Rmu_co8504071.1_g000001

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8504071.1
OG0000911
Physical Location & Seq
Reverse (-)
2 .. 2213
2212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8504071.1_g000001.1.cds

Sequence Viewer

Length: 1387 bp
atggctgcttcaatctcaacaaccatcggagctgttaacagagcaccgttgaagttgaatgggtcaggtgcttcatcttcatttccgagttctgctttcttaggcaagaatttgagctccaaagtcacccaaagaaggacttcatctcggaatttcaaagttgctgcagaatacgatgaggaaaagcagacctcaaaggacagatggggaggacttgctttcgacacttcggatgatcagcaggacatcaccagaggaaagggaatggcggacactctgttccaagctccaactggtagtgggactcaccatgccatcatgagctcctacgattacatcagcactggtcttcgccagtacagtttggataacgttatggatggcttctacattgctccagctttcatggataagcttgttgttcacattactaagaacttcttgaacctgccaaacatcaaggttcctcttattttgggtgtttggggaggcaaaggtcaaggaaagtcattccagtgtgagcttgtttttgccaagatgggaatcaaccctattatgatgagtgctggagaactggagagtggaaatgcaggggagccagcaaaactgatcaggcaaaggtatcgtgaagcagcagacatcatcagcaagggcaaaatgtgctgcctcttcattaatgatttggatgcaggagctggaagacttggtggaactactcaatacaccgtcaacaatcaaatggtgaatgctacccttatgaacattgcagacaatccgaccaatgtccaactccctggtatgtacaacaagcaggagaatccccgtgttcccattatagtcaccggaaatgacttctccacattgtatgcccctctcatccgagatggacgtatggagaaattctactgggcgcctactcgtgaagaccgaattggtgtctgcacaggaattttcaagactgataatattcctactgatgacattgtcaagcttgttgacaccttccctggtcagtcaattgacttcttcggtgccctcagggcaagaatttatgatgatgaagtgaggaagtgggttgcaagtgttggagtagaaggtgttgggaagaggcttgttaactcaaaagaaggacccccaactttcaatcagccaaagatgacacttgagaagctcctcgagtatggaaacatgcttgtgcaagagcaggagaatgtgaagagagtccaactgtctgacagatacttgaaagaggcagctctcggagacgccaatgacgatgccatcaagagtggcaacttctatggcaatgcagctcaacagattaaacttcctgtccctgaaggctgtactgatccatctgctgcaaacttcgatcccacagctagga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

462

Amino Acids

50.57

Weight (kDa)

7.55

Isoelectric Point (pI)

33.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 456
AccB1I GGYRCC 2 cut(s) 910, 1031
AciI CCGC 1 cut(s) 269
AclI AACGTT 1 cut(s) 372
AclWI GGATC 2 cut(s) 1346, 1367
AcsI RAATTY 5 cut(s) 109, 151, 899, 948, 1047
AcuI CTGAAG 1 cut(s) 1359
AcyI GRCGYC 2 cut(s) 911, 1266
AfaI GTAC 3 cut(s) 359, 803, 1348
AfiI CCNNNNNNNGG 2 cut(s) 135, 1383
