pycom02g21710

Germin-like protein subfamily 3 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0016937
Physical Location & Seq
Reverse (-)
19932675 .. 19933595
921 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g21710.2

Sequence Viewer

Length: 666 bp
ATGTTGGTGGATGCAGTCATTCCCTGGGTCTACAAAACGGTAGCCCTTTTCGTTGTCCTCCTCTTCAACAAGAGCTATGTCACTTTCGCTTCAGATCTGGATCCTGTTCAAGACTTCTGCATAGCCAACACAGAGCCTGCGAGTCATGCCAACCAATGCAAGAACTCATCCCTAGCAACAGTAGAAGATTTTGAATTTTCGGGCATTAGGGTTCCTGGAAAGTTTGGTCCAAGCGGGCTCTCTGGCGTTTCTGTGAACTCAAACGTCTTTCCAGGACTCAACACGCTTGGCATGTCGTTTGTCCGAGCTGACTTTGAAGTTGGTGGAGTAAACGTGCCACATTTTCACCCGAGGGCAATAGAGATTGCATTTGTGCTCGAAGGAAAGATCTATTCGGGGTTTGTTGATACGACTAACAGGATTTTGGCCAAAGTGAACGAAAAAGGTGAGGTGATGGTGTTTCCAAGGAGTCTAGTGCACTTTCAAATGAATGTTGGTGATACTCCGGCAACTAAATTGGGCAGTTTTGACAGCCAAAACCCTGGAACCCAGAAAATCCCCGCTGCAATTTTCGGATCCGGTATCGAAGATAAACTCTTGGAAAAAGCTTTTGGATTGAGTTCGAAGGAGATTTCAAAGTTGAAAAAGAAGCTTGGTTCACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

222

Amino Acids

23.9

Weight (kDa)

6.96

Isoelectric Point (pI)

22.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016937)

