Rh2CG083200

Glutamate-rich WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0011879
Physical Location & Seq
Reverse (-)
6767187 .. 6770316
3130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG083200.1

Sequence Viewer

Length: 1173 bp
ATGGTTCGAAGCATTAAGAACCCTAAGAAAGCCAAAAGAAAGAACAAGGGCTCCAAGAAGGGAGATGGGTCTTCTTCGTCTTCAGGGCCATCAATGCCAGCGAAGGTGTGGCAGCCAGGTGTGGACAAATTGGAGGAAGGAGAAGAGCTCCAGTGTGACCCTTCAGCTTACAACTCTCTTCATGCCTTCCATATTGGTTGGCCTTGTCTAAGCTTTGATATTGTACGAGATACATTGGGGTTGGTGAGGACAGAGTTCCCTCATACGGTGTACTTTGTTGCTGGGACTCAGGCAGAGAAAGCTTCTTGGAACTCTCTTAGCATATTTAAGGTATCCAACATTTCTGGGAAAAAGCGCGAACCAGTGCCAACTAAATCTGGAGATGATGAGTCGAATATGGATAGTGATAGTGACGAAGATAGTGATGAGGAGGAGCTTGAAGGATCTGGCGCACCAATTTTGCAGTTGCGCAAGGTAACTCATGAAGGATGTGTGAATCGTATACGTGCCATGACACAAAGTCCTCATATATGTGCATCTTGGGGAGATACTGGTCATGTGCAGGTCTGGGACTTCAGTTCTCATCTGAATGCTTTAGCAGAATCAGAATCAGAAGTTAGTCAAGGACCTTCTAACCAAGCTCCAGTGTTTAAGTTTGGGCATAAAGATGAAGGATTCGCTCTCGACTGGAGTCCTCTTGTAACTGGAAGGCTTCTATCTGGGGACTGCAAGAGTAATATACATTTGTGGGAACCTACATCTGGTGGAACATGGAATGTTGATACCACTCCGTATATTGGGCATATTGCAAGCGTGGAAGATCTGCAGTGGAGCCCTACAGAAGCTGATGTTTTTGCCTCTTGTTCCGTGGATGGGAATATAGCAATATGGGATACCCGTTTAGGGAAGTCACCTGCAGCTTCTTTTAAGGCGCATAATGCAGATGTGAATGTTATTTCTTGGAACAGGCTGGCTAGCTGTATGTTAGCATCCGGAAGTGATGATGGTACCTTTTCCATCCGTGACCTTAGATTGCTTAAGGGACAAAAAGACTTGAAAGAACTTCATTGGCATTCACAAATTCCTGGCATGATCGTATCAACTGCAGCAGATGGTTATAATATTCTGATGCCTTCAAACATACAGACCACCCTTCCCTCCGATGGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAF1C_H4-bd WD40

Protein Analysis

390

Amino Acids

42.45

Weight (kDa)

5.38

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1C_H4-bd PF12265 48 - 114 2e-13 Histone-binding protein RBBP4 or subunit C of CAF1 complex
Beta-prop_WDR5 PF25175 160 - 303 5.1e-07 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 161 - 339 3.5e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 221 - 353 1.1e-16 THOC3 beta-propeller domain
WD40_Prp19 PF24814 228 - 345 3.4e-08 Prp19 WD40 domain
Beta-prop_EIPR1 PF23609 243 - 338 1.1e-06 EIPR1 beta-propeller
Beta-prop_WDR3_1st PF25173 245 - 352 5.3e-09 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 260 - 345 3.2e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 267 - 359 3.9e-11 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 267 - 368 2.5e-11 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011879)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19540
