Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0007312

Physical Location & Seq

Genomic Coordinates Reverse (-)

23810902 .. 23813100

Gene Structure 2199 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g25910.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2199 bp

ATGTATAAAATACCAAATATTTTGACCCCTCTGACAAGAAATTTGCAATCTTTGTTAAAACCCCACTGTTTTGCAATTTTGTCAGCTGGGTATTTTAAGGATGTTAGTGATAGAGTGTTGAATGATAAGGGGTCAGAGTCAGAAAATGAGTGGGAGAAGATTCTTAAGCCCTTTGACCTCAATGAACTTAGGAAGTCACTTGTTCAAATCACTCCAATTCAACTTTGTAAGTTGCTTCAACTTCCACTCGATGTGCCCTCGTCGATGGAGATATTTGAGTTGGTTGGCGCCCAGAAGGGGTATTGCCATTCTTTTGATGTGTACTATGTGTTAATTGATAAGCTCGGGGCAGCCGGGGAATTTAAAATTATAGATAGGTTGTTGATGCAGATTAAAGAGAAGGGGTTTAATTTTAGTGAGTCCCTTTTCATTATGATTATGAAGTATTATGGGAGGGCTGGTTTGCCCGGACAAGCGACCAGGCTGCTTTTAGACATGAGAGGTGTTTATTCATGTGAACCGACTTTTAGATCGTATAATGTTGTATTGGATATCCTTGTGGCTGGGAATTGCCCTAAGGTTGCTCCCAATGTCTTCTATGACATGTTGAGTAGGGGTATCTCTCCCAATGTTTACACGTTTGGAGTGGTTCTGAAAGCACTTTGTATGGTTAATGAGGTGGATTCCGCTTGCTCACTTCTAAAAGACATGACGAAGCATGGTTGTGTACCAAATTCAGTGGTTTACCAGACTCTTATACATGCACTCTCCAAGAATAATCAAGTGAATGATGCATTGAGACTTTTGGAGGAAATGTTTTTGATGGGTTGTACACCTGATGTTCAGACTTTCAATGATATTATCCATGGTCTTTGCAAGGTCAATCGGATTCATGAGGCAGCAAGATTGGTTGATCGGATGCTTCTCCGGGGGTTTACTCCTGATGATGTGACTTATGGAGTTTTAATGCATGGATTATGCAGAACAGGGCAAGTTGATGAAGCAAAGGTATTGTTGAACAAAGTGCCTTCCCCAAATATTGTGCTTTTCAATACCTTGATTAATGGTTATGTTATGAGTGGACGGTTTGATGAAGCCAAAGCTGTTCTATATGATGGTATGTTAGGTAATGGCTGCAACCCTGATGTATATACATTTAACATTCTGATTCATGGGCTCTGCAAGAAGAGCTGTTTGAGTTCTGCTCGTGAATTGGTCAGTGAGATGGAAATTAAGGGCTGCAAGCCTAATGTAATAACTTACACCATAATGATCGATGGGTTCTGCAAGGAGGGCCAACTGGAGGAAGCTGGTGATGTTTTAAATGAGATGTCATACAAGGGACTAAGTCTGAATACCATGGGATACAATCGTCTAATTTCTGCTCTATGCAAGGATGGGAAAGTCCAGGAAGCTTTAAAATTGTTTTGCGAAATCTCAAGCAAAGGATGTAAGCCAGACATATTTACGTTTAATTCTTTAATATTTGGACTGTGCAAGGTTGATCAAATGGAAGAGGCATTGGGATTGTACCGTGATATGTTACTGGAGGGTGTTGTTGCCAACACTGTTACTTACAACACGTTAATTAATGCTTTCTTGACAAGAGGTGCGATACAAGAAGCACTTAAGCTTGTAAATGAAATGGTATTTAGAGGATGCCCTCTCGACAAAATAACCTATAATGGGCTAATTAAGGCACTCTGCAAGGCTGGAGCCGTTGACAAGGCAAGGGGATTGTTTGAAGAAATGATGATGAAGGGACTTCATCATGATAGTTTCTCCTGTAATATTTTGATTAATGGTATGTGTAAAAGTGGCAAAGTATATGATGCACTTGAGTTTCTACGAGATATGATTCATCGGGGTTTGATGCCAGACATAGTCACTTATAATAGCCTCATAAATGGTCTATGCAAGATGGGACACATTTGGGAAGCTTTGAATCTCTTTGATAGATTACAAGCTGAAGGGATGTGTCCTGATGTTATTACTTATAACACTCTGATCAGTTGGCACTGCAAACAGGGAATGATTGATGATGCTTGTTTACTTCTACATAGAGGCGTGAATAATGGGTTGATACCAAATCATTGGACTTGGTATATACTGGTCAGCAACTTGTCTAAAGAAAGGGGAGAGGTGAATCAGAGCTTTATTGGTATTGATCGGGAGGAATCTTATTTTAGTTTCTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0019538 GO:0032543 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:0140053 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

