pycom07g18940

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000911
Physical Location & Seq
Reverse (-)
21285515 .. 21286403
889 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g18940.3

Sequence Viewer

Length: 756 bp
ATGATGAGTCTGATCAGGCAAAGGTACCGTGAAGCGGCCGACATTATCAAGAAGGGTAAAATGTGCGCACTCTTCATCAACGATCTTGATGCAGGAGCTGGTCAGCTTGGTGGAACCACCCAATACACGGTCAACAACCAAATGGTTAATGCCACCCTCATGAACATTGCTGATAACCCGACAAACGTCCAGCTCCCGGGTATGTCCAACAAGGAGGAGAACCCCCGTGTCCCAATCATTGTCACCAGTAACGGTTTCTCAACATTGTACGCTCCTCTCATCCGTGACGGCCGTATGGAGAAGTTCTACTGGGCTCCCACCCGCGAAGATTGCATTGGAGTCTGCATTGGGATCTTCAGGAGTGACAATGTTGCTAAGGAAGACATTGTCAAGATTGTTGACACCATCCCGGGCCAATCTATTGGTATGCTAAATTCAGACATTGTATGTCATGTCATCATACAATTTGACATGTTATGTTATCAGATAGTCATTCTGACCCTCTGTCATGATTTTGCAGATTTCTTTGGTGCCTCGAGGGCTAGGGTTTACGATGATGAAGTGAGGAAGTGGATTTCGGGTGTTGGCGTGGAGGGCATTGGGAAGAAGCTTCCGAAGATGACAATCGAAAAGCTCCTCGAGTACGGAAACATGCTTATCCAAGAGCAAGAGAATGTGAAGAGAGTTCAATTGGCTGATAAGTACTTGAGCGAGGCTGCTCTTGGTGATGCCAACTCAGATGCTATGGACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

252

Amino Acids

28.01

Weight (kDa)

5.19

Isoelectric Point (pI)

