Rh5CG273400

ubiE/COQ5 methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0009982
Physical Location & Seq
Reverse (-)
29552943 .. 29555252
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG273400.1

Sequence Viewer

Length: 867 bp
ATGGGGAAATCAGGCAACAGAGACTGGACCCAGATCTACGCCATCTACGGGCTCGACCAGTGGCAGACCATACTGTTCCTACTCTTCCACGCCGTGCTATTTACCATTTTGTCCCTCCTCTACCTCACATATTTCGACCCCATCACCCTCTTCTTCCACCGCCTCCTCCCCATCGGCTCCGCCCGCTTTGCCGCGGGATTCACCGGCTCCGTCACCGCCCTCTCCGCCCTCTGCCTCTTCTTCGCCTCCTTCCACTTCCTCCACTCCTCCCTCCCCCTCCACTACGACGTCGTCCAGCGCATGGTCGCCTCCGTCAACGACTGGTCAACCGTCAAGCACGCCCTCGACCTCGGCTGCGGCCGCGGCATCCTCCTCAACGCCGTCGCCACCCAGCTCAAGAAGGAGGGCAGTTCGGGCCGGGTCGTCGGGTTGGACCGGTCCAAGATGACGAGCCTTTCCACCTTGCGGACCGCCAAGCTGGAAGGTGTCGGGGAGTACGTGACCTGCCGTGAGGGCGACCCCAGGAGATTGCCGTTCGGGGACAACTACTTCGACGTGGTGGTATCCGCTGTGTTCGTCCACACCGTAGGGAAGGAGTACGGGCATCGGACGGTGGAGGCTTCGGCCGAGAGGATGAGGGTGGTCGGTGAGATGGTCCGGGTCTTAAAGCCGGGTGGGTCCGGGGTGGTGTGGGACCTACTGCACGTTCCCGAATACGTGCGGAGGCTGCAGGAGCTGAAGATGGAGGACATTCGGGTCTCGGAGCGGGTCACGGCCTTCATGGTCAGCAGTCAAATCGTGTCCTTTCGGAAGCCCAGTCACCACTATTTTGGGCTCGGAGAGGTCAGGCTGGACTGGAGATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

288

Amino Acids

32.44

Weight (kDa)

9.17

Isoelectric Point (pI)

