Rorug03G0077800

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000911
Physical Location & Seq
Forward (+)
6084851 .. 6085468
618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0077800.1

Sequence Viewer

Length: 483 bp
ATGGCATTCCTAGCAGTTAGGACACTAATTGTTCTTATGCTTATTTCTACATTTGCTAATGCCGCAAACAAATATGACTATCTACAACTAGTGCAACAATGGCCTAAAACGTTCTGCCATAATAACCAAGCTTGCATTCAAGGTGCAGCCCTCCCGGAGCTGTTCTCGATACATGGTATGTGGCCATCTAATTTCTCCGGCCAGAACGACGCTTGTGTTGGAACTCGGTTCAGCATGAGAGAGATGCGTCGACACAATCATCTCGAAACTCAATTGCTGTCATCCTCATGGCCAAGCTACACTGGTCTGTCCAACCTGCACTTTTGGGAGTACGAGTATAACAAGCACGGCACTTGTTCAGAGAATAACCTCAAACAGACGGACTATTTCACCAGAGCCGACGCTTTGTGGAGGCGCTACAATGTTACCAATATGCTTTTAACATCGCGCCACCAAATCTCGCCAGGATCCTCCTATCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

160

Amino Acids

18.61

Weight (kDa)

8.98

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 25 - 156 2e-27 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 324
AccI GTMKAC 1 cut(s) 250
AccII CGCG 1 cut(s) 448
AciI CCGC 1 cut(s) 63
AclI AACGTT 1 cut(s) 110
AclWI GGATC 2 cut(s) 462, 475
AcoI YGGCCR 3 cut(s) 182, 199, 290
AfaI GTAC 1 cut(s) 332
AgsI TTSAA 1 cut(s) 140
AhlI ACTAGT 1 cut(s) 88
AjnI CCWGG 1 cut(s) 463
AjuI GAANNNNNNNTTGG 2 cut(s) 120, 152
AluBI AGCT 3 cut(s) 131, 160, 297
AluI AGCT 3 cut(s) 131, 160, 297
AlwI GGATC 2 cut(s) 462, 475
AoxI GGCC 4 cut(s) 101, 182, 199, 290
ApeKI GCWGC 1 cut(s) 146
AspLEI GCGC 2 cut(s) 417, 450
AsuC2I CCSGG 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 382
BalI TGGCCA 2 cut(s) 184, 292
BamHI GGATCC 1 cut(s) 467
BbvI GCAGC 1 cut(s) 158
BccI CCATC 1 cut(s) 193
BceAI ACGGC 1 cut(s) 364
BciT130I CCWGG 1 cut(s) 465
BcnI CCSGG 1 cut(s) 155
BcuI ACTAGT 1 cut(s) 88
BfaI CTAG 2 cut(s) 11, 89
BfoI RGCGCY 1 cut(s) 418
BfuAI ACCTGC 1 cut(s) 324
BisI GCNGC 2 cut(s) 63, 147
BlsI GCNGC 2 cut(s) 64, 148
Bme1390I CCNGG 2 cut(s) 155, 465
BmiI GGNNCC 1 cut(s) 469
BmrFI CCNGG 2 cut(s) 155, 465
BmsI GCATC 1 cut(s) 234
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpuMI CCSGG 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bse1I ACTGG 1 cut(s) 307
BseBI CCWGG 1 cut(s) 465
BseGI GGATG 1 cut(s) 281
BseNI ACTGG 1 cut(s) 307
BseXI GCAGC 1 cut(s) 158
BsgI GTGCAG 2 cut(s) 165, 302
Bsh1236I CGCG 1 cut(s) 448
BshFI GGCC 4 cut(s) 103, 184, 201, 292
BsiSI CCGG 2 cut(s) 155, 198
BsmI GAATGC 2 cut(s) 5, 135
BsnI GGCC 4 cut(s) 103, 184, 201, 292
Bsp143I GATC 1 cut(s) 467
BspACI CCGC 1 cut(s) 63
BspANI GGCC 4 cut(s) 103, 184, 201, 292
BspFNI CGCG 1 cut(s) 448
BspLI GGNNCC 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 324
BspPI GGATC 2 cut(s) 462, 475
BsrI ACTGG 1 cut(s) 307
BssMI GATC 1 cut(s) 467
Bst2UI CCWGG 1 cut(s) 465
BstC8I GCNNGC 1 cut(s) 133
BstF5I GGATG 1 cut(s) 281
BstFNI CGCG 1 cut(s) 448
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 2 cut(s) 417, 450
BstKTI GATC 1 cut(s) 470
BstMBI GATC 1 cut(s) 467
BstMWI GCNNNNNNNGC 3 cut(s) 11, 62, 100
BstNI CCWGG 1 cut(s) 465
BstSCI CCNGG 2 cut(s) 153, 463
BstUI CGCG 1 cut(s) 448
BstV1I GCAGC 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
BsuRI GGCC 4 cut(s) 103, 184, 201, 292
BtgZI GCGATG 1 cut(s) 429
BtsCI GGATG 1 cut(s) 281
BtsIMutI CAGTG 1 cut(s) 300
BveI ACCTGC 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 133
CfoI GCGC 2 cut(s) 417, 450
CseI GACGC 3 cut(s) 218, 236, 410
Csp6I GTAC 1 cut(s) 331
CviAII CATG 3 cut(s) 173, 235, 288
CviJI RGCY 9 cut(s) 103, 131, 149, 160, 184, 201, 292, 297, 398