27.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 535
Acc16I TGCGCA 1 cut(s) 67
Acc65I GGTACC 1 cut(s) 24
AccB1I GGYRCC 2 cut(s) 24, 530
AccII CGCG 1 cut(s) 324
AciI CCGC 2 cut(s) 35, 322
AclWI GGATC 1 cut(s) 359
AcoI YGGCCR 2 cut(s) 36, 289
AcsI RAATTY 1 cut(s) 433
AcuI CTGAAG 1 cut(s) 340
AfaI GTAC 4 cut(s) 26, 269, 644, 704
AfiI CCNNNNNNNGG 3 cut(s) 34, 127, 196
AflIII ACRYGT 1 cut(s) 471
AgsI TTSAA 1 cut(s) 689
AhdI GACNNNNNGTC 1 cut(s) 504
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 5 cut(s) 98, 106, 193, 610, 634
AluI AGCT 5 cut(s) 98, 106, 193, 610, 634
AlwI GGATC 1 cut(s) 359
AlwNI CAGNNNCTG 1 cut(s) 98
Ama87I CYCGRG 4 cut(s) 196, 409, 535, 638
AoxI GGCC 3 cut(s) 36, 289, 412
ApeKI GCWGC 1 cut(s) 716
ApoI RAATTY 1 cut(s) 433
Asp718I GGTACC 1 cut(s) 24
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 1 cut(s) 412
AsuC2I CCSGG 4 cut(s) 197, 198, 410, 411
AsuHPI GGTGA 2 cut(s) 235, 737
AvaI CYCGRG 4 cut(s) 196, 409, 535, 638
BanI GGYRCC 2 cut(s) 24, 530
BanII GRGCYC 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 703
BccI CCATC 1 cut(s) 413
BceAI ACGGC 2 cut(s) 276, 304
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BclI TGATCA 1 cut(s) 12
BcnI CCSGG 4 cut(s) 197, 198, 410, 411
BfaI CTAG 1 cut(s) 543
BglI GCCNNNNNGGC 1 cut(s) 539
BisI GCNGC 2 cut(s) 36, 717
BlsI GCNGC 2 cut(s) 37, 718
BmcAI AGTACT 1 cut(s) 704
Bme1390I CCNGG 4 cut(s) 197, 198, 410, 411
BmeRI GACNNNNNGTC 1 cut(s) 504
BmeT110I CYCGRG 4 cut(s) 196, 409, 535, 638
BmgT120I GGNCC 1 cut(s) 412
BmiI GGNNCC 4 cut(s) 26, 115, 315, 532
BmrFI CCNGG 4 cut(s) 197, 198, 410, 411
BmrI ACTGGG 1 cut(s) 319
BmsI GCATC 3 cut(s) 79, 718, 730
BmuI ACTGGG 1 cut(s) 319
BoxI GACNNNNGTC 1 cut(s) 185
BpiI GAAGAC 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 727
BpuMI CCSGG 4 cut(s) 197, 198, 410, 411
BsaBI GATNNNNATC 1 cut(s) 623
BsaJI CCNNGG 2 cut(s) 196, 409
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 127, 196
Bse1I ACTGG 2 cut(s) 246, 314
Bse3DI GCAATG 1 cut(s) 165
Bse8I GATNNNNATC 1 cut(s) 623
BseDI CCNNGG 2 cut(s) 196, 409
BseGI GGATG 2 cut(s) 279, 405
BseJI GATNNNNATC 1 cut(s) 623
BseLI CCNNNNNNNGG 3 cut(s) 34, 127, 196
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 1 cut(s) 750
BseNI ACTGG 2 cut(s) 246, 314
BseRI GAGGAG 3 cut(s) 230, 264, 626
BseX3I CGGCCG 2 cut(s) 36, 289
BseXI GCAGC 1 cut(s) 703
Bsh1236I CGCG 1 cut(s) 324
Bsh1285I CGRYCG 2 cut(s) 39, 292
BshFI GGCC 3 cut(s) 38, 291, 414
BshNI GGYRCC 2 cut(s) 24, 530
BsiEI CGRYCG 2 cut(s) 39, 292
BsiHKCI CYCGRG 4 cut(s) 196, 409, 535, 638
BsiSI CCGG 2 cut(s) 197, 410
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 3 cut(s) 34, 127, 196
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 3 cut(s) 38, 291, 414
BsoBI CYCGRG 4 cut(s) 196, 409, 535, 638
Bsp1286I GDGCHC 1 cut(s) 316
Bsp143I GATC 3 cut(s) 12, 82, 351
BspACI CCGC 2 cut(s) 35, 322
BspANI GGCC 3 cut(s) 38, 291, 414
BspCNI CTCAG 1 cut(s) 749
BspFNI CGCG 1 cut(s) 324