27.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 100 - 232 1.1e-06 ubiE/COQ5 methyltransferase family
Methyltransf_31 PF13847 113 - 234 4.3e-11 Methyltransferase domain
Methyltransf_11 PF08241 115 - 227 4e-15 Methyltransferase domain
Methyltransf_25 PF13649 115 - 226 1.7e-12 Methyltransferase domain
Methyltransf_12 PF08242 115 - 226 1.5e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009982)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15530 AT3G15530 AT5G54400
fragaria_vesca FvH4_3g20760 FvH4_3g20760
malus_domestica MD03G1221800.v1.1 MD11G1239200.v1.1
prunus_persica Prupe.4G187700_v2.0.a1 Prupe.4G187700_v2.0.a1
pyrus_communis pycom03g17060 pycom11g21090
rosa_chinensis RchiOBHm_Chr5g0034991
rosa_laevigata RLG00000033590
rosa_multiflora Rmu_sc0000879.1_g000037
rosa_roxburghii Rroxscaffold_1G00045400
rosa_rugosa Rorug05G0149300
rosa_samantha Rh5AG242300 Rh5BG241900 Rh5CG273400 Rh5DG250500
rosa_wichuraiana Rw5G022130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 755
AatII GACGTC 1 cut(s) 291
Acc36I ACCTGC 1 cut(s) 512
AccB7I CCANNNNNTGG 1 cut(s) 301
AccBSI CCGCTC 1 cut(s) 766
AccII CGCG 2 cut(s) 194, 363
AcoI YGGCCR 2 cut(s) 358, 624
AcuI CTGAAG 1 cut(s) 758
AcyI GRCGYC 1 cut(s) 288
AdeI CACNNNGTG 1 cut(s) 94
AfaI GTAC 2 cut(s) 497, 599
AfiI CCNNNNNNNGG 3 cut(s) 48, 301, 465
AgeI ACCGGT 1 cut(s) 435
AjiI CACGTC 1 cut(s) 556
AjnI CCWGG 1 cut(s) 521
AluBI AGCT 3 cut(s) 394, 478, 736
AluI AGCT 3 cut(s) 394, 478, 736
Alw26I GTCTC 2 cut(s) 15, 763
AlwNI CAGNNNCTG 2 cut(s) 24, 736
AoxI GGCC 4 cut(s) 358, 415, 624, 774
ApeKI GCWGC 2 cut(s) 354, 727
ArsI GACNNNNNNTTYG 2 cut(s) 533, 565
AsiGI ACCGGT 1 cut(s) 435
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 8 cut(s) 27, 415, 433, 438, 468, 655, 678, 694
AsuC2I CCSGG 4 cut(s) 419, 659, 672, 682
AsuHPI GGTGA 5 cut(s) 136, 193, 205, 659, 812
AvaII GGWCC 7 cut(s) 27, 433, 438, 468, 655, 678, 694
BanII GRGCYC 2 cut(s) 54, 837
BbvI GCAGC 2 cut(s) 341, 714
BccI CCATC 5 cut(s) 50, 149, 179, 646, 736
BceAI ACGGC 5 cut(s) 77, 365, 492, 517, 789
BcgI CGANNNNNNTGC 2 cut(s) 778, 812
BciT130I CCWGG 1 cut(s) 523
BciVI GTATCC 1 cut(s) 574
BcnI CCSGG 4 cut(s) 419, 659, 672, 682
BcoDI GTCTC 2 cut(s) 15, 763
BfmI CTRYAG 1 cut(s) 728
BfuAI ACCTGC 1 cut(s) 512
BfuI GTATCC 1 cut(s) 574
BglI GCCNNNNNGGC 1 cut(s) 513
BglII AGATCT 1 cut(s) 33
BisI GCNGC 6 cut(s) 192, 355, 358, 361, 364, 728
BlsI GCNGC 6 cut(s) 193, 356, 359, 362, 365, 729
Bme1390I CCNGG 5 cut(s) 419, 523, 659, 672, 682
Bme18I GGWCC 7 cut(s) 27, 433, 438, 468, 655, 678, 694
BmgBI CACGTC 1 cut(s) 556
BmgT120I GGNCC 8 cut(s) 27, 415, 433, 438, 468, 655, 678, 694
BmiI GGNNCC 5 cut(s) 29, 178, 208, 679, 695
