Prupe.6G070800_v2.0.a1

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000911
Physical Location & Seq
Forward (+)
4849054 .. 4852183
3130 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G070800.1

Sequence Viewer

Length: 1428 bp
ATGGCTGCCACAGTCTCAAGTGTTGGAACTGTTAACAGAACACCGATGAGCTTGAACAGCTCTGGTTCTGGAAGCTCAATCCCAAGCTCAGCTTTCTTGGGCAGCAGTTTGAAGAAAGTCAGCTCTAGAGTCCCCTACCAGAAGATTGTATCTGCAGGTAAATTAAAAATTACTGCGGAAATTGGTGATGAGGAGAAGCAGACCAAAGAGGACAGGTGGAGGGGCCTTGCCTATGATATTTCTGATGACCAACAAGACATAACAAGAGGAAAGGGATTGGTTGATTCTATCTTCCAAGCTCCCCAGGGGTCTGGGACTCACTATGCTGTCATGAGCTCGTATGAATACATTAGCACGGGACTTCGAGAATACAACTATGACAACACCATGGATGGGTTTTATATTGCTCCAGCTTTCATGGACAAGCTTGTGGTTCATATCACCAAAAACTTCATGAGCCTGCCTAACATTAAGGTTCCTCTCATTCTTGGTATATGGGGAGGCAAAGGTCAGGGTAAATCGTTCCAATGTGAGCTTGTGTTTGCCAAGATGGGAATTACCCCTATCATGATGAGTGCTGGAGAATTAGAAAGCGGGAATGCAGGAGAGCCTGCAAAGTTGATCAGACAAAGGTACCGCGAGGCTGCAGACATCATCAGGAAAGGAAAAATGTGTGCCCTCTTCATCAATGATCTTGATGCAGGTGCTGGTAGGATGGGTGGAACTACACAGTACACTGTGAACAACCAGATGGTGAATGCTACTCTCATGAACATTGCTGATAACCCAACAAATGTGCAGCTTCCTGGTATGTATAACAAGGAGGACAATCCCAGAGTCCCCATTATAGTCACCGGTAACGATTTCTCCACATTGTATGCTCCTCTCATTCGTGACGGTCGTATGGAGAAATTTTACTGGGCTCCCACTAGGGAAGACCGGATTGGGGTTTGCACGGGAATCTTCAAGCCTGACAATGTTAGTGCGGATGATGTTGTTAAGATTGTTGACACATTCCCTGGTCAATCTATTGACTTCTTTGGTGCTCTAAGGGCTCGAGTTTACGATGATGAAGTGAGGAAGTGGGTATCAGGTGTTGGGGTAGATGGCATTGGGAAGAAGCTTGTGAACTCAAAAGAAGGTCCCCCAACTTTTGAACAGCCTAAAATGACAGTGGAGAAGCTCCTTCACTATGGTCAAATGCTGGTTCAGGAGCAGGACAATGTGAAGAGAGTTCAACTGGCTGACAAGTACTTGAGTGATGCAGCCCTTGGAGATGCAAACCAGGACGCCATTAAGAGAGGAGAATTCTATGGCAAGGCAGCCCAACAAATTAAAGTACCTGTCCCTGAAGGCTGTACTGATCCATCAGCTGCAAACTTTGACCCAACAGCTAGGAGTGACAATGGTAGTTGCCAATATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

476

Amino Acids

51.97

Weight (kDa)

6.1

Isoelectric Point (pI)

