pycom04g08660

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0010678
Physical Location & Seq
Reverse (-)
10529463 .. 10530488
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g08660.1

Sequence Viewer

Length: 1026 bp
ATGGTCATGGTCTTCGGTGAGATCACAACAAAAGCGAAAGTAGATTACGAGAAAATAGTCCGAGACACTTGCAGAGGAATTGGGTTTACATCAGCTGATGTAGGCCTTGATGCTGACAACTGCAAAGTCCTCGTCAACATTGAGAAACAAAGCCCAGAAATTGCAGAGGGAGTTCATGGTCACCTCACCAAGAAGCCCGAGGAAATTGGAGCTGGCGACCAAGGCCACATGTTTGGTTATGCCACTGATGAGACACCTGAGTTCATGCCACTCACCCATGTCCTTGCTACCAAGATTGGTGCCAAGCTTACGGAGGTCAGAAAGAATAAGACGGTCCCATGGCTGAGGCCCGATGGTAAGACCCAAGTGACTGTTGAGTACCAGAACGAGAACGGGGCCATGGTCCCTATTCAGGTGCACACCATCCTCATCTCAACCCAACATGATGAGACTGTCACAAATGAGCAGATTGCTGCTGATTTGAAGGAACATGTGATCGATCCTGTCGTCCCAGCTCAATTCATTGATGACAAAACTATCTACCACTTGAACCCTTCAGGTCGATTCGTCATTGGAGGACCCCATGGAGATGCTGGGCTCACTGGCCGGAAGATAATTATAGACACCTATGGTGGTTGGGGTGCTCATGGTGGTGGTGCTTTCTCTGGAAAAGATCCAACCAAGGTTGACCGTAGTGGTGCATACATTGTAAGGCAGGCAGCAAAAAGTGTTGTTGCATCCGGGCTTGCTCGCCGCTGTATTGTTCAGGTTTCTTATGCAATTGGCGTCCCAGATCCCCTGTCGGTTTTCGTGGATACCTACAAAACTGGAAAAATCCCGGACAAGGACATATTGGTTCTCATCAAGGAGAACTTTGACTTTAGGCCGGGAATGATTGCCCTCAACCTTGATTTGAAAAGAGGAGGCAACTTCAGGTACCAAAAGACTGCTGCTTATGGTCATTTTGGCCGCGATGATCCAGATTTCACATGGGAGACCATCAAGATCCTCAAGCCAAAGGCTTAA

Protein Analysis

342

Amino Acids

37.31

Weight (kDa)

6.6

Isoelectric Point (pI)

