Prupe.2G238800_v2.0.a1

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000911
Physical Location & Seq
Reverse (-)
25930335 .. 25933376
3042 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G238800.2

Sequence Viewer

Length: 1425 bp
ATGGCTACTGCTGTCTCAACCATTGGAGCTGTCAACAGGGCACCGCTGAGCCTAAATGGCTCTAGCAGCAGCGCTTCAGTTCCAAGCTCAACCTTCTTGGGCAGCAGCTTGAAGAAAGTGAACTCAAGATTCACCAACTCCAAGGTTTCATCTGGGAGCTTCAGGATTGTAGCAGAGGTGGACGAAGATAAGCAGACCGACAAGGACAAATGGAGAGGCCTTGCCTATGACACCTCAGATGACCAACAAGACATCACAAGAGGAAAGGGCATGGTCGACTCTCTCTTCCAAGCACCCCAAGATGCTGGAACTCACTTCGCCGTCATGAGTTCTTATGAATACATCAGTACCGGGCTTCGCCAGTACAACTTGGACAACAACATGGATGGGTTTTACATTGCTCCTGCTTTTATGGACAAGCTTGTTGTCCACATCACCAAGAACTTCATGACCCTGCCTAACATCAAAGTTCCTCTCATCCTTGGTATCTGGGGAGGCAAAGGTCAGGGTAAATCTTTCCAGTGCGAGCTTGTCTTTGCCAAGATGGGAATCAGCCCCATCATGATGAGTGCCGGAGAATTGGAAAGTGGAAACGCAGGAGAACCCGCGAAGCTGATCAGGCAAAGGTACCGTGAAGCAGCTGACATAATCAGGAAGGGTAAAATGTGCGCACTCTTCATCAACGATCTTGATGCAGGAGCTGGTCGGCTTGGTGGAACCACACAGTACACTGTGAACAACCAAATGGTGAATGCCACCCTCATGAACATTGCTGACAACCCAACAAATGTCCAGCTCCCCGGTATGTACAACAAGGAGGAGAACCCCCGTGTCCCCATCATTGTCACCGGTAACGATTTCTCAACATTGTATGCTCCTCTCATCCGTGACGGGCGTATGGAGAAGTTCTACTGGGCGCCTACCCGCGAAGATCGTATTGGTGTCTGCATTGGGATCTTCAGGAGTGACAATGTTGCTAAGGAAGACATTGTCAAGCTTGTTGACACCTTCCCTGGCCAATCCATTGATTTCTTTGGTGCATTGAGGGCCCGAGTCTATGACGATGAAGTGAGGAAGTGGATTACAGGGGTTGGGGTTGACGGCGTTGGGAAGAGGCTTGTGAACTCAAAGGAAGGGCCCCCGACTTTCGAGCAGCCTAAGATGACAATCGAGAAGCTGCTTGAGTATGGAAACATGCTTGTCCAAGAGCAAGAGAATGTGAAGAGAGTCCAATTGGCTGATAAGTACTTGAGCGAGGCTGCTCTTGGTGATGCCAACTCAGATGCTATTGATAGTGGAACTTTCTACGGCAAAGCAGCCCAACAAGTTAACGTTCCAGTTCCTGAAGGTTGTACTGATCGAACTGCAGAAAACTTTGACCCAACTGCTAGGAGTGACGATGGCAGCTGCCTGTACACATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

475

Amino Acids

51.94

Weight (kDa)

5.34

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 670
Acc65I GGTACC 1 cut(s) 627
AccB1I GGYRCC 3 cut(s) 40, 627, 916
AccI GTMKAC 1 cut(s) 276
AccII CGCG 2 cut(s) 608, 927
AciI CCGC 3 cut(s) 44, 606, 925
AclI AACGTT 1 cut(s) 1332
AclWI GGATC 1 cut(s) 962
AcoI YGGCCR 1 cut(s) 1015
AcuI CTGAAG 4 cut(s) 60, 145, 943, 1365
AcyI GRCGYC 1 cut(s) 917
