Rw3G011650

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000911
Physical Location & Seq
Forward (+)
11387780 .. 11391462
3683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G011650.1

Sequence Viewer

Length: 1350 bp
ATGGCTGCTTCAATCTCAACAACCATCGGAGCTGTTACCAGAGCACCGTTGAAGTTGAATGGGGCAGGTGCTTCATCTTCATTTCCGAGTTCTGCTTTCTTAGGCAAGAATTTGAGCTCCAAAGTCACCCAAAGAAGGACTGCATCTCGGAATTTCAAAGTTGCTGCAGAATACGATGAGGAAAAGCAGACCTCAAAGGACAGATGGGGAGGACTTGCTTTCGACACTTCAGATGATCAGCAGGACATCACCAGAGGAAAGGGAATGGTGGACACTCTGTTCCAAGCTCCTACTGGTAGTGGGACTCACCATGCCATCATGAGCTCCTATGATTACATCAGCACTGGTCTTCGCCAGTACAGTTTGGATAACGTTGTGGATGGCTTCTACATTGCTCCAGCTTTCATGGATAAGCTTGTTGTTCACATTACTAAGAACTTCTTGAACCTGCCAAACATCAAGGTTCCTCTTATTTTGGGTGTCTGGGGAGGCAAAGGTCAAGGAAAGTCATTCCAGTGTGAGCTTGTTTTTGCCAAGATGGGAATCAACCCTATTATGATGAGTGCTGGAGAACTGGAGAGTGGAAATGCAGGGGAGCCAGCAAAACTGATCAGGCAAAGGTATCGTGAAGCAGCAGACATCATCAGCAAGGGCAAAATGTGCTGCCTCTTCATTAATGATTTGGATGCAGGAGCTGGAAGGCTTGGTGGAACTACTCAATACACCGTCAACAATCAAATGGTGAATGCTACCCTTATGAACATTGCAGACAATCCGACCAATGTCCAACTCCCTGGTATGTACAACAAGCAGGAGAATCCCCGTGTTCCCATTATAGTCACCGGAAATGACTTCTCCACATTGTATGCCCCTCTCATCCGAGATGGACGTATGGAGAAATTCTACTGGGCGCCTACTCGTGAAGACCGAATTGGTGTCTGCACAGGAATTTTCAAGACTGATAATATTCCTACTGATGACATTGTCAAGCTTGTTGACACCTTCCCTGGTCAGTCAATTGACTTCTTCGGTGCCCTCAGGGCAAGAATTTATGATGATGAAGTGAGGAAGTGGGTTGCAAGTGTTGGAGTAGAAGGTGTTGGGAAGAGGCTTGTTAACTCAAAAGAAGGACCCCCAACTTTCAATCAGCCAAAGATGACACTTGAGAAGCTCCTCGAGTATGGAAACATGCTTGTGCAAGAGCAGGAGAATGTGAAGAGAGTCCAACTGTCTGACAGATACTTGAAAGAGGCAGCTCTCGGAGACGCCAATGACGATGCCATCAAGAGTGGCAACTTCTATGCACCATCTCACTTCAAAAGGGATCCTACCCTTAATTCAAGCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

449

Amino Acids

49.3

Weight (kDa)

8.45

Isoelectric Point (pI)

32.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 161 - 304 7.9e-17 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 307 - 402 1.8e-41 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 56
Acc36I ACCTGC 2 cut(s) 56, 456
AccB1I GGYRCC 2 cut(s) 910, 1031
AclI AACGTT 1 cut(s) 372
AclWI GGATC 2 cut(s) 1319, 1332
AcsI RAATTY 5 cut(s) 109, 151, 899, 948, 1047
