Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0007312

Physical Location & Seq

Genomic Coordinates Forward (+)

23832064 .. 23836658

Gene Structure 4595 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00236510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2148 bp

ATGTTAAAAATTCCAAAACTTTGGCATCCTCTGACCAAAAAGTTGCAATCTTTGATCAATACTCACCGTTTTGGGATTTTCTCAGCTGGGTATGTTAAGGGTAGCAATGACAATGGTTTAGAGTCAGAGAATGAGTGGGAGAGGTTGCTTAAGCCATTTGACCTCAATGAACTTCGGAAATCACTCGTTCAAATTACCCCAGTTCAGCTTTGTAAGTTACTTGAACTCCCACTTGACGTGCCCACGTCAATGGAAATATTCGAATTGGTGGGTGCCCAGAGGGGATATAGCCATTCATTTGATGTGTACTATATGTTGATGGATAAGCTGGGCGCGGCGGGGGAGTTTAAGGTCATAGATAGGTTGTTGATGCAGATTAAAGATGAAGGGATGGTTTTTAGAGAGTCCATTTTCATTATGATCATGAAACATTATGGCAGAGCGGGCTTGCCTGGTCAGGCGACTAGGCTGCTTCTGGATATGAGAGGGGTTTATTCTTTTGAACCTACTTTCAAATCTTACAATGCTGTTTTGGATATACTGGTGGCTGGGAATTGCCCCAAGGTGGCACCCAATGTGTTCTATGACATGTTGAGTAGGGGTATCTCTCCCAATGTTTACACATTTGGGCTGGTTCTGAAGGCATTTTGTATGGTTAATGAGGTGGATTCTGCATGTTCGCTTCTTAGAGACATGACGAAGCATGGGTGTGTGCCGAATTCTGTGGTTTACCAGACTCTTATACATGCCCTCTCCAGAAATGAGCGAGTGAATGAAGCATTGAGACTTTTGGAGGAAATGTTCTTGATGGCTTGCACTCCTGATGTTCAGACTTTTAATGATATTATCCATGGTCTCTGCAAGGCCAATCGGATTCAGGAGGCAGCAAAGTTGGTTGATCGGATGCTTTTGCGGGGTTTTACTCCTGATGAGATAACTTATGGAATTTTAATGAACGGATTATGTAGAACAGGGCAAGTTGATGAAGCAAAGGCATTGTTAAATAAAGTGCCTAGCCCGAATGCTGTGCTTTTCAATACCTTGATTAATGGTTATGTTACCAGTGGACGCTTTGATGAAGCCAAAGTTGTCTACTGTGATGGCATGCTGGGTAACAGCTGTGGCCTTGATGTTTATACATTTAACATTCTGATTCGTGGACTTTGCAAGAAAGGCAGTTTGGCTTCTGCTCGTGAATTGGTCAGTGAGATGGAAACTAAGGGCTGCAAGCCCAATGTGATAACTTACACGATATTGGTCGATGGGTTATGCAAGGAAGGTCAACTGCAGGAAGCTGGTGATGTTTTAAATGAGATGTCATACAAGGGTCTCGGTCCGAATATCGTGGGATACAATTGTCTAATATCTGCTCTCTGCAAGGATGGAAAGGTCCACGAAGCTTTAAAGTTGTTTGGTGAAATCTCAAGCAAAGGATGTAAGGCAGACATATTTACCTTTAATTCTTTGATATTTGGATTGTGCAAGGTTGATAGGATGGAAGAGGCATTGGGATTGTACCATGATATGTTATTGGAGGGTGTTATTGCCAACACTGTAACTTATAACACATTAATTCATGGTTTTCTGACTGGAGGTGCGATCCAAGAAGCACTGAAGCTTGTAAATGAAATGGTGTCTAGAGGATGCCCTCTTGACAAAATAACCTATAATGGGTTAGTAAAGGCACTCTGCAGAGCTGGGGCTGTTGAAAAAGCAAGGGGATTGTTTGAAGAAATGATGAGGACAGGACTTCATCCAGATAGTATCTCATGCAATATTTTGATTAATGGCCTCTGTAAAAGTGGCAAAGTATATGAAGCCCTTGAGTTCCTAAGGGATATGATTCATCGCGGTTTAATGCCCGATATAGTCACTTATAATAGCCTGATAAATGGTATGTGCAAGATGGGGCACATTTGGGAAGCTTTGAATCTCTTTGACAGATTACAAGCTGAAGGGGTATGCCCTGATGCTATTACTTATAACACTTTGATCAGTTGGCATTGCAAAGAGGGCATGATTAATGATGCTTGTTTGCTTCTACATAGAGGTGTGACTAATGGATTCATACCAAATCATGTGACGTGGTATATATTGGTCAGCAACCTGTCTAAAGAAAGAGGTTATGTCCATCAGGCCTTTATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0019538 GO:0032543 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:0140053 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

