Prupe.6G260200_v2.0.a1

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000911
Physical Location & Seq
Reverse (-)
25092579 .. 25095350
2772 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G260200.1

Sequence Viewer

Length: 1422 bp
ATGGCTGCTTCAATCTCAGGAATCATCGCGGCTGTTAACAGAGCACCGTTGAAGTTAAATGGCTCCGGTGCTGCAACTTCCTATCCAAGCTCTGCCTTCTTAGGCAACAGCCTGAAGAAGGCCACCTCCAAGATCACACACCTAAAGATTTCATCTCGGAATTTCAAGGTTTCTGCAGAATATGATGAGGAAAAGCAGACCTCAAAAGACAGGTGGGGAGGGCTTGCCTTTGACACTTCAGATGACCAGCAGGACATCACCAGAGGAAAGGGCATGGTGGACACTCTCTTCCAAGCCCCTATGGGTAGCGGGACCCACTATGCTGTCATGAGCTCCTATGATTACATCAGCACTGGTCTTCGCCAATTGGACAACATTATGGATGGCTACTACATTGCTCCAGCTTTCATGGACAAACTTGTTGTTCATATTACTAAGAACTTTTTGAACCTGCCAAACATTAAGGTTCCTCTTATTTTGGGTGTATGGGGAGGCAAAGGTCAAGGAAAGTCATTCCAATGTGAGCTTGTCTTTGCCAAAATGGGAATCAACCCTATTATGATGAGTGCTGGAGAATTGGAAAGTGGAAATGCTGGAGAGCCAGCAAAACTGATCAGGCAAAGGTACCGTGAGGCAGCAGACATCATCAGCAAGGGCAAAATGTGCTGCCTCTTCATCAATGATCTAGATGCAGGAGCTGGAAGACTTGGTGGAACTACTCAGTACACTGTCAACAATCAAATGGTGAATGCTACCCTCATGAACATTGCTGACAACCCAACCAATGTTCAACTCCCTGGCATGTACAACAAGCAGGAGAATCCCCGCGTTCCCATCATAGTCACTGGTAATGACTTCTCCACATTGTATGCCCCTCTCATCCGTGATGGACGTATGGAAAAGTTCTACTGGGCGCCTACTCGCGAAGACTGCATTGGCGTGTGCACAGGGATTTTCCGGGCTGACAATGTTCCTAGTGATGACATTGTCAAGCTTGTTGACACTTTCCCTGGTCAGTCCATTGACTTCTTTGGTGCTATAAGGGCCAGAGTTTATGATGATGAAGTGAGGAAATGGGTCGCCAGTGTGGGGGTAGAAAGTATTGGTAAGAGGCTTGTGAACTCAAAAGAGGGTCCTCCGACTTTCAATCAACCGAAGATGACTCTTGCGAAGCTGCTTGAGTATGGAAACTTGCTTGTGCAAGAGCAGGACAATGTGAAGAGAGTCCAATTGTCTGAAAAGTACTTGAAAGAGGCGGCTCTAGGAGACGCCAACGACGATGCCATCAAGAGTGGAAATTTCTATGGGAAAGCAGCCCAACTGGTTAACCTGCCTGTCCCTGAAGGTTGCACCGATCCATCTGCAGCAAACTATGATCCAACCGCGAGGAGTGACAACGGGAGCTGCCTGTACCAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

474

Amino Acids

51.64

Weight (kDa)

6.84

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 459, 1338
Acc65I GGTACC 1 cut(s) 624
AccB1I GGYRCC 2 cut(s) 624, 913
AccII CGCG 4 cut(s) 29, 828, 924, 1385
AciI CCGC 5 cut(s) 29, 309, 826, 1256, 1383
AclWI GGATC 2 cut(s) 1349, 1370
AcsI RAATTY 2 cut(s) 160, 1297
AcuI CTGAAG 3 cut(s) 134, 222, 1362
AcyI GRCGYC 2 cut(s) 914, 1269