AfaI GTAC 8 cut(s) 349, 365, 629, 728, 809, 1247, 1354, 1415
AfeI AGCGCT 1 cut(s) 73
AgeI ACCGGT 1 cut(s) 848
AgsI TTSAA 1 cut(s) 112
AjnI CCWGG 1 cut(s) 1012
AjuI GAANNNNNNNTTGG 2 cut(s) 921, 953
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 1 cut(s) 962
AlwNI CAGNNNCTG 2 cut(s) 701, 1343
Ama87I CYCGRG 1 cut(s) 1050
Aor51HI AGCGCT 1 cut(s) 73
AoxI GGCC 4 cut(s) 217, 1015, 1047, 1136
ApaI GGGCCC 2 cut(s) 1051, 1140
AsiGI ACCGGT 1 cut(s) 848
Asp718I GGTACC 1 cut(s) 627
AspLEI GCGC 3 cut(s) 74, 671, 919
AspS9I GGNCC 4 cut(s) 1047, 1048, 1136, 1137
AsuC2I CCSGG 2 cut(s) 352, 801
AsuHPI GGTGA 5 cut(s) 124, 427, 760, 838, 1280
AvaI CYCGRG 1 cut(s) 1050
BaeGI GKGCMC 3 cut(s) 43, 1051, 1140
BaeI ACNNNNGTAYC 2 cut(s) 331, 364
BalI TGGCCA 1 cut(s) 1017
BanI GGYRCC 3 cut(s) 40, 627, 916
BanII GRGCYC 2 cut(s) 1051, 1140
BbsI GAAGAC 1 cut(s) 990
BccI CCATC 5 cut(s) 380, 538, 566, 845, 1394
BceAI ACGGC 3 cut(s) 305, 1117, 1324
BcgI CGANNNNNNTGC 2 cut(s) 674, 708
BciT130I CCWGG 1 cut(s) 1014
BclI TGATCA 1 cut(s) 615
BcnI CCSGG 2 cut(s) 352, 801
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 2 cut(s) 63, 1389
BfmI CTRYAG 1 cut(s) 1365
BfoI RGCGCY 2 cut(s) 75, 920
BglI GCCNNNNNGGC 1 cut(s) 57
BlpI GCTNAGC 1 cut(s) 47
BmcAI AGTACT 1 cut(s) 1247
Bme1390I CCNGG 3 cut(s) 352, 801, 1014
BmeT110I CYCGRG 1 cut(s) 1050
BmgT120I GGNCC 4 cut(s) 1047, 1048, 1136, 1137
BmiI GGNNCC 7 cut(s) 42, 629, 718, 918, 1049, 1138, 1139
BmrFI CCNGG 3 cut(s) 352, 801, 1014
BmrI ACTGGG 1 cut(s) 922
BmsI GCATC 4 cut(s) 292, 682, 1261, 1273
BmuI ACTGGG 1 cut(s) 922
BpiI GAAGAC 1 cut(s) 990
Bpu10I CCTNAGC 1 cut(s) 978
Bpu1102I GCTNAGC 1 cut(s) 47
BpuEI CTTGAG 3 cut(s) 109, 1202, 1270
BpuMI CCSGG 2 cut(s) 352, 801
BsaBI GATNNNNATC 2 cut(s) 548, 1166
BsaHI GRCGYC 1 cut(s) 917
BsaJI CCNNGG 4 cut(s) 141, 481, 799, 1012
BsaWI WCCGGW 1 cut(s) 848
Bse118I RCCGGY 1 cut(s) 848
Bse1I ACTGG 4 cut(s) 361, 520, 917, 1337
Bse3DI GCAATG 2 cut(s) 396, 768
Bse8I GATNNNNATC 2 cut(s) 548, 1166
BseBI CCWGG 1 cut(s) 1014
BseDI CCNNGG 4 cut(s) 141, 481, 799, 1012
BseGI GGATG 3 cut(s) 391, 477, 882
BseJI GATNNNNATC 2 cut(s) 548, 1166
BseMI GCAATG 2 cut(s) 396, 768