733

Amino Acids

82.18

Weight (kDa)

6.24

Isoelectric Point (pI)

27.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	178 - 222	7e-09	PPR repeat family
TPR_24	PF23276	191 - 302	1.9e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	198 - 258	8e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	205 - 238	3.3e-06	PPR repeat
PPR_2	PF13041	217 - 258	5.4e-09	PPR repeat family
PPR_long	PF17177	223 - 340	8.8e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	232 - 289	1.9e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	241 - 272	4.5e-09	PPR repeat
PPR_2	PF13041	244 - 293	5.1e-15	PPR repeat family
PPR_1	PF12854	275 - 307	2.2e-10	PPR repeat
PPR_2	PF13041	279 - 328	1.3e-14	PPR repeat family
PPR_1	PF12854	310 - 341	1.4e-08	PPR repeat
PPR_2	PF13041	345 - 395	1.2e-15	PPR repeat family
PPR_3	PF13812	373 - 427	4.9e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	377 - 410	2.8e-11	PPR repeat
PPR_2	PF13041	386 - 430	4.4e-16	PPR repeat family
TPR_24	PF23276	412 - 514	1.1e-08	Fungal tetratrico peptide repeats
PPR_1	PF12854	412 - 445	7.7e-15	PPR repeat
PPR	PF01535	419 - 449	1.8e-08	PPR repeat
PPR_3	PF13812	440 - 495	3.8e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	447 - 478	6.5e-08	PPR repeat
PPR_2	PF13041	452 - 500	3.1e-17	PPR repeat family
PPR	PF01535	456 - 484	2.7e-07	PPR repeat
PPR_long	PF17177	465 - 620	5.2e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	482 - 514	3e-10	PPR repeat
PPR_2	PF13041	486 - 526	2e-10	PPR repeat family
PPR	PF01535	489 - 519	1.2e-06	PPR repeat
PPR_1	PF12854	518 - 550	3.3e-07	PPR repeat
TPR_24	PF23276	518 - 617	1.1e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	522 - 570	1.5e-17	PPR repeat family
PPR	PF01535	524 - 554	8.9e-06	PPR repeat
PPR_1	PF12854	552 - 584	2.3e-11	PPR repeat
PPR	PF01535	559 - 589	8.4e-08	PPR repeat
PPR_2	PF13041	559 - 605	1.1e-12	PPR repeat family
PPR_3	PF13812	580 - 636	5.9e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	587 - 619	4.3e-07	PPR repeat
PPR_2	PF13041	592 - 640	2.7e-17	PPR repeat family
PPR	PF01535	594 - 624	3e-06	PPR repeat
PPR_3	PF13812	616 - 672	6.9e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	622 - 653	4e-13	PPR repeat
PPR_2	PF13041	626 - 671	1.3e-14	PPR repeat family