fragaria_vesca FvH4_1g07300
malus_domestica MD02G1077800.v1.1 MD15G1206400.v1.1
prunus_persica Prupe.7G211000_v2.0.a1 Prupe.7G211000_v2.0.a1 Prupe.7G211000_v2.0.a1
pyrus_communis pycom02g06200
rosa_chinensis RchiOBHm_Chr2g0093231
rosa_laevigata RLG00000016380
rosa_multiflora Rmu_sc0003724.1_g000014 Rmu_sc0037462.1_g000001
rosa_roxburghii Rroxscaffold_2G00148390
rosa_rugosa Rorug02G0033700
rosa_samantha Rh2AG079900 Rh2BG080800 Rh2CG083200 Rh2DG078800
rosa_wichuraiana Rw2G006710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1119
AarI CACCTGC 1 cut(s) 922
Acc16I TGCGCA 1 cut(s) 470
Acc36I ACCTGC 2 cut(s) 553, 922
Acc65I GGTACC 1 cut(s) 1007
AccB1I GGYRCC 1 cut(s) 1007
AccI GTMKAC 1 cut(s) 502
AccII CGCG 1 cut(s) 357
AccIII TCCGGA 1 cut(s) 992
AclWI GGATC 1 cut(s) 451
AcsI RAATTY 1 cut(s) 1080
AcuI CTGAAG 3 cut(s) 66, 147, 559
AfaI GTAC 3 cut(s) 225, 272, 1009
AfiI CCNNNNNNNGG 6 cut(s) 265, 761, 797, 873, 903, 1163
AflII CTTAAG 1 cut(s) 1037
AgsI TTSAA 3 cut(s) 440, 1057, 1137
AhdI GACNNNNNGTC 1 cut(s) 519
AjnI CCWGG 2 cut(s) 115, 1084
AloI GAACNNNNNNTCC 2 cut(s) 35, 67
AluBI AGCT 9 cut(s) 148, 167, 213, 302, 436, 641, 845, 920, 978
AluI AGCT 9 cut(s) 148, 167, 213, 302, 436, 641, 845, 920, 978
Alw21I GWGCWC 1 cut(s) 150
AlwI GGATC 1 cut(s) 451
AlwNI CAGNNNCTG 1 cut(s) 845
Aor13HI TCCGGA 1 cut(s) 992
AoxI GGCC 2 cut(s) 86, 200
ApeKI GCWGC 3 cut(s) 112, 917, 1106
ApoI RAATTY 1 cut(s) 1080
Asp718I GGTACC 1 cut(s) 1007
AspLEI GCGC 4 cut(s) 357, 452, 471, 934
AspS9I GGNCC 3 cut(s) 86, 626, 1166
AsuHPI GGTGA 2 cut(s) 256, 903
AsuII TTCGAA 1 cut(s) 7
AsuNHI GCTAGC 1 cut(s) 974
AvaII GGWCC 2 cut(s) 626, 1166
BanI GGYRCC 1 cut(s) 1007
BanII GRGCYC 3 cut(s) 53, 150, 836
BbsI GAAGAC 2 cut(s) 63, 72
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 3 cut(s) 124, 929, 1118
BccI CCATC 7 cut(s) 59, 97, 866, 998, 1025, 1106, 1157
BciT130I CCWGG 2 cut(s) 117, 1086
BciVI GTATCC 2 cut(s) 343, 886
BfaI CTAG 1 cut(s) 975
BfmI CTRYAG 4 cut(s) 824, 837, 915, 1104
BfrI CTTAAG 1 cut(s) 1037
BfuAI ACCTGC 2 cut(s) 553, 922
BfuI GTATCC 2 cut(s) 343, 886
BglII AGATCT 1 cut(s) 820