AgsI TTSAA 8 cut(s) 12, 52, 58, 157, 445, 955, 1144, 1246
AjnI CCWGG 2 cut(s) 793, 1006
AjuI GAANNNNNNNTTGG 2 cut(s) 915, 947
AloI GAACNNNNNNTCC 2 cut(s) 263, 295
Alw21I GWGCWC 3 cut(s) 46, 119, 326
Alw26I GTCTC 1 cut(s) 1257
AlwI GGATC 2 cut(s) 1346, 1367
AlwNI CAGNNNCTG 1 cut(s) 695
Ama87I CYCGRG 1 cut(s) 1175
ApeKI GCWGC 7 cut(s) 5, 164, 632, 663, 1253, 1310, 1361
ApoI RAATTY 5 cut(s) 109, 151, 899, 948, 1047
AseI ATTAAT 1 cut(s) 675
Asp700I GAANNNNTTC 1 cut(s) 139
AspLEI GCGC 1 cut(s) 913
AspS9I GGNCC 1 cut(s) 1130
AsuHPI GGTGA 5 cut(s) 118, 241, 299, 754, 832
AvaI CYCGRG 1 cut(s) 1175
AvaII GGWCC 1 cut(s) 1130
AxyI CCTNAGG 1 cut(s) 1037
BaeGI GKGCMC 1 cut(s) 1036
BanI GGYRCC 2 cut(s) 910, 1031
BanII GRGCYC 2 cut(s) 119, 326
BauI CACGAG 1 cut(s) 918
BbsI GAAGAC 3 cut(s) 341, 706, 930
Bbv12I GWGCWC 3 cut(s) 46, 119, 326
BbvI GCAGC 6 cut(s) 151, 644, 650, 1265, 1322, 1348
BccI CCATC 8 cut(s) 32, 198, 323, 374, 532, 878, 1289, 1363
BcgI CGANNNNNNTGC 2 cut(s) 605, 639
BciT130I CCWGG 2 cut(s) 795, 1008
BclI TGATCA 2 cut(s) 235, 609
BcoDI GTCTC 1 cut(s) 1257
BfaI CTAG 1 cut(s) 1383
BfmI CTRYAG 1 cut(s) 165
BfoI RGCGCY 1 cut(s) 914
BfuAI ACCTGC 1 cut(s) 456
BglI GCCNNNNNGGC 1 cut(s) 1040
BisI GCNGC 7 cut(s) 6, 165, 633, 664, 1254, 1311, 1362
BlsI GCNGC 7 cut(s) 7, 166, 634, 665, 1255, 1312, 1363
Bme1390I CCNGG 2 cut(s) 795, 1008
Bme18I GGWCC 1 cut(s) 1130
BmeT110I CYCGRG 1 cut(s) 1175
BmgT120I GGNCC 1 cut(s) 1130
BmiI GGNNCC 5 cut(s) 465, 597, 912, 1033, 1132
BmrFI CCNGG 2 cut(s) 795, 1008
BmrI ACTGGG 1 cut(s) 916
BmsI GCATC 2 cut(s) 676, 1267
BmuI ACTGGG 1 cut(s) 916
BoxI GACNNNNGTC 1 cut(s) 782
BpiI GAAGAC 3 cut(s) 341, 706, 930
BpmI CTGGAG 3 cut(s) 381, 588, 596
BpuEI CTTGAG 1 cut(s) 1184
BsaBI GATNNNNATC 1 cut(s) 542
BsaHI GRCGYC 2 cut(s) 911, 1266
BsaJI CCNNGG 2 cut(s) 793, 1006
BsaWI WCCGGW 1 cut(s) 842
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 1383
Bse1I ACTGG 6 cut(s) 298, 349, 355, 514, 579, 911
Bse21I CCTNAGG 1 cut(s) 1037
Bse3DI GCAATG 3 cut(s) 390, 762, 1312
Bse8I GATNNNNATC 1 cut(s) 542
BseBI CCWGG 2 cut(s) 795, 1008
BseDI CCNNGG 2 cut(s) 793, 1006
BseGI GGATG 4 cut(s) 238, 385, 691, 876
