pycom04g12180

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000911
Physical Location & Seq
Reverse (-)
15165088 .. 15167724
2637 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g12180.6

Sequence Viewer

Length: 1407 bp
ATGGCTGCTTCAATCTCAGCAGCAACCATCATCGCCGTCAACAGAGCACCGTTGAACTTCAATGGCTCTGCTTTCTTAGGCAGTAACAGCTTGAAGAAGGTGAGCTCCAAGATCATCAGCCAGCCAAAGATACGTTCACCTGGGAATTTCATGGTTGCTGCAGAATACGATGAGTCGAAGCAGACCTCCAAAGACAGGTGGGGAGGGCTTGCCTTTGACGCTTCAGATGACCAGCAGGACATCACCAGAGGAAAGGGCATGGTGGACACGCTTTTCCAAGCTCCTATGGGCAGTGGGACCCACTACGCCGTCATGAGCTCCTACGATTACATCAGTACTGGTCTTCGCCAATTGGACAACATGATGGACGGCTTCTACATTGCTCCAGCTTTCATGGACAAACTTGTTGTTCACATTACTAAGAACTTTCTGAACCTGCCCAACATTAAGGTTCCTCTTATTTTGGGTGTCTGGGGAGGCAAAGGTCAAGGAAAGTCATTCCAATGTGAGCTTGTCTTTGCTAAGATGGGAATCAACCCTATTATGATGAGTGCTGGAGAATTGGAAAGTGGAAATGCCGGGGAGCCAGCAAAACTGATCAGGCAAAGGTACCGCGAGGCAGCAGACATCATCAGCAAGGGCAAAATGTGCTGCCTCTTCATCAATGATTTAGATGCAGGAGCTGGAAGACTGGGTGGAACTACTCAATACACTGTCAACAATCAAATGGTGAATGCTACCCTTATGAACATTGCTGACAACCCAACCAATGTCCAACTCCCTGGTATGTATAACAAGCAGGAGAATCCTCGCGTTCCCATCATAGTCACCGGTAATGACTTCTCCACATTGTACGCACCTCTCATCCGCGATGGGCGTATGGAGAAGTTCTACTGGGCCCCTACTCGTGAAGACTGCATTGGTGTGTGCATGGGAATTTTTCGCGCTGACAATGTTCCTAGAGGTGACATTGTCAAGCTCGTTGACACCTTCCCTGGTCAGTCCATTGATTTCTTTGGTGCCATCAGGGCCAGAGTTTATGATGATGAAGTGAGGAAATGGATTGCAAGTGTTGGGGTAGAAGGCATCGGGAAGAGGCTAGTGAACTCGAAAGAGGCTCCCCCGACTTTTGATCAACCGAAGATGACTCTTGAGAAGCTGCTTGAGTATGGAAACCTGCTGGTGCAAGAGCAGGACAATGTGAAGAGAGTCCAATTGTCCGACAAGTACTTGAAAGAGGCAGCTCTTGGGGATGCCAATGACGATGCCATAAAGAGTGGAAGTTTCTACGGCAAAGCAGCACAACAGGTTAACCTGCCTGTCCCTGAAGGCTGCACTGATCCATCTGCTGCGAACTACGATCCCACGGCGAGGAGCGACGACGGAAGTTGCCTGTACCAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

469

Amino Acids

51.19

Weight (kDa)

