Rorug03G0077700

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000911
Physical Location & Seq
Forward (+)
6080391 .. 6081954
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0077700.1

Sequence Viewer

Length: 573 bp
ATGGCAAACGTAGAACCAACCACAGCCGGAGACCAAGACGCCGCCAAAACACCTCACAGGTACGTAGCGTGGGGCCTTCTGCCCGATCCGGTTTCCCGTCGGATCAAAAACGTGACGGAGGGCCTCCGGGCCGAGTTCGGTGGGCCGGAGATCATCGTGCCCCACATACCCATTTTAGGGTCCATCATTTTGACGGAGGAAGACGCCGTCAACACCTTCAGACAAGCCTGCCAGGCCATCGGTACCATCCCTTGCAAGGTCACTCACGTCGCCACCAGTCCCTTCTATTACCAGTGTGTTCACCTCTTCATTGATCCAGACCATGAGGTGCAGCAACGAACTGAAACTTTCGCGCGCTTTTTCAGACGCATTAGTAATATGCTACATCTGAGTCTCCTTTATGGGGAGCTGACAGATGAAGAGAAGAAACGAGCTCAAGAAAAAGTTAGTGTTCTGGATGAGGCCATTACCAGCTTCAATTTCACCATAGATCGCCTTGCATTGTACAAATGTCACGATGAAGACAGAACTCAACAGTCTTGGGAGAAGATTCTTGAACTCCGCCTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

190

Amino Acids

21.75

Weight (kDa)