35.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 692
Acc36I ACCTGC 2 cut(s) 146, 692
Acc65I GGTACC 1 cut(s) 633
AccB1I GGYRCC 1 cut(s) 633
AccII CGCG 1 cut(s) 639
AciI CCGC 4 cut(s) 176, 594, 637, 986
AclWI GGATC 1 cut(s) 1358
AcsI RAATTY 2 cut(s) 911, 1307
AcuI CTGAAG 1 cut(s) 1371
AcyI GRCGYC 1 cut(s) 1290
AfaI GTAC 5 cut(s) 635, 734, 1253, 1341, 1360
AfiI CCNNNNNNNGG 2 cut(s) 393, 946
AgeI ACCGGT 1 cut(s) 854
AgsI TTSAA 5 cut(s) 55, 112, 967, 1157, 1238
AjnI CCWGG 4 cut(s) 303, 805, 1018, 1284
AjuI GAANNNNNNNTTGG 2 cut(s) 927, 959
Alw21I GWGCWC 2 cut(s) 338, 1048
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 1 cut(s) 1358
AlwNI CAGNNNCTG 1 cut(s) 707
Ama87I CYCGRG 1 cut(s) 1056
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 7 cut(s) 5, 102, 644, 799, 1265, 1322, 1373
ApoI RAATTY 2 cut(s) 911, 1307
AsiGI ACCGGT 1 cut(s) 854
Asp718I GGTACC 1 cut(s) 633
AspS9I GGNCC 2 cut(s) 223, 1142
AsuHPI GGTGA 4 cut(s) 197, 433, 766, 844
AvaI CYCGRG 1 cut(s) 1056
AvaII GGWCC 1 cut(s) 1142
BaeGI GKGCMC 1 cut(s) 679
BaeI ACNNNNGTAYC 2 cut(s) 617, 650
BanI GGYRCC 1 cut(s) 633
BanII GRGCYC 3 cut(s) 338, 925, 1057
BbsI GAAGAC 1 cut(s) 942
Bbv12I GWGCWC 2 cut(s) 338, 1048
BbvI GCAGC 6 cut(s) 114, 631, 811, 1277, 1334, 1360
BccI CCATC 6 cut(s) 386, 544, 709, 745, 1100, 1375
BciT130I CCWGG 4 cut(s) 305, 807, 1020, 1286
BclI TGATCA 1 cut(s) 621
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 3 cut(s) 126, 930, 1395
BfmI CTRYAG 2 cut(s) 153, 645
BfuAI ACCTGC 2 cut(s) 146, 692
BisI GCNGC 7 cut(s) 6, 103, 645, 800, 1266, 1323, 1374
BlpI GCTNAGC 1 cut(s) 88
BlsI GCNGC 7 cut(s) 7, 104, 646, 801, 1267, 1324, 1375
BmcAI AGTACT 1 cut(s) 1253
Bme1390I CCNGG 4 cut(s) 305, 807, 1020, 1286
Bme18I GGWCC 1 cut(s) 1142
BmeT110I CYCGRG 1 cut(s) 1056
BmgT120I GGNCC 2 cut(s) 223, 1142
BmiI GGNNCC 5 cut(s) 224, 477, 635, 924, 1144
BmrFI CCNGG 4 cut(s) 305, 807, 1020, 1286
BmrI ACTGGG 1 cut(s) 928
BmsI GCATC 3 cut(s) 688, 1252, 1267
BmuI ACTGGG 1 cut(s) 928
BpiI GAAGAC 1 cut(s) 942
BpmI CTGGAG 2 cut(s) 393, 600
Bpu1102I GCTNAGC 1 cut(s) 88
BpuEI CTTGAG 1 cut(s) 1276
BsaHI GRCGYC 1 cut(s) 1290
BsaJI CCNNGG 5 cut(s) 303, 304, 387, 1018, 1270
BsaWI WCCGGW 2 cut(s) 854, 939
BsaXI ACNNNNNCTCC 2 cut(s) 851, 881
Bsc4I CCNNNNNNNGG 2 cut(s) 393, 946
Bse118I RCCGGY 1 cut(s) 854
Bse1I ACTGG 2 cut(s) 923, 1245
Bse3DI GCAATG 1 cut(s) 774
BseBI CCWGG 4 cut(s) 305, 807, 1020, 1286
BseDI CCNNGG 5 cut(s) 303, 304, 387, 1018, 1270
BseGI GGATG 3 cut(s) 397, 720, 994
BseLI CCNNNNNNNGG 2 cut(s) 393, 946
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 1 cut(s) 102
BseNI ACTGG 2 cut(s) 923, 1245
BseRI GAGGAG 3 cut(s) 206, 873, 1317
BseSI GKGCMC 1 cut(s) 679
