pycom04g12200

Protein of unknown function, DUF604

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0006722
Physical Location & Seq
Reverse (-)
15185168 .. 15186719
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g12200.1

Sequence Viewer

Length: 834 bp
ATGTACTATATTGGCGGGAACTCGGAGAGTGTGGAGCAAGATGTGATCCACTCATACACTATGGCTTACGGCGGTGGCGGGTTTGCCATTAGTTACCCGTTGGCTGCTGTGCTTGTTAAAATTTTGGACGGCTGCATTGATCGGTATGATAAACTCTACGGCTCGGATCAAAAGATCCAGGGTTGCTTGAGCGAGATTGGAGTGCCGCTAACAAAAGAGCTCGGCTTTCACCAACTTGATATACGGGGCAACCCGTATGGTTTGCTAGCGGCGCACCCTCTGACCCCGTTGGTGTCACTCCACCATTTGGACTATGTGGAGTCGATATTTCCAAACCTGAACCCGATTGACTCGGTCAAGAAGTTGGTTAGCGGGTACAAAGTGGATCCGGGCCGGACCGTACAACATAGTTTTTGTTACGACGTGAGGCGTAATTGGTCTGTTTCGGTGTCTTGGGGCTACACGGTGCAGTTGTATCCGTTTTTTGTGACGGCTAAGAAGCTGGAAACCGCGTTGCAGACGTTTTGGACGTGGAAGAACTGGGACGCGCGCCTATTCACGTTTAATACCCAACCCGTCAGTTCGGACCCGTGTGAGAGATCGGTTGTGTACATGTTAGATCGGGTTGAAAGTGTTGGTGGGGGTGAGACCCTGACTACTTACAAGAGGTACAATATTGACAAAGGGGAAAACGATTGCATCCGAAGTGACTATACTCATGCTTTGAAAGTTGAGGCTTTCAATGTCTCAGCTCCCAAATTCAACCCAGACTTGTGGAATAAGGCGCCACGTAGACAGTGTTGCGAGATCGATCAATGGCACAAAAGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF604

Protein Analysis

278

Amino Acids

31.52

Weight (kDa)