5.74

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 242
AccII CGCG 2 cut(s) 353, 355
AciI CCGC 2 cut(s) 42, 562
AclWI GGATC 3 cut(s) 80, 110, 308
AcuI CTGAAG 1 cut(s) 202
AcyI GRCGYC 2 cut(s) 39, 204
AfaI GTAC 3 cut(s) 62, 244, 506
AfiI CCNNNNNNNGG 4 cut(s) 176, 177, 256, 403
AgsI TTSAA 2 cut(s) 478, 557
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 1 cut(s) 231
AluBI AGCT 3 cut(s) 409, 434, 474
AluI AGCT 3 cut(s) 409, 434, 474
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 2 cut(s) 24, 398
AlwI GGATC 3 cut(s) 80, 110, 308
AoxI GGCC 6 cut(s) 73, 121, 129, 143, 234, 462
ApeKI GCWGC 1 cut(s) 331
Asp718I GGTACC 1 cut(s) 242
AspLEI GCGC 2 cut(s) 355, 357
AspS9I GGNCC 5 cut(s) 73, 121, 129, 143, 180
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 2 cut(s) 293, 475
AvaII GGWCC 1 cut(s) 180
BaeGI GKGCMC 1 cut(s) 162
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 1 cut(s) 436
BbsI GAAGAC 2 cut(s) 207, 528
Bbv12I GWGCWC 1 cut(s) 436
BbvI GCAGC 1 cut(s) 343
BccI CCATC 3 cut(s) 191, 245, 254
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 1 cut(s) 233
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 2 cut(s) 24, 398
BglI GCCNNNNNGGC 1 cut(s) 233
BisI GCNGC 2 cut(s) 42, 332
BlsI GCNGC 2 cut(s) 43, 333
Bme1390I CCNGG 2 cut(s) 128, 233
Bme18I GGWCC 1 cut(s) 180
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 5 cut(s) 73, 121, 129, 143, 180
BmiI GGNNCC 3 cut(s) 74, 181, 244
BmrFI CCNGG 2 cut(s) 128, 233
BpiI GAAGAC 2 cut(s) 207, 528
BpuEI CTTGAG 1 cut(s) 420
BpuMI CCSGG 1 cut(s) 128
BsaAI YACGTR 1 cut(s) 64
BsaHI GRCGYC 2 cut(s) 39, 204
BsaI GGTCTC 1 cut(s) 24
BsaWI WCCGGW 1 cut(s) 88
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 177, 256, 403
Bse1I ACTGG 2 cut(s) 276, 292
BseBI CCWGG 1 cut(s) 233
BseGI GGATG 2 cut(s) 246, 463
BseLI CCNNNNNNNGG 4 cut(s) 176, 177, 256, 403
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 2 cut(s) 276, 292
BsePI GCGCGC 1 cut(s) 353
BseSI GKGCMC 1 cut(s) 162
BseXI GCAGC 1 cut(s) 343
BsgI GTGCAG 1 cut(s) 350
Bsh1236I CGCG 2 cut(s) 353, 355
BshFI GGCC 6 cut(s) 75, 123, 131, 145, 236, 464
BshNI GGYRCC 1 cut(s) 242
BsiHKAI GWGCWC 1 cut(s) 436
BsiSI CCGG 4 cut(s) 27, 89, 127, 146
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 4 cut(s) 176, 177, 256, 403
BsmAI GTCTC 2 cut(s) 24, 398
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 6 cut(s) 75, 123, 131, 145, 236, 464
Bso31I GGTCTC 1 cut(s) 24
Bsp1286I GDGCHC 2 cut(s) 162, 436
Bsp1407I TGTACA 1 cut(s) 504
Bsp143I GATC 5 cut(s) 85, 102, 150, 313, 490
BspACI CCGC 2 cut(s) 42, 562
BspANI GGCC 6 cut(s) 75, 123, 131, 145, 236, 464
BspCNI CTCAG 1 cut(s) 381
BspFNI CGCG 2 cut(s) 353, 355
BspLI GGNNCC 3 cut(s) 74, 181, 244
BspPI GGATC 3 cut(s) 80, 110, 308
BspT107I GGYRCC 1 cut(s) 242
BspTNI GGTCTC 1 cut(s) 24
BsrGI TGTACA 1 cut(s) 504
BsrI ACTGG 2 cut(s) 276, 292
BssHII GCGCGC 1 cut(s) 353
BssMI GATC 5 cut(s) 85, 102, 150, 313, 490
BssNI GRCGYC 2 cut(s) 39, 204
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 1 cut(s) 537
Bst6I CTCTTC 2 cut(s) 311, 414
BstACI GRCGYC 2 cut(s) 39, 204
BstAUI TGTACA 1 cut(s) 504
BstBAI YACGTR 1 cut(s) 64
BstC8I GCNNGC 2 cut(s) 229, 355
BstDEI CTNAG 1 cut(s) 389
BstF5I GGATG 2 cut(s) 246, 463
BstFNI CGCG 2 cut(s) 353, 355
BstHHI GCGC 2 cut(s) 355, 357
BstKTI GATC 5 cut(s) 88, 105, 153, 316, 493
BstMAI GTCTC 2 cut(s) 24, 398
BstMBI GATC 5 cut(s) 85, 102, 150, 313, 490
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstNI CCWGG 1 cut(s) 233
BstSCI CCNGG 2 cut(s) 126, 231
BstSLI GKGCMC 1 cut(s) 162
BstSNI TACGTA 1 cut(s) 64
BstUI CGCG 2 cut(s) 353, 355
BstV1I GCAGC 1 cut(s) 343
BstV2I GAAGAC 2 cut(s) 207, 528
BsuRI GGCC 6 cut(s) 75, 123, 131, 145, 236, 464
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 2 cut(s) 246, 463
BtsIMutI CAGTG 1 cut(s) 299
Cac8I GCNNGC 2 cut(s) 229, 355
CfoI GCGC 2 cut(s) 355, 357
Cfr13I GGNCC 5 cut(s) 73, 121, 129, 143, 180
CseI GACGC 3 cut(s) 47, 212, 375
Csp6I GTAC 3 cut(s) 61, 243, 505
CviAII CATG 1 cut(s) 323
CviQI GTAC 3 cut(s) 61, 243, 505
