RLG00000035850

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000911
Physical Location & Seq
Reverse (-)
72591113 .. 72593763
2651 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035850

Sequence Viewer

Length: 1431 bp
ATGGCTTCCACAGTCTCAACTGTTGGAGCTGTTAACAGAACACCCTTGAGCTTGAACAGCTCTGGTGCTGGAAGCTCAATCCCAAGCTCAGCTTTCTTGGGCAGCAGTTTGAAGAAGGTCAACAACACAAGACTTCCCTACCAAAAATCTGTATCTGCAGGTAACTTGAGAATCACAGCAGAAATCGATGAGGGAAAGCAGTCGAAATCAGACAAGTGGAGAGGCCTTGCTTATGACATTTCTGATGACCAACAAGACATTACAAGAGGAAAGGGATTGGTTGATACTGTGTTCCAAGCTCCCAGTGGGACTGGGACTCACAATGCCATCATGAGCTCATACGATTACATCAGCACCGGACTTCGCACGTACAACTTGGATAACAACATGGATGGGTTCTACATAGCTCCAGCTTTCATGGACAAGCTTGTGGTTCATATCACCAAGAACTTCATGAGCCTGCCTAACATTAAGGTTCCCCTTATTCTTGGTATATGGGGAGGAAAAGGTCAGGGAAAATCATTCCAGTGTGAGCTTGTGTTTGCCAAGATGGGAATTACCCCCATCATGATGAGTGCTGGAGAATTAGAAAGTGGGAATGCAGGAGAGCCAGCAAAGTTGATCAGACAAAGGTATCGAGAGGCTTCAGACATCATCCGAAAAGGAAAAATGTGTGCCCTCTTCATCAATGATCTCGACGCAGGTGCTGGTAGGATGGGTGGAACCACACAGTATACTGTGAACAACCAGATGGTCAACGCAACCCTCATGAACATTGCTGATAACCCAACAAATGTGCAGCTTCCCGGTATGTACAACAAGGAGGACAATGCTAGAGTGCCGGTTATAGTCACTGGTAATGACTTCTCTACACTCTATGCGCCTCTTATCCGTGATGGTCGTATGGAGAAGTTTTACTGGGCTCCCACAAGGGAAGACCGGATTGGTGTTTGCATCGGAATCTTTAAGACTGACAATATTCCTGAGGAGGCTGTTGTTAAGATTGTAGACACATTCCCTGGTCAATCTATTGATTTCTTTGGTGCCCTTCGAGCTCGAGTCTACGACGATGAAGTGAGGAAGTGGATATCCGGTGTTGGAGTAGACGGCATTGGGAAAAAGCTTGTGAACTCAAAAGAAGGTCTCCCAACTTTTGAGCAGCCTAAGATGACACTGGAGAAGCTTCTTGAGTATGGTAACATGCTTGTTCAAGAGCAGGACAATGTCAAGAGAGTTCAACTTGCTGACACTTACTTGAGTCAAGCAGCACTTGGAGATGCTAACCAGGATTCTATTAAGAGAGGAGATTTCTATGGCCAGGCAGCCCAACAAGTTCATGTACCAGTTCCTGAAGGTTGTACTGATCGAACAGCCGCAAACTATGACCCAACAGCTAGAAGTGACGATGGTAGCTGTGAGTACCCACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

477

Amino Acids

51.96

Weight (kDa)

5.84

Isoelectric Point (pI)

35.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 165 - 308 7.4e-17 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 311 - 406 5.5e-40 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 692
Acc36I ACCTGC 2 cut(s) 149, 692
AccB1I GGYRCC 1 cut(s) 1043
AccI GTMKAC 4 cut(s) 734, 1008, 1062, 1104
AciI CCGC 1 cut(s) 1374
AcoI YGGCCR 1 cut(s) 1315
