, 506
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 3 cut(s) 319, 523, 664
BssNAI GTATAC 1 cut(s) 239
BssNI GRCGYC 1 cut(s) 635
Bst1107I GTATAC 1 cut(s) 239
Bst2UI CCWGG 4 cut(s) 113, 137, 204, 819
Bst4CI ACNGT 2 cut(s) 210, 277
Bst6I CTCTTC 1 cut(s) 687
BstACI GRCGYC 1 cut(s) 635
BstC8I GCNNGC 4 cut(s) 109, 310, 486, 824
BstDEI CTNAG 3 cut(s) 527, 711, 810
BstF5I GGATG 1 cut(s) 590
BstFNI CGCG 1 cut(s) 522
BstHHI GCGC 2 cut(s) 433, 562
BstKTI GATC 3 cut(s) 322, 526, 667
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 3 cut(s) 319, 523, 664
BstMWI GCNNNNNNNGC 2 cut(s) 32, 437
BstNI CCWGG 4 cut(s) 113, 137, 204, 819
BstNSI RCATGY 1 cut(s) 826
BstPAI GACNNNNGTC 2 cut(s) 42, 189
BstSCI CCNGG 4 cut(s) 111, 135, 202, 817
BstSFI CTRYAG 1 cut(s) 336
BstUI CGCG 1 cut(s) 522
BstV1I GCAGC 1 cut(s) 766
BstV2I GAAGAC 1 cut(s) 630
BstX2I RGATCY 2 cut(s) 319, 664
BstYI RGATCY 2 cut(s) 319, 664
BstZ17I GTATAC 1 cut(s) 239
BsuRI GGCC 1 cut(s) 629
BtsCI GGATG 1 cut(s) 590
BtuMI TCGCGA 1 cut(s) 522
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 4 cut(s) 109, 310, 486, 824
CaiI CAGNNNCTG 2 cut(s) 344, 760
CfoI GCGC 2 cut(s) 433, 562
Cfr10I RCCGGY 1 cut(s) 41
Csp6I GTAC 1 cut(s) 200
CspAI ACCGGT 1 cut(s) 41
CviAII CATG 3 cut(s) 47, 270, 823
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 3 cut(s) 527, 711, 810
DpnI GATC 3 cut(s) 321, 525, 666
DpnII GATC 3 cut(s) 319, 523, 664
EaeI YGGCCR 1 cut(s) 627
Eam1104I CTCTTC 1 cut(s) 687
EarI CTCTTC 1 cut(s) 687
Eco32I GATATC 1 cut(s) 394
Eco57I CTGAAG 3 cut(s) 49, 123, 557
EcoRI GAATTC 1 cut(s) 701
EcoRII CCWGG 4 cut(s) 111, 135, 202, 817
EcoRV GATATC 1 cut(s) 394
EcoT22I ATGCAT 1 cut(s) 85
FaeI CATG 3 cut(s) 50, 273, 826
FaqI GGGAC 3 cut(s) 74, 236, 789
FatI CATG 3 cut(s) 46, 269, 822
FblI GTMKAC 1 cut(s) 238
Fnu4HI GCNGC 1 cut(s) 755
FokI GGATG 1 cut(s) 577
Fsp4HI GCNGC 1 cut(s) 755
FspBI CTAG 3 cut(s) 108, 182, 474
GlaI GCGC 2 cut(s) 432, 561
GluI GCNGC 1 cut(s) 755
GsuI CTGGAG 1 cut(s) 208
HaeIII GGCC 1 cut(s) 629
HapII CCGG 1 cut(s) 42
HhaI GCGC 2 cut(s) 433, 562
Hin1I GRCGYC 1 cut(s) 635
Hin1II CATG 3 cut(s) 50, 273, 826
Hin6I GCGC 2 cut(s) 431, 560
HinP1I GCGC 2 cut(s) 431, 560
HinfI GANTC 6 cut(s) 92, 190, 331, 548, 604, 814
HpaII CCGG 1 cut(s) 42
Hpy166II GTNNAC 2 cut(s) 239, 608
Hpy188I TCNGA 3 cut(s) 97, 643, 813
Hpy188III TCNNGA 3 cut(s) 182, 492, 521
Hpy8I GTNNAC 2 cut(s) 239, 608
HpyAV CCTTC 4 cut(s) 164, 400, 532, 536
HpyCH4III ACNGT 2 cut(s) 210, 277
HpyCH4IV ACGT 1 cut(s) 635
HpyCH4V TGCA 6 cut(s) 26, 52, 83, 228, 308, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 437
HpyF3I CTNAG 3 cut(s) 527, 711, 810
HpySE526I ACGT 1 cut(s) 635
Hsp92I GRCGYC 1 cut(s) 635
Hsp92II CATG 3 cut(s) 50, 273, 826
HspAI GCGC 2 cut(s) 431, 560
Kzo9I GATC 3 cut(s) 319, 523, 664
