pycom03g17060

ubiE/COQ5 methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009982
Physical Location & Seq
Forward (+)
18189761 .. 18190678
918 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g17060.1

Sequence Viewer

Length: 918 bp
ATGCCTTCTGGAAACATGTTCAGAGAGAGGAAGATGGGGAAACCATCAGGTGCGGCACCAGGCAGCAGAGACTGGACTCAGATCTACGCAATCTACGGTCTCGACCAGCTGCACACCATTCTCTTCCTTCTCTTCAACGCTGTCCTATTTACCGTTCTGTCACTCCTCTACCTCACTTATTTCAACCCCATCACCCTCTTCTTCCACCGCCTCCTCCCTATCGACACCGCCCGTTTTGCCGCCGGGTTCACTGGCTCTGTCACCGCCCTTTCCGCCCTCTGTCTCTTCTTCGCATCCGGACACTGCCTATACTCCTCACTCTCCCTCCACTACGACGTCGCCCAGCGCATGGTCGGCTCAGTCAACGAATGGTCGACCGTCAAGCACGCCCTGGACCTCGGCTGCGGCCGCGGCATACTTTTAAACGCGGTGGCGACCCAGATGAAGAAAGAGGGGAGTTCGGGTCGGGTTGTGGGCCTGGACCGGTCCAAGATGACGAGCCTCTCGTCTACGCTGCGGGCAGCGAAGATTGAAGGTGTGGGGGAGTACGTGACTTGCCGCGAGGGCGACCCCAGGAGATTGCCGTTTGGGGACGGGTACTTCGACGTCGTGGTGTCTGCGGTGTTCGTCCACACCGTCGGAAAAGAGTACGGCCATCGGACGGTGGAGGCGTCGGCGGAGCGGATGAGGGTTGTCGGCGAGATGGTGAGGGTTCTGAAGCCCGGTGGGGTGGGGGTGGTGTGGGACCTACTGCACGTGCCGGAGTACGTGCGGCGGCTGCAAGAGCTGAAGATGGAGGACATTCGGGTGTCGGAGCGGGTAACGGCGTTTATGGCGAGCAGCCAGATCGTGTCGTTTCGCAAGCCCAGTCAGCACGTTATTGGGCCCGGTGAGGTCCGACTCGACTGGAGATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

306

Amino Acids

33.84

Weight (kDa)

9.31

Isoelectric Point (pI)

