Rh5AG435700

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000911
Physical Location & Seq
Reverse (-)
74901123 .. 74904064
2942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG435700.1

Sequence Viewer

Length: 1392 bp
ATGCAGTTGAGCTTGAACAGCTCTGGTGCTGGAAGCTCAATCCCAAGCTCAGCTTTCTTGGGCAGCAGTTTGAAGAAGGTCAACAACACTAGACTTCCCTACCAAAAATCTGTATCTGCACGTAGCTTGAGAATCACAGCAGAAATCGATGAGGGAAAGCAGTCGAAATCAGACAAGTGGAAAGGCCTTGCTTATGACATTTCTGATGACCAACAAGACATTACAAGAGGAAAGGGAATGGTTGATTCTGTGTTCCAAGCTCCCACTGGGACTGGGACTCACAATGCCATCATGAGCTCATATGATTACATCAGCACCGGACTTCGCACGTACAACTTGGATAACAACATGGATGGGTTTTACATAGCTCCAGCTTTCATGGACAAGCTTGTGGTTCATATCACCAAGAACTTCATGAGCCTGCCTAACATTAAGGTTCCCCTTATTCTTGGTATATGGGGAGGAAAAGGTCAGGGAAAATCATTCCAGTGTGAGCTTGTGTTTGCCAAGATGGGAATTACCCCCATCATGATGAGTGCTGGAGAATTAGAAAGTGGGAATGCAGGAGAGCCAGCAAAGTTGATCAGACAAAGGTATCGGGAGGCTTCAGACATCATCCGAAAAGGAAAAATGTGTGCCCTCTTCATCAATGATCTCGATGCAGGTGCTGGTAGGATGGGTGGAACCACACAGTACACTGTGAACAACCAGATGGTCAACGCAACCCTCATGAACATTGCTGATAACCCAACAAATGTGCAGCTTCCCGGTATGTACAACAAGGAGGACAATGCTAGAGTGCCGGTTATAGTCACTGGTAATGACTTCTCTACACTCTATGCGCCTCTTATCCGTGATGGTCGTATGGAGAAGTTTTACTGGGCTCCCACAAGGGAAGACCGGATTGGTGTTTGCATCGGAATCTTCAAGACTGACAATATTCCTGAGGAGGCTGTTGTTAAGATTGTAGACACATTCCCTGGTCAATCTATTGATTTCTTTGGTGCCCTTCGAGCTCGAGTCTACGATGATGAAGTGAGGAAGTGGATATCCGGTGTTGGAGTAGACGGCATTGGGAAAAAGCTTGTGAACTCAAAAGAAGGTCTCCCAACTTTTGAGCAGCCTAAGATGACACTGGAGAAGCTTCTTGAGTATGGTAACATGCTTGTTCAAGAGCAGGACAATGTCAAGAGAGTTCAACTTGCTGACACTTACTTGAGTCAAGCAGCACTTGGAGATGCTAACCGGGATTCTATTAAAAGCGGAGATTTCTATGGCCAGGCAGCCCAGCAAGTTCATGTACCAGTTCCTGAAGGATGTACTGATCGAACAGCCTCAAACTTTGACCCAACAGCTAGGAGTGACGATGGTAGCTGTGAGTACCAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

463

Amino Acids

50.8

Weight (kDa)

