pycom03g00640

acyl-activating enzyme 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005780
Physical Location & Seq
Forward (+)
527778 .. 547766
19989 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1629 bp
ATGCAAATCCCTGCAAAACCACAGAGAGAGAGAGAGAGAGAGAGAGAAATGGTGAAGAACATAAGTGAGATGGGAGTGGAGGACTTTGTTCAGGCAGGGCTAGACGTTGAAGAGGCCAAGGATTTCGGAACAGTCCTAAAAAATACAATCTCCCGCACGAACACAACGGACCCAAAAGAGATATGGCGGCAGATAGTGGCTGAGAGATTGCTGAAACCATCACACCCTCATGAGCTGCACCAGTTGATCTACTACTCAGTCTATGCCAATTGGGATGCCTCCACCAAAGGCCCTCCTCTCTACTGGTTCCCTTCTCTCAGCACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATGGTGAAGAACATAAGTGAGGTGGGAGTGGAGGACTTCGTTCAGGCAGGGCTAGACGTTGAAGAGGCCAAGGATTTCGAAACAGTCCTCAAAAATACAATCTCCCGCACGAACACAACGGACCCAAAAGAGATATGGCGGCAGATAGTCGCTGAGAGATTGCTGAAACCATCACACCCTCATGAGCTGCACCAGTCGATCTACTACTCAGTCTATGCCAATTGGGATGCCTCCACCAAAGGCCCTCCTTTCTACTGGTTCCCTTCTCTATACCAGTCGCGACAAACAAACTTGGGGCGATTGATGGAAAAGCACGGTCCAGAGCTTTTAGGAACATCATATAAGGATCCCATAACAAGTTACACACTCTTCCAGGAATTCTCTGTTCAGAATCCCGAGGTTTACTGGTCACTTGTTCTAAAAGAGCTCTCGGTTTCCTTTCGAAAAGTCCCAAAGTGTATTTTGGATAGGACTGACAAATCAAAACGCGGTGGATCTTGGCTTCCACATTCAGTTTTGAATATTGCTGAATGTTGTTTGCTACCCACCAGACACCCAAGAAAAGAGGATGATGGTTTGGCTGTTGTATGGAGGGATGAAGGCTGCGACGATGATTCTTCTGTTAATCGTATGACATTAAAGGAACTTCGGGAACAAGTAATGTTGGTGGCTAATGCACTGGATGCAACCTTTTCAAAGGGTGATGCAATTGCTATCGACATGCCAATGAATGTCAATTCGGTTATCATATATTTAGCAATTGTACTAGCAGGATTTGTTGTTGTATCAATAGCAGACAGTTTTGCTGTAAATGAAATTGCAACTCGTCTACGCGTGTCTAAAGCAAAGGGTGTCTTTACCCAGGATTTCATACTAAGAGGAGGCCGAAAGTTTCCTCTCTACAGTCGAGTTGCAGAGGCCGCTCCACATTTAGCTATTGTACTTCCAGCAATTGGGAGCAATGTAGGCATTCAGTTAAGAGAACAGGATCTATCCTGGCATGATTTTCTTTCTCGTGTCGATCACCTTCCAAGAAAGAACTACTACTCTCCAGTCTATCAGCCAATCGACTCTGTGACTAATATTCTCTTTTCATCTGGAACCACAGGATCGATCCGAAAGCTATTCCATGGACACAACTTTCACCAATTAGAGCTGTTGCTGATTCATGGGCTCACAATGATAATCAAGTTGGGGATGTCTTCTGCTGGCCAACAAATTTGGGATGGGTTATGGGTCCAATCTTACTCTACTCGTGCTTTCTGA

Protein Analysis

543

Amino Acids

62.0

Weight (kDa)

6.49

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 312 - 491 2.8e-17 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1316
AccBSI CCGCTC 1 cut(s) 1286
AccI GTMKAC 1 cut(s) 1192