PPR	PF01535	629 - 658	4.4e-08	PPR repeat
PPR_1	PF12854	658 - 687	1.6e-10	PPR repeat
PPR_2	PF13041	661 - 710	4.2e-10	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007312)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64320
fragaria_vesca	FvH4_5g29651 FvH4_5g29651
malus_domestica	MD02G1307900.v1.1
prunus_persica	Prupe.4G007800_v2.0.a1 Prupe.4G007800_v2.0.a1 Prupe.4G007800_v2.0.a1 Prupe.4G007800_v2.0.a1 Prupe.4G007800_v2.0.a1 Prupe.4G007800_v2.0.a1 Prupe.4G007800_v2.0.a1
pyrus_communis	pycom02g25910
rosa_chinensis	RchiOBHm_Chr7g0222741
rosa_laevigata	RLG00000002027
rosa_multiflora	Rmu_sc0007870.1_g000004 Rmu_sc0025009.1_g000002
rosa_roxburghii	Rroxscaffold_3G00236510
rosa_rugosa	Rorug07G0212100 Rorug07G0212200 Rorug07G0212200
rosa_samantha	Rh7AG356400 Rh7BG344800 Rh7CG373900 Rh7DG350700
rosa_wichuraiana	Rw7G030240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1893, 1998
AccB1I	GGYRCC	1 cut(s)	287
AciI	CCGC	1 cut(s)	687
AcsI	RAATTY	3 cut(s)	40, 359, 733
AcuI	CTGAAG	1 cut(s)	1989
AcyI	GRCGYC	1 cut(s)	288
AfaI	GTAC	4 cut(s)	323, 729, 832, 1532
AfiI	CCNNNNNNNGG	3 cut(s)	297, 1303, 1686
AflII	CTTAAG	2 cut(s)	164, 1628
AflIII	ACRYGT	3 cut(s)	603, 636, 1581
AgsI	TTSAA	9 cut(s)	121, 206, 221, 239, 853, 1018, 1051, 1745, 1945
AjnI	CCWGG	2 cut(s)	479, 1407
Alw26I	GTCTC	1 cut(s)	793
Ama87I	CYCGRG	1 cut(s)	344
AoxI	GGCC	1 cut(s)	1294
ApeKI	GCWGC	5 cut(s)	350, 484, 899, 1134, 1239
ApoI	RAATTY	3 cut(s)	40, 359, 733
ArsI	GACNNNNNNTTYG	2 cut(s)	25, 57
AseI	ATTAAT	3 cut(s)	1062, 1590, 1800
AspLEI	GCGC	1 cut(s)	290
AspS9I	GGNCC	1 cut(s)	1294
AsuC2I	CCSGG	3 cut(s)	355, 468, 929
AsuHPI	GGTGA	2 cut(s)	1325, 2155
AvaI	CYCGRG	1 cut(s)	344
AxyI	CCTNAGG	1 cut(s)	576
BaeGI	GKGCMC	1 cut(s)	258
BaeI	ACNNNNGTAYC	2 cut(s)	1357, 1390
BanI	GGYRCC	1 cut(s)	287
BanII	GRGCYC	1 cut(s)	1179
BauI	CACGAG	1 cut(s)	1206
BbsI	GAAGAC	1 cut(s)	586
BbvI	GCAGC	5 cut(s)	362, 471, 911, 1121, 1226
BccI	CCATC	7 cut(s)	259, 817, 1109, 1219, 1271, 1391, 1915
BceAI	ACGGC	1 cut(s)	1703
BcgI	CGANNNNNNTGC	2 cut(s)	466, 500
BciT130I	CCWGG	2 cut(s)	481, 1409
BciVI	GTATCC	1 cut(s)	1358
BclI	TGATCA	2 cut(s)	1504, 2007
BcnI	CCSGG	3 cut(s)	355, 468, 929
BcoDI	GTCTC	1 cut(s)	793
BfaI	CTAG	1 cut(s)	2197