715

Amino Acids

79.7

Weight (kDa)

6.64

Isoelectric Point (pI)

23.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	173 - 217	2.2e-08	PPR repeat family
TPR_24	PF23276	185 - 298	9.7e-09	Fungal tetratrico peptide repeats
PPR_1	PF12854	200 - 233	1.4e-06	PPR repeat
PPR_2	PF13041	212 - 252	1.2e-08	PPR repeat family
PPR_3	PF13812	227 - 284	1.6e-06	Pentatricopeptide repeat domain
PPR_long	PF17177	229 - 337	2.4e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	236 - 267	2.8e-09	PPR repeat
PPR_2	PF13041	239 - 288	1.8e-13	PPR repeat family
PPR_1	PF12854	272 - 302	8.4e-10	PPR repeat
PPR_2	PF13041	274 - 323	7e-16	PPR repeat family
PPR_1	PF12854	305 - 336	7.6e-10	PPR repeat
PPR_2	PF13041	340 - 390	1.3e-12	PPR repeat family
PPR_1	PF12854	374 - 405	2.3e-10	PPR repeat
PPR_3	PF13812	376 - 420	4.5e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	377 - 425	9.8e-19	PPR repeat family
PPR	PF01535	379 - 409	1.8e-06	PPR repeat
TPR_24	PF23276	403 - 508	1.3e-07	Fungal tetratrico peptide repeats
PPR_1	PF12854	407 - 440	1.2e-13	PPR repeat
PPR	PF01535	414 - 444	1.7e-06	PPR repeat
PPR_3	PF13812	435 - 490	1.6e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	442 - 473	4.8e-11	PPR repeat
PPR_2	PF13041	446 - 495	8.2e-18	PPR repeat family
PPR	PF01535	451 - 479	4.4e-08	PPR repeat
PPR_long	PF17177	459 - 615	1.3e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	477 - 509	3.8e-09	PPR repeat
PPR_2	PF13041	482 - 528	6.7e-14	PPR repeat family
PPR	PF01535	484 - 514	1.1e-06	PPR repeat
PPR_1	PF12854	513 - 545	7.7e-07	PPR repeat
TPR_24	PF23276	513 - 612	1e-05	Fungal tetratrico peptide repeats
PPR_2	PF13041	517 - 565	1.4e-16	PPR repeat family
PPR	PF01535	519 - 549	2e-06	PPR repeat
TPR_24	PF23276	545 - 648	1.8e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	547 - 579	4.7e-10	PPR repeat
PPR	PF01535	554 - 583	1.6e-06	PPR repeat
PPR_2	PF13041	554 - 600	4.4e-14	PPR repeat family
PPR_1	PF12854	583 - 614	2.4e-09	PPR repeat
PPR_2	PF13041	586 - 635	6.6e-18	PPR repeat family
PPR	PF01535	589 - 619	2.4e-07	PPR repeat
PPR_3	PF13812	611 - 667	6.8e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	617 - 648	1e-12	PPR repeat
PPR_2	PF13041	621 - 665	1.7e-14	PPR repeat family
