MD07G1202200.v1.1

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000911
Physical Location & Seq
Reverse (-)
28023495 .. 28025625
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1202200.v1.1.491

Sequence Viewer

Length: 876 bp
ATGGGTAAGTCACGGACAGGTTTTAAAAAAAGAAAAAGAGATAAGGTTCCTCTCATCTTGGGTATTTGGGGAGGCAAAGGTCAAGGTAAATCTTTCCAGTGTAAGATTGTCTTTGCCAAGATGGGAATCAGCCCTATCATGATGAGTGCCGGAGAATTGGAAAGTGGAAACGCAGGAGAACCCGCGAAGGTGATCAGGCAAAGGTACCGTGAAGCGGCCGACATTATCAAGAAGGGTAAAATGTGTGCACTCTTCATCAACGATCTTGATGCAGGAGCTGGTCGGCTTGGTGGAACCACCCAATACACGGTCAACAACCAACTGGTGAATGCCACCCTCATGAACATTGCTGATAACCCGACAAACGTCCAGCTCCCAGGTATGTACAACAAGGAGGAGAACCCCCGTGTCCCAATCATTGTCACCGGTAACGATTTCTCAACACTGTACGCTCCTCTCATCCGTGACGGCCGTATGGAGAAGTTCTACTGGGCTCCCACCCGCGAAGATTGCATTGGAGTCTGCATTGGGATCTTCAGGAGTGACAATGTTGCTAAGGAAGACATTGTCAAGCTTGTTGACACCTTCCCGGGACAATCTATTGATTTCTTTGGTGCCTTGAGGGCTAGGGTTTACGACGATGAAGTGAGGAAGTGGATTTCGGGTGTTGGCGTGGAGGGCATTGGGAAGAAGCTTGTGAACTCAAAGGAAGGACCCCCAACTTTCGAGCAGCCGAATATGACAATCGAAAAGCTCCTCGAGTACGGAAACATGCTTGTCCAAGAGCAAGAGAATGTGAAGAGAGTTCAATTGGCTGATAAGTACTTGAGCGAGGCTGCTCTTGGTGATGCCAATTCAGATGCTATGGACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

292

Amino Acids

32.18

Weight (kDa)

8.88

Isoelectric Point (pI)