BfoI	RGCGCY	1 cut(s)	291
BfrI	CTTAAG	2 cut(s)	164, 1628
BfuI	GTATCC	1 cut(s)	1358
BisI	GCNGC	5 cut(s)	351, 485, 900, 1135, 1240
BlsI	GCNGC	5 cut(s)	352, 486, 901, 1136, 1241
Bme1390I	CCNGG	5 cut(s)	355, 468, 481, 929, 1409
BmeT110I	CYCGRG	1 cut(s)	344
BmgT120I	GGNCC	1 cut(s)	1294
BmiI	GGNNCC	2 cut(s)	289, 1717
BmrFI	CCNGG	5 cut(s)	355, 468, 481, 929, 1409
BmsI	GCATC	7 cut(s)	375, 781, 909, 1649, 1822, 1863, 2032
BpiI	GAAGAC	1 cut(s)	586
BplI	GAGNNNNNCTC	2 cut(s)	1189, 1221
BpmI	CTGGAG	3 cut(s)	1322, 1568, 1734
BpuEI	CTTGAG	2 cut(s)	1423, 1859
BpuMI	CCSGG	3 cut(s)	355, 468, 929
Bsa29I	ATCGAT	1 cut(s)	1275
BsaHI	GRCGYC	1 cut(s)	288
BsaJI	CCNNGG	4 cut(s)	354, 865, 928, 1359
BsaXI	ACNNNNNCTCC	4 cut(s)	445, 475, 800, 830
Bsc4I	CCNNNNNNNGG	3 cut(s)	297, 1303, 1686
Bse1I	ACTGG	3 cut(s)	1305, 1551, 2115
Bse21I	CCTNAGG	1 cut(s)	576
BseBI	CCWGG	2 cut(s)	481, 1409
BseCI	ATCGAT	1 cut(s)	1275
BseDI	CCNNGG	4 cut(s)	354, 865, 928, 1359
BseGI	GGATG	6 cut(s)	106, 924, 1402, 1454, 1664, 1980
BseLI	CCNNNNNNNGG	3 cut(s)	297, 1303, 1686
BseNI	ACTGG	3 cut(s)	1305, 1551, 2115
BseSI	GKGCMC	1 cut(s)	258
BseXI	GCAGC	5 cut(s)	362, 471, 911, 1121, 1226
BseYI	CCCAGC	2 cut(s)	86, 563
BshFI	GGCC	1 cut(s)	1296
BshNI	GGYRCC	1 cut(s)	287
BshVI	ATCGAT	1 cut(s)	1275
BsiHKCI	CYCGRG	1 cut(s)	344
BsiSI	CCGG	3 cut(s)	354, 468, 928
BslFI	GGGAC	4 cut(s)	406, 1356, 1776, 1938
BslI	CCNNNNNNNGG	3 cut(s)	297, 1303, 1686
BsmAI	GTCTC	1 cut(s)	793
BsmFI	GGGAC	4 cut(s)	406, 1356, 1776, 1938
BsnI	GGCC	1 cut(s)	1296
BsoBI	CYCGRG	1 cut(s)	344
Bsp1286I	GDGCHC	2 cut(s)	258, 1179
Bsp1407I	TGTACA	1 cut(s)	830
Bsp143I	GATC	6 cut(s)	530, 913, 1272, 1504, 2007, 2167
Bsp19I	CCATGG	2 cut(s)	865, 1359
BspACI	CCGC	1 cut(s)	687
BspANI	GGCC	1 cut(s)	1296
BspDI	ATCGAT	1 cut(s)	1275
BspHI	TCATGA	2 cut(s)	892, 1771
BspLI	GGNNCC	2 cut(s)	289, 1717
BspQI	GCTCTTC	1 cut(s)	1181
BspT107I	GGYRCC	1 cut(s)	287
BspTI	CTTAAG	2 cut(s)	164, 1628
BsrGI	TGTACA	1 cut(s)	830
BsrI	ACTGG	3 cut(s)	1305, 1551, 2115
BssECI	CCNNGG	4 cut(s)	354, 865, 928, 1359
BssMI	GATC	6 cut(s)	530, 913, 1272, 1504, 2007, 2167
BssNI	GRCGYC	1 cut(s)	288
BssSI	CACGAG	1 cut(s)	1206
BssT1I	CCWWGG	2 cut(s)	865, 1359
Bst2BI	CACGAG	1 cut(s)	1206
Bst2UI	CCWGG	2 cut(s)	481, 1409