PPR	PF01535	624 - 654	8.6e-08	PPR repeat
PPR_1	PF12854	653 - 682	1.2e-09	PPR repeat
PPR_2	PF13041	656 - 705	2.9e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007312)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64320
fragaria_vesca	FvH4_5g29651 FvH4_5g29651
malus_domestica	MD02G1307900.v1.1
prunus_persica	Prupe.4G007800_v2.0.a1 Prupe.4G007800_v2.0.a1 Prupe.4G007800_v2.0.a1 Prupe.4G007800_v2.0.a1 Prupe.4G007800_v2.0.a1 Prupe.4G007800_v2.0.a1 Prupe.4G007800_v2.0.a1
pyrus_communis	pycom02g25910
rosa_chinensis	RchiOBHm_Chr7g0222741
rosa_laevigata	RLG00000002027
rosa_multiflora	Rmu_sc0007870.1_g000004 Rmu_sc0025009.1_g000002
rosa_roxburghii	Rroxscaffold_3G00236510
rosa_rugosa	Rorug07G0212100 Rorug07G0212200 Rorug07G0212200
rosa_samantha	Rh7AG356400 Rh7BG344800 Rh7CG373900 Rh7DG350700
rosa_wichuraiana	Rw7G030240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1563, 1878, 1983
AccB1I	GGYRCC	2 cut(s)	272, 568
AccBSI	CCGCTC	1 cut(s)	443
AccI	GTMKAC	1 cut(s)	1092
AccII	CGCG	2 cut(s)	335, 1851
AciI	CCGC	5 cut(s)	335, 338, 443, 913, 1851
AclWI	GGATC	1 cut(s)	1594
AcsI	RAATTY	3 cut(s)	9, 718, 945
AcuI	CTGAAG	3 cut(s)	659, 1634, 1974
AfaI	GTAC	2 cut(s)	308, 1517
AfiI	CCNNNNNNNGG	2 cut(s)	565, 1671
AflII	CTTAAG	1 cut(s)	149
AflIII	ACRYGT	1 cut(s)	588
AgsI	TTSAA	8 cut(s)	191, 224, 503, 514, 1036, 1709, 1730, 1930
AjiI	CACGTC	3 cut(s)	238, 246, 2085
AjnI	CCWGG	1 cut(s)	451
AjuI	GAANNNNNNNTTGG	2 cut(s)	1163, 1195
AloI	GAACNNNNNNTCC	2 cut(s)	785, 817
Alw26I	GTCTC	4 cut(s)	684, 778, 860, 1334
AlwI	GGATC	1 cut(s)	1594
AoxI	GGCC	4 cut(s)	864, 1123, 1789, 2136
ApeKI	GCWGC	3 cut(s)	469, 884, 1224
ApoI	RAATTY	3 cut(s)	9, 718, 945
AseI	ATTAAT	4 cut(s)	1047, 1571, 1785, 2022
AspLEI	GCGC	1 cut(s)	335
AspS9I	GGNCC	2 cut(s)	1334, 1390
AsuHPI	GGTGA	3 cut(s)	56, 1310, 1427
AsuII	TTCGAA	1 cut(s)	261
AvaII	GGWCC	2 cut(s)	1334, 1390
AxyI	CCTNAGG	1 cut(s)	1832
BaeGI	GKGCMC	3 cut(s)	243, 277, 1914
BaeI	ACNNNNGTAYC	2 cut(s)	1342, 1375
BanI	GGYRCC	2 cut(s)	272, 568
BauI	CACGAG	1 cut(s)	1191
BbvI	GCAGC	3 cut(s)	456, 896, 1211
BcgI	CGANNNNNNTGC	4 cut(s)	451, 485, 1009, 1043
BciT130I	CCWGG	1 cut(s)	453
BciVI	GTATCC	1 cut(s)	1343
BclI	TGATCA	3 cut(s)	54, 420, 1992
