pycom02g22780

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011631
Physical Location & Seq
Reverse (-)
20960422 .. 20961442
1021 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
ATGAAACGATTCTTAATATGTTACCAAGTAATAAGTATCTTAATAAGTGACTTTGGTATATCTTACACGGCACTTCGGAAAAGTTCCGGCAAAAACTCGACGATTTTTGAAAGAGGTGAGAGGATTCTTGAAGAAATGAAGAGCAGTGGCATTCTGGTTAACGACACAGTGATTGTGAGTCCATATGGGACGGACAACTTTACATCGATCGGAGATGCCATTACTTCTGCTCCCGAGAAGTTGAAGCTTGAAGATGGCTACTTTGTGATCTATGTTAGAGGAGGATGCTATCATGAGTACATTGTCATCGCCAAACACAAGACTAACATAATGCTGCTCGGAGATGGCATCAATAGCACTGTCATTACCGGAAACCATAGTTTTGTCGATGGTTGGACTACCTTCAACTCCTCAACCTTTGGTAAGAATTACATTTCTGCTAGCTACCTCTTTCTCTTCTGGCCCCCTTGA

Protein Analysis

157

Amino Acids

17.44

Weight (kDa)

6.82

Isoelectric Point (pI)

22.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 57 - 142 1.4e-22 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10720 AT3G10720 AT5G04970
fragaria_vesca FvH4_7g04220
malus_domestica MD02G1266500.v1.1 MD07G1053200.v1.1
prunus_persica Prupe.2G058200_v2.0.a1
pyrus_communis pycom02g22780 pycom02g22790
rosa_chinensis RchiOBHm_Chr1g0330991
rosa_laevigata RLG00000029762
rosa_multiflora Rmu_sc0002001.1_g000004
rosa_roxburghii Rroxscaffold_4G00319570
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113000 Rh1BG087000 Rh1CG109900 Rh1DG118700
rosa_wichuraiana Rw1G009210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 299
AgsI TTSAA 5 cut(s) 110, 131, 244, 251, 406
AluBI AGCT 2 cut(s) 247, 444
AluI AGCT 2 cut(s) 247, 444
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 1 cut(s) 461
ApeKI GCWGC 1 cut(s) 334
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 462
AsuHPI GGTGA 1 cut(s) 128
AsuNHI GCTAGC 1 cut(s) 440
AvaI CYCGRG 1 cut(s) 233
BbvI GCAGC 1 cut(s) 321
BccI CCATC 3 cut(s) 248, 338, 383
BceAI ACGGC 1 cut(s) 84
BfaI CTAG 1 cut(s) 441
BisI GCNGC 1 cut(s) 335
BlsI GCNGC 1 cut(s) 336
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 462
BmiI GGNNCC 1 cut(s) 464
BmsI GCATC 3 cut(s) 205, 275, 357
BmtI GCTAGC 1 cut(s) 444
Bsa29I ATCGAT 1 cut(s) 206
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 2 cut(s) 214, 244
BseCI ATCGAT 1 cut(s) 206
BseGI GGATG 1 cut(s) 290
BseRI GAGGAG 2 cut(s) 294, 400
BseXI GCAGC 1 cut(s) 321
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 1 cut(s) 463
BshVI ATCGAT 1 cut(s) 206
BsiEI CGRYCG 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 2 cut(s) 87, 369
BslFI GGGAC 1 cut(s) 202
BsmFI GGGAC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 150
BsnI GGCC 1 cut(s) 463
BsoBI CYCGRG 1 cut(s) 233
Bsp143I GATC 2 cut(s) 207, 267
BspANI GGCC 1 cut(s) 463
BspDI ATCGAT 1 cut(s) 206
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 464
BspOI GCTAGC 1 cut(s) 444
BspQI GCTCTTC 1 cut(s) 134
BssMI GATC 2 cut(s) 207, 267
Bst4CI ACNGT 2 cut(s) 169, 361
Bst6I CTCTTC 2 cut(s) 134, 461
BstC8I GCNNGC 1 cut(s) 442
BstF5I GGATG 1 cut(s) 290
BstKTI GATC 2 cut(s) 210, 270
BstMBI GATC 2 cut(s) 207, 267
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstV1I GCAGC 1 cut(s) 321
Bsu15I ATCGAT 1 cut(s) 206
BsuRI GGCC 1 cut(s) 463
BsuTUI ATCGAT 1 cut(s) 206
BtgZI GCGATG 1 cut(s) 292
BtsCI GGATG 1 cut(s) 290
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 3 cut(s) 151, 174, 357
