pycom02g23800

exoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0015680
Physical Location & Seq
Reverse (-)
21925786 .. 21926602
817 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 750 bp
ATGTTGTTCTGCAAACACTTTAAACTCTTGTCTATTCTTTGTTGTTTGATCTGTGGTGGTTTATGCCAGTTATCCATTGACATGGGTTTGTGTTTCGTCTCATTTTCTTTCTTTCCTGTTGCAGAAGATATGGAAAGGACCTGCGAGGCTTCCCTAAAATGCCTCCACAAGGGATTCCCGTACAACCTCAAGTGTAATGGGAATCCCATTGAAGGATTTCCAGAGCTTAAAGATGAAACTAGTAATCATCCAGTTGGGGATGTTCCTGAATCAGACTGTCCATTGGGCAGCGAATTTCTTGAACCTCCAACTGGATGTTACAACAAACCTACCTACCATCATGATTTTGGATACTGGTCAACTTTCCGTAATGACTCTCAAAAGGTGCAACAGTACCAGATGAATGCTTTTGAGAGCCAGTTTTATCCCTTTCCCATGGAAAATCGATTTCATTATGTTCCATTCAATATGTTTGCTCAAAGTTACCCAAATGACTTCCAGTTCCAAGACTTTCAGTATTTTGTGGTAATAGACTTTGAGGCTACCTGCGATAAGGATAGAAATCCCCATCCTCAAGAGATAATCGAGTTTCCATCTGTGATAGTGAGTAGCATGACAGGCCAACTGGAAGCATGTTTTCAGACTTATGTGAGACCAACATGCAACCAACTCTTGAGTGAGTTCTGTAAGGATCTGACAGGAATCCAGCAAATTCAGGCTGTATTCAGTGACATTATCCTAATTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006139 GO:0006259 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0071704 GO:0090304 GO:1901360
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T zf-GRF

Protein Analysis

250

Amino Acids

28.72

Weight (kDa)

