pycom11g08080

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000911
Physical Location & Seq
Forward (+)
6201361 .. 6203751
2391 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g08080.5

Sequence Viewer

Length: 1041 bp
ATGACCGCCATGCACACAGTCTCGAGCGTCGGAACTGTTAACCGGACGCCGGTGAGCTTGAACGGCTCCGGTTCCGGAAGCTCAGCCTTCTTGGGCACCACTTTGAAGAAAGTGACCTCAAGAGTCCCCTACCAGAAGATTGCATCTGCAGGCAAGTTAAAAATCAATGCGGAAATTGATGAGGATAAGCAAACCAACAAGGACAAATGGAAAGGCCTTGCCTACGATGATTCCGATGACCAACAAGACATAACAAGAGGAAAGGGTTTGGTTGATTCTGTGTTCCAAGCTCCCCAGGGGACTGGAACTCACTATGCTGTCATGAGCTCATACGAATACATCAGTACCGGACTTCGAGAATACAACTTTGACAACAACATGGATGGGTTTTACATCGCTCCAGCTTTCATGGACAAGCTTGTGGTTCACATAACCAAAAACTTCATGAGCCTGCCTAACATTAAGATTCCTCTCATTCTTGGTATATGGGGAGGAAAAGGTCAGGGAAAATCCTTCCAGTGTGAGCTTGTGTTTGCCAAGATGGGAATTACCCCCATCATGATGAGTGCTGGAGAATTAGAAAGCGGGAATGCAGGAGAGCCCGCGAAACTGATCAGGCAAAGGTACCGCGAGGCTGCAGATATCATCAGGAAAGGAAAAATGTGTGCCCTCTTCATCAATGATCTCGATGCAGGTGCCGGTAGGATGGGTGGAACCACACAGTACACTGTGAACAACCAGATGGTGAATGCTACCCTTATGAACATTGCTGATAACCCGACAAACGTGCAACTTCCCGGTATGTACAACAAAGAGGAGAATCCTAGAGTCCCCATCATTGTCACTGGTAACGATTTCTCCACATTGTATGCCCCTCTCATTCGTGACGGTCGTATGGAGAAGTTCTACTGGGCTCCCACTAGGGAAGACCGATCGGTGTTTGCACCGGAATTTTCAAGCCTGACAATGTGTCCCAGGAAGATGTTGTCAAGATTGTTGACACATTCCCCGGTCAATCCATTGATTTCTTCGGTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

347

Amino Acids

38.04

Weight (kDa)