Species Orthologous Gene IDs
malus_domestica MD02G1256600.v1.1 MD07G1063800.v1.1
prunus_persica Prupe.2G077100_v2.0.a1
pyrus_communis pycom02g21710 pycom07g04920
rosa_chinensis RchiOBHm_Chr1g0332401
rosa_laevigata RLG00000029673
rosa_multiflora Rmu_ssc0000161.1_g000016
rosa_roxburghii Rroxscaffold_4G00318210
rosa_rugosa Rorug01G0100500.1
rosa_samantha Rh1AG124400 Rh1CG118600 Rh1DG129500
rosa_wichuraiana Rw1G010120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 30
AciI CCGC 2 cut(s) 234, 561
AclWI GGATC 4 cut(s) 95, 108, 570, 583
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 1 cut(s) 75
AgsI TTSAA 7 cut(s) 67, 110, 194, 317, 485, 636, 643
AjnI CCWGG 4 cut(s) 23, 214, 271, 541
AjuI GAANNNNNNNTTGG 2 cut(s) 594, 626
AluBI AGCT 4 cut(s) 75, 308, 608, 652
AluI AGCT 4 cut(s) 75, 308, 608, 652
Alw21I GWGCWC 2 cut(s) 378, 480
Alw44I GTGCAC 1 cut(s) 476
AlwI GGATC 4 cut(s) 95, 108, 570, 583
AlwNI CAGNNNCTG 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 349
AoxI GGCC 1 cut(s) 426
ApaLI GTGCAC 1 cut(s) 476
ApeKI GCWGC 1 cut(s) 563
ApoI RAATTY 1 cut(s) 194
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 4 cut(s) 338, 458, 463, 509
AsuII TTCGAA 1 cut(s) 623
AvaI CYCGRG 1 cut(s) 349
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 1 cut(s) 480
BalI TGGCCA 1 cut(s) 428
BamHI GGATCC 2 cut(s) 100, 575
BanII GRGCYC 1 cut(s) 240
Bbv12I GWGCWC 2 cut(s) 378, 480
BbvI GCAGC 1 cut(s) 550
BccI CCATC 1 cut(s) 448
BciT130I CCWGG 4 cut(s) 25, 216, 273, 543
BfaI CTAG 2 cut(s) 173, 473
BglII AGATCT 2 cut(s) 94, 387
BisI GCNGC 1 cut(s) 564
BlsI GCNGC 1 cut(s) 565
Bme1390I CCNGG 4 cut(s) 25, 216, 273, 543
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 349
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 4 cut(s) 102, 213, 547, 577
BmrFI CCNGG 4 cut(s) 25, 216, 273, 543
Bpu14I TTCGAA 1 cut(s) 623
BsaBI GATNNNNATC 1 cut(s) 99
BsaJI CCNNGG 5 cut(s) 23, 24, 350, 464, 541
BsaWI WCCGGW 1 cut(s) 578
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bse8I GATNNNNATC 1 cut(s) 99
BseBI CCWGG 4 cut(s) 25, 216, 273, 543
BseDI CCNNGG 5 cut(s) 23, 24, 350, 464, 541
BseGI GGATG 2 cut(s) 16, 167
BseJI GATNNNNATC 1 cut(s) 99
BseRI GAGGAG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 1 cut(s) 550
BshFI GGCC 1 cut(s) 428
BsiHKAI GWGCWC 2 cut(s) 378, 480
BsiHKCI CYCGRG 1 cut(s) 349
BsiSI CCGG 2 cut(s) 506, 579
BsnI GGCC 1 cut(s) 428
BsoBI CYCGRG 1 cut(s) 349
Bsp119I TTCGAA 1 cut(s) 623
Bsp1286I GDGCHC 3 cut(s) 240, 378, 480
Bsp143I GATC 4 cut(s) 94, 100, 387, 575
BspACI CCGC 2 cut(s) 234, 561
BspANI GGCC 1 cut(s) 428
BspLI GGNNCC 4 cut(s) 102, 213, 547, 577
BspPI GGATC 4 cut(s) 95, 108, 570, 583
BspT104I TTCGAA 1 cut(s) 623
BssECI CCNNGG 5 cut(s) 23, 24, 350, 464, 541
BssMI GATC 4 cut(s) 94, 100, 387, 575
BssT1I CCWWGG 1 cut(s) 464
Bst2UI CCWGG 4 cut(s) 25, 216, 273, 543
Bst4CI ACNGT 2 cut(s) 40, 181
Bst6I CTCTTC 1 cut(s) 68
BstBI TTCGAA 1 cut(s) 623
BstC8I GCNNGC 2 cut(s) 138, 236
BstF5I GGATG 2 cut(s) 16, 167
BstKTI GATC 4 cut(s) 97, 103, 390, 578
BstMBI GATC 4 cut(s) 94, 100, 387, 575
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 4 cut(s) 25, 216, 273, 543
BstNSI RCATGY 1 cut(s) 295
BstSCI CCNGG 4 cut(s) 23, 214, 271, 541
BstSLI GKGCMC 1 cut(s) 480
BstV1I GCAGC 1 cut(s) 550
BstX2I RGATCY 4 cut(s) 94, 100, 387, 575
BstXI CCANNNNNNTGG 1 cut(s) 542
BstYI RGATCY 4 cut(s) 94, 100, 387, 575
BsuRI GGCC 1 cut(s) 428
BtsCI GGATG 2 cut(s) 16, 167
Cac8I GCNNGC 2 cut(s) 138, 236
CaiI CAGNNNCTG 1 cut(s) 137
Cfr13I GGNCC 1 cut(s) 227
CviAII CATG 2 cut(s) 146, 292
DpnI GATC 4 cut(s) 96, 102, 389, 577