6.7

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF604 PF04646 13 - 268 2.2e-106 Protein of unknown function, DUF604
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 784
AccB7I CCANNNNNTGG 1 cut(s) 307
AccI GTMKAC 1 cut(s) 793
AccII CGCG 3 cut(s) 512, 548, 550
AciI CCGC 7 cut(s) 15, 72, 78, 206, 269, 372, 510
AclWI GGATC 5 cut(s) 40, 169, 174, 380, 393
AcsI RAATTY 2 cut(s) 120, 758
AcyI GRCGYC 1 cut(s) 785
AfaI GTAC 5 cut(s) 5, 377, 402, 611, 671
AfiI CCNNNNNNNGG 1 cut(s) 307
AflIII ACRYGT 1 cut(s) 612
AgsI TTSAA 4 cut(s) 629, 727, 742, 763
AjiI CACGTC 2 cut(s) 424, 531
AjnI CCWGG 1 cut(s) 177
AluBI AGCT 4 cut(s) 220, 502, 752, 831
AluI AGCT 4 cut(s) 220, 502, 752, 831
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 2 cut(s) 641, 751
AlwI GGATC 5 cut(s) 40, 169, 174, 380, 393
AoxI GGCC 1 cut(s) 391
ApeKI GCWGC 2 cut(s) 104, 132
ApoI RAATTY 2 cut(s) 120, 758
AspLEI GCGC 4 cut(s) 274, 550, 552, 787
AspS9I GGNCC 3 cut(s) 391, 396, 586
AsuC2I CCSGG 1 cut(s) 390
AsuHPI GGTGA 2 cut(s) 221, 656
AsuNHI GCTAGC 1 cut(s) 265
AvaII GGWCC 2 cut(s) 396, 586
BamHI GGATCC 1 cut(s) 385
BanI GGYRCC 1 cut(s) 784
BanII GRGCYC 1 cut(s) 222
BarI GAAGNNNNNNTAC 2 cut(s) 697, 729
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 2 cut(s) 91, 119
BceAI ACGGC 4 cut(s) 85, 145, 175, 507
BciT130I CCWGG 1 cut(s) 179
BciVI GTATCC 1 cut(s) 486
BcnI CCSGG 1 cut(s) 390
BcoDI GTCTC 2 cut(s) 641, 751
BfaI CTAG 1 cut(s) 266
BfoI RGCGCY 1 cut(s) 788
BfuI GTATCC 1 cut(s) 486
BisI GCNGC 4 cut(s) 105, 133, 206, 270
BlsI GCNGC 4 cut(s) 106, 134, 207, 271
Bme1390I CCNGG 2 cut(s) 179, 390
Bme18I GGWCC 2 cut(s) 396, 586
BmgBI CACGTC 2 cut(s) 424, 531
BmgT120I GGNCC 3 cut(s) 391, 396, 586
BmiI GGNNCC 3 cut(s) 387, 588, 786
BmrFI CCNGG 2 cut(s) 179, 390
BmrI ACTGGG 1 cut(s) 550
BmsI GCATC 1 cut(s) 708
BmtI GCTAGC 1 cut(s) 269
BmuI ACTGGG 1 cut(s) 550
BpuEI CTTGAG 1 cut(s) 208
BpuMI CCSGG 1 cut(s) 390
Bsa29I ATCGAT 1 cut(s) 810
BsaAI YACGTR 1 cut(s) 791
BsaHI GRCGYC 1 cut(s) 785
BsaI GGTCTC 1 cut(s) 641
BsaJI CCNNGG 1 cut(s) 178
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 1 cut(s) 545
BseBI CCWGG 1 cut(s) 179
BseCI ATCGAT 1 cut(s) 810
BseDI CCNNGG 1 cut(s) 178
BseGI GGATG 1 cut(s) 699
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 762
BseNI ACTGG 1 cut(s) 545
BsePI GCGCGC 1 cut(s) 548
BseXI GCAGC 2 cut(s) 91, 119
BsgI GTGCAG 1 cut(s) 488
Bsh1236I CGCG 3 cut(s) 512, 548, 550
BshFI GGCC 1 cut(s) 393
BshNI GGYRCC 1 cut(s) 784
BshVI ATCGAT 1 cut(s) 810
BsiHKAI GWGCWC 1 cut(s) 222
BsiSI CCGG 2 cut(s) 389, 394
BslFI GGGAC 1 cut(s) 557
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 2 cut(s) 641, 751
BsmFI GGGAC 1 cut(s) 557
BsnI GGCC 1 cut(s) 393
Bso31I GGTCTC 1 cut(s) 641
Bsp1286I GDGCHC 1 cut(s) 222
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 9 cut(s) 45, 139, 166, 174, 385, 599, 619, 807, 811
BspACI CCGC 7 cut(s) 15, 72, 78, 206, 269, 372, 510
BspANI GGCC 1 cut(s) 393
BspCNI CTCAG 1 cut(s) 761
BspDI ATCGAT 1 cut(s) 810
BspFNI CGCG 3 cut(s) 512, 548, 550
BspLI GGNNCC 3 cut(s) 387, 588, 786
BspOI GCTAGC 1 cut(s) 269
BspPI GGATC 5 cut(s) 40, 169, 174, 380, 393
BspT107I GGYRCC 1 cut(s) 784
BspTNI GGTCTC 1 cut(s) 641
