pycom03g20450

ZINC FINGER protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009751
Physical Location & Seq
Reverse (-)
20945952 .. 20949252
3301 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1398 bp
ATGTTTCCTATCTCCATGTCCAATTCAACTTCTTTGTCTGAGGAAGGTAATAGTGTTTCTTCAGACTTTGCTGCTGCCTTAAACCCTCTTCTCCTGTCCACCAATATTTCTCCTCATCAGTATGATCAGCCACCTAACAAAATAAAAAAGAAAAGAAGCCTCCCCGGAAACCCAGACCCAGATGCTGAAGTGATTGCATTATCCCCCAAAACCCTACTTGCCACAAACAGATTTGTGTGTGAGATTTGCAACAAGGGTTTTCAGAGAGATCAGAACCTTCAGCTTCATAGGAGAGGCCACAACCTTCCATGGAAGCTGAAGCAAAGGAACAGCAAGGATCAGATCAGAAAGAGAGCCTATGTATGCCCTGAGCCCTCATGTGTTCATCACCACCCTTCAAGAGCACTTGGTGACCTCACAGGGATCAAAAAGCACTTTTGCAGGAAGCATGGGGAGAAGAAGTGGAAGTGTGACAAGTGCTCCAAGATCTATGCTGTTCAATCTGATTGGAAGGCTCACTCCAAAACCTGTGGTACAAGAGAATATAGATGTGACTGTGGAACCCTTTTCTCCAGGAAGGATAGCTTCATAACTCACAGGGCGTTCTGTGATGCTTTGGCTGAAGAAAGTGCGAGACTCTCAGCAAACCAATTTGCTACAGCCGCCATCACCACCGCCACAATTACACCTCAAATCTCCCTCTTCCCTTTTCCAACACACGGACAACAACACTTCACAAACCCAAACCCACCATTGCCACCCTTCACTACCATCTCCCTCACCCAATGGGACCCCCCACAACCTCATCAAAACCCTAACCCTAGCCCGAATCCCACCCAAAACTCCCCACTCCATCACCTCATCAAGCCCGAATCTCATCACCATTTCCCACCACCACCACAACAACCCTACAAGGGTTTGATCATCTCACCCTTCCAAAACCTCCATGTCAGCACACAACCCAACTCCAACCCCACTACGTCATCTGCCCACCTTTCAGCCACTGCACTCCTCCAGAAGGCTGCAACCGTTGGTGCAGCAGTAGCCTCTGGAGCCCAACAGGGACAGTCAGCGGGCTTGACCGTGGGAGAGTTTGGGACCGCGAGCCACATGATATCCACAGAGTATCTAGGCGGGTTCGCGTCCGGAGAGCTCGCGACATGGCGCAAGACTGACAGTCTGACAAGAGACTTTCTAGGTCTGAACGGGGATGGCACGGGGCATGATAATAATAACGTTCGCGGTGTTCGCGTCAAGGACATGCTAACGTACGCCGGTGGCGTAGGGTTCCATCAGCCGCCGTACGAGCGTCTTCGTGGCCATGATTCGCTGCTGAAGCCCCAGGGTTTCGGGTTCGCTGAGACTACTACTGCTTCGGAAACGTGGGGAGACTGTTGA

Protein Analysis

466

Amino Acids

51.09

Weight (kDa)

9.13

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2H2-2nd_BIRD-IDD PF22996 116 - 150 3.4e-21 BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD PF22995 154 - 178 2.5e-14 BIRD-IDD transcription factor, third C2HC zinc finger
C2CH-4th_BIRD-IDD PF22992 181 - 219 1.6e-23 BIRD-IDD transcription factor, fourth C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 1103, 1142, 1157, 1242, 1251
AccIII TCCGGA 1 cut(s) 1145
