Rorug01G0338800

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000911
Physical Location & Seq
Forward (+)
45676710 .. 45678238
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0338800.1

Sequence Viewer

Length: 1128 bp
ATGGCAACCAAAGCATGCCTATTTTTCATGCTTAGCTCCTTGTTTCTTCTTTGGAAGTTAAAAGTTAGCTCCTCGTTTCTTCTTCGGAATTTAAGCAATGCAGCATCATTGAAAGTTTGTAACTTGGAAGCCATATATCAATTTGGGGATTCTTTCTCCGACACCGGAAATGCGATACGTGAAAACAAAGCTCTAGAGGCTACTAAGCTCCCTTATGGCTTAAATCTTCCCAGGGAACCAAAAGGTCGATTTTCCGACGGGATGTTGGTTATAGATTTTGAAGCACAAGCAGCTGGGCTTCCATTTCTTAAGCCATATTTGGATCGGGAAGCGAAAGATTACCACAACGGAAAGAACTTTGCTGTTAGTTCTGCTAGCGTTTTGCCAAAAGGTGTTCTTAAAGAGAAATCAATATTCTTCCCAGAAACAATGACAAACATTACGCTACATAAGCAAGTGGATTGGATGGCCACTTACTTCAATGAAACCCCCTATTTTAGTAAAGACCGCGCAGAGAAGCTTAAGAGGTCACTCTTTTTCATCGGACCAATGGCACATGGTGATTATATGTATTCAATGGACTTGAGTCGTAAATCTTCAGAATGGGTAAAGAGCAATTTGGTTCCAGATATCGTCCAAACCATCAAGGAAGCTGCCACAAGGGTCATTGCTTTTGGTGCTGTGAAAGTGGTCATCCCAGGAATATACGCATTTGGTTGTTTTAAAGCTCCAGGTTATAATGTATCGCTATGCGACCAGGGCAAAAATAGTGTTGCAACCACTCACAACGATCTGCTTCGGAGAGCAATTGAGGAGTTAAAGAAACAACACCCTAATGTGACTATTGTATATGCAGATTATTACAGTGCATTCATGTCCTTCTTCCCCAAGGCACTTAGTCTAGGGTTTGAACCTGCGAACAAGCCATGCTGTTTCGGTACACACAAAAATGGTCCAGGACATGGTTTTAACCCGGATAACATGTGTGGAAAGCCTGGAGTACAAGCTTGTGCTACCCCTGCGAAACGAATCAACTGGGATGGTAGTCATATGACGCAACATGCATACAAGTTAATAGCAGATTTGATAATTCCTGACATCGCAGCCAAGCTTAAATGCAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

375

Amino Acids

41.67

Weight (kDa)

9.14

Isoelectric Point (pI)