LmnI GCTCC 3 cut(s) 116, 145, 739
Lsp1109I GCAGC 1 cut(s) 766
LweI GCATC 2 cut(s) 35, 497
MaeI CTAG 3 cut(s) 108, 182, 474
MaeII ACGT 1 cut(s) 635
MaeIII GTNAC 2 cut(s) 497, 631
MalI GATC 3 cut(s) 321, 525, 666
MboI GATC 3 cut(s) 319, 523, 664
MboII GAAGA 3 cut(s) 114, 630, 704
MflI RGATCY 2 cut(s) 319, 664
MluCI AATT 2 cut(s) 232, 701
MlyI GAGTC 3 cut(s) 101, 199, 613
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 6 cut(s) 310, 628, 682, 688, 715, 759
Mph1103I ATGCAT 1 cut(s) 85
MseI TTAA 4 cut(s) 132, 150, 279, 426
MslI CAYNNNNRTG 4 cut(s) 19, 21, 78, 165
MspA1I CMGCKG 1 cut(s) 652
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 4 cut(s) 113, 137, 204, 819
Mva1269I GAATGC 2 cut(s) 85, 736
MvaI CCWGG 4 cut(s) 113, 137, 204, 819
MvnI CGCG 1 cut(s) 522
MwoI GCNNNNNNNGC 2 cut(s) 32, 437
NdeII GATC 3 cut(s) 319, 523, 664
NheI GCTAGC 1 cut(s) 107
NlaIII CATG 3 cut(s) 50, 273, 826
NlaIV GGNNCC 4 cut(s) 141, 252, 411, 508
NmuCI GTSAC 2 cut(s) 497, 631
NruI TCGCGA 1 cut(s) 522
NsiI ATGCAT 1 cut(s) 85
NspI RCATGY 1 cut(s) 826
PaeI GCATGC 1 cut(s) 826
PctI GAATGC 2 cut(s) 85, 736
PfeI GAWTC 3 cut(s) 331, 548, 814
PflMI CCANNNNNTGG 2 cut(s) 296, 600
PinAI ACCGGT 1 cut(s) 41
PkrI GCNGC 1 cut(s) 756
PleI GAGTC 3 cut(s) 100, 198, 612
PpsI GAGTC 3 cut(s) 100, 198, 612
PshAI GACNNNNGTC 2 cut(s) 42, 189
Psp6I CCWGG 4 cut(s) 111, 135, 202, 817
PspGI CCWGG 4 cut(s) 111, 135, 202, 817
PspN4I GGNNCC 4 cut(s) 141, 252, 411, 508
PstNI CAGNNNCTG 2 cut(s) 344, 760
PsuI RGATCY 2 cut(s) 319, 664
PvuII CAGCTG 1 cut(s) 652
RruI TCGCGA 1 cut(s) 522
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
RseI CAYNNNNRTG 4 cut(s) 19, 21, 78, 165
SaqAI TTAA 4 cut(s) 132, 150, 279, 426
SatI GCNGC 1 cut(s) 755
Sau3AI GATC 3 cut(s) 319, 523, 664
SchI GAGTC 3 cut(s) 101, 199, 613
ScrFI CCNGG 4 cut(s) 113, 137, 204, 819
SfaNI GCATC 2 cut(s) 35, 497
SfcI CTRYAG 1 cut(s) 336
SmiMI CAYNNNNRTG 4 cut(s) 19, 21, 78, 165
SpeI ACTAGT 1 cut(s) 473
SphI GCATGC 1 cut(s) 826
Sse9I AATT 2 cut(s) 232, 701
SsiI CCGC 5 cut(s) 89, 194, 397, 554, 752
SspI AATATT 1 cut(s) 743
SspMI CTAG 3 cut(s) 108, 182, 474
StyD4I CCNGG 4 cut(s) 111, 135, 202, 817
TaaI ACNGT 2 cut(s) 210, 277
TaiI ACGT 1 cut(s) 638
TaqI TCGA 1 cut(s) 597
TasI AATT 2 cut(s) 232, 701
TfiI GAWTC 3 cut(s) 331, 548, 814
Tru1I TTAA 4 cut(s) 132, 150, 279, 426
Tru9I TTAA 4 cut(s) 132, 150, 279, 426
TseFI GTSAC 2 cut(s) 497, 631
TseI GCWGC 1 cut(s) 754
Tsp45I GTSAC 2 cut(s) 497, 631
TspDTI ATGAA 3 cut(s) 183, 758, 788
TspGWI ACGGA 1 cut(s) 115
Van91I CCANNNNNTGG 2 cut(s) 296, 600
XapI RAATTY 1 cut(s) 701
XbaI TCTAGA 1 cut(s) 181
XceI RCATGY 1 cut(s) 826
XmiI GTMKAC 1 cut(s) 238
XspI CTAG 3 cut(s) 108, 182, 474
ZraI GACGTC 1 cut(s) 636
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.