AcuI CTGAAG 2 cut(s) 630, 1371
AfaI GTAC 5 cut(s) 371, 815, 1341, 1360, 1421
AgsI TTSAA 4 cut(s) 55, 112, 1211, 1238
AjnI CCWGG 3 cut(s) 1018, 1284, 1317
AjuI GAANNNNNNNTTGG 2 cut(s) 927, 959
Alw21I GWGCWC 2 cut(s) 338, 1057
Alw26I GTCTC 2 cut(s) 19, 1148
AlwNI CAGNNNCTG 2 cut(s) 707, 1349
Ama87I CYCGRG 1 cut(s) 1056
AoxI GGCC 2 cut(s) 223, 1315
ApeKI GCWGC 5 cut(s) 102, 799, 1159, 1265, 1322
AspLEI GCGC 1 cut(s) 883
AsuC2I CCSGG 1 cut(s) 807
AsuHPI GGTGA 1 cut(s) 433
AvaI CYCGRG 1 cut(s) 1056
AxyI CCTNAGG 1 cut(s) 984
BaeGI GKGCMC 2 cut(s) 679, 1048
BaeI ACNNNNGTAYC 2 cut(s) 617, 650
BalI TGGCCA 1 cut(s) 1317
BanI GGYRCC 1 cut(s) 1043
BanII GRGCYC 3 cut(s) 338, 925, 1057
BbsI GAAGAC 1 cut(s) 942
Bbv12I GWGCWC 2 cut(s) 338, 1057
BbvI GCAGC 5 cut(s) 114, 811, 1171, 1277, 1334
BccI CCATC 8 cut(s) 335, 386, 544, 572, 709, 745, 890, 1400
BceAI ACGGC 1 cut(s) 1123
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BciT130I CCWGG 3 cut(s) 1020, 1286, 1319
BclI TGATCA 1 cut(s) 621
BcnI CCSGG 1 cut(s) 807
BcoDI GTCTC 2 cut(s) 19, 1148
BfaI CTAG 2 cut(s) 834, 1395
BfmI CTRYAG 1 cut(s) 156
BfuAI ACCTGC 2 cut(s) 149, 692
BisI GCNGC 6 cut(s) 103, 800, 1160, 1266, 1323, 1374
BlpI GCTNAGC 1 cut(s) 88
BlsI GCNGC 6 cut(s) 104, 801, 1161, 1267, 1324, 1375
Bme1390I CCNGG 4 cut(s) 807, 1020, 1286, 1319
BmeT110I CYCGRG 1 cut(s) 1056
BmiI GGNNCC 4 cut(s) 477, 724, 924, 1045
BmrFI CCNGG 4 cut(s) 807, 1020, 1286, 1319
BmrI ACTGGG 3 cut(s) 297, 321, 928
BmsI GCATC 2 cut(s) 963, 1267
BmuI ACTGGG 3 cut(s) 297, 321, 928
BpiI GAAGAC 1 cut(s) 942
BpmI CTGGAG 3 cut(s) 393, 600, 1196
Bpu1102I GCTNAGC 1 cut(s) 88
BpuEI CTTGAG 4 cut(s) 67, 187, 1208, 1276
BpuMI CCSGG 1 cut(s) 807
Bsa29I ATCGAT 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 369
BsaI GGTCTC 1 cut(s) 1148
BsaJI CCNNGG 1 cut(s) 1018
BsaWI WCCGGW 3 cut(s) 356, 939, 1091
Bse118I RCCGGY 1 cut(s) 841
Bse1I ACTGG 7 cut(s) 303, 316, 526, 859, 923, 1179, 1343
Bse21I CCTNAGG 1 cut(s) 984
Bse3DI GCAATG 1 cut(s) 774
BseBI CCWGG 3 cut(s) 1020, 1286, 1319
BseCI ATCGAT 1 cut(s) 186
BseDI CCNNGG 1 cut(s) 1018
BseGI GGATG 3 cut(s) 397, 654, 720