5.93

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 444, 1185, 1323
Acc65I GGTACC 1 cut(s) 609
AccB1I GGYRCC 2 cut(s) 609, 1019
AccII CGCG 4 cut(s) 615, 813, 870, 945
AciI CCGC 2 cut(s) 613, 868
AclWI GGATC 2 cut(s) 1334, 1355
AcsI RAATTY 2 cut(s) 145, 936
AcuI CTGAAG 2 cut(s) 207, 1347
AfaI GTAC 5 cut(s) 337, 611, 854, 1229, 1397
AfiI CCNNNNNNNGG 2 cut(s) 195, 1371
AgeI ACCGGT 1 cut(s) 830
AgsI TTSAA 5 cut(s) 12, 55, 61, 94, 1234
AjnI CCWGG 3 cut(s) 139, 781, 994
AjuI GAANNNNNNNTTGG 4 cut(s) 118, 150, 903, 935
Alw21I GWGCWC 3 cut(s) 49, 107, 320
AlwI GGATC 2 cut(s) 1334, 1355
AlwNI CAGNNNCTG 1 cut(s) 683
AoxI GGCC 2 cut(s) 897, 1029
ApaI GGGCCC 1 cut(s) 901
ApoI RAATTY 2 cut(s) 145, 936
AsiGI ACCGGT 1 cut(s) 830
Asp718I GGTACC 1 cut(s) 609
AspLEI GCGC 1 cut(s) 947
AspS9I GGNCC 4 cut(s) 297, 897, 898, 1029
AsuC2I CCSGG 1 cut(s) 580
AsuHPI GGTGA 6 cut(s) 112, 129, 235, 742, 820, 977
AvaII GGWCC 1 cut(s) 297
BaeGI GKGCMC 1 cut(s) 901
BanI GGYRCC 2 cut(s) 609, 1019
BanII GRGCYC 3 cut(s) 107, 320, 901
BauI CACGAG 1 cut(s) 906
BbsI GAAGAC 3 cut(s) 335, 694, 918
Bbv12I GWGCWC 3 cut(s) 49, 107, 320
BbvI GCAGC 9 cut(s) 32, 145, 632, 638, 1146, 1253, 1310, 1319, 1336
BccI CCATC 7 cut(s) 35, 358, 520, 827, 866, 1031, 1351
BceAI ACGGC 5 cut(s) 20, 293, 385, 1306, 1383
BciT130I CCWGG 3 cut(s) 141, 783, 996
BclI TGATCA 2 cut(s) 597, 1132
BcnI CCSGG 1 cut(s) 580
BfaI CTAG 2 cut(s) 960, 1100
BfmI CTRYAG 1 cut(s) 159
BfuAI ACCTGC 3 cut(s) 444, 1185, 1323
BglI GCCNNNNNGGC 1 cut(s) 1028
BmcAI AGTACT 2 cut(s) 337, 1229
Bme1390I CCNGG 4 cut(s) 141, 580, 783, 996
Bme18I GGWCC 1 cut(s) 297
BmgT120I GGNCC 4 cut(s) 297, 897, 898, 1029
BmiI GGNNCC 9 cut(s) 298, 299, 453, 585, 611, 899, 900, 1021, 1119
BmrFI CCNGG 4 cut(s) 141, 580, 783, 996
BmrI ACTGGG 2 cut(s) 701, 904
BmsI GCATC 4 cut(s) 664, 1095, 1243, 1255
BmuI ACTGGG 2 cut(s) 701, 904
BpiI GAAGAC 3 cut(s) 335, 694, 918
BpmI CTGGAG 2 cut(s) 369, 576
BpuEI CTTGAG 2 cut(s) 1172, 1184
BpuMI CCSGG 1 cut(s) 580
BsaBI GATNNNNATC 1 cut(s) 530
BsaJI CCNNGG 5 cut(s) 140, 579, 781, 994, 1365
BsaWI WCCGGW 1 cut(s) 830
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 1371
Bse118I RCCGGY 1 cut(s) 830
Bse1I ACTGG 3 cut(s) 343, 696, 899
Bse3DI GCAATG 2 cut(s) 378, 750
Bse8I GATNNNNATC 1 cut(s) 530
BseBI CCWGG 3 cut(s) 141, 783, 996
BseDI CCNNGG 5 cut(s) 140, 579, 781, 994, 1365
BseGI GGATG 2 cut(s) 864, 1258
BseJI GATNNNNATC 1 cut(s) 530
