MD03G1086300.v1.1

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000911
Physical Location & Seq
Forward (+)
7059628 .. 7063278
3651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1086300.v1.1.491

Sequence Viewer

Length: 1419 bp
ATGGCCGCCATGCACACAGTCTCGAGCGTCGGAACCGTTAACCGGACGCCAGTGAGCTTGATCAGCTCTGGTTCCGGAAGCTCGGCCTTCTTGGGCACCAGTTTGAAGAAAGTGACCTCAAGAGTCCCCTACCAGAAGGTAGCATCTGCAGGCAACTTAAAAATTAATGCGGAAATCGACGAGAACAAGCAAACCAACCAGGACAGATGGAAAGGCCTTGCCTACGACACTTCCGATGACCAACAAGACATAACAAGAGGAAAAGGTTTGGTTGATTCTGTGTTCCAAGCTCCCCAGGGAAGTGGAACTCACTTTGCTGTCATGAGCTCATACGAATACATCAGCACCGGACTTCGAGAATACAACTTTGACAACAACATGGATGGGTTTTACATCGCTCCGGCTTTCATGGACAAGCTTGTGGTTCACATAACCAAAAACTTCATGAGCCTGCCTAACATTAAGATTCCTCTCATTCTTGGTATATGGGGAGGAAAAGGTCAGGGAAAATCCTTCCAGTGTGAGCTTGTGTTTGCCAAGATGGGAATTACCCCCATCATGATGAGTGCTGGAGAATTAGAAAGCGGGAACGCAGGAGAGCCCGCGAAACTGATCAGGCAAAGGTACCGTGAGGCTGCAGACATCATCAGGAAAGGAAAAATGTGTGCCCTCTTCATCAATGATCTCGATGCAGGTGCCGGTAGGATGGGTGGAACCACACAGTACACTGTGAACAACCAGATGGTGAATGCTACCCTCATGAACATTGCTGATAACCCGACAAACGTGCAGCTTCCTGGTATGTACAACAAGGAAGAGAATCCTAGAGTCCCCATTATAGTCACTGGTAACGATTTCTCCACATTGTATGCCCCTCTCATTCGTGACGGTCGTATGGAGAAGTTCTACTGGGCTCCCACTAGGGAAGACCGGATCGGTGTTTGCACAGGAATCTTCAAGCCTGACAATGTGTCCCATGAAGATGTTGTCAAGATTGTTGACACATTCCCAGGTCAATCCATTGATTTCTTCGGTGCTCTGAGGGCTCGAGTTTACGATGATGAAGTGAGGAAGTGGGTATCGGGTGTTGGTATCAACGGCATTGGGAAGAAGCTTGTGAACTCGAAAGAAGGTCCCCCGACTTTCGAGCAACCTAAGATGACTTTGGAGAAGCTCCTTAACTATGGTCAAATGCTTGTTCAGGAGCAAGACAATGTGAAGAGAGTCCAACTGGCTGACAAGTACTTGTCTGATGCAGCCCTCGGAGATGCAAACCAGGACTCCATTAACAGAGGAGAATTCTATGGAAAGGCGGCCCAACAAGTTAAAGTACCAGTCGCAGAAGGGTGCACTGATCCAACAGCTGCAAACTTTGACCCAACAGCAAGAAGTGACAATGGTAGCTGTCAGTACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

473

Amino Acids

51.74

Weight (kDa)

6.2

Isoelectric Point (pI)

30.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 162 - 305 1.7e-16 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 308 - 403 9.5e-41 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 683
Acc36I ACCTGC 1 cut(s) 683
Acc65I GGTACC 1 cut(s) 624
AccB1I GGYRCC 3 cut(s) 95, 624, 695
AccII CGCG 1 cut(s) 605
AccIII TCCGGA 1 cut(s) 74
AciI CCGC 5 cut(s) 6, 170, 585, 603, 1313
AclWI GGATC 2 cut(s) 941, 1349
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 1298