BseJI GATNNNNATC 1 cut(s) 542
BseLI CCNNNNNNNGG 2 cut(s) 135, 1383
BseMI GCAATG 3 cut(s) 390, 762, 1312
BseMII CTCAG 1 cut(s) 1051
BseNI ACTGG 6 cut(s) 298, 349, 355, 514, 579, 911
BseRI GAGGAG 1 cut(s) 1163
BseSI GKGCMC 1 cut(s) 1036
BseXI GCAGC 6 cut(s) 151, 644, 650, 1265, 1322, 1348
BsgI GTGCAG 1 cut(s) 925
BshNI GGYRCC 2 cut(s) 910, 1031
BsiHKAI GWGCWC 3 cut(s) 46, 119, 326
BsiHKCI CYCGRG 1 cut(s) 1175
BsiSI CCGG 1 cut(s) 843
BslFI GGGAC 2 cut(s) 316, 1319
BslI CCNNNNNNNGG 2 cut(s) 135, 1383
BsmAI GTCTC 1 cut(s) 1257
BsmBI CGTCTC 1 cut(s) 1257
BsmFI GGGAC 2 cut(s) 316, 1319
BsmI GAATGC 1 cut(s) 751
BsoBI CYCGRG 1 cut(s) 1175
Bsp1286I GDGCHC 4 cut(s) 46, 119, 326, 1036
Bsp1407I TGTACA 1 cut(s) 801
Bsp143I GATC 4 cut(s) 235, 609, 1351, 1372
BspACI CCGC 1 cut(s) 269
BspCNI CTCAG 1 cut(s) 1050
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 5 cut(s) 465, 597, 912, 1033, 1132
BspMAI CTGCAG 1 cut(s) 169
BspMI ACCTGC 1 cut(s) 456
BspPI GGATC 2 cut(s) 1346, 1367
BspT107I GGYRCC 2 cut(s) 910, 1031
BsrDI GCAATG 3 cut(s) 390, 762, 1312
BsrGI TGTACA 1 cut(s) 801
BsrI ACTGG 6 cut(s) 298, 349, 355, 514, 579, 911
BssECI CCNNGG 2 cut(s) 793, 1006
BssMI GATC 4 cut(s) 235, 609, 1351, 1372
BssNI GRCGYC 2 cut(s) 911, 1266
BssSI CACGAG 1 cut(s) 918
Bst2BI CACGAG 1 cut(s) 918
Bst2UI CCWGG 2 cut(s) 795, 1008
Bst4CI ACNGT 4 cut(s) 48, 362, 727, 1230
Bst6I CTCTTC 3 cut(s) 674, 1100, 1211
BstACI GRCGYC 2 cut(s) 911, 1266
BstAPI GCANNNNNTGC 1 cut(s) 660
BstAUI TGTACA 1 cut(s) 801
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 3 cut(s) 100, 432, 1037
BstF5I GGATG 4 cut(s) 238, 385, 691, 876
BstH2I RGCGCY 1 cut(s) 914
BstHHI GCGC 1 cut(s) 913
BstKTI GATC 4 cut(s) 238, 612, 1354, 1375
BstMAI GTCTC 1 cut(s) 1257
BstMBI GATC 4 cut(s) 235, 609, 1351, 1372
BstMWI GCNNNNNNNGC 2 cut(s) 660, 1040
BstNI CCWGG 2 cut(s) 795, 1008
BstNSI RCATGY 1 cut(s) 1192
BstPAI GACNNNNGTC 1 cut(s) 782
BstSCI CCNGG 2 cut(s) 793, 1006
BstSFI CTRYAG 1 cut(s) 165
BstSLI GKGCMC 1 cut(s) 1036
BstV1I GCAGC 6 cut(s) 151, 644, 650, 1265, 1322, 1348
BstV2I GAAGAC 3 cut(s) 341, 706, 930
BstXI CCANNNNNNTGG 1 cut(s) 794
Bsu36I CCTNAGG 1 cut(s) 1037
