pycom02g11940

Cupin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012303
Physical Location & Seq
Reverse (-)
8517329 .. 8520259
2931 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1896 bp
ATGTTCAGAAAAGCTTTCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTCTTTCTGTTTCTGTGTCTTTGCATGCAGCAAATGCACTGAAAAATGAAGGGGAAATTGGTTCAGGCGGCGACGGTGCTGCTCCGGCTGGGTCTCTGGTGAAGAAAGATCAACGGCGGTCGCTGGTTTCGACTGAGTACGGAGAGGTCTGGTCCGTTGATGTGAATGACGGACACAGGAAACCCTACCATATTCAGTTCATTACTTTGGAACCCAACTCACTCTTCCTTCCTGTTCTTCTGCATGCATACATGGTTTTCTATGTCAACACAGGGAGTGGGAGGTTGAGTTGGACTGATGATGATAAAATTAGGAGGGTGGATATAAAGAGAGGTGATCTCTATAGGCTCACCCCAGGCTCTGTTTTCTTTGTACAAACAGTAGGGCCATACTCCAGCATTAGGGACCTGGTCCGAGGATTTGATCCGAAATTGCTTCGGTCAGCTTTTAAGGTTTCTGAGGAAGTGATAGAATCAATAGTAAATGGAACGGACCAATCCGCCATTGTCCACGCAATTCCAACAAAGAAAGAAACTTTCTGGGATTTGGAGGCTCGATTCCTGAAAACGTTTCTACTAGGCAATGATGGCATTGCATTCAACAGTAAGAAGAAGAAGGAAACAACAACATATAATATTTTTGACGAGGATCCGGATTTCGAGAATTGCAATGGTTGGAGCCTAACTGTGAGCAAGAAAAACACCCACTTGTTAAAGGGATCAAACATTGGTTTTTTCATGGTGAATTTGACAAAGGGGTCAATGATGGGGCCTCATTGGAATCCAAGGGCAACTGAGATAACAGTAGTACTACAAGGGCACGGGATGGTTAGAGTGGTTTGTTCAAGCACCGAAGCAAAGAAATCGGAGTGCAGAAACATGCGTTTTAGGGTTCATGAAGGAGATGTATTTTCTGTGCCTAGGTTCCATCCAATGGCTCAGATGTCATTTAACAATGACTCTCTTGTGTTCATGGGGTTTAGCACAACAGAAAAGAAAAACTATCCTCAGTTTTTGGCCGGAAAATACTCGGTTCTGCAGACTTTGGACAAACTAGTCTTGGCGGCGTCGTTTAATGTTACCAGCACAACCATTGATCAGCTTTTGTCTGCACAGGCTGACTCATTTATAATAGATTGTACTTCTTGTGCTGAAGAAGAGGAGCAAGCGATGTTGGAAGAGATTGAAAAGAAAAGGGAGGAAGAGGAAGCTAAGAAGAGGGAGAAAGAGGAGGCTCGAAAGAGAGAGCAGGAGGAACAAGACAGAAAGAGGGAGGAGGAGGAAGCTAAGAAGAGGGAGGAAGAGGAAAGGGAGAAGGAAGCAAGGAAACAAGAAGAAGAGCAAGCGGCGAGGGAGCGAGAGGAGGAAGCGGTGAGGGAGAGAGAGAAGGAAGCCGCAAGGGAACAAGAAGAGGAAGCCGCAAGAGAGAGAGAGGAGGAAGCCGCAAGGGAACAAGAAGAGCAAGCAGCAAGGGAGAGAGAGGAGCAAGCCGCAAAACAGCTAGAAGAGCAAGCAGCAAGGGAGAGAGAAGAGCAAGAAGCGGCAAGGGAGCGAGAAGAACAAGAAGCTGCAAGGGAGCGAGAAGAAGAAGCAGCAAGAAGGAGAGAAGAGGAGGGCAGGCAAGGAGGCGATGAAAGAAGACGAGAGGAAGAAAGAGAGAGGGAGAGGAGGCAACAAGAGGAATGGGAAAGGCGAGAGGAAGATGCGCAGAGGGAGCAGGAAGAGGCTCGAAGACAACAACAAGAAAGGCAAGGCAGAGCAGGACAACCAGAGGAGCAAGAGGGTGGAGGCGGAGGCTTCAATGCGGATGAAGGAAGGAGGTATCTGAGTGCATTGAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

632

Amino Acids

73.24

Weight (kDa)

5.15

Isoelectric Point (pI)

