Rh5BG452000

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000911
Physical Location & Seq
Reverse (-)
72555778 .. 72559284
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG452000.1

Sequence Viewer

Length: 1140 bp
ATGGCTTCCACAGTCTCAACTGTTGGAGCTGTTAGCAGAACACCCTTGAGCTTGAACAGCTCTGGTGCTGGAAGCTCAATCCCAAGCTCAGCTTTCTTGGGCAGCAGTTTGAAGAAGGTCAACAACACAAGACTTCCCTACCAAAAATCTGTATCTGCAGGTAGCTTGAGAATCACAGCAGAAATCGATGAGGGAAAGCAGTCGAAATCAGACAAGTGGAAAGGCCTTGCTTATGACATTTCTGATGACCAACAAGACATTACAAGAGGAAAGGGAATGGTTGATTCTGTCTTCCAAGCTCCCACTGGGACTGGGACTCACAATGCCATCATGAGCTCATATGATTACATCAGCACCGGACTTCGCACGTACAACTTGGATAACAACATGGATGGGTTTTACATAGCTCCAGCTTTCATGGACAAGCTTGTGGTTCATATCACCAAGAACTTCATGAGCCTGCCTAACATTAAGGTTCCCCTTATTCTTGGTATATGGGGAGGAAAAGGTCAGGGAAAATCATTCCAGTGTGAGCTTGTGTTTGCCAAGATGGGAATTACCCCCATCATGATGAGTGCTGGAGAATTAGAAAGTGGGAATGCAGGAGAGCCAGCAAAGTTGATCAGACAAAGGTATCGGGAGGCTTCAGACATCATCCGAAAAGGAAAAATGTGTGCCCTCTTCATCAATGATCTCGATGCAGGTGCTGGTAGGATGGGTGGAACCACACAGTACACTGTGAACAACCAGATGGTCAACGCAACCCTGATGAACATTGCTGATAACCCAACAAATGTGCAGCTTCCCGGTATGTACAACAAGGAGGACAATGCTAGAGTGCCGGTTATAGTCACCGGTAATGACTTCTCTACACTCTATGCGCCTCTTATCCGTGATGGTCGTATGGAGAAGTTTTACTGGGCTCCCACAAGGGAAGACCGGATTGGTGTTTGCATCGGAATCTTCAAGACTGACAATATTCCTGAGGAGGCTGTTGTTAAGATTGTAGACACATTCCCTGGTCAATCTATTGGTAAGCATAGATTAAATATTGAGACAAGTATGAAACAAGAAATTAGAAATGCATTGATTTGTGGAGAGTGTTTGCCATATCATATATATCGATTAACATTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

379

Amino Acids

41.45

Weight (kDa)