AcuI CTGAAG 1 cut(s) 213
AcyI GRCGYC 2 cut(s) 911, 1266
AfaI GTAC 2 cut(s) 359, 803
AfiI CCNNNNNNNGG 1 cut(s) 135
AjnI CCWGG 2 cut(s) 793, 1006
AjuI GAANNNNNNNTTGG 2 cut(s) 915, 947
AloI GAACNNNNNNTCC 2 cut(s) 263, 295
Alw21I GWGCWC 3 cut(s) 46, 119, 326
Alw26I GTCTC 1 cut(s) 1257
AlwI GGATC 2 cut(s) 1319, 1332
AlwNI CAGNNNCTG 1 cut(s) 695
Ama87I CYCGRG 1 cut(s) 1175
ApeKI GCWGC 5 cut(s) 5, 164, 632, 663, 1253
ApoI RAATTY 5 cut(s) 109, 151, 899, 948, 1047
AseI ATTAAT 1 cut(s) 675
AspLEI GCGC 1 cut(s) 913
AspS9I GGNCC 1 cut(s) 1130
AsuHPI GGTGA 5 cut(s) 118, 241, 299, 754, 832
AvaI CYCGRG 1 cut(s) 1175
AvaII GGWCC 1 cut(s) 1130
AxyI CCTNAGG 1 cut(s) 1037
BaeGI GKGCMC 1 cut(s) 1036
BamHI GGATCC 1 cut(s) 1324
BanI GGYRCC 2 cut(s) 910, 1031
BanII GRGCYC 2 cut(s) 119, 326
BauI CACGAG 1 cut(s) 918
BbsI GAAGAC 2 cut(s) 341, 930
Bbv12I GWGCWC 3 cut(s) 46, 119, 326
BbvI GCAGC 4 cut(s) 151, 644, 650, 1265
BccI CCATC 8 cut(s) 32, 198, 323, 374, 532, 878, 1289, 1315
BcgI CGANNNNNNTGC 2 cut(s) 605, 639
BciT130I CCWGG 2 cut(s) 795, 1008
BclI TGATCA 2 cut(s) 235, 609
BcoDI GTCTC 1 cut(s) 1257
BfmI CTRYAG 1 cut(s) 165
BfoI RGCGCY 1 cut(s) 914
BfuAI ACCTGC 2 cut(s) 56, 456
BglI GCCNNNNNGGC 1 cut(s) 1040
BisI GCNGC 5 cut(s) 6, 165, 633, 664, 1254
BlsI GCNGC 5 cut(s) 7, 166, 634, 665, 1255
Bme1390I CCNGG 2 cut(s) 795, 1008
Bme18I GGWCC 1 cut(s) 1130
BmeT110I CYCGRG 1 cut(s) 1175
BmgT120I GGNCC 1 cut(s) 1130
BmiI GGNNCC 6 cut(s) 465, 597, 912, 1033, 1132, 1326
BmrFI CCNGG 2 cut(s) 795, 1008
BmrI ACTGGG 1 cut(s) 916
BmsI GCATC 3 cut(s) 152, 676, 1267
BmuI ACTGGG 1 cut(s) 916
BoxI GACNNNNGTC 1 cut(s) 782
BpiI GAAGAC 2 cut(s) 341, 930
BpmI CTGGAG 3 cut(s) 381, 588, 596
BpuEI CTTGAG 1 cut(s) 1184
BsaBI GATNNNNATC 1 cut(s) 542
BsaHI GRCGYC 2 cut(s) 911, 1266
BsaJI CCNNGG 2 cut(s) 793, 1006
BsaWI WCCGGW 1 cut(s) 842
Bsc4I CCNNNNNNNGG 1 cut(s) 135
Bse1I ACTGG 6 cut(s) 298, 349, 355, 514, 579, 911
Bse21I CCTNAGG 1 cut(s) 1037
Bse3DI GCAATG 2 cut(s) 390, 762
Bse8I GATNNNNATC 1 cut(s) 542
BseBI CCWGG 2 cut(s) 795, 1008