AfaI GTAC 5 cut(s) 626, 725, 806, 1244, 1412
AfiI CCNNNNNNNGG 2 cut(s) 118, 1089
AgsI TTSAA 7 cut(s) 12, 52, 166, 448, 791, 1147, 1249
AjnI CCWGG 2 cut(s) 796, 1009
AjuI GAANNNNNNNTTGG 2 cut(s) 918, 950
AluBI AGCT 8 cut(s) 90, 333, 404, 526, 698, 994, 1174, 1404
AluI AGCT 8 cut(s) 90, 333, 404, 526, 698, 994, 1174, 1404
Alw21I GWGCWC 3 cut(s) 46, 335, 947
Alw26I GTCTC 1 cut(s) 1260
Alw44I GTGCAC 1 cut(s) 943
AlwI GGATC 2 cut(s) 1349, 1370
AlwNI CAGNNNCTG 1 cut(s) 698
AoxI GGCC 2 cut(s) 120, 1044
ApaLI GTGCAC 1 cut(s) 943
ApeKI GCWGC 8 cut(s) 5, 71, 635, 666, 1174, 1313, 1364, 1404
ApoI RAATTY 2 cut(s) 160, 1297
Asp718I GGTACC 1 cut(s) 624
AspLEI GCGC 1 cut(s) 916
AspS9I GGNCC 3 cut(s) 312, 1044, 1133
AsuC2I CCSGG 1 cut(s) 959
AsuHPI GGTGA 2 cut(s) 250, 757
AvaII GGWCC 2 cut(s) 312, 1133
BaeGI GKGCMC 1 cut(s) 947
BanI GGYRCC 2 cut(s) 624, 913
BanII GRGCYC 1 cut(s) 335
BbsI GAAGAC 3 cut(s) 350, 709, 933
Bbv12I GWGCWC 3 cut(s) 46, 335, 947
BbvI GCAGC 7 cut(s) 58, 647, 653, 1161, 1325, 1376, 1391
BccI CCATC 5 cut(s) 377, 842, 881, 1292, 1366
BcgI CGANNNNNNTGC 2 cut(s) 1343, 1377
BciT130I CCWGG 2 cut(s) 798, 1011
BclI TGATCA 1 cut(s) 612
BcnI CCSGG 1 cut(s) 959
BcoDI GTCTC 1 cut(s) 1260
BfaI CTAG 3 cut(s) 686, 975, 1262
BfmI CTRYAG 2 cut(s) 174, 1362
BfoI RGCGCY 1 cut(s) 917
BfuAI ACCTGC 2 cut(s) 459, 1338
BmcAI AGTACT 1 cut(s) 1244
Bme1390I CCNGG 3 cut(s) 798, 959, 1011
Bme18I GGWCC 2 cut(s) 312, 1133
BmgT120I GGNCC 3 cut(s) 312, 1044, 1133
BmiI GGNNCC 7 cut(s) 64, 313, 314, 468, 626, 915, 1134
BmrFI CCNGG 3 cut(s) 798, 959, 1011
BmrI ACTGGG 1 cut(s) 919
BmsI GCATC 2 cut(s) 679, 1270
BmuI ACTGGG 1 cut(s) 919
BpiI GAAGAC 3 cut(s) 350, 709, 933
BpmI CTGGAG 3 cut(s) 384, 591, 615
BpuEI CTTGAG 1 cut(s) 1199
BpuMI CCSGG 1 cut(s) 959
BsaHI GRCGYC 2 cut(s) 914, 1269
BsaJI CCNNGG 2 cut(s) 796, 1009
BsaWI WCCGGW 1 cut(s) 65
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 1089
Bse1I ACTGG 5 cut(s) 358, 850, 914, 1083, 1326
Bse3DI GCAATG 2 cut(s) 393, 765
BseBI CCWGG 2 cut(s) 798, 1011
BseDI CCNNGG 2 cut(s) 796, 1009
BseGI GGATG 2 cut(s) 388, 879
BseLI CCNNNNNNNGG 2 cut(s) 118, 1089