8.92

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 683
Acc36I ACCTGC 1 cut(s) 683
Acc65I GGTACC 1 cut(s) 624
AccB1I GGYRCC 4 cut(s) 95, 624, 695, 1033
AccII CGCG 2 cut(s) 605, 630
AccIII TCCGGA 1 cut(s) 74
AciI CCGC 5 cut(s) 6, 170, 585, 603, 628
AcsI RAATTY 1 cut(s) 950
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 4 cut(s) 346, 626, 725, 806
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 3 cut(s) 61, 106, 957
AhdI GACNNNNNGTC 1 cut(s) 969
AjnI CCWGG 2 cut(s) 294, 974
AluBI AGCT 7 cut(s) 57, 81, 290, 327, 404, 418, 526
AluI AGCT 7 cut(s) 57, 81, 290, 327, 404, 418, 526
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 1 cut(s) 25
Ama87I CYCGRG 1 cut(s) 22
Aor13HI TCCGGA 1 cut(s) 74
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 1 cut(s) 635
ApoI RAATTY 1 cut(s) 950
Asp718I GGTACC 1 cut(s) 624
AsuC2I CCSGG 2 cut(s) 798, 1010
AsuHPI GGTGA 2 cut(s) 64, 757
AvaI CYCGRG 1 cut(s) 22
BaeGI GKGCMC 3 cut(s) 98, 670, 1038
BaeI ACNNNNGTAYC 2 cut(s) 328, 361
BanI GGYRCC 4 cut(s) 95, 624, 695, 1033
BanII GRGCYC 3 cut(s) 329, 603, 916
BbsI GAAGAC 1 cut(s) 933
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 1 cut(s) 622
BccI CCATC 6 cut(s) 377, 535, 563, 700, 736, 842
BceAI ACGGC 1 cut(s) 79
BcgI CGANNNNNNTGC 4 cut(s) 677, 711, 769, 803
BciT130I CCWGG 2 cut(s) 296, 976
BclI TGATCA 1 cut(s) 612
BcnI CCSGG 2 cut(s) 798, 1010
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 2 cut(s) 825, 921
BfmI CTRYAG 2 cut(s) 147, 636
BfuAI ACCTGC 1 cut(s) 683
BisI GCNGC 1 cut(s) 636
BlpI GCTNAGC 1 cut(s) 82
BlsI GCNGC 1 cut(s) 637
Bme1390I CCNGG 4 cut(s) 296, 798, 976, 1010
BmeRI GACNNNNNGTC 1 cut(s) 969
BmeT110I CYCGRG 1 cut(s) 22
BmiI GGNNCC 8 cut(s) 67, 73, 97, 626, 697, 715, 915, 1035
BmrFI CCNGG 4 cut(s) 296, 798, 976, 1010
BmrI ACTGGG 1 cut(s) 919
BmsI GCATC 2 cut(s) 152, 679
BmuI ACTGGG 1 cut(s) 919
BpiI GAAGAC 1 cut(s) 933
BpmI CTGGAG 2 cut(s) 384, 591
Bpu1102I GCTNAGC 1 cut(s) 82
BpuEI CTTGAG 1 cut(s) 103
BpuMI CCSGG 2 cut(s) 798, 1010
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 4 cut(s) 294, 295, 974, 1008
BsaWI WCCGGW 5 cut(s) 42, 68, 74, 347, 946
BsaXI ACNNNNNCTCC 2 cut(s) 842, 872
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse118I RCCGGY 2 cut(s) 49, 698
Bse1I ACTGG 4 cut(s) 307, 517, 850, 914
Bse3DI GCAATG 1 cut(s) 765
BseAI TCCGGA 1 cut(s) 74
BseBI CCWGG 2 cut(s) 296, 976
BseDI CCNNGG 4 cut(s) 294, 295, 974, 1008
BseGI GGATG 2 cut(s) 388, 711
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMI GCAATG 1 cut(s) 765
BseMII CTCAG 1 cut(s) 96