27.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 22 - 165 2e-16 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 168 - 263 1.7e-40 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 204
AccB1I GGYRCC 2 cut(s) 204, 614
AccII CGCG 2 cut(s) 185, 504
AciI CCGC 3 cut(s) 183, 215, 502
AclWI GGATC 1 cut(s) 539
AcoI YGGCCR 2 cut(s) 216, 469
AcuI CTGAAG 1 cut(s) 520
AfaI GTAC 5 cut(s) 206, 386, 449, 764, 824
AfiI CCNNNNNNNGG 2 cut(s) 214, 307
AgeI ACCGGT 1 cut(s) 425
AgsI TTSAA 1 cut(s) 809
AjnI CCWGG 1 cut(s) 376
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
AluBI AGCT 5 cut(s) 278, 373, 574, 694, 754
AluI AGCT 5 cut(s) 278, 373, 574, 694, 754
Alw21I GWGCWC 1 cut(s) 250
Alw44I GTGCAC 1 cut(s) 246
AlwI GGATC 1 cut(s) 539
AlwNI CAGNNNCTG 1 cut(s) 278
Ama87I CYCGRG 2 cut(s) 589, 758
AoxI GGCC 2 cut(s) 216, 469
ApaLI GTGCAC 1 cut(s) 246
ApeKI GCWGC 2 cut(s) 730, 836
AsiGI ACCGGT 1 cut(s) 425
Asp718I GGTACC 1 cut(s) 204
AspS9I GGNCC 1 cut(s) 713
AsuC2I CCSGG 2 cut(s) 590, 591
AsuHPI GGTGA 4 cut(s) 202, 337, 415, 857
AvaI CYCGRG 2 cut(s) 589, 758
AvaII GGWCC 1 cut(s) 713
BaeGI GKGCMC 1 cut(s) 250
BanI GGYRCC 2 cut(s) 204, 614
BanII GRGCYC 1 cut(s) 496
BbsI GAAGAC 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 2 cut(s) 742, 823
BccI CCATC 1 cut(s) 115
BceAI ACGGC 2 cut(s) 456, 484
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 1 cut(s) 378
BclI TGATCA 1 cut(s) 192
BcnI CCSGG 2 cut(s) 590, 591
BfaI CTAG 1 cut(s) 627
BglI GCCNNNNNGGC 1 cut(s) 623
BisI GCNGC 3 cut(s) 216, 731, 837
BlsI GCNGC 3 cut(s) 217, 732, 838
BmcAI AGTACT 1 cut(s) 824
Bme1390I CCNGG 3 cut(s) 378, 590, 591
Bme18I GGWCC 1 cut(s) 713
BmeT110I CYCGRG 2 cut(s) 589, 758
BmgT120I GGNCC 1 cut(s) 713
BmiI GGNNCC 6 cut(s) 48, 206, 295, 495, 616, 715
BmrFI CCNGG 3 cut(s) 378, 590, 591
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 3 cut(s) 259, 838, 850
BmuI ACTGGG 1 cut(s) 499
BoxI GACNNNNGTC 1 cut(s) 365
BpiI GAAGAC 1 cut(s) 567
Bpu10I CCTNAGC 1 cut(s) 555
BpuEI CTTGAG 2 cut(s) 640, 847
BpuMI CCSGG 2 cut(s) 590, 591
BsaBI GATNNNNATC 1 cut(s) 125
BsaJI CCNNGG 2 cut(s) 376, 589
BsaWI WCCGGW 1 cut(s) 425
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 307
Bse118I RCCGGY 1 cut(s) 425
Bse1I ACTGG 3 cut(s) 97, 327, 494
Bse3DI GCAATG 1 cut(s) 345
Bse8I GATNNNNATC 1 cut(s) 125
BseBI CCWGG 1 cut(s) 378
BseDI CCNNGG 2 cut(s) 376, 589
BseGI GGATG 1 cut(s) 459
BseJI GATNNNNATC 1 cut(s) 125
BseLI CCNNNNNNNGG 2 cut(s) 214, 307
BseMI GCAATG 1 cut(s) 345
BseNI ACTGG 3 cut(s) 97, 327, 494
BseRI GAGGAG 3 cut(s) 410, 444, 746
BseSI GKGCMC 1 cut(s) 250
BseX3I CGGCCG 2 cut(s) 216, 469
BseXI GCAGC 2 cut(s) 742, 823
Bsh1236I CGCG 2 cut(s) 185, 504
Bsh1285I CGRYCG 2 cut(s) 219, 472
BshFI GGCC 2 cut(s) 218, 471
BshNI GGYRCC 2 cut(s) 204, 614
BshTI ACCGGT 1 cut(s) 425
BsiEI CGRYCG 2 cut(s) 219, 472
BsiHKAI GWGCWC 1 cut(s) 250
BsiHKCI CYCGRG 2 cut(s) 589, 758
BsiSI CCGG 3 cut(s) 150, 426, 590
BslFI GGGAC 2 cut(s) 395, 606
BslI CCNNNNNNNGG 2 cut(s) 214, 307
BsmFI GGGAC 2 cut(s) 395, 606
BsmI GAATGC 1 cut(s) 334
BsnI GGCC 2 cut(s) 218, 471
BsoBI CYCGRG 2 cut(s) 589, 758
Bsp1286I GDGCHC 2 cut(s) 250, 496
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 3 cut(s) 192, 262, 531