65.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 228 - 382 7.8e-38 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36700
fragaria_vesca FvH4_1g13730 FvH4_1g13730 FvH4_1g13730
malus_domestica MD02G1152000.v1.1 MD15G1266400.v1.1
prunus_persica Prupe.7G151000_v2.0.a1 Prupe.7G151000_v2.0.a1
pyrus_communis pycom02g11940
rosa_chinensis RchiOBHm_Chr2g0102141
rosa_laevigata RLG00000017133
rosa_roxburghii Rroxscaffold_2G00140750
rosa_rugosa Rorug02G0104600 Rorug02G0104700
rosa_samantha Rh2AG153100 Rh2BG159000 Rh2CG158300 Rh2DG158700
rosa_wichuraiana Rw2G011950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1181
AasI GACNNNNNNGTC 2 cut(s) 808, 1106
Acc16I TGCGCA 1 cut(s) 1758
AccB7I CCANNNNNTGG 1 cut(s) 985
AccIII TCCGGA 1 cut(s) 703
AclI AACGTT 1 cut(s) 620
AclWI GGATC 4 cut(s) 470, 695, 708, 778
AcoI YGGCCR 1 cut(s) 1068
AcsI RAATTY 1 cut(s) 796
AcuI CTGAAG 1 cut(s) 1224
AcyI GRCGYC 1 cut(s) 1118
AfaI GTAC 4 cut(s) 191, 426, 861, 1192
AfiI CCNNNNNNNGG 2 cut(s) 453, 985
AgsI TTSAA 5 cut(s) 652, 897, 1238, 1852, 1888
AhlI ACTAGT 1 cut(s) 1105
AjnI CCWGG 2 cut(s) 406, 459
AjuI GAANNNNNNNTTGG 4 cut(s) 93, 125, 260, 292
AluBI AGCT 7 cut(s) 14, 497, 1153, 1262, 1337, 1552, 1619
AluI AGCT 7 cut(s) 14, 497, 1153, 1262, 1337, 1552, 1619
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 4 cut(s) 470, 695, 708, 778
AlwNI CAGNNNCTG 1 cut(s) 413
Aor13HI TCCGGA 1 cut(s) 703
AoxI GGCC 3 cut(s) 437, 821, 1068
ApeKI GCWGC 6 cut(s) 80, 131, 1517, 1565, 1619, 1643
ApoI RAATTY 1 cut(s) 796
AspA2I CCTAGG 1 cut(s) 971
AspLEI GCGC 1 cut(s) 1759
AspS9I GGNCC 6 cut(s) 204, 437, 457, 463, 544, 821
AsuHPI GGTGA 5 cut(s) 163, 394, 398, 805, 1435
AvaII GGWCC 4 cut(s) 204, 457, 463, 544
AvrII CCTAGG 1 cut(s) 971
BaeGI GKGCMC 1 cut(s) 873
BamHI GGATCC 1 cut(s) 700
BbsI GAAGAC 2 cut(s) 1696, 1789
BbvI GCAGC 6 cut(s) 92, 118, 1529, 1577, 1606, 1655
BccI CCATC 4 cut(s) 632, 811, 871, 987
BceAI ACGGC 1 cut(s) 182
BcgI CGANNNNNNTGC 4 cut(s) 113, 147, 897, 931
BciT130I CCWGG 2 cut(s) 408, 461
BclI TGATCA 1 cut(s) 1147
BcoDI GTCTC 1 cut(s) 150
BcuI ACTAGT 1 cut(s) 1105
BfaI CTAG 4 cut(s) 629, 972, 1106, 1553
BfmI CTRYAG 2 cut(s) 394, 1088
BlnI CCTAGG 1 cut(s) 971
BmcAI AGTACT 1 cut(s) 861
