Rh5CG474300

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000911
Physical Location & Seq
Reverse (-)
66262609 .. 66266115
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG474300.1

Sequence Viewer

Length: 1281 bp
ATGCAGTTGAGCTTGAACAGCTCTGGTGCTGGAAGCTCAATCCCAAGCTCAGCTTTCTTGGGCAGCAGTTTGAAGAAGGTCAACAACACAAGACTTCCCTACCAAAAATCTGTATCTGCAGGTAGCTTGAGAATCACAGCAGAAATCGATGAGGGAAAGCAGTCGAAATCAGACAAGTGGAAAGGCCTTGCTTATGACATTTCTGATGACCAACAAGACATTACAAGAGGAAAGGGAATGGTTGATTCTGTCTTCCAAGCTCCCACTGGGACTGGGACTCACAATGCCATCATGAGCTCATATGATTACATCAGCACCGGACTTCGCACGTACAACTTGGATAACAACATGGATGGGTTTTACATAGCTCCAGCTTTCATGGACAAGCTTGTGGTTCATATCACCAAGAACTTCATGAGCCTGCCTAACATTAAGGTTCCCCTTATTCTTGGTATATGGGGAGGAAAAGGTCAGGGAAAATCATTCCAGTGTGAGCTTGTGTTTGCCAAGATGGGAATTACCCCCATCATGATGAGTGCTGGAGAATTAGAAAGTGGGAATGCAGGAGAGCCAGCAAAGTTGATCAGACAAAGGTATCGGGAGGCTTCAGACATCATCCGAAAAGGAAAAATGTGTGCCCTCTTCATCAATGATCTCGATGCAGGTGCTGGTAGGATGGGTGGAACCACACAGTACACTGTGAACAACCAGATGGTCAACGCAACCCTGATGAACATTGCTGATAACCCAACAAATGTGCAGCTTCCCGGTATGTACAACAAGGAGGACAATGCTAGAGTGCCGGTTATAGTCACCGGTAATGACTTCTCTACACTCTATGCGCCTCTTATCCGTGATGGTCGTATGGAGAAGTTTTACTGGGCTCCCACAAGGGAAGACCGGATTGGTGTTTGCATCGGAATCTTCAAGACTGACAATATTCCTGAGGAGGCTGTTGTTAAGATTGTAGACACATTCCCTGGTCAATCTATTGATTTCTTTGGTGCCCTTCGAGCTCGAGTCTATGATGATGAAGTGAGGAAGTGGATATCCGGTGTTGGAGTAGACGGCATTGGGAAAAAGCTTGTGAACTCAAAAGAAGGTCTCCCAACTTTTGAGCAGCCTAAGATGACACTGGAGAAGCTTCTTGAGTATGGTAACATGCTTGTTCAAGAGCAGGACAATGTCAAGAGAGTTCAACTTGCTGACACTTACTTGAGTCAAGCAGCACTTGGAGATGCTAACCAGGATTCTATTAAAAGAGGAGATTTCTATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

426

Amino Acids

46.77

Weight (kDa)