5.84

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 152 - 295 7e-17 ATPase family associated with various cellular activities (AAA)
RuBisCO_activase_AAA_helical PF21228 298 - 393 5.3e-40 Ribulose bisphosphate carboxylase/oxygenase activase, AAA, helical
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 653
Acc36I ACCTGC 1 cut(s) 653
AccB1I GGYRCC 1 cut(s) 1004
AccI GTMKAC 3 cut(s) 969, 1023, 1065
AciI CCGC 1 cut(s) 1263
AcoI YGGCCR 1 cut(s) 1276
AcuI CTGAAG 2 cut(s) 591, 1332
AfaI GTAC 6 cut(s) 332, 695, 776, 1302, 1321, 1382
AgsI TTSAA 5 cut(s) 16, 73, 928, 1172, 1199
AjnI CCWGG 2 cut(s) 979, 1278
AjuI GAANNNNNNNTTGG 2 cut(s) 888, 920
Alw21I GWGCWC 2 cut(s) 299, 1018
Alw26I GTCTC 1 cut(s) 1109
AlwNI CAGNNNCTG 2 cut(s) 668, 1310
Ama87I CYCGRG 1 cut(s) 1017
AoxI GGCC 2 cut(s) 184, 1276
ApeKI GCWGC 5 cut(s) 63, 760, 1120, 1226, 1283
AspLEI GCGC 1 cut(s) 844
AsuC2I CCSGG 2 cut(s) 768, 1247
AsuHPI GGTGA 1 cut(s) 394
AvaI CYCGRG 1 cut(s) 1017
AxyI CCTNAGG 1 cut(s) 945
BaeGI GKGCMC 2 cut(s) 640, 1009
BaeI ACNNNNGTAYC 2 cut(s) 578, 611
BalI TGGCCA 1 cut(s) 1278
BanI GGYRCC 1 cut(s) 1004
BanII GRGCYC 3 cut(s) 299, 886, 1018
BbsI GAAGAC 1 cut(s) 903
Bbv12I GWGCWC 2 cut(s) 299, 1018
BbvI GCAGC 5 cut(s) 75, 772, 1132, 1238, 1295
BccI CCATC 8 cut(s) 296, 347, 505, 533, 670, 706, 851, 1361
BceAI ACGGC 1 cut(s) 1084
BcgI CGANNNNNNTGC 2 cut(s) 647, 681
BciT130I CCWGG 2 cut(s) 981, 1280
BclI TGATCA 1 cut(s) 582
BcnI CCSGG 2 cut(s) 768, 1247
BcoDI GTCTC 1 cut(s) 1109
BfaI CTAG 3 cut(s) 90, 795, 1356
BfuAI ACCTGC 1 cut(s) 653
BisI GCNGC 5 cut(s) 64, 761, 1121, 1227, 1284
BlpI GCTNAGC 1 cut(s) 49
BlsI GCNGC 5 cut(s) 65, 762, 1122, 1228, 1285
Bme1390I CCNGG 4 cut(s) 768, 981, 1247, 1280
BmeT110I CYCGRG 1 cut(s) 1017
BmiI GGNNCC 4 cut(s) 438, 685, 885, 1006
BmrFI CCNGG 4 cut(s) 768, 981, 1247, 1280
BmrI ACTGGG 3 cut(s) 276, 282, 889
BmsI GCATC 3 cut(s) 649, 924, 1228
BmuI ACTGGG 3 cut(s) 276, 282, 889
BpiI GAAGAC 1 cut(s) 903
BpmI CTGGAG 3 cut(s) 354, 561, 1157
Bpu1102I GCTNAGC 1 cut(s) 49
BpuEI CTTGAG 3 cut(s) 148, 1169, 1237
BpuMI CCSGG 2 cut(s) 768, 1247
Bsa29I ATCGAT 1 cut(s) 147
BsaAI YACGTR 2 cut(s) 122, 330
BsaI GGTCTC 1 cut(s) 1109
BsaJI CCNNGG 1 cut(s) 979
BsaWI WCCGGW 3 cut(s) 317, 900, 1052
Bse118I RCCGGY 1 cut(s) 802
Bse1I ACTGG 7 cut(s) 271, 277, 487, 820, 884, 1140, 1304
Bse21I CCTNAGG 1 cut(s) 945
Bse3DI GCAATG 1 cut(s) 735
BseBI CCWGG 2 cut(s) 981, 1280
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 979
BseGI GGATG 4 cut(s) 358, 615, 681, 1322
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 2 cut(s) 63, 936
BseNI ACTGG 7 cut(s) 271, 277, 487, 820, 884, 1140, 1304
BseRI GAGGAG 1 cut(s) 962
BseSI GKGCMC 2 cut(s) 640, 1009