BseMII CTCAG 3 cut(s) 38, 249, 1293
BseNI ACTGG 4 cut(s) 361, 520, 917, 1337
BseRI GAGGAG 2 cut(s) 833, 867
BseSI GKGCMC 3 cut(s) 43, 1051, 1140
Bsh1236I CGCG 2 cut(s) 608, 927
BshFI GGCC 4 cut(s) 219, 1017, 1049, 1138
BshNI GGYRCC 3 cut(s) 40, 627, 916
BshTI ACCGGT 1 cut(s) 848
BsiHKCI CYCGRG 1 cut(s) 1050
BsiSI CCGG 4 cut(s) 351, 573, 801, 849
BslFI GGGAC 1 cut(s) 818
BsmAI GTCTC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 818
BsmI GAATGC 1 cut(s) 757
BsnI GGCC 4 cut(s) 219, 1017, 1049, 1138
BsoBI CYCGRG 1 cut(s) 1050
Bsp120I GGGCCC 2 cut(s) 1047, 1136
Bsp1286I GDGCHC 3 cut(s) 43, 1051, 1140
Bsp1407I TGTACA 2 cut(s) 807, 1413
Bsp143I GATC 5 cut(s) 615, 685, 931, 954, 1357
Bsp1720I GCTNAGC 1 cut(s) 47
BspACI CCGC 3 cut(s) 44, 606, 925
BspANI GGCC 4 cut(s) 219, 1017, 1049, 1138
BspCNI CTCAG 3 cut(s) 39, 248, 1292
BspFNI CGCG 2 cut(s) 608, 927
BspHI TCATGA 4 cut(s) 324, 447, 561, 762
BspLI GGNNCC 7 cut(s) 42, 629, 718, 918, 1049, 1138, 1139
BspMAI CTGCAG 1 cut(s) 1369
BspPI GGATC 1 cut(s) 962
BspT107I GGYRCC 3 cut(s) 40, 627, 916
BsrDI GCAATG 2 cut(s) 396, 768
BsrFI RCCGGY 1 cut(s) 848
BsrGI TGTACA 2 cut(s) 807, 1413
BsrI ACTGG 4 cut(s) 361, 520, 917, 1337
BssAI RCCGGY 1 cut(s) 848
BssECI CCNNGG 4 cut(s) 141, 481, 799, 1012
BssMI GATC 5 cut(s) 615, 685, 931, 954, 1357
BssNI GRCGYC 1 cut(s) 917
BssT1I CCWWGG 2 cut(s) 141, 481
Bst2UI CCWGG 1 cut(s) 1014
Bst4CI ACNGT 3 cut(s) 632, 726, 733
Bst6I CTCTTC 4 cut(s) 290, 680, 1106, 1217
BstACI GRCGYC 1 cut(s) 917
BstAUI TGTACA 2 cut(s) 807, 1413
BstC8I GCNNGC 1 cut(s) 527
BstDEI CTNAG 5 cut(s) 47, 235, 978, 1158, 1279
BstF5I GGATG 3 cut(s) 391, 477, 882
BstFNI CGCG 2 cut(s) 608, 927
BstH2I RGCGCY 2 cut(s) 75, 920
BstHHI GCGC 3 cut(s) 74, 671, 919
BstKTI GATC 5 cut(s) 618, 688, 934, 957, 1360
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 5 cut(s) 615, 685, 931, 954, 1357
BstMWI GCNNNNNNNGC 4 cut(s) 57, 66, 619, 1046
BstNI CCWGG 1 cut(s) 1014
BstNSI RCATGY 1 cut(s) 1198
BstSCI CCNGG 3 cut(s) 350, 799, 1012
BstSFI CTRYAG 1 cut(s) 1365
BstSLI GKGCMC 3 cut(s) 43, 1051, 1140
BstUI CGCG 2 cut(s) 608, 927
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 1 cut(s) 954