AccII CGCG 3 cut(s) 643, 852, 1197
AciI CCGC 6 cut(s) 154, 187, 469, 502, 852, 1284
AclWI GGATC 6 cut(s) 704, 717, 865, 1359, 1471, 1480
AcoI YGGCCR 1 cut(s) 1573
AcsI RAATTY 2 cut(s) 740, 1581
AfaI GTAC 2 cut(s) 1128, 1305
AfiI CCNNNNNNNGG 1 cut(s) 1316
AflIII ACRYGT 1 cut(s) 1195
AgsI TTSAA 4 cut(s) 110, 425, 883, 1059
AjnI CCWGG 3 cut(s) 735, 1224, 1358
AluBI AGCT 7 cut(s) 235, 550, 688, 790, 1298, 1486, 1519
AluI AGCT 7 cut(s) 235, 550, 688, 790, 1298, 1486, 1519
Alw21I GWGCWC 1 cut(s) 792
AlwI GGATC 6 cut(s) 704, 717, 865, 1359, 1471, 1480
Ama87I CYCGRG 1 cut(s) 758
AoxI GGCC 7 cut(s) 114, 289, 429, 604, 1246, 1281, 1573
ApeKI GCWGC 3 cut(s) 235, 550, 966
ApoI RAATTY 2 cut(s) 740, 1581
AspS9I GGNCC 6 cut(s) 169, 290, 484, 605, 680, 1600
AsuHPI GGTGA 5 cut(s) 64, 379, 1076, 1379, 1499
AsuII TTCGAA 2 cut(s) 441, 805
AvaI CYCGRG 1 cut(s) 758
AvaII GGWCC 4 cut(s) 169, 484, 680, 1600
BalI TGGCCA 1 cut(s) 1575
BamHI GGATCC 1 cut(s) 709
BanII GRGCYC 2 cut(s) 792, 1539
BauI CACGAG 2 cut(s) 1377, 1617
BbsI GAAGAC 1 cut(s) 1557
Bbv12I GWGCWC 1 cut(s) 792
BbvI GCAGC 3 cut(s) 222, 537, 953
BccI CCATC 7 cut(s) 64, 226, 358, 541, 661, 929, 1583
BciT130I CCWGG 3 cut(s) 737, 1226, 1360
BfaI CTAG 3 cut(s) 101, 416, 1130
BfmI CTRYAG 1 cut(s) 1264
BisI GCNGC 6 cut(s) 188, 236, 503, 551, 967, 1284
BlsI GCNGC 6 cut(s) 189, 237, 504, 552, 968, 1285
Bme1390I CCNGG 3 cut(s) 737, 1226, 1360
Bme18I GGWCC 4 cut(s) 169, 484, 680, 1600
BmeT110I CYCGRG 1 cut(s) 758
BmgT120I GGNCC 6 cut(s) 169, 290, 484, 605, 680, 1600
BmiI GGNNCC 7 cut(s) 171, 308, 486, 623, 711, 1465, 1601
BmrFI CCNGG 3 cut(s) 737, 1226, 1360
BmsI GCATC 4 cut(s) 265, 580, 1036, 1057
BpiI GAAGAC 1 cut(s) 1557
BpmI CTGGAG 1 cut(s) 1398
Bpu14I TTCGAA 2 cut(s) 441, 805
Bsa29I ATCGAT 1 cut(s) 1475
BsaJI CCNNGG 5 cut(s) 117, 432, 759, 1224, 1492
Bsc4I CCNNNNNNNGG 1 cut(s) 1316
Bse1I ACTGG 8 cut(s) 241, 308, 556, 623, 637, 773, 1047, 1415
Bse3DI GCAATG 1 cut(s) 1330
BseBI CCWGG 3 cut(s) 737, 1226, 1360
BseCI ATCGAT 1 cut(s) 1475
BseDI CCNNGG 5 cut(s) 117, 432, 759, 1224, 1492
BseGI GGATG 7 cut(s) 280, 595, 937, 964, 1051, 1566, 1594
BseLI CCNNNNNNNGG 1 cut(s) 1316