4.7

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 175 - 238 3.5e-09 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g03700
malus_domestica MD02G1278000.v1.1 MD07G1045900.v1.1
prunus_persica Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1
pyrus_communis pycom02g23800 pycom07g03310
rosa_chinensis RchiOBHm_Chr1g0326491
rosa_laevigata RLG00000030069
rosa_rugosa Rorug01G0061000
rosa_samantha Rh1AG076600 Rh1BG062500 Rh1CG075300 Rh1DG082000
rosa_wichuraiana Rw1G006120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 149, 554
AclWI GGATC 1 cut(s) 699
AcsI RAATTY 2 cut(s) 293, 711
AfaI GTAC 2 cut(s) 182, 395
AfiI CCNNNNNNNGG 3 cut(s) 169, 212, 311
AgsI TTSAA 3 cut(s) 212, 302, 466
AhlI ACTAGT 1 cut(s) 239
AluBI AGCT 1 cut(s) 226
AluI AGCT 1 cut(s) 226
Alw26I GTCTC 2 cut(s) 103, 646
AlwI GGATC 1 cut(s) 699
AoxI GGCC 1 cut(s) 619
ApeKI GCWGC 1 cut(s) 288
ApoI RAATTY 2 cut(s) 293, 711
Asp700I GAANNNNTTC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 138
AvaII GGWCC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 300
BccI CCATC 3 cut(s) 345, 576, 601
BciVI GTATCC 1 cut(s) 344
BcoDI GTCTC 2 cut(s) 103, 646
BcuI ACTAGT 1 cut(s) 239
BfaI CTAG 1 cut(s) 240
BfuAI ACCTGC 2 cut(s) 149, 554
BfuI GTATCC 1 cut(s) 344
BisI GCNGC 1 cut(s) 289
BlsI GCNGC 1 cut(s) 290
Bme18I GGWCC 1 cut(s) 138
BmgT120I GGNCC 1 cut(s) 138
BpuEI CTTGAG 3 cut(s) 173, 558, 694
Bsa29I ATCGAT 1 cut(s) 445
BsaBI GATNNNNATC 1 cut(s) 561
BsaI GGTCTC 1 cut(s) 646
BsaJI CCNNGG 1 cut(s) 435
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 212, 311
Bse1I ACTGG 7 cut(s) 67, 251, 316, 359, 418, 499, 630
Bse8I GATNNNNATC 1 cut(s) 561
BseCI ATCGAT 1 cut(s) 445
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 4 cut(s) 247, 265, 320, 568
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 3 cut(s) 169, 212, 311
BseNI ACTGG 7 cut(s) 67, 251, 316, 359, 418, 499, 630
BseXI GCAGC 1 cut(s) 300
BshFI GGCC 1 cut(s) 621
BshVI ATCGAT 1 cut(s) 445
BslI CCNNNNNNNGG 3 cut(s) 169, 212, 311
BsmAI GTCTC 2 cut(s) 103, 646
BsmBI CGTCTC 1 cut(s) 103
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 1 cut(s) 621
Bso31I GGTCTC 1 cut(s) 646
Bsp143I GATC 2 cut(s) 48, 691
Bsp19I CCATGG 1 cut(s) 435
BspANI GGCC 1 cut(s) 621
BspDI ATCGAT 1 cut(s) 445
BspHI TCATGA 1 cut(s) 340
BspMI ACCTGC 2 cut(s) 149, 554
BspPI GGATC 1 cut(s) 699
BspTNI GGTCTC 1 cut(s) 646
BsrI ACTGG 7 cut(s) 67, 251, 316, 359, 418, 499, 630
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 2 cut(s) 48, 691
BssT1I CCWWGG 1 cut(s) 435
Bst4CI ACNGT 2 cut(s) 278, 393
BstDSI CCRYGG 1 cut(s) 435
BstENI CCTNNNNNAGG 1 cut(s) 167
BstF5I GGATG 4 cut(s) 247, 265, 320, 568
BstKTI GATC 2 cut(s) 51, 694
BstMAI GTCTC 2 cut(s) 103, 646
BstMBI GATC 2 cut(s) 48, 691
BstMWI GCNNNNNNNGC 1 cut(s) 618
BstNSI RCATGY 2 cut(s) 636, 663
BstV1I GCAGC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 691
BstXI CCANNNNNNTGG 1 cut(s) 82
BstYI RGATCY 1 cut(s) 691
Bsu15I ATCGAT 1 cut(s) 445
BsuI GTATCC 1 cut(s) 344
BsuRI GGCC 1 cut(s) 621
BsuTUI ATCGAT 1 cut(s) 445
BtgI CCRYGG 1 cut(s) 435
BtsCI GGATG 4 cut(s) 247, 265, 320, 568
BtsIMutI CAGTG 1 cut(s) 733
BveI ACCTGC 2 cut(s) 149, 554
CciI TCATGA 1 cut(s) 340
Cfr13I GGNCC 1 cut(s) 138
ClaI ATCGAT 1 cut(s) 445
Csp6I GTAC 2 cut(s) 181, 394
CviAII CATG 6 cut(s) 82, 341, 436, 613, 633, 660