BstXI CCANNNNNNTGG 1 cut(s) 305
BstYI RGATCY 1 cut(s) 954
BsuRI GGCC 4 cut(s) 219, 1017, 1049, 1138
BtsCI GGATG 3 cut(s) 391, 477, 882
BtsIMutI CAGTG 2 cut(s) 527, 729
Cac8I GCNNGC 1 cut(s) 527
CaiI CAGNNNCTG 2 cut(s) 701, 1343
CciI TCATGA 4 cut(s) 324, 447, 561, 762
CfoI GCGC 3 cut(s) 74, 671, 919
Cfr10I RCCGGY 1 cut(s) 848
Cfr13I GGNCC 4 cut(s) 1047, 1048, 1136, 1137
Csp6I GTAC 8 cut(s) 348, 364, 628, 727, 808, 1246, 1353, 1414
CspAI ACCGGT 1 cut(s) 848
CviAII CATG 7 cut(s) 271, 325, 382, 448, 562, 763, 1195
CviQI GTAC 8 cut(s) 348, 364, 628, 727, 808, 1246, 1353, 1414
DdeI CTNAG 5 cut(s) 47, 235, 978, 1158, 1279
DinI GGCGCC 1 cut(s) 918
DpnI GATC 5 cut(s) 617, 687, 933, 956, 1359
DpnII GATC 5 cut(s) 615, 685, 931, 954, 1357
EaeI YGGCCR 1 cut(s) 1015
Eam1104I CTCTTC 4 cut(s) 290, 680, 1106, 1217
EarI CTCTTC 4 cut(s) 290, 680, 1106, 1217
Eco130I CCWWGG 2 cut(s) 141, 481
Eco147I AGGCCT 1 cut(s) 219
Eco24I GRGCYC 2 cut(s) 1051, 1140
Eco47III AGCGCT 1 cut(s) 73
Eco57I CTGAAG 4 cut(s) 60, 145, 943, 1365
Eco88I CYCGRG 1 cut(s) 1050
EcoO109I RGGNCCY 3 cut(s) 1047, 1136, 1137
EcoRII CCWGG 1 cut(s) 1012
EcoT14I CCWWGG 2 cut(s) 141, 481
EcoT38I GRGCYC 2 cut(s) 1051, 1140
EgeI GGCGCC 1 cut(s) 918
EheI GGCGCC 1 cut(s) 918
ErhI CCWWGG 2 cut(s) 141, 481
FaeI CATG 7 cut(s) 274, 328, 385, 451, 565, 766, 1198
FaqI GGGAC 1 cut(s) 818
FatI CATG 7 cut(s) 270, 324, 381, 447, 561, 762, 1194
FauI CCCGC 2 cut(s) 613, 932
FbaI TGATCA 1 cut(s) 615
FblI GTMKAC 1 cut(s) 276
FokI GGATG 3 cut(s) 398, 464, 869
FriOI GRGCYC 2 cut(s) 1051, 1140
FspAI RTGCGCAY 1 cut(s) 670
FspBI CTAG 2 cut(s) 63, 1389
FspI TGCGCA 1 cut(s) 670
GlaI GCGC 3 cut(s) 73, 670, 918
HaeII RGCGCY 2 cut(s) 75, 920
HaeIII GGCC 4 cut(s) 219, 1017, 1049, 1138
HapII CCGG 4 cut(s) 351, 573, 801, 849
HhaI GCGC 3 cut(s) 74, 671, 919
Hin1I GRCGYC 1 cut(s) 917
Hin1II CATG 7 cut(s) 274, 328, 385, 451, 565, 766, 1198
Hin6I GCGC 3 cut(s) 72, 669, 917
HinP1I GCGC 3 cut(s) 72, 669, 917
HincII GTYRAC 5 cut(s) 34, 277, 1003, 1099, 1330
HindII GTYRAC 5 cut(s) 34, 277, 1003, 1099, 1330
HindIII AAGCTT 2 cut(s) 419, 995
HinfI GANTC 5 cut(s) 129, 278, 549, 1053, 1227