BtsCI GGATG 4 cut(s) 238, 385, 691, 876
BtsIMutI CAGTG 2 cut(s) 342, 521
BveI ACCTGC 1 cut(s) 456
Cac8I GCNNGC 1 cut(s) 600
CaiI CAGNNNCTG 1 cut(s) 695
CciI TCATGA 1 cut(s) 318
CfoI GCGC 1 cut(s) 913
Cfr13I GGNCC 1 cut(s) 1130
CseI GACGC 1 cut(s) 1274
Csp6I GTAC 3 cut(s) 358, 802, 1347
CviAII CATG 4 cut(s) 311, 319, 406, 1189
CviQI GTAC 3 cut(s) 358, 802, 1347
DdeI CTNAG 3 cut(s) 100, 432, 1037
DinI GGCGCC 1 cut(s) 912
DpnI GATC 4 cut(s) 237, 611, 1353, 1374
DpnII GATC 4 cut(s) 235, 609, 1351, 1372
Eam1104I CTCTTC 3 cut(s) 674, 1100, 1211
EarI CTCTTC 3 cut(s) 674, 1100, 1211
EciI GGCGGA 1 cut(s) 284
Ecl136II GAGCTC 2 cut(s) 117, 324
Eco24I GRGCYC 2 cut(s) 119, 326
Eco47I GGWCC 1 cut(s) 1130
Eco53kI GAGCTC 2 cut(s) 117, 324
Eco57I CTGAAG 1 cut(s) 1359
Eco81I CCTNAGG 1 cut(s) 1037
Eco88I CYCGRG 1 cut(s) 1175
EcoICRI GAGCTC 2 cut(s) 117, 324
EcoO109I RGGNCCY 1 cut(s) 1130
EcoRII CCWGG 2 cut(s) 793, 1006
EcoT38I GRGCYC 2 cut(s) 119, 326
EgeI GGCGCC 1 cut(s) 912
EheI GGCGCC 1 cut(s) 912
Esp3I CGTCTC 1 cut(s) 1257
FaeI CATG 4 cut(s) 314, 322, 409, 1192
FalI AAGNNNNNCTT 4 cut(s) 124, 156, 425, 457
FaqI GGGAC 2 cut(s) 316, 1319
FatI CATG 4 cut(s) 310, 318, 405, 1188
FbaI TGATCA 2 cut(s) 235, 609
Fnu4HI GCNGC 7 cut(s) 6, 165, 633, 664, 1254, 1311, 1362
FokI GGATG 4 cut(s) 245, 392, 698, 863
FriOI GRGCYC 2 cut(s) 119, 326
Fsp4HI GCNGC 7 cut(s) 6, 165, 633, 664, 1254, 1311, 1362
FspBI CTAG 1 cut(s) 1383
GlaI GCGC 1 cut(s) 912
GluI GCNGC 7 cut(s) 6, 165, 633, 664, 1254, 1311, 1362
GsuI CTGGAG 3 cut(s) 381, 588, 596
HaeII RGCGCY 1 cut(s) 914
HapII CCGG 1 cut(s) 843
HgaI GACGC 1 cut(s) 1274
HhaI GCGC 1 cut(s) 913
Hin1I GRCGYC 2 cut(s) 911, 1266
Hin1II CATG 4 cut(s) 314, 322, 409, 1192
Hin6I GCGC 1 cut(s) 911
HinP1I GCGC 1 cut(s) 911
HincII GTYRAC 4 cut(s) 37, 730, 997, 1117
HindII GTYRAC 4 cut(s) 37, 730, 997, 1117
HindIII AAGCTT 2 cut(s) 413, 989
HinfI GANTC 4 cut(s) 304, 543, 817, 1221
HpaI GTTAAC 2 cut(s) 37, 1117
HpaII CCGG 1 cut(s) 843
HphI GGTGA 5 cut(s) 118, 241, 299, 754, 832
Hpy166II GTNNAC 5 cut(s) 37, 424, 730, 997, 1117
Hpy188I TCNGA 8 cut(s) 29, 87, 150, 232, 777, 881, 1234, 1262