17.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 1 - 52 1.8e-12 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 68 - 189 1.6e-47 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 191 - 332 6.7e-61 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36880 AT2G36880
fragaria_vesca FvH4_6g16510
malus_domestica MD04G1091900.v1.1 MD12G1109900.v1.1
prunus_persica Prupe.7G128500_v2.0.a1
pyrus_communis pycom04g08660 pycom12g11080
rosa_chinensis RchiOBHm_Chr3g0470421
rosa_laevigata RLG00000024269
rosa_multiflora Rmu_sc0002383.1_g000009 Rmu_sc0020134.1_g000003
rosa_roxburghii Rroxscaffold_6G00410830
rosa_rugosa Rorug03G0108600 Rorug03G0108700
rosa_samantha Rh3AG159100 Rh3BG183600 Rh3CG174100 Rh3DG191200
rosa_wichuraiana Rw3G015070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 936
AccB1I GGYRCC 2 cut(s) 299, 936
AccII CGCG 1 cut(s) 972
AciI CCGC 2 cut(s) 754, 970
AclWI GGATC 5 cut(s) 494, 668, 788, 971, 1000
AcoI YGGCCR 2 cut(s) 604, 967
AcuI CTGAAG 2 cut(s) 540, 916
AcyI GRCGYC 1 cut(s) 786
AfaI GTAC 2 cut(s) 380, 938
AfiI CCNNNNNNNGG 2 cut(s) 412, 844
AflIII ACRYGT 2 cut(s) 228, 490
AgsI TTSAA 3 cut(s) 484, 550, 916
AluBI AGCT 4 cut(s) 95, 212, 307, 515
AluI AGCT 4 cut(s) 95, 212, 307, 515
Alw21I GWGCWC 2 cut(s) 420, 646
Alw26I GTCTC 4 cut(s) 57, 245, 443, 989
Alw44I GTGCAC 1 cut(s) 416
AlwI GGATC 5 cut(s) 494, 668, 788, 971, 1000
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 7 cut(s) 103, 223, 347, 396, 604, 884, 967
ApaLI GTGCAC 1 cut(s) 416
ApeKI GCWGC 3 cut(s) 473, 719, 950
ArsI GACNNNNNNTTYG 2 cut(s) 117, 149
Asp718I GGTACC 1 cut(s) 936
AspS9I GGNCC 5 cut(s) 334, 348, 396, 403, 578
AsuC2I CCSGG 3 cut(s) 742, 839, 888
AsuHPI GGTGA 4 cut(s) 29, 173, 178, 265
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 3 cut(s) 334, 403, 578
BaeGI GKGCMC 1 cut(s) 420
BanI GGYRCC 2 cut(s) 299, 936
BanII GRGCYC 1 cut(s) 600
BbsI GAAGAC 1 cut(s) 4
Bbv12I GWGCWC 2 cut(s) 420, 646
BbvCI CCTCAGC 1 cut(s) 344
BbvI GCAGC 3 cut(s) 460, 731, 937
BccI CCATC 3 cut(s) 347, 431, 1007
BcgI CGANNNNNNTGC 4 cut(s) 51, 85, 112, 146
BciVI GTATCC 1 cut(s) 808
BcnI CCSGG 3 cut(s) 742, 839, 888
BcoDI GTCTC 4 cut(s) 57, 245, 443, 989
BfuI GTATCC 1 cut(s) 808
BisI GCNGC 5 cut(s) 474, 720, 754, 951, 970
BlsI GCNGC 5 cut(s) 475, 721, 755, 952, 971
Bme1390I CCNGG 3 cut(s) 742, 839, 888
Bme18I GGWCC 3 cut(s) 334, 403, 578
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 5 cut(s) 334, 348, 396, 403, 578
BmiI GGNNCC 6 cut(s) 301, 336, 397, 405, 580, 938
BmrFI CCNGG 3 cut(s) 742, 839, 888