BseMI GCAATG 1 cut(s) 1330
BseMII CTCAG 5 cut(s) 192, 270, 331, 507, 585
BseNI ACTGG 8 cut(s) 241, 308, 556, 623, 637, 773, 1047, 1415
BseRI GAGGAG 2 cut(s) 285, 1257
BseXI GCAGC 3 cut(s) 222, 537, 953
BsgI GTGCAG 2 cut(s) 221, 536
Bsh1236I CGCG 3 cut(s) 643, 852, 1197
BshFI GGCC 7 cut(s) 116, 291, 431, 606, 1248, 1283, 1575
BshVI ATCGAT 1 cut(s) 1475
BsiHKAI GWGCWC 1 cut(s) 792
BsiHKCI CYCGRG 1 cut(s) 758
BslFI GGGAC 1 cut(s) 797
BslI CCNNNNNNNGG 1 cut(s) 1316
BsmFI GGGAC 1 cut(s) 797
BsmI GAATGC 1 cut(s) 1332
BsnI GGCC 7 cut(s) 116, 291, 431, 606, 1248, 1283, 1575
BsoBI CYCGRG 1 cut(s) 758
Bsp119I TTCGAA 2 cut(s) 441, 805
Bsp1286I GDGCHC 2 cut(s) 792, 1539
Bsp143I GATC 8 cut(s) 246, 561, 709, 857, 1351, 1384, 1472, 1476
Bsp19I CCATGG 1 cut(s) 1492
Bsp68I TCGCGA 1 cut(s) 643
BspACI CCGC 6 cut(s) 154, 187, 469, 502, 852, 1284
BspANI GGCC 7 cut(s) 116, 291, 431, 606, 1248, 1283, 1575
BspCNI CTCAG 5 cut(s) 193, 269, 330, 508, 584
BspDI ATCGAT 1 cut(s) 1475
BspFNI CGCG 3 cut(s) 643, 852, 1197
BspHI TCATGA 2 cut(s) 229, 544
BspLI GGNNCC 7 cut(s) 171, 308, 486, 623, 711, 1465, 1601
BspPI GGATC 6 cut(s) 704, 717, 865, 1359, 1471, 1480
BspT104I TTCGAA 2 cut(s) 441, 805
BsrBI CCGCTC 1 cut(s) 1286
BsrDI GCAATG 1 cut(s) 1330
BsrI ACTGG 8 cut(s) 241, 308, 556, 623, 637, 773, 1047, 1415
BssECI CCNNGG 5 cut(s) 117, 432, 759, 1224, 1492
BssMI GATC 8 cut(s) 246, 561, 709, 857, 1351, 1384, 1472, 1476
BssSI CACGAG 2 cut(s) 1377, 1617
BssT1I CCWWGG 3 cut(s) 117, 432, 1492
Bst2BI CACGAG 2 cut(s) 1377, 1617
Bst2UI CCWGG 3 cut(s) 737, 1226, 1360
Bst4CI ACNGT 5 cut(s) 133, 448, 680, 1163, 1268
Bst6I CTCTTC 3 cut(s) 105, 420, 737
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstBI TTCGAA 2 cut(s) 441, 805
BstC8I GCNNGC 1 cut(s) 1573
BstDEI CTNAG 6 cut(s) 201, 256, 317, 516, 571, 1238
BstDSI CCRYGG 1 cut(s) 1492
BstF5I GGATG 7 cut(s) 280, 595, 937, 964, 1051, 1566, 1594
BstFNI CGCG 3 cut(s) 643, 852, 1197
BstKTI GATC 8 cut(s) 249, 564, 712, 860, 1354, 1387, 1475, 1479
BstMBI GATC 8 cut(s) 246, 561, 709, 857, 1351, 1384, 1472, 1476
BstMWI GCNNNNNNNGC 3 cut(s) 1046, 1283, 1329
BstNI CCWGG 3 cut(s) 737, 1226, 1360
