Rorug05G0377200

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000911
Physical Location & Seq
Forward (+)
51020613 .. 51021968
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0377200.1

Sequence Viewer

Length: 831 bp
ATGGAAGATGTTGATGAAATGGCAGTGTGCTTCGCTGCGTCTTTGGGGTTGGTATATGCCCGAAGGCCAGTGGATCTCCGACCACCCCGAGGCGAAGGTCTTCGCCGATCCCAGACTTTTTTGGTGGGAAAATTTTTGATTTCTCGGGCTTTCAACAAGAGGTCCTTCATAGGGATGTTCCGACCATGGGGAGGACCATGGGGATTTGATCGTGCTCCGGTAGTGTTGGCATTGTATGATGGCGTCGGAGCTATCCGAGATGTTCCTCTCTCCACCCTAGCGTTTTGGATCAAAGTAAGATCCGGATTCGGGTTGAGATGGATCTCTTGCATCCGGTCATATTTCGCCGGAGTTTTACGTACAGTGGATGATGGCGTTGATGTCCACTTGGATCTCTTCTTCAAGAATCTATATGGGAGGTGTAGAGAGTGTGGTCTCCTCACCCATGTTAGGTTGCCATGCTTTGGAACATCGTTGCCAGAGGTGTTGAGGACAGAAGTTTCACCTGCGCGTTGGAAATTTGGAGAGGGTCCACCTGGTAGAAGTGACACCAACTGCCTGGCCTTCTTTTGTGTTTGGGGAGAGGGTGGTGGATCAGCAGAACAAGTTCTGGAAGAGGCCATTGCTAATCCTAGTTGTGGCATGCGCAGAAAGAGGTTAGCGGACGATGGAGCGTCGCCGAAGAAACTGCGCCTTAGTTCGGTTGTGGGTAGAACCAATTTGGACGCTATGTCTATGGGACTATGCTCTTTGCAACATGTGCAAGAATTTTCTAAGAAGAGAGCAGATAGCCCGAACCCCTCCAAGGGTAAGGAGCCTATAGTTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

276

Amino Acids

30.52

Weight (kDa)

9.43

Isoelectric Point (pI)