BseXI GCAGC 6 cut(s) 114, 631, 811, 1277, 1334, 1360
BsgI GTGCAG 1 cut(s) 818
Bsh1236I CGCG 1 cut(s) 639
Bsh1285I CGRYCG 1 cut(s) 901
BshFI GGCC 1 cut(s) 225
BshNI GGYRCC 1 cut(s) 633
BshTI ACCGGT 1 cut(s) 854
BsiEI CGRYCG 1 cut(s) 901
BsiHKAI GWGCWC 2 cut(s) 338, 1048
BsiHKCI CYCGRG 1 cut(s) 1056
BsiSI CCGG 2 cut(s) 855, 940
BslFI GGGAC 6 cut(s) 116, 328, 372, 824, 1128, 1331
BslI CCNNNNNNNGG 2 cut(s) 393, 946
BsmAI GTCTC 1 cut(s) 19
BsmFI GGGAC 6 cut(s) 116, 328, 372, 824, 1128, 1331
BsmI GAATGC 2 cut(s) 604, 763
BsnI GGCC 1 cut(s) 225
BsoBI CYCGRG 1 cut(s) 1056
Bsp1286I GDGCHC 5 cut(s) 338, 679, 925, 1048, 1057
Bsp143I GATC 3 cut(s) 621, 691, 1363
Bsp1720I GCTNAGC 1 cut(s) 88
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 4 cut(s) 176, 594, 637, 986
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 1 cut(s) 101
BspFNI CGCG 1 cut(s) 639
BspHI TCATGA 4 cut(s) 330, 453, 567, 768
BspLI GGNNCC 5 cut(s) 224, 477, 635, 924, 1144
BspMAI CTGCAG 2 cut(s) 157, 649
BspMI ACCTGC 2 cut(s) 146, 692
BspPI GGATC 1 cut(s) 1358
BspT107I GGYRCC 1 cut(s) 633
BsrDI GCAATG 1 cut(s) 774
BsrFI RCCGGY 1 cut(s) 854
BsrI ACTGG 2 cut(s) 923, 1245
BssAI RCCGGY 1 cut(s) 854
BssECI CCNNGG 5 cut(s) 303, 304, 387, 1018, 1270
BssMI GATC 3 cut(s) 621, 691, 1363
BssNI GRCGYC 1 cut(s) 1290
BssT1I CCWWGG 2 cut(s) 387, 1270
Bst2UI CCWGG 4 cut(s) 305, 807, 1020, 1286
Bst4CI ACNGT 6 cut(s) 13, 31, 732, 739, 899, 1174
Bst6I CTCTTC 2 cut(s) 686, 1223
BstACI GRCGYC 1 cut(s) 1290
BstC8I GCNNGC 2 cut(s) 461, 612
BstDEI CTNAG 2 cut(s) 88, 1049
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 3 cut(s) 397, 720, 994
BstFNI CGCG 1 cut(s) 639
BstKTI GATC 3 cut(s) 624, 694, 1366
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 3 cut(s) 621, 691, 1363
BstMCI CGRYCG 1 cut(s) 901
BstMWI GCNNNNNNNGC 2 cut(s) 57, 1052
BstNI CCWGG 4 cut(s) 305, 807, 1020, 1286
BstSCI CCNGG 4 cut(s) 303, 805, 1018, 1284
BstSFI CTRYAG 2 cut(s) 153, 645
BstSLI GKGCMC 1 cut(s) 679
BstUI CGCG 1 cut(s) 639
BstV1I GCAGC 6 cut(s) 114, 631, 811, 1277, 1334, 1360
BstV2I GAAGAC 1 cut(s) 942
BstXI CCANNNNNNTGG 1 cut(s) 311
BsuRI GGCC 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 3 cut(s) 397, 720, 994
BtsIMutI CAGTG 2 cut(s) 735, 1179
BveI ACCTGC 2 cut(s) 146, 692
Cac8I GCNNGC 2 cut(s) 461, 612
CaiI CAGNNNCTG 1 cut(s) 707
CciI TCATGA 4 cut(s) 330, 453, 567, 768
Cfr10I RCCGGY 1 cut(s) 854
Cfr13I GGNCC 2 cut(s) 223, 1142
CseI GACGC 1 cut(s) 1298
Csp6I GTAC 5 cut(s) 634, 733, 1252, 1340, 1359
CspAI ACCGGT 1 cut(s) 854
CviAII CATG 6 cut(s) 331, 388, 418, 454, 568, 769
CviQI GTAC 5 cut(s) 634, 733, 1252, 1340, 1359
DdeI CTNAG 2 cut(s) 88, 1049
DpnI GATC 3 cut(s) 623, 693, 1365
DpnII GATC 3 cut(s) 621, 691, 1363