Bme1390I CCNGG 2 cut(s) 408, 461
Bme18I GGWCC 4 cut(s) 204, 457, 463, 544
BmgT120I GGNCC 6 cut(s) 204, 437, 457, 463, 544, 821
BmiI GGNNCC 6 cut(s) 264, 458, 702, 731, 822, 977
BmrFI CCNGG 2 cut(s) 408, 461
BmsI GCATC 1 cut(s) 1744
BpiI GAAGAC 2 cut(s) 1696, 1789
BplI GAGNNNNNCTC 2 cut(s) 375, 407
BpmI CTGGAG 1 cut(s) 430
BsaHI GRCGYC 1 cut(s) 1118
BsaI GGTCTC 1 cut(s) 150
BsaJI CCNNGG 4 cut(s) 406, 466, 836, 971
BsaWI WCCGGW 1 cut(s) 703
BsaXI ACNNNNNCTCC 2 cut(s) 325, 355
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 985
Bse3DI GCAATG 3 cut(s) 640, 642, 727
BseAI TCCGGA 1 cut(s) 703
BseBI CCWGG 2 cut(s) 408, 461
BseDI CCNNGG 4 cut(s) 406, 466, 836, 971
BseGI GGATG 3 cut(s) 882, 979, 1864
BseLI CCNNNNNNNGG 2 cut(s) 453, 985
BseMI GCAATG 3 cut(s) 640, 642, 727
BseMII CTCAG 6 cut(s) 177, 501, 837, 1004, 1073, 1868
BseSI GKGCMC 1 cut(s) 873
BseXI GCAGC 6 cut(s) 92, 118, 1529, 1577, 1606, 1655
BseYI CCCAGC 1 cut(s) 140
BsgI GTGCAG 2 cut(s) 943, 1146
Bsh1285I CGRYCG 1 cut(s) 173
BshFI GGCC 3 cut(s) 439, 823, 1070
BsiEI CGRYCG 1 cut(s) 173
BsiSI CCGG 3 cut(s) 137, 704, 1071
BslFI GGGAC 1 cut(s) 470
BslI CCNNNNNNNGG 2 cut(s) 453, 985
BsmAI GTCTC 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 470
BsmI GAATGC 1 cut(s) 647
BsnI GGCC 3 cut(s) 439, 823, 1070
Bso31I GGTCTC 1 cut(s) 150
Bsp1286I GDGCHC 1 cut(s) 873
Bsp13I TCCGGA 1 cut(s) 703
Bsp1407I TGTACA 1 cut(s) 424
Bsp143I GATC 6 cut(s) 160, 388, 475, 700, 770, 1147
BspANI GGCC 3 cut(s) 439, 823, 1070
BspCNI CTCAG 6 cut(s) 178, 502, 838, 1003, 1072, 1869
BspEI TCCGGA 1 cut(s) 703
BspHI TCATGA 1 cut(s) 946
BspLI GGNNCC 6 cut(s) 264, 458, 702, 731, 822, 977
BspMAI CTGCAG 1 cut(s) 1092
BspPI GGATC 4 cut(s) 470, 695, 708, 778
BspQI GCTCTTC 4 cut(s) 1383, 1503, 1551, 1575
BspTNI GGTCTC 1 cut(s) 150
BsrDI GCAATG 3 cut(s) 640, 642, 727
BsrGI TGTACA 1 cut(s) 424
BssECI CCNNGG 4 cut(s) 406, 466, 836, 971
BssMI GATC 6 cut(s) 160, 388, 475, 700, 770, 1147
BssNI GRCGYC 1 cut(s) 1118
BssT1I CCWWGG 2 cut(s) 836, 971
Bst2UI CCWGG 2 cut(s) 408, 461
Bst4CI ACNGT 5 cut(s) 128, 433, 656, 739, 856
BstACI GRCGYC 1 cut(s) 1118
BstAPI GCANNNNNTGC 1 cut(s) 86
BstAUI TGTACA 1 cut(s) 424
BstC8I GCNNGC 8 cut(s) 78, 297, 1218, 1395, 1515, 1539, 1563, 1670