BsrGI TGTACA 1 cut(s) 609
BsrI ACTGG 1 cut(s) 545
BssECI CCNNGG 1 cut(s) 178
BssHII GCGCGC 1 cut(s) 548
BssMI GATC 9 cut(s) 45, 139, 166, 174, 385, 599, 619, 807, 811
BssNI GRCGYC 1 cut(s) 785
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 3 cut(s) 400, 466, 798
BstACI GRCGYC 1 cut(s) 785
BstAUI TGTACA 1 cut(s) 609
BstBAI YACGTR 1 cut(s) 791
BstC8I GCNNGC 2 cut(s) 267, 550
BstDEI CTNAG 2 cut(s) 495, 748
BstF5I GGATG 1 cut(s) 699
BstFNI CGCG 3 cut(s) 512, 548, 550
BstH2I RGCGCY 1 cut(s) 788
BstHHI GCGC 4 cut(s) 274, 550, 552, 787
BstKTI GATC 9 cut(s) 48, 142, 169, 177, 388, 602, 622, 810, 814
BstMAI GTCTC 2 cut(s) 641, 751
BstMBI GATC 9 cut(s) 45, 139, 166, 174, 385, 599, 619, 807, 811
BstMWI GCNNNNNNNGC 1 cut(s) 271
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 2 cut(s) 177, 388
BstUI CGCG 3 cut(s) 512, 548, 550
BstV1I GCAGC 2 cut(s) 91, 119
BstX2I RGATCY 2 cut(s) 174, 385
BstXI CCANNNNNNTGG 1 cut(s) 774
BstYI RGATCY 2 cut(s) 174, 385
Bsu15I ATCGAT 1 cut(s) 810
BsuI GTATCC 1 cut(s) 486
BsuRI GGCC 1 cut(s) 393
BsuTUI ATCGAT 1 cut(s) 810
BtrI CACGTC 2 cut(s) 424, 531
BtsCI GGATG 1 cut(s) 699
BtsIMutI CAGTG 1 cut(s) 803
Cac8I GCNNGC 2 cut(s) 267, 550
CfoI GCGC 4 cut(s) 274, 550, 552, 787
Cfr13I GGNCC 3 cut(s) 391, 396, 586
ClaI ATCGAT 1 cut(s) 810
CpoI CGGWCCG 1 cut(s) 396
CseI GACGC 1 cut(s) 554
Csp6I GTAC 5 cut(s) 4, 376, 401, 610, 670
CspI CGGWCCG 1 cut(s) 396
CviAII CATG 2 cut(s) 613, 719
CviQI GTAC 5 cut(s) 4, 376, 401, 610, 670
DdeI CTNAG 2 cut(s) 495, 748
DinI GGCGCC 1 cut(s) 786
DpnI GATC 9 cut(s) 47, 141, 168, 176, 387, 601, 621, 809, 813
DpnII GATC 9 cut(s) 45, 139, 166, 174, 385, 599, 619, 807, 811
Ecl136II GAGCTC 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 222
Eco31I GGTCTC 1 cut(s) 641
Eco47I GGWCC 2 cut(s) 396, 586
Eco53kI GAGCTC 1 cut(s) 220
EcoICRI GAGCTC 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 177
EcoT38I GRGCYC 1 cut(s) 222
EgeI GGCGCC 1 cut(s) 786
EheI GGCGCC 1 cut(s) 786
FaeI CATG 2 cut(s) 616, 722
FaqI GGGAC 1 cut(s) 557
FatI CATG 2 cut(s) 612, 718
FauI CCCGC 3 cut(s) 8, 71, 365
FblI GTMKAC 1 cut(s) 793
Fnu4HI GCNGC 4 cut(s) 105, 133, 206, 270
FokI GGATG 1 cut(s) 686
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 4 cut(s) 105, 133, 206, 270
FspBI CTAG 1 cut(s) 266
GlaI GCGC 4 cut(s) 273, 549, 551, 786
GluI GCNGC 4 cut(s) 105, 133, 206, 270
HaeII RGCGCY 1 cut(s) 788
HaeIII GGCC 1 cut(s) 393
HapII CCGG 2 cut(s) 389, 394
HgaI GACGC 1 cut(s) 554
HhaI GCGC 4 cut(s) 274, 550, 552, 787
Hin1I GRCGYC 1 cut(s) 785
Hin1II CATG 2 cut(s) 616, 722
Hin6I GCGC 4 cut(s) 272, 548, 550, 785
HinP1I GCGC 4 cut(s) 272, 548, 550, 785
HinfI GANTC 2 cut(s) 320, 350
HpaII CCGG 2 cut(s) 389, 394
HphI GGTGA 2 cut(s) 221, 656
Hpy166II GTNNAC 2 cut(s) 610, 794
Hpy188I TCNGA 5 cut(s) 25, 166, 282, 586, 704
Hpy188III TCNNGA 1 cut(s) 358
Hpy8I GTNNAC 2 cut(s) 610, 794
Hpy99I CGWCG 1 cut(s) 425
HpyCH4III ACNGT 3 cut(s) 400, 466, 798
HpyCH4IV ACGT 5 cut(s) 423, 521, 530, 560, 790
HpyCH4V TGCA 4 cut(s) 135, 469, 517, 699
HpyF10VI GCNNNNNNNGC 1 cut(s) 271
HpyF3I CTNAG 2 cut(s) 495, 748
HpySE526I ACGT 5 cut(s) 423, 521, 530, 560, 790
Hsp92I GRCGYC 1 cut(s) 785
Hsp92II CATG 2 cut(s) 616, 722
HspAI GCGC 4 cut(s) 272, 548, 550, 785