6.78

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 152 - 295 5.9e-17 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 298 - 393 4.6e-40 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 653
Acc36I ACCTGC 2 cut(s) 110, 653
AccB1I GGYRCC 1 cut(s) 1004
AccI GTMKAC 2 cut(s) 969, 1065
AcuI CTGAAG 1 cut(s) 591
AfaI GTAC 3 cut(s) 332, 695, 776
AgeI ACCGGT 1 cut(s) 815
AgsI TTSAA 5 cut(s) 16, 73, 928, 1172, 1199
AjnI CCWGG 2 cut(s) 979, 1245
AjuI GAANNNNNNNTTGG 2 cut(s) 888, 920
Alw21I GWGCWC 2 cut(s) 299, 1018
Alw26I GTCTC 1 cut(s) 1109
AlwNI CAGNNNCTG 1 cut(s) 668
Ama87I CYCGRG 1 cut(s) 1017
AoxI GGCC 1 cut(s) 184
ApeKI GCWGC 4 cut(s) 63, 760, 1120, 1226
AsiGI ACCGGT 1 cut(s) 815
AspLEI GCGC 1 cut(s) 844
AsuC2I CCSGG 1 cut(s) 768
AsuHPI GGTGA 2 cut(s) 394, 805
AvaI CYCGRG 1 cut(s) 1017
AxyI CCTNAGG 1 cut(s) 945
BaeGI GKGCMC 2 cut(s) 640, 1009
BaeI ACNNNNGTAYC 2 cut(s) 578, 611
BanI GGYRCC 1 cut(s) 1004
BanII GRGCYC 3 cut(s) 299, 886, 1018
BbsI GAAGAC 2 cut(s) 244, 903
Bbv12I GWGCWC 2 cut(s) 299, 1018
BbvI GCAGC 4 cut(s) 75, 772, 1132, 1238
BccI CCATC 7 cut(s) 296, 347, 505, 533, 670, 706, 851
BceAI ACGGC 1 cut(s) 1084
BcgI CGANNNNNNTGC 2 cut(s) 647, 681
BciT130I CCWGG 2 cut(s) 981, 1247
BclI TGATCA 1 cut(s) 582
BcnI CCSGG 1 cut(s) 768
BcoDI GTCTC 1 cut(s) 1109
BfaI CTAG 1 cut(s) 795
BfmI CTRYAG 1 cut(s) 117
BfuAI ACCTGC 2 cut(s) 110, 653
BisI GCNGC 4 cut(s) 64, 761, 1121, 1227
BlpI GCTNAGC 1 cut(s) 49
BlsI GCNGC 4 cut(s) 65, 762, 1122, 1228
Bme1390I CCNGG 3 cut(s) 768, 981, 1247
BmeT110I CYCGRG 1 cut(s) 1017
BmiI GGNNCC 4 cut(s) 438, 685, 885, 1006
BmrFI CCNGG 3 cut(s) 768, 981, 1247
BmrI ACTGGG 3 cut(s) 276, 282, 889
BmsI GCATC 3 cut(s) 649, 924, 1228
BmuI ACTGGG 3 cut(s) 276, 282, 889
BpiI GAAGAC 2 cut(s) 244, 903
BpmI CTGGAG 3 cut(s) 354, 561, 1157
Bpu1102I GCTNAGC 1 cut(s) 49
BpuEI CTTGAG 3 cut(s) 148, 1169, 1237
BpuMI CCSGG 1 cut(s) 768
Bsa29I ATCGAT 1 cut(s) 147
BsaAI YACGTR 1 cut(s) 330
BsaI GGTCTC 1 cut(s) 1109
BsaJI CCNNGG 1 cut(s) 979
BsaWI WCCGGW 4 cut(s) 317, 815, 900, 1052
Bse118I RCCGGY 2 cut(s) 802, 815
Bse1I ACTGG 5 cut(s) 271, 277, 487, 884, 1140
Bse21I CCTNAGG 1 cut(s) 945
Bse3DI GCAATG 1 cut(s) 735
BseBI CCWGG 2 cut(s) 981, 1247
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 979
BseGI GGATG 3 cut(s) 358, 615, 681
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 2 cut(s) 63, 936
BseNI ACTGG 5 cut(s) 271, 277, 487, 884, 1140
BseRI GAGGAG 2 cut(s) 962, 1278
BseSI GKGCMC 2 cut(s) 640, 1009