BseDI CCNNGG 2 cut(s) 793, 1006
BseGI GGATG 3 cut(s) 385, 691, 876
BseJI GATNNNNATC 1 cut(s) 542
BseLI CCNNNNNNNGG 1 cut(s) 135
BseMI GCAATG 2 cut(s) 390, 762
BseMII CTCAG 1 cut(s) 1051
BseNI ACTGG 6 cut(s) 298, 349, 355, 514, 579, 911
BseRI GAGGAG 1 cut(s) 1163
BseSI GKGCMC 1 cut(s) 1036
BseXI GCAGC 4 cut(s) 151, 644, 650, 1265
BsgI GTGCAG 1 cut(s) 925
BshNI GGYRCC 2 cut(s) 910, 1031
BsiHKAI GWGCWC 3 cut(s) 46, 119, 326
BsiHKCI CYCGRG 1 cut(s) 1175
BsiSI CCGG 1 cut(s) 843
BslFI GGGAC 1 cut(s) 316
BslI CCNNNNNNNGG 1 cut(s) 135
BsmAI GTCTC 1 cut(s) 1257
BsmBI CGTCTC 1 cut(s) 1257
BsmFI GGGAC 1 cut(s) 316
BsmI GAATGC 1 cut(s) 751
BsoBI CYCGRG 1 cut(s) 1175
Bsp1286I GDGCHC 4 cut(s) 46, 119, 326, 1036
Bsp1407I TGTACA 1 cut(s) 801
Bsp143I GATC 3 cut(s) 235, 609, 1324
BspCNI CTCAG 1 cut(s) 1050
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 6 cut(s) 465, 597, 912, 1033, 1132, 1326
BspMAI CTGCAG 1 cut(s) 169
BspMI ACCTGC 2 cut(s) 56, 456
BspPI GGATC 2 cut(s) 1319, 1332
BspT107I GGYRCC 2 cut(s) 910, 1031
BsrDI GCAATG 2 cut(s) 390, 762
BsrGI TGTACA 1 cut(s) 801
BsrI ACTGG 6 cut(s) 298, 349, 355, 514, 579, 911
BssECI CCNNGG 2 cut(s) 793, 1006
BssMI GATC 3 cut(s) 235, 609, 1324
BssNI GRCGYC 2 cut(s) 911, 1266
BssSI CACGAG 1 cut(s) 918
Bst2BI CACGAG 1 cut(s) 918
Bst2UI CCWGG 2 cut(s) 795, 1008
Bst4CI ACNGT 4 cut(s) 48, 362, 727, 1230
Bst6I CTCTTC 3 cut(s) 674, 1100, 1211
BstACI GRCGYC 2 cut(s) 911, 1266
BstAPI GCANNNNNTGC 1 cut(s) 660
BstAUI TGTACA 1 cut(s) 801
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 3 cut(s) 100, 432, 1037
BstF5I GGATG 3 cut(s) 385, 691, 876
BstH2I RGCGCY 1 cut(s) 914
BstHHI GCGC 1 cut(s) 913
BstKTI GATC 3 cut(s) 238, 612, 1327
BstMAI GTCTC 1 cut(s) 1257
BstMBI GATC 3 cut(s) 235, 609, 1324
BstMWI GCNNNNNNNGC 2 cut(s) 660, 1040
BstNI CCWGG 2 cut(s) 795, 1008
BstNSI RCATGY 1 cut(s) 1192
BstPAI GACNNNNGTC 1 cut(s) 782
BstSCI CCNGG 2 cut(s) 793, 1006
BstSFI CTRYAG 1 cut(s) 165
BstSLI GKGCMC 1 cut(s) 1036
BstV1I GCAGC 4 cut(s) 151, 644, 650, 1265
BstV2I GAAGAC 2 cut(s) 341, 930
BstX2I RGATCY 1 cut(s) 1324