Eam1104I CTCTTC 2 cut(s) 686, 1223
EarI CTCTTC 2 cut(s) 686, 1223
Ecl136II GAGCTC 1 cut(s) 336
Eco130I CCWWGG 2 cut(s) 387, 1270
Eco24I GRGCYC 3 cut(s) 338, 925, 1057
Eco47I GGWCC 1 cut(s) 1142
Eco53kI GAGCTC 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 1371
Eco88I CYCGRG 1 cut(s) 1056
EcoICRI GAGCTC 1 cut(s) 336
EcoO109I RGGNCCY 2 cut(s) 223, 1142
EcoRI GAATTC 1 cut(s) 1307
EcoRII CCWGG 4 cut(s) 303, 805, 1018, 1284
EcoT14I CCWWGG 2 cut(s) 387, 1270
EcoT38I GRGCYC 3 cut(s) 338, 925, 1057
ErhI CCWWGG 2 cut(s) 387, 1270
FaeI CATG 6 cut(s) 334, 391, 421, 457, 571, 772
FalI AAGNNNNNCTT 2 cut(s) 76, 108
FaqI GGGAC 6 cut(s) 116, 328, 372, 824, 1128, 1331
FatI CATG 6 cut(s) 330, 387, 417, 453, 567, 768
FauI CCCGC 1 cut(s) 587
FbaI TGATCA 1 cut(s) 621
Fnu4HI GCNGC 7 cut(s) 6, 103, 645, 800, 1266, 1323, 1374
FokI GGATG 3 cut(s) 404, 727, 1001
FriOI GRGCYC 3 cut(s) 338, 925, 1057
Fsp4HI GCNGC 7 cut(s) 6, 103, 645, 800, 1266, 1323, 1374
FspBI CTAG 3 cut(s) 126, 930, 1395
GluI GCNGC 7 cut(s) 6, 103, 645, 800, 1266, 1323, 1374
GsuI CTGGAG 2 cut(s) 393, 600
HaeIII GGCC 1 cut(s) 225
HapII CCGG 2 cut(s) 855, 940
HgaI GACGC 1 cut(s) 1298
Hin1I GRCGYC 1 cut(s) 1290
Hin1II CATG 6 cut(s) 334, 391, 421, 457, 571, 772
HincII GTYRAC 2 cut(s) 34, 1009
HindII GTYRAC 2 cut(s) 34, 1009
HindIII AAGCTT 2 cut(s) 425, 1121
HinfI GANTC 5 cut(s) 129, 284, 316, 837, 960
HpaI GTTAAC 1 cut(s) 34
HpaII CCGG 2 cut(s) 855, 940
HphI GGTGA 4 cut(s) 197, 433, 766, 844
Hpy166II GTNNAC 6 cut(s) 34, 735, 742, 1009, 1063, 1129
Hpy188I TCNGA 2 cut(s) 244, 626
Hpy8I GTNNAC 6 cut(s) 34, 735, 742, 1009, 1063, 1129
HpyAV CCTTC 3 cut(s) 1133, 1196, 1346
HpyCH4III ACNGT 6 cut(s) 13, 31, 732, 739, 899, 1174
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 1052
HpyF3I CTNAG 2 cut(s) 88, 1049
Hsp92I GRCGYC 1 cut(s) 1290
Hsp92II CATG 6 cut(s) 334, 391, 421, 457, 571, 772
KpnI GGTACC 1 cut(s) 637
Ksp22I TGATCA 1 cut(s) 621
KspAI GTTAAC 1 cut(s) 34
Kzo9I GATC 3 cut(s) 621, 691, 1363
LmnI GCTCC 6 cut(s) 304, 412, 886, 928, 1188, 1213
Lsp1109I GCAGC 6 cut(s) 114, 631, 811, 1277, 1334, 1360
LweI GCATC 3 cut(s) 688, 1252, 1267
MaeI CTAG 3 cut(s) 126, 930, 1395
MaeIII GTNAC 4 cut(s) 850, 857, 893, 1400
MalI GATC 3 cut(s) 623, 693, 1365
MboI GATC 3 cut(s) 621, 691, 1363
MboII GAAGA 8 cut(s) 124, 154, 283, 673, 947, 955, 1129, 1240
MhlI GDGCHC 5 cut(s) 338, 679, 925, 1048, 1057
MluCI AATT 8 cut(s) 161, 168, 180, 555, 584, 911, 1307, 1332
MlyI GAGTC 3 cut(s) 138, 310, 846
MmeI TCCRAC 1 cut(s) 4
MseI TTAA 6 cut(s) 33, 164, 471, 999, 1296, 1335
MspA1I CMGCKG 1 cut(s) 1373
MspI CCGG 2 cut(s) 855, 940
MspR9I CCNGG 4 cut(s) 305, 807, 1020, 1286
Mva1269I GAATGC 2 cut(s) 604, 763
MvaI CCWGG 4 cut(s) 305, 807, 1020, 1286