Bst4CI	ACNGT	5 cut(s)	68, 1086, 1494, 1535, 1570
Bst6I	CTCTTC	2 cut(s)	1181, 1509
BstACI	GRCGYC	1 cut(s)	288
BstAFI	CTTAAG	2 cut(s)	164, 1628
BstAUI	TGTACA	1 cut(s)	830
BstC8I	GCNNGC	2 cut(s)	691, 1244
BstDEI	CTNAG	3 cut(s)	188, 576, 1346
BstDSI	CCRYGG	2 cut(s)	865, 1359
BstF5I	GGATG	6 cut(s)	106, 924, 1402, 1454, 1664, 1980
BstH2I	RGCGCY	1 cut(s)	291
BstHHI	GCGC	1 cut(s)	290
BstKTI	GATC	6 cut(s)	533, 916, 1275, 1507, 2010, 2170
BstMAI	GTCTC	1 cut(s)	793
BstMBI	GATC	6 cut(s)	530, 913, 1272, 1504, 2007, 2167
BstMWI	GCNNNNNNNGC	2 cut(s)	1188, 1293
BstNI	CCWGG	2 cut(s)	481, 1409
BstNSI	RCATGY	2 cut(s)	607, 764
BstSCI	CCNGG	5 cut(s)	353, 466, 479, 927, 1407
BstSLI	GKGCMC	1 cut(s)	258
BstV1I	GCAGC	5 cut(s)	362, 471, 911, 1121, 1226
BstV2I	GAAGAC	1 cut(s)	586
BstXI	CCANNNNNNTGG	1 cut(s)	2094
Bsu15I	ATCGAT	1 cut(s)	1275
Bsu36I	CCTNAGG	1 cut(s)	576
BsuI	GTATCC	1 cut(s)	1358
BsuRI	GGCC	1 cut(s)	1296
BsuTUI	ATCGAT	1 cut(s)	1275
BtgI	CCRYGG	2 cut(s)	865, 1359
BtsCI	GGATG	6 cut(s)	106, 924, 1402, 1454, 1664, 1980
BtsI	GCAGTG	1 cut(s)	2017
BtsIMutI	CAGTG	5 cut(s)	64, 744, 1225, 1566, 2017
Cac8I	GCNNGC	2 cut(s)	691, 1244
CciI	TCATGA	2 cut(s)	892, 1771
CfoI	GCGC	1 cut(s)	290
Cfr13I	GGNCC	1 cut(s)	1294
ClaI	ATCGAT	1 cut(s)	1275
Csp6I	GTAC	4 cut(s)	322, 728, 831, 1531
CspCI	CAANNNNNGTGG	2 cut(s)	720, 755
CviQI	GTAC	4 cut(s)	322, 728, 831, 1531
DdeI	CTNAG	3 cut(s)	188, 576, 1346
DinI	GGCGCC	1 cut(s)	289
DpnI	GATC	6 cut(s)	532, 915, 1274, 1506, 2009, 2169
DpnII	GATC	6 cut(s)	530, 913, 1272, 1504, 2007, 2167
DraI	TTTAAA	3 cut(s)	364, 1323, 1419
Eam1104I	CTCTTC	2 cut(s)	1181, 1509
EarI	CTCTTC	2 cut(s)	1181, 1509
Eco130I	CCWWGG	2 cut(s)	865, 1359
Eco24I	GRGCYC	1 cut(s)	1179
Eco32I	GATATC	1 cut(s)	553
Eco57I	CTGAAG	1 cut(s)	1989
Eco81I	CCTNAGG	1 cut(s)	576
Eco88I	CYCGRG	1 cut(s)	344
EcoRII	CCWGG	2 cut(s)	479, 1407
EcoRV	GATATC	1 cut(s)	553
EcoT14I	CCWWGG	2 cut(s)	865, 1359
EcoT22I	ATGCAT	2 cut(s)	796, 972
EcoT38I	GRGCYC	1 cut(s)	1179
EgeI	GGCGCC	1 cut(s)	289
EheI	GGCGCC	1 cut(s)	289
ErhI	CCWWGG	2 cut(s)	865, 1359
FalI	AAGNNNNNCTT	2 cut(s)	1611, 1643
FaqI	GGGAC	4 cut(s)	406, 1356, 1776, 1938
FbaI	TGATCA	2 cut(s)	1504, 2007
Fnu4HI	GCNGC	5 cut(s)	351, 485, 900, 1135, 1240