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 6 cut(s) 626, 725, 806, 1244, 1332, 1412
AfiI CCNNNNNNNGG 1 cut(s) 42
AgsI TTSAA 2 cut(s) 106, 958
AhdI GACNNNNNGTC 1 cut(s) 970
AjnI CCWGG 5 cut(s) 198, 294, 796, 1009, 1275
Alw21I GWGCWC 3 cut(s) 329, 1039, 1352
Alw26I GTCTC 1 cut(s) 25
Alw44I GTGCAC 1 cut(s) 1348
AlwI GGATC 2 cut(s) 941, 1349
Ama87I CYCGRG 2 cut(s) 22, 1047
Aor13HI TCCGGA 1 cut(s) 74
AoxI GGCC 4 cut(s) 3, 84, 214, 1314
ApaLI GTGCAC 1 cut(s) 1348
ApeKI GCWGC 4 cut(s) 635, 790, 1256, 1364
ApoI RAATTY 1 cut(s) 1298
AseI ATTAAT 1 cut(s) 165
Asp718I GGTACC 1 cut(s) 624
AspS9I GGNCC 2 cut(s) 1133, 1315
AsuHPI GGTGA 1 cut(s) 757
AvaI CYCGRG 2 cut(s) 22, 1047
AvaII GGWCC 1 cut(s) 1133
BaeGI GKGCMC 3 cut(s) 98, 670, 1352
BanI GGYRCC 3 cut(s) 95, 624, 695
BanII GRGCYC 4 cut(s) 329, 603, 916, 1048
BbsI GAAGAC 1 cut(s) 933
Bbv12I GWGCWC 3 cut(s) 329, 1039, 1352
BbvI GCAGC 4 cut(s) 622, 802, 1268, 1351
BccI CCATC 6 cut(s) 201, 377, 535, 563, 700, 736
BceAI ACGGC 1 cut(s) 1114
BcgI CGANNNNNNTGC 4 cut(s) 677, 711, 769, 803
BciT130I CCWGG 5 cut(s) 200, 296, 798, 1011, 1277
BclI TGATCA 2 cut(s) 60, 612
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 2 cut(s) 825, 921
BfmI CTRYAG 2 cut(s) 147, 636
BfuAI ACCTGC 1 cut(s) 683
BisI GCNGC 6 cut(s) 6, 636, 791, 1257, 1314, 1365
BlsI GCNGC 6 cut(s) 7, 637, 792, 1258, 1315, 1366
BmcAI AGTACT 1 cut(s) 1244
Bme1390I CCNGG 5 cut(s) 200, 296, 798, 1011, 1277
Bme18I GGWCC 1 cut(s) 1133
BmeRI GACNNNNNGTC 1 cut(s) 970
BmeT110I CYCGRG 2 cut(s) 22, 1047
BmgT120I GGNCC 2 cut(s) 1133, 1315
BmiI GGNNCC 8 cut(s) 34, 73, 97, 626, 697, 715, 915, 1135
BmrFI CCNGG 5 cut(s) 200, 296, 798, 1011, 1277
BmrI ACTGGG 1 cut(s) 919
BmsI GCATC 4 cut(s) 152, 679, 1243, 1258
BmuI ACTGGG 1 cut(s) 919
BpiI GAAGAC 1 cut(s) 933
BpmI CTGGAG 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 103
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 4 cut(s) 294, 295, 1009, 1261
BsaWI WCCGGW 4 cut(s) 42, 74, 347, 930
BsaXI ACNNNNNCTCC 4 cut(s) 842, 872, 1265, 1295
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 7 cut(s) 50, 99, 517, 850, 914, 1236, 1334
Bse3DI GCAATG 1 cut(s) 765
BseAI TCCGGA 1 cut(s) 74
BseBI CCWGG 5 cut(s) 200, 296, 798, 1011, 1277
BseDI CCNNGG 4 cut(s) 294, 295, 1009, 1261
BseGI GGATG 2 cut(s) 388, 711
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMI GCAATG 1 cut(s) 765