BmrFI CCNGG 5 cut(s) 419, 523, 659, 672, 682
BmrI ACTGGG 1 cut(s) 810
BmsI GCATC 3 cut(s) 375, 613, 851
BmuI ACTGGG 1 cut(s) 810
BpuEI CTTGAG 1 cut(s) 380
BpuMI CCSGG 4 cut(s) 419, 659, 672, 682
BsaAI YACGTR 2 cut(s) 499, 718
BsaHI GRCGYC 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 763
BsaJI CCNNGG 5 cut(s) 192, 349, 361, 521, 681
BsaWI WCCGGW 1 cut(s) 435
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 301, 465
Bse118I RCCGGY 2 cut(s) 203, 435
Bse1I ACTGG 5 cut(s) 29, 58, 326, 816, 860
BseBI CCWGG 1 cut(s) 523
BseDI CCNNGG 5 cut(s) 192, 349, 361, 521, 681
BseGI GGATG 2 cut(s) 366, 639
BseLI CCNNNNNNNGG 3 cut(s) 48, 301, 465
BseNI ACTGG 5 cut(s) 29, 58, 326, 816, 860
BseRI GAGGAG 4 cut(s) 107, 155, 256, 362
BseX3I CGGCCG 2 cut(s) 358, 624
BseXI GCAGC 2 cut(s) 341, 714
BseYI CCCAGC 1 cut(s) 390
BsgI GTGCAG 1 cut(s) 686
Bsh1236I CGCG 2 cut(s) 194, 363
Bsh1285I CGRYCG 2 cut(s) 361, 627
BshFI GGCC 4 cut(s) 360, 417, 626, 776
BshTI ACCGGT 1 cut(s) 435
BsiEI CGRYCG 2 cut(s) 361, 627
BsiSI CCGG 6 cut(s) 204, 418, 436, 658, 671, 681
BslFI GGGAC 3 cut(s) 97, 554, 707
BslI CCNNNNNNNGG 3 cut(s) 48, 301, 465
BsmAI GTCTC 2 cut(s) 15, 763
BsmFI GGGAC 3 cut(s) 97, 554, 707
BsnI GGCC 4 cut(s) 360, 417, 626, 776
Bso31I GGTCTC 1 cut(s) 763
Bsp1286I GDGCHC 2 cut(s) 54, 837
Bsp143I GATC 1 cut(s) 33
BspANI GGCC 4 cut(s) 360, 417, 626, 776
BspFNI CGCG 2 cut(s) 194, 363
BspLI GGNNCC 5 cut(s) 29, 178, 208, 679, 695
BspMAI CTGCAG 1 cut(s) 732
BspMI ACCTGC 1 cut(s) 512
BspTNI GGTCTC 1 cut(s) 763
BsrBI CCGCTC 1 cut(s) 766
BsrFI RCCGGY 2 cut(s) 203, 435
BsrI ACTGG 5 cut(s) 29, 58, 326, 816, 860
BssAI RCCGGY 2 cut(s) 203, 435
BssECI CCNNGG 5 cut(s) 192, 349, 361, 521, 681
BssMI GATC 1 cut(s) 33
BssNI GRCGYC 1 cut(s) 288
Bst2UI CCWGG 1 cut(s) 523
Bst4CI ACNGT 4 cut(s) 75, 331, 586, 613
Bst6I CTCTTC 3 cut(s) 89, 155, 242
BstACI GRCGYC 1 cut(s) 288
BstBAI YACGTR 2 cut(s) 499, 718
BstC8I GCNNGC 2 cut(s) 184, 339
BstDSI CCRYGG 2 cut(s) 192, 361
BstF5I GGATG 2 cut(s) 366, 639
BstFNI CGCG 2 cut(s) 194, 363
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 1 cut(s) 36
BstMAI GTCTC 2 cut(s) 15, 763
BstMBI GATC 1 cut(s) 33
BstMCI CGRYCG 2 cut(s) 361, 627
BstMWI GCNNNNNNNGC 9 cut(s) 183, 188, 224, 360, 363, 414, 513, 727, 733
BstNI CCWGG 1 cut(s) 523
BstSCI CCNGG 5 cut(s) 417, 521, 657, 670, 680
BstSFI CTRYAG 1 cut(s) 728
BstUI CGCG 2 cut(s) 194, 363
BstV1I GCAGC 2 cut(s) 341, 714
BstX2I RGATCY 1 cut(s) 33
BstXI CCANNNNNNTGG 1 cut(s) 830
BstYI RGATCY 1 cut(s) 33
BstZI CGGCCG 2 cut(s) 358, 624
BsuI GTATCC 1 cut(s) 574
BsuRI GGCC 4 cut(s) 360, 417, 626, 776
BtgI CCRYGG 2 cut(s) 192, 361
BtrI CACGTC 1 cut(s) 556
BtsCI GGATG 2 cut(s) 366, 639