BstDEI CTNAG 8 cut(s) 186, 510, 846, 990, 1059, 1263, 1338, 1877
BstF5I GGATG 3 cut(s) 882, 979, 1864
BstHHI GCGC 1 cut(s) 1759
BstKTI GATC 6 cut(s) 163, 391, 478, 703, 773, 1150
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 6 cut(s) 160, 388, 475, 700, 770, 1147
BstMCI CGRYCG 1 cut(s) 173
BstMWI GCNNNNNNNGC 5 cut(s) 86, 137, 639, 1558, 1765
BstNI CCWGG 2 cut(s) 408, 461
BstNSI RCATGY 3 cut(s) 80, 299, 934
BstSCI CCNGG 2 cut(s) 406, 459
BstSFI CTRYAG 2 cut(s) 394, 1088
BstSLI GKGCMC 1 cut(s) 873
BstV1I GCAGC 6 cut(s) 92, 118, 1529, 1577, 1606, 1655
BstV2I GAAGAC 2 cut(s) 1696, 1789
BstX2I RGATCY 1 cut(s) 700
BstYI RGATCY 1 cut(s) 700
BsuRI GGCC 3 cut(s) 439, 823, 1070
BtgZI GCGATG 2 cut(s) 1235, 1695
BtsCI GGATG 3 cut(s) 882, 979, 1864
BtsIMutI CAGTG 1 cut(s) 89
Cac8I GCNNGC 8 cut(s) 78, 297, 1218, 1395, 1515, 1539, 1563, 1670
CaiI CAGNNNCTG 1 cut(s) 413
CciI TCATGA 1 cut(s) 946
CfoI GCGC 1 cut(s) 1759
Cfr13I GGNCC 6 cut(s) 204, 437, 457, 463, 544, 821
CseI GACGC 1 cut(s) 1107
CsiI ACCWGGT 1 cut(s) 459
Csp6I GTAC 4 cut(s) 190, 425, 860, 1191
CviAII CATG 7 cut(s) 77, 296, 304, 790, 931, 947, 1024
CviQI GTAC 4 cut(s) 190, 425, 860, 1191
DdeI CTNAG 8 cut(s) 186, 510, 846, 990, 1059, 1263, 1338, 1877
DpnI GATC 6 cut(s) 162, 390, 477, 702, 772, 1149
DpnII GATC 6 cut(s) 160, 388, 475, 700, 770, 1147
DrdI GACNNNNNNGTC 2 cut(s) 808, 1106
DseDI GACNNNNNNGTC 2 cut(s) 808, 1106
EaeI YGGCCR 1 cut(s) 1068
EciI GGCGGA 2 cut(s) 541, 1857
Eco130I CCWWGG 2 cut(s) 836, 971
Eco31I GGTCTC 1 cut(s) 150
Eco47I GGWCC 4 cut(s) 204, 457, 463, 544
Eco57I CTGAAG 1 cut(s) 1224
EcoO109I RGGNCCY 2 cut(s) 457, 821
EcoRII CCWGG 2 cut(s) 406, 459
EcoT14I CCWWGG 2 cut(s) 836, 971
EcoT22I ATGCAT 1 cut(s) 301
ErhI CCWWGG 2 cut(s) 836, 971
FaeI CATG 7 cut(s) 80, 299, 307, 793, 934, 950, 1027
FaqI GGGAC 1 cut(s) 470
FatI CATG 7 cut(s) 76, 295, 303, 789, 930, 946, 1023
FbaI TGATCA 1 cut(s) 1147
FokI GGATG 3 cut(s) 889, 966, 1871
FspBI CTAG 4 cut(s) 629, 972, 1106, 1553
FspI TGCGCA 1 cut(s) 1758
GlaI GCGC 1 cut(s) 1758
GsaI CCCAGC 1 cut(s) 144
GsuI CTGGAG 1 cut(s) 430
HaeIII GGCC 3 cut(s) 439, 823, 1070
HapII CCGG 3 cut(s) 137, 704, 1071
HgaI GACGC 1 cut(s) 1107
HhaI GCGC 1 cut(s) 1759
Hin1I GRCGYC 1 cut(s) 1118