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 2 cut(s) 102, 975
BseNI ACTGG 7 cut(s) 303, 316, 526, 859, 923, 1179, 1343
BseRI GAGGAG 2 cut(s) 1001, 1317
BseSI GKGCMC 2 cut(s) 679, 1048
BseXI GCAGC 5 cut(s) 114, 811, 1171, 1277, 1334
BsgI GTGCAG 1 cut(s) 818
BshFI GGCC 2 cut(s) 225, 1317
BshNI GGYRCC 1 cut(s) 1043
BshVI ATCGAT 1 cut(s) 186
BsiHKAI GWGCWC 2 cut(s) 338, 1057
BsiHKCI CYCGRG 1 cut(s) 1056
BsiSI CCGG 5 cut(s) 357, 807, 842, 940, 1092
BslFI GGGAC 2 cut(s) 322, 328
BsmAI GTCTC 2 cut(s) 19, 1148
BsmFI GGGAC 2 cut(s) 322, 328
BsmI GAATGC 1 cut(s) 604
BsnI GGCC 2 cut(s) 225, 1317
Bso31I GGTCTC 1 cut(s) 1148
BsoBI CYCGRG 1 cut(s) 1056
Bsp1286I GDGCHC 5 cut(s) 338, 679, 925, 1048, 1057
Bsp1407I TGTACA 1 cut(s) 813
Bsp143I GATC 3 cut(s) 621, 691, 1363
Bsp1720I GCTNAGC 1 cut(s) 88
BspACI CCGC 1 cut(s) 1374
BspANI GGCC 2 cut(s) 225, 1317
BspCNI CTCAG 2 cut(s) 101, 976
BspDI ATCGAT 1 cut(s) 186
BspHI TCATGA 4 cut(s) 330, 453, 567, 768
BspLI GGNNCC 4 cut(s) 477, 724, 924, 1045
BspMAI CTGCAG 1 cut(s) 160
BspMI ACCTGC 2 cut(s) 149, 692
BspT107I GGYRCC 1 cut(s) 1043
BspTNI GGTCTC 1 cut(s) 1148
BsrDI GCAATG 1 cut(s) 774
BsrFI RCCGGY 1 cut(s) 841
BsrGI TGTACA 1 cut(s) 813
BsrI ACTGG 7 cut(s) 303, 316, 526, 859, 923, 1179, 1343
BssAI RCCGGY 1 cut(s) 841
BssECI CCNNGG 1 cut(s) 1018
BssMI GATC 3 cut(s) 621, 691, 1363
BssNAI GTATAC 1 cut(s) 735
Bst1107I GTATAC 1 cut(s) 735
Bst2UI CCWGG 3 cut(s) 1020, 1286, 1319
Bst4CI ACNGT 5 cut(s) 13, 22, 289, 732, 739
Bst6I CTCTTC 1 cut(s) 686
BstAUI TGTACA 1 cut(s) 813
BstBAI YACGTR 1 cut(s) 369
BstC8I GCNNGC 2 cut(s) 461, 612
BstDEI CTNAG 3 cut(s) 88, 984, 1164
BstF5I GGATG 3 cut(s) 397, 654, 720
BstHHI GCGC 1 cut(s) 883
BstKTI GATC 3 cut(s) 624, 694, 1366
BstMAI GTCTC 2 cut(s) 19, 1148
BstMBI GATC 3 cut(s) 621, 691, 1363
BstMWI GCNNNNNNNGC 2 cut(s) 57, 1052
BstNI CCWGG 3 cut(s) 1020, 1286, 1319
BstNSI RCATGY 1 cut(s) 1204
BstSCI CCNGG 4 cut(s) 805, 1018, 1284, 1317
BstSFI CTRYAG 1 cut(s) 156
BstSLI GKGCMC 2 cut(s) 679, 1048
BstV1I GCAGC 5 cut(s) 114, 811, 1171, 1277, 1334
BstV2I GAAGAC 1 cut(s) 942
BstZ17I GTATAC 1 cut(s) 735
Bsu15I ATCGAT 1 cut(s) 186
Bsu36I CCTNAGG 1 cut(s) 984