Hpy188III TCNNGA 6 cut(s) 319, 442, 626, 920, 955, 1285
Hpy8I GTNNAC 5 cut(s) 37, 424, 730, 997, 1117
HpyAV CCTTC 5 cut(s) 129, 1012, 1088, 1121, 1334
HpyCH4III ACNGT 4 cut(s) 48, 362, 727, 1230
HpyCH4IV ACGT 2 cut(s) 372, 889
HpyCH4V TGCA 9 cut(s) 167, 590, 689, 767, 942, 1079, 1198, 1310, 1364
HpyF10VI GCNNNNNNNGC 2 cut(s) 660, 1040
HpyF3I CTNAG 3 cut(s) 100, 432, 1037
HpySE526I ACGT 2 cut(s) 372, 889
Hsp92I GRCGYC 2 cut(s) 911, 1266
Hsp92II CATG 4 cut(s) 314, 322, 409, 1192
HspAI GCGC 1 cut(s) 911
KasI GGCGCC 1 cut(s) 910
Ksp22I TGATCA 2 cut(s) 235, 609
KspAI GTTAAC 2 cut(s) 37, 1117
Kzo9I GATC 4 cut(s) 235, 609, 1351, 1372
LmnI GCTCC 8 cut(s) 29, 122, 292, 329, 400, 595, 692, 1176
Lsp1109I GCAGC 6 cut(s) 151, 644, 650, 1265, 1322, 1348
LweI GCATC 2 cut(s) 676, 1267
MaeI CTAG 1 cut(s) 1383
MaeII ACGT 2 cut(s) 372, 889
MaeIII GTNAC 2 cut(s) 124, 838
MalI GATC 4 cut(s) 237, 611, 1353, 1374
MboI GATC 4 cut(s) 235, 609, 1351, 1372
MboII GAAGA 8 cut(s) 69, 341, 661, 711, 935, 1018, 1117, 1228
MfeI CAATTG 1 cut(s) 1017
MhlI GDGCHC 4 cut(s) 46, 119, 326, 1036
MluCI AATT 7 cut(s) 109, 151, 899, 930, 948, 1017, 1047
Mly113I GGCGCC 1 cut(s) 911
MlyI GAGTC 2 cut(s) 298, 1230
MmeI TCCRAC 5 cut(s) 314, 800, 811, 1066, 1249
MroXI GAANNNNTTC 1 cut(s) 139
MseI TTAA 4 cut(s) 36, 675, 1116, 1323
MslI CAYNNNNRTG 2 cut(s) 514, 1193
MspI CCGG 1 cut(s) 843
MspR9I CCNGG 2 cut(s) 795, 1008
MunI CAATTG 1 cut(s) 1017
Mva1269I GAATGC 1 cut(s) 751
MvaI CCWGG 2 cut(s) 795, 1008
MwoI GCNNNNNNNGC 2 cut(s) 660, 1040
NarI GGCGCC 1 cut(s) 911
NdeII GATC 4 cut(s) 235, 609, 1351, 1372
NlaIII CATG 4 cut(s) 314, 322, 409, 1192
NlaIV GGNNCC 5 cut(s) 465, 597, 912, 1033, 1132
NmuCI GTSAC 2 cut(s) 124, 838
NspI RCATGY 1 cut(s) 1192
PaeR7I CTCGAG 1 cut(s) 1175
PagI TCATGA 1 cut(s) 318
PcsI WCGNNNNNNNCGW 2 cut(s) 925, 1272
PctI GAATGC 1 cut(s) 751
PdmI GAANNNNTTC 1 cut(s) 139
PfeI GAWTC 2 cut(s) 543, 817
PflFI GACNNNGTC 1 cut(s) 983
PkrI GCNGC 7 cut(s) 7, 166, 634, 665, 1255, 1312, 1363
PleI GAGTC 2 cut(s) 298, 1229
PluTI GGCGCC 1 cut(s) 914
PpsI GAGTC 2 cut(s) 298, 1229
PpuMI RGGWCCY 1 cut(s) 1130
PshAI GACNNNNGTC 1 cut(s) 782
PshBI ATTAAT 1 cut(s) 675