BcoDI	GTCTC	4 cut(s)	684, 778, 860, 1334
BfaI	CTAG	3 cut(s)	465, 1014, 1638
BfmI	CTRYAG	2 cut(s)	1286, 1690
BfrI	CTTAAG	1 cut(s)	149
BfuI	GTATCC	1 cut(s)	1343
BisI	GCNGC	4 cut(s)	336, 470, 885, 1225
BlsI	GCNGC	4 cut(s)	337, 471, 886, 1226
Bme1390I	CCNGG	1 cut(s)	453
Bme18I	GGWCC	2 cut(s)	1334, 1390
BmgBI	CACGTC	3 cut(s)	238, 246, 2085
BmgT120I	GGNCC	2 cut(s)	1334, 1390
BmiI	GGNNCC	2 cut(s)	274, 570
BmrFI	CCNGG	1 cut(s)	453
BmrI	ACTGGG	1 cut(s)	194
BmsI	GCATC	6 cut(s)	34, 360, 894, 1634, 1960, 2017
BmuI	ACTGGG	1 cut(s)	194
BpmI	CTGGAG	2 cut(s)	739, 1611
Bpu14I	TTCGAA	1 cut(s)	261
BpuEI	CTTGAG	2 cut(s)	1408, 1844
BsaI	GGTCTC	2 cut(s)	860, 1334
BsaJI	CCNNGG	2 cut(s)	561, 850
BsaXI	ACNNNNNCTCC	4 cut(s)	210, 240, 785, 815
Bsc4I	CCNNNNNNNGG	2 cut(s)	565, 1671
Bse1I	ACTGG	4 cut(s)	200, 546, 1062, 1594
Bse21I	CCTNAGG	1 cut(s)	1832
Bse3DI	GCAATG	2 cut(s)	112, 2002
BseBI	CCWGG	1 cut(s)	453
BseDI	CCNNGG	2 cut(s)	561, 850
BseGI	GGATG	8 cut(s)	25, 396, 909, 1387, 1439, 1500, 1649, 1753
BseLI	CCNNNNNNNGG	2 cut(s)	565, 1671
BseMI	GCAATG	2 cut(s)	112, 2002
BseMII	CTCAG	1 cut(s)	96
BseNI	ACTGG	4 cut(s)	200, 546, 1062, 1594
BseSI	GKGCMC	3 cut(s)	243, 277, 1914
BseXI	GCAGC	3 cut(s)	456, 896, 1211
BseYI	CCCAGC	5 cut(s)	86, 328, 548, 1108, 1697
Bsh1236I	CGCG	2 cut(s)	335, 1851
BshFI	GGCC	4 cut(s)	866, 1125, 1791, 2138
BshNI	GGYRCC	2 cut(s)	272, 568
BslI	CCNNNNNNNGG	2 cut(s)	565, 1671
BsmAI	GTCTC	4 cut(s)	684, 778, 860, 1334
BsmI	GAATGC	1 cut(s)	1027
BsnI	GGCC	4 cut(s)	866, 1125, 1791, 2138
Bso31I	GGTCTC	2 cut(s)	860, 1334
Bsp119I	TTCGAA	1 cut(s)	261
Bsp1286I	GDGCHC	3 cut(s)	243, 277, 1914
Bsp143I	GATC	5 cut(s)	54, 420, 898, 1599, 1992
Bsp19I	CCATGG	1 cut(s)	850
BspACI	CCGC	5 cut(s)	335, 338, 443, 913, 1851
BspANI	GGCC	4 cut(s)	866, 1125, 1791, 2138
BspCNI	CTCAG	1 cut(s)	95
BspFNI	CGCG	2 cut(s)	335, 1851
BspHI	TCATGA	1 cut(s)	423
BspLI	GGNNCC	2 cut(s)	274, 570
BspMAI	CTGCAG	2 cut(s)	1290, 1694
BspPI	GGATC	1 cut(s)	1594
BspT104I	TTCGAA	1 cut(s)	261
BspT107I	GGYRCC	2 cut(s)	272, 568
BspTI	CTTAAG	1 cut(s)	149
BspTNI	GGTCTC	2 cut(s)	860, 1334
BsrBI	CCGCTC	1 cut(s)	443
BsrDI	GCAATG	2 cut(s)	112, 2002
BsrI	ACTGG	4 cut(s)	200, 546, 1062, 1594