BseXI GCAGC 4 cut(s) 75, 772, 1132, 1238
BsgI GTGCAG 1 cut(s) 779
BshFI GGCC 1 cut(s) 186
BshNI GGYRCC 1 cut(s) 1004
BshTI ACCGGT 1 cut(s) 815
BshVI ATCGAT 1 cut(s) 147
BsiHKAI GWGCWC 2 cut(s) 299, 1018
BsiHKCI CYCGRG 1 cut(s) 1017
BsiSI CCGG 6 cut(s) 318, 768, 803, 816, 901, 1053
BslFI GGGAC 2 cut(s) 283, 289
BsmAI GTCTC 1 cut(s) 1109
BsmFI GGGAC 2 cut(s) 283, 289
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 1 cut(s) 186
Bso31I GGTCTC 1 cut(s) 1109
BsoBI CYCGRG 1 cut(s) 1017
Bsp1286I GDGCHC 5 cut(s) 299, 640, 886, 1009, 1018
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 2 cut(s) 582, 652
Bsp1720I GCTNAGC 1 cut(s) 49
BspANI GGCC 1 cut(s) 186
BspCNI CTCAG 2 cut(s) 62, 937
BspDI ATCGAT 1 cut(s) 147
BspHI TCATGA 3 cut(s) 291, 414, 528
BspLI GGNNCC 4 cut(s) 438, 685, 885, 1006
BspMAI CTGCAG 1 cut(s) 121
BspMI ACCTGC 2 cut(s) 110, 653
BspT107I GGYRCC 1 cut(s) 1004
BspTNI GGTCTC 1 cut(s) 1109
BsrDI GCAATG 1 cut(s) 735
BsrFI RCCGGY 2 cut(s) 802, 815
BsrGI TGTACA 1 cut(s) 774
BsrI ACTGG 5 cut(s) 271, 277, 487, 884, 1140
BssAI RCCGGY 2 cut(s) 802, 815
BssECI CCNNGG 1 cut(s) 979
BssMI GATC 2 cut(s) 582, 652
Bst2UI CCWGG 2 cut(s) 981, 1247
Bst4CI ACNGT 2 cut(s) 693, 700
Bst6I CTCTTC 1 cut(s) 647
BstAUI TGTACA 1 cut(s) 774
BstBAI YACGTR 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 422, 573
BstDEI CTNAG 3 cut(s) 49, 945, 1125
BstF5I GGATG 3 cut(s) 358, 615, 681
BstHHI GCGC 1 cut(s) 844
BstKTI GATC 2 cut(s) 585, 655
BstMAI GTCTC 1 cut(s) 1109
BstMBI GATC 2 cut(s) 582, 652
BstMWI GCNNNNNNNGC 2 cut(s) 18, 1013
BstNI CCWGG 2 cut(s) 981, 1247
BstNSI RCATGY 1 cut(s) 1165
BstSCI CCNGG 3 cut(s) 766, 979, 1245
BstSFI CTRYAG 1 cut(s) 117
BstSLI GKGCMC 2 cut(s) 640, 1009
BstV1I GCAGC 4 cut(s) 75, 772, 1132, 1238
BstV2I GAAGAC 2 cut(s) 244, 903
Bsu15I ATCGAT 1 cut(s) 147
Bsu36I CCTNAGG 1 cut(s) 945
BsuRI GGCC 1 cut(s) 186
BsuTUI ATCGAT 1 cut(s) 147
BtsCI GGATG 3 cut(s) 358, 615, 681
BtsIMutI CAGTG 4 cut(s) 264, 494, 696, 1133
BveI ACCTGC 2 cut(s) 110, 653
Cac8I GCNNGC 2 cut(s) 422, 573
CaiI CAGNNNCTG 1 cut(s) 668
CciI TCATGA 3 cut(s) 291, 414, 528
CfoI GCGC 1 cut(s) 844
Cfr10I RCCGGY 2 cut(s) 802, 815
ClaI ATCGAT 1 cut(s) 147
Csp6I GTAC 3 cut(s) 331, 694, 775
CspAI ACCGGT 1 cut(s) 815
CviAII CATG 6 cut(s) 292, 349, 379, 415, 529, 1162
CviQI GTAC 3 cut(s) 331, 694, 775
DdeI CTNAG 3 cut(s) 49, 945, 1125
DpnI GATC 2 cut(s) 584, 654
DpnII GATC 2 cut(s) 582, 652
Eam1104I CTCTTC 1 cut(s) 647
EarI CTCTTC 1 cut(s) 647
Ecl136II GAGCTC 2 cut(s) 297, 1016