AciI CCGC 7 cut(s) 663, 675, 1073, 1101, 1134, 1242, 1298
AclI AACGTT 1 cut(s) 1236
AclWI GGATC 2 cut(s) 345, 431
AcoI YGGCCR 1 cut(s) 1318
AcuI CTGAAG 6 cut(s) 45, 207, 263, 338, 642, 1355
AdeI CACNNNGTG 1 cut(s) 410
AfaI GTAC 3 cut(s) 535, 1271, 1304
AfiI CCNNNNNNNGG 3 cut(s) 719, 914, 1018
AgsI TTSAA 3 cut(s) 27, 399, 500
AhdI GACNNNNNGTC 1 cut(s) 1176
AjnI CCWGG 2 cut(s) 572, 1341
AluBI AGCT 4 cut(s) 283, 316, 585, 1153
AluI AGCT 4 cut(s) 283, 316, 585, 1153
Alw21I GWGCWC 3 cut(s) 406, 482, 1155
Alw26I GTCTC 4 cut(s) 628, 1182, 1355, 1383
AlwI GGATC 2 cut(s) 345, 431
AlwNI CAGNNNCTG 2 cut(s) 185, 1004
Aor13HI TCCGGA 1 cut(s) 1145
AoxI GGCC 2 cut(s) 295, 1318
ApeKI GCWGC 5 cut(s) 71, 74, 1022, 1037, 1330
AspLEI GCGC 1 cut(s) 1167
AspS9I GGNCC 2 cut(s) 790, 1098
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 7 cut(s) 380, 422, 661, 772, 848, 872, 921
AvaII GGWCC 2 cut(s) 790, 1098
BalI TGGCCA 1 cut(s) 1320
BanII GRGCYC 3 cut(s) 375, 1057, 1155
BbsI GAAGAC 1 cut(s) 1304
Bbv12I GWGCWC 3 cut(s) 406, 482, 1155
BbvI GCAGC 5 cut(s) 58, 61, 1009, 1049, 1317
BccI CCATC 5 cut(s) 674, 779, 861, 1205, 1299
BceAI ACGGC 1 cut(s) 1285
BciT130I CCWGG 2 cut(s) 574, 1343
BclI TGATCA 2 cut(s) 124, 921
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 4 cut(s) 628, 1182, 1355, 1383
BfaI CTAG 3 cut(s) 822, 1130, 1196
BfmI CTRYAG 1 cut(s) 657
BglII AGATCT 1 cut(s) 486
BisI GCNGC 7 cut(s) 72, 75, 663, 1023, 1038, 1298, 1331
BlsI GCNGC 7 cut(s) 73, 76, 664, 1024, 1039, 1299, 1332
Bme1390I CCNGG 3 cut(s) 165, 574, 1343
Bme18I GGWCC 2 cut(s) 790, 1098
BmeRI GACNNNNNGTC 1 cut(s) 1176
BmgT120I GGNCC 2 cut(s) 790, 1098
BmiI GGNNCC 6 cut(s) 562, 791, 792, 1054, 1099, 1289
BmrFI CCNGG 3 cut(s) 165, 574, 1343
BmsI GCATC 2 cut(s) 172, 601
BpiI GAAGAC 1 cut(s) 1304
BpmI CTGGAG 3 cut(s) 556, 998, 1071
Bpu10I CCTNAGC 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 165
BsaJI CCNNGG 5 cut(s) 163, 308, 1083, 1341, 1342
BsaWI WCCGGW 1 cut(s) 1145
BsaXI ACNNNNNCTCC 4 cut(s) 446, 464, 476, 494
Bsc4I CCNNNNNNNGG 3 cut(s) 719, 914, 1018
Bse118I RCCGGY 1 cut(s) 1274
Bse3DI GCAATG 1 cut(s) 752
BseAI TCCGGA 1 cut(s) 1145