BstXI CCANNNNNNTGG 1 cut(s) 794
BstYI RGATCY 1 cut(s) 1324
Bsu36I CCTNAGG 1 cut(s) 1037
BtsCI GGATG 3 cut(s) 385, 691, 876
BtsIMutI CAGTG 2 cut(s) 342, 521
BveI ACCTGC 2 cut(s) 56, 456
Cac8I GCNNGC 1 cut(s) 600
CaiI CAGNNNCTG 1 cut(s) 695
CciI TCATGA 1 cut(s) 318
CfoI GCGC 1 cut(s) 913
Cfr13I GGNCC 1 cut(s) 1130
CseI GACGC 1 cut(s) 1274
Csp6I GTAC 2 cut(s) 358, 802
CviAII CATG 4 cut(s) 311, 319, 406, 1189
CviQI GTAC 2 cut(s) 358, 802
DdeI CTNAG 3 cut(s) 100, 432, 1037
DinI GGCGCC 1 cut(s) 912
DpnI GATC 3 cut(s) 237, 611, 1326
DpnII GATC 3 cut(s) 235, 609, 1324
Eam1104I CTCTTC 3 cut(s) 674, 1100, 1211
EarI CTCTTC 3 cut(s) 674, 1100, 1211
Ecl136II GAGCTC 2 cut(s) 117, 324
Eco24I GRGCYC 2 cut(s) 119, 326
Eco47I GGWCC 1 cut(s) 1130
Eco53kI GAGCTC 2 cut(s) 117, 324
Eco57I CTGAAG 1 cut(s) 213
Eco81I CCTNAGG 1 cut(s) 1037
Eco88I CYCGRG 1 cut(s) 1175
EcoICRI GAGCTC 2 cut(s) 117, 324
EcoO109I RGGNCCY 1 cut(s) 1130
EcoRII CCWGG 2 cut(s) 793, 1006
EcoT38I GRGCYC 2 cut(s) 119, 326
EgeI GGCGCC 1 cut(s) 912
EheI GGCGCC 1 cut(s) 912
Esp3I CGTCTC 1 cut(s) 1257
FaeI CATG 4 cut(s) 314, 322, 409, 1192
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 1 cut(s) 316
FatI CATG 4 cut(s) 310, 318, 405, 1188
FbaI TGATCA 2 cut(s) 235, 609
Fnu4HI GCNGC 5 cut(s) 6, 165, 633, 664, 1254
FokI GGATG 3 cut(s) 392, 698, 863
FriOI GRGCYC 2 cut(s) 119, 326
Fsp4HI GCNGC 5 cut(s) 6, 165, 633, 664, 1254
GlaI GCGC 1 cut(s) 912
GluI GCNGC 5 cut(s) 6, 165, 633, 664, 1254
GsuI CTGGAG 3 cut(s) 381, 588, 596
HaeII RGCGCY 1 cut(s) 914
HapII CCGG 1 cut(s) 843
HgaI GACGC 1 cut(s) 1274
HhaI GCGC 1 cut(s) 913
Hin1I GRCGYC 2 cut(s) 911, 1266
Hin1II CATG 4 cut(s) 314, 322, 409, 1192
Hin6I GCGC 1 cut(s) 911
HinP1I GCGC 1 cut(s) 911
HincII GTYRAC 3 cut(s) 730, 997, 1117
HindII GTYRAC 3 cut(s) 730, 997, 1117
HindIII AAGCTT 2 cut(s) 413, 989
HinfI GANTC 4 cut(s) 304, 543, 817, 1221
HpaI GTTAAC 1 cut(s) 1117
HpaII CCGG 1 cut(s) 843
HphI GGTGA 5 cut(s) 118, 241, 299, 754, 832
Hpy166II GTNNAC 5 cut(s) 271, 424, 730, 997, 1117
Hpy188I TCNGA 8 cut(s) 29, 87, 150, 232, 777, 881, 1234, 1262