FokI	GGATG	6 cut(s)	113, 931, 1409, 1461, 1671, 1987
FriOI	GRGCYC	1 cut(s)	1179
Fsp4HI	GCNGC	5 cut(s)	351, 485, 900, 1135, 1240
FspBI	CTAG	1 cut(s)	2197
GlaI	GCGC	1 cut(s)	289
GluI	GCNGC	5 cut(s)	351, 485, 900, 1135, 1240
GsaI	CCCAGC	2 cut(s)	90, 567
GsuI	CTGGAG	3 cut(s)	1322, 1568, 1734
HaeII	RGCGCY	1 cut(s)	291
HaeIII	GGCC	1 cut(s)	1296
HapII	CCGG	3 cut(s)	354, 468, 928
HhaI	GCGC	1 cut(s)	290
Hin1I	GRCGYC	1 cut(s)	288
Hin6I	GCGC	1 cut(s)	288
HinP1I	GCGC	1 cut(s)	288
HincII	GTYRAC	1 cut(s)	1723
HindII	GTYRAC	1 cut(s)	1723
HindIII	AAGCTT	3 cut(s)	1413, 1631, 1938
HpaII	CCGG	3 cut(s)	354, 468, 928
HphI	GGTGA	2 cut(s)	1325, 2155
Hpy188III	TCNNGA	8 cut(s)	893, 941, 1208, 1600, 1667, 1772, 1982, 2171
Hpy99I	CGWCG	1 cut(s)	265
HpyAV	CCTTC	5 cut(s)	289, 394, 1038, 1753, 1964
HpyCH4III	ACNGT	5 cut(s)	68, 1086, 1494, 1535, 1570
HpyCH4IV	ACGT	3 cut(s)	638, 1469, 1583
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1188, 1293
HpyF3I	CTNAG	3 cut(s)	188, 576, 1346
HpySE526I	ACGT	3 cut(s)	638, 1469, 1583
Hsp92I	GRCGYC	1 cut(s)	288
HspAI	GCGC	1 cut(s)	288
KasI	GGCGCC	1 cut(s)	287
Ksp22I	TGATCA	2 cut(s)	1504, 2007
Kzo9I	GATC	6 cut(s)	530, 913, 1272, 1504, 2007, 2167
LguI	GCTCTTC	1 cut(s)	1181
LmnI	GCTCC	2 cut(s)	589, 1715
Lsp1109I	GCAGC	5 cut(s)	362, 471, 911, 1121, 1226
LweI	GCATC	7 cut(s)	375, 781, 909, 1649, 1822, 1863, 2032
MaeI	CTAG	1 cut(s)	2197
MaeII	ACGT	3 cut(s)	638, 1469, 1583
MaeIII	GTNAC	5 cut(s)	195, 949, 1542, 1570, 1885
MalI	GATC	6 cut(s)	532, 915, 1274, 1506, 2009, 2169
MboI	GATC	6 cut(s)	530, 913, 1272, 1504, 2007, 2167
MboII	GAAGA	6 cut(s)	169, 586, 1198, 1526, 1757, 2185
MhlI	GDGCHC	2 cut(s)	258, 1179
Mly113I	GGCGCC	1 cut(s)	288
MlyI	GAGTC	3 cut(s)	146, 428, 745
Mph1103I	ATGCAT	2 cut(s)	796, 972
MslI	CAYNNNNRTG	2 cut(s)	723, 1268
MspA1I	CMGCKG	1 cut(s)	86
MspCI	CTTAAG	2 cut(s)	164, 1628
MspI	CCGG	3 cut(s)	354, 468, 928
MspR9I	CCNGG	5 cut(s)	355, 468, 481, 929, 1409
MvaI	CCWGG	2 cut(s)	481, 1409
MwoI	GCNNNNNNNGC	2 cut(s)	1188, 1293
NarI	GGCGCC	1 cut(s)	288
NciI	CCSGG	3 cut(s)	355, 468, 929
NcoI	CCATGG	2 cut(s)	865, 1359
NdeII	GATC	6 cut(s)	530, 913, 1272, 1504, 2007, 2167
NlaIV	GGNNCC	2 cut(s)	289, 1717
NmuCI	GTSAC	3 cut(s)	195, 949, 1885
NsiI	ATGCAT	2 cut(s)	796, 972