BseMII CTCAG 1 cut(s) 1031
BseNI ACTGG 7 cut(s) 50, 99, 517, 850, 914, 1236, 1334
BseRI GAGGAG 1 cut(s) 1308
BseSI GKGCMC 3 cut(s) 98, 670, 1352
BseXI GCAGC 4 cut(s) 622, 802, 1268, 1351
BsgI GTGCAG 1 cut(s) 809
Bsh1236I CGCG 1 cut(s) 605
Bsh1285I CGRYCG 1 cut(s) 892
BshFI GGCC 4 cut(s) 5, 86, 216, 1316
BshNI GGYRCC 3 cut(s) 95, 624, 695
BsiEI CGRYCG 1 cut(s) 892
BsiHKAI GWGCWC 3 cut(s) 329, 1039, 1352
BsiHKCI CYCGRG 2 cut(s) 22, 1047
BsiSI CCGG 6 cut(s) 43, 75, 348, 401, 699, 931
BslFI GGGAC 4 cut(s) 110, 815, 958, 1119
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 1 cut(s) 25
BsmFI GGGAC 4 cut(s) 110, 815, 958, 1119
BsmI GAATGC 1 cut(s) 754
BsnI GGCC 4 cut(s) 5, 86, 216, 1316
BsoBI CYCGRG 2 cut(s) 22, 1047
Bsp1286I GDGCHC 8 cut(s) 98, 329, 603, 670, 916, 1039, 1048, 1352
Bsp13I TCCGGA 1 cut(s) 74
Bsp1407I TGTACA 1 cut(s) 804
Bsp143I GATC 5 cut(s) 60, 612, 682, 933, 1354
BspACI CCGC 5 cut(s) 6, 170, 585, 603, 1313
BspANI GGCC 4 cut(s) 5, 86, 216, 1316
BspCNI CTCAG 1 cut(s) 1032
BspEI TCCGGA 1 cut(s) 74
BspFNI CGCG 1 cut(s) 605
BspHI TCATGA 4 cut(s) 321, 444, 558, 759
BspLI GGNNCC 8 cut(s) 34, 73, 97, 626, 697, 715, 915, 1135
BspMAI CTGCAG 2 cut(s) 151, 640
BspMI ACCTGC 1 cut(s) 683
BspPI GGATC 2 cut(s) 941, 1349
BspT107I GGYRCC 3 cut(s) 95, 624, 695
BsrDI GCAATG 1 cut(s) 765
BsrFI RCCGGY 1 cut(s) 698
BsrGI TGTACA 1 cut(s) 804
BsrI ACTGG 7 cut(s) 50, 99, 517, 850, 914, 1236, 1334
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 4 cut(s) 294, 295, 1009, 1261
BssMI GATC 5 cut(s) 60, 612, 682, 933, 1354
BssNI GRCGYC 1 cut(s) 47
Bst2UI CCWGG 5 cut(s) 200, 296, 798, 1011, 1277
Bst4CI ACNGT 6 cut(s) 19, 37, 629, 723, 730, 890
Bst6I CTCTTC 3 cut(s) 677, 810, 1214
BstACI GRCGYC 1 cut(s) 47
BstAUI TGTACA 1 cut(s) 804
BstC8I GCNNGC 3 cut(s) 151, 452, 603
BstDEI CTNAG 2 cut(s) 1040, 1155
BstF5I GGATG 2 cut(s) 388, 711
BstFNI CGCG 1 cut(s) 605
BstKTI GATC 5 cut(s) 63, 615, 685, 936, 1357
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 5 cut(s) 60, 612, 682, 933, 1354
BstMCI CGRYCG 1 cut(s) 892
BstMWI GCNNNNNNNGC 2 cut(s) 63, 1043
BstNI CCWGG 5 cut(s) 200, 296, 798, 1011, 1277
BstSCI CCNGG 5 cut(s) 198, 294, 796, 1009, 1275
BstSFI CTRYAG 2 cut(s) 147, 636
BstSLI GKGCMC 3 cut(s) 98, 670, 1352
BstUI CGCG 1 cut(s) 605