CviKI_1 RGCY 9 cut(s) 103, 131, 149, 160, 184, 201, 292, 297, 398
CviQI GTAC 1 cut(s) 331
DpnI GATC 1 cut(s) 469
DpnII GATC 1 cut(s) 467
EaeI YGGCCR 3 cut(s) 182, 199, 290
EcoRII CCWGG 1 cut(s) 463
FaeI CATG 3 cut(s) 176, 238, 291
FaiI YATR 9 cut(s) 38, 75, 120, 174, 179, 236, 289, 339, 434
FatI CATG 3 cut(s) 172, 234, 287
FblI GTMKAC 1 cut(s) 250
Fnu4HI GCNGC 2 cut(s) 63, 147
FokI GGATG 1 cut(s) 268
Fsp4HI GCNGC 2 cut(s) 63, 147
FspBI CTAG 2 cut(s) 11, 89
GlaI GCGC 2 cut(s) 416, 449
GluI GCNGC 2 cut(s) 63, 147
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 4 cut(s) 103, 184, 201, 292
HapII CCGG 2 cut(s) 155, 198
HgaI GACGC 3 cut(s) 218, 236, 410
HhaI GCGC 2 cut(s) 417, 450
Hin1II CATG 3 cut(s) 176, 238, 291
Hin6I GCGC 2 cut(s) 415, 448
HinP1I GCGC 2 cut(s) 415, 448
HincII GTYRAC 1 cut(s) 251
HindII GTYRAC 1 cut(s) 251
HindIII AAGCTT 1 cut(s) 129
HpaII CCGG 2 cut(s) 155, 198
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 1 cut(s) 251
Hpy188I TCNGA 1 cut(s) 361
Hpy188III TCNNGA 2 cut(s) 166, 263
Hpy8I GTNNAC 1 cut(s) 251
Hpy99I CGWCG 3 cut(s) 212, 252, 404
HpyCH4IV ACGT 1 cut(s) 110
HpyCH4V TGCA 4 cut(s) 94, 135, 146, 319
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 62, 100
HpySE526I ACGT 1 cut(s) 110
Hsp92II CATG 3 cut(s) 176, 238, 291
HspAI GCGC 2 cut(s) 415, 448
Kzo9I GATC 1 cut(s) 467
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 8 cut(s) 168, 211, 215, 288, 329, 406, 450, 477
Lsp1109I GCAGC 1 cut(s) 158
LweI GCATC 1 cut(s) 234
MaeI CTAG 2 cut(s) 11, 89
MaeII ACGT 1 cut(s) 110
MaeIII GTNAC 1 cut(s) 424
MalI GATC 1 cut(s) 469
MboI GATC 1 cut(s) 467
MfeI CAATTG 1 cut(s) 272
MflI RGATCY 1 cut(s) 467
MlsI TGGCCA 2 cut(s) 184, 292
MluCI AATT 3 cut(s) 27, 190, 272
MluNI TGGCCA 2 cut(s) 184, 292
MmeI TCCRAC 2 cut(s) 199, 336
MnlI CCTC 5 cut(s) 161, 295, 380, 405, 481
Mox20I TGGCCA 2 cut(s) 184, 292
MscI TGGCCA 2 cut(s) 184, 292
MseI TTAA 2 cut(s) 440, 481
MslI CAYNNNNRTG 1 cut(s) 286
Msp20I TGGCCA 2 cut(s) 184, 292
MspI CCGG 2 cut(s) 155, 198
MspR9I CCNGG 2 cut(s) 155, 465
MunI CAATTG 1 cut(s) 272
Mva1269I GAATGC 2 cut(s) 5, 135
MvaI CCWGG 1 cut(s) 465
MvnI CGCG 1 cut(s) 448
MwoI GCNNNNNNNGC 3 cut(s) 11, 62, 100
NciI CCSGG 1 cut(s) 155
NdeII GATC 1 cut(s) 467
NlaIII CATG 3 cut(s) 176, 238, 291
NlaIV GGNNCC 1 cut(s) 469
PctI GAATGC 2 cut(s) 5, 135
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 2 cut(s) 64, 148
Psp1406I AACGTT 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 463
PspGI CCWGG 1 cut(s) 463
PspN4I GGNNCC 1 cut(s) 469
PsuI RGATCY 1 cut(s) 467
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
RseI CAYNNNNRTG 1 cut(s) 286
SalI GTCGAC 1 cut(s) 249
SaqAI TTAA 2 cut(s) 440, 481
SatI GCNGC 2 cut(s) 63, 147
Sau3AI GATC 1 cut(s) 467
ScrFI CCNGG 2 cut(s) 155, 465
SetI ASST 7 cut(s) 113, 133, 145, 162, 299, 318, 372
SfaNI GCATC 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 286
SpeI ACTAGT 1 cut(s) 88
Sse9I AATT 3 cut(s) 27, 190, 272
SsiI CCGC 1 cut(s) 63
SspMI CTAG 2 cut(s) 11, 89
StyD4I CCNGG 2 cut(s) 153, 463
TaiI ACGT 1 cut(s) 113
TaqI TCGA 3 cut(s) 167, 250, 264
TasI AATT 3 cut(s) 27, 190, 272
TauI GCSGC 1 cut(s) 65
Tru1I TTAA 2 cut(s) 440, 481
Tru9I TTAA 2 cut(s) 440, 481
TscAI CASTG 1 cut(s) 307
TseI GCWGC 1 cut(s) 146
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 250
XspI CTAG 2 cut(s) 11, 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.