Hpy188III TCNNGA 6 cut(s) 319, 442, 626, 920, 955, 1285
Hpy8I GTNNAC 5 cut(s) 271, 424, 730, 997, 1117
HpyAV CCTTC 5 cut(s) 129, 693, 1012, 1088, 1121
HpyCH4III ACNGT 4 cut(s) 48, 362, 727, 1230
HpyCH4IV ACGT 2 cut(s) 372, 889
HpyCH4V TGCA 9 cut(s) 143, 167, 590, 689, 767, 942, 1079, 1198, 1304
HpyF10VI GCNNNNNNNGC 2 cut(s) 660, 1040
HpyF3I CTNAG 3 cut(s) 100, 432, 1037
HpySE526I ACGT 2 cut(s) 372, 889
Hsp92I GRCGYC 2 cut(s) 911, 1266
Hsp92II CATG 4 cut(s) 314, 322, 409, 1192
HspAI GCGC 1 cut(s) 911
KasI GGCGCC 1 cut(s) 910
Ksp22I TGATCA 2 cut(s) 235, 609
KspAI GTTAAC 1 cut(s) 1117
Kzo9I GATC 3 cut(s) 235, 609, 1324
LmnI GCTCC 9 cut(s) 29, 122, 292, 329, 400, 595, 692, 1176, 1349
Lsp1109I GCAGC 4 cut(s) 151, 644, 650, 1265
LweI GCATC 3 cut(s) 152, 676, 1267
MaeII ACGT 2 cut(s) 372, 889
MaeIII GTNAC 3 cut(s) 34, 124, 838
MalI GATC 3 cut(s) 237, 611, 1326
MboI GATC 3 cut(s) 235, 609, 1324
MboII GAAGA 7 cut(s) 69, 341, 661, 935, 1018, 1117, 1228
MfeI CAATTG 1 cut(s) 1017
MflI RGATCY 1 cut(s) 1324
MhlI GDGCHC 4 cut(s) 46, 119, 326, 1036
MluCI AATT 8 cut(s) 109, 151, 899, 930, 948, 1017, 1047, 1336
Mly113I GGCGCC 1 cut(s) 911
MlyI GAGTC 2 cut(s) 298, 1230
MmeI TCCRAC 4 cut(s) 800, 811, 1066, 1249
MseI TTAA 3 cut(s) 675, 1116, 1335
MslI CAYNNNNRTG 2 cut(s) 514, 1193
MspI CCGG 1 cut(s) 843
MspR9I CCNGG 2 cut(s) 795, 1008
MunI CAATTG 1 cut(s) 1017
Mva1269I GAATGC 1 cut(s) 751
MvaI CCWGG 2 cut(s) 795, 1008
MwoI GCNNNNNNNGC 2 cut(s) 660, 1040
NarI GGCGCC 1 cut(s) 911
NdeII GATC 3 cut(s) 235, 609, 1324
NlaIII CATG 4 cut(s) 314, 322, 409, 1192
NlaIV GGNNCC 6 cut(s) 465, 597, 912, 1033, 1132, 1326
NmuCI GTSAC 2 cut(s) 124, 838
NspI RCATGY 1 cut(s) 1192
PaeR7I CTCGAG 1 cut(s) 1175
PagI TCATGA 1 cut(s) 318
PaqCI CACCTGC 1 cut(s) 56
PcsI WCGNNNNNNNCGW 2 cut(s) 925, 1272
PctI GAATGC 1 cut(s) 751
PfeI GAWTC 2 cut(s) 543, 817
PflFI GACNNNGTC 1 cut(s) 983
PkrI GCNGC 5 cut(s) 7, 166, 634, 665, 1255
PleI GAGTC 2 cut(s) 298, 1229
PluTI GGCGCC 1 cut(s) 914
PpsI GAGTC 2 cut(s) 298, 1229
PpuMI RGGWCCY 1 cut(s) 1130
PshAI GACNNNNGTC 1 cut(s) 782