BtsIMutI CAGTG 1 cut(s) 65
BveI ACCTGC 1 cut(s) 512
Cac8I GCNNGC 2 cut(s) 184, 339
CaiI CAGNNNCTG 2 cut(s) 24, 736
CciNI GCGGCCGC 1 cut(s) 358
CfoI GCGC 1 cut(s) 300
Cfr10I RCCGGY 2 cut(s) 203, 435
Cfr13I GGNCC 8 cut(s) 27, 415, 433, 438, 468, 655, 678, 694
Cfr42I CCGCGG 2 cut(s) 195, 364
CpoI CGGWCCG 1 cut(s) 468
Csp6I GTAC 2 cut(s) 496, 598
CspAI ACCGGT 1 cut(s) 435
CspI CGGWCCG 1 cut(s) 468
CviAII CATG 2 cut(s) 301, 781
CviQI GTAC 2 cut(s) 496, 598
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
DraIII CACNNNGTG 1 cut(s) 94
DrdI GACNNNNNNGTC 1 cut(s) 755
DseDI GACNNNNNNGTC 1 cut(s) 755
EaeI YGGCCR 2 cut(s) 358, 624
EagI CGGCCG 2 cut(s) 358, 624
Eam1104I CTCTTC 3 cut(s) 89, 155, 242
EarI CTCTTC 3 cut(s) 89, 155, 242
EciI GGCGGA 2 cut(s) 169, 214
EclXI CGGCCG 2 cut(s) 358, 624
Eco24I GRGCYC 2 cut(s) 54, 837
Eco31I GGTCTC 1 cut(s) 763
Eco47I GGWCC 7 cut(s) 27, 433, 438, 468, 655, 678, 694
Eco52I CGGCCG 2 cut(s) 358, 624
Eco57I CTGAAG 1 cut(s) 758
EcoO109I RGGNCCY 1 cut(s) 694
EcoRII CCWGG 1 cut(s) 521
EcoT38I GRGCYC 2 cut(s) 54, 837
FaeI CATG 2 cut(s) 304, 784
FaiI YATR 4 cut(s) 71, 130, 302, 782
FaqI GGGAC 3 cut(s) 97, 554, 707
FatI CATG 2 cut(s) 300, 780
FauI CCCGC 3 cut(s) 187, 191, 759
Fnu4HI GCNGC 6 cut(s) 192, 355, 358, 361, 364, 728
FokI GGATG 2 cut(s) 353, 646
FriOI GRGCYC 2 cut(s) 54, 837
Fsp4HI GCNGC 6 cut(s) 192, 355, 358, 361, 364, 728
GlaI GCGC 1 cut(s) 299
GluI GCNGC 6 cut(s) 192, 355, 358, 361, 364, 728
GsaI CCCAGC 1 cut(s) 394
HaeIII GGCC 4 cut(s) 360, 417, 626, 776
HapII CCGG 6 cut(s) 204, 418, 436, 658, 671, 681
HhaI GCGC 1 cut(s) 300
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 2 cut(s) 304, 784
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HincII GTYRAC 2 cut(s) 316, 327
HindII GTYRAC 2 cut(s) 316, 327
HinfI GANTC 1 cut(s) 198
HpaII CCGG 6 cut(s) 204, 418, 436, 658, 671, 681
HphI GGTGA 5 cut(s) 136, 193, 205, 659, 812
Hpy166II GTNNAC 3 cut(s) 316, 327, 580
Hpy188I TCNGA 4 cut(s) 609, 763, 810, 839
Hpy188III TCNNGA 2 cut(s) 397, 710
Hpy8I GTNNAC 3 cut(s) 316, 327, 580
Hpy99I CGWCG 5 cut(s) 290, 293, 386, 428, 557
HpyAV CCTTC 5 cut(s) 259, 394, 476, 586, 787
HpyCH4III ACNGT 4 cut(s) 75, 331, 586, 613
HpyCH4IV ACGT 5 cut(s) 288, 498, 555, 705, 717
HpyCH4V TGCA 2 cut(s) 703, 730
HpyF10VI GCNNNNNNNGC 9 cut(s) 183, 188, 224, 360, 363, 414, 513, 727, 733
HpySE526I ACGT 5 cut(s) 288, 498, 555, 705, 717
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 2 cut(s) 304, 784
HspAI GCGC 1 cut(s) 298
KspI CCGCGG 2 cut(s) 195, 364
Kzo9I GATC 1 cut(s) 33
LmnI GCTCC 4 cut(s) 182, 212, 733, 763
Lsp1109I GCAGC 2 cut(s) 341, 714
LweI GCATC 3 cut(s) 375, 613, 851
MaeII ACGT 5 cut(s) 288, 498, 555, 705, 717