BseMI GCAATG 2 cut(s) 393, 765
BseMII CTCAG 2 cut(s) 30, 734
BseNI ACTGG 5 cut(s) 358, 850, 914, 1083, 1326
BseRI GAGGAG 1 cut(s) 1402
BseSI GKGCMC 1 cut(s) 947
BseXI GCAGC 7 cut(s) 58, 647, 653, 1161, 1325, 1376, 1391
Bsh1236I CGCG 4 cut(s) 29, 828, 924, 1385
BshFI GGCC 2 cut(s) 122, 1046
BshNI GGYRCC 2 cut(s) 624, 913
BsiHKAI GWGCWC 3 cut(s) 46, 335, 947
BsiSI CCGG 2 cut(s) 66, 958
BslFI GGGAC 2 cut(s) 325, 1322
BslI CCNNNNNNNGG 2 cut(s) 118, 1089
BsmAI GTCTC 1 cut(s) 1260
BsmBI CGTCTC 1 cut(s) 1260
BsmFI GGGAC 2 cut(s) 325, 1322
BsmI GAATGC 1 cut(s) 754
BsnI GGCC 2 cut(s) 122, 1046
Bsp1286I GDGCHC 3 cut(s) 46, 335, 947
Bsp1407I TGTACA 1 cut(s) 804
Bsp143I GATC 5 cut(s) 132, 612, 682, 1354, 1375
Bsp68I TCGCGA 1 cut(s) 924
BspACI CCGC 5 cut(s) 29, 309, 826, 1256, 1383
BspANI GGCC 2 cut(s) 122, 1046
BspCNI CTCAG 2 cut(s) 29, 733
BspFNI CGCG 4 cut(s) 29, 828, 924, 1385
BspHI TCATGA 2 cut(s) 327, 759
BspLI GGNNCC 7 cut(s) 64, 313, 314, 468, 626, 915, 1134
BspMAI CTGCAG 2 cut(s) 178, 1366
BspMI ACCTGC 2 cut(s) 459, 1338
BspPI GGATC 2 cut(s) 1349, 1370
BspT107I GGYRCC 2 cut(s) 624, 913
BsrDI GCAATG 2 cut(s) 393, 765
BsrGI TGTACA 1 cut(s) 804
BsrI ACTGG 5 cut(s) 358, 850, 914, 1083, 1326
BssECI CCNNGG 2 cut(s) 796, 1009
BssMI GATC 5 cut(s) 132, 612, 682, 1354, 1375
BssNI GRCGYC 2 cut(s) 914, 1269
Bst2UI CCWGG 2 cut(s) 798, 1011
Bst4CI ACNGT 3 cut(s) 48, 629, 730
Bst6I CTCTTC 3 cut(s) 293, 677, 1214
BstACI GRCGYC 2 cut(s) 914, 1269
BstAPI GCANNNNNTGC 1 cut(s) 663
BstAUI TGTACA 1 cut(s) 804
BstC8I GCNNGC 2 cut(s) 225, 603
BstDEI CTNAG 4 cut(s) 16, 100, 435, 720
BstENI CCTNNNNNAGG 1 cut(s) 116
BstF5I GGATG 2 cut(s) 388, 879
BstFNI CGCG 4 cut(s) 29, 828, 924, 1385
BstH2I RGCGCY 1 cut(s) 917
BstHHI GCGC 1 cut(s) 916
BstKTI GATC 5 cut(s) 135, 615, 685, 1357, 1378
BstMAI GTCTC 1 cut(s) 1260
BstMBI GATC 5 cut(s) 132, 612, 682, 1354, 1375
BstMWI GCNNNNNNNGC 3 cut(s) 663, 930, 1043
BstNI CCWGG 2 cut(s) 798, 1011
BstNSI RCATGY 1 cut(s) 805
BstSCI CCNGG 3 cut(s) 796, 957, 1009
BstSFI CTRYAG 2 cut(s) 174, 1362
BstSLI GKGCMC 1 cut(s) 947
BstUI CGCG 4 cut(s) 29, 828, 924, 1385