BspHI TCATGA 2 cut(s) 159, 508
BspLI GGNNCC 4 cut(s) 26, 115, 315, 532
BspPI GGATC 1 cut(s) 359
BspT107I GGYRCC 2 cut(s) 24, 530
BsrDI GCAATG 1 cut(s) 165
BsrI ACTGG 2 cut(s) 246, 314
BssECI CCNNGG 2 cut(s) 196, 409
BssMI GATC 3 cut(s) 12, 82, 351
Bst4CI ACNGT 3 cut(s) 29, 130, 254
Bst6I CTCTTC 2 cut(s) 77, 674
BstDEI CTNAG 2 cut(s) 375, 736
BstF5I GGATG 2 cut(s) 279, 405
BstFNI CGCG 1 cut(s) 324
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 3 cut(s) 15, 85, 354
BstMBI GATC 3 cut(s) 12, 82, 351
BstMCI CGRYCG 2 cut(s) 39, 292
BstMWI GCNNNNNNNGC 3 cut(s) 330, 539, 594
BstNSI RCATGY 2 cut(s) 475, 655
BstPAI GACNNNNGTC 1 cut(s) 185
BstSCI CCNGG 4 cut(s) 195, 196, 408, 409
BstUI CGCG 1 cut(s) 324
BstV1I GCAGC 1 cut(s) 703
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 1 cut(s) 351
BstXI CCANNNNNNTGG 1 cut(s) 422
BstYI RGATCY 1 cut(s) 351
BstZI CGGCCG 2 cut(s) 36, 289
BsuRI GGCC 3 cut(s) 38, 291, 414
BtsCI GGATG 2 cut(s) 279, 405
CaiI CAGNNNCTG 1 cut(s) 98
CciI TCATGA 2 cut(s) 159, 508
CfoI GCGC 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 412
Cfr9I CCCGGG 2 cut(s) 196, 409
Csp6I GTAC 4 cut(s) 25, 268, 643, 703
CviAII CATG 5 cut(s) 160, 452, 472, 509, 652
CviQI GTAC 4 cut(s) 25, 268, 643, 703
DdeI CTNAG 2 cut(s) 375, 736
DpnI GATC 3 cut(s) 14, 84, 353
DpnII GATC 3 cut(s) 12, 82, 351
DriI GACNNNNNGTC 1 cut(s) 504
EaeI YGGCCR 2 cut(s) 36, 289
EagI CGGCCG 2 cut(s) 36, 289
Eam1104I CTCTTC 2 cut(s) 77, 674
Eam1105I GACNNNNNGTC 1 cut(s) 504
EarI CTCTTC 2 cut(s) 77, 674
EclXI CGGCCG 2 cut(s) 36, 289
Eco24I GRGCYC 1 cut(s) 316
Eco52I CGGCCG 2 cut(s) 36, 289
Eco57I CTGAAG 1 cut(s) 340
Eco88I CYCGRG 4 cut(s) 196, 409, 535, 638
EcoT38I GRGCYC 1 cut(s) 316
FaeI CATG 5 cut(s) 163, 455, 475, 512, 655
FaqI GGGAC 1 cut(s) 215
FatI CATG 5 cut(s) 159, 451, 471, 508, 651
FauI CCCGC 1 cut(s) 329
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 36, 717
FokI GGATG 2 cut(s) 266, 392
FriOI GRGCYC 1 cut(s) 316
Fsp4HI GCNGC 2 cut(s) 36, 717
FspAI RTGCGCAY 1 cut(s) 67
FspBI CTAG 1 cut(s) 543
FspI TGCGCA 1 cut(s) 67
GlaI GCGC 1 cut(s) 67
GluI GCNGC 2 cut(s) 36, 717
HaeIII GGCC 3 cut(s) 38, 291, 414
HapII CCGG 2 cut(s) 197, 410
HhaI GCGC 1 cut(s) 68
Hin1II CATG 5 cut(s) 163, 455, 475, 512, 655
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 2 cut(s) 133, 400
HindII GTYRAC 2 cut(s) 133, 400
HindIII AAGCTT 1 cut(s) 608
HinfI GANTC 2 cut(s) 7, 339
HpaII CCGG 2 cut(s) 197, 410
HphI GGTGA 2 cut(s) 235, 737
Hpy166II GTNNAC 3 cut(s) 133, 400, 550
Hpy188I TCNGA 6 cut(s) 12, 439, 486, 498, 615, 739
Hpy188III TCNNGA 6 cut(s) 49, 86, 160, 358, 391, 509
Hpy8I GTNNAC 3 cut(s) 133, 400, 550
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 3 cut(s) 29, 130, 254
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 4 cut(s) 92, 333, 345, 518
HpyF10VI GCNNNNNNNGC 3 cut(s) 330, 539, 594
HpyF3I CTNAG 2 cut(s) 375, 736
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 5 cut(s) 163, 455, 475, 512, 655
HspAI GCGC 1 cut(s) 66