30.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 363 1.1e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 738
Acc36I ACCTGC 1 cut(s) 922
AccB7I CCANNNNNTGG 1 cut(s) 962
AccII CGCG 1 cut(s) 510
AciI CCGC 1 cut(s) 508
AclWI GGATC 1 cut(s) 330
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 1 cut(s) 88
AcuI CTGAAG 1 cut(s) 582
AdeI CACNNNGTG 1 cut(s) 560
AfaI GTAC 2 cut(s) 940, 1002
AfiI CCNNNNNNNGG 1 cut(s) 962
AflII CTTAAG 2 cut(s) 308, 521
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 5 cut(s) 112, 281, 481, 576, 911
AjnI CCWGG 6 cut(s) 230, 697, 730, 756, 955, 994
AlwI GGATC 1 cut(s) 330
AoxI GGCC 1 cut(s) 468
ApeKI GCWGC 4 cut(s) 101, 290, 653, 1103
ApoI RAATTY 1 cut(s) 88
AspLEI GCGC 1 cut(s) 512
AspS9I GGNCC 2 cut(s) 545, 953
AsuC2I CCSGG 1 cut(s) 974
AsuHPI GGTGA 1 cut(s) 572
AsuNHI GCTAGC 1 cut(s) 374
AvaII GGWCC 2 cut(s) 545, 953
BalI TGGCCA 1 cut(s) 470
BbvI GCAGC 4 cut(s) 113, 302, 640, 1115
BccI CCATC 3 cut(s) 460, 650, 1034
BciT130I CCWGG 6 cut(s) 232, 699, 732, 758, 957, 996
BcnI CCSGG 1 cut(s) 974
BfaI CTAG 3 cut(s) 194, 375, 902
BfrI CTTAAG 2 cut(s) 308, 521
BfuAI ACCTGC 1 cut(s) 922
BisI GCNGC 4 cut(s) 102, 291, 654, 1104
BlpI GCTNAGC 1 cut(s) 32
BlsI GCNGC 4 cut(s) 103, 292, 655, 1105
Bme1390I CCNGG 7 cut(s) 232, 699, 732, 758, 957, 974, 996
Bme18I GGWCC 2 cut(s) 545, 953
BmgT120I GGNCC 2 cut(s) 545, 953
BmiI GGNNCC 2 cut(s) 237, 624
BmrFI CCNGG 7 cut(s) 232, 699, 732, 758, 957, 974, 996
BmrI ACTGGG 1 cut(s) 1045
BmsI GCATC 1 cut(s) 113
BmtI GCTAGC 1 cut(s) 378
BmuI ACTGGG 1 cut(s) 1045
BoxI GACNNNNGTC 1 cut(s) 585
BpmI CTGGAG 2 cut(s) 714, 1017
Bpu1102I GCTNAGC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 604
BpuMI CCSGG 1 cut(s) 974
BsaAI YACGTR 1 cut(s) 179
BsaJI CCNNGG 5 cut(s) 230, 231, 697, 757, 888
BsaWI WCCGGW 1 cut(s) 164
Bsc4I CCNNNNNNNGG 1 cut(s) 962
Bse1I ACTGG 1 cut(s) 1040
Bse3DI GCAATG 2 cut(s) 103, 666
BseBI CCWGG 6 cut(s) 232, 699, 732, 758, 957, 996
BseDI CCNNGG 5 cut(s) 230, 231, 697, 757, 888
BseGI GGATG 4 cut(s) 267, 471, 693, 1045
BseLI CCNNNNNNNGG 1 cut(s) 962
BseMI GCAATG 2 cut(s) 103, 666
BseNI ACTGG 1 cut(s) 1040
BseRI GAGGAG 2 cut(s) 61, 827
BseXI GCAGC 4 cut(s) 113, 302, 640, 1115
BseYI CCCAGC 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 510
BshFI GGCC 1 cut(s) 470
BsiSI CCGG 2 cut(s) 165, 974
BslI CCNNNNNNNGG 1 cut(s) 962
BsmI GAATGC 1 cut(s) 869
BsnI GGCC 1 cut(s) 470
Bsp143I GATC 2 cut(s) 322, 790
Bsp1720I GCTNAGC 1 cut(s) 32
BspACI CCGC 1 cut(s) 508
BspANI GGCC 1 cut(s) 470
BspFNI CGCG 1 cut(s) 510
BspLI GGNNCC 2 cut(s) 237, 624
BspMI ACCTGC 1 cut(s) 922
BspOI GCTAGC 1 cut(s) 378
BspPI GGATC 1 cut(s) 330
BspTI CTTAAG 2 cut(s) 308, 521
BsrDI GCAATG 2 cut(s) 103, 666
BsrI ACTGG 1 cut(s) 1040
BssECI CCNNGG 5 cut(s) 230, 231, 697, 757, 888
BssMI GATC 2 cut(s) 322, 790
BssT1I CCWWGG 1 cut(s) 888
Bst2UI CCWGG 6 cut(s) 232, 699, 732, 758, 957, 996
Bst4CI ACNGT 1 cut(s) 866
BstAFI CTTAAG 2 cut(s) 308, 521
BstBAI YACGTR 1 cut(s) 179
BstC8I GCNNGC 2 cut(s) 16, 376
BstDEI CTNAG 3 cut(s) 32, 204, 896