MaeIII GTNAC 4 cut(s) 211, 499, 769, 818
MalI GATC 1 cut(s) 35
MbiI CCGCTC 1 cut(s) 766
MboI GATC 1 cut(s) 33
MboII GAAGA 6 cut(s) 76, 142, 145, 229, 232, 751
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 2 cut(s) 54, 837
MmeI TCCRAC 1 cut(s) 411
MseI TTAA 1 cut(s) 665
MspA1I CMGCKG 3 cut(s) 194, 363, 569
MspI CCGG 6 cut(s) 204, 418, 436, 658, 671, 681
MspR9I CCNGG 5 cut(s) 419, 523, 659, 672, 682
MvaI CCWGG 1 cut(s) 523
MvnI CGCG 2 cut(s) 194, 363
MwoI GCNNNNNNNGC 9 cut(s) 183, 188, 224, 360, 363, 414, 513, 727, 733
NciI CCSGG 4 cut(s) 419, 659, 672, 682
NdeII GATC 1 cut(s) 33
NlaIII CATG 2 cut(s) 304, 784
NlaIV GGNNCC 5 cut(s) 29, 178, 208, 679, 695
NmeAIII GCCGAG 2 cut(s) 330, 652
NmuCI GTSAC 4 cut(s) 211, 499, 769, 818
NotI GCGGCCGC 1 cut(s) 358
PcsI WCGNNNNNNNCGW 2 cut(s) 495, 582
PfeI GAWTC 1 cut(s) 198
PflFI GACNNNGTC 1 cut(s) 290
PflMI CCANNNNNTGG 1 cut(s) 301
PinAI ACCGGT 1 cut(s) 435
PkrI GCNGC 6 cut(s) 193, 356, 359, 362, 365, 729
Ppu21I YACGTR 2 cut(s) 499, 718
PpuMI RGGWCCY 1 cut(s) 694
Psp5II RGGWCCY 1 cut(s) 694
Psp6I CCWGG 1 cut(s) 521
PspFI CCCAGC 1 cut(s) 390
PspGI CCWGG 1 cut(s) 521
PspN4I GGNNCC 5 cut(s) 29, 178, 208, 679, 695
PspPI GGNCC 8 cut(s) 27, 415, 433, 438, 468, 655, 678, 694
PspPPI RGGWCCY 1 cut(s) 694
PstI CTGCAG 1 cut(s) 732
PstNI CAGNNNCTG 2 cut(s) 24, 736
PsuI RGATCY 1 cut(s) 33
PsyI GACNNNGTC 1 cut(s) 290
RsaI GTAC 2 cut(s) 497, 599
RsaNI GTAC 2 cut(s) 496, 598
Rsr2I CGGWCCG 1 cut(s) 468
RsrII CGGWCCG 1 cut(s) 468
SacII CCGCGG 2 cut(s) 195, 364
SaqAI TTAA 1 cut(s) 665
SatI GCNGC 6 cut(s) 192, 355, 358, 361, 364, 728
Sau3AI GATC 1 cut(s) 33
Sau96I GGNCC 8 cut(s) 27, 415, 433, 438, 468, 655, 678, 694
ScrFI CCNGG 5 cut(s) 419, 523, 659, 672, 682
SduI GDGCHC 2 cut(s) 54, 837
SfaNI GCATC 3 cut(s) 375, 613, 851
SfcI CTRYAG 1 cut(s) 728
Sfr303I CCGCGG 2 cut(s) 195, 364
SgrBI CCGCGG 2 cut(s) 195, 364
SinI GGWCC 7 cut(s) 27, 433, 438, 468, 655, 678, 694
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
StyD4I CCNGG 5 cut(s) 417, 521, 657, 670, 680
TaaI ACNGT 4 cut(s) 75, 331, 586, 613
TaiI ACGT 5 cut(s) 291, 501, 558, 708, 720
TaqI TCGA 4 cut(s) 54, 135, 345, 552
TauI GCSGC 4 cut(s) 194, 360, 363, 366
TfiI GAWTC 1 cut(s) 198
Tru1I TTAA 1 cut(s) 665
Tru9I TTAA 1 cut(s) 665
TscAI CASTG 1 cut(s) 65
TseFI GTSAC 4 cut(s) 211, 499, 769, 818
TseI GCWGC 2 cut(s) 354, 727
Tsp45I GTSAC 4 cut(s) 211, 499, 769, 818
TspDTI ATGAA 1 cut(s) 769
TspGWI ACGGA 2 cut(s) 199, 301
TspRI CASTG 1 cut(s) 65
Tth111I GACNNNGTC 1 cut(s) 290
Van91I CCANNNNNTGG 1 cut(s) 301
VpaK11BI GGWCC 7 cut(s) 27, 433, 438, 468, 655, 678, 694
ZraI GACGTC 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.