HpaI GTTAAC 1 cut(s) 1330
HpaII CCGG 4 cut(s) 351, 573, 801, 849
HphI GGTGA 5 cut(s) 124, 427, 760, 838, 1280
Hpy188I TCNGA 2 cut(s) 238, 1282
HpyAV CCTTC 5 cut(s) 103, 649, 1018, 1127, 1340
HpyCH4III ACNGT 3 cut(s) 632, 726, 733
HpyCH4IV ACGT 1 cut(s) 1332
HpyCH4V TGCA 4 cut(s) 695, 948, 1040, 1367
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 66, 619, 1046
HpyF3I CTNAG 5 cut(s) 47, 235, 978, 1158, 1279
HpySE526I ACGT 1 cut(s) 1332
Hsp92I GRCGYC 1 cut(s) 917
Hsp92II CATG 7 cut(s) 274, 328, 385, 451, 565, 766, 1198
HspAI GCGC 3 cut(s) 72, 669, 917
KasI GGCGCC 1 cut(s) 916
KpnI GGTACC 1 cut(s) 631
Ksp22I TGATCA 1 cut(s) 615
KspAI GTTAAC 1 cut(s) 1330
Kzo9I GATC 5 cut(s) 615, 685, 931, 954, 1357
LmnI GCTCC 6 cut(s) 26, 156, 406, 698, 801, 880
LweI GCATC 4 cut(s) 292, 682, 1261, 1273
MaeI CTAG 2 cut(s) 63, 1389
MaeII ACGT 1 cut(s) 1332
MaeIII GTNAC 5 cut(s) 844, 851, 887, 965, 1394
MalI GATC 5 cut(s) 617, 687, 933, 956, 1359
MboI GATC 5 cut(s) 615, 685, 931, 954, 1357
MboII GAAGA 9 cut(s) 124, 197, 277, 667, 941, 949, 995, 1123, 1234
MfeI CAATTG 1 cut(s) 1232
MflI RGATCY 1 cut(s) 954
MhlI GDGCHC 3 cut(s) 43, 1051, 1140
MlsI TGGCCA 1 cut(s) 1017
MluCI AATT 2 cut(s) 578, 1232
MluNI TGGCCA 1 cut(s) 1017
Mly113I GGCGCC 1 cut(s) 917
MlyI GAGTC 3 cut(s) 272, 1062, 1236
Mox20I TGGCCA 1 cut(s) 1017
MscI TGGCCA 1 cut(s) 1017
MseI TTAA 2 cut(s) 1329, 1423
MslI CAYNNNNRTG 2 cut(s) 563, 761
Msp20I TGGCCA 1 cut(s) 1017
MspA1I CMGCKG 3 cut(s) 46, 641, 1407
MspI CCGG 4 cut(s) 351, 573, 801, 849
MspR9I CCNGG 3 cut(s) 352, 801, 1014
MunI CAATTG 1 cut(s) 1232
Mva1269I GAATGC 1 cut(s) 757
MvaI CCWGG 1 cut(s) 1014
MvnI CGCG 2 cut(s) 608, 927
MwoI GCNNNNNNNGC 4 cut(s) 57, 66, 619, 1046
NarI GGCGCC 1 cut(s) 917
NciI CCSGG 2 cut(s) 352, 801
NdeII GATC 5 cut(s) 615, 685, 931, 954, 1357
NlaIII CATG 7 cut(s) 274, 328, 385, 451, 565, 766, 1198
NlaIV GGNNCC 7 cut(s) 42, 629, 718, 918, 1049, 1138, 1139
NmuCI GTSAC 4 cut(s) 844, 887, 965, 1394
NsbI TGCGCA 1 cut(s) 670
NspI RCATGY 1 cut(s) 1198
PagI TCATGA 4 cut(s) 324, 447, 561, 762
PceI AGGCCT 1 cut(s) 219
PctI GAATGC 1 cut(s) 757
PfeI GAWTC 2 cut(s) 129, 549