BisI GCNGC 3 cut(s) 113, 918, 1107
BlsI GCNGC 3 cut(s) 114, 919, 1108
Bme1390I CCNGG 2 cut(s) 117, 1086
Bme18I GGWCC 2 cut(s) 626, 1166
BmeRI GACNNNNNGTC 1 cut(s) 519
BmgT120I GGNCC 3 cut(s) 86, 626, 1166
BmiI GGNNCC 4 cut(s) 52, 753, 833, 1009
BmrFI CCNGG 2 cut(s) 117, 1086
BmsI GCATC 3 cut(s) 545, 998, 1119
BmtI GCTAGC 1 cut(s) 978
BoxI GACNNNNGTC 1 cut(s) 690
BpiI GAAGAC 2 cut(s) 63, 72
BplI GAGNNNNNCTC 2 cut(s) 132, 164
BpmI CTGGAG 4 cut(s) 134, 399, 627, 709
Bpu14I TTCGAA 1 cut(s) 7
BsaAI YACGTR 1 cut(s) 506
BsaJI CCNNGG 1 cut(s) 867
BsaWI WCCGGW 1 cut(s) 992
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 6 cut(s) 265, 761, 797, 873, 903, 1163
Bse1I ACTGG 6 cut(s) 151, 362, 556, 644, 692, 709
BseAI TCCGGA 1 cut(s) 992
BseBI CCWGG 2 cut(s) 117, 1086
BseDI CCNNGG 1 cut(s) 867
BseGI GGATG 4 cut(s) 494, 877, 989, 1017
BseLI CCNNNNNNNGG 6 cut(s) 265, 761, 797, 873, 903, 1163
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 6 cut(s) 151, 362, 556, 644, 692, 709
BseRI GAGGAG 2 cut(s) 443, 446
BseXI GCAGC 3 cut(s) 124, 929, 1118
BseYI CCCAGC 1 cut(s) 281
BsgI GTGCAG 1 cut(s) 581
Bsh1236I CGCG 1 cut(s) 357
BshFI GGCC 2 cut(s) 88, 202
BshNI GGYRCC 1 cut(s) 1007
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 1 cut(s) 993
BslFI GGGAC 4 cut(s) 298, 584, 737, 1056
BslI CCNNNNNNNGG 6 cut(s) 265, 761, 797, 873, 903, 1163
BsmFI GGGAC 4 cut(s) 298, 584, 737, 1056
BsmI GAATGC 2 cut(s) 595, 1072
BsnI GGCC 2 cut(s) 88, 202
Bsp119I TTCGAA 1 cut(s) 7
Bsp1286I GDGCHC 3 cut(s) 53, 150, 836
Bsp13I TCCGGA 1 cut(s) 992
Bsp143I GATC 3 cut(s) 443, 820, 1092
BspANI GGCC 2 cut(s) 88, 202
BspCNI CTCAG 1 cut(s) 301
BspEI TCCGGA 1 cut(s) 992
BspFNI CGCG 1 cut(s) 357
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 4 cut(s) 52, 753, 833, 1009
BspMAI CTGCAG 3 cut(s) 828, 919, 1108
BspMI ACCTGC 2 cut(s) 553, 922
BspOI GCTAGC 1 cut(s) 978
BspPI GGATC 1 cut(s) 451
BspQI GCTCTTC 1 cut(s) 138
BspT104I TTCGAA 1 cut(s) 7
BspT107I GGYRCC 1 cut(s) 1007
BspTI CTTAAG 1 cut(s) 1037
BsrI ACTGG 6 cut(s) 151, 362, 556, 644, 692, 709
BssECI CCNNGG 1 cut(s) 867
BssMI GATC 3 cut(s) 443, 820, 1092
BssNAI GTATAC 1 cut(s) 503
Bst1107I GTATAC 1 cut(s) 503
Bst2UI CCWGG 2 cut(s) 117, 1086
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 2 cut(s) 138, 183