BseXI GCAGC 5 cut(s) 75, 772, 1132, 1238, 1295
BseYI CCCAGC 1 cut(s) 1287
BsgI GTGCAG 2 cut(s) 102, 779
BshFI GGCC 2 cut(s) 186, 1278
BshNI GGYRCC 1 cut(s) 1004
BshVI ATCGAT 1 cut(s) 147
BsiHKAI GWGCWC 2 cut(s) 299, 1018
BsiHKCI CYCGRG 1 cut(s) 1017
BsiSI CCGG 6 cut(s) 318, 768, 803, 901, 1053, 1246
BslFI GGGAC 2 cut(s) 283, 289
BsmAI GTCTC 1 cut(s) 1109
BsmFI GGGAC 2 cut(s) 283, 289
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 2 cut(s) 186, 1278
Bso31I GGTCTC 1 cut(s) 1109
BsoBI CYCGRG 1 cut(s) 1017
Bsp1286I GDGCHC 5 cut(s) 299, 640, 886, 1009, 1018
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 3 cut(s) 582, 652, 1324
Bsp1720I GCTNAGC 1 cut(s) 49
BspACI CCGC 1 cut(s) 1263
BspANI GGCC 2 cut(s) 186, 1278
BspCNI CTCAG 2 cut(s) 62, 937
BspDI ATCGAT 1 cut(s) 147
BspHI TCATGA 4 cut(s) 291, 414, 528, 729
BspLI GGNNCC 4 cut(s) 438, 685, 885, 1006
BspMI ACCTGC 1 cut(s) 653
BspT107I GGYRCC 1 cut(s) 1004
BspTNI GGTCTC 1 cut(s) 1109
BsrDI GCAATG 1 cut(s) 735
BsrFI RCCGGY 1 cut(s) 802
BsrGI TGTACA 1 cut(s) 774
BsrI ACTGG 7 cut(s) 271, 277, 487, 820, 884, 1140, 1304
BssAI RCCGGY 1 cut(s) 802
BssECI CCNNGG 1 cut(s) 979
BssMI GATC 3 cut(s) 582, 652, 1324
Bst2UI CCWGG 2 cut(s) 981, 1280
Bst4CI ACNGT 2 cut(s) 693, 700
Bst6I CTCTTC 1 cut(s) 647
BstAUI TGTACA 1 cut(s) 774
BstBAI YACGTR 2 cut(s) 122, 330
BstC8I GCNNGC 2 cut(s) 422, 573
BstDEI CTNAG 3 cut(s) 49, 945, 1125
BstF5I GGATG 4 cut(s) 358, 615, 681, 1322
BstHHI GCGC 1 cut(s) 844
BstKTI GATC 3 cut(s) 585, 655, 1327
BstMAI GTCTC 1 cut(s) 1109
BstMBI GATC 3 cut(s) 582, 652, 1324
BstMWI GCNNNNNNNGC 2 cut(s) 18, 1013
BstNI CCWGG 2 cut(s) 981, 1280
BstNSI RCATGY 1 cut(s) 1165
BstSCI CCNGG 4 cut(s) 766, 979, 1245, 1278
BstSLI GKGCMC 2 cut(s) 640, 1009
BstV1I GCAGC 5 cut(s) 75, 772, 1132, 1238, 1295
BstV2I GAAGAC 1 cut(s) 903
Bsu15I ATCGAT 1 cut(s) 147
Bsu36I CCTNAGG 1 cut(s) 945
BsuRI GGCC 2 cut(s) 186, 1278
BsuTUI ATCGAT 1 cut(s) 147
BtsCI GGATG 4 cut(s) 358, 615, 681, 1322
BtsIMutI CAGTG 5 cut(s) 264, 494, 696, 813, 1133
BveI ACCTGC 1 cut(s) 653
Cac8I GCNNGC 2 cut(s) 422, 573
CaiI CAGNNNCTG 2 cut(s) 668, 1310
CciI TCATGA 4 cut(s) 291, 414, 528, 729
CfoI GCGC 1 cut(s) 844
Cfr10I RCCGGY 1 cut(s) 802
ClaI ATCGAT 1 cut(s) 147
Csp6I GTAC 6 cut(s) 331, 694, 775, 1301, 1320, 1381
CviAII CATG 8 cut(s) 292, 349, 379, 415, 529, 730, 1162, 1298
CviQI GTAC 6 cut(s) 331, 694, 775, 1301, 1320, 1381
DdeI CTNAG 3 cut(s) 49, 945, 1125
DpnI GATC 3 cut(s) 584, 654, 1326
DpnII GATC 3 cut(s) 582, 652, 1324
EaeI YGGCCR 1 cut(s) 1276
Eam1104I CTCTTC 1 cut(s) 647
EarI CTCTTC 1 cut(s) 647
Ecl136II GAGCTC 2 cut(s) 297, 1016
Eco147I AGGCCT 1 cut(s) 186
Eco24I GRGCYC 3 cut(s) 299, 886, 1018
Eco31I GGTCTC 1 cut(s) 1109