BmsI GCATC 3 cut(s) 100, 580, 746
BpiI GAAGAC 1 cut(s) 4
Bpu10I CCTNAGC 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 995
BpuMI CCSGG 3 cut(s) 742, 839, 888
Bsa29I ATCGAT 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 786
BsaI GGTCTC 1 cut(s) 989
BsaJI CCNNGG 6 cut(s) 198, 220, 338, 399, 583, 681
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 844
Bse1I ACTGG 2 cut(s) 607, 832
BseCI ATCGAT 1 cut(s) 498
BseDI CCNNGG 6 cut(s) 198, 220, 338, 399, 583, 681
BseGI GGATG 2 cut(s) 423, 737
BseLI CCNNNNNNNGG 2 cut(s) 412, 844
BseMII CTCAG 2 cut(s) 249, 335
BseNI ACTGG 2 cut(s) 607, 832
BseRI GAGGAG 1 cut(s) 936
BseSI GKGCMC 1 cut(s) 420
BseXI GCAGC 3 cut(s) 460, 731, 937
BseYI CCCAGC 2 cut(s) 511, 593
Bsh1236I CGCG 1 cut(s) 972
BshFI GGCC 7 cut(s) 105, 225, 349, 398, 606, 886, 969
BshNI GGYRCC 2 cut(s) 299, 936
BshVI ATCGAT 1 cut(s) 498
BsiHKAI GWGCWC 2 cut(s) 420, 646
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 4 cut(s) 607, 741, 839, 887
BslFI GGGAC 4 cut(s) 320, 389, 494, 773
BslI CCNNNNNNNGG 2 cut(s) 412, 844
BsmAI GTCTC 4 cut(s) 57, 245, 443, 989
BsmFI GGGAC 4 cut(s) 320, 389, 494, 773
BsnI GGCC 7 cut(s) 105, 225, 349, 398, 606, 886, 969
Bso31I GGTCTC 1 cut(s) 989
BsoBI CYCGRG 1 cut(s) 197
Bsp1286I GDGCHC 3 cut(s) 420, 600, 646
Bsp143I GATC 7 cut(s) 21, 495, 499, 673, 793, 976, 1005
Bsp19I CCATGG 3 cut(s) 338, 399, 583
BspACI CCGC 2 cut(s) 754, 970
BspANI GGCC 7 cut(s) 105, 225, 349, 398, 606, 886, 969
BspCNI CTCAG 2 cut(s) 250, 336
BspDI ATCGAT 1 cut(s) 498
BspFNI CGCG 1 cut(s) 972
BspLI GGNNCC 6 cut(s) 301, 336, 397, 405, 580, 938
BspPI GGATC 5 cut(s) 494, 668, 788, 971, 1000
BspT107I GGYRCC 2 cut(s) 299, 936
BspTNI GGTCTC 1 cut(s) 989
BsrI ACTGG 2 cut(s) 607, 832
BssECI CCNNGG 6 cut(s) 198, 220, 338, 399, 583, 681
BssMI GATC 7 cut(s) 21, 495, 499, 673, 793, 976, 1005
BssNI GRCGYC 1 cut(s) 786
BssT1I CCWWGG 5 cut(s) 220, 338, 399, 583, 681
Bst4CI ACNGT 4 cut(s) 334, 373, 454, 692
BstACI GRCGYC 1 cut(s) 786
BstC8I GCNNGC 4 cut(s) 214, 717, 747, 751
BstDEI CTNAG 2 cut(s) 258, 344
BstDSI CCRYGG 3 cut(s) 338, 399, 583
BstEII GGTNACC 1 cut(s) 179
BstF5I GGATG 2 cut(s) 423, 737
BstFNI CGCG 1 cut(s) 972
BstKTI GATC 7 cut(s) 24, 498, 502, 676, 796, 979, 1008
BstMAI GTCTC 4 cut(s) 57, 245, 443, 989
BstMBI GATC 7 cut(s) 21, 495, 499, 673, 793, 976, 1005
BstMWI GCNNNNNNNGC 1 cut(s) 222
BstNSI RCATGY 2 cut(s) 232, 494
BstPI GGTNACC 1 cut(s) 179
BstSCI CCNGG 3 cut(s) 740, 837, 886
BstSLI GKGCMC 1 cut(s) 420
BstUI CGCG 1 cut(s) 972
BstV1I GCAGC 3 cut(s) 460, 731, 937
BstV2I GAAGAC 1 cut(s) 4