BseBI CCWGG 2 cut(s) 574, 1343
BseDI CCNNGG 5 cut(s) 163, 308, 1083, 1341, 1342
BseGI GGATG 1 cut(s) 1216
BseLI CCNNNNNNNGG 3 cut(s) 719, 914, 1018
BseMI GCAATG 1 cut(s) 752
BseMII CTCAG 4 cut(s) 30, 360, 654, 1350
BseRI GAGGAG 2 cut(s) 102, 1001
BseXI GCAGC 5 cut(s) 58, 61, 1009, 1049, 1317
BsgI GTGCAG 2 cut(s) 990, 1056
Bsh1236I CGCG 5 cut(s) 1103, 1142, 1157, 1242, 1251
BshFI GGCC 2 cut(s) 297, 1320
BsiHKAI GWGCWC 3 cut(s) 406, 482, 1155
BsiSI CCGG 3 cut(s) 165, 1146, 1275
BsiWI CGTACG 2 cut(s) 1269, 1302
BslFI GGGAC 3 cut(s) 803, 1077, 1111
BslI CCNNNNNNNGG 3 cut(s) 719, 914, 1018
BsmAI GTCTC 4 cut(s) 628, 1182, 1355, 1383
BsmFI GGGAC 3 cut(s) 803, 1077, 1111
BsnI GGCC 2 cut(s) 297, 1320
Bsp1286I GDGCHC 5 cut(s) 375, 406, 482, 1057, 1155
Bsp13I TCCGGA 1 cut(s) 1145
Bsp143I GATC 7 cut(s) 124, 268, 337, 342, 423, 486, 921
Bsp19I CCATGG 1 cut(s) 308
Bsp68I TCGCGA 1 cut(s) 1157
BspACI CCGC 7 cut(s) 663, 675, 1073, 1101, 1134, 1242, 1298
BspANI GGCC 2 cut(s) 297, 1320
BspCNI CTCAG 4 cut(s) 31, 361, 653, 1351
BspEI TCCGGA 1 cut(s) 1145
BspFNI CGCG 5 cut(s) 1103, 1142, 1157, 1242, 1251
BspLI GGNNCC 6 cut(s) 562, 791, 792, 1054, 1099, 1289
BspPI GGATC 2 cut(s) 345, 431
BsrDI GCAATG 1 cut(s) 752
BsrFI RCCGGY 1 cut(s) 1274
BssAI RCCGGY 1 cut(s) 1274
BssECI CCNNGG 5 cut(s) 163, 308, 1083, 1341, 1342
BssMI GATC 7 cut(s) 124, 268, 337, 342, 423, 486, 921
BssT1I CCWWGG 1 cut(s) 308
Bst2UI CCWGG 2 cut(s) 574, 1343
Bst4CI ACNGT 6 cut(s) 557, 1030, 1068, 1084, 1178, 1394
Bst6I CTCTTC 2 cut(s) 93, 707
BstC8I GCNNGC 3 cut(s) 1075, 1105, 1155
BstDEI CTNAG 4 cut(s) 39, 369, 640, 1359
BstDSI CCRYGG 2 cut(s) 308, 1083
BstEII GGTNACC 1 cut(s) 410
BstENI CCTNNNNNAGG 1 cut(s) 1016
BstF5I GGATG 1 cut(s) 1216
BstFNI CGCG 5 cut(s) 1103, 1142, 1157, 1242, 1251
BstHHI GCGC 1 cut(s) 1167
BstKTI GATC 7 cut(s) 127, 271, 340, 345, 426, 489, 924
BstMAI GTCTC 4 cut(s) 628, 1182, 1355, 1383
BstMBI GATC 7 cut(s) 124, 268, 337, 342, 423, 486, 921
BstMWI GCNNNNNNNGC 6 cut(s) 662, 1043, 1052, 1248, 1306, 1336
BstNI CCWGG 2 cut(s) 574, 1343
BstNSI RCATGY 1 cut(s) 1264
BstPI GGTNACC 1 cut(s) 410
BstSCI CCNGG 3 cut(s) 163, 572, 1341