CviJI RGCY 6 cut(s) 149, 226, 417, 542, 621, 719
CviKI_1 RGCY 6 cut(s) 149, 226, 417, 542, 621, 719
CviQI GTAC 2 cut(s) 181, 394
DpnI GATC 2 cut(s) 50, 693
DpnII GATC 2 cut(s) 48, 691
DraI TTTAAA 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 435
Eco31I GGTCTC 1 cut(s) 646
Eco47I GGWCC 1 cut(s) 138
EcoNI CCTNNNNNAGG 1 cut(s) 167
EcoO109I RGGNCCY 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 6 cut(s) 85, 344, 439, 616, 636, 663
FatI CATG 6 cut(s) 81, 340, 435, 612, 632, 659
Fnu4HI GCNGC 1 cut(s) 289
FokI GGATG 4 cut(s) 234, 272, 327, 555
Fsp4HI GCNGC 1 cut(s) 289
FspBI CTAG 1 cut(s) 240
GluI GCNGC 1 cut(s) 289
HaeIII GGCC 1 cut(s) 621
Hin1II CATG 6 cut(s) 85, 344, 439, 616, 636, 663
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HinfI GANTC 5 cut(s) 174, 202, 269, 374, 702
Hpy166II GTNNAC 1 cut(s) 360
Hpy188I TCNGA 4 cut(s) 274, 642, 696, 749
Hpy188III TCNNGA 6 cut(s) 221, 266, 299, 341, 575, 673
Hpy8I GTNNAC 1 cut(s) 360
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 2 cut(s) 278, 393
HpyCH4V TGCA 4 cut(s) 12, 122, 388, 663
HpyF10VI GCNNNNNNNGC 1 cut(s) 618
Hsp92II CATG 6 cut(s) 85, 344, 439, 616, 636, 663
Kzo9I GATC 2 cut(s) 48, 691
Lsp1109I GCAGC 1 cut(s) 300
MaeI CTAG 1 cut(s) 240
MaeIII GTNAC 3 cut(s) 317, 482, 728
MalI GATC 2 cut(s) 50, 693
MboI GATC 2 cut(s) 48, 691
MboII GAAGA 1 cut(s) 137
MflI RGATCY 1 cut(s) 691
MluCI AATT 3 cut(s) 293, 711, 741
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 1 cut(s) 332
MnlI CCTC 6 cut(s) 139, 173, 197, 315, 532, 582
MroXI GAANNNNTTC 1 cut(s) 216
MseI TTAA 2 cut(s) 21, 228
MslI CAYNNNNRTG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 409
MwoI GCNNNNNNNGC 1 cut(s) 618
NcoI CCATGG 1 cut(s) 435
NdeII GATC 2 cut(s) 48, 691
NlaIII CATG 6 cut(s) 85, 344, 439, 616, 636, 663
NmuCI GTSAC 1 cut(s) 728
NspI RCATGY 2 cut(s) 636, 663
PagI TCATGA 1 cut(s) 340
PctI GAATGC 1 cut(s) 409
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 4 cut(s) 174, 202, 269, 702
PkrI GCNGC 1 cut(s) 290
PleI GAGTC 1 cut(s) 368
PpsI GAGTC 1 cut(s) 368
PpuMI RGGWCCY 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 138
PspPI GGNCC 1 cut(s) 138
PspPPI RGGWCCY 1 cut(s) 138
PsuI RGATCY 1 cut(s) 691
RsaI GTAC 2 cut(s) 182, 395
RsaNI GTAC 2 cut(s) 181, 394
RseI CAYNNNNRTG 1 cut(s) 80
SaqAI TTAA 2 cut(s) 21, 228
SatI GCNGC 1 cut(s) 289
Sau3AI GATC 2 cut(s) 48, 691
Sau96I GGNCC 1 cut(s) 138
SchI GAGTC 1 cut(s) 368
SetI ASST 8 cut(s) 143, 189, 228, 307, 331, 335, 387, 548
SinI GGWCC 1 cut(s) 138
SmiMI CAYNNNNRTG 1 cut(s) 80
SmlI CTYRAG 3 cut(s) 188, 573, 673
SmoI CTYRAG 3 cut(s) 188, 573, 673
SpeI ACTAGT 1 cut(s) 239
Sse9I AATT 3 cut(s) 293, 711, 741
SspMI CTAG 1 cut(s) 240
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 2 cut(s) 278, 393
TaqI TCGA 2 cut(s) 445, 585
TasI AATT 3 cut(s) 293, 711, 741
TfiI GAWTC 4 cut(s) 174, 202, 269, 702
Tru1I TTAA 2 cut(s) 21, 228
Tru9I TTAA 2 cut(s) 21, 228
TscAI CASTG 1 cut(s) 733
TseFI GTSAC 1 cut(s) 728
TseI GCWGC 1 cut(s) 288
Tsp45I GTSAC 1 cut(s) 728
TspDTI ATGAA 3 cut(s) 249, 416, 440
TspGWI ACGGA 1 cut(s) 356
TspRI CASTG 1 cut(s) 733
VpaK11BI GGWCC 1 cut(s) 138
XagI CCTNNNNNAGG 1 cut(s) 167
XapI RAATTY 2 cut(s) 293, 711
XceI RCATGY 2 cut(s) 636, 663
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.