8.79

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 165 - 308 4.6e-17 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 311 - 344 6.8e-08 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 692
Acc36I ACCTGC 2 cut(s) 149, 692
AccI GTMKAC 1 cut(s) 1008
AcuI CTGAAG 1 cut(s) 630
AfaI GTAC 3 cut(s) 371, 734, 815
AgeI ACCGGT 1 cut(s) 854
AgsI TTSAA 3 cut(s) 55, 112, 967
AjnI CCWGG 1 cut(s) 1018
AjuI GAANNNNNNNTTGG 2 cut(s) 927, 959
Alw21I GWGCWC 1 cut(s) 338
Alw26I GTCTC 2 cut(s) 19, 1049
AlwNI CAGNNNCTG 1 cut(s) 707
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 2 cut(s) 102, 799
AsiGI ACCGGT 1 cut(s) 854
AspLEI GCGC 1 cut(s) 883
AsuC2I CCSGG 1 cut(s) 807
AsuHPI GGTGA 2 cut(s) 433, 844
AxyI CCTNAGG 1 cut(s) 984
BaeGI GKGCMC 1 cut(s) 679
BaeI ACNNNNGTAYC 2 cut(s) 617, 650
BanII GRGCYC 2 cut(s) 338, 925
BbsI GAAGAC 2 cut(s) 283, 942
Bbv12I GWGCWC 1 cut(s) 338
BbvI GCAGC 2 cut(s) 114, 811
BccI CCATC 7 cut(s) 335, 386, 544, 572, 709, 745, 890
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BciT130I CCWGG 1 cut(s) 1020
BclI TGATCA 1 cut(s) 621
BcnI CCSGG 1 cut(s) 807
BcoDI GTCTC 2 cut(s) 19, 1049
BfaI CTAG 1 cut(s) 834
BfmI CTRYAG 1 cut(s) 156
BfuAI ACCTGC 2 cut(s) 149, 692
BisI GCNGC 2 cut(s) 103, 800
BlpI GCTNAGC 1 cut(s) 88
BlsI GCNGC 2 cut(s) 104, 801
Bme1390I CCNGG 2 cut(s) 807, 1020
BmiI GGNNCC 3 cut(s) 477, 724, 924
BmrFI CCNGG 2 cut(s) 807, 1020
BmrI ACTGGG 3 cut(s) 315, 321, 928
BmsI GCATC 2 cut(s) 688, 963
BmuI ACTGGG 3 cut(s) 315, 321, 928
BpiI GAAGAC 2 cut(s) 283, 942
BpmI CTGGAG 2 cut(s) 393, 600
Bpu1102I GCTNAGC 1 cut(s) 88
BpuEI CTTGAG 2 cut(s) 67, 187
BpuMI CCSGG 1 cut(s) 807
Bsa29I ATCGAT 2 cut(s) 186, 1123
BsaAI YACGTR 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 1018
BsaWI WCCGGW 3 cut(s) 356, 854, 939
Bse118I RCCGGY 2 cut(s) 841, 854
Bse1I ACTGG 4 cut(s) 310, 316, 526, 923
Bse21I CCTNAGG 1 cut(s) 984
Bse3DI GCAATG 1 cut(s) 774
BseBI CCWGG 1 cut(s) 1020
BseCI ATCGAT 2 cut(s) 186, 1123
BseDI CCNNGG 1 cut(s) 1018
BseGI GGATG 3 cut(s) 397, 654, 720
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 2 cut(s) 102, 975
BseNI ACTGG 4 cut(s) 310, 316, 526, 923
BseRI GAGGAG 1 cut(s) 1001
BseSI GKGCMC 1 cut(s) 679
BseXI GCAGC 2 cut(s) 114, 811
BsgI GTGCAG 1 cut(s) 818
BshFI GGCC 1 cut(s) 225
BshTI ACCGGT 1 cut(s) 854
BshVI ATCGAT 2 cut(s) 186, 1123
BsiHKAI GWGCWC 1 cut(s) 338
BsiSI CCGG 5 cut(s) 357, 807, 842, 855, 940
BslFI GGGAC 2 cut(s) 322, 328
BsmAI GTCTC 2 cut(s) 19, 1049
BsmFI GGGAC 2 cut(s) 322, 328
BsmI GAATGC 1 cut(s) 604
BsnI GGCC 1 cut(s) 225
Bsp1286I GDGCHC 3 cut(s) 338, 679, 925
Bsp1407I TGTACA 1 cut(s) 813
Bsp143I GATC 2 cut(s) 621, 691
Bsp1720I GCTNAGC 1 cut(s) 88
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 2 cut(s) 101, 976
BspDI ATCGAT 2 cut(s) 186, 1123
BspHI TCATGA 3 cut(s) 330, 453, 567
BspLI GGNNCC 3 cut(s) 477, 724, 924
BspMAI CTGCAG 1 cut(s) 160
BspMI ACCTGC 2 cut(s) 149, 692
BsrDI GCAATG 1 cut(s) 774
BsrFI RCCGGY 2 cut(s) 841, 854
BsrGI TGTACA 1 cut(s) 813