30.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 117 - 249 1.7e-06 ubiE/COQ5 methyltransferase family
Methyltransf_31 PF13847 129 - 251 2.7e-11 Methyltransferase domain
Methyltransf_11 PF08241 131 - 245 1.1e-14 Methyltransferase domain
Methyltransf_25 PF13649 131 - 243 4.9e-12 Methyltransferase domain
Methyltransf_12 PF08242 131 - 244 1.5e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009982)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15530 AT3G15530 AT5G54400
fragaria_vesca FvH4_3g20760 FvH4_3g20760
malus_domestica MD03G1221800.v1.1 MD11G1239200.v1.1
prunus_persica Prupe.4G187700_v2.0.a1 Prupe.4G187700_v2.0.a1
pyrus_communis pycom03g17060 pycom11g21090
rosa_chinensis RchiOBHm_Chr5g0034991
rosa_laevigata RLG00000033590
rosa_multiflora Rmu_sc0000879.1_g000037
rosa_roxburghii Rroxscaffold_1G00045400
rosa_rugosa Rorug05G0149300
rosa_samantha Rh5AG242300 Rh5BG241900 Rh5CG273400 Rh5DG250500
rosa_wichuraiana Rw5G022130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 339, 609
AccB1I GGYRCC 1 cut(s) 55
AccB7I CCANNNNNTGG 1 cut(s) 349
AccBSI CCGCTC 2 cut(s) 682, 817
AccI GTMKAC 2 cut(s) 374, 509
AccII CGCG 3 cut(s) 411, 428, 561
AccIII TCCGGA 1 cut(s) 296
AcoI YGGCCR 2 cut(s) 406, 652
AcuI CTGAAG 2 cut(s) 737, 809
AcvI CACGTG 1 cut(s) 757
AcyI GRCGYC 3 cut(s) 336, 606, 671
AfaI GTAC 4 cut(s) 548, 599, 650, 767
AfiI CCNNNNNNNGG 2 cut(s) 349, 661
AflIII ACRYGT 1 cut(s) 15
AgeI ACCGGT 1 cut(s) 483
AgsI TTSAA 3 cut(s) 136, 184, 533
AjnI CCWGG 4 cut(s) 58, 390, 477, 572
AluBI AGCT 2 cut(s) 109, 787
AluI AGCT 2 cut(s) 109, 787
Alw26I GTCTC 3 cut(s) 63, 104, 287
AlwNI CAGNNNCTG 1 cut(s) 72
Aor13HI TCCGGA 1 cut(s) 296
AoxI GGCC 4 cut(s) 406, 475, 652, 884
ApaI GGGCCC 1 cut(s) 888
ApeKI GCWGC 7 cut(s) 63, 109, 402, 514, 521, 778, 840
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
AsiGI ACCGGT 1 cut(s) 483
AspLEI GCGC 1 cut(s) 348
AspS9I GGNCC 8 cut(s) 394, 475, 481, 486, 745, 884, 885, 895
AsuC2I CCSGG 3 cut(s) 244, 723, 888
AsuHPI GGTGA 4 cut(s) 184, 253, 718, 902
AvaII GGWCC 5 cut(s) 394, 481, 486, 745, 895
BaeGI GKGCMC 1 cut(s) 888
BanI GGYRCC 1 cut(s) 55
BanII GRGCYC 1 cut(s) 888
BbrPI CACGTG 1 cut(s) 757
BbvI GCAGC 7 cut(s) 75, 96, 389, 501, 533, 765, 852
BccI CCATC 6 cut(s) 28, 52, 197, 663, 697, 787
BceAI ACGGC 3 cut(s) 568, 667, 840
BcgI CGANNNNNNTGC 2 cut(s) 829, 863
BciT130I CCWGG 4 cut(s) 60, 392, 479, 574
BcnI CCSGG 3 cut(s) 244, 723, 888
BcoDI GTCTC 3 cut(s) 63, 104, 287
BglI GCCNNNNNGGC 1 cut(s) 564
BglII AGATCT 1 cut(s) 81
Bme1390I CCNGG 7 cut(s) 60, 244, 392, 479, 574, 723, 888
Bme18I GGWCC 5 cut(s) 394, 481, 486, 745, 895
BmgT120I GGNCC 8 cut(s) 394, 475, 481, 486, 745, 884, 885, 895
BmiI GGNNCC 3 cut(s) 57, 746, 886
BmrFI CCNGG 7 cut(s) 60, 244, 392, 479, 574, 723, 888