BseNI ACTGG 4 cut(s) 307, 517, 850, 914
BseRI GAGGAG 1 cut(s) 830
BseSI GKGCMC 3 cut(s) 98, 670, 1038
BseXI GCAGC 1 cut(s) 622
Bsh1236I CGCG 2 cut(s) 605, 630
Bsh1285I CGRYCG 2 cut(s) 892, 935
BshFI GGCC 1 cut(s) 216
BshNI GGYRCC 4 cut(s) 95, 624, 695, 1033
BsiEI CGRYCG 2 cut(s) 892, 935
BsiHKAI GWGCWC 1 cut(s) 329
BsiHKCI CYCGRG 1 cut(s) 22
BsiSI CCGG 9 cut(s) 43, 50, 69, 75, 348, 699, 798, 947, 1010
BslFI GGGAC 4 cut(s) 110, 313, 815, 957
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 1 cut(s) 25
BsmFI GGGAC 4 cut(s) 110, 313, 815, 957
BsmI GAATGC 2 cut(s) 595, 754
BsnI GGCC 1 cut(s) 216
BsoBI CYCGRG 1 cut(s) 22
Bsp1286I GDGCHC 6 cut(s) 98, 329, 603, 670, 916, 1038
Bsp13I TCCGGA 1 cut(s) 74
Bsp1407I TGTACA 1 cut(s) 804
Bsp143I GATC 3 cut(s) 612, 682, 932
Bsp1720I GCTNAGC 1 cut(s) 82
BspACI CCGC 5 cut(s) 6, 170, 585, 603, 628
BspANI GGCC 1 cut(s) 216
BspCNI CTCAG 1 cut(s) 95
BspEI TCCGGA 1 cut(s) 74
BspFNI CGCG 2 cut(s) 605, 630
BspHI TCATGA 3 cut(s) 321, 444, 558
BspLI GGNNCC 8 cut(s) 67, 73, 97, 626, 697, 715, 915, 1035
BspMAI CTGCAG 2 cut(s) 151, 640
BspMI ACCTGC 1 cut(s) 683
BspT107I GGYRCC 4 cut(s) 95, 624, 695, 1033
BsrDI GCAATG 1 cut(s) 765
BsrFI RCCGGY 2 cut(s) 49, 698
BsrGI TGTACA 1 cut(s) 804
BsrI ACTGG 4 cut(s) 307, 517, 850, 914
BssAI RCCGGY 2 cut(s) 49, 698
BssECI CCNNGG 4 cut(s) 294, 295, 974, 1008
BssMI GATC 3 cut(s) 612, 682, 932
BssNI GRCGYC 1 cut(s) 47
Bst2UI CCWGG 2 cut(s) 296, 976
Bst4CI ACNGT 5 cut(s) 19, 37, 723, 730, 890
Bst6I CTCTTC 1 cut(s) 677
BstACI GRCGYC 1 cut(s) 47
BstAUI TGTACA 1 cut(s) 804
BstC8I GCNNGC 3 cut(s) 151, 452, 603
BstDEI CTNAG 1 cut(s) 82
BstF5I GGATG 2 cut(s) 388, 711
BstFNI CGCG 2 cut(s) 605, 630
BstKTI GATC 3 cut(s) 615, 685, 935
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 3 cut(s) 612, 682, 932
BstMCI CGRYCG 2 cut(s) 892, 935
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstNI CCWGG 2 cut(s) 296, 976
BstSCI CCNGG 4 cut(s) 294, 796, 974, 1008
BstSFI CTRYAG 2 cut(s) 147, 636
BstSLI GKGCMC 3 cut(s) 98, 670, 1038
BstUI CGCG 2 cut(s) 605, 630
BstV1I GCAGC 1 cut(s) 622
BstV2I GAAGAC 1 cut(s) 933
BstXI CCANNNNNNTGG 1 cut(s) 302
BsuRI GGCC 1 cut(s) 216
BtgZI GCGATG 1 cut(s) 379
BtsCI GGATG 2 cut(s) 388, 711
BtsIMutI CAGTG 3 cut(s) 524, 726, 843
BveI ACCTGC 1 cut(s) 683
Cac8I GCNNGC 3 cut(s) 151, 452, 603
CciI TCATGA 3 cut(s) 321, 444, 558
Cfr10I RCCGGY 2 cut(s) 49, 698
CseI GACGC 2 cut(s) 16, 55
Csp6I GTAC 4 cut(s) 345, 625, 724, 805
CviAII CATG 6 cut(s) 10, 322, 379, 409, 445, 559
CviQI GTAC 4 cut(s) 345, 625, 724, 805
DdeI CTNAG 1 cut(s) 82