BstV1I GCAGC 7 cut(s) 58, 647, 653, 1161, 1325, 1376, 1391
BstV2I GAAGAC 3 cut(s) 350, 709, 933
BsuRI GGCC 2 cut(s) 122, 1046
BtgZI GCGATG 1 cut(s) 10
BtsCI GGATG 2 cut(s) 388, 879
BtsIMutI CAGTG 4 cut(s) 351, 726, 843, 1090
BtuMI TCGCGA 1 cut(s) 924
BveI ACCTGC 2 cut(s) 459, 1338
Cac8I GCNNGC 2 cut(s) 225, 603
CaiI CAGNNNCTG 1 cut(s) 698
CciI TCATGA 2 cut(s) 327, 759
CfoI GCGC 1 cut(s) 916
Cfr13I GGNCC 3 cut(s) 312, 1044, 1133
CseI GACGC 1 cut(s) 1277
Csp6I GTAC 5 cut(s) 625, 724, 805, 1243, 1411
CviAII CATG 5 cut(s) 274, 328, 409, 760, 802
CviQI GTAC 5 cut(s) 625, 724, 805, 1243, 1411
DdeI CTNAG 4 cut(s) 16, 100, 435, 720
DinI GGCGCC 1 cut(s) 915
DpnI GATC 5 cut(s) 134, 614, 684, 1356, 1377
DpnII GATC 5 cut(s) 132, 612, 682, 1354, 1375
Eam1104I CTCTTC 3 cut(s) 293, 677, 1214
EarI CTCTTC 3 cut(s) 293, 677, 1214
Ecl136II GAGCTC 1 cut(s) 333
Eco24I GRGCYC 1 cut(s) 335
Eco47I GGWCC 2 cut(s) 312, 1133
Eco53kI GAGCTC 1 cut(s) 333
Eco57I CTGAAG 3 cut(s) 134, 222, 1362
EcoICRI GAGCTC 1 cut(s) 333
EcoNI CCTNNNNNAGG 1 cut(s) 116
EcoO109I RGGNCCY 2 cut(s) 312, 1133
EcoRII CCWGG 2 cut(s) 796, 1009
EcoT38I GRGCYC 1 cut(s) 335
EgeI GGCGCC 1 cut(s) 915
EheI GGCGCC 1 cut(s) 915
Esp3I CGTCTC 1 cut(s) 1260
FaeI CATG 5 cut(s) 277, 331, 412, 763, 805
FaqI GGGAC 2 cut(s) 325, 1322
FatI CATG 5 cut(s) 273, 327, 408, 759, 801
FauI CCCGC 2 cut(s) 302, 833
FbaI TGATCA 1 cut(s) 612
FokI GGATG 2 cut(s) 395, 866
FriOI GRGCYC 1 cut(s) 335
FspBI CTAG 3 cut(s) 686, 975, 1262
GlaI GCGC 1 cut(s) 915
GsuI CTGGAG 3 cut(s) 384, 591, 615
HaeII RGCGCY 1 cut(s) 917
HaeIII GGCC 2 cut(s) 122, 1046
HapII CCGG 2 cut(s) 66, 958
HgaI GACGC 1 cut(s) 1277
HhaI GCGC 1 cut(s) 916
Hin1I GRCGYC 2 cut(s) 914, 1269
Hin1II CATG 5 cut(s) 277, 331, 412, 763, 805
Hin6I GCGC 1 cut(s) 914
HinP1I GCGC 1 cut(s) 914
HincII GTYRAC 4 cut(s) 37, 733, 1000, 1327
HindII GTYRAC 4 cut(s) 37, 733, 1000, 1327
HindIII AAGCTT 1 cut(s) 992
HinfI GANTC 5 cut(s) 21, 546, 820, 1162, 1224
HpaI GTTAAC 2 cut(s) 37, 1327
HpaII CCGG 2 cut(s) 66, 958
HphI GGTGA 2 cut(s) 250, 757
Hpy166II GTNNAC 8 cut(s) 37, 280, 726, 733, 945, 1000, 1120, 1327
Hpy188I TCNGA 4 cut(s) 159, 241, 1140, 1237