BstAFI CTTAAG 1 cut(s) 1037
BstBAI YACGTR 1 cut(s) 506
BstBI TTCGAA 1 cut(s) 7
BstC8I GCNNGC 4 cut(s) 99, 811, 972, 976
BstDEI CTNAG 5 cut(s) 24, 209, 288, 317, 1028
BstDSI CCRYGG 1 cut(s) 867
BstF5I GGATG 4 cut(s) 494, 877, 989, 1017
BstFNI CGCG 1 cut(s) 357
BstHHI GCGC 4 cut(s) 357, 452, 471, 934
BstKTI GATC 3 cut(s) 446, 823, 1095
BstMBI GATC 3 cut(s) 443, 820, 1092
BstMWI GCNNNNNNNGC 3 cut(s) 94, 299, 938
BstNI CCWGG 2 cut(s) 117, 1086
BstPAI GACNNNNGTC 1 cut(s) 690
BstSCI CCNGG 2 cut(s) 115, 1084
BstSFI CTRYAG 4 cut(s) 824, 837, 915, 1104
BstUI CGCG 1 cut(s) 357
BstV1I GCAGC 3 cut(s) 124, 929, 1118
BstV2I GAAGAC 2 cut(s) 63, 72
BstX2I RGATCY 2 cut(s) 443, 820
BstYI RGATCY 2 cut(s) 443, 820
BstZ17I GTATAC 1 cut(s) 503
BsuI GTATCC 2 cut(s) 343, 886
BsuRI GGCC 2 cut(s) 88, 202
BtgI CCRYGG 1 cut(s) 867
BtsCI GGATG 4 cut(s) 494, 877, 989, 1017
BtsI GCAGTG 1 cut(s) 833
BtsIMutI CAGTG 4 cut(s) 158, 369, 651, 833
BveI ACCTGC 2 cut(s) 553, 922
Cac8I GCNNGC 4 cut(s) 99, 811, 972, 976
CaiI CAGNNNCTG 1 cut(s) 845
CciI TCATGA 1 cut(s) 481
CfoI GCGC 4 cut(s) 357, 452, 471, 934
Cfr13I GGNCC 3 cut(s) 86, 626, 1166
Csp6I GTAC 3 cut(s) 224, 271, 1008
CviAII CATG 6 cut(s) 182, 482, 511, 557, 771, 1090
CviQI GTAC 3 cut(s) 224, 271, 1008
DdeI CTNAG 5 cut(s) 24, 209, 288, 317, 1028
DpnI GATC 3 cut(s) 445, 822, 1094
DpnII GATC 3 cut(s) 443, 820, 1092
DriI GACNNNNNGTC 1 cut(s) 519
Eam1104I CTCTTC 2 cut(s) 138, 183
Eam1105I GACNNNNNGTC 1 cut(s) 519
EarI CTCTTC 2 cut(s) 138, 183
Ecl136II GAGCTC 1 cut(s) 148
Eco24I GRGCYC 3 cut(s) 53, 150, 836
Eco47I GGWCC 2 cut(s) 626, 1166
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 3 cut(s) 66, 147, 559
EcoICRI GAGCTC 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 626
EcoRII CCWGG 2 cut(s) 115, 1084
EcoT38I GRGCYC 3 cut(s) 53, 150, 836
FaeI CATG 6 cut(s) 185, 485, 514, 560, 774, 1093
FaqI GGGAC 4 cut(s) 298, 584, 737, 1056
FatI CATG 6 cut(s) 181, 481, 510, 556, 770, 1089
FblI GTMKAC 1 cut(s) 502
Fnu4HI GCNGC 3 cut(s) 113, 918, 1107
FokI GGATG 4 cut(s) 501, 884, 976, 1004
FriOI GRGCYC 3 cut(s) 53, 150, 836
Fsp4HI GCNGC 3 cut(s) 113, 918, 1107
FspBI CTAG 1 cut(s) 975
FspI TGCGCA 1 cut(s) 470
GlaI GCGC 4 cut(s) 356, 451, 470, 933
GluI GCNGC 3 cut(s) 113, 918, 1107