BstSFI CTRYAG 1 cut(s) 657
BstUI CGCG 5 cut(s) 1103, 1142, 1157, 1242, 1251
BstV1I GCAGC 5 cut(s) 58, 61, 1009, 1049, 1317
BstV2I GAAGAC 1 cut(s) 1304
BstX2I RGATCY 1 cut(s) 486
BstYI RGATCY 1 cut(s) 486
BsuRI GGCC 2 cut(s) 297, 1320
BtgI CCRYGG 2 cut(s) 308, 1083
BtsCI GGATG 1 cut(s) 1216
BtsI GCAGTG 1 cut(s) 1002
BtsIMutI CAGTG 1 cut(s) 1002
BtuMI TCGCGA 1 cut(s) 1157
Cac8I GCNNGC 3 cut(s) 1075, 1105, 1155
CaiI CAGNNNCTG 2 cut(s) 185, 1004
CfoI GCGC 1 cut(s) 1167
Cfr10I RCCGGY 1 cut(s) 1274
Cfr13I GGNCC 2 cut(s) 790, 1098
CseI GACGC 3 cut(s) 1131, 1240, 1298
Csp6I GTAC 3 cut(s) 534, 1270, 1303
CspCI CAANNNNNGTGG 2 cut(s) 511, 546
CviQI GTAC 3 cut(s) 534, 1270, 1303
DdeI CTNAG 4 cut(s) 39, 369, 640, 1359
DpnI GATC 7 cut(s) 126, 270, 339, 344, 425, 488, 923
DpnII GATC 7 cut(s) 124, 268, 337, 342, 423, 486, 921
DraIII CACNNNGTG 1 cut(s) 410
DriI GACNNNNNGTC 1 cut(s) 1176
EaeI YGGCCR 1 cut(s) 1318
Eam1104I CTCTTC 2 cut(s) 93, 707
Eam1105I GACNNNNNGTC 1 cut(s) 1176
EarI CTCTTC 2 cut(s) 93, 707
Ecl136II GAGCTC 1 cut(s) 1153
Eco130I CCWWGG 1 cut(s) 308
Eco24I GRGCYC 3 cut(s) 375, 1057, 1155
Eco32I GATATC 1 cut(s) 1116
Eco47I GGWCC 2 cut(s) 790, 1098
Eco53kI GAGCTC 1 cut(s) 1153
Eco57I CTGAAG 6 cut(s) 45, 207, 263, 338, 642, 1355
Eco91I GGTNACC 1 cut(s) 410
EcoICRI GAGCTC 1 cut(s) 1153
EcoNI CCTNNNNNAGG 1 cut(s) 1016
EcoO109I RGGNCCY 1 cut(s) 790
EcoO65I GGTNACC 1 cut(s) 410
EcoRII CCWGG 2 cut(s) 572, 1341
EcoRV GATATC 1 cut(s) 1116
EcoT14I CCWWGG 1 cut(s) 308
EcoT38I GRGCYC 3 cut(s) 375, 1057, 1155
ErhI CCWWGG 1 cut(s) 308
FalI AAGNNNNNCTT 2 cut(s) 569, 601
FaqI GGGAC 3 cut(s) 803, 1077, 1111
FauI CCCGC 2 cut(s) 1066, 1127
FbaI TGATCA 2 cut(s) 124, 921
Fnu4HI GCNGC 7 cut(s) 72, 75, 663, 1023, 1038, 1298, 1331
FokI GGATG 1 cut(s) 1223
FriOI GRGCYC 3 cut(s) 375, 1057, 1155
Fsp4HI GCNGC 7 cut(s) 72, 75, 663, 1023, 1038, 1298, 1331
FspBI CTAG 3 cut(s) 822, 1130, 1196
GlaI GCGC 1 cut(s) 1166
GluI GCNGC 7 cut(s) 72, 75, 663, 1023, 1038, 1298, 1331
GsuI CTGGAG 3 cut(s) 556, 998, 1071
HaeIII GGCC 2 cut(s) 297, 1320
HapII CCGG 3 cut(s) 165, 1146, 1275
HgaI GACGC 3 cut(s) 1131, 1240, 1298