BstNSI RCATGY 1 cut(s) 1087
BstSCI CCNGG 3 cut(s) 735, 1224, 1358
BstSFI CTRYAG 1 cut(s) 1264
BstUI CGCG 3 cut(s) 643, 852, 1197
BstV1I GCAGC 3 cut(s) 222, 537, 953
BstV2I GAAGAC 1 cut(s) 1557
BstX2I RGATCY 3 cut(s) 709, 857, 1351
BstYI RGATCY 3 cut(s) 709, 857, 1351
Bsu15I ATCGAT 1 cut(s) 1475
BsuRI GGCC 7 cut(s) 116, 291, 431, 606, 1248, 1283, 1575
BsuTUI ATCGAT 1 cut(s) 1475
BtgI CCRYGG 1 cut(s) 1492
BtsCI GGATG 7 cut(s) 280, 595, 937, 964, 1051, 1566, 1594
BtsIMutI CAGTG 1 cut(s) 1040
BtuMI TCGCGA 1 cut(s) 643
Cac8I GCNNGC 1 cut(s) 1573
CciI TCATGA 2 cut(s) 229, 544
Cfr13I GGNCC 6 cut(s) 169, 290, 484, 605, 680, 1600
ClaI ATCGAT 1 cut(s) 1475
Csp6I GTAC 2 cut(s) 1127, 1304
CviAII CATG 6 cut(s) 230, 545, 1084, 1364, 1493, 1532
CviQI GTAC 2 cut(s) 1127, 1304
DdeI CTNAG 6 cut(s) 201, 256, 317, 516, 571, 1238
DpnI GATC 8 cut(s) 248, 563, 711, 859, 1353, 1386, 1474, 1478
DpnII GATC 8 cut(s) 246, 561, 709, 857, 1351, 1384, 1472, 1476
EaeI YGGCCR 1 cut(s) 1573
Eam1104I CTCTTC 3 cut(s) 105, 420, 737
EarI CTCTTC 3 cut(s) 105, 420, 737
Ecl136II GAGCTC 1 cut(s) 790
Eco130I CCWWGG 3 cut(s) 117, 432, 1492
Eco24I GRGCYC 2 cut(s) 792, 1539
Eco47I GGWCC 4 cut(s) 169, 484, 680, 1600
Eco53kI GAGCTC 1 cut(s) 790
Eco88I CYCGRG 1 cut(s) 758
EcoICRI GAGCTC 1 cut(s) 790
EcoO109I RGGNCCY 2 cut(s) 290, 605
EcoRI GAATTC 1 cut(s) 740
EcoRII CCWGG 3 cut(s) 735, 1224, 1358
EcoT14I CCWWGG 3 cut(s) 117, 432, 1492
EcoT38I GRGCYC 2 cut(s) 792, 1539
ErhI CCWWGG 3 cut(s) 117, 432, 1492
FaeI CATG 6 cut(s) 233, 548, 1087, 1367, 1496, 1535
FalI AAGNNNNNCTT 2 cut(s) 1202, 1234
FaqI GGGAC 1 cut(s) 797
FatI CATG 6 cut(s) 229, 544, 1083, 1363, 1492, 1531
FauI CCCGC 2 cut(s) 161, 476
FblI GTMKAC 1 cut(s) 1192
Fnu4HI GCNGC 6 cut(s) 188, 236, 503, 551, 967, 1284
FokI GGATG 7 cut(s) 287, 602, 944, 971, 1058, 1573, 1601
FriOI GRGCYC 2 cut(s) 792, 1539
Fsp4HI GCNGC 6 cut(s) 188, 236, 503, 551, 967, 1284
FspBI CTAG 3 cut(s) 101, 416, 1130
GluI GCNGC 6 cut(s) 188, 236, 503, 551, 967, 1284
GsuI CTGGAG 1 cut(s) 1398
HaeIII GGCC 7 cut(s) 116, 291, 431, 606, 1248, 1283, 1575
Hin1II CATG 6 cut(s) 233, 548, 1087, 1367, 1496, 1535