BseLI CCNNNNNNNGG 2 cut(s) 195, 1371
BseMI GCAATG 2 cut(s) 378, 750
BseMII CTCAG 1 cut(s) 30
BseNI ACTGG 3 cut(s) 343, 696, 899
BseRI GAGGAG 1 cut(s) 1387
BseSI GKGCMC 1 cut(s) 901
BseXI GCAGC 9 cut(s) 32, 145, 632, 638, 1146, 1253, 1310, 1319, 1336
BsgI GTGCAG 1 cut(s) 1318
Bsh1236I CGCG 4 cut(s) 615, 813, 870, 945
BshFI GGCC 2 cut(s) 899, 1031
BshNI GGYRCC 2 cut(s) 609, 1019
BshTI ACCGGT 1 cut(s) 830
BsiHKAI GWGCWC 3 cut(s) 49, 107, 320
BsiSI CCGG 2 cut(s) 579, 831
BslFI GGGAC 2 cut(s) 310, 1307
BslI CCNNNNNNNGG 2 cut(s) 195, 1371
BsmFI GGGAC 2 cut(s) 310, 1307
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 2 cut(s) 899, 1031
Bsp120I GGGCCC 1 cut(s) 897
Bsp1286I GDGCHC 4 cut(s) 49, 107, 320, 901
Bsp143I GATC 5 cut(s) 111, 597, 1132, 1339, 1360
BspACI CCGC 2 cut(s) 613, 868
BspANI GGCC 2 cut(s) 899, 1031
BspCNI CTCAG 1 cut(s) 29
BspFNI CGCG 4 cut(s) 615, 813, 870, 945
BspHI TCATGA 1 cut(s) 312
BspLI GGNNCC 9 cut(s) 298, 299, 453, 585, 611, 899, 900, 1021, 1119
BspMAI CTGCAG 1 cut(s) 163
BspMI ACCTGC 3 cut(s) 444, 1185, 1323
BspPI GGATC 2 cut(s) 1334, 1355
BspT107I GGYRCC 2 cut(s) 609, 1019
BsrDI GCAATG 2 cut(s) 378, 750
BsrFI RCCGGY 1 cut(s) 830
BsrI ACTGG 3 cut(s) 343, 696, 899
BssAI RCCGGY 1 cut(s) 830
BssECI CCNNGG 5 cut(s) 140, 579, 781, 994, 1365
BssMI GATC 5 cut(s) 111, 597, 1132, 1339, 1360
BssSI CACGAG 1 cut(s) 906
Bst2BI CACGAG 1 cut(s) 906
Bst2UI CCWGG 3 cut(s) 141, 783, 996
Bst4CI ACNGT 2 cut(s) 51, 715
Bst6I CTCTTC 3 cut(s) 662, 1088, 1199
BstAPI GCANNNNNTGC 1 cut(s) 648
BstC8I GCNNGC 3 cut(s) 122, 210, 588
BstDEI CTNAG 4 cut(s) 16, 76, 420, 522
BstDSI CCRYGG 1 cut(s) 1365
BstF5I GGATG 2 cut(s) 864, 1258
BstFNI CGCG 4 cut(s) 615, 813, 870, 945
BstHHI GCGC 1 cut(s) 947
BstKTI GATC 5 cut(s) 114, 600, 1135, 1342, 1363
BstMBI GATC 5 cut(s) 111, 597, 1132, 1339, 1360
BstMWI GCNNNNNNNGC 4 cut(s) 87, 218, 648, 1028
BstNI CCWGG 3 cut(s) 141, 783, 996
BstSCI CCNGG 4 cut(s) 139, 578, 781, 994
BstSFI CTRYAG 1 cut(s) 159
BstSLI GKGCMC 1 cut(s) 901
BstUI CGCG 4 cut(s) 615, 813, 870, 945
BstV1I GCAGC 9 cut(s) 32, 145, 632, 638, 1146, 1253, 1310, 1319, 1336
BstV2I GAAGAC 3 cut(s) 335, 694, 918
BstXI CCANNNNNNTGG 1 cut(s) 782
BsuRI GGCC 2 cut(s) 899, 1031
BtgI CCRYGG 1 cut(s) 1365
BtgZI GCGATG 2 cut(s) 16, 885
BtsCI GGATG 2 cut(s) 864, 1258
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 3 cut(s) 298, 711, 1335
BveI ACCTGC 3 cut(s) 444, 1