BssECI	CCNNGG	2 cut(s)	561, 850
BssMI	GATC	5 cut(s)	54, 420, 898, 1599, 1992
BssSI	CACGAG	1 cut(s)	1191
BssT1I	CCWWGG	2 cut(s)	561, 850
Bst2BI	CACGAG	1 cut(s)	1191
Bst2UI	CCWGG	1 cut(s)	453
Bst4CI	ACNGT	3 cut(s)	68, 1097, 1555
Bst6I	CTCTTC	1 cut(s)	1494
BstAFI	CTTAAG	1 cut(s)	149
BstBI	TTCGAA	1 cut(s)	261
BstC8I	GCNNGC	5 cut(s)	445, 449, 814, 1106, 1229
BstDEI	CTNAG	4 cut(s)	82, 686, 1218, 1832
BstDSI	CCRYGG	1 cut(s)	850
BstF5I	GGATG	8 cut(s)	25, 396, 909, 1387, 1439, 1500, 1649, 1753
BstFNI	CGCG	2 cut(s)	335, 1851
BstHHI	GCGC	1 cut(s)	335
BstKTI	GATC	5 cut(s)	57, 423, 901, 1602, 1995
BstMAI	GTCTC	4 cut(s)	684, 778, 860, 1334
BstMBI	GATC	5 cut(s)	54, 420, 898, 1599, 1992
BstMWI	GCNNNNNNNGC	3 cut(s)	444, 1173, 2013
BstNI	CCWGG	1 cut(s)	453
BstNSI	RCATGY	4 cut(s)	592, 678, 749, 1108
BstSCI	CCNGG	1 cut(s)	451
BstSFI	CTRYAG	2 cut(s)	1286, 1690
BstSLI	GKGCMC	3 cut(s)	243, 277, 1914
BstUI	CGCG	2 cut(s)	335, 1851
BstV1I	GCAGC	3 cut(s)	456, 896, 1211
BstXI	CCANNNNNNTGG	2 cut(s)	21, 250
Bsu36I	CCTNAGG	1 cut(s)	1832
BsuI	GTATCC	1 cut(s)	1343
BsuRI	GGCC	4 cut(s)	866, 1125, 1791, 2138
BtgI	CCRYGG	1 cut(s)	850
BtgZI	GCGATG	1 cut(s)	1832
BtrI	CACGTC	3 cut(s)	238, 246, 2085
BtsCI	GGATG	8 cut(s)	25, 396, 909, 1387, 1439, 1500, 1649, 1753
BtsIMutI	CAGTG	4 cut(s)	1069, 1210, 1551, 1610
Cac8I	GCNNGC	5 cut(s)	445, 449, 814, 1106, 1229
CciI	TCATGA	1 cut(s)	423
CfoI	GCGC	1 cut(s)	335
Cfr13I	GGNCC	2 cut(s)	1334, 1390
CpoI	CGGWCCG	1 cut(s)	1334
CseI	GACGC	1 cut(s)	1077
Csp6I	GTAC	2 cut(s)	307, 1516
CspI	CGGWCCG	1 cut(s)	1334
CviQI	GTAC	2 cut(s)	307, 1516
DdeI	CTNAG	4 cut(s)	82, 686, 1218, 1832
DpnI	GATC	5 cut(s)	56, 422, 900, 1601, 1994
DpnII	GATC	5 cut(s)	54, 420, 898, 1599, 1992
DraI	TTTAAA	2 cut(s)	1308, 1404
Eam1104I	CTCTTC	1 cut(s)	1494
EarI	CTCTTC	1 cut(s)	1494
Eco130I	CCWWGG	2 cut(s)	561, 850
Eco147I	AGGCCT	1 cut(s)	2138
Eco31I	GGTCTC	2 cut(s)	860, 1334
Eco47I	GGWCC	2 cut(s)	1334, 1390
Eco57I	CTGAAG	3 cut(s)	659, 1634, 1974
Eco81I	CCTNAGG	1 cut(s)	1832
EcoRI	GAATTC	1 cut(s)	718
EcoRII	CCWGG	1 cut(s)	451
EcoT14I	CCWWGG	2 cut(s)	561, 850
ErhI	CCWWGG	2 cut(s)	561, 850
FauI	CCCGC	3 cut(s)	331, 436, 906
FbaI	TGATCA	3 cut(s)	54, 420, 1992
FblI	GTMKAC	1 cut(s)	1092