BsuRI GGCC 2 cut(s) 225, 1317
BsuTUI ATCGAT 1 cut(s) 186
BtsCI GGATG 3 cut(s) 397, 654, 720
BtsIMutI CAGTG 4 cut(s) 310, 533, 852, 1172
BveI ACCTGC 2 cut(s) 149, 692
Cac8I GCNNGC 2 cut(s) 461, 612
CaiI CAGNNNCTG 2 cut(s) 707, 1349
CciI TCATGA 4 cut(s) 330, 453, 567, 768
CfoI GCGC 1 cut(s) 883
Cfr10I RCCGGY 1 cut(s) 841
ClaI ATCGAT 1 cut(s) 186
CseI GACGC 1 cut(s) 707
Csp6I GTAC 5 cut(s) 370, 814, 1340, 1359, 1420
CviAII CATG 8 cut(s) 331, 388, 418, 454, 568, 769, 1201, 1337
CviQI GTAC 5 cut(s) 370, 814, 1340, 1359, 1420
DdeI CTNAG 3 cut(s) 88, 984, 1164
DpnI GATC 3 cut(s) 623, 693, 1365
DpnII GATC 3 cut(s) 621, 691, 1363
EaeI YGGCCR 1 cut(s) 1315
Eam1104I CTCTTC 1 cut(s) 686
EarI CTCTTC 1 cut(s) 686
Ecl136II GAGCTC 2 cut(s) 336, 1055
Eco147I AGGCCT 1 cut(s) 225
Eco24I GRGCYC 3 cut(s) 338, 925, 1057
Eco31I GGTCTC 1 cut(s) 1148
Eco32I GATATC 1 cut(s) 1089
Eco53kI GAGCTC 2 cut(s) 336, 1055
Eco57I CTGAAG 2 cut(s) 630, 1371
Eco81I CCTNAGG 1 cut(s) 984
Eco88I CYCGRG 1 cut(s) 1056
EcoICRI GAGCTC 2 cut(s) 336, 1055
EcoRII CCWGG 3 cut(s) 1018, 1284, 1317
EcoRV GATATC 1 cut(s) 1089
EcoT38I GRGCYC 3 cut(s) 338, 925, 1057
FaeI CATG 8 cut(s) 334, 391, 421, 457, 571, 772, 1204, 1340
FalI AAGNNNNNCTT 4 cut(s) 76, 108, 1254, 1286
FaqI GGGAC 2 cut(s) 322, 328
FatI CATG 8 cut(s) 330, 387, 417, 453, 567, 768, 1200, 1336
FbaI TGATCA 1 cut(s) 621
FblI GTMKAC 4 cut(s) 734, 1008, 1062, 1104
Fnu4HI GCNGC 6 cut(s) 103, 800, 1160, 1266, 1323, 1374
FokI GGATG 3 cut(s) 404, 641, 727
FriOI GRGCYC 3 cut(s) 338, 925, 1057
Fsp4HI GCNGC 6 cut(s) 103, 800, 1160, 1266, 1323, 1374
FspBI CTAG 2 cut(s) 834, 1395
GlaI GCGC 1 cut(s) 882
GluI GCNGC 6 cut(s) 103, 800, 1160, 1266, 1323, 1374
GsuI CTGGAG 3 cut(s) 393, 600, 1196
HaeIII GGCC 2 cut(s) 225, 1317
HapII CCGG 5 cut(s) 357, 807, 842, 940, 1092
HgaI GACGC 1 cut(s) 707
HhaI GCGC 1 cut(s) 883
Hin1II CATG 8 cut(s) 334, 391, 421, 457, 571, 772, 1204, 1340
Hin6I GCGC 1 cut(s) 881
HinP1I GCGC 1 cut(s) 881
HincII GTYRAC 3 cut(s) 34, 121, 757
HindII GTYRAC 3 cut(s) 34, 121, 757
HindIII AAGCTT 3 cut(s) 425, 1121, 1181
HinfI GANTC 6 cut(s) 171, 316, 960, 1059, 1258, 1289
HpaI GTTAAC 1 cut(s) 34