NspI	RCATGY	2 cut(s)	607, 764
PacI	TTAATTAA	1 cut(s)	1590
PagI	TCATGA	2 cut(s)	892, 1771
PciI	ACATGT	1 cut(s)	603
PciSI	GCTCTTC	1 cut(s)	1181
PfeI	GAWTC	8 cut(s)	160, 683, 889, 1168, 1858, 1945, 2146, 2177
PflFI	GACNNNGTC	2 cut(s)	1347, 1883
PfoI	TCCNGGA	1 cut(s)	1407
PkrI	GCNGC	5 cut(s)	352, 486, 901, 1136, 1241
PleI	GAGTC	3 cut(s)	145, 427, 745
PluTI	GGCGCC	1 cut(s)	291
PpsI	GAGTC	3 cut(s)	145, 427, 745
PscI	ACATGT	1 cut(s)	603
PshBI	ATTAAT	3 cut(s)	1062, 1590, 1800
PsiI	TTATAA	2 cut(s)	1893, 1998
Psp6I	CCWGG	2 cut(s)	479, 1407
PspFI	CCCAGC	2 cut(s)	86, 563
PspGI	CCWGG	2 cut(s)	479, 1407
PspN4I	GGNNCC	2 cut(s)	289, 1717
PspPI	GGNCC	1 cut(s)	1294
PsyI	GACNNNGTC	2 cut(s)	1347, 1883
PvuII	CAGCTG	1 cut(s)	86
RsaI	GTAC	4 cut(s)	323, 729, 832, 1532
RsaNI	GTAC	4 cut(s)	322, 728, 831, 1531
RseI	CAYNNNNRTG	2 cut(s)	723, 1268
SapI	GCTCTTC	1 cut(s)	1181
SatI	GCNGC	5 cut(s)	351, 485, 900, 1135, 1240
Sau3AI	GATC	6 cut(s)	530, 913, 1272, 1504, 2007, 2167
Sau96I	GGNCC	1 cut(s)	1294
SchI	GAGTC	3 cut(s)	146, 428, 745
ScrFI	CCNGG	5 cut(s)	355, 468, 481, 929, 1409
SduI	GDGCHC	2 cut(s)	258, 1179
SfaNI	GCATC	7 cut(s)	375, 781, 909, 1649, 1822, 1863, 2032
SfoI	GGCGCC	1 cut(s)	289
SmiMI	CAYNNNNRTG	2 cut(s)	723, 1268
SmlI	CTYRAG	4 cut(s)	164, 1438, 1628, 1838
SmoI	CTYRAG	4 cut(s)	164, 1438, 1628, 1838
SsiI	CCGC	1 cut(s)	687
SspDI	GGCGCC	1 cut(s)	287
SspI	AATATT	4 cut(s)	19, 1039, 1485, 1792
SspMI	CTAG	1 cut(s)	2197
StyD4I	CCNGG	5 cut(s)	353, 466, 479, 927, 1407
StyI	CCWWGG	2 cut(s)	865, 1359
TaaI	ACNGT	5 cut(s)	68, 1086, 1494, 1535, 1570
TaiI	ACGT	3 cut(s)	641, 1472, 1586
TaqI	TCGA	4 cut(s)	249, 263, 1275, 1668
TatI	WGTACW	2 cut(s)	321, 830
TfiI	GAWTC	8 cut(s)	160, 683, 889, 1168, 1858, 1945, 2146, 2177
TscAI	CASTG	5 cut(s)	71, 744, 1225, 1573, 2024
TseFI	GTSAC	3 cut(s)	195, 949, 1885
TseI	GCWGC	5 cut(s)	350, 484, 899, 1134, 1239
Tsp45I	GTSAC	3 cut(s)	195, 949, 1885
TspRI	CASTG	5 cut(s)	71, 744, 1225, 1573, 2024
Tth111I	GACNNNGTC	2 cut(s)	1347, 1883
Vha464I	CTTAAG	2 cut(s)	164, 1628
VspI	ATTAAT	3 cut(s)	1062, 1590, 1800
XapI	RAATTY	3 cut(s)	40, 359, 733
XceI	RCATGY	2 cut(s)	607, 764
XspI	CTAG	1 cut(s)	2197
Zsp2I	ATGCAT	2 cut(s)	796, 972

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.