Fnu4HI	GCNGC	4 cut(s)	336, 470, 885, 1225
FokI	GGATG	8 cut(s)	12, 403, 916, 1394, 1446, 1507, 1656, 1740
Fsp4HI	GCNGC	4 cut(s)	336, 470, 885, 1225
FspBI	CTAG	3 cut(s)	465, 1014, 1638
GlaI	GCGC	1 cut(s)	334
GluI	GCNGC	4 cut(s)	336, 470, 885, 1225
GsaI	CCCAGC	5 cut(s)	90, 332, 552, 1112, 1701
GsuI	CTGGAG	2 cut(s)	739, 1611
HaeIII	GGCC	4 cut(s)	866, 1125, 1791, 2138
HgaI	GACGC	1 cut(s)	1077
HhaI	GCGC	1 cut(s)	335
Hin6I	GCGC	1 cut(s)	333
HinP1I	GCGC	1 cut(s)	333
HincII	GTYRAC	1 cut(s)	1283
HindII	GTYRAC	1 cut(s)	1283
HindIII	AAGCTT	3 cut(s)	1398, 1616, 1923
HinfI	GANTC	9 cut(s)	122, 404, 668, 736, 874, 1153, 1843, 1930, 2064
HphI	GGTGA	3 cut(s)	56, 1310, 1427
Hpy166II	GTNNAC	8 cut(s)	307, 619, 730, 1067, 1093, 1160, 1283, 1393
Hpy8I	GTNNAC	8 cut(s)	307, 619, 730, 1067, 1093, 1160, 1283, 1393
HpyAV	CCTTC	4 cut(s)	380, 634, 1271, 1949
HpyCH4III	ACNGT	3 cut(s)	68, 1097, 1555
HpyCH4IV	ACGT	3 cut(s)	237, 245, 2084
HpyF10VI	GCNNNNNNNGC	3 cut(s)	444, 1173, 2013
HpyF3I	CTNAG	4 cut(s)	82, 686, 1218, 1832
HpySE526I	ACGT	3 cut(s)	237, 245, 2084
HspAI	GCGC	1 cut(s)	333
Ksp22I	TGATCA	3 cut(s)	54, 420, 1992
Kzo9I	GATC	5 cut(s)	54, 420, 898, 1599, 1992
Lsp1109I	GCAGC	3 cut(s)	456, 896, 1211
LweI	GCATC	6 cut(s)	34, 360, 894, 1634, 1960, 2017
MaeI	CTAG	3 cut(s)	465, 1014, 1638
MaeII	ACGT	3 cut(s)	237, 245, 2084
MaeIII	GTNAC	7 cut(s)	216, 1057, 1112, 1555, 1870, 2053, 2080
MalI	GATC	5 cut(s)	56, 422, 900, 1601, 1994
MbiI	CCGCTC	1 cut(s)	443
MboI	GATC	5 cut(s)	54, 420, 898, 1599, 1992
MboII	GAAGA	2 cut(s)	1511, 1742
MfeI	CAATTG	1 cut(s)	1354
MhlI	GDGCHC	3 cut(s)	243, 277, 1914
MlyI	GAGTC	3 cut(s)	131, 413, 730
MslI	CAYNNNNRTG	3 cut(s)	248, 708, 2049
MspA1I	CMGCKG	2 cut(s)	86, 1119
MspCI	CTTAAG	1 cut(s)	149
MspR9I	CCNGG	1 cut(s)	453
MunI	CAATTG	1 cut(s)	1354
Mva1269I	GAATGC	1 cut(s)	1027
MvaI	CCWGG	1 cut(s)	453
MvnI	CGCG	2 cut(s)	335, 1851
MwoI	GCNNNNNNNGC	3 cut(s)	444, 1173, 2013
NcoI	CCATGG	1 cut(s)	850
NdeII	GATC	5 cut(s)	54, 420, 898, 1599, 1992
NlaIV	GGNNCC	2 cut(s)	274, 570
NmuCI	GTSAC	3 cut(s)	1870, 2053, 2080
NspI	RCATGY	4 cut(s)	592, 678, 749, 1108
NspV	TTCGAA	1 cut(s)	261
PaeI	GCATGC	1 cut(s)	1108
PagI	TCATGA	1 cut(s)	423
PceI	AGGCCT	1 cut(s)	2138
PciI	ACATGT	1 cut(s)	588