Eco147I AGGCCT 1 cut(s) 186
Eco24I GRGCYC 3 cut(s) 299, 886, 1018
Eco31I GGTCTC 1 cut(s) 1109
Eco32I GATATC 1 cut(s) 1050
Eco53kI GAGCTC 2 cut(s) 297, 1016
Eco57I CTGAAG 1 cut(s) 591
Eco81I CCTNAGG 1 cut(s) 945
Eco88I CYCGRG 1 cut(s) 1017
EcoICRI GAGCTC 2 cut(s) 297, 1016
EcoRII CCWGG 2 cut(s) 979, 1245
EcoRV GATATC 1 cut(s) 1050
EcoT38I GRGCYC 3 cut(s) 299, 886, 1018
FaeI CATG 6 cut(s) 295, 352, 382, 418, 532, 1165
FalI AAGNNNNNCTT 4 cut(s) 37, 69, 1215, 1247
FaqI GGGAC 2 cut(s) 283, 289
FatI CATG 6 cut(s) 291, 348, 378, 414, 528, 1161
FauNDI CATATG 1 cut(s) 301
FbaI TGATCA 1 cut(s) 582
FblI GTMKAC 2 cut(s) 969, 1065
Fnu4HI GCNGC 4 cut(s) 64, 761, 1121, 1227
FokI GGATG 3 cut(s) 365, 602, 688
FriOI GRGCYC 3 cut(s) 299, 886, 1018
Fsp4HI GCNGC 4 cut(s) 64, 761, 1121, 1227
FspBI CTAG 1 cut(s) 795
GlaI GCGC 1 cut(s) 843
GluI GCNGC 4 cut(s) 64, 761, 1121, 1227
GsuI CTGGAG 3 cut(s) 354, 561, 1157
HaeIII GGCC 1 cut(s) 186
HapII CCGG 6 cut(s) 318, 768, 803, 816, 901, 1053
HhaI GCGC 1 cut(s) 844
Hin1II CATG 6 cut(s) 295, 352, 382, 418, 532, 1165
Hin6I GCGC 1 cut(s) 842
HinP1I GCGC 1 cut(s) 842
HincII GTYRAC 2 cut(s) 82, 718
HindII GTYRAC 2 cut(s) 82, 718
HindIII AAGCTT 3 cut(s) 386, 1082, 1142
HinfI GANTC 7 cut(s) 132, 245, 277, 921, 1020, 1219, 1250
HpaII CCGG 6 cut(s) 318, 768, 803, 816, 901, 1053
HphI GGTGA 2 cut(s) 394, 805
Hpy166II GTNNAC 7 cut(s) 82, 696, 703, 718, 970, 1066, 1090
Hpy188I TCNGA 6 cut(s) 172, 205, 587, 610, 620, 920
Hpy8I GTNNAC 7 cut(s) 82, 696, 703, 718, 970, 1066, 1090
HpyAV CCTTC 3 cut(s) 70, 1019, 1094
HpyCH4III ACNGT 2 cut(s) 693, 700
HpyCH4IV ACGT 1 cut(s) 329
HpyCH4V TGCA 6 cut(s) 4, 119, 563, 662, 760, 915
HpyF10VI GCNNNNNNNGC 2 cut(s) 18, 1013
HpyF3I CTNAG 3 cut(s) 49, 945, 1125
HpySE526I ACGT 1 cut(s) 329
Hsp92II CATG 6 cut(s) 295, 352, 382, 418, 532, 1165
HspAI GCGC 1 cut(s) 842
Ksp22I TGATCA 1 cut(s) 582
Kzo9I GATC 2 cut(s) 582, 652
LmnI GCTCC 3 cut(s) 265, 373, 889
Lsp1109I GCAGC 4 cut(s) 75, 772, 1132, 1238
LweI GCATC 3 cut(s) 649, 924, 1228
MaeI CTAG 1 cut(s) 795
MaeII ACGT 1 cut(s) 329
MaeIII GTNAC 2 cut(s) 811, 1157
MalI GATC 2 cut(s) 584, 654
MboI GATC 2 cut(s) 582, 652
MboII GAAGA 5 cut(s) 85, 244, 634, 908, 916
MhlI GDGCHC 5 cut(s) 299, 640, 886, 1009, 1018
MluCI AATT 2 cut(s) 516, 545
MlyI GAGTC 3 cut(s) 271, 1029, 1228
MmeI TCCRAC 1 cut(s) 1039
MseI TTAA 3 cut(s) 432, 960, 1257
MslI CAYNNNNRTG 2 cut(s) 487, 530
MspI CCGG 6 cut(s) 318, 768, 803, 816, 901, 1053
MspR9I CCNGG 3 cut(s) 768, 981, 1247