57.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 514
Acc16I TGCGCA 1 cut(s) 647
Acc36I ACCTGC 1 cut(s) 514
AccB7I CCANNNNNTGG 1 cut(s) 464
AccII CGCG 1 cut(s) 511
AccIII TCCGGA 1 cut(s) 302
AciI CCGC 1 cut(s) 662
AclWI GGATC 7 cut(s) 81, 102, 294, 296, 329, 399, 601
AcsI RAATTY 3 cut(s) 131, 518, 767
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 1 cut(s) 361
AflIII ACRYGT 1 cut(s) 757
AgsI TTSAA 2 cut(s) 154, 403
AhdI GACNNNNNGTC 1 cut(s) 730
AjnI CCWGG 2 cut(s) 535, 558
AluBI AGCT 1 cut(s) 251
AluI AGCT 1 cut(s) 251
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 1 cut(s) 440
AlwI GGATC 7 cut(s) 81, 102, 294, 296, 329, 399, 601
Ama87I CYCGRG 2 cut(s) 87, 144
Aor13HI TCCGGA 1 cut(s) 302
AoxI GGCC 3 cut(s) 65, 561, 618
ApeKI GCWGC 1 cut(s) 35
ApoI RAATTY 3 cut(s) 131, 518, 767
Asp700I GAANNNNTTC 2 cut(s) 99, 606
AspLEI GCGC 3 cut(s) 511, 648, 693
AspS9I GGNCC 3 cut(s) 162, 194, 530
AsuHPI GGTGA 2 cut(s) 433, 495
AvaI CYCGRG 2 cut(s) 87, 144
AvaII GGWCC 3 cut(s) 162, 194, 530
BbsI GAAGAC 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 1 cut(s) 22
BccI CCATC 4 cut(s) 233, 312, 365, 662
BcgI CGANNNNNNTGC 2 cut(s) 670, 704
BciT130I CCWGG 2 cut(s) 537, 560
BcoDI GTCTC 1 cut(s) 440
BfaI CTAG 2 cut(s) 278, 633
BfmI CTRYAG 1 cut(s) 819
BfuAI ACCTGC 1 cut(s) 514
BisI GCNGC 1 cut(s) 36
BlsI GCNGC 1 cut(s) 37
Bme1390I CCNGG 2 cut(s) 537, 560
Bme18I GGWCC 3 cut(s) 162, 194, 530
BmeRI GACNNNNNGTC 1 cut(s) 730
BmeT110I CYCGRG 2 cut(s) 87, 144
BmgT120I GGNCC 3 cut(s) 162, 194, 530
BmiI GGNNCC 2 cut(s) 531, 816
BmrFI CCNGG 2 cut(s) 537, 560
BmsI GCATC 1 cut(s) 339
BpiI GAAGAC 1 cut(s) 92
BsaAI YACGTR 1 cut(s) 359
BsaHI GRCGYC 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 440
BsaJI CCNNGG 4 cut(s) 88, 185, 197, 804
BsaWI WCCGGW 3 cut(s) 217, 302, 333
Bse1I ACTGG 1 cut(s) 68
Bse3DI GCAATG 1 cut(s) 621
BseAI TCCGGA 1 cut(s) 302
BseBI CCWGG 2 cut(s) 537, 560
BseDI CCNNGG 4 cut(s) 88, 185, 197, 804
BseGI GGATG 3 cut(s) 180, 330, 373
BseMI GCAATG 1 cut(s) 621
BseNI ACTGG 1 cut(s) 68
BseRI GAGGAG 1 cut(s) 428
BseXI GCAGC 1 cut(s) 22
Bsh1236I CGCG 1 cut(s) 511
BshFI GGCC 3 cut(s) 67, 563, 620
BsiHKAI GWGCWC 1 cut(s) 217
BsiHKCI CYCGRG 2 cut(s) 87, 144
BsiSI CCGG 4 cut(s) 218, 303, 334, 348
BslFI GGGAC 1 cut(s) 753
BsmAI GTCTC 1 cut(s) 440
BsmFI GGGAC 1 cut(s) 753
BsnI GGCC 3 cut(s) 67, 563, 620
Bso31I GGTCTC 1 cut(s) 440
BsoBI CYCGRG 2 cut(s) 87, 144
Bsp1286I GDGCHC 1 cut(s) 217
Bsp13I TCCGGA 1 cut(s) 302
Bsp143I GATC 8 cut(s) 73, 107, 208, 288, 299, 321, 391, 593
Bsp19I CCATGG 2 cut(s) 185, 197
BspACI CCGC 1 cut(s) 662
BspANI GGCC 3 cut(s) 67, 563, 620
BspEI TCCGGA 1 cut(s) 302
BspFNI CGCG 1 cut(s) 511
BspLI GGNNCC 2 cut(s) 531, 816
BspMI ACCTGC 1 cut(s) 514
BspPI GGATC 7 cut(s) 81, 102, 294, 296, 329, 399, 601
BspTNI GGTCTC 1 cut(s) 440
BsrDI GCAATG 1 cut(s) 621
BsrI ACTGG 1 cut(s) 68
BssECI CCNNGG 4 cut(s) 88, 185, 197, 804
BssMI GATC 8 cut(s) 73, 107, 208, 288, 299, 321, 391, 593
BssNI GRCGYC 1 cut(s) 243
BssT1I CCWWGG 3 cut(s) 185, 197, 804
Bst2UI CCWGG 2 cut(s) 537, 560