HinfI GANTC 4 cut(s) 754, 977, 1433, 1528
HphI GGTGA 5 cut(s) 64, 379, 1076, 1379, 1499
Hpy166II GTNNAC 2 cut(s) 766, 1193
Hpy188I TCNGA 4 cut(s) 128, 753, 1481, 1628
Hpy188III TCNNGA 7 cut(s) 230, 545, 642, 683, 758, 1013, 1461
Hpy8I GTNNAC 2 cut(s) 766, 1193
Hpy99I CGWCG 1 cut(s) 974
HpyAV CCTTC 4 cut(s) 321, 636, 956, 1400
HpyCH4III ACNGT 5 cut(s) 133, 448, 680, 1163, 1268
HpyCH4IV ACGT 2 cut(s) 105, 420
HpyCH4V TGCA 9 cut(s) 4, 14, 238, 553, 1040, 1049, 1070, 1184, 1277
HpyF10VI GCNNNNNNNGC 3 cut(s) 1046, 1283, 1329
HpyF3I CTNAG 6 cut(s) 201, 256, 317, 516, 571, 1238
HpySE526I ACGT 2 cut(s) 105, 420
Hsp92II CATG 6 cut(s) 233, 548, 1087, 1367, 1496, 1535
Kzo9I GATC 8 cut(s) 246, 561, 709, 857, 1351, 1384, 1472, 1476
LmnI GCTCC 2 cut(s) 1291, 1320
Lsp1109I GCAGC 3 cut(s) 222, 537, 953
LweI GCATC 4 cut(s) 265, 580, 1036, 1057
MaeI CTAG 3 cut(s) 101, 416, 1130
MaeII ACGT 2 cut(s) 105, 420
MaeIII GTNAC 3 cut(s) 722, 771, 1438
MalI GATC 8 cut(s) 248, 563, 711, 859, 1353, 1386, 1474, 1478
MbiI CCGCTC 1 cut(s) 1286
MboI GATC 8 cut(s) 246, 561, 709, 857, 1351, 1384, 1472, 1476
MboII GAAGA 7 cut(s) 67, 122, 382, 437, 724, 972, 1557
MfeI CAATTG 5 cut(s) 268, 583, 1071, 1122, 1314
MflI RGATCY 3 cut(s) 709, 857, 1351
MhlI GDGCHC 2 cut(s) 792, 1539
MlsI TGGCCA 1 cut(s) 1575
MluI ACGCGT 1 cut(s) 1195
MluNI TGGCCA 1 cut(s) 1575
MlyI GAGTC 1 cut(s) 1427
Mox20I TGGCCA 1 cut(s) 1575
MscI TGGCCA 1 cut(s) 1575
MseI TTAA 3 cut(s) 987, 1001, 1340
MslI CAYNNNNRTG 3 cut(s) 228, 543, 1088
Msp20I TGGCCA 1 cut(s) 1575
MspR9I CCNGG 3 cut(s) 737, 1226, 1360
MunI CAATTG 5 cut(s) 268, 583, 1071, 1122, 1314
Mva1269I GAATGC 1 cut(s) 1332
MvaI CCWGG 3 cut(s) 737, 1226, 1360
MvnI CGCG 3 cut(s) 643, 852, 1197
MwoI GCNNNNNNNGC 3 cut(s) 1046, 1283, 1329
NcoI CCATGG 1 cut(s) 1492
NdeII GATC 8 cut(s) 246, 561, 709, 857, 1351, 1384, 1472, 1476
NlaIII CATG 6 cut(s) 233, 548, 1087, 1367, 1496, 1535
NlaIV GGNNCC 7 cut(s) 171, 308, 486, 623, 711, 1465, 1601
NmuCI GTSAC 2 cut(s) 771, 1438
NruI TCGCGA 1 cut(s) 643
NspI RCATGY 1 cut(s) 1087
NspV TTCGAA 2 cut(s) 441, 805
PagI TCATGA 2 cut(s) 229, 544
PctI GAATGC 1 cut(s) 1332