PshBI ATTAAT 1 cut(s) 675
Psp124BI GAGCTC 2 cut(s) 119, 326
Psp1406I AACGTT 1 cut(s) 372
Psp5II RGGWCCY 1 cut(s) 1130
Psp6I CCWGG 2 cut(s) 793, 1006
PspGI CCWGG 2 cut(s) 793, 1006
PspN4I GGNNCC 6 cut(s) 465, 597, 912, 1033, 1132, 1326
PspPI GGNCC 1 cut(s) 1130
PspPPI RGGWCCY 1 cut(s) 1130
PspXI VCTCGAGB 1 cut(s) 1175
PstI CTGCAG 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 695
PsuI RGATCY 1 cut(s) 1324
PsyI GACNNNGTC 1 cut(s) 983
RsaI GTAC 2 cut(s) 359, 803
RsaNI GTAC 2 cut(s) 358, 802
RseI CAYNNNNRTG 2 cut(s) 514, 1193
SacI GAGCTC 2 cut(s) 119, 326
SaqAI TTAA 3 cut(s) 675, 1116, 1335
SatI GCNGC 5 cut(s) 6, 165, 633, 664, 1254
Sau3AI GATC 3 cut(s) 235, 609, 1324
Sau96I GGNCC 1 cut(s) 1130
SchI GAGTC 2 cut(s) 298, 1230
ScrFI CCNGG 2 cut(s) 795, 1008
SduI GDGCHC 4 cut(s) 46, 119, 326, 1036
SfaNI GCATC 3 cut(s) 152, 676, 1267
SfcI CTRYAG 1 cut(s) 165
SfoI GGCGCC 1 cut(s) 912
Sfr274I CTCGAG 1 cut(s) 1175
SinI GGWCC 1 cut(s) 1130
SlaI CTCGAG 1 cut(s) 1175
SmiMI CAYNNNNRTG 2 cut(s) 514, 1193
SmlI CTYRAG 2 cut(s) 1163, 1175
SmoI CTYRAG 2 cut(s) 1163, 1175
Sse9I AATT 8 cut(s) 109, 151, 899, 930, 948, 1017, 1047, 1336
SspDI GGCGCC 1 cut(s) 910
SspI AATATT 1 cut(s) 967
SstI GAGCTC 2 cut(s) 119, 326
StyD4I CCNGG 2 cut(s) 793, 1006
TaaI ACNGT 4 cut(s) 48, 362, 727, 1230
TaiI ACGT 2 cut(s) 375, 892
TaqI TCGA 2 cut(s) 222, 1176
TaqII GACCGA 1 cut(s) 942
TasI AATT 8 cut(s) 109, 151, 899, 930, 948, 1017, 1047, 1336
TatI WGTACW 2 cut(s) 357, 801
TfiI GAWTC 2 cut(s) 543, 817
Tru1I TTAA 3 cut(s) 675, 1116, 1335
Tru9I TTAA 3 cut(s) 675, 1116, 1335
TscAI CASTG 2 cut(s) 349, 521
TseFI GTSAC 2 cut(s) 124, 838
TseI GCWGC 5 cut(s) 5, 164, 632, 663, 1253
Tsp45I GTSAC 2 cut(s) 124, 838
TspDTI ATGAA 6 cut(s) 63, 69, 394, 661, 773, 1074
TspRI CASTG 2 cut(s) 349, 521
Tth111I GACNNNGTC 1 cut(s) 983
VpaK11BI GGWCC 1 cut(s) 1130
VspI ATTAAT 1 cut(s) 675
XapI RAATTY 5 cut(s) 109, 151, 899, 948, 1047
XceI RCATGY 1 cut(s) 1192
XcmI CCANNNNNNNNNTGG 1 cut(s) 290
XhoI CTCGAG 1 cut(s) 1175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.