DdeI CTNAG 1 cut(s) 389
DpnI GATC 5 cut(s) 87, 104, 152, 315, 492
DpnII GATC 5 cut(s) 85, 102, 150, 313, 490
Eam1104I CTCTTC 2 cut(s) 311, 414
EarI CTCTTC 2 cut(s) 311, 414
EciI GGCGGA 1 cut(s) 551
Ecl136II GAGCTC 1 cut(s) 434
Eco105I TACGTA 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 436
Eco31I GGTCTC 1 cut(s) 24
Eco47I GGWCC 1 cut(s) 180
Eco53kI GAGCTC 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 202
EcoICRI GAGCTC 1 cut(s) 434
EcoO109I RGGNCCY 2 cut(s) 73, 121
EcoRII CCWGG 1 cut(s) 231
EcoT38I GRGCYC 1 cut(s) 436
FaeI CATG 1 cut(s) 326
FaiI YATR 5 cut(s) 167, 324, 380, 402, 488
FaqI GGGAC 1 cut(s) 264
FatI CATG 1 cut(s) 322
Fnu4HI GCNGC 2 cut(s) 42, 332
FokI GGATG 2 cut(s) 233, 470
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 2 cut(s) 42, 332
GlaI GCGC 2 cut(s) 354, 356
GluI GCNGC 2 cut(s) 42, 332
HaeIII GGCC 6 cut(s) 75, 123, 131, 145, 236, 464
HapII CCGG 4 cut(s) 27, 89, 127, 146
HgaI GACGC 3 cut(s) 47, 212, 375
HhaI GCGC 2 cut(s) 355, 357
Hin1I GRCGYC 2 cut(s) 39, 204
Hin1II CATG 1 cut(s) 326
Hin6I GCGC 2 cut(s) 353, 355
HinP1I GCGC 2 cut(s) 353, 355
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 2 cut(s) 391, 550
HpaII CCGG 4 cut(s) 27, 89, 127, 146
HphI GGTGA 2 cut(s) 293, 475
Hpy166II GTNNAC 2 cut(s) 211, 301
Hpy188I TCNGA 4 cut(s) 102, 221, 365, 390
Hpy188III TCNNGA 5 cut(s) 317, 437, 455, 515, 554
Hpy8I GTNNAC 2 cut(s) 211, 301
Hpy99I CGWCG 2 cut(s) 102, 272
HpyAV CCTTC 3 cut(s) 86, 226, 292
HpyCH4III ACNGT 1 cut(s) 537
HpyCH4IV ACGT 4 cut(s) 9, 63, 111, 267
HpyCH4V TGCA 3 cut(s) 255, 331, 500
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 1 cut(s) 389
HpySE526I ACGT 4 cut(s) 9, 63, 111, 267
Hsp92I GRCGYC 2 cut(s) 39, 204
Hsp92II CATG 1 cut(s) 326
HspAI GCGC 2 cut(s) 353, 355
KpnI GGTACC 1 cut(s) 246
Kzo9I GATC 5 cut(s) 85, 102, 150, 313, 490
LmnI GCTCC 1 cut(s) 406
Lsp1109I GCAGC 1 cut(s) 343
MaeII ACGT 4 cut(s) 9, 63, 111, 267
MaeIII GTNAC 3 cut(s) 112, 259, 512
MalI GATC 5 cut(s) 87, 104, 152, 315, 492
MboI GATC 5 cut(s) 85, 102, 150, 313, 490
MboII GAAGA 6 cut(s) 212, 298, 431, 436, 533, 559
MhlI GDGCHC 2 cut(s) 162, 436
MluCI AATT 1 cut(s) 478
MlyI GAGTC 1 cut(s) 400
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 7 cut(s) 63, 112, 134, 190, 314, 319, 454
MspI CCGG 4 cut(s) 27, 89, 127, 146
MspR9I CCNGG 2 cut(s) 128, 233
MvaI CCWGG 1 cut(s) 233
MvnI CGCG 2 cut(s) 353, 355
MwoI GCNNNNNNNGC 1 cut(s) 233
NciI CCSGG 1 cut(s) 128
NdeII GATC 5 cut(s) 85, 102, 150, 313, 490
NlaIII CATG 1 cut(s) 326
NlaIV GGNNCC 3 cut(s) 74, 181, 244
NmeAIII GCCGAG 1 cut(s) 157
NmuCI GTSAC 3 cut(s) 112, 259, 512
PauI GCGCGC 1 cut(s) 353
PfeI GAWTC 1 cut(s) 550
PflFI GACNNNGTC 1 cut(s) 206
PkrI GCNGC 2 cut(s) 43, 333
PleI GAGTC 1 cut(s) 399
PpsI GAGTC 1 cut(s) 399
Ppu21I YACGTR 1 cut(s) 64
Psp124BI GAGCTC 1 cut(s) 436
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 3 cut(s) 74, 181, 244
PspPI GGNCC 5 cut(s) 73, 121, 129, 143, 180
PsyI GACNNNGTC 1 cut(s) 206
PteI GCGCGC 1 cut(s) 353
RsaI GTAC 3 cut(s) 62, 244, 506
RsaNI GTAC 3 cut(s) 61, 243, 505
SacI GAGCTC 1 cut(s) 436
SatI GCNGC 2 cut(s) 42, 332
Sau3AI GATC 5 cut(s) 85, 102, 150, 313, 490
Sau96I GGNCC 5 cut(s) 73, 121, 129, 143, 180
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 2 cut(s) 128, 233
SduI GDGCHC 2 cut(s) 162, 436
SinI GGWCC 1 cut(s) 180
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
SnaBI TACGTA 1 cut(s) 64
Sse9I AATT 1 cut(s) 478
SsiI CCGC 2 cut(s) 42, 562
SstI GAGCTC 1 cut(s) 436
StyD4I CCNGG 2 cut(s) 126, 231
TaaI ACNGT 1 cut(s) 537
TaiI ACGT 4 cut(s) 12, 66, 114, 270
TasI AATT 1 cut(s) 478
TatI WGTACW 1 cut(s) 504
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 1 cut(s) 550
TscAI CASTG 1 cut(s) 299
TseFI GTSAC 3 cut(s) 112, 259, 512
TseI GCWGC 1 cut(s) 331
Tsp45I GTSAC 3 cut(s) 112, 259, 512
TspDTI ATGAA 4 cut(s) 298, 432, 534, 557
TspGWI ACGGA 2 cut(s) 131, 209
TspRI CASTG 1 cut(s) 299
Tth111I GACNNNGTC 1 cut(s) 206
VpaK11BI GGWCC 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.