pycom03g22970

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0015829
Physical Location & Seq
Reverse (-)
22966954 .. 22967436
483 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22970.1

Sequence Viewer

Length: 483 bp
ATGGCTCCATCAATGGTGTTTCTCGTTGCCGTTCTCGTGGCCATTCTCTCATCCTCGTTCGCCTATGGCGACAACAACCCATCGGCATACGTGGTGCTTCAAGAATACGACTTCCCGATCGGCCTTCTTCCCAAAGGAGTAACAGGGTACGAATTAGACAGAGAATCTGGAAATTTCAAGGCTTATTTTGACGGAACTTGCCGCTTCTCCATTGAAAATTCATACAACCTCAGGTACAAATCCACCATAACTGGTGTTTTGTCCAAGGGCAGGCTCAAAAATTTGGATGGCGTCAGTGTTAAGATACTATTATTTTGGCTCAACATTGTCGAAATTATTCGCGATGGCGACGACCTACAATTCTCTGTGGGGATTGCTTCGGCAGACTTTTCCATTGACAACTTTGAGGAGAGTCCGCAGTGTGGGTGTGGATTTGCTTGTAAGAGTAGAGCTAGCTCTACAAGTAGAACTGCGCTGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

161

Amino Acids

17.49

Weight (kDa)