BmrI ACTGGG 1 cut(s) 861
BmsI GCATC 2 cut(s) 302, 902
BmuI ACTGGG 1 cut(s) 861
BpuMI CCSGG 3 cut(s) 244, 723, 888
BsaAI YACGTR 3 cut(s) 550, 757, 769
BsaHI GRCGYC 3 cut(s) 336, 606, 671
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 4 cut(s) 390, 397, 409, 572
BsaWI WCCGGW 2 cut(s) 296, 483
BsaXI ACNNNNNCTCC 4 cut(s) 309, 339, 448, 478
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 661
Bse118I RCCGGY 1 cut(s) 483
Bse1I ACTGG 4 cut(s) 77, 256, 867, 911
BseAI TCCGGA 1 cut(s) 296
BseBI CCWGG 4 cut(s) 60, 392, 479, 574
BseDI CCNNGG 4 cut(s) 390, 397, 409, 572
BseGI GGATG 2 cut(s) 293, 690
BseLI CCNNNNNNNGG 2 cut(s) 349, 661
BseMII CTCAG 2 cut(s) 92, 372
BseNI ACTGG 4 cut(s) 77, 256, 867, 911
BseRI GAGGAG 3 cut(s) 155, 203, 304
BseSI GKGCMC 1 cut(s) 888
BseX3I CGGCCG 1 cut(s) 406
BseXI GCAGC 7 cut(s) 75, 96, 389, 501, 533, 765, 852
BseYI CCCAGC 1 cut(s) 342
BsgI GTGCAG 2 cut(s) 95, 737
Bsh1236I CGCG 3 cut(s) 411, 428, 561
Bsh1285I CGRYCG 2 cut(s) 378, 409
BshFI GGCC 4 cut(s) 408, 477, 654, 886
BshNI GGYRCC 1 cut(s) 55
BshTI ACCGGT 1 cut(s) 483
BsiEI CGRYCG 2 cut(s) 378, 409
BsiSI CCGG 6 cut(s) 243, 297, 484, 723, 761, 888
BslFI GGGAC 2 cut(s) 605, 758
BslI CCNNNNNNNGG 2 cut(s) 349, 661
BsmAI GTCTC 3 cut(s) 63, 104, 287
BsmFI GGGAC 2 cut(s) 605, 758
BsnI GGCC 4 cut(s) 408, 477, 654, 886
Bso31I GGTCTC 1 cut(s) 104
Bsp120I GGGCCC 1 cut(s) 884
Bsp1286I GDGCHC 1 cut(s) 888
Bsp13I TCCGGA 1 cut(s) 296
Bsp143I GATC 2 cut(s) 81, 846
BspANI GGCC 4 cut(s) 408, 477, 654, 886
BspCNI CTCAG 2 cut(s) 91, 371
BspEI TCCGGA 1 cut(s) 296
BspFNI CGCG 3 cut(s) 411, 428, 561
BspLI GGNNCC 3 cut(s) 57, 746, 886
BspT107I GGYRCC 1 cut(s) 55
BspTNI GGTCTC 1 cut(s) 104
BsrBI CCGCTC 2 cut(s) 682, 817
BsrFI RCCGGY 1 cut(s) 483
BsrI ACTGG 4 cut(s) 77, 256, 867, 911
BssAI RCCGGY 1 cut(s) 483
BssECI CCNNGG 4 cut(s) 390, 397, 409, 572
BssMI GATC 2 cut(s) 81, 846
BssNI GRCGYC 3 cut(s) 336, 606, 671
Bst2UI CCWGG 4 cut(s) 60, 392, 479, 574
Bst4CI ACNGT 5 cut(s) 98, 154, 379, 637, 664
Bst6I CTCTTC 4 cut(s) 128, 137, 203, 290
BstACI GRCGYC 3 cut(s) 336, 606, 671
BstBAI YACGTR 3 cut(s) 550, 757, 769
BstC8I GCNNGC 4 cut(s) 387, 519, 838, 863
BstDEI CTNAG 2 cut(s) 78, 358
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 2 cut(s) 293, 690
BstFNI CGCG 3 cut(s) 411, 428, 561
BstHHI GCGC 1 cut(s) 348
BstKTI GATC 2 cut(s) 84, 849
BstMAI GTCTC 3 cut(s) 63, 104, 287
BstMBI GATC 2 cut(s) 81, 846
BstMCI CGRYCG 2 cut(s) 378, 409
BstNI CCWGG 4 cut(s) 60, 392, 479, 574
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 7 cut(s) 58, 242, 390, 477, 572, 721, 886
BstSLI GKGCMC 1 cut(s) 888
BstUI CGCG 3 cut(s) 411, 428, 561
BstV1I GCAGC 7 cut(s) 75, 96, 389, 501, 533, 765, 852
BstX2I RGATCY 1 cut(s) 81