Eco32I GATATC 1 cut(s) 1050
Eco53kI GAGCTC 2 cut(s) 297, 1016
Eco57I CTGAAG 2 cut(s) 591, 1332
Eco81I CCTNAGG 1 cut(s) 945
Eco88I CYCGRG 1 cut(s) 1017
EcoICRI GAGCTC 2 cut(s) 297, 1016
EcoRII CCWGG 2 cut(s) 979, 1278
EcoRV GATATC 1 cut(s) 1050
EcoT38I GRGCYC 3 cut(s) 299, 886, 1018
FaeI CATG 8 cut(s) 295, 352, 382, 418, 532, 733, 1165, 1301
FalI AAGNNNNNCTT 4 cut(s) 37, 69, 1215, 1247
FaqI GGGAC 2 cut(s) 283, 289
FatI CATG 8 cut(s) 291, 348, 378, 414, 528, 729, 1161, 1297
FauNDI CATATG 1 cut(s) 301
FbaI TGATCA 1 cut(s) 582
FblI GTMKAC 3 cut(s) 969, 1023, 1065
Fnu4HI GCNGC 5 cut(s) 64, 761, 1121, 1227, 1284
FokI GGATG 4 cut(s) 365, 602, 688, 1329
FriOI GRGCYC 3 cut(s) 299, 886, 1018
Fsp4HI GCNGC 5 cut(s) 64, 761, 1121, 1227, 1284
FspBI CTAG 3 cut(s) 90, 795, 1356
GlaI GCGC 1 cut(s) 843
GluI GCNGC 5 cut(s) 64, 761, 1121, 1227, 1284
GsaI CCCAGC 1 cut(s) 1291
GsuI CTGGAG 3 cut(s) 354, 561, 1157
HaeIII GGCC 2 cut(s) 186, 1278
HapII CCGG 6 cut(s) 318, 768, 803, 901, 1053, 1246
HhaI GCGC 1 cut(s) 844
Hin1II CATG 8 cut(s) 295, 352, 382, 418, 532, 733, 1165, 1301
Hin6I GCGC 1 cut(s) 842
HinP1I GCGC 1 cut(s) 842
HincII GTYRAC 2 cut(s) 82, 718
HindII GTYRAC 2 cut(s) 82, 718
HindIII AAGCTT 3 cut(s) 386, 1082, 1142
HinfI GANTC 7 cut(s) 132, 245, 277, 921, 1020, 1219, 1250
HpaII CCGG 6 cut(s) 318, 768, 803, 901, 1053, 1246
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 8 cut(s) 82, 696, 703, 718, 970, 1024, 1066, 1090
Hpy188I TCNGA 6 cut(s) 172, 205, 587, 610, 620, 920
Hpy8I GTNNAC 8 cut(s) 82, 696, 703, 718, 970, 1024, 1066, 1090
HpyAV CCTTC 4 cut(s) 70, 1019, 1094, 1307
HpyCH4III ACNGT 2 cut(s) 693, 700
HpyCH4IV ACGT 2 cut(s) 121, 329
HpyCH4V TGCA 6 cut(s) 4, 119, 563, 662, 760, 915
HpyF10VI GCNNNNNNNGC 2 cut(s) 18, 1013
HpyF3I CTNAG 3 cut(s) 49, 945, 1125
HpySE526I ACGT 2 cut(s) 121, 329
Hsp92II CATG 8 cut(s) 295, 352, 382, 418, 532, 733, 1165, 1301
HspAI GCGC 1 cut(s) 842
Ksp22I TGATCA 1 cut(s) 582
Kzo9I GATC 3 cut(s) 582, 652, 1324
LmnI GCTCC 3 cut(s) 265, 373, 889
Lsp1109I GCAGC 5 cut(s) 75, 772, 1132, 1238, 1295
LweI GCATC 3 cut(s) 649, 924, 1228
MaeI CTAG 3 cut(s) 90, 795, 1356
MaeII ACGT 2 cut(s) 121, 329
MaeIII GTNAC 3 cut(s) 811, 1157, 1361
MalI GATC 3 cut(s) 584, 654, 1326
MboI GATC 3 cut(s) 582, 652, 1324
MboII GAAGA 4 cut(s) 85, 634, 908, 916
MhlI GDGCHC 5 cut(s) 299, 640, 886, 1009, 1018
MlsI TGGCCA 1 cut(s) 1278
MluCI AATT 2 cut(s) 516, 545
MluNI TGGCCA 1 cut(s) 1278
MlyI GAGTC 3 cut(s) 271, 1029, 1228
MmeI TCCRAC 1 cut(s) 1039
Mox20I TGGCCA 1 cut(s) 1278
MscI TGGCCA 1 cut(s) 1278
MseI TTAA 3 cut(s) 432, 960, 1257
MslI CAYNNNNRTG 2 cut(s) 487, 530
Msp20I TGGCCA 1 cut(s) 1278
MspI CCGG 6 cut(s) 318, 768, 803, 901, 1053, 1246