HpaII CCGG 5 cut(s) 357, 807, 842, 940, 1092
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 9 cut(s) 34, 121, 735, 742, 757, 1009, 1063, 1105, 1129
Hpy188I TCNGA 6 cut(s) 211, 244, 626, 649, 659, 959
Hpy8I GTNNAC 9 cut(s) 34, 121, 735, 742, 757, 1009, 1063, 1105, 1129
Hpy99I CGWCG 2 cut(s) 701, 1070
HpyAV CCTTC 4 cut(s) 109, 1058, 1133, 1346
HpyCH4III ACNGT 5 cut(s) 13, 22, 289, 732, 739
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 4 cut(s) 158, 602, 799, 954
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 1052
HpyF3I CTNAG 3 cut(s) 88, 984, 1164
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 8 cut(s) 334, 391, 421, 457, 571, 772, 1204, 1340
HspAI GCGC 1 cut(s) 881
Ksp22I TGATCA 1 cut(s) 621
KspAI GTTAAC 1 cut(s) 34
Kzo9I GATC 3 cut(s) 621, 691, 1363
LmnI GCTCC 4 cut(s) 26, 304, 412, 928
Lsp1109I GCAGC 5 cut(s) 114, 811, 1171, 1277, 1334
LweI GCATC 2 cut(s) 963, 1267
MaeI CTAG 2 cut(s) 834, 1395
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 4 cut(s) 161, 850, 1196, 1400
MalI GATC 3 cut(s) 623, 693, 1365
MboI GATC 3 cut(s) 621, 691, 1363
MboII GAAGA 3 cut(s) 124, 673, 947
MhlI GDGCHC 5 cut(s) 338, 679, 925, 1048, 1057
MlsI TGGCCA 1 cut(s) 1317
MluCI AATT 2 cut(s) 555, 584
MluNI TGGCCA 1 cut(s) 1317
MlyI GAGTC 3 cut(s) 310, 1068, 1267
MmeI TCCRAC 2 cut(s) 4, 1078
Mox20I TGGCCA 1 cut(s) 1317
MscI TGGCCA 1 cut(s) 1317
MseI TTAA 5 cut(s) 33, 471, 966, 999, 1296
MslI CAYNNNNRTG 2 cut(s) 526, 569
Msp20I TGGCCA 1 cut(s) 1317
MspI CCGG 5 cut(s) 357, 807, 842, 940, 1092
MspR9I CCNGG 4 cut(s) 807, 1020, 1286, 1319
Mva1269I GAATGC 1 cut(s) 604
MvaI CCWGG 3 cut(s) 1020, 1286, 1319
MwoI GCNNNNNNNGC 2 cut(s) 57, 1052
NciI CCSGG 1 cut(s) 807
NdeII GATC 3 cut(s) 621, 691, 1363
NlaIII CATG 8 cut(s) 334, 391, 421, 457, 571, 772, 1204, 1340
NlaIV GGNNCC 4 cut(s) 477, 724, 924, 1045
NmuCI GTSAC 2 cut(s) 850, 1400
NspI RCATGY 1 cut(s) 1204
PaeR7I CTCGAG 1 cut(s) 1056
PagI TCATGA 4 cut(s) 330, 453, 567, 768
PaqCI CACCTGC 1 cut(s) 692
PceI AGGCCT 1 cut(s) 225
PctI GAATGC 1 cut(s) 604
PfeI GAWTC 3 cut(s) 171, 960, 1289
PflFI GACNNNGTC 1 cut(s) 1223
PkrI GCNGC 6 cut(s) 104, 801, 1161, 1267, 1324, 1375