BstX2I RGATCY 3 cut(s) 673, 793, 1005
BstXI CCANNNNNNTGG 1 cut(s) 233
BstYI RGATCY 3 cut(s) 673, 793, 1005
Bsu15I ATCGAT 1 cut(s) 498
BsuI GTATCC 1 cut(s) 808
BsuRI GGCC 7 cut(s) 105, 225, 349, 398, 606, 886, 969
BsuTUI ATCGAT 1 cut(s) 498
BtgI CCRYGG 3 cut(s) 338, 399, 583
BtgZI GCGATG 1 cut(s) 987
BtsCI GGATG 2 cut(s) 423, 737
BtsIMutI CAGTG 2 cut(s) 243, 600
Cac8I GCNNGC 4 cut(s) 214, 717, 747, 751
Cfr13I GGNCC 5 cut(s) 334, 348, 396, 403, 578
ClaI ATCGAT 1 cut(s) 498
CseI GACGC 1 cut(s) 775
Csp6I GTAC 2 cut(s) 379, 937
CviQI GTAC 2 cut(s) 379, 937
DdeI CTNAG 2 cut(s) 258, 344
DpnI GATC 7 cut(s) 23, 497, 501, 675, 795, 978, 1007
DpnII GATC 7 cut(s) 21, 495, 499, 673, 793, 976, 1005
EaeI YGGCCR 2 cut(s) 604, 967
Eco130I CCWWGG 5 cut(s) 220, 338, 399, 583, 681
Eco147I AGGCCT 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 600
Eco31I GGTCTC 1 cut(s) 989
Eco47I GGWCC 3 cut(s) 334, 403, 578
Eco57I CTGAAG 2 cut(s) 540, 916
Eco88I CYCGRG 1 cut(s) 197
Eco91I GGTNACC 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 578
EcoO65I GGTNACC 1 cut(s) 179
EcoT14I CCWWGG 5 cut(s) 220, 338, 399, 583, 681
EcoT38I GRGCYC 1 cut(s) 600
ErhI CCWWGG 5 cut(s) 220, 338, 399, 583, 681
FalI AAGNNNNNCTT 2 cut(s) 857, 889
FaqI GGGAC 4 cut(s) 320, 389, 494, 773
Fnu4HI GCNGC 5 cut(s) 474, 720, 754, 951, 970
FokI GGATG 2 cut(s) 410, 724
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 5 cut(s) 474, 720, 754, 951, 970
GluI GCNGC 5 cut(s) 474, 720, 754, 951, 970
GsaI CCCAGC 2 cut(s) 515, 597
HaeIII GGCC 7 cut(s) 105, 225, 349, 398, 606, 886, 969
HapII CCGG 4 cut(s) 607, 741, 839, 887
HgaI GACGC 1 cut(s) 775
Hin1I GRCGYC 1 cut(s) 786
HincII GTYRAC 2 cut(s) 136, 688
HindII GTYRAC 2 cut(s) 136, 688
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 1 cut(s) 564
HpaII CCGG 4 cut(s) 607, 741, 839, 887
HphI GGTGA 4 cut(s) 29, 173, 178, 265
Hpy166II GTNNAC 4 cut(s) 87, 136, 418, 688
Hpy188I TCNGA 2 cut(s) 62, 320
Hpy188III TCNNGA 3 cut(s) 666, 980, 1003
Hpy8I GTNNAC 4 cut(s) 87, 136, 418, 688
HpyAV CCTTC 2 cut(s) 478, 564
HpyCH4III ACNGT 4 cut(s) 334, 373, 454, 692
HpyCH4V TGCA 7 cut(s) 72, 123, 164, 418, 701, 737, 779
HpyF10VI GCNNNNNNNGC 1 cut(s) 222
HpyF3I CTNAG 2 cut(s) 258, 344
Hsp92I GRCGYC 1 cut(s) 786
KpnI GGTACC 1 cut(s) 940
Kzo9I GATC 7 cut(s) 21, 495, 499, 673, 793, 976, 1005
LmnI GCTCC 1 cut(s) 209
Lsp1109I GCAGC 3 cut(s) 460, 731, 937
LweI GCATC 3 cut(s) 100, 580, 746
MaeIII GTNAC 3 cut(s) 179, 367, 454
MalI GATC 7 cut(s) 23, 497, 501, 675, 795, 978, 1007
MboI GATC 7 cut(s) 21, 495, 499, 673, 793, 976, 1005