Hpy188III TCNNGA 6 cut(s) 18, 328, 686, 760, 923, 1288
Hpy8I GTNNAC 8 cut(s) 37, 280, 726, 733, 945, 1000, 1120, 1327
Hpy99I CGWCG 1 cut(s) 1280
HpyAV CCTTC 3 cut(s) 106, 112, 1337
HpyCH4III ACNGT 3 cut(s) 48, 629, 730
HpyCH4IV ACGT 1 cut(s) 892
HpyCH4V TGCA 8 cut(s) 74, 176, 692, 933, 945, 1201, 1350, 1364
HpyF10VI GCNNNNNNNGC 3 cut(s) 663, 930, 1043
HpyF3I CTNAG 4 cut(s) 16, 100, 435, 720
HpySE526I ACGT 1 cut(s) 892
Hsp92I GRCGYC 2 cut(s) 914, 1269
Hsp92II CATG 5 cut(s) 277, 331, 412, 763, 805
HspAI GCGC 1 cut(s) 914
KasI GGCGCC 1 cut(s) 913
KflI GGGWCCC 1 cut(s) 312
KpnI GGTACC 1 cut(s) 628
Ksp22I TGATCA 1 cut(s) 612
KspAI GTTAAC 2 cut(s) 37, 1327
Kzo9I GATC 5 cut(s) 132, 612, 682, 1354, 1375
LmnI GCTCC 5 cut(s) 68, 338, 403, 695, 1401
Lsp1109I GCAGC 7 cut(s) 58, 647, 653, 1161, 1325, 1376, 1391
LweI GCATC 2 cut(s) 679, 1270
MaeI CTAG 3 cut(s) 686, 975, 1262
MaeII ACGT 1 cut(s) 892
MaeIII GTNAC 2 cut(s) 841, 1391
MalI GATC 5 cut(s) 134, 614, 684, 1356, 1377
MboI GATC 5 cut(s) 132, 612, 682, 1354, 1375
MboII GAAGA 8 cut(s) 127, 280, 350, 664, 714, 938, 1168, 1231
MfeI CAATTG 2 cut(s) 365, 1229
MhlI GDGCHC 3 cut(s) 46, 335, 947
MluCI AATT 6 cut(s) 160, 365, 575, 1229, 1297, 1415
Mly113I GGCGCC 1 cut(s) 914
MlyI GAGTC 2 cut(s) 1156, 1233
MmeI TCCRAC 2 cut(s) 1163, 1403
MseI TTAA 4 cut(s) 36, 56, 462, 1326
MslI CAYNNNNRTG 2 cut(s) 517, 938
MspI CCGG 2 cut(s) 66, 958
MspR9I CCNGG 3 cut(s) 798, 959, 1011
MunI CAATTG 2 cut(s) 365, 1229
Mva1269I GAATGC 1 cut(s) 754
MvaI CCWGG 2 cut(s) 798, 1011
MvnI CGCG 4 cut(s) 29, 828, 924, 1385
MwoI GCNNNNNNNGC 3 cut(s) 663, 930, 1043
NarI GGCGCC 1 cut(s) 914
NciI CCSGG 1 cut(s) 959
NdeII GATC 5 cut(s) 132, 612, 682, 1354, 1375
NlaIII CATG 5 cut(s) 277, 331, 412, 763, 805
NlaIV GGNNCC 7 cut(s) 64, 313, 314, 468, 626, 915, 1134
NmuCI GTSAC 2 cut(s) 841, 1391
NruI TCGCGA 1 cut(s) 924
NspI RCATGY 1 cut(s) 805
PagI TCATGA 2 cut(s) 327, 759
PcsI WCGNNNNNNNCGW 1 cut(s) 1275
PctI GAATGC 1 cut(s) 754
PfeI GAWTC 3 cut(s) 21, 546, 820
PflFI GACNNNGTC 1 cut(s) 986
PleI GAGTC 2 cut(s) 1156, 1232
PluTI GGCGCC 1 cut(s) 917
PpsI GAGTC 2 cut(s) 1156, 1232