BstYI RGATCY 1 cut(s) 81
BstZI CGGCCG 1 cut(s) 406
BsuRI GGCC 4 cut(s) 408, 477, 654, 886
BtgI CCRYGG 1 cut(s) 409
BtsCI GGATG 2 cut(s) 293, 690
BtsI GCAGTG 1 cut(s) 301
BtsIMutI CAGTG 2 cut(s) 249, 301
Cac8I GCNNGC 4 cut(s) 387, 519, 838, 863
CaiI CAGNNNCTG 1 cut(s) 72
CciNI GCGGCCGC 1 cut(s) 406
CfoI GCGC 1 cut(s) 348
Cfr10I RCCGGY 1 cut(s) 483
Cfr13I GGNCC 8 cut(s) 394, 475, 481, 486, 745, 884, 885, 895
Cfr42I CCGCGG 1 cut(s) 412
CseI GACGC 1 cut(s) 660
Csp6I GTAC 4 cut(s) 547, 598, 649, 766
CspAI ACCGGT 1 cut(s) 483
CviAII CATG 2 cut(s) 16, 349
CviQI GTAC 4 cut(s) 547, 598, 649, 766
DdeI CTNAG 2 cut(s) 78, 358
DpnI GATC 2 cut(s) 83, 848
DpnII GATC 2 cut(s) 81, 846
DraI TTTAAA 1 cut(s) 423
EaeI YGGCCR 2 cut(s) 406, 652
EagI CGGCCG 1 cut(s) 406
Eam1104I CTCTTC 4 cut(s) 128, 137, 203, 290
EarI CTCTTC 4 cut(s) 128, 137, 203, 290
EciI GGCGGA 2 cut(s) 262, 692
EclXI CGGCCG 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 888
Eco31I GGTCTC 1 cut(s) 104
Eco47I GGWCC 5 cut(s) 394, 481, 486, 745, 895
Eco52I CGGCCG 1 cut(s) 406
Eco57I CTGAAG 2 cut(s) 737, 809
Eco72I CACGTG 1 cut(s) 757
EcoO109I RGGNCCY 1 cut(s) 745
EcoRII CCWGG 4 cut(s) 58, 390, 477, 572
EcoT38I GRGCYC 1 cut(s) 888
FaeI CATG 2 cut(s) 19, 352
FaiI YATR 5 cut(s) 17, 310, 350, 416, 833
FaqI GGGAC 2 cut(s) 605, 758
FatI CATG 2 cut(s) 15, 348
FauI CCCGC 2 cut(s) 510, 810
FblI GTMKAC 2 cut(s) 374, 509
FokI GGATG 2 cut(s) 280, 697
FriOI GRGCYC 1 cut(s) 888
GlaI GCGC 1 cut(s) 347
GsaI CCCAGC 1 cut(s) 346
HaeIII GGCC 4 cut(s) 408, 477, 654, 886
HapII CCGG 6 cut(s) 243, 297, 484, 723, 761, 888
HgaI GACGC 1 cut(s) 660
HhaI GCGC 1 cut(s) 348
Hin1I GRCGYC 3 cut(s) 336, 606, 671
Hin1II CATG 2 cut(s) 19, 352
Hin6I GCGC 1 cut(s) 346
HinP1I GCGC 1 cut(s) 346
HincII GTYRAC 2 cut(s) 364, 375
HindII GTYRAC 2 cut(s) 364, 375
HinfI GANTC 2 cut(s) 76, 900
HpaII CCGG 6 cut(s) 243, 297, 484, 723, 761, 888
HphI GGTGA 4 cut(s) 184, 253, 718, 902
Hpy166II GTNNAC 5 cut(s) 249, 364, 375, 510, 631
Hpy188I TCNGA 7 cut(s) 23, 81, 641, 660, 717, 814, 899
Hpy188III TCNNGA 3 cut(s) 9, 101, 297
Hpy8I GTNNAC 5 cut(s) 249, 364, 375, 510, 631
Hpy99I CGWCG 6 cut(s) 338, 341, 608, 611, 641, 676
HpyAV CCTTC 3 cut(s) 15, 137, 527
HpyCH4III ACNGT 5 cut(s) 98, 154, 379, 637, 664
HpyCH4IV ACGT 6 cut(s) 336, 549, 606, 756, 768, 876
HpyCH4V TGCA 3 cut(s) 112, 754, 781
HpyF3I CTNAG 2 cut(s) 78, 358
HpySE526I ACGT 6 cut(s) 336, 549, 606, 756, 768, 876
Hsp92I GRCGYC 3 cut(s) 336, 606, 671
Hsp92II CATG 2 cut(s) 19, 352
HspAI GCGC 1 cut(s) 346
Kpn2I TCCGGA 1 cut(s) 296
KspI CCGCGG 1 cut(s) 412
Kzo9I GATC 2 cut(s) 81, 846
LmnI GCTCC 2 cut(s) 679, 814
Lsp1109I GCAGC 7 cut(s) 75, 96, 389, 501, 533, 765, 852
LweI GCATC 2 cut(s) 302, 902
MaeII ACGT 6 cut(s) 336, 549, 606, 756, 768, 876