Hin1II CATG 7 cut(s) 80, 299, 307, 793, 934, 950, 1027
Hin6I GCGC 1 cut(s) 1757
HinP1I GCGC 1 cut(s) 1757
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 5 cut(s) 524, 609, 832, 1010, 1172
HpaII CCGG 3 cut(s) 137, 704, 1071
HphI GGTGA 5 cut(s) 163, 394, 398, 805, 1435
Hpy166II GTNNAC 2 cut(s) 319, 562
Hpy188I TCNGA 7 cut(s) 8, 467, 480, 511, 919, 993, 1878
Hpy188III TCNNGA 4 cut(s) 613, 704, 712, 947
Hpy8I GTNNAC 2 cut(s) 319, 562
Hpy99I CGWCG 2 cut(s) 128, 1123
HpyCH4III ACNGT 5 cut(s) 128, 433, 656, 739, 856
HpyCH4IV ACGT 1 cut(s) 620
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 137, 639, 1558, 1765
HpyF3I CTNAG 8 cut(s) 186, 510, 846, 990, 1059, 1263, 1338, 1877
HpySE526I ACGT 1 cut(s) 620
Hsp92I GRCGYC 1 cut(s) 1118
Hsp92II CATG 7 cut(s) 80, 299, 307, 793, 934, 950, 1027
HspAI GCGC 1 cut(s) 1757
Kpn2I TCCGGA 1 cut(s) 703
Ksp22I TGATCA 1 cut(s) 1147
Kzo9I GATC 6 cut(s) 160, 388, 475, 700, 770, 1147
LguI GCTCTTC 4 cut(s) 1383, 1503, 1551, 1575
LmnI GCTCC 9 cut(s) 139, 729, 1213, 1405, 1534, 1600, 1627, 1765, 1825
Lsp1109I GCAGC 6 cut(s) 92, 118, 1529, 1577, 1606, 1655
LweI GCATC 1 cut(s) 1744
MabI ACCWGGT 1 cut(s) 459
MaeI CTAG 4 cut(s) 629, 972, 1106, 1553
MaeII ACGT 1 cut(s) 620
MaeIII GTNAC 1 cut(s) 1129
MalI GATC 6 cut(s) 162, 390, 477, 702, 772, 1149
MboI GATC 6 cut(s) 160, 388, 475, 700, 770, 1147
MflI RGATCY 1 cut(s) 700
MhlI GDGCHC 1 cut(s) 873
MluCI AATT 6 cut(s) 108, 360, 482, 567, 715, 796
MlyI GAGTC 2 cut(s) 1004, 1166
MmeI TCCRAC 4 cut(s) 323, 596, 707, 1206
Mph1103I ATGCAT 1 cut(s) 301
MroI TCCGGA 1 cut(s) 703
MseI TTAA 5 cut(s) 501, 764, 1002, 1125, 1894
MspI CCGG 3 cut(s) 137, 704, 1071
MspR9I CCNGG 2 cut(s) 408, 461
Mva1269I GAATGC 1 cut(s) 647
MvaI CCWGG 2 cut(s) 408, 461
MwoI GCNNNNNNNGC 5 cut(s) 86, 137, 639, 1558, 1765
NdeII GATC 6 cut(s) 160, 388, 475, 700, 770, 1147
NlaIII CATG 7 cut(s) 80, 299, 307, 793, 934, 950, 1027
NlaIV GGNNCC 6 cut(s) 264, 458, 702, 731, 822, 977
NsbI TGCGCA 1 cut(s) 1758
NsiI ATGCAT 1 cut(s) 301
NspI RCATGY 3 cut(s) 80, 299, 934
PaeI GCATGC 2 cut(s) 80, 299
PagI TCATGA 1 cut(s) 946
PciSI GCTCTTC 4 cut(s) 1383, 1503, 1551, 1575
PcsI WCGNNNNNNNCGW 1 cut(s) 179
PctI GAATGC 1 cut(s) 647
PfeI GAWTC 3 cut(s) 524, 609, 832