BstV1I GCAGC 4 cut(s) 622, 802, 1268, 1351
BstV2I GAAGAC 1 cut(s) 933
BstXI CCANNNNNNTGG 1 cut(s) 302
BsuRI GGCC 4 cut(s) 5, 86, 216, 1316
BtgZI GCGATG 1 cut(s) 379
BtsCI GGATG 2 cut(s) 388, 711
BtsIMutI CAGTG 5 cut(s) 57, 524, 726, 843, 1350
BveI ACCTGC 1 cut(s) 683
Cac8I GCNNGC 3 cut(s) 151, 452, 603
CciI TCATGA 4 cut(s) 321, 444, 558, 759
Cfr10I RCCGGY 1 cut(s) 698
Cfr13I GGNCC 2 cut(s) 1133, 1315
CseI GACGC 2 cut(s) 16, 55
Csp6I GTAC 6 cut(s) 625, 724, 805, 1243, 1331, 1411
CviAII CATG 8 cut(s) 10, 322, 379, 409, 445, 559, 760, 977
CviQI GTAC 6 cut(s) 625, 724, 805, 1243, 1331, 1411
DdeI CTNAG 2 cut(s) 1040, 1155
DpnI GATC 5 cut(s) 62, 614, 684, 935, 1356
DpnII GATC 5 cut(s) 60, 612, 682, 933, 1354
DriI GACNNNNNGTC 1 cut(s) 970
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 677, 810, 1214
Eam1105I GACNNNNNGTC 1 cut(s) 970
EarI CTCTTC 3 cut(s) 677, 810, 1214
Ecl136II GAGCTC 1 cut(s) 327
Eco147I AGGCCT 1 cut(s) 216
Eco24I GRGCYC 4 cut(s) 329, 603, 916, 1048
Eco47I GGWCC 1 cut(s) 1133
Eco53kI GAGCTC 1 cut(s) 327
Eco88I CYCGRG 2 cut(s) 22, 1047
EcoICRI GAGCTC 1 cut(s) 327
EcoO109I RGGNCCY 1 cut(s) 1133
EcoRI GAATTC 1 cut(s) 1298
EcoRII CCWGG 5 cut(s) 198, 294, 796, 1009, 1275
EcoT38I GRGCYC 4 cut(s) 329, 603, 916, 1048
FaeI CATG 8 cut(s) 13, 325, 382, 412, 448, 562, 763, 980
FaqI GGGAC 4 cut(s) 110, 815, 958, 1119
FatI CATG 8 cut(s) 9, 321, 378, 408, 444, 558, 759, 976
FauI CCCGC 2 cut(s) 578, 610
FbaI TGATCA 2 cut(s) 60, 612
Fnu4HI GCNGC 6 cut(s) 6, 636, 791, 1257, 1314, 1365
FokI GGATG 2 cut(s) 395, 718
FriOI GRGCYC 4 cut(s) 329, 603, 916, 1048
Fsp4HI GCNGC 6 cut(s) 6, 636, 791, 1257, 1314, 1365
FspBI CTAG 2 cut(s) 825, 921
GluI GCNGC 6 cut(s) 6, 636, 791, 1257, 1314, 1365
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 4 cut(s) 5, 86, 216, 1316
HapII CCGG 6 cut(s) 43, 75, 348, 401, 699, 931
HgaI GACGC 2 cut(s) 16, 55
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 8 cut(s) 13, 325, 382, 412, 448, 562, 763, 980
HincII GTYRAC 2 cut(s) 40, 1000
HindII GTYRAC 2 cut(s) 40, 1000
HindIII AAGCTT 2 cut(s) 416, 1112
HinfI GANTC 8 cut(s) 123, 275, 466, 820, 828, 951, 1224, 1280
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 6 cut(s) 43, 75, 348, 401, 699, 931
HphI GGTGA 1 cut(s) 757
Hpy166II GTNNAC 8 cut(s) 40, 427, 726, 733, 1000, 1054, 1120, 1350