MvnI CGCG 1 cut(s) 639
MwoI GCNNNNNNNGC 2 cut(s) 57, 1052
NcoI CCATGG 1 cut(s) 387
NdeII GATC 3 cut(s) 621, 691, 1363
NlaIII CATG 6 cut(s) 334, 391, 421, 457, 571, 772
NlaIV GGNNCC 5 cut(s) 224, 477, 635, 924, 1144
NmuCI GTSAC 3 cut(s) 850, 893, 1400
PaeR7I CTCGAG 1 cut(s) 1056
PagI TCATGA 4 cut(s) 330, 453, 567, 768
PaqCI CACCTGC 1 cut(s) 692
PasI CCCWGGG 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 898
PctI GAATGC 2 cut(s) 604, 763
PfeI GAWTC 2 cut(s) 284, 960
PinAI ACCGGT 1 cut(s) 854
PkrI GCNGC 7 cut(s) 7, 104, 646, 801, 1267, 1324, 1375
PleI GAGTC 3 cut(s) 137, 310, 845
PpsI GAGTC 3 cut(s) 137, 310, 845
PpuMI RGGWCCY 1 cut(s) 1142
Psp124BI GAGCTC 1 cut(s) 338
Psp5II RGGWCCY 1 cut(s) 1142
Psp6I CCWGG 4 cut(s) 303, 805, 1018, 1284
PspGI CCWGG 4 cut(s) 303, 805, 1018, 1284
PspN4I GGNNCC 5 cut(s) 224, 477, 635, 924, 1144
PspPI GGNCC 2 cut(s) 223, 1142
PspPPI RGGWCCY 1 cut(s) 1142
PspXI VCTCGAGB 1 cut(s) 1056
PstI CTGCAG 2 cut(s) 157, 649
PstNI CAGNNNCTG 1 cut(s) 707
PvuII CAGCTG 1 cut(s) 1373
RsaI GTAC 5 cut(s) 635, 734, 1253, 1341, 1360
RsaNI GTAC 5 cut(s) 634, 733, 1252, 1340, 1359
SacI GAGCTC 1 cut(s) 338
SaqAI TTAA 6 cut(s) 33, 164, 471, 999, 1296, 1335
SatI GCNGC 7 cut(s) 6, 103, 645, 800, 1266, 1323, 1374
Sau3AI GATC 3 cut(s) 621, 691, 1363
Sau96I GGNCC 2 cut(s) 223, 1142
ScaI AGTACT 1 cut(s) 1253
SchI GAGTC 3 cut(s) 138, 310, 846
ScrFI CCNGG 4 cut(s) 305, 807, 1020, 1286
SduI GDGCHC 5 cut(s) 338, 679, 925, 1048, 1057
SfaNI GCATC 3 cut(s) 688, 1252, 1267
SfcI CTRYAG 2 cut(s) 153, 645
Sfr274I CTCGAG 1 cut(s) 1056
SinI GGWCC 1 cut(s) 1142
SlaI CTCGAG 1 cut(s) 1056
SmlI CTYRAG 3 cut(s) 16, 1056, 1255
SmoI CTYRAG 3 cut(s) 16, 1056, 1255
Sse9I AATT 8 cut(s) 161, 168, 180, 555, 584, 911, 1307, 1332
SsiI CCGC 4 cut(s) 176, 594, 637, 986
SspMI CTAG 3 cut(s) 126, 930, 1395
SstI GAGCTC 1 cut(s) 338
StyD4I CCNGG 4 cut(s) 303, 805, 1018, 1284
StyI CCWWGG 2 cut(s) 387, 1270
TaaI ACNGT 6 cut(s) 13, 31, 732, 739, 899, 1174
TaqI TCGA 2 cut(s) 364, 1057
TasI AATT 8 cut(s) 161, 168, 180, 555, 584, 911, 1307, 1332
TatI WGTACW 3 cut(s) 732, 1251, 1358
TfiI GAWTC 2 cut(s) 284, 960
Tru1I TTAA 6 cut(s) 33, 164, 471, 999, 1296, 1335
Tru9I TTAA 6 cut(s) 33, 164, 471, 999, 1296, 1335
TscAI CASTG 2 cut(s) 742, 1179
TseFI GTSAC 3 cut(s) 850, 893, 1400
TseI GCWGC 7 cut(s) 5, 102, 644, 799, 1265, 1322, 1373
Tsp45I GTSAC 3 cut(s) 850, 893, 1400
TspDTI ATGAA 7 cut(s) 357, 406, 425, 442, 673, 785, 1086
TspRI CASTG 2 cut(s) 742, 1179
VpaK11BI GGWCC 1 cut(s) 1142
XapI RAATTY 2 cut(s) 911, 1307
XbaI TCTAGA 1 cut(s) 125
XhoI CTCGAG 1 cut(s) 1056
XspI CTAG 3 cut(s) 126, 930, 1395
ZrmI AGTACT 1 cut(s) 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.