KasI GGCGCC 1 cut(s) 784
Kzo9I GATC 9 cut(s) 45, 139, 166, 174, 385, 599, 619, 807, 811
LmnI GCTCC 3 cut(s) 34, 757, 828
LpnPI CCDG 9 cut(s) 164, 191, 350, 402, 407, 488, 526, 665, 780
Lsp1109I GCAGC 2 cut(s) 91, 119
LweI GCATC 1 cut(s) 708
MaeI CTAG 1 cut(s) 266
MaeII ACGT 5 cut(s) 423, 521, 530, 560, 790
MaeIII GTNAC 5 cut(s) 92, 294, 416, 487, 707
MalI GATC 9 cut(s) 47, 141, 168, 176, 387, 601, 621, 809, 813
MboI GATC 9 cut(s) 45, 139, 166, 174, 385, 599, 619, 807, 811
MboII GAAGA 1 cut(s) 547
MflI RGATCY 2 cut(s) 174, 385
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 3 cut(s) 120, 433, 758
Mly113I GGCGCC 1 cut(s) 785
MlyI GAGTC 2 cut(s) 329, 344
MnlI CCTC 4 cut(s) 288, 420, 660, 727
MseI TTAA 2 cut(s) 117, 564
MspI CCGG 2 cut(s) 389, 394
MspR9I CCNGG 2 cut(s) 179, 390
MvaI CCWGG 1 cut(s) 179
MvnI CGCG 3 cut(s) 512, 548, 550
MwoI GCNNNNNNNGC 1 cut(s) 271
NarI GGCGCC 1 cut(s) 785
NciI CCSGG 1 cut(s) 390
NdeII GATC 9 cut(s) 45, 139, 166, 174, 385, 599, 619, 807, 811
NheI GCTAGC 1 cut(s) 265
NlaIII CATG 2 cut(s) 616, 722
NlaIV GGNNCC 3 cut(s) 387, 588, 786
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 3 cut(s) 294, 487, 707
NspI RCATGY 1 cut(s) 616
PauI GCGCGC 1 cut(s) 548
PciI ACATGT 1 cut(s) 612
PcsI WCGNNNNNNNCGW 1 cut(s) 527
PflFI GACNNNGTC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 307
PkrI GCNGC 4 cut(s) 106, 134, 207, 271
PleI GAGTC 2 cut(s) 328, 344
PluTI GGCGCC 1 cut(s) 788
PpsI GAGTC 2 cut(s) 328, 344
Ppu21I YACGTR 1 cut(s) 791
PscI ACATGT 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 3 cut(s) 387, 588, 786
PspPI GGNCC 3 cut(s) 391, 396, 586
PsuI RGATCY 2 cut(s) 174, 385
PsyI GACNNNGTC 1 cut(s) 353
PteI GCGCGC 1 cut(s) 548
RsaI GTAC 5 cut(s) 5, 377, 402, 611, 671
RsaNI GTAC 5 cut(s) 4, 376, 401, 610, 670
Rsr2I CGGWCCG 1 cut(s) 396
RsrII CGGWCCG 1 cut(s) 396
SacI GAGCTC 1 cut(s) 222
SaqAI TTAA 2 cut(s) 117, 564
SatI GCNGC 4 cut(s) 105, 133, 206, 270
Sau3AI GATC 9 cut(s) 45, 139, 166, 174, 385, 599, 619, 807, 811
Sau96I GGNCC 3 cut(s) 391, 396, 586
SchI GAGTC 2 cut(s) 329, 344
ScrFI CCNGG 2 cut(s) 179, 390
SduI GDGCHC 1 cut(s) 222
SfaNI GCATC 1 cut(s) 708
SfoI GGCGCC 1 cut(s) 786
SinI GGWCC 2 cut(s) 396, 586
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
Sse9I AATT 3 cut(s) 120, 433, 758
SsiI CCGC 7 cut(s) 15, 72, 78, 206, 269, 372, 510
SspDI GGCGCC 1 cut(s) 784
SspI AATATT 1 cut(s) 676
SspMI CTAG 1 cut(s) 266
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 2 cut(s) 177, 388
TaaI ACNGT 3 cut(s) 400, 466, 798
TaiI ACGT 5 cut(s) 426, 524, 533, 563, 793
TaqI TCGA 2 cut(s) 323, 810
TaqII GACCGA 1 cut(s) 343
TasI AATT 3 cut(s) 120, 433, 758
TatI WGTACW 2 cut(s) 3, 609
TauI GCSGC 2 cut(s) 208, 272
Tru1I TTAA 2 cut(s) 117, 564
Tru9I TTAA 2 cut(s) 117, 564
TscAI CASTG 1 cut(s) 803
TseFI GTSAC 3 cut(s) 294, 487, 707
TseI GCWGC 2 cut(s) 104, 132
Tsp45I GTSAC 3 cut(s) 294, 487, 707
TspGWI ACGGA 1 cut(s) 468
TspRI CASTG 1 cut(s) 803
Tth111I GACNNNGTC 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 307
VpaK11BI GGWCC 2 cut(s) 396, 586
XapI RAATTY 2 cut(s) 120, 758
XceI RCATGY 1 cut(s) 616
XmiI GTMKAC 1 cut(s) 793
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.