BsrI ACTGG 4 cut(s) 310, 316, 526, 923
BssAI RCCGGY 2 cut(s) 841, 854
BssECI CCNNGG 1 cut(s) 1018
BssMI GATC 2 cut(s) 621, 691
Bst2UI CCWGG 1 cut(s) 1020
Bst4CI ACNGT 4 cut(s) 13, 22, 732, 739
Bst6I CTCTTC 1 cut(s) 686
BstAUI TGTACA 1 cut(s) 813
BstBAI YACGTR 1 cut(s) 369
BstC8I GCNNGC 2 cut(s) 461, 612
BstDEI CTNAG 2 cut(s) 88, 984
BstF5I GGATG 3 cut(s) 397, 654, 720
BstHHI GCGC 1 cut(s) 883
BstKTI GATC 2 cut(s) 624, 694
BstMAI GTCTC 2 cut(s) 19, 1049
BstMBI GATC 2 cut(s) 621, 691
BstMWI GCNNNNNNNGC 1 cut(s) 57
BstNI CCWGG 1 cut(s) 1020
BstSCI CCNGG 2 cut(s) 805, 1018
BstSFI CTRYAG 1 cut(s) 156
BstSLI GKGCMC 1 cut(s) 679
BstV1I GCAGC 2 cut(s) 114, 811
BstV2I GAAGAC 2 cut(s) 283, 942
Bsu15I ATCGAT 2 cut(s) 186, 1123
Bsu36I CCTNAGG 1 cut(s) 984
BsuRI GGCC 1 cut(s) 225
BsuTUI ATCGAT 2 cut(s) 186, 1123
BtsCI GGATG 3 cut(s) 397, 654, 720
BtsIMutI CAGTG 3 cut(s) 303, 533, 735
BveI ACCTGC 2 cut(s) 149, 692
Cac8I GCNNGC 2 cut(s) 461, 612
CaiI CAGNNNCTG 1 cut(s) 707
CciI TCATGA 3 cut(s) 330, 453, 567
CfoI GCGC 1 cut(s) 883
Cfr10I RCCGGY 2 cut(s) 841, 854
ClaI ATCGAT 2 cut(s) 186, 1123
Csp6I GTAC 3 cut(s) 370, 733, 814
CspAI ACCGGT 1 cut(s) 854
CviAII CATG 6 cut(s) 331, 388, 418, 454, 568, 1137
CviQI GTAC 3 cut(s) 370, 733, 814
DdeI CTNAG 2 cut(s) 88, 984
DpnI GATC 2 cut(s) 623, 693
DpnII GATC 2 cut(s) 621, 691
Eam1104I CTCTTC 1 cut(s) 686
EarI CTCTTC 1 cut(s) 686
Ecl136II GAGCTC 1 cut(s) 336
Eco147I AGGCCT 1 cut(s) 225
Eco24I GRGCYC 2 cut(s) 338, 925
Eco53kI GAGCTC 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 630
Eco81I CCTNAGG 1 cut(s) 984
EcoICRI GAGCTC 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 1018
EcoT22I ATGCAT 1 cut(s) 1087
EcoT38I GRGCYC 2 cut(s) 338, 925
FaeI CATG 6 cut(s) 334, 391, 421, 457, 571, 1140
FalI AAGNNNNNCTT 2 cut(s) 76, 108
FaqI GGGAC 2 cut(s) 322, 328
FatI CATG 6 cut(s) 330, 387, 417, 453, 567, 1136
FauNDI CATATG 1 cut(s) 340
FbaI TGATCA 1 cut(s) 621
FblI GTMKAC 1 cut(s) 1008
Fnu4HI GCNGC 2 cut(s) 103, 800
FokI GGATG 3 cut(s) 404, 641, 727
FriOI GRGCYC 2 cut(s) 338, 925
Fsp4HI GCNGC 2 cut(s) 103, 800
FspBI CTAG 1 cut(s) 834
GlaI GCGC 1 cut(s) 882
GluI GCNGC 2 cut(s) 103, 800
GsuI CTGGAG 2 cut(s) 393, 600
HaeIII GGCC 1 cut(s) 225
HapII CCGG 5 cut(s) 357, 807, 842, 855, 940
HhaI GCGC 1 cut(s) 883
Hin1II CATG 6 cut(s) 334, 391, 421, 457, 571, 1140
Hin6I GCGC 1 cut(s) 881
HinP1I GCGC 1 cut(s) 881
HincII GTYRAC 2 cut(s) 121, 757
HindII GTYRAC 2 cut(s) 121, 757
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 4 cut(s) 171, 284, 316, 960
HpaII CCGG 5 cut(s) 357, 807, 842, 855, 940
HphI GGTGA 2 cut(s) 433, 844
Hpy166II GTNNAC 5 cut(s) 121, 735, 742, 757, 1009
Hpy188I TCNGA 6 cut(s) 211, 244, 626, 649, 659, 959
Hpy188III TCNNGA 7 cut(s) 331, 454, 568, 638, 695, 967, 983
Hpy8I GTNNAC 5 cut(s) 121, 735, 742, 757, 1009
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 4 cut(s) 13, 22, 732, 739
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 6 cut(s) 158, 602, 701, 799, 954, 1085