MboII GAAGA 2 cut(s) 4, 622
MfeI CAATTG 1 cut(s) 780
MflI RGATCY 3 cut(s) 673, 793, 1005
MhlI GDGCHC 3 cut(s) 420, 600, 646
MluCI AATT 6 cut(s) 78, 159, 204, 518, 615, 780
MmeI TCCRAC 1 cut(s) 701
MseI TTAA 1 cut(s) 1024
MslI CAYNNNNRTG 2 cut(s) 588, 651
MspA1I CMGCKG 2 cut(s) 95, 756
MspI CCGG 4 cut(s) 607, 741, 839, 887
MspR9I CCNGG 3 cut(s) 742, 839, 888
MunI CAATTG 1 cut(s) 780
MvnI CGCG 1 cut(s) 972
MwoI GCNNNNNNNGC 1 cut(s) 222
NciI CCSGG 3 cut(s) 742, 839, 888
NcoI CCATGG 3 cut(s) 338, 399, 583
NdeII GATC 7 cut(s) 21, 495, 499, 673, 793, 976, 1005
NlaIV GGNNCC 6 cut(s) 301, 336, 397, 405, 580, 938
NmuCI GTSAC 3 cut(s) 179, 367, 454
NspI RCATGY 2 cut(s) 232, 494
PceI AGGCCT 1 cut(s) 105
PciI ACATGT 2 cut(s) 228, 490
PcsI WCGNNNNNNNCGW 1 cut(s) 504
PfeI GAWTC 1 cut(s) 564
PfoI TCCNGGA 1 cut(s) 837
PkrI GCNGC 5 cut(s) 475, 721, 755, 952, 971
PpuMI RGGWCCY 1 cut(s) 578
PscI ACATGT 2 cut(s) 228, 490
Psp5II RGGWCCY 1 cut(s) 578
PspEI GGTNACC 1 cut(s) 179
PspFI CCCAGC 2 cut(s) 511, 593
PspN4I GGNNCC 6 cut(s) 301, 336, 397, 405, 580, 938
PspPI GGNCC 5 cut(s) 334, 348, 396, 403, 578
PspPPI RGGWCCY 1 cut(s) 578
PsuI RGATCY 3 cut(s) 673, 793, 1005
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 2 cut(s) 380, 938
RsaNI GTAC 2 cut(s) 379, 937
RseI CAYNNNNRTG 2 cut(s) 588, 651
SaqAI TTAA 1 cut(s) 1024
SatI GCNGC 5 cut(s) 474, 720, 754, 951, 970
Sau3AI GATC 7 cut(s) 21, 495, 499, 673, 793, 976, 1005
Sau96I GGNCC 5 cut(s) 334, 348, 396, 403, 578
ScrFI CCNGG 3 cut(s) 742, 839, 888
SduI GDGCHC 3 cut(s) 420, 600, 646
SfaNI GCATC 3 cut(s) 100, 580, 746
SinI GGWCC 3 cut(s) 334, 403, 578
SmiMI CAYNNNNRTG 2 cut(s) 588, 651
SmlI CTYRAG 1 cut(s) 1010
SmoI CTYRAG 1 cut(s) 1010
Sse9I AATT 6 cut(s) 78, 159, 204, 518, 615, 780
SseBI AGGCCT 1 cut(s) 105
SsiI CCGC 2 cut(s) 754, 970
StuI AGGCCT 1 cut(s) 105
StyD4I CCNGG 3 cut(s) 740, 837, 886
StyI CCWWGG 5 cut(s) 220, 338, 399, 583, 681
TaaI ACNGT 4 cut(s) 334, 373, 454, 692
TaqI TCGA 2 cut(s) 498, 562
TasI AATT 6 cut(s) 78, 159, 204, 518, 615, 780
TauI GCSGC 2 cut(s) 756, 972
TfiI GAWTC 1 cut(s) 564
Tru1I TTAA 1 cut(s) 1024
Tru9I TTAA 1 cut(s) 1024
TscAI CASTG 2 cut(s) 250, 607
TseFI GTSAC 3 cut(s) 179, 367, 454
TseI GCWGC 3 cut(s) 473, 719, 950
Tsp45I GTSAC 3 cut(s) 179, 367, 454
TspDTI ATGAA 3 cut(s) 164, 253, 511
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 2 cut(s) 250, 607
VneI GTGCAC 1 cut(s) 416
VpaK11BI GGWCC 3 cut(s) 334, 403, 578
XceI RCATGY 2 cut(s) 232, 494
XcmI CCANNNNNNNNNTGG 2 cut(s) 590, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.