4.92

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 30 - 136 1.3e-35 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015829)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02360
fragaria_vesca FvH4_2g25890
malus_domestica MD03G1290400.v1.1 MD11G1308100.v1.1
prunus_persica Prupe.8G263400_v2.0.a1
pyrus_communis pycom03g22970
rosa_chinensis RchiOBHm_Chr6g0294221
rosa_laevigata RLG00000011889
rosa_roxburghii Rroxscaffold_7G00173540
rosa_rugosa Rorug06G0242600
rosa_samantha Rh6AG354800 Rh6BG361700 Rh6CG369000 Rh6DG355800
rosa_wichuraiana Rw6G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 342
AciI CCGC 2 cut(s) 202, 416
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 3 cut(s) 172, 217, 280
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 2 cut(s) 149, 236
AfiI CCNNNNNNNGG 2 cut(s) 270, 422
AgsI TTSAA 3 cut(s) 101, 178, 215
AluBI AGCT 2 cut(s) 452, 456
AluI AGCT 2 cut(s) 452, 456
AoxI GGCC 2 cut(s) 39, 121
ApoI RAATTY 3 cut(s) 172, 217, 280
Asp700I GAANNNNTTC 1 cut(s) 336
AspLEI GCGC 1 cut(s) 475
AsuNHI GCTAGC 1 cut(s) 452
AxyI CCTNAGG 1 cut(s) 230
BalI TGGCCA 1 cut(s) 41
BauI CACGAG 1 cut(s) 35
BccI CCATC 4 cut(s) 16, 88, 281, 338
BceAI ACGGC 1 cut(s) 14
BfaI CTAG 2 cut(s) 453, 481
BisI GCNGC 1 cut(s) 202
BlsI GCNGC 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 6
BmtI GCTAGC 1 cut(s) 456
BsaAI YACGTR 1 cut(s) 91
BsaHI GRCGYC 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 264
Bsc4I CCNNNNNNNGG 2 cut(s) 270, 422
Bse1I ACTGG 1 cut(s) 256
Bse21I CCTNAGG 1 cut(s) 230
BseDI CCNNGG 1 cut(s) 264
BseGI GGATG 2 cut(s) 50, 292
BseLI CCNNNNNNNGG 2 cut(s) 270, 422
BseMII CTCAG 1 cut(s) 244
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 1 cut(s) 422
Bsh1236I CGCG 1 cut(s) 342
Bsh1285I CGRYCG 1 cut(s) 120
BshFI GGCC 2 cut(s) 41, 123
BsiEI CGRYCG 1 cut(s) 120
BslI CCNNNNNNNGG 2 cut(s) 270, 422
BsnI GGCC 2 cut(s) 41, 123
Bsp143I GATC 1 cut(s) 117
Bsp68I TCGCGA 1 cut(s) 342
BspACI CCGC 2 cut(s) 202, 416
BspANI GGCC 2 cut(s) 41, 123
BspCNI CTCAG 1 cut(s) 243
BspFNI CGCG 1 cut(s) 342
BspLI GGNNCC 1 cut(s) 6
BspOI GCTAGC 1 cut(s) 456
BsrI ACTGG 1 cut(s) 256
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 1 cut(s) 117
BssNI GRCGYC 1 cut(s) 291
BssSI CACGAG 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 264
Bst2BI CACGAG 1 cut(s) 35
BstACI GRCGYC 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 91
BstC8I GCNNGC 2 cut(s) 272, 454
BstDEI CTNAG 1 cut(s) 230
BstF5I GGATG 2 cut(s) 50, 292
BstFNI CGCG 1 cut(s) 342
BstHHI GCGC 1 cut(s) 475
BstKTI GATC 1 cut(s) 120
BstMBI GATC 1 cut(s) 117
BstMCI CGRYCG 1 cut(s) 120
BstUI CGCG 1 cut(s) 342
Bsu36I CCTNAGG 1 cut(s) 230
BsuRI GGCC 2 cut(s) 41, 123
BtgZI GCGATG 1 cut(s) 357
BtsCI GGATG 2 cut(s) 50, 292
BtsI GCAGTG 1 cut(s) 425
BtsIMutI CAGTG 2 cut(s) 301, 425
BtuMI TCGCGA 1 cut(s) 342
Cac8I GCNNGC 2 cut(s) 272, 454
CfoI GCGC 1 cut(s) 475
CseI GACGC 1 cut(s) 280
Csp6I GTAC 2 cut(s) 148, 235
CviJI RGCY 8 cut(s) 5, 41, 123, 182, 274, 319, 452, 456
CviKI_1 RGCY 8 cut(s) 5, 41, 123, 182, 274, 319, 452, 456
CviQI GTAC 2 cut(s) 148, 235
DdeI CTNAG 1 cut(s) 230
DpnI GATC 1 cut(s) 119
DpnII GATC 1 cut(s) 117
EaeI YGGCCR 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 264
Eco81I CCTNAGG 1 cut(s) 230
EcoT14I CCWWGG 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 264
FaiI YATR 4 cut(s) 66, 88, 223, 248
Fnu4HI GCNGC 1 cut(s) 202
FokI GGATG 2 cut(s) 37, 299
Fsp4HI GCNGC 1 cut(s) 202
FspBI CTAG 2 cut(s) 453, 481
GlaI GCGC 1 cut(s) 474
GluI GCNGC 1 cut(s) 202
HaeIII GGCC 2 cut(s) 41, 123
HgaI GACGC 1 cut(s) 280
HhaI GCGC 1 cut(s) 475
Hin1I GRCGYC 1 cut(s) 291
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HinfI GANTC 2 cut(s) 164, 412
Hpy188III TCNNGA 4 cut(s) 101, 115, 168, 341
Hpy99I CGWCG 1 cut(s) 353
HpyAV CCTTC 1 cut(s) 134
HpyCH4IV ACGT 1 cut(s) 90
HpyF3I CTNAG 1 cut(s) 230
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 291
HspAI GCGC 1 cut(s) 473
Kzo9I GATC 1 cut(s) 117
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 5 cut(s) 129, 153, 217, 237, 256
MaeI CTAG 2 cut(s) 453, 481
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 139
MalI GATC 1 cut(s) 119
MboI GATC 1 cut(s) 117
MboII GAAGA 1 cut(s) 119
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 6 cut(s) 152, 172, 217, 280, 333, 359
MluNI TGGCCA 1 cut(s) 41
MlyI GAGTC 1 cut(s) 421
MnlI CCTC 3 cut(s) 64, 239, 400
Mox20I TGGCCA 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 336
MscI TGGCCA 1 cut(s) 41
MseI TTAA 1 cut(s) 300
Msp20I TGGCCA 1 cut(s) 41
MvnI CGCG 1 cut(s) 342
NdeII GATC 1 cut(s) 117
NheI GCTAGC 1 cut(s) 452
NlaIV GGNNCC 1 cut(s) 6
NruI TCGCGA 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PdmI GAANNNNTTC 1 cut(s) 336
PfeI GAWTC 1 cut(s) 164
PkrI GCNGC 1 cut(s) 203
Ple19I CGATCG 1 cut(s) 120
PleI GAGTC 1 cut(s) 420
PpsI GAGTC 1 cut(s) 420
Ppu21I YACGTR 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 6
PvuI CGATCG 1 cut(s) 120
RruI TCGCGA 1 cut(s) 342
RsaI GTAC 2 cut(s) 149, 236
RsaNI GTAC 2 cut(s) 148, 235
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 1 cut(s) 202
Sau3AI GATC 1 cut(s) 117
SchI GAGTC 1 cut(s) 421
SetI ASST 7 cut(s) 93, 231, 236, 357, 454, 458, 482
Sse9I AATT 6 cut(s) 152, 172, 217, 280, 333, 359
SsiI CCGC 2 cut(s) 202, 416
SspMI CTAG 2 cut(s) 453, 481
StyI CCWWGG 1 cut(s) 264
TaiI ACGT 1 cut(s) 93
TaqI TCGA 1 cut(s) 330
TasI AATT 6 cut(s) 152, 172, 217, 280, 333, 359
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 2 cut(s) 301, 425
TspDTI ATGAA 1 cut(s) 210
TspGWI ACGGA 1 cut(s) 207
TspRI CASTG 2 cut(s) 301, 425
XapI RAATTY 3 cut(s) 172, 217, 280
XmnI GAANNNNTTC 1 cut(s) 336
XspI CTAG 2 cut(s) 453, 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.