MspR9I CCNGG 4 cut(s) 768, 981, 1247, 1280
Mva1269I GAATGC 1 cut(s) 565
MvaI CCWGG 2 cut(s) 981, 1280
MwoI GCNNNNNNNGC 2 cut(s) 18, 1013
NciI CCSGG 2 cut(s) 768, 1247
NdeI CATATG 1 cut(s) 301
NdeII GATC 3 cut(s) 582, 652, 1324
NlaIII CATG 8 cut(s) 295, 352, 382, 418, 532, 733, 1165, 1301
NlaIV GGNNCC 4 cut(s) 438, 685, 885, 1006
NmuCI GTSAC 2 cut(s) 811, 1361
NspI RCATGY 1 cut(s) 1165
PaeR7I CTCGAG 1 cut(s) 1017
PagI TCATGA 4 cut(s) 291, 414, 528, 729
PaqCI CACCTGC 1 cut(s) 653
PceI AGGCCT 1 cut(s) 186
PctI GAATGC 1 cut(s) 565
PfeI GAWTC 4 cut(s) 132, 245, 921, 1250
PflFI GACNNNGTC 1 cut(s) 1184
PkrI GCNGC 5 cut(s) 65, 762, 1122, 1228, 1285
PleI GAGTC 3 cut(s) 271, 1028, 1227
PpsI GAGTC 3 cut(s) 271, 1028, 1227
Ppu21I YACGTR 2 cut(s) 122, 330
Psp124BI GAGCTC 2 cut(s) 299, 1018
Psp6I CCWGG 2 cut(s) 979, 1278
PspFI CCCAGC 1 cut(s) 1287
PspGI CCWGG 2 cut(s) 979, 1278
PspN4I GGNNCC 4 cut(s) 438, 685, 885, 1006
PspXI VCTCGAGB 1 cut(s) 1017
PstNI CAGNNNCTG 2 cut(s) 668, 1310
PsyI GACNNNGTC 1 cut(s) 1184
RsaI GTAC 6 cut(s) 332, 695, 776, 1302, 1321, 1382
RsaNI GTAC 6 cut(s) 331, 694, 775, 1301, 1320, 1381
RseI CAYNNNNRTG 2 cut(s) 487, 530
SacI GAGCTC 2 cut(s) 299, 1018
SaqAI TTAA 3 cut(s) 432, 960, 1257
SatI GCNGC 5 cut(s) 64, 761, 1121, 1227, 1284
Sau3AI GATC 3 cut(s) 582, 652, 1324
SchI GAGTC 3 cut(s) 271, 1029, 1228
ScrFI CCNGG 4 cut(s) 768, 981, 1247, 1280
SduI GDGCHC 5 cut(s) 299, 640, 886, 1009, 1018
SfaNI GCATC 3 cut(s) 649, 924, 1228
Sfr274I CTCGAG 1 cut(s) 1017
SlaI CTCGAG 1 cut(s) 1017
SmiMI CAYNNNNRTG 2 cut(s) 487, 530
SmlI CTYRAG 4 cut(s) 127, 1017, 1148, 1216
SmoI CTYRAG 4 cut(s) 127, 1017, 1148, 1216
Sse9I AATT 2 cut(s) 516, 545
SseBI AGGCCT 1 cut(s) 186
SsiI CCGC 1 cut(s) 1263
SspI AATATT 1 cut(s) 940
SspMI CTAG 3 cut(s) 90, 795, 1356
SstI GAGCTC 2 cut(s) 299, 1018
StuI AGGCCT 1 cut(s) 186
StyD4I CCNGG 4 cut(s) 766, 979, 1245, 1278
TaaI ACNGT 2 cut(s) 693, 700
TaiI ACGT 2 cut(s) 124, 332
TaqI TCGA 6 cut(s) 147, 164, 657, 1012, 1018, 1327
TasI AATT 2 cut(s) 516, 545
TatI WGTACW 3 cut(s) 693, 774, 1319
TfiI GAWTC 4 cut(s) 132, 245, 921, 1250
Tru1I TTAA 3 cut(s) 432, 960, 1257
Tru9I TTAA 3 cut(s) 432, 960, 1257
TscAI CASTG 5 cut(s) 271, 494, 703, 820, 1140
TseFI GTSAC 2 cut(s) 811, 1361
TseI GCWGC 5 cut(s) 63, 760, 1120, 1226, 1283
Tsp45I GTSAC 2 cut(s) 811, 1361
TspDTI ATGAA 7 cut(s) 367, 386, 403, 634, 746, 1047, 1286
TspGWI ACGGA 1 cut(s) 842
TspRI CASTG 5 cut(s) 271, 494, 703, 820, 1140
Tth111I GACNNNGTC 1 cut(s) 1184
XceI RCATGY 1 cut(s) 1165
XcmI CCANNNNNNNNNTGG 1 cut(s) 263
XhoI CTCGAG 1 cut(s) 1017
XmiI GTMKAC 3 cut(s) 969, 1023, 1065
XspI CTAG 3 cut(s) 90, 795, 1356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.