PflFI GACNNNGTC 1 cut(s) 989
PinAI ACCGGT 1 cut(s) 848
PleI GAGTC 3 cut(s) 272, 1061, 1235
PluTI GGCGCC 1 cut(s) 920
PpsI GAGTC 3 cut(s) 272, 1061, 1235
Psp1406I AACGTT 1 cut(s) 1332
Psp6I CCWGG 1 cut(s) 1012
PspGI CCWGG 1 cut(s) 1012
PspN4I GGNNCC 7 cut(s) 42, 629, 718, 918, 1049, 1138, 1139
PspOMI GGGCCC 2 cut(s) 1047, 1136
PspPI GGNCC 4 cut(s) 1047, 1048, 1136, 1137
PstI CTGCAG 1 cut(s) 1369
PstNI CAGNNNCTG 2 cut(s) 701, 1343
PsuI RGATCY 1 cut(s) 954
PsyI GACNNNGTC 1 cut(s) 989
PvuII CAGCTG 2 cut(s) 641, 1407
RsaI GTAC 8 cut(s) 349, 365, 629, 728, 809, 1247, 1354, 1415
RsaNI GTAC 8 cut(s) 348, 364, 628, 727, 808, 1246, 1353, 1414
RseI CAYNNNNRTG 2 cut(s) 563, 761
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 2 cut(s) 1329, 1423
Sau3AI GATC 5 cut(s) 615, 685, 931, 954, 1357
Sau96I GGNCC 4 cut(s) 1047, 1048, 1136, 1137
ScaI AGTACT 1 cut(s) 1247
SchI GAGTC 3 cut(s) 272, 1062, 1236
ScrFI CCNGG 3 cut(s) 352, 801, 1014
SduI GDGCHC 3 cut(s) 43, 1051, 1140
SfaNI GCATC 4 cut(s) 292, 682, 1261, 1273
SfcI CTRYAG 1 cut(s) 1365
SfoI GGCGCC 1 cut(s) 918
SmiMI CAYNNNNRTG 2 cut(s) 563, 761
SmlI CTYRAG 3 cut(s) 124, 1181, 1249
SmoI CTYRAG 3 cut(s) 124, 1181, 1249
Sse9I AATT 2 cut(s) 578, 1232
SseBI AGGCCT 1 cut(s) 219
SsiI CCGC 3 cut(s) 44, 606, 925
SspDI GGCGCC 1 cut(s) 916
SspMI CTAG 2 cut(s) 63, 1389
StuI AGGCCT 1 cut(s) 219
StyD4I CCNGG 3 cut(s) 350, 799, 1012
StyI CCWWGG 2 cut(s) 141, 481
TaaI ACNGT 3 cut(s) 632, 726, 733
TaiI ACGT 1 cut(s) 1335
TaqI TCGA 4 cut(s) 276, 1149, 1170, 1360
TaqII GACCGA 1 cut(s) 212
TasI AATT 2 cut(s) 578, 1232
TatI WGTACW 6 cut(s) 363, 726, 807, 1245, 1352, 1413
TfiI GAWTC 2 cut(s) 129, 549
Tru1I TTAA 2 cut(s) 1329, 1423
Tru9I TTAA 2 cut(s) 1329, 1423
TscAI CASTG 2 cut(s) 527, 736
TseFI GTSAC 4 cut(s) 844, 887, 965, 1394
Tsp45I GTSAC 4 cut(s) 844, 887, 965, 1394
TspDTI ATGAA 6 cut(s) 138, 351, 436, 667, 779, 1080
TspGWI ACGGA 1 cut(s) 875
TspRI CASTG 2 cut(s) 527, 736
Tth111I GACNNNGTC 1 cut(s) 989
XceI RCATGY 1 cut(s) 1198
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 2 cut(s) 63, 1389
ZrmI AGTACT 1 cut(s) 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.