Hpy188I TCNGA 5 cut(s) 32, 235, 1041, 1252, 1265
Hpy8I GTNNAC 8 cut(s) 40, 427, 726, 733, 1000, 1054, 1120, 1350
Hpy99I CGWCG 2 cut(s) 32, 182
HpyAV CCTTC 5 cut(s) 97, 130, 523, 1124, 1337
HpyCH4III ACNGT 6 cut(s) 19, 37, 629, 723, 730, 890
HpyCH4IV ACGT 1 cut(s) 786
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 1043
HpyF3I CTNAG 2 cut(s) 1040, 1155
HpySE526I ACGT 1 cut(s) 786
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 8 cut(s) 13, 325, 382, 412, 448, 562, 763, 980
Kpn2I TCCGGA 1 cut(s) 74
KpnI GGTACC 1 cut(s) 628
Ksp22I TGATCA 2 cut(s) 60, 612
KspAI GTTAAC 1 cut(s) 40
Kzo9I GATC 5 cut(s) 60, 612, 682, 933, 1354
LmnI GCTCC 5 cut(s) 295, 403, 919, 1179, 1204
Lsp1109I GCAGC 4 cut(s) 622, 802, 1268, 1351
LweI GCATC 4 cut(s) 152, 679, 1243, 1258
MaeI CTAG 2 cut(s) 825, 921
MaeII ACGT 1 cut(s) 786
MaeIII GTNAC 5 cut(s) 112, 841, 848, 884, 1391
MalI GATC 5 cut(s) 62, 614, 684, 935, 1356
MboI GATC 5 cut(s) 60, 612, 682, 933, 1354
MboII GAAGA 9 cut(s) 118, 664, 827, 938, 946, 992, 1021, 1120, 1231
MhlI GDGCHC 8 cut(s) 98, 329, 603, 670, 916, 1039, 1048, 1352
MluCI AATT 4 cut(s) 162, 546, 575, 1298
MlyI GAGTC 4 cut(s) 132, 837, 1233, 1274
MmeI TCCRAC 3 cut(s) 10, 1252, 1382
MroI TCCGGA 1 cut(s) 74
MseI TTAA 7 cut(s) 39, 158, 165, 462, 1179, 1287, 1326
MslI CAYNNNNRTG 2 cut(s) 560, 981
MspA1I CMGCKG 1 cut(s) 1364
MspI CCGG 6 cut(s) 43, 75, 348, 401, 699, 931
MspR9I CCNGG 5 cut(s) 200, 296, 798, 1011, 1277
Mva1269I GAATGC 1 cut(s) 754
MvaI CCWGG 5 cut(s) 200, 296, 798, 1011, 1277
MvnI CGCG 1 cut(s) 605
MwoI GCNNNNNNNGC 2 cut(s) 63, 1043
NdeII GATC 5 cut(s) 60, 612, 682, 933, 1354
NlaIII CATG 8 cut(s) 13, 325, 382, 412, 448, 562, 763, 980
NlaIV GGNNCC 8 cut(s) 34, 73, 97, 626, 697, 715, 915, 1135
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 4 cut(s) 112, 841, 884, 1391
PaeR7I CTCGAG 2 cut(s) 22, 1047
PagI TCATGA 4 cut(s) 321, 444, 558, 759
PaqCI CACCTGC 1 cut(s) 683
PasI CCCWGGG 1 cut(s) 295
PceI AGGCCT 1 cut(s) 216
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 889
PctI GAATGC 1 cut(s) 754
PfeI GAWTC 4 cut(s) 275, 466, 820, 951
PkrI GCNGC 6 cut(s) 7, 637, 792, 1258, 1315, 1366
PleI GAGTC 4 cut(s) 131, 836, 1232, 1274
PpsI GAGTC 4 cut(s) 131, 836, 1232, 1274
PpuMI RGGWCCY 1 cut(s) 1133
