Rw1G010120

Germin-like protein subfamily 3 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016937
Physical Location & Seq
Forward (+)
22417575 .. 22418572
998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G010120.1

Sequence Viewer

Length: 645 bp
ATGTTCACCAAAATGGTACTTCTTTTTGTGATCTTCCTCAACATCTATGTCAGTTTGGCTTCTGATCCGGATCCGGTCCAAGACTTCTGCATAGCCAACACAGCAGAATCTGCAACTGCAAGCAATGCCATCCAATGCAAGAATTCATCCCTTGCAACAGTAGAAGATTTTGTATATTCTGGCATCAAGTCTCCTGGAAAATTTGGCCAAACAGGTCTTTCTGCCATTTCAGTGAACTCAAATGTCTTTCCAGGACTGAACACACTTGGAATGTCATTTGTCCGAGCTGATTTTGAAGTTGGTGGTGTAAATGTGCCTCATTACCATCCGAGGGCAACGGAGACAGCCTTTGTGCTTGAAGGAAAGATTTACTCCGGATTTGTTGATACGAATAACAAGATTTTCGCTAAAGTGATCGAAAGGGGTGAGGTCATGGTGTTTCCAAAAGGGTTAGTGCACTTCCAAATGAATGTTGGTGACACTCCAGCAACCATATTGGGGAGCTTCAACAGCCAAAATCCTGGATTGCAAAGAATTCCCACTTCAATTTTCGGATCCGGGATCAAAGATGAGCTCTTGGAGAAGGCTTTTGGATTGAGTTCTAAGGAGATTGCCAAGTTGAAAAAGAAGCTTGGTCCCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

214

Amino Acids

23.07

Weight (kDa)

7.76

Isoelectric Point (pI)

23.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 62 - 204 1.1e-42 Cupin
Cupin_2 PF07883 96 - 166 8.2e-10 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016937)