PleI GAGTC 3 cut(s) 310, 1067, 1266
PpsI GAGTC 3 cut(s) 310, 1067, 1266
Ppu21I YACGTR 1 cut(s) 369
Psp124BI GAGCTC 2 cut(s) 338, 1057
Psp6I CCWGG 3 cut(s) 1018, 1284, 1317
PspGI CCWGG 3 cut(s) 1018, 1284, 1317
PspN4I GGNNCC 4 cut(s) 477, 724, 924, 1045
PspXI VCTCGAGB 1 cut(s) 1056
PstI CTGCAG 1 cut(s) 160
PstNI CAGNNNCTG 2 cut(s) 707, 1349
PsyI GACNNNGTC 1 cut(s) 1223
RsaI GTAC 5 cut(s) 371, 815, 1341, 1360, 1421
RsaNI GTAC 5 cut(s) 370, 814, 1340, 1359, 1420
RseI CAYNNNNRTG 2 cut(s) 526, 569
SacI GAGCTC 2 cut(s) 338, 1057
SaqAI TTAA 5 cut(s) 33, 471, 966, 999, 1296
SatI GCNGC 6 cut(s) 103, 800, 1160, 1266, 1323, 1374
Sau3AI GATC 3 cut(s) 621, 691, 1363
SchI GAGTC 3 cut(s) 310, 1068, 1267
ScrFI CCNGG 4 cut(s) 807, 1020, 1286, 1319
SduI GDGCHC 5 cut(s) 338, 679, 925, 1048, 1057
SfaNI GCATC 2 cut(s) 963, 1267
SfcI CTRYAG 1 cut(s) 156
Sfr274I CTCGAG 1 cut(s) 1056
SlaI CTCGAG 1 cut(s) 1056
SmiMI CAYNNNNRTG 2 cut(s) 526, 569
SmlI CTYRAG 5 cut(s) 46, 166, 1056, 1187, 1255
SmoI CTYRAG 5 cut(s) 46, 166, 1056, 1187, 1255
Sse9I AATT 2 cut(s) 555, 584
SseBI AGGCCT 1 cut(s) 225
SsiI CCGC 1 cut(s) 1374
SspI AATATT 1 cut(s) 979
SspMI CTAG 2 cut(s) 834, 1395
SstI GAGCTC 2 cut(s) 338, 1057
StuI AGGCCT 1 cut(s) 225
StyD4I CCNGG 4 cut(s) 805, 1018, 1284, 1317
TaaI ACNGT 5 cut(s) 13, 22, 289, 732, 739
TaiI ACGT 1 cut(s) 371
TaqI TCGA 7 cut(s) 186, 203, 637, 696, 1051, 1057, 1366
TasI AATT 2 cut(s) 555, 584
TatI WGTACW 2 cut(s) 813, 1358
TauI GCSGC 1 cut(s) 1376
TfiI GAWTC 3 cut(s) 171, 960, 1289
Tru1I TTAA 5 cut(s) 33, 471, 966, 999, 1296
Tru9I TTAA 5 cut(s) 33, 471, 966, 999, 1296
TscAI CASTG 4 cut(s) 310, 533, 859, 1179
TseFI GTSAC 2 cut(s) 850, 1400
TseI GCWGC 5 cut(s) 102, 799, 1159, 1265, 1322
Tsp45I GTSAC 2 cut(s) 850, 1400
TspDTI ATGAA 7 cut(s) 406, 425, 442, 673, 785, 1086, 1325
TspGWI ACGGA 1 cut(s) 881
TspRI CASTG 4 cut(s) 310, 533, 859, 1179
Tth111I GACNNNGTC 1 cut(s) 1223
XceI RCATGY 1 cut(s) 1204
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XhoI CTCGAG 1 cut(s) 1056
XmiI GTMKAC 4 cut(s) 734, 1008, 1062, 1104
XspI CTAG 2 cut(s) 834, 1395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.