Cac8I GCNNGC 1 cut(s) 442
CciI TCATGA 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 462
ClaI ATCGAT 1 cut(s) 206
Csp6I GTAC 1 cut(s) 298
CviAII CATG 1 cut(s) 293
CviJI RGCY 4 cut(s) 247, 258, 444, 463
CviKI_1 RGCY 4 cut(s) 247, 258, 444, 463
CviQI GTAC 1 cut(s) 298
DpnI GATC 2 cut(s) 209, 269
DpnII GATC 2 cut(s) 207, 267
Eam1104I CTCTTC 2 cut(s) 134, 461
EarI CTCTTC 2 cut(s) 134, 461
Eco88I CYCGRG 1 cut(s) 233
FaeI CATG 1 cut(s) 296
FaiI YATR 8 cut(s) 19, 59, 184, 186, 273, 294, 329, 378
FaqI GGGAC 1 cut(s) 202
FatI CATG 1 cut(s) 292
FauNDI CATATG 1 cut(s) 184
Fnu4HI GCNGC 1 cut(s) 335
FokI GGATG 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 335
FspBI CTAG 1 cut(s) 441
GluI GCNGC 1 cut(s) 335
HaeIII GGCC 1 cut(s) 463
HapII CCGG 2 cut(s) 87, 369
Hin1II CATG 1 cut(s) 296
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 3 cut(s) 9, 124, 178
HpaI GTTAAC 1 cut(s) 160
HpaII CCGG 2 cut(s) 87, 369
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 3 cut(s) 78, 212, 341
Hpy188III TCNNGA 3 cut(s) 128, 233, 293
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 1 cut(s) 412
HpyCH4III ACNGT 2 cut(s) 169, 361
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
Hsp92II CATG 1 cut(s) 296
KspAI GTTAAC 1 cut(s) 160
Kzo9I GATC 2 cut(s) 207, 267
LguI GCTCTTC 1 cut(s) 134
LmnI GCTCC 1 cut(s) 235
LpnPI CCDG 4 cut(s) 100, 140, 382, 445
Lsp1109I GCAGC 1 cut(s) 321
LweI GCATC 3 cut(s) 205, 275, 357
MaeI CTAG 1 cut(s) 441
MaeIII GTNAC 2 cut(s) 20, 47
MalI GATC 2 cut(s) 209, 269
MboI GATC 2 cut(s) 207, 267
MboII GAAGA 4 cut(s) 143, 151, 263, 448
MluCI AATT 1 cut(s) 427
MlyI GAGTC 1 cut(s) 187
MmeI TCCRAC 1 cut(s) 374
MnlI CCTC 6 cut(s) 107, 114, 272, 275, 421, 458
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 3 cut(s) 14, 41, 159
MspI CCGG 2 cut(s) 87, 369
Mva1269I GAATGC 1 cut(s) 150
MwoI GCNNNNNNNGC 1 cut(s) 354
NdeI CATATG 1 cut(s) 184
NdeII GATC 2 cut(s) 207, 267
NheI GCTAGC 1 cut(s) 440
NlaIII CATG 1 cut(s) 296
NlaIV GGNNCC 1 cut(s) 464
NmuCI GTSAC 1 cut(s) 47
PagI TCATGA 1 cut(s) 292
PciSI GCTCTTC 1 cut(s) 134
PctI GAATGC 1 cut(s) 150
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 9, 124
PkrI GCNGC 1 cut(s) 336
Ple19I CGATCG 1 cut(s) 210
PleI GAGTC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 464
PspPI GGNCC 1 cut(s) 462
PvuI CGATCG 1 cut(s) 210
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
SapI GCTCTTC 1 cut(s) 134
SaqAI TTAA 3 cut(s) 14, 41, 159
SatI GCNGC 1 cut(s) 335
Sau3AI GATC 2 cut(s) 207, 267
Sau96I GGNCC 1 cut(s) 462
SchI GAGTC 1 cut(s) 187
SetI ASST 6 cut(s) 118, 249, 404, 419, 446, 450
SfaNI GCATC 3 cut(s) 205, 275, 357
Sse9I AATT 1 cut(s) 427
SspMI CTAG 1 cut(s) 441
TaaI ACNGT 2 cut(s) 169, 361
TaqI TCGA 3 cut(s) 98, 206, 387
TasI AATT 1 cut(s) 427
TatI WGTACW 1 cut(s) 297
TfiI GAWTC 2 cut(s) 9, 124
Tru1I TTAA 3 cut(s) 14, 41, 159
Tru9I TTAA 3 cut(s) 14, 41, 159
TscAI CASTG 3 cut(s) 151, 174, 364
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 1 cut(s) 334
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 2 cut(s) 17, 152
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 3 cut(s) 151, 174, 364
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.