PpuMI RGGWCCY 2 cut(s) 312, 1133
Psp124BI GAGCTC 1 cut(s) 335
Psp5II RGGWCCY 2 cut(s) 312, 1133
Psp6I CCWGG 2 cut(s) 796, 1009
PspGI CCWGG 2 cut(s) 796, 1009
PspN4I GGNNCC 7 cut(s) 64, 313, 314, 468, 626, 915, 1134
PspPI GGNCC 3 cut(s) 312, 1044, 1133
PspPPI RGGWCCY 2 cut(s) 312, 1133
PstI CTGCAG 2 cut(s) 178, 1366
PstNI CAGNNNCTG 1 cut(s) 698
PsyI GACNNNGTC 1 cut(s) 986
RruI TCGCGA 1 cut(s) 924
RsaI GTAC 5 cut(s) 626, 725, 806, 1244, 1412
RsaNI GTAC 5 cut(s) 625, 724, 805, 1243, 1411
RseI CAYNNNNRTG 2 cut(s) 517, 938
SacI GAGCTC 1 cut(s) 335
SaqAI TTAA 4 cut(s) 36, 56, 462, 1326
Sau3AI GATC 5 cut(s) 132, 612, 682, 1354, 1375
Sau96I GGNCC 3 cut(s) 312, 1044, 1133
ScaI AGTACT 1 cut(s) 1244
SchI GAGTC 2 cut(s) 1156, 1233
ScrFI CCNGG 3 cut(s) 798, 959, 1011
SduI GDGCHC 3 cut(s) 46, 335, 947
SfaNI GCATC 2 cut(s) 679, 1270
SfcI CTRYAG 2 cut(s) 174, 1362
SfoI GGCGCC 1 cut(s) 915
SinI GGWCC 2 cut(s) 312, 1133
SmiMI CAYNNNNRTG 2 cut(s) 517, 938
SmlI CTYRAG 1 cut(s) 1178
SmoI CTYRAG 1 cut(s) 1178
Sse9I AATT 6 cut(s) 160, 365, 575, 1229, 1297, 1415
SsiI CCGC 5 cut(s) 29, 309, 826, 1256, 1383
SspDI GGCGCC 1 cut(s) 913
SspMI CTAG 3 cut(s) 686, 975, 1262
SstI GAGCTC 1 cut(s) 335
StyD4I CCNGG 3 cut(s) 796, 957, 1009
TaaI ACNGT 3 cut(s) 48, 629, 730
TaiI ACGT 1 cut(s) 895
TasI AATT 6 cut(s) 160, 365, 575, 1229, 1297, 1415
TatI WGTACW 3 cut(s) 723, 804, 1242
TauI GCSGC 2 cut(s) 32, 1259
TfiI GAWTC 3 cut(s) 21, 546, 820
Tru1I TTAA 4 cut(s) 36, 56, 462, 1326
Tru9I TTAA 4 cut(s) 36, 56, 462, 1326
TscAI CASTG 4 cut(s) 358, 733, 850, 1090
TseFI GTSAC 2 cut(s) 841, 1391
TseI GCWGC 8 cut(s) 5, 71, 635, 666, 1174, 1313, 1364, 1404
Tsp45I GTSAC 2 cut(s) 841, 1391
TspDTI ATGAA 6 cut(s) 141, 397, 416, 664, 776, 1077
TspGWI ACGGA 1 cut(s) 872
TspRI CASTG 4 cut(s) 358, 733, 850, 1090
Tth111I GACNNNGTC 1 cut(s) 986
VneI GTGCAC 1 cut(s) 943
VpaK11BI GGWCC 2 cut(s) 312, 1133
XagI CCTNNNNNAGG 1 cut(s) 116
XapI RAATTY 2 cut(s) 160, 1297
XbaI TCTAGA 1 cut(s) 685
XceI RCATGY 1 cut(s) 805
XspI CTAG 3 cut(s) 686, 975, 1262
ZrmI AGTACT 1 cut(s) 1244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.