Species Orthologous Gene IDs
malus_domestica MD02G1256600.v1.1 MD07G1063800.v1.1
prunus_persica Prupe.2G077100_v2.0.a1
pyrus_communis pycom02g21710 pycom07g04920
rosa_chinensis RchiOBHm_Chr1g0332401
rosa_laevigata RLG00000029673
rosa_multiflora Rmu_ssc0000161.1_g000016
rosa_roxburghii Rroxscaffold_4G00318210
rosa_rugosa Rorug01G0100500.1
rosa_samantha Rh1AG124400 Rh1CG118600 Rh1DG129500
rosa_wichuraiana Rw1G010120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 67, 374
AclWI GGATC 6 cut(s) 59, 65, 78, 549, 562, 569
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 3 cut(s) 142, 200, 534
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 2 cut(s) 331, 498
AgsI TTSAA 5 cut(s) 296, 359, 508, 546, 622
AjnI CCWGG 3 cut(s) 193, 250, 520
AluBI AGCT 4 cut(s) 287, 504, 574, 631
AluI AGCT 4 cut(s) 287, 504, 574, 631
Alw21I GWGCWC 2 cut(s) 459, 576
Alw26I GTCTC 2 cut(s) 195, 335
Alw44I GTGCAC 1 cut(s) 455
AlwI GGATC 6 cut(s) 59, 65, 78, 549, 562, 569
AlwNI CAGNNNCTG 1 cut(s) 110
Aor13HI TCCGGA 2 cut(s) 67, 374
AoxI GGCC 1 cut(s) 205
ApaLI GTGCAC 1 cut(s) 455
ApoI RAATTY 3 cut(s) 142, 200, 534
AspS9I GGNCC 2 cut(s) 76, 635
AsuC2I CCSGG 1 cut(s) 559
AsuHPI GGTGA 2 cut(s) 437, 488
AvaII GGWCC 2 cut(s) 76, 635
BaeGI GKGCMC 1 cut(s) 459
BalI TGGCCA 1 cut(s) 207
BamHI GGATCC 2 cut(s) 70, 554
BanII GRGCYC 1 cut(s) 576
Bbv12I GWGCWC 2 cut(s) 459, 576
BccI CCATC 2 cut(s) 137, 333
BciT130I CCWGG 3 cut(s) 195, 252, 522
BcnI CCSGG 1 cut(s) 559
BcoDI GTCTC 2 cut(s) 195, 335
Bme1390I CCNGG 4 cut(s) 195, 252, 522, 559
Bme18I GGWCC 2 cut(s) 76, 635
BmgT120I GGNCC 2 cut(s) 76, 635
BmiI GGNNCC 3 cut(s) 72, 556, 637
BmrFI CCNGG 4 cut(s) 195, 252, 522, 559
BmsI GCATC 1 cut(s) 192
BpmI CTGGAG 1 cut(s) 468
BpuMI CCSGG 1 cut(s) 559
BsaBI GATNNNNATC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 329
BsaWI WCCGGW 3 cut(s) 67, 73, 374
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 498
Bse3DI GCAATG 1 cut(s) 130
Bse8I GATNNNNATC 1 cut(s) 69
BseAI TCCGGA 2 cut(s) 67, 374
BseBI CCWGG 3 cut(s) 195, 252, 522
BseDI CCNNGG 1 cut(s) 329
BseGI GGATG 3 cut(s) 129, 146, 325
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 2 cut(s) 331, 498
BseMI GCAATG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 459
BshFI GGCC 1 cut(s) 207
BsiHKAI GWGCWC 2 cut(s) 459, 576
BsiSI CCGG 4 cut(s) 68, 74, 375, 558
BslFI GGGAC 1 cut(s) 621
BslI CCNNNNNNNGG 2 cut(s) 331, 498
BsmAI GTCTC 2 cut(s) 195, 335
BsmFI GGGAC 1 cut(s) 621
BsnI GGCC 1 cut(s) 207
Bsp1286I GDGCHC 2 cut(s) 459, 576
Bsp13I TCCGGA 2 cut(s) 67, 374
Bsp143I GATC 6 cut(s) 30, 64, 70, 414, 554, 561
BspANI GGCC 1 cut(s) 207
BspEI TCCGGA 2 cut(s) 67, 374
BspLI GGNNCC 3 cut(s) 72, 556, 637
BspPI GGATC 6 cut(s) 59, 65, 78, 549, 562, 569
BsrDI GCAATG 1 cut(s) 130
BssECI CCNNGG 1 cut(s) 329
BssMI GATC 6 cut(s) 30, 64, 70, 414, 554, 561
Bst2UI CCWGG 3 cut(s) 195, 252, 522
Bst4CI ACNGT 1 cut(s) 160
BstAPI GCANNNNNTGC 2 cut(s) 110, 125
BstC8I GCNNGC 1 cut(s) 121
BstDEI CTNAG 1 cut(s) 603
BstF5I GGATG 3 cut(s) 129, 146, 325
BstKTI GATC 6 cut(s) 33, 67, 73, 417, 557, 564
BstMAI GTCTC 2 cut(s) 195, 335
BstMBI GATC 6 cut(s) 30, 64, 70, 414, 554, 561
BstMWI GCNNNNNNNGC 4 cut(s) 101, 110, 125, 510
BstNI CCWGG 3 cut(s) 195, 252, 522
BstSCI CCNGG 4 cut(s) 193, 250, 520, 557
BstSLI GKGCMC 1 cut(s) 459
BstX2I RGATCY 2 cut(s) 70, 554
BstXI CCANNNNNNTGG 1 cut(s) 521
BstYI RGATCY 2 cut(s) 70, 554
BsuRI GGCC 1 cut(s) 207
BtsCI GGATG 3 cut(s) 129, 146, 325
BtsIMutI CAGTG 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 121