MaeIII GTNAC 4 cut(s) 159, 259, 550, 820
MalI GATC 2 cut(s) 83, 848
MbiI CCGCTC 2 cut(s) 682, 817
MboI GATC 2 cut(s) 81, 846
MflI RGATCY 1 cut(s) 81
MhlI GDGCHC 1 cut(s) 888
MlyI GAGTC 2 cut(s) 70, 894
MmeI TCCRAC 2 cut(s) 619, 792
MroI TCCGGA 1 cut(s) 296
MseI TTAA 1 cut(s) 422
MslI CAYNNNNRTG 1 cut(s) 806
MspA1I CMGCKG 2 cut(s) 109, 411
MspI CCGG 6 cut(s) 243, 297, 484, 723, 761, 888
MspR9I CCNGG 7 cut(s) 60, 244, 392, 479, 574, 723, 888
MvaI CCWGG 4 cut(s) 60, 392, 479, 574
MvnI CGCG 3 cut(s) 411, 428, 561
NciI CCSGG 3 cut(s) 244, 723, 888
NdeII GATC 2 cut(s) 81, 846
NlaIII CATG 2 cut(s) 19, 352
NlaIV GGNNCC 3 cut(s) 57, 746, 886
NmeAIII GCCGAG 1 cut(s) 378
NmuCI GTSAC 3 cut(s) 159, 259, 550
NotI GCGGCCGC 1 cut(s) 406
NspI RCATGY 1 cut(s) 19
PciI ACATGT 1 cut(s) 15
PcsI WCGNNNNNNNCGW 4 cut(s) 503, 600, 633, 668
PflMI CCANNNNNTGG 1 cut(s) 349
PinAI ACCGGT 1 cut(s) 483
PleI GAGTC 2 cut(s) 70, 894
PmaCI CACGTG 1 cut(s) 757
PmlI CACGTG 1 cut(s) 757
PpsI GAGTC 2 cut(s) 70, 894
Ppu21I YACGTR 3 cut(s) 550, 757, 769
PpuMI RGGWCCY 1 cut(s) 745
PscI ACATGT 1 cut(s) 15
Psp5II RGGWCCY 1 cut(s) 745
Psp6I CCWGG 4 cut(s) 58, 390, 477, 572
PspCI CACGTG 1 cut(s) 757
PspFI CCCAGC 1 cut(s) 342
PspGI CCWGG 4 cut(s) 58, 390, 477, 572
PspN4I GGNNCC 3 cut(s) 57, 746, 886
PspOMI GGGCCC 1 cut(s) 884
PspPI GGNCC 8 cut(s) 394, 475, 481, 486, 745, 884, 885, 895
PspPPI RGGWCCY 1 cut(s) 745
PstNI CAGNNNCTG 1 cut(s) 72
PsuI RGATCY 1 cut(s) 81
PvuII CAGCTG 1 cut(s) 109
RsaI GTAC 4 cut(s) 548, 599, 650, 767
RsaNI GTAC 4 cut(s) 547, 598, 649, 766
RseI CAYNNNNRTG 1 cut(s) 806
SacII CCGCGG 1 cut(s) 412
SalI GTCGAC 1 cut(s) 373
SaqAI TTAA 1 cut(s) 422
Sau3AI GATC 2 cut(s) 81, 846
Sau96I GGNCC 8 cut(s) 394, 475, 481, 486, 745, 884, 885, 895
SchI GAGTC 2 cut(s) 70, 894
ScrFI CCNGG 7 cut(s) 60, 244, 392, 479, 574, 723, 888
SduI GDGCHC 1 cut(s) 888
SfaNI GCATC 2 cut(s) 302, 902
Sfr303I CCGCGG 1 cut(s) 412
SgrBI CCGCGG 1 cut(s) 412
SinI GGWCC 5 cut(s) 394, 481, 486, 745, 895
SmiMI CAYNNNNRTG 1 cut(s) 806
StyD4I CCNGG 7 cut(s) 58, 242, 390, 477, 572, 721, 886
TaaI ACNGT 5 cut(s) 98, 154, 379, 637, 664
TaiI ACGT 6 cut(s) 339, 552, 609, 759, 771, 879
TaqI TCGA 5 cut(s) 102, 222, 374, 603, 903
TauI GCSGC 8 cut(s) 56, 242, 408, 411, 414, 561, 775, 778
Tru1I TTAA 1 cut(s) 422
Tru9I TTAA 1 cut(s) 422
TscAI CASTG 2 cut(s) 256, 308
TseFI GTSAC 3 cut(s) 159, 259, 550
TseI GCWGC 7 cut(s) 63, 109, 402, 514, 521, 778, 840
Tsp45I GTSAC 3 cut(s) 159, 259, 550
TspDTI ATGAA 1 cut(s) 458
TspRI CASTG 2 cut(s) 256, 308
Van91I CCANNNNNTGG 1 cut(s) 349
VpaK11BI GGWCC 5 cut(s) 394, 481, 486, 745, 895
XceI RCATGY 1 cut(s) 19
XmiI GTMKAC 2 cut(s) 374, 509
ZraI GACGTC 2 cut(s) 337, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.