GsaI CCCAGC 1 cut(s) 285
GsuI CTGGAG 4 cut(s) 134, 399, 627, 709
HaeIII GGCC 2 cut(s) 88, 202
HapII CCGG 1 cut(s) 993
HhaI GCGC 4 cut(s) 357, 452, 471, 934
Hin1II CATG 6 cut(s) 185, 485, 514, 560, 774, 1093
Hin6I GCGC 4 cut(s) 355, 450, 469, 932
HinP1I GCGC 4 cut(s) 355, 450, 469, 932
HindIII AAGCTT 2 cut(s) 211, 300
HinfI GANTC 7 cut(s) 286, 389, 496, 602, 608, 675, 691
HpaII CCGG 1 cut(s) 993
HphI GGTGA 2 cut(s) 256, 903
Hpy166II GTNNAC 3 cut(s) 124, 271, 503
Hpy188I TCNGA 5 cut(s) 588, 607, 613, 1128, 1162
Hpy188III TCNNGA 4 cut(s) 378, 482, 683, 993
Hpy8I GTNNAC 3 cut(s) 124, 271, 503
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 1 cut(s) 505
HpyCH4V TGCA 9 cut(s) 463, 536, 562, 729, 809, 826, 917, 941, 1106
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 299, 938
HpyF3I CTNAG 5 cut(s) 24, 209, 288, 317, 1028
HpySE526I ACGT 1 cut(s) 505
Hsp92II CATG 6 cut(s) 185, 485, 514, 560, 774, 1093
HspAI GCGC 4 cut(s) 355, 450, 469, 932
Kpn2I TCCGGA 1 cut(s) 992
KpnI GGTACC 1 cut(s) 1011
Kzo9I GATC 3 cut(s) 443, 820, 1092
LguI GCTCTTC 1 cut(s) 138
LmnI GCTCC 5 cut(s) 56, 153, 433, 646, 831
Lsp1109I GCAGC 3 cut(s) 124, 929, 1118
LweI GCATC 3 cut(s) 545, 998, 1119
MaeI CTAG 1 cut(s) 975
MaeII ACGT 1 cut(s) 505
MaeIII GTNAC 6 cut(s) 155, 410, 475, 700, 909, 1022
MalI GATC 3 cut(s) 445, 822, 1094
MboI GATC 3 cut(s) 443, 820, 1092
MboII GAAGA 7 cut(s) 63, 66, 72, 155, 170, 428, 830
MflI RGATCY 2 cut(s) 443, 820
MhlI GDGCHC 3 cut(s) 53, 150, 836
MluCI AATT 3 cut(s) 128, 456, 1080
MlyI GAGTC 3 cut(s) 280, 398, 700
MmeI TCCRAC 1 cut(s) 360
MnlI CCTC 9 cut(s) 127, 240, 270, 421, 424, 534, 705, 868, 1168
MroI TCCGGA 1 cut(s) 992
MseI TTAA 5 cut(s) 15, 327, 651, 927, 1038
MslI CAYNNNNRTG 3 cut(s) 531, 588, 666
MspCI CTTAAG 1 cut(s) 1037
MspI CCGG 1 cut(s) 993
MspR9I CCNGG 2 cut(s) 117, 1086
Mva1269I GAATGC 2 cut(s) 595, 1072
MvaI CCWGG 2 cut(s) 117, 1086
MvnI CGCG 1 cut(s) 357
MwoI GCNNNNNNNGC 3 cut(s) 94, 299, 938
NdeII GATC 3 cut(s) 443, 820, 1092
NheI GCTAGC 1 cut(s) 974
NlaIII CATG 6 cut(s) 185, 485, 514, 560, 774, 1093
NlaIV GGNNCC 4 cut(s) 52, 753, 833, 1009
NmuCI GTSAC 4 cut(s) 155, 410, 909, 1022
NsbI TGCGCA 1 cut(s) 470
NspV TTCGAA 1 cut(s) 7
PagI TCATGA 1 cut(s) 481
PaqCI CACCTGC 1 cut(s) 922