PctI	GAATGC	1 cut(s)	1027
PfeI	GAWTC	6 cut(s)	668, 874, 1153, 1843, 1930, 2064
PkrI	GCNGC	4 cut(s)	337, 471, 886, 1226
PleI	GAGTC	3 cut(s)	130, 412, 730
PpsI	GAGTC	3 cut(s)	130, 412, 730
PscI	ACATGT	1 cut(s)	588
PshBI	ATTAAT	4 cut(s)	1047, 1571, 1785, 2022
PsiI	TTATAA	3 cut(s)	1563, 1878, 1983
Psp6I	CCWGG	1 cut(s)	451
PspFI	CCCAGC	5 cut(s)	86, 328, 548, 1108, 1697
PspGI	CCWGG	1 cut(s)	451
PspN4I	GGNNCC	2 cut(s)	274, 570
PspPI	GGNCC	2 cut(s)	1334, 1390
PstI	CTGCAG	2 cut(s)	1290, 1694
PvuII	CAGCTG	2 cut(s)	86, 1119
RsaI	GTAC	2 cut(s)	308, 1517
RsaNI	GTAC	2 cut(s)	307, 1516
RseI	CAYNNNNRTG	3 cut(s)	248, 708, 2049
Rsr2I	CGGWCCG	1 cut(s)	1334
RsrII	CGGWCCG	1 cut(s)	1334
SatI	GCNGC	4 cut(s)	336, 470, 885, 1225
Sau3AI	GATC	5 cut(s)	54, 420, 898, 1599, 1992
Sau96I	GGNCC	2 cut(s)	1334, 1390
SchI	GAGTC	3 cut(s)	131, 413, 730
ScrFI	CCNGG	1 cut(s)	453
SduI	GDGCHC	3 cut(s)	243, 277, 1914
SfaNI	GCATC	6 cut(s)	34, 360, 894, 1634, 1960, 2017
SfcI	CTRYAG	2 cut(s)	1286, 1690
SfuI	TTCGAA	1 cut(s)	261
SinI	GGWCC	2 cut(s)	1334, 1390
SmiMI	CAYNNNNRTG	3 cut(s)	248, 708, 2049
SmlI	CTYRAG	3 cut(s)	149, 1423, 1823
SmoI	CTYRAG	3 cut(s)	149, 1423, 1823
SphI	GCATGC	1 cut(s)	1108
SseBI	AGGCCT	1 cut(s)	2138
SsiI	CCGC	5 cut(s)	335, 338, 443, 913, 1851
SspI	AATATT	2 cut(s)	258, 1777
SspMI	CTAG	3 cut(s)	465, 1014, 1638
StuI	AGGCCT	1 cut(s)	2138
StyD4I	CCNGG	1 cut(s)	451
StyI	CCWWGG	2 cut(s)	561, 850
TaaI	ACNGT	3 cut(s)	68, 1097, 1555
TaiI	ACGT	3 cut(s)	240, 248, 2087
TaqI	TCGA	2 cut(s)	261, 1260
TaqII	GACCGA	1 cut(s)	1322
TatI	WGTACW	1 cut(s)	306
TauI	GCSGC	1 cut(s)	338
TfiI	GAWTC	6 cut(s)	668, 874, 1153, 1843, 1930, 2064
TscAI	CASTG	4 cut(s)	1069, 1210, 1558, 1617
TseFI	GTSAC	3 cut(s)	1870, 2053, 2080
TseI	GCWGC	3 cut(s)	469, 884, 1224
Tsp45I	GTSAC	3 cut(s)	1870, 2053, 2080
TspGWI	ACGGA	1 cut(s)	972
TspRI	CASTG	4 cut(s)	1069, 1210, 1558, 1617
Vha464I	CTTAAG	1 cut(s)	149
VpaK11BI	GGWCC	2 cut(s)	1334, 1390
VspI	ATTAAT	4 cut(s)	1047, 1571, 1785, 2022
XapI	RAATTY	3 cut(s)	9, 718, 945
XbaI	TCTAGA	1 cut(s)	1637
XceI	RCATGY	4 cut(s)	592, 678, 749, 1108
XmiI	GTMKAC	1 cut(s)	1092
XspI	CTAG	3 cut(s)	465, 1014, 1638

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.