HhaI GCGC 1 cut(s) 1167
Hin6I GCGC 1 cut(s) 1165
HinP1I GCGC 1 cut(s) 1165
HinfI GANTC 4 cut(s) 636, 829, 872, 1325
HpaII CCGG 3 cut(s) 165, 1146, 1275
HphI GGTGA 7 cut(s) 380, 422, 661, 772, 848, 872, 921
Hpy166II GTNNAC 1 cut(s) 99
Hpy188III TCNNGA 5 cut(s) 399, 1015, 1050, 1146, 1156
Hpy8I GTNNAC 1 cut(s) 99
HpyAV CCTTC 9 cut(s) 38, 287, 314, 405, 505, 571, 772, 943, 1012
HpyCH4III ACNGT 6 cut(s) 557, 1030, 1068, 1084, 1178, 1394
HpyCH4IV ACGT 4 cut(s) 980, 1236, 1268, 1382
HpyCH4V TGCA 6 cut(s) 197, 249, 441, 1007, 1025, 1037
HpyF10VI GCNNNNNNNGC 6 cut(s) 662, 1043, 1052, 1248, 1306, 1336
HpyF3I CTNAG 4 cut(s) 39, 369, 640, 1359
HpySE526I ACGT 4 cut(s) 980, 1236, 1268, 1382
HspAI GCGC 1 cut(s) 1165
KflI GGGWCCC 1 cut(s) 790
Kpn2I TCCGGA 1 cut(s) 1145
Ksp22I TGATCA 2 cut(s) 124, 921
Kzo9I GATC 7 cut(s) 124, 268, 337, 342, 423, 486, 921
LmnI GCTCC 2 cut(s) 485, 1052
Lsp1109I GCAGC 5 cut(s) 58, 61, 1009, 1049, 1317
LweI GCATC 2 cut(s) 172, 601
MaeI CTAG 3 cut(s) 822, 1130, 1196
MaeII ACGT 4 cut(s) 980, 1236, 1268, 1382
MaeIII GTNAC 3 cut(s) 410, 470, 551
MalI GATC 7 cut(s) 126, 270, 339, 344, 425, 488, 923
MboI GATC 7 cut(s) 124, 268, 337, 342, 423, 486, 921
MboII GAAGA 6 cut(s) 51, 80, 469, 635, 694, 1304
MflI RGATCY 1 cut(s) 486
MhlI GDGCHC 5 cut(s) 375, 406, 482, 1057, 1155
MlsI TGGCCA 1 cut(s) 1320
MluCI AATT 3 cut(s) 22, 650, 681
MluNI TGGCCA 1 cut(s) 1320
MlyI GAGTC 1 cut(s) 630
MmeI TCCRAC 2 cut(s) 737, 993
Mox20I TGGCCA 1 cut(s) 1320
MroI TCCGGA 1 cut(s) 1145
MscI TGGCCA 1 cut(s) 1320
MseI TTAA 1 cut(s) 80
MslI CAYNNNNRTG 1 cut(s) 120
Msp20I TGGCCA 1 cut(s) 1320
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 3 cut(s) 165, 1146, 1275
MspR9I CCNGG 3 cut(s) 165, 574, 1343
MvaI CCWGG 2 cut(s) 574, 1343
MvnI CGCG 5 cut(s) 1103, 1142, 1157, 1242, 1251
MwoI GCNNNNNNNGC 6 cut(s) 662, 1043, 1052, 1248, 1306, 1336
NciI CCSGG 1 cut(s) 165
NcoI CCATGG 1 cut(s) 308
NdeII GATC 7 cut(s) 124, 268, 337, 342, 423, 486, 921
NlaIV GGNNCC 6 cut(s) 562, 791, 792, 1054, 1099, 1289
NmuCI GTSAC 3 cut(s) 410, 470, 551
NruI TCGCGA 1 cut(s) 1157
NspI RCATGY 1 cut(s) 1264