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 3 cut(s) 401, 609, 773
BstACI GRCGYC 1 cut(s) 243
BstAPI GCANNNNNTGC 1 cut(s) 760
BstBAI YACGTR 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 644
BstDEI CTNAG 3 cut(s) 695, 774, 828
BstDSI CCRYGG 2 cut(s) 185, 197
BstF5I GGATG 3 cut(s) 180, 330, 373
BstFNI CGCG 1 cut(s) 511
BstHHI GCGC 3 cut(s) 511, 648, 693
BstKTI GATC 8 cut(s) 76, 110, 211, 291, 302, 324, 394, 596
BstMAI GTCTC 1 cut(s) 440
BstMBI GATC 8 cut(s) 73, 107, 208, 288, 299, 321, 391, 593
BstMWI GCNNNNNNNGC 1 cut(s) 760
BstNI CCWGG 2 cut(s) 537, 560
BstNSI RCATGY 2 cut(s) 646, 761
BstSCI CCNGG 2 cut(s) 535, 558
BstSFI CTRYAG 1 cut(s) 819
BstSNI TACGTA 1 cut(s) 359
BstUI CGCG 1 cut(s) 511
BstV1I GCAGC 1 cut(s) 22
BstV2I GAAGAC 1 cut(s) 92
BstX2I RGATCY 4 cut(s) 73, 299, 321, 391
BstXI CCANNNNNNTGG 1 cut(s) 559
BstYI RGATCY 4 cut(s) 73, 299, 321, 391
BsuRI GGCC 3 cut(s) 67, 563, 620
BtgI CCRYGG 2 cut(s) 185, 197
BtsCI GGATG 3 cut(s) 180, 330, 373
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 3 cut(s) 30, 75, 369
BveI ACCTGC 1 cut(s) 514
Cac8I GCNNGC 1 cut(s) 644
CfoI GCGC 3 cut(s) 511, 648, 693
Cfr13I GGNCC 3 cut(s) 162, 194, 530
CseI GACGC 4 cut(s) 27, 232, 663, 734
CsiI ACCWGGT 1 cut(s) 535
Csp6I GTAC 1 cut(s) 360
CviAII CATG 6 cut(s) 186, 198, 446, 459, 643, 758
CviJI RGCY 7 cut(s) 67, 149, 251, 563, 620, 792, 817
CviKI_1 RGCY 7 cut(s) 67, 149, 251, 563, 620, 792, 817
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 3 cut(s) 695, 774, 828
DpnI GATC 8 cut(s) 75, 109, 210, 290, 301, 323, 393, 595
DpnII GATC 8 cut(s) 73, 107, 208, 288, 299, 321, 391, 593
DriI GACNNNNNGTC 1 cut(s) 730
Eam1104I CTCTTC 3 cut(s) 401, 609, 773
Eam1105I GACNNNNNGTC 1 cut(s) 730
EarI CTCTTC 3 cut(s) 401, 609, 773
Eco105I TACGTA 1 cut(s) 359
Eco130I CCWWGG 3 cut(s) 185, 197, 804
Eco31I GGTCTC 1 cut(s) 440
Eco47I GGWCC 3 cut(s) 162, 194, 530
Eco88I CYCGRG 2 cut(s) 87, 144
EcoO109I RGGNCCY 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 535, 558
EcoT14I CCWWGG 3 cut(s) 185, 197, 804
ErhI CCWWGG 3 cut(s) 185, 197, 804
FaeI CATG 6 cut(s) 189, 201, 449, 462, 646, 761
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FaqI GGGAC 1 cut(s) 753
FatI CATG 6 cut(s) 185, 197, 445, 458, 642, 757
Fnu4HI GCNGC 1 cut(s) 36
FokI GGATG 3 cut(s) 187, 317, 380
Fsp4HI GCNGC 1 cut(s) 36
FspBI CTAG 2 cut(s) 278, 633
FspI TGCGCA 1 cut(s) 647
GlaI GCGC 3 cut(s) 510, 647, 692
GluI GCNGC 1 cut(s) 36
HaeIII GGCC 3 cut(s) 67, 563, 620
HapII CCGG 4 cut(s) 218, 303, 334, 348
HgaI GACGC 4 cut(s) 27, 232, 663, 734
HhaI GCGC 3 cut(s) 511, 648, 693
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 6 cut(s) 189, 201, 449, 462, 646, 761
Hin6I GCGC 3 cut(s) 509, 646, 691
HinP1I GCGC 3 cut(s) 509, 646, 691
HinfI GANTC 2 cut(s) 306, 406
HpaII CCGG 4 cut(s) 218, 303, 334, 348
HphI GGTGA 2 cut(s) 433, 495
Hpy166II GTNNAC 2 cut(s) 385, 533
Hpy188I TCNGA 4 cut(s) 80, 182, 248, 257
Hpy188III TCNNGA 3 cut(s) 303, 403, 611
Hpy8I GTNNAC 2 cut(s) 385, 533
Hpy99I CGWCG 2 cut(s) 248, 679
HpyAV CCTTC 4 cut(s) 57, 89, 175, 574