BstF5I GGATG 4 cut(s) 267, 471, 693, 1045
BstFNI CGCG 1 cut(s) 510
BstHHI GCGC 1 cut(s) 512
BstKTI GATC 2 cut(s) 325, 793
BstMBI GATC 2 cut(s) 322, 790
BstMWI GCNNNNNNNGC 7 cut(s) 11, 197, 290, 451, 677, 759, 1019
BstNI CCWGG 6 cut(s) 232, 699, 732, 758, 957, 996
BstNSI RCATGY 3 cut(s) 18, 985, 1064
BstPAI GACNNNNGTC 1 cut(s) 585
BstSCI CCNGG 7 cut(s) 230, 697, 730, 756, 955, 972, 994
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 4 cut(s) 113, 302, 640, 1115
BsuRI GGCC 1 cut(s) 470
BtgZI GCGATG 1 cut(s) 1084
BtsCI GGATG 4 cut(s) 267, 471, 693, 1045
BtsI GCAGTG 1 cut(s) 1126
BtsIMutI CAGTG 2 cut(s) 871, 1126
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 2 cut(s) 16, 376
CfoI GCGC 1 cut(s) 512
Cfr13I GGNCC 2 cut(s) 545, 953
CseI GACGC 1 cut(s) 1063
Csp6I GTAC 2 cut(s) 939, 1001
CviAII CATG 8 cut(s) 15, 28, 557, 874, 927, 962, 982, 1061
CviQI GTAC 2 cut(s) 939, 1001
DdeI CTNAG 3 cut(s) 32, 204, 896
DpnI GATC 2 cut(s) 324, 792
DpnII GATC 2 cut(s) 322, 790
DraI TTTAAA 1 cut(s) 724
DraIII CACNNNGTG 1 cut(s) 560
EaeI YGGCCR 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 888
Eco32I GATATC 1 cut(s) 631
Eco47I GGWCC 2 cut(s) 545, 953
Eco57I CTGAAG 1 cut(s) 582
EcoRII CCWGG 6 cut(s) 230, 697, 730, 756, 955, 994
EcoRV GATATC 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 888
EcoT22I ATGCAT 1 cut(s) 1066
ErhI CCWWGG 1 cut(s) 888
FaeI CATG 8 cut(s) 18, 31, 560, 877, 930, 965, 985, 1064
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FatI CATG 8 cut(s) 14, 27, 556, 873, 926, 961, 981, 1060
FauNDI CATATG 1 cut(s) 1050
Fnu4HI GCNGC 4 cut(s) 102, 291, 654, 1104
FokI GGATG 4 cut(s) 274, 478, 680, 1052
Fsp4HI GCNGC 4 cut(s) 102, 291, 654, 1104
FspBI CTAG 3 cut(s) 194, 375, 902
GlaI GCGC 1 cut(s) 511
GluI GCNGC 4 cut(s) 102, 291, 654, 1104
GsaI CCCAGC 1 cut(s) 297
GsuI CTGGAG 2 cut(s) 714, 1017
HaeIII GGCC 1 cut(s) 470
HapII CCGG 2 cut(s) 165, 974
HgaI GACGC 1 cut(s) 1063
HhaI GCGC 1 cut(s) 512
Hin1II CATG 8 cut(s) 18, 31, 560, 877, 930, 965, 985, 1064
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HindIII AAGCTT 3 cut(s) 518, 1005, 1109
HinfI GANTC 3 cut(s) 149, 586, 1029
HpaII CCGG 2 cut(s) 165, 974
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 1 cut(s) 941
Hpy188I TCNGA 6 cut(s) 87, 160, 256, 545, 601, 801
Hpy188III TCNNGA 4 cut(s) 194, 326, 626, 1094
Hpy8I GTNNAC 1 cut(s) 941
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 1 cut(s) 889
HpyCH4III ACNGT 1 cut(s) 866
HpyCH4IV ACGT 1 cut(s) 178
HpyCH4V TGCA 7 cut(s) 101, 776, 854, 869, 1064, 1119, 1124
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 197, 290, 451, 677, 759, 1019
HpyF3I CTNAG 3 cut(s) 32, 204, 896
HpySE526I ACGT 1 cut(s) 178
Hsp92II CATG 8 cut(s) 18, 31, 560, 877, 930, 965, 985, 1064
HspAI GCGC 1 cut(s) 510
Kzo9I GATC 2 cut(s) 322, 790
LmnI GCTCC 4 cut(s) 41, 74, 213, 733
Lsp1109I GCAGC 4 cut(s) 113, 302, 640, 1115
LweI GCATC 1 cut(s) 113
MaeI CTAG 3 cut(s) 194, 375, 902
MaeII ACGT 1 cut(s) 178
MaeIII GTNAC 3 cut(s) 119, 528, 838
MalI GATC 2 cut(s) 324, 792
MboI GATC 2 cut(s) 322, 790