PshBI ATTAAT 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 329
Psp5II RGGWCCY 1 cut(s) 1133
Psp6I CCWGG 5 cut(s) 198, 294, 796, 1009, 1275
PspGI CCWGG 5 cut(s) 198, 294, 796, 1009, 1275
PspN4I GGNNCC 8 cut(s) 34, 73, 97, 626, 697, 715, 915, 1135
PspPI GGNCC 2 cut(s) 1133, 1315
PspPPI RGGWCCY 1 cut(s) 1133
PspXI VCTCGAGB 1 cut(s) 1047
PstI CTGCAG 2 cut(s) 151, 640
PvuII CAGCTG 1 cut(s) 1364
RsaI GTAC 6 cut(s) 626, 725, 806, 1244, 1332, 1412
RsaNI GTAC 6 cut(s) 625, 724, 805, 1243, 1331, 1411
RseI CAYNNNNRTG 2 cut(s) 560, 981
SacI GAGCTC 1 cut(s) 329
SaqAI TTAA 7 cut(s) 39, 158, 165, 462, 1179, 1287, 1326
SatI GCNGC 6 cut(s) 6, 636, 791, 1257, 1314, 1365
Sau3AI GATC 5 cut(s) 60, 612, 682, 933, 1354
Sau96I GGNCC 2 cut(s) 1133, 1315
ScaI AGTACT 1 cut(s) 1244
SchI GAGTC 4 cut(s) 132, 837, 1233, 1274
ScrFI CCNGG 5 cut(s) 200, 296, 798, 1011, 1277
SduI GDGCHC 8 cut(s) 98, 329, 603, 670, 916, 1039, 1048, 1352
SfaNI GCATC 4 cut(s) 152, 679, 1243, 1258
SfcI CTRYAG 2 cut(s) 147, 636
Sfr274I CTCGAG 2 cut(s) 22, 1047
SinI GGWCC 1 cut(s) 1133
SlaI CTCGAG 2 cut(s) 22, 1047
SmiMI CAYNNNNRTG 2 cut(s) 560, 981
SmlI CTYRAG 3 cut(s) 22, 118, 1047
SmoI CTYRAG 3 cut(s) 22, 118, 1047
Sse9I AATT 4 cut(s) 162, 546, 575, 1298
SseBI AGGCCT 1 cut(s) 216
SsiI CCGC 5 cut(s) 6, 170, 585, 603, 1313
SspMI CTAG 2 cut(s) 825, 921
SstI GAGCTC 1 cut(s) 329
StuI AGGCCT 1 cut(s) 216
StyD4I CCNGG 5 cut(s) 198, 294, 796, 1009, 1275
TaaI ACNGT 6 cut(s) 19, 37, 629, 723, 730, 890
TaiI ACGT 1 cut(s) 789
TaqI TCGA 7 cut(s) 23, 177, 355, 687, 1048, 1124, 1146
TasI AATT 4 cut(s) 162, 546, 575, 1298
TatI WGTACW 3 cut(s) 723, 804, 1242
TauI GCSGC 2 cut(s) 8, 1316
TfiI GAWTC 4 cut(s) 275, 466, 820, 951
Tru1I TTAA 7 cut(s) 39, 158, 165, 462, 1179, 1287, 1326
Tru9I TTAA 7 cut(s) 39, 158, 165, 462, 1179, 1287, 1326
TscAI CASTG 5 cut(s) 57, 524, 733, 850, 1357
TseFI GTSAC 4 cut(s) 112, 841, 884, 1391
TseI GCWGC 4 cut(s) 635, 790, 1256, 1364
Tsp45I GTSAC 4 cut(s) 112, 841, 884, 1391
TspDTI ATGAA 6 cut(s) 397, 433, 664, 776, 993, 1077
TspRI CASTG 5 cut(s) 57, 524, 733, 850, 1357
VneI GTGCAC 1 cut(s) 1348
VpaK11BI GGWCC 1 cut(s) 1133
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 1 cut(s) 1298
XhoI CTCGAG 2 cut(s) 22, 1047
XspI CTAG 2 cut(s) 825, 921
ZrmI AGTACT 1 cut(s) 1244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.