BspACI CCGC 3 cut(s) 183, 215, 502
BspANI GGCC 2 cut(s) 218, 471
BspFNI CGCG 2 cut(s) 185, 504
BspHI TCATGA 2 cut(s) 138, 339
BspLI GGNNCC 6 cut(s) 48, 206, 295, 495, 616, 715
BspPI GGATC 1 cut(s) 539
BspT107I GGYRCC 2 cut(s) 204, 614
BsrDI GCAATG 1 cut(s) 345
BsrFI RCCGGY 1 cut(s) 425
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 3 cut(s) 97, 327, 494
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 2 cut(s) 376, 589
BssMI GATC 3 cut(s) 192, 262, 531
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 3 cut(s) 209, 310, 447
Bst6I CTCTTC 2 cut(s) 257, 794
BstAUI TGTACA 1 cut(s) 384
BstDEI CTNAG 1 cut(s) 555
BstF5I GGATG 1 cut(s) 459
BstFNI CGCG 2 cut(s) 185, 504
BstKTI GATC 3 cut(s) 195, 265, 534
BstMBI GATC 3 cut(s) 192, 262, 531
BstMCI CGRYCG 2 cut(s) 219, 472
BstMWI GCNNNNNNNGC 3 cut(s) 510, 623, 678
BstNI CCWGG 1 cut(s) 378
BstNSI RCATGY 1 cut(s) 775
BstPAI GACNNNNGTC 1 cut(s) 365
BstSCI CCNGG 3 cut(s) 376, 588, 589
BstSLI GKGCMC 1 cut(s) 250
BstUI CGCG 2 cut(s) 185, 504
BstV1I GCAGC 2 cut(s) 742, 823
BstV2I GAAGAC 1 cut(s) 567
BstX2I RGATCY 1 cut(s) 531
BstYI RGATCY 1 cut(s) 531
BstZI CGGCCG 2 cut(s) 216, 469
BsuRI GGCC 2 cut(s) 218, 471
BtsCI GGATG 1 cut(s) 459
BtsIMutI CAGTG 2 cut(s) 104, 443
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 2 cut(s) 138, 339
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 1 cut(s) 713
Cfr9I CCCGGG 1 cut(s) 589
Csp6I GTAC 5 cut(s) 205, 385, 448, 763, 823
CspAI ACCGGT 1 cut(s) 425
CviAII CATG 3 cut(s) 139, 340, 772
CviQI GTAC 5 cut(s) 205, 385, 448, 763, 823
DdeI CTNAG 1 cut(s) 555
DpnI GATC 3 cut(s) 194, 264, 533
DpnII GATC 3 cut(s) 192, 262, 531
DraI TTTAAA 1 cut(s) 25
EaeI YGGCCR 2 cut(s) 216, 469
EagI CGGCCG 2 cut(s) 216, 469
Eam1104I CTCTTC 2 cut(s) 257, 794
EarI CTCTTC 2 cut(s) 257, 794
EclXI CGGCCG 2 cut(s) 216, 469
Eco24I GRGCYC 1 cut(s) 496
Eco47I GGWCC 1 cut(s) 713
Eco52I CGGCCG 2 cut(s) 216, 469
Eco57I CTGAAG 1 cut(s) 520
Eco88I CYCGRG 2 cut(s) 589, 758
EcoO109I RGGNCCY 1 cut(s) 713
EcoRII CCWGG 1 cut(s) 376
EcoT38I GRGCYC 1 cut(s) 496
FaeI CATG 3 cut(s) 142, 343, 775
FaiI YATR 7 cut(s) 140, 341, 383, 476, 740, 773, 866
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FaqI GGGAC 2 cut(s) 395, 606
FatI CATG 3 cut(s) 138, 339, 771
FauI CCCGC 2 cut(s) 190, 509
FbaI TGATCA 1 cut(s) 192
Fnu4HI GCNGC 3 cut(s) 216, 731, 837
FokI GGATG 1 cut(s) 446
FriOI GRGCYC 1 cut(s) 496
Fsp4HI GCNGC 3 cut(s) 216, 731, 837
FspBI CTAG 1 cut(s) 627
GluI GCNGC 3 cut(s) 216, 731, 837
HaeIII GGCC 2 cut(s) 218, 471
HapII CCGG 3 cut(s) 150, 426, 590
Hin1II CATG 3 cut(s) 142, 343, 775
HincII GTYRAC 2 cut(s) 313, 580
HindII GTYRAC 2 cut(s) 313, 580
HindIII AAGCTT 2 cut(s) 572, 692
HinfI GANTC 2 cut(s) 126, 519
HpaII CCGG 3 cut(s) 150, 426, 590
HphI GGTGA 4 cut(s) 202, 337, 415, 857
Hpy166II GTNNAC 5 cut(s) 248, 313, 580, 634, 700
Hpy188I TCNGA 1 cut(s) 859
Hpy188III TCNNGA 5 cut(s) 139, 229, 266, 340, 538
Hpy8I GTNNAC 5 cut(s) 248, 313, 580, 634, 700
Hpy99I CGWCG 1 cut(s) 641
HpyAV CCTTC 4 cut(s) 181, 226, 595, 704
HpyCH4III ACNGT 3 cut(s) 209, 310, 447
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 4 cut(s) 248, 272, 513, 525
HpyF10VI GCNNNNNNNGC 3 cut(s) 510, 623, 678