Psp124BI GAGCTC 2 cut(s) 119, 326
Psp1406I AACGTT 1 cut(s) 372
Psp5II RGGWCCY 1 cut(s) 1130
Psp6I CCWGG 2 cut(s) 793, 1006
PspGI CCWGG 2 cut(s) 793, 1006
PspN4I GGNNCC 5 cut(s) 465, 597, 912, 1033, 1132
PspPI GGNCC 1 cut(s) 1130
PspPPI RGGWCCY 1 cut(s) 1130
PspXI VCTCGAGB 1 cut(s) 1175
PstI CTGCAG 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 695
PsyI GACNNNGTC 1 cut(s) 983
RsaI GTAC 3 cut(s) 359, 803, 1348
RsaNI GTAC 3 cut(s) 358, 802, 1347
RseI CAYNNNNRTG 2 cut(s) 514, 1193
SacI GAGCTC 2 cut(s) 119, 326
SaqAI TTAA 4 cut(s) 36, 675, 1116, 1323
SatI GCNGC 7 cut(s) 6, 165, 633, 664, 1254, 1311, 1362
Sau3AI GATC 4 cut(s) 235, 609, 1351, 1372
Sau96I GGNCC 1 cut(s) 1130
SchI GAGTC 2 cut(s) 298, 1230
ScrFI CCNGG 2 cut(s) 795, 1008
SduI GDGCHC 4 cut(s) 46, 119, 326, 1036
SfaNI GCATC 2 cut(s) 676, 1267
SfcI CTRYAG 1 cut(s) 165
SfoI GGCGCC 1 cut(s) 912
Sfr274I CTCGAG 1 cut(s) 1175
SinI GGWCC 1 cut(s) 1130
SlaI CTCGAG 1 cut(s) 1175
SmiMI CAYNNNNRTG 2 cut(s) 514, 1193
SmlI CTYRAG 2 cut(s) 1163, 1175
SmoI CTYRAG 2 cut(s) 1163, 1175
Sse9I AATT 7 cut(s) 109, 151, 899, 930, 948, 1017, 1047
SsiI CCGC 1 cut(s) 269
SspDI GGCGCC 1 cut(s) 910
SspI AATATT 1 cut(s) 967
SspMI CTAG 1 cut(s) 1383
SstI GAGCTC 2 cut(s) 119, 326
StyD4I CCNGG 2 cut(s) 793, 1006
TaaI ACNGT 4 cut(s) 48, 362, 727, 1230
TaiI ACGT 2 cut(s) 375, 892
TaqI TCGA 3 cut(s) 222, 1176, 1371
TaqII GACCGA 1 cut(s) 942
TasI AATT 7 cut(s) 109, 151, 899, 930, 948, 1017, 1047
TatI WGTACW 3 cut(s) 357, 801, 1346
TfiI GAWTC 2 cut(s) 543, 817
Tru1I TTAA 4 cut(s) 36, 675, 1116, 1323
Tru9I TTAA 4 cut(s) 36, 675, 1116, 1323
TscAI CASTG 2 cut(s) 349, 521
TseFI GTSAC 2 cut(s) 124, 838
TseI GCWGC 7 cut(s) 5, 164, 632, 663, 1253, 1310, 1361
Tsp45I GTSAC 2 cut(s) 124, 838
TspDTI ATGAA 7 cut(s) 63, 69, 132, 394, 661, 773, 1074
TspRI CASTG 2 cut(s) 349, 521
Tth111I GACNNNGTC 1 cut(s) 983
VpaK11BI GGWCC 1 cut(s) 1130
VspI ATTAAT 1 cut(s) 675
XapI RAATTY 5 cut(s) 109, 151, 899, 948, 1047
XceI RCATGY 1 cut(s) 1192
XcmI CCANNNNNNNNNTGG 1 cut(s) 290
XhoI CTCGAG 1 cut(s) 1175
XmnI GAANNNNTTC 1 cut(s) 139
XspI CTAG 1 cut(s) 1383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.