KpnI GGTACC 1 cut(s) 28
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 3 cut(s) 12, 82, 351
LmnI GCTCC 5 cut(s) 95, 198, 277, 319, 639
LpnPI CCDG 8 cut(s) 78, 84, 203, 210, 259, 295, 343, 423
Lsp1109I GCAGC 1 cut(s) 703
LweI GCATC 3 cut(s) 79, 718, 730
MaeI CTAG 1 cut(s) 543
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 4 cut(s) 241, 248, 284, 362
MalI GATC 3 cut(s) 14, 84, 353
MboI GATC 3 cut(s) 12, 82, 351
MboII GAAGA 7 cut(s) 64, 338, 346, 392, 616, 628, 691
MfeI CAATTG 1 cut(s) 689
MflI RGATCY 1 cut(s) 351
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 3 cut(s) 433, 464, 689
MlyI GAGTC 2 cut(s) 16, 348
MmeI TCCRAC 1 cut(s) 231
MseI TTAA 1 cut(s) 147
MslI CAYNNNNRTG 1 cut(s) 158
MspI CCGG 2 cut(s) 197, 410
MspR9I CCNGG 4 cut(s) 197, 198, 410, 411
MunI CAATTG 1 cut(s) 689
MvnI CGCG 1 cut(s) 324
MwoI GCNNNNNNNGC 3 cut(s) 330, 539, 594
NciI CCSGG 4 cut(s) 197, 198, 410, 411
NdeII GATC 3 cut(s) 12, 82, 351
NlaIII CATG 5 cut(s) 163, 455, 475, 512, 655
NlaIV GGNNCC 4 cut(s) 26, 115, 315, 532
NmuCI GTSAC 3 cut(s) 241, 284, 362
NsbI TGCGCA 1 cut(s) 67
NspI RCATGY 2 cut(s) 475, 655
PaeR7I CTCGAG 2 cut(s) 535, 638
PagI TCATGA 2 cut(s) 159, 508
PciI ACATGT 1 cut(s) 471
PflFI GACNNNGTC 1 cut(s) 386
PkrI GCNGC 2 cut(s) 37, 718
PleI GAGTC 2 cut(s) 15, 347
PpsI GAGTC 2 cut(s) 15, 347
PscI ACATGT 1 cut(s) 471
PshAI GACNNNNGTC 1 cut(s) 185
PspN4I GGNNCC 4 cut(s) 26, 115, 315, 532
PspPI GGNCC 1 cut(s) 412
PspXI VCTCGAGB 2 cut(s) 535, 638
PstNI CAGNNNCTG 1 cut(s) 98
PsuI RGATCY 1 cut(s) 351
PsyI GACNNNGTC 1 cut(s) 386
RsaI GTAC 4 cut(s) 26, 269, 644, 704
RsaNI GTAC 4 cut(s) 25, 268, 643, 703
RseI CAYNNNNRTG 1 cut(s) 158
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 2 cut(s) 36, 717
Sau3AI GATC 3 cut(s) 12, 82, 351
Sau96I GGNCC 1 cut(s) 412
ScaI AGTACT 1 cut(s) 704
SchI GAGTC 2 cut(s) 16, 348
ScrFI CCNGG 4 cut(s) 197, 198, 410, 411
SduI GDGCHC 1 cut(s) 316
SetI ASST 7 cut(s) 26, 100, 108, 189, 195, 612, 636
SfaNI GCATC 3 cut(s) 79, 718, 730
Sfr274I CTCGAG 2 cut(s) 535, 638
SlaI CTCGAG 2 cut(s) 535, 638
SmaI CCCGGG 2 cut(s) 198, 411
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 3 cut(s) 535, 638, 706
SmoI CTYRAG 3 cut(s) 535, 638, 706
Sse9I AATT 3 cut(s) 433, 464, 689
SsiI CCGC 2 cut(s) 35, 322
SspMI CTAG 1 cut(s) 543
StyD4I CCNGG 4 cut(s) 195, 196, 408, 409
TaaI ACNGT 3 cut(s) 29, 130, 254
TaiI ACGT 1 cut(s) 189
TaqI TCGA 3 cut(s) 536, 627, 639
TasI AATT 3 cut(s) 433, 464, 689
TatI WGTACW 1 cut(s) 702
TauI GCSGC 1 cut(s) 38
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TseFI GTSAC 3 cut(s) 241, 284, 362
TseI GCWGC 1 cut(s) 716
Tsp45I GTSAC 3 cut(s) 241, 284, 362
TspDTI ATGAA 3 cut(s) 64, 176, 573
TspGWI ACGGA 2 cut(s) 272, 660
TspMI CCCGGG 2 cut(s) 196, 409
Tth111I GACNNNGTC 1 cut(s) 386
XapI RAATTY 1 cut(s) 433
XceI RCATGY 2 cut(s) 475, 655
XhoI CTCGAG 2 cut(s) 535, 638
XmaI CCCGGG 2 cut(s) 196, 409
XspI CTAG 1 cut(s) 543
ZrmI AGTACT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.