DpnII GATC 4 cut(s) 94, 100, 387, 575
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
Eco130I CCWWGG 1 cut(s) 464
Eco24I GRGCYC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 349
EcoRII CCWGG 4 cut(s) 23, 214, 271, 541
EcoT14I CCWWGG 1 cut(s) 464
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 464
FaeI CATG 2 cut(s) 149, 295
FaiI YATR 4 cut(s) 78, 122, 147, 293
FatI CATG 2 cut(s) 145, 291
FauI CCCGC 2 cut(s) 227, 568
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 1 cut(s) 564
FokI GGATG 2 cut(s) 23, 154
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 564
FspBI CTAG 2 cut(s) 173, 473
GluI GCNGC 1 cut(s) 564
HaeIII GGCC 1 cut(s) 428
HapII CCGG 2 cut(s) 506, 579
Hin1II CATG 2 cut(s) 149, 295
HindIII AAGCTT 2 cut(s) 606, 650
HinfI GANTC 3 cut(s) 142, 276, 469
HpaII CCGG 2 cut(s) 506, 579
HphI GGTGA 4 cut(s) 338, 458, 463, 509
Hpy166II GTNNAC 6 cut(s) 31, 256, 331, 436, 478, 659
Hpy188I TCNGA 3 cut(s) 94, 305, 575
Hpy188III TCNNGA 2 cut(s) 98, 110
Hpy8I GTNNAC 6 cut(s) 31, 256, 331, 436, 478, 659
HpyAV CCTTC 2 cut(s) 374, 619
HpyCH4III ACNGT 2 cut(s) 40, 181
HpyCH4IV ACGT 2 cut(s) 264, 333
HpyCH4V TGCA 6 cut(s) 14, 120, 159, 368, 478, 566
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpySE526I ACGT 2 cut(s) 264, 333
Hsp92II CATG 2 cut(s) 149, 295
Kzo9I GATC 4 cut(s) 94, 100, 387, 575
Lsp1109I GCAGC 1 cut(s) 550
MaeI CTAG 2 cut(s) 173, 473
MaeII ACGT 2 cut(s) 264, 333
MaeIII GTNAC 1 cut(s) 79
MalI GATC 4 cut(s) 96, 102, 389, 577
MboI GATC 4 cut(s) 94, 100, 387, 575
MboII GAAGA 3 cut(s) 55, 197, 599
MflI RGATCY 4 cut(s) 94, 100, 387, 575
MhlI GDGCHC 3 cut(s) 240, 378, 480
MlsI TGGCCA 1 cut(s) 428
MluCI AATT 3 cut(s) 194, 515, 567
MluNI TGGCCA 1 cut(s) 428
MlyI GAGTC 3 cut(s) 151, 270, 478
MnlI CCTC 4 cut(s) 68, 71, 345, 442
Mox20I TGGCCA 1 cut(s) 428
MscI TGGCCA 1 cut(s) 428
Msp20I TGGCCA 1 cut(s) 428
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 2 cut(s) 506, 579
MspR9I CCNGG 4 cut(s) 25, 216, 273, 543
MvaI CCWGG 4 cut(s) 25, 216, 273, 543
MwoI GCNNNNNNNGC 1 cut(s) 146
NdeII GATC 4 cut(s) 94, 100, 387, 575
NlaIII CATG 2 cut(s) 149, 295
NlaIV GGNNCC 4 cut(s) 102, 213, 547, 577
NmuCI GTSAC 1 cut(s) 79
NspI RCATGY 1 cut(s) 295
NspV TTCGAA 1 cut(s) 623
PasI CCCWGGG 1 cut(s) 24
PfoI TCCNGGA 2 cut(s) 214, 271
PkrI GCNGC 1 cut(s) 565
PleI GAGTC 3 cut(s) 150, 270, 477
PpsI GAGTC 3 cut(s) 150, 270, 477
Psp6I CCWGG 4 cut(s) 23, 214, 271, 541
PspGI CCWGG 4 cut(s) 23, 214, 271, 541
PspN4I GGNNCC 4 cut(s) 102, 213, 547, 577
PspPI GGNCC 1 cut(s) 227
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 4 cut(s) 94, 100, 387, 575
SatI GCNGC 1 cut(s) 564
Sau3AI GATC 4 cut(s) 94, 100, 387, 575
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 3 cut(s) 151, 270, 478
ScrFI CCNGG 4 cut(s) 25, 216, 273, 543
SduI GDGCHC 3 cut(s) 240, 378, 480
SetI ASST 8 cut(s) 77, 267, 310, 336, 448, 453, 610, 654
SfuI TTCGAA 1 cut(s) 623
SinI GGWCC 1 cut(s) 227
Sse9I AATT 3 cut(s) 194, 515, 567
SsiI CCGC 2 cut(s) 234, 561
SspMI CTAG 2 cut(s) 173, 473
StyD4I CCNGG 4 cut(s) 23, 214, 271, 541
StyI CCWWGG 1 cut(s) 464
TaaI ACNGT 2 cut(s) 40, 181
TaiI ACGT 2 cut(s) 267, 336
TaqI TCGA 3 cut(s) 378, 585, 623
TasI AATT 3 cut(s) 194, 515, 567
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 1 cut(s) 563
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 1 cut(s) 503
VneI GTGCAC 1 cut(s) 476
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 1 cut(s) 194
XceI RCATGY 1 cut(s) 295
XmiI GTMKAC 1 cut(s) 30
XspI CTAG 2 cut(s) 173, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.