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 1 cut(s) 358
HpyCH4V TGCA 3 cut(s) 330, 754, 763
HpyF10VI GCNNNNNNNGC 1 cut(s) 760
HpyF3I CTNAG 3 cut(s) 695, 774, 828
HpySE526I ACGT 1 cut(s) 358
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 6 cut(s) 189, 201, 449, 462, 646, 761
HspAI GCGC 3 cut(s) 509, 646, 691
Kpn2I TCCGGA 1 cut(s) 302
Kzo9I GATC 8 cut(s) 73, 107, 208, 288, 299, 321, 391, 593
LmnI GCTCC 4 cut(s) 220, 248, 671, 814
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 1 cut(s) 339
MabI ACCWGGT 1 cut(s) 535
MaeI CTAG 2 cut(s) 278, 633
MaeII ACGT 1 cut(s) 358
MaeIII GTNAC 2 cut(s) 545, 823
MalI GATC 8 cut(s) 75, 109, 210, 290, 301, 323, 393, 595
MboI GATC 8 cut(s) 73, 107, 208, 288, 299, 321, 391, 593
MboII GAAGA 7 cut(s) 17, 92, 388, 391, 626, 694, 790
MflI RGATCY 4 cut(s) 73, 299, 321, 391
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 4 cut(s) 131, 518, 718, 767
MmeI TCCRAC 4 cut(s) 103, 205, 226, 494
MroI TCCGGA 1 cut(s) 302
MroXI GAANNNNTTC 2 cut(s) 99, 606
MslI CAYNNNNRTG 1 cut(s) 173
MspI CCGG 4 cut(s) 218, 303, 334, 348
MspR9I CCNGG 2 cut(s) 537, 560
MvaI CCWGG 2 cut(s) 537, 560
MvnI CGCG 1 cut(s) 511
MwoI GCNNNNNNNGC 1 cut(s) 760
NcoI CCATGG 2 cut(s) 185, 197
NdeII GATC 8 cut(s) 73, 107, 208, 288, 299, 321, 391, 593
NlaIII CATG 6 cut(s) 189, 201, 449, 462, 646, 761
NlaIV GGNNCC 2 cut(s) 531, 816
NmuCI GTSAC 1 cut(s) 545
NsbI TGCGCA 1 cut(s) 647
NspI RCATGY 2 cut(s) 646, 761
PaeI GCATGC 1 cut(s) 646
PaqCI CACCTGC 1 cut(s) 514
PciI ACATGT 1 cut(s) 757
PdmI GAANNNNTTC 2 cut(s) 99, 606
PfeI GAWTC 2 cut(s) 306, 406
PflMI CCANNNNNTGG 1 cut(s) 464
PkrI GCNGC 1 cut(s) 37
Ppu21I YACGTR 1 cut(s) 359
PpuMI RGGWCCY 1 cut(s) 162
PscI ACATGT 1 cut(s) 757
Psp5II RGGWCCY 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 535, 558
PspGI CCWGG 2 cut(s) 535, 558
PspN4I GGNNCC 2 cut(s) 531, 816
PspPI GGNCC 3 cut(s) 162, 194, 530
PspPPI RGGWCCY 1 cut(s) 162
PsuI RGATCY 4 cut(s) 73, 299, 321, 391
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
RseI CAYNNNNRTG 1 cut(s) 173
SatI GCNGC 1 cut(s) 36
Sau3AI GATC 8 cut(s) 73, 107, 208, 288, 299, 321, 391, 593
Sau96I GGNCC 3 cut(s) 162, 194, 530
ScrFI CCNGG 2 cut(s) 537, 560
SduI GDGCHC 1 cut(s) 217
SexAI ACCWGGT 1 cut(s) 535
SfaNI GCATC 1 cut(s) 339
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 3 cut(s) 162, 194, 530
SmiMI CAYNNNNRTG 1 cut(s) 173
SnaBI TACGTA 1 cut(s) 359
SphI GCATGC 1 cut(s) 646
Sse9I AATT 4 cut(s) 131, 518, 718, 767
SsiI CCGC 1 cut(s) 662
SspMI CTAG 2 cut(s) 278, 633
StyD4I CCNGG 2 cut(s) 535, 558
StyI CCWWGG 3 cut(s) 185, 197, 804
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 1 cut(s) 361
TasI AATT 4 cut(s) 131, 518, 718, 767
TfiI GAWTC 2 cut(s) 306, 406
TscAI CASTG 3 cut(s) 30, 75, 369
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 2 cut(s) 30, 157
TspRI CASTG 3 cut(s) 30, 75, 369
Van91I CCANNNNNTGG 1 cut(s) 464
VpaK11BI GGWCC 3 cut(s) 162, 194, 530
XapI RAATTY 3 cut(s) 131, 518, 767
XceI RCATGY 2 cut(s) 646, 761
XmnI GAANNNNTTC 2 cut(s) 99, 606
XspI CTAG 2 cut(s) 278, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.