DpnI GATC 3 cut(s) 614, 684, 934
DpnII GATC 3 cut(s) 612, 682, 932
DriI GACNNNNNGTC 1 cut(s) 969
Eam1104I CTCTTC 1 cut(s) 677
Eam1105I GACNNNNNGTC 1 cut(s) 969
EarI CTCTTC 1 cut(s) 677
Ecl136II GAGCTC 1 cut(s) 327
Eco147I AGGCCT 1 cut(s) 216
Eco24I GRGCYC 3 cut(s) 329, 603, 916
Eco32I GATATC 1 cut(s) 643
Eco53kI GAGCTC 1 cut(s) 327
Eco88I CYCGRG 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 327
EcoRII CCWGG 2 cut(s) 294, 974
EcoRV GATATC 1 cut(s) 643
EcoT38I GRGCYC 3 cut(s) 329, 603, 916
FaeI CATG 6 cut(s) 13, 325, 382, 412, 448, 562
FaqI GGGAC 4 cut(s) 110, 313, 815, 957
FatI CATG 6 cut(s) 9, 321, 378, 408, 444, 558
FauI CCCGC 2 cut(s) 578, 610
FbaI TGATCA 1 cut(s) 612
Fnu4HI GCNGC 1 cut(s) 636
FokI GGATG 2 cut(s) 395, 718
FriOI GRGCYC 3 cut(s) 329, 603, 916
Fsp4HI GCNGC 1 cut(s) 636
FspBI CTAG 2 cut(s) 825, 921
GluI GCNGC 1 cut(s) 636
GsuI CTGGAG 2 cut(s) 384, 591
HaeIII GGCC 1 cut(s) 216
HapII CCGG 9 cut(s) 43, 50, 69, 75, 348, 699, 798, 947, 1010
HgaI GACGC 2 cut(s) 16, 55
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 6 cut(s) 13, 325, 382, 412, 448, 562
HincII GTYRAC 2 cut(s) 40, 999
HindII GTYRAC 2 cut(s) 40, 999
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 6 cut(s) 123, 230, 275, 466, 820, 828
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 9 cut(s) 43, 50, 69, 75, 348, 699, 798, 947, 1010
HphI GGTGA 2 cut(s) 64, 757
Hpy166II GTNNAC 5 cut(s) 40, 427, 726, 733, 999
Hpy188I TCNGA 2 cut(s) 32, 235
Hpy8I GTNNAC 5 cut(s) 40, 427, 726, 733, 999
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 97, 523
HpyCH4III ACNGT 5 cut(s) 19, 37, 723, 730, 890
HpyCH4IV ACGT 1 cut(s) 786
HpyCH4V TGCA 8 cut(s) 13, 143, 149, 593, 638, 692, 790, 944
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpyF3I CTNAG 1 cut(s) 82
HpySE526I ACGT 1 cut(s) 786
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 6 cut(s) 13, 325, 382, 412, 448, 562
Kpn2I TCCGGA 1 cut(s) 74
KpnI GGTACC 1 cut(s) 628
Ksp22I TGATCA 1 cut(s) 612
KspAI GTTAAC 1 cut(s) 40
Kzo9I GATC 3 cut(s) 612, 682, 932
LmnI GCTCC 4 cut(s) 71, 295, 403, 919
Lsp1109I GCAGC 1 cut(s) 622
LweI GCATC 2 cut(s) 152, 679
MaeI CTAG 2 cut(s) 825, 921
MaeII ACGT 1 cut(s) 786
MaeIII GTNAC 4 cut(s) 112, 841, 848, 884
MalI GATC 3 cut(s) 614, 684, 934
MboI GATC 3 cut(s) 612, 682, 932
MboII GAAGA 6 cut(s) 118, 148, 664, 938, 991, 1020
MhlI GDGCHC 6 cut(s) 98, 329, 603, 670, 916, 1038
MluCI AATT 4 cut(s) 174, 546, 575, 950
MlyI GAGTC 2 cut(s) 132, 837
MmeI TCCRAC 1 cut(s) 10
MnlI CCTC 9 cut(s) 127, 175, 251, 480, 485, 625, 680, 808, 885