HpyF3I CTNAG 1 cut(s) 555
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 3 cut(s) 142, 343, 775
KpnI GGTACC 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 3 cut(s) 192, 262, 531
LmnI GCTCC 5 cut(s) 275, 378, 457, 499, 759
Lsp1109I GCAGC 2 cut(s) 742, 823
LweI GCATC 3 cut(s) 259, 838, 850
MaeI CTAG 1 cut(s) 627
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 5 cut(s) 9, 421, 428, 464, 542
MalI GATC 3 cut(s) 194, 264, 533
MboI GATC 3 cut(s) 192, 262, 531
MboII GAAGA 6 cut(s) 244, 518, 526, 572, 700, 811
MfeI CAATTG 1 cut(s) 809
MflI RGATCY 1 cut(s) 531
MhlI GDGCHC 2 cut(s) 250, 496
MluCI AATT 3 cut(s) 155, 809, 853
MlyI GAGTC 1 cut(s) 528
MseI TTAA 1 cut(s) 24
MslI CAYNNNNRTG 1 cut(s) 338
MspI CCGG 3 cut(s) 150, 426, 590
MspR9I CCNGG 3 cut(s) 378, 590, 591
MunI CAATTG 1 cut(s) 809
Mva1269I GAATGC 1 cut(s) 334
MvaI CCWGG 1 cut(s) 378
MvnI CGCG 2 cut(s) 185, 504
MwoI GCNNNNNNNGC 3 cut(s) 510, 623, 678
NciI CCSGG 2 cut(s) 590, 591
NdeII GATC 3 cut(s) 192, 262, 531
NlaIII CATG 3 cut(s) 142, 343, 775
NlaIV GGNNCC 6 cut(s) 48, 206, 295, 495, 616, 715
NmuCI GTSAC 4 cut(s) 9, 421, 464, 542
NspI RCATGY 1 cut(s) 775
PaeR7I CTCGAG 1 cut(s) 758
PagI TCATGA 2 cut(s) 138, 339
PctI GAATGC 1 cut(s) 334
PfeI GAWTC 1 cut(s) 126
PflFI GACNNNGTC 1 cut(s) 566
PinAI ACCGGT 1 cut(s) 425
PkrI GCNGC 3 cut(s) 217, 732, 838
PleI GAGTC 1 cut(s) 527
PpsI GAGTC 1 cut(s) 527
PpuMI RGGWCCY 1 cut(s) 713
PshAI GACNNNNGTC 1 cut(s) 365
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 6 cut(s) 48, 206, 295, 495, 616, 715
PspPI GGNCC 1 cut(s) 713
PspPPI RGGWCCY 1 cut(s) 713
PspXI VCTCGAGB 1 cut(s) 758
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 531
PsyI GACNNNGTC 1 cut(s) 566
RsaI GTAC 5 cut(s) 206, 386, 449, 764, 824
RsaNI GTAC 5 cut(s) 205, 385, 448, 763, 823
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 1 cut(s) 24
SatI GCNGC 3 cut(s) 216, 731, 837
Sau3AI GATC 3 cut(s) 192, 262, 531
Sau96I GGNCC 1 cut(s) 713
ScaI AGTACT 1 cut(s) 824
SchI GAGTC 1 cut(s) 528
ScrFI CCNGG 3 cut(s) 378, 590, 591
SduI GDGCHC 2 cut(s) 250, 496
SfaNI GCATC 3 cut(s) 259, 838, 850
Sfr274I CTCGAG 1 cut(s) 758
SinI GGWCC 1 cut(s) 713
SlaI CTCGAG 1 cut(s) 758
SmaI CCCGGG 1 cut(s) 591
SmiMI CAYNNNNRTG 1 cut(s) 338
SmlI CTYRAG 3 cut(s) 619, 758, 826
SmoI CTYRAG 3 cut(s) 619, 758, 826
Sse9I AATT 3 cut(s) 155, 809, 853
SsiI CCGC 3 cut(s) 183, 215, 502
SspMI CTAG 1 cut(s) 627
StyD4I CCNGG 3 cut(s) 376, 588, 589
TaaI ACNGT 3 cut(s) 209, 310, 447
TaiI ACGT 1 cut(s) 369
TaqI TCGA 3 cut(s) 726, 747, 759
TasI AATT 3 cut(s) 155, 809, 853
TatI WGTACW 2 cut(s) 384, 822
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 1 cut(s) 24
Tru9I TTAA 1 cut(s) 24
TscAI CASTG 2 cut(s) 104, 450
TseFI GTSAC 4 cut(s) 9, 421, 464, 542
TseI GCWGC 2 cut(s) 730, 836
Tsp45I GTSAC 4 cut(s) 9, 421, 464, 542
TspDTI ATGAA 3 cut(s) 244, 356, 657
TspGWI ACGGA 3 cut(s) 28, 452, 780
TspMI CCCGGG 1 cut(s) 589
TspRI CASTG 2 cut(s) 104, 450
Tth111I GACNNNGTC 1 cut(s) 566
VneI GTGCAC 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 713
XceI RCATGY 1 cut(s) 775
XhoI CTCGAG 1 cut(s) 758
XmaI CCCGGG 1 cut(s) 589
XspI CTAG 1 cut(s) 627
ZrmI AGTACT 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.