Mva1269I GAATGC 1 cut(s) 565
MvaI CCWGG 2 cut(s) 981, 1247
MwoI GCNNNNNNNGC 2 cut(s) 18, 1013
NciI CCSGG 1 cut(s) 768
NdeI CATATG 1 cut(s) 301
NdeII GATC 2 cut(s) 582, 652
NlaIII CATG 6 cut(s) 295, 352, 382, 418, 532, 1165
NlaIV GGNNCC 4 cut(s) 438, 685, 885, 1006
NmuCI GTSAC 1 cut(s) 811
NspI RCATGY 1 cut(s) 1165
PaeR7I CTCGAG 1 cut(s) 1017
PagI TCATGA 3 cut(s) 291, 414, 528
PaqCI CACCTGC 1 cut(s) 653
PceI AGGCCT 1 cut(s) 186
PctI GAATGC 1 cut(s) 565
PfeI GAWTC 4 cut(s) 132, 245, 921, 1250
PflFI GACNNNGTC 1 cut(s) 1184
PinAI ACCGGT 1 cut(s) 815
PkrI GCNGC 4 cut(s) 65, 762, 1122, 1228
PleI GAGTC 3 cut(s) 271, 1028, 1227
PpsI GAGTC 3 cut(s) 271, 1028, 1227
Ppu21I YACGTR 1 cut(s) 330
Psp124BI GAGCTC 2 cut(s) 299, 1018
Psp6I CCWGG 2 cut(s) 979, 1245
PspGI CCWGG 2 cut(s) 979, 1245
PspN4I GGNNCC 4 cut(s) 438, 685, 885, 1006
PspXI VCTCGAGB 1 cut(s) 1017
PstI CTGCAG 1 cut(s) 121
PstNI CAGNNNCTG 1 cut(s) 668
PsyI GACNNNGTC 1 cut(s) 1184
RsaI GTAC 3 cut(s) 332, 695, 776
RsaNI GTAC 3 cut(s) 331, 694, 775
RseI CAYNNNNRTG 2 cut(s) 487, 530
SacI GAGCTC 2 cut(s) 299, 1018
SaqAI TTAA 3 cut(s) 432, 960, 1257
SatI GCNGC 4 cut(s) 64, 761, 1121, 1227
Sau3AI GATC 2 cut(s) 582, 652
SchI GAGTC 3 cut(s) 271, 1029, 1228
ScrFI CCNGG 3 cut(s) 768, 981, 1247
SduI GDGCHC 5 cut(s) 299, 640, 886, 1009, 1018
SfaNI GCATC 3 cut(s) 649, 924, 1228
SfcI CTRYAG 1 cut(s) 117
Sfr274I CTCGAG 1 cut(s) 1017
SlaI CTCGAG 1 cut(s) 1017
SmiMI CAYNNNNRTG 2 cut(s) 487, 530
SmlI CTYRAG 4 cut(s) 127, 1017, 1148, 1216
SmoI CTYRAG 4 cut(s) 127, 1017, 1148, 1216
Sse9I AATT 2 cut(s) 516, 545
SseBI AGGCCT 1 cut(s) 186
SspI AATATT 1 cut(s) 940
SspMI CTAG 1 cut(s) 795
SstI GAGCTC 2 cut(s) 299, 1018
StuI AGGCCT 1 cut(s) 186
StyD4I CCNGG 3 cut(s) 766, 979, 1245
TaaI ACNGT 2 cut(s) 693, 700
TaiI ACGT 1 cut(s) 332
TaqI TCGA 5 cut(s) 147, 164, 657, 1012, 1018
TasI AATT 2 cut(s) 516, 545
TatI WGTACW 2 cut(s) 693, 774
TfiI GAWTC 4 cut(s) 132, 245, 921, 1250
Tru1I TTAA 3 cut(s) 432, 960, 1257
Tru9I TTAA 3 cut(s) 432, 960, 1257
TscAI CASTG 4 cut(s) 271, 494, 703, 1140
TseFI GTSAC 1 cut(s) 811
TseI GCWGC 4 cut(s) 63, 760, 1120, 1226
Tsp45I GTSAC 1 cut(s) 811
TspDTI ATGAA 6 cut(s) 367, 386, 403, 634, 746, 1047
TspGWI ACGGA 1 cut(s) 842
TspRI CASTG 4 cut(s) 271, 494, 703, 1140
Tth111I GACNNNGTC 1 cut(s) 1184
XceI RCATGY 1 cut(s) 1165
XcmI CCANNNNNNNNNTGG 1 cut(s) 263
XhoI CTCGAG 1 cut(s) 1017
XmiI GTMKAC 2 cut(s) 969, 1065
XspI CTAG 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.