PciSI GCTCTTC 1 cut(s) 138
PctI GAATGC 2 cut(s) 595, 1072
PfeI GAWTC 4 cut(s) 496, 602, 608, 675
PkrI GCNGC 3 cut(s) 114, 919, 1108
PleI GAGTC 3 cut(s) 280, 397, 699
PpsI GAGTC 3 cut(s) 280, 397, 699
Ppu21I YACGTR 1 cut(s) 506
PpuMI RGGWCCY 1 cut(s) 626
PshAI GACNNNNGTC 1 cut(s) 690
PsiI TTATAA 1 cut(s) 1119
Psp124BI GAGCTC 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 626
Psp6I CCWGG 2 cut(s) 115, 1084
PspFI CCCAGC 1 cut(s) 281
PspGI CCWGG 2 cut(s) 115, 1084
PspN4I GGNNCC 4 cut(s) 52, 753, 833, 1009
PspPI GGNCC 3 cut(s) 86, 626, 1166
PspPPI RGGWCCY 1 cut(s) 626
PstI CTGCAG 3 cut(s) 828, 919, 1108
PstNI CAGNNNCTG 1 cut(s) 845
PsuI RGATCY 2 cut(s) 443, 820
RsaI GTAC 3 cut(s) 225, 272, 1009
RsaNI GTAC 3 cut(s) 224, 271, 1008
RseI CAYNNNNRTG 3 cut(s) 531, 588, 666
SacI GAGCTC 1 cut(s) 150
SapI GCTCTTC 1 cut(s) 138
SaqAI TTAA 5 cut(s) 15, 327, 651, 927, 1038
SatI GCNGC 3 cut(s) 113, 918, 1107
Sau3AI GATC 3 cut(s) 443, 820, 1092
Sau96I GGNCC 3 cut(s) 86, 626, 1166
SchI GAGTC 3 cut(s) 280, 398, 700
ScrFI CCNGG 2 cut(s) 117, 1086
SduI GDGCHC 3 cut(s) 53, 150, 836
SfaNI GCATC 3 cut(s) 545, 998, 1119
SfcI CTRYAG 4 cut(s) 824, 837, 915, 1104
SfuI TTCGAA 1 cut(s) 7
SinI GGWCC 2 cut(s) 626, 1166
SmiMI CAYNNNNRTG 3 cut(s) 531, 588, 666
SmlI CTYRAG 1 cut(s) 1037
SmoI CTYRAG 1 cut(s) 1037
Sse9I AATT 3 cut(s) 128, 456, 1080
SspI AATATT 1 cut(s) 1123
SspMI CTAG 1 cut(s) 975
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 115, 1084
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 1 cut(s) 508
TaqI TCGA 3 cut(s) 7, 392, 684
TasI AATT 3 cut(s) 128, 456, 1080
TatI WGTACW 1 cut(s) 270
TfiI GAWTC 4 cut(s) 496, 602, 608, 675
Tru1I TTAA 5 cut(s) 15, 327, 651, 927, 1038
Tru9I TTAA 5 cut(s) 15, 327, 651, 927, 1038
TscAI CASTG 4 cut(s) 158, 369, 651, 833
TseFI GTSAC 4 cut(s) 155, 410, 909, 1022
TseI GCWGC 3 cut(s) 112, 917, 1106
Tsp45I GTSAC 4 cut(s) 155, 410, 909, 1022
TspDTI ATGAA 4 cut(s) 170, 498, 684, 1055
TspGWI ACGGA 3 cut(s) 780, 856, 1010
TspRI CASTG 4 cut(s) 158, 369, 651, 833
Vha464I CTTAAG 1 cut(s) 1037
VpaK11BI GGWCC 2 cut(s) 626, 1166
XapI RAATTY 1 cut(s) 1080
XcmI CCANNNNNNNNNTGG 1 cut(s) 105
XmiI GTMKAC 1 cut(s) 502
XspI CTAG 1 cut(s) 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.