MboII GAAGA 7 cut(s) 38, 71, 74, 218, 409, 588, 874
MfeI CAATTG 1 cut(s) 807
MlsI TGGCCA 1 cut(s) 470
MluCI AATT 5 cut(s) 88, 140, 616, 807, 1089
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 1 cut(s) 595
MmeI TCCRAC 2 cut(s) 183, 279
MnlI CCTC 4 cut(s) 82, 190, 519, 805
Mox20I TGGCCA 1 cut(s) 470
Mph1103I ATGCAT 1 cut(s) 1066
MscI TGGCCA 1 cut(s) 470
MslI CAYNNNNRTG 2 cut(s) 834, 948
Msp20I TGGCCA 1 cut(s) 470
MspA1I CMGCKG 1 cut(s) 293
MspCI CTTAAG 2 cut(s) 308, 521
MspI CCGG 2 cut(s) 165, 974
MspR9I CCNGG 7 cut(s) 232, 699, 732, 758, 957, 974, 996
MunI CAATTG 1 cut(s) 807
Mva1269I GAATGC 1 cut(s) 869
MvaI CCWGG 6 cut(s) 232, 699, 732, 758, 957, 996
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 7 cut(s) 11, 197, 290, 451, 677, 759, 1019
NciI CCSGG 1 cut(s) 974
NdeI CATATG 1 cut(s) 1050
NdeII GATC 2 cut(s) 322, 790
NheI GCTAGC 1 cut(s) 374
NlaIII CATG 8 cut(s) 18, 31, 560, 877, 930, 965, 985, 1064
NlaIV GGNNCC 2 cut(s) 237, 624
NmuCI GTSAC 2 cut(s) 528, 838
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 3 cut(s) 18, 985, 1064
PaeI GCATGC 1 cut(s) 18
PasI CCCWGGG 1 cut(s) 231
PciI ACATGT 1 cut(s) 981
PctI GAATGC 1 cut(s) 869
PfeI GAWTC 2 cut(s) 149, 1029
PflMI CCANNNNNTGG 1 cut(s) 962
PfoI TCCNGGA 1 cut(s) 955
PkrI GCNGC 4 cut(s) 103, 292, 655, 1105
PleI GAGTC 1 cut(s) 594
PpsI GAGTC 1 cut(s) 594
Ppu21I YACGTR 1 cut(s) 179
PscI ACATGT 1 cut(s) 981
PshAI GACNNNNGTC 1 cut(s) 585
PsiI TTATAA 1 cut(s) 738
Psp6I CCWGG 6 cut(s) 230, 697, 730, 756, 955, 994
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 6 cut(s) 230, 697, 730, 756, 955, 994
PspN4I GGNNCC 2 cut(s) 237, 624
PspPI GGNCC 2 cut(s) 545, 953
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 2 cut(s) 940, 1002
RsaNI GTAC 2 cut(s) 939, 1001
RseI CAYNNNNRTG 2 cut(s) 834, 948
SatI GCNGC 4 cut(s) 102, 291, 654, 1104
Sau3AI GATC 2 cut(s) 322, 790
Sau96I GGNCC 2 cut(s) 545, 953
SchI GAGTC 1 cut(s) 595
ScrFI CCNGG 7 cut(s) 232, 699, 732, 758, 957, 974, 996
SfaNI GCATC 1 cut(s) 113
SinI GGWCC 2 cut(s) 545, 953
SmiMI CAYNNNNRTG 2 cut(s) 834, 948
SmlI CTYRAG 3 cut(s) 308, 521, 583
SmoI CTYRAG 3 cut(s) 308, 521, 583
SphI GCATGC 1 cut(s) 18
Sse9I AATT 5 cut(s) 88, 140, 616, 807, 1089
SsiI CCGC 1 cut(s) 508
SspI AATATT 1 cut(s) 414
SspMI CTAG 3 cut(s) 194, 375, 902
StyD4I CCNGG 7 cut(s) 230, 697, 730, 756, 955, 972, 994
StyI CCWWGG 1 cut(s) 888
TaaI ACNGT 1 cut(s) 866
TaiI ACGT 1 cut(s) 181
TaqI TCGA 1 cut(s) 247
TasI AATT 5 cut(s) 88, 140, 616, 807, 1089
TatI WGTACW 1 cut(s) 1000
TfiI GAWTC 2 cut(s) 149, 1029
TscAI CASTG 2 cut(s) 871, 1126
TseFI GTSAC 2 cut(s) 528, 838
TseI GCWGC 4 cut(s) 101, 290, 653, 1103
Tsp45I GTSAC 2 cut(s) 528, 838
TspDTI ATGAA 4 cut(s) 16, 498, 529, 862
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 2 cut(s) 871, 1126
Van91I CCANNNNNTGG 1 cut(s) 962
Vha464I CTTAAG 2 cut(s) 308, 521
VpaK11BI GGWCC 2 cut(s) 545, 953
XapI RAATTY 1 cut(s) 88
XbaI TCTAGA 1 cut(s) 193
XceI RCATGY 3 cut(s) 18, 985, 1064
XspI CTAG 3 cut(s) 194, 375, 902
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.