HpyF10VI GCNNNNNNNGC 1 cut(s) 57
HpyF3I CTNAG 2 cut(s) 88, 984
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 6 cut(s) 334, 391, 421, 457, 571, 1140
HspAI GCGC 1 cut(s) 881
Ksp22I TGATCA 1 cut(s) 621
Kzo9I GATC 2 cut(s) 621, 691
LmnI GCTCC 4 cut(s) 26, 304, 412, 928
Lsp1109I GCAGC 2 cut(s) 114, 811
LweI GCATC 2 cut(s) 688, 963
MaeI CTAG 1 cut(s) 834
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 1 cut(s) 850
MalI GATC 2 cut(s) 623, 693
MboI GATC 2 cut(s) 621, 691
MboII GAAGA 5 cut(s) 124, 283, 673, 947, 955
MhlI GDGCHC 3 cut(s) 338, 679, 925
MluCI AATT 3 cut(s) 555, 584, 1074
MlyI GAGTC 1 cut(s) 310
MmeI TCCRAC 1 cut(s) 4
MnlI CCTC 9 cut(s) 184, 260, 494, 634, 689, 817, 894, 979, 982
Mph1103I ATGCAT 1 cut(s) 1087
MseI TTAA 4 cut(s) 471, 999, 1046, 1127
MslI CAYNNNNRTG 3 cut(s) 526, 569, 1135
MspI CCGG 5 cut(s) 357, 807, 842, 855, 940
MspR9I CCNGG 2 cut(s) 807, 1020
Mva1269I GAATGC 1 cut(s) 604
MvaI CCWGG 1 cut(s) 1020
MwoI GCNNNNNNNGC 1 cut(s) 57
NciI CCSGG 1 cut(s) 807
NdeI CATATG 1 cut(s) 340
NdeII GATC 2 cut(s) 621, 691
NlaIII CATG 6 cut(s) 334, 391, 421, 457, 571, 1140
NlaIV GGNNCC 3 cut(s) 477, 724, 924
NmuCI GTSAC 1 cut(s) 850
NsiI ATGCAT 1 cut(s) 1087
PagI TCATGA 3 cut(s) 330, 453, 567
PaqCI CACCTGC 1 cut(s) 692
PceI AGGCCT 1 cut(s) 225
PctI GAATGC 1 cut(s) 604
PfeI GAWTC 3 cut(s) 171, 284, 960
PinAI ACCGGT 1 cut(s) 854
PkrI GCNGC 2 cut(s) 104, 801
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
Ppu21I YACGTR 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 1018
PspGI CCWGG 1 cut(s) 1018
PspN4I GGNNCC 3 cut(s) 477, 724, 924
PstI CTGCAG 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 707
RsaI GTAC 3 cut(s) 371, 734, 815
RsaNI GTAC 3 cut(s) 370, 733, 814
RseI CAYNNNNRTG 3 cut(s) 526, 569, 1135
SacI GAGCTC 1 cut(s) 338
SaqAI TTAA 4 cut(s) 471, 999, 1046, 1127
SatI GCNGC 2 cut(s) 103, 800
Sau3AI GATC 2 cut(s) 621, 691
SchI GAGTC 1 cut(s) 310
ScrFI CCNGG 2 cut(s) 807, 1020
SduI GDGCHC 3 cut(s) 338, 679, 925
SfaNI GCATC 2 cut(s) 688, 963
SfcI CTRYAG 1 cut(s) 156
SmiMI CAYNNNNRTG 3 cut(s) 526, 569, 1135
SmlI CTYRAG 2 cut(s) 46, 166
SmoI CTYRAG 2 cut(s) 46, 166
Sse9I AATT 3 cut(s) 555, 584, 1074
SseBI AGGCCT 1 cut(s) 225
SspI AATATT 2 cut(s) 979, 1051
SspMI CTAG 1 cut(s) 834
SstI GAGCTC 1 cut(s) 338
StuI AGGCCT 1 cut(s) 225
StyD4I CCNGG 2 cut(s) 805, 1018
TaaI ACNGT 4 cut(s) 13, 22, 732, 739
TaiI ACGT 1 cut(s) 371
TaqI TCGA 4 cut(s) 186, 203, 696, 1123
TasI AATT 3 cut(s) 555, 584, 1074
TatI WGTACW 2 cut(s) 732, 813
TfiI GAWTC 3 cut(s) 171, 284, 960
Tru1I TTAA 4 cut(s) 471, 999, 1046, 1127
Tru9I TTAA 4 cut(s) 471, 999, 1046, 1127
TscAI CASTG 3 cut(s) 310, 533, 742
TseFI GTSAC 1 cut(s) 850
TseI GCWGC 2 cut(s) 102, 799
Tsp45I GTSAC 1 cut(s) 850
TspDTI ATGAA 6 cut(s) 406, 425, 442, 673, 785, 1079
TspGWI ACGGA 1 cut(s) 881
TspRI CASTG 3 cut(s) 310, 533, 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 1008
XspI CTAG 1 cut(s) 834
Zsp2I ATGCAT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.