PfeI GAWTC 3 cut(s) 754, 977, 1528
PflMI CCANNNNNTGG 1 cut(s) 1316
PfoI TCCNGGA 1 cut(s) 735
PkrI GCNGC 6 cut(s) 189, 237, 504, 552, 968, 1285
PleI GAGTC 1 cut(s) 1427
PpsI GAGTC 1 cut(s) 1427
Psp124BI GAGCTC 1 cut(s) 792
Psp6I CCWGG 3 cut(s) 735, 1224, 1358
PspGI CCWGG 3 cut(s) 735, 1224, 1358
PspN4I GGNNCC 7 cut(s) 171, 308, 486, 623, 711, 1465, 1601
PspPI GGNCC 6 cut(s) 169, 290, 484, 605, 680, 1600
PsuI RGATCY 3 cut(s) 709, 857, 1351
RruI TCGCGA 1 cut(s) 643
RsaI GTAC 2 cut(s) 1128, 1305
RsaNI GTAC 2 cut(s) 1127, 1304
RseI CAYNNNNRTG 3 cut(s) 228, 543, 1088
SacI GAGCTC 1 cut(s) 792
SaqAI TTAA 3 cut(s) 987, 1001, 1340
SatI GCNGC 6 cut(s) 188, 236, 503, 551, 967, 1284
Sau3AI GATC 8 cut(s) 246, 561, 709, 857, 1351, 1384, 1472, 1476
Sau96I GGNCC 6 cut(s) 169, 290, 484, 605, 680, 1600
SchI GAGTC 1 cut(s) 1427
ScrFI CCNGG 3 cut(s) 737, 1226, 1360
SduI GDGCHC 2 cut(s) 792, 1539
SfaNI GCATC 4 cut(s) 265, 580, 1036, 1057
SfcI CTRYAG 1 cut(s) 1264
SfuI TTCGAA 2 cut(s) 441, 805
SinI GGWCC 4 cut(s) 169, 484, 680, 1600
SmiMI CAYNNNNRTG 3 cut(s) 228, 543, 1088
SsiI CCGC 6 cut(s) 154, 187, 469, 502, 852, 1284
SspI AATATT 2 cut(s) 886, 1447
SspMI CTAG 3 cut(s) 101, 416, 1130
SstI GAGCTC 1 cut(s) 792
StyD4I CCNGG 3 cut(s) 735, 1224, 1358
StyI CCWWGG 3 cut(s) 117, 432, 1492
TaaI ACNGT 5 cut(s) 133, 448, 680, 1163, 1268
TaiI ACGT 2 cut(s) 108, 423
TaqI TCGA 8 cut(s) 441, 560, 805, 1080, 1270, 1383, 1431, 1475
TatI WGTACW 2 cut(s) 1126, 1303
TauI GCSGC 3 cut(s) 190, 505, 1286
TfiI GAWTC 3 cut(s) 754, 977, 1528
Tru1I TTAA 3 cut(s) 987, 1001, 1340
Tru9I TTAA 3 cut(s) 987, 1001, 1340
TscAI CASTG 1 cut(s) 1047
TseFI GTSAC 2 cut(s) 771, 1438
TseI GCWGC 3 cut(s) 235, 550, 966
Tsp45I GTSAC 2 cut(s) 771, 1438
TspDTI ATGAA 6 cut(s) 975, 1106, 1191, 1222, 1446, 1520
TspGWI ACGGA 2 cut(s) 182, 497
TspRI CASTG 1 cut(s) 1047
Van91I CCANNNNNTGG 1 cut(s) 1316
VpaK11BI GGWCC 4 cut(s) 169, 484, 680, 1600
XapI RAATTY 2 cut(s) 740, 1581
XceI RCATGY 1 cut(s) 1087
XcmI CCANNNNNNNNNTGG 2 cut(s) 180, 495
XmiI GTMKAC 1 cut(s) 1192
XspI CTAG 3 cut(s) 101, 416, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.