MroI TCCGGA 1 cut(s) 74
MseI TTAA 3 cut(s) 39, 158, 462
MslI CAYNNNNRTG 1 cut(s) 560
MspI CCGG 9 cut(s) 43, 50, 69, 75, 348, 699, 798, 947, 1010
MspR9I CCNGG 4 cut(s) 296, 798, 976, 1010
Mva1269I GAATGC 2 cut(s) 595, 754
MvaI CCWGG 2 cut(s) 296, 976
MvnI CGCG 2 cut(s) 605, 630
MwoI GCNNNNNNNGC 1 cut(s) 63
NciI CCSGG 2 cut(s) 798, 1010
NdeII GATC 3 cut(s) 612, 682, 932
NlaIII CATG 6 cut(s) 13, 325, 382, 412, 448, 562
NlaIV GGNNCC 8 cut(s) 67, 73, 97, 626, 697, 715, 915, 1035
NmuCI GTSAC 3 cut(s) 112, 841, 884
PaeR7I CTCGAG 1 cut(s) 22
PagI TCATGA 3 cut(s) 321, 444, 558
PaqCI CACCTGC 1 cut(s) 683
PasI CCCWGGG 1 cut(s) 295
PceI AGGCCT 1 cut(s) 216
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 889
PctI GAATGC 2 cut(s) 595, 754
PfeI GAWTC 4 cut(s) 230, 275, 466, 820
PkrI GCNGC 1 cut(s) 637
Ple19I CGATCG 1 cut(s) 935
PleI GAGTC 2 cut(s) 131, 836
PpsI GAGTC 2 cut(s) 131, 836
Psp124BI GAGCTC 1 cut(s) 329
Psp6I CCWGG 2 cut(s) 294, 974
PspGI CCWGG 2 cut(s) 294, 974
PspN4I GGNNCC 8 cut(s) 67, 73, 97, 626, 697, 715, 915, 1035
PstI CTGCAG 2 cut(s) 151, 640
PvuI CGATCG 1 cut(s) 935
RsaI GTAC 4 cut(s) 346, 626, 725, 806
RsaNI GTAC 4 cut(s) 345, 625, 724, 805
RseI CAYNNNNRTG 1 cut(s) 560
SacI GAGCTC 1 cut(s) 329
SaqAI TTAA 3 cut(s) 39, 158, 462
SatI GCNGC 1 cut(s) 636
Sau3AI GATC 3 cut(s) 612, 682, 932
SchI GAGTC 2 cut(s) 132, 837
ScrFI CCNGG 4 cut(s) 296, 798, 976, 1010
SduI GDGCHC 6 cut(s) 98, 329, 603, 670, 916, 1038
SfaNI GCATC 2 cut(s) 152, 679
SfcI CTRYAG 2 cut(s) 147, 636
Sfr274I CTCGAG 1 cut(s) 22
SgrAI CRCCGGYG 1 cut(s) 49
SlaI CTCGAG 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 560
SmlI CTYRAG 2 cut(s) 22, 118
SmoI CTYRAG 2 cut(s) 22, 118
Sse9I AATT 4 cut(s) 174, 546, 575, 950
SseBI AGGCCT 1 cut(s) 216
SsiI CCGC 5 cut(s) 6, 170, 585, 603, 628
SspMI CTAG 2 cut(s) 825, 921
SstI GAGCTC 1 cut(s) 329
StuI AGGCCT 1 cut(s) 216
StyD4I CCNGG 4 cut(s) 294, 796, 974, 1008
TaaI ACNGT 5 cut(s) 19, 37, 723, 730, 890
TaiI ACGT 1 cut(s) 789
TaqI TCGA 3 cut(s) 23, 355, 687
TaqII GACCGA 1 cut(s) 945
TasI AATT 4 cut(s) 174, 546, 575, 950
TatI WGTACW 2 cut(s) 723, 804
TfiI GAWTC 4 cut(s) 230, 275, 466, 820
Tru1I TTAA 3 cut(s) 39, 158, 462
Tru9I TTAA 3 cut(s) 39, 158, 462
TscAI CASTG 3 cut(s) 524, 733, 850
TseFI GTSAC 3 cut(s) 112, 841, 884
TseI GCWGC 1 cut(s) 635
Tsp45I GTSAC 3 cut(s) 112, 841, 884
TspDTI ATGAA 4 cut(s) 397, 433, 664, 776
TspRI CASTG 3 cut(s) 524, 733, 850
XapI RAATTY 1 cut(s) 950
XhoI CTCGAG 1 cut(s) 22
XspI CTAG 2 cut(s) 825, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.