PflFI GACNNNGTC 1 cut(s) 461
PflMI CCANNNNNTGG 1 cut(s) 985
PleI GAGTC 2 cut(s) 1004, 1166
PpsI GAGTC 2 cut(s) 1004, 1166
PpuMI RGGWCCY 1 cut(s) 457
PsiI TTATAA 1 cut(s) 1181
Psp1406I AACGTT 1 cut(s) 620
Psp5II RGGWCCY 1 cut(s) 457
Psp6I CCWGG 2 cut(s) 406, 459
PspFI CCCAGC 1 cut(s) 140
PspGI CCWGG 2 cut(s) 406, 459
PspN4I GGNNCC 6 cut(s) 264, 458, 702, 731, 822, 977
PspPI GGNCC 6 cut(s) 204, 437, 457, 463, 544, 821
PspPPI RGGWCCY 1 cut(s) 457
PstI CTGCAG 1 cut(s) 1092
PstNI CAGNNNCTG 1 cut(s) 413
PsuI RGATCY 1 cut(s) 700
PsyI GACNNNGTC 1 cut(s) 461
RsaI GTAC 4 cut(s) 191, 426, 861, 1192
RsaNI GTAC 4 cut(s) 190, 425, 860, 1191
SapI GCTCTTC 4 cut(s) 1383, 1503, 1551, 1575
SaqAI TTAA 5 cut(s) 501, 764, 1002, 1125, 1894
Sau3AI GATC 6 cut(s) 160, 388, 475, 700, 770, 1147
Sau96I GGNCC 6 cut(s) 204, 437, 457, 463, 544, 821
ScaI AGTACT 1 cut(s) 861
SchI GAGTC 2 cut(s) 1004, 1166
ScrFI CCNGG 2 cut(s) 408, 461
SduI GDGCHC 1 cut(s) 873
SexAI ACCWGGT 1 cut(s) 459
SfaNI GCATC 1 cut(s) 1744
SfcI CTRYAG 2 cut(s) 394, 1088
SinI GGWCC 4 cut(s) 204, 457, 463, 544
SpeI ACTAGT 1 cut(s) 1105
SphI GCATGC 2 cut(s) 80, 299
Sse9I AATT 6 cut(s) 108, 360, 482, 567, 715, 796
SspI AATATT 1 cut(s) 688
SspMI CTAG 4 cut(s) 629, 972, 1106, 1553
StyD4I CCNGG 2 cut(s) 406, 459
StyI CCWWGG 2 cut(s) 836, 971
TaaI ACNGT 5 cut(s) 128, 433, 656, 739, 856
TaiI ACGT 1 cut(s) 623
TaqI TCGA 5 cut(s) 182, 607, 711, 1288, 1780
TaqII GACCGA 1 cut(s) 480
TasI AATT 6 cut(s) 108, 360, 482, 567, 715, 796
TatI WGTACW 3 cut(s) 424, 859, 1190
TauI GCSGC 8 cut(s) 123, 1118, 1400, 1448, 1472, 1496, 1544, 1595
TfiI GAWTC 3 cut(s) 524, 609, 832
Tru1I TTAA 5 cut(s) 501, 764, 1002, 1125, 1894
Tru9I TTAA 5 cut(s) 501, 764, 1002, 1125, 1894
TscAI CASTG 1 cut(s) 96
TseI GCWGC 6 cut(s) 80, 131, 1517, 1565, 1619, 1643
TspDTI ATGAA 9 cut(s) 7, 114, 241, 778, 935, 963, 1012, 1698, 1875
TspGWI ACGGA 4 cut(s) 196, 207, 237, 557
TspRI CASTG 1 cut(s) 96
Tth111I GACNNNGTC 1 cut(s) 461
Van91I CCANNNNNTGG 1 cut(s) 985
VpaK11BI GGWCC 4 cut(s) 204, 457, 463, 544
XapI RAATTY 1 cut(s) 796
XceI RCATGY 3 cut(s) 80, 299, 934
XmaJI CCTAGG 1 cut(s) 971
XspI CTAG 4 cut(s) 629, 972, 1106, 1553
ZrmI AGTACT 1 cut(s) 861
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.