CaiI CAGNNNCTG 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 76, 635
Csp6I GTAC 1 cut(s) 17
CviAII CATG 1 cut(s) 433
CviQI GTAC 1 cut(s) 17
DdeI CTNAG 1 cut(s) 603
DpnI GATC 6 cut(s) 32, 66, 72, 416, 556, 563
DpnII GATC 6 cut(s) 30, 64, 70, 414, 554, 561
EaeI YGGCCR 1 cut(s) 205
Ecl136II GAGCTC 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 576
Eco47I GGWCC 2 cut(s) 76, 635
Eco53kI GAGCTC 1 cut(s) 574
EcoICRI GAGCTC 1 cut(s) 574
EcoRI GAATTC 2 cut(s) 142, 534
EcoRII CCWGG 3 cut(s) 193, 250, 520
EcoT38I GRGCYC 1 cut(s) 576
FaeI CATG 1 cut(s) 436
FaiI YATR 5 cut(s) 48, 92, 175, 434, 494
FaqI GGGAC 1 cut(s) 621
FatI CATG 1 cut(s) 432
FokI GGATG 3 cut(s) 116, 133, 312
FriOI GRGCYC 1 cut(s) 576
GsuI CTGGAG 1 cut(s) 468
HaeIII GGCC 1 cut(s) 207
HapII CCGG 4 cut(s) 68, 74, 375, 558
Hin1II CATG 1 cut(s) 436
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 1 cut(s) 107
HpaII CCGG 4 cut(s) 68, 74, 375, 558
HphI GGTGA 2 cut(s) 437, 488
Hpy166II GTNNAC 3 cut(s) 6, 235, 457
Hpy188I TCNGA 4 cut(s) 64, 284, 330, 554
Hpy188III TCNNGA 2 cut(s) 68, 375
Hpy8I GTNNAC 3 cut(s) 6, 235, 457
HpyAV CCTTC 2 cut(s) 353, 577
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 7 cut(s) 90, 113, 119, 138, 155, 457, 529
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 110, 125, 510
HpyF3I CTNAG 1 cut(s) 603
Hsp92II CATG 1 cut(s) 436
Kpn2I TCCGGA 2 cut(s) 67, 374
Kzo9I GATC 6 cut(s) 30, 64, 70, 414, 554, 561
LmnI GCTCC 1 cut(s) 501
LweI GCATC 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 476
MalI GATC 6 cut(s) 32, 66, 72, 416, 556, 563
MboI GATC 6 cut(s) 30, 64, 70, 414, 554, 561
MboII GAAGA 2 cut(s) 25, 176
MflI RGATCY 2 cut(s) 70, 554
MhlI GDGCHC 2 cut(s) 459, 576
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 4 cut(s) 142, 200, 534, 546
MluNI TGGCCA 1 cut(s) 207
MnlI CCTC 4 cut(s) 47, 324, 327, 421
Mox20I TGGCCA 1 cut(s) 207
MroI TCCGGA 2 cut(s) 67, 374
MscI TGGCCA 1 cut(s) 207
MslI CAYNNNNRTG 2 cut(s) 11, 230
Msp20I TGGCCA 1 cut(s) 207
MspI CCGG 4 cut(s) 68, 74, 375, 558
MspR9I CCNGG 4 cut(s) 195, 252, 522, 559
MvaI CCWGG 3 cut(s) 195, 252, 522
MwoI GCNNNNNNNGC 4 cut(s) 101, 110, 125, 510
NciI CCSGG 1 cut(s) 559
NdeII GATC 6 cut(s) 30, 64, 70, 414, 554, 561
NlaIII CATG 1 cut(s) 436
NlaIV GGNNCC 3 cut(s) 72, 556, 637
NmuCI GTSAC 1 cut(s) 476
PfeI GAWTC 1 cut(s) 107
PfoI TCCNGGA 4 cut(s) 193, 250, 520, 557
Psp124BI GAGCTC 1 cut(s) 576
Psp6I CCWGG 3 cut(s) 193, 250, 520
PspGI CCWGG 3 cut(s) 193, 250, 520
PspN4I GGNNCC 3 cut(s) 72, 556, 637
PspPI GGNCC 2 cut(s) 76, 635
PstNI CAGNNNCTG 1 cut(s) 110
PsuI RGATCY 2 cut(s) 70, 554
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
RseI CAYNNNNRTG 2 cut(s) 11, 230
SacI GAGCTC 1 cut(s) 576
Sau3AI GATC 6 cut(s) 30, 64, 70, 414, 554, 561
Sau96I GGNCC 2 cut(s) 76, 635
ScrFI CCNGG 4 cut(s) 195, 252, 522, 559
SduI GDGCHC 2 cut(s) 459, 576
SetI ASST 6 cut(s) 217, 289, 432, 506, 576, 633
SfaNI GCATC 1 cut(s) 192
SinI GGWCC 2 cut(s) 76, 635
SmiMI CAYNNNNRTG 2 cut(s) 11, 230
Sse9I AATT 4 cut(s) 142, 200, 534, 546
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 4 cut(s) 193, 250, 520, 557
TaaI ACNGT 1 cut(s) 160
TaqI TCGA 1 cut(s) 417
TasI AATT 4 cut(s) 142, 200, 534, 546
TfiI GAWTC 1 cut(s) 107
TscAI CASTG 1 cut(s) 237
TseFI GTSAC 1 cut(s) 476
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 2 cut(s) 135, 482
TspGWI ACGGA 1 cut(s) 353
TspRI CASTG 1 cut(s) 237
VneI GTGCAC 1 cut(s) 455
VpaK11BI GGWCC 2 cut(s) 76, 635
XapI RAATTY 3 cut(s) 142, 200, 534
XcmI CCANNNNNNNNNTGG 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.