PasI CCCWGGG 1 cut(s) 1342
PcsI WCGNNNNNNNCGW 1 cut(s) 1278
PfeI GAWTC 3 cut(s) 829, 872, 1325
Pfl23II CGTACG 2 cut(s) 1269, 1302
PfoI TCCNGGA 1 cut(s) 572
PkrI GCNGC 7 cut(s) 73, 76, 664, 1024, 1039, 1299, 1332
PleI GAGTC 1 cut(s) 630
PpsI GAGTC 1 cut(s) 630
PpuMI RGGWCCY 1 cut(s) 790
Psp124BI GAGCTC 1 cut(s) 1155
Psp1406I AACGTT 1 cut(s) 1236
Psp5II RGGWCCY 1 cut(s) 790
Psp6I CCWGG 2 cut(s) 572, 1341
PspEI GGTNACC 1 cut(s) 410
PspGI CCWGG 2 cut(s) 572, 1341
PspLI CGTACG 2 cut(s) 1269, 1302
PspN4I GGNNCC 6 cut(s) 562, 791, 792, 1054, 1099, 1289
PspPI GGNCC 2 cut(s) 790, 1098
PspPPI RGGWCCY 1 cut(s) 790
PstNI CAGNNNCTG 2 cut(s) 185, 1004
PsuI RGATCY 1 cut(s) 486
RruI TCGCGA 1 cut(s) 1157
RsaI GTAC 3 cut(s) 535, 1271, 1304
RsaNI GTAC 3 cut(s) 534, 1270, 1303
RseI CAYNNNNRTG 1 cut(s) 120
SacI GAGCTC 1 cut(s) 1155
SaqAI TTAA 1 cut(s) 80
SatI GCNGC 7 cut(s) 72, 75, 663, 1023, 1038, 1298, 1331
Sau3AI GATC 7 cut(s) 124, 268, 337, 342, 423, 486, 921
Sau96I GGNCC 2 cut(s) 790, 1098
SchI GAGTC 1 cut(s) 630
ScrFI CCNGG 3 cut(s) 165, 574, 1343
SduI GDGCHC 5 cut(s) 375, 406, 482, 1057, 1155
SfaNI GCATC 2 cut(s) 172, 601
SfcI CTRYAG 1 cut(s) 657
SgrAI CRCCGGYG 1 cut(s) 1274
SinI GGWCC 2 cut(s) 790, 1098
SmiMI CAYNNNNRTG 1 cut(s) 120
Sse9I AATT 3 cut(s) 22, 650, 681
SsiI CCGC 7 cut(s) 663, 675, 1073, 1101, 1134, 1242, 1298
SspI AATATT 1 cut(s) 106
SspMI CTAG 3 cut(s) 822, 1130, 1196
SstI GAGCTC 1 cut(s) 1155
StyD4I CCNGG 3 cut(s) 163, 572, 1341
StyI CCWWGG 1 cut(s) 308
TaaI ACNGT 6 cut(s) 557, 1030, 1068, 1084, 1178, 1394
TaiI ACGT 4 cut(s) 983, 1239, 1271, 1385
TasI AATT 3 cut(s) 22, 650, 681
TauI GCSGC 2 cut(s) 665, 1300
TfiI GAWTC 3 cut(s) 829, 872, 1325
Tru1I TTAA 1 cut(s) 80
Tru9I TTAA 1 cut(s) 80
TscAI CASTG 1 cut(s) 1009
TseFI GTSAC 3 cut(s) 410, 470, 551
TseI GCWGC 5 cut(s) 71, 74, 1022, 1037, 1330
Tsp45I GTSAC 3 cut(s) 410, 470, 551
TspDTI ATGAA 3 cut(s) 275, 374, 577
TspGWI ACGGA 1 cut(s) 735
TspRI CASTG 1 cut(s) 1009
VpaK11BI GGWCC 2 cut(s) 790, 1098
XagI CCTNNNNNAGG 1 cut(s) 1016
XceI RCATGY 1 cut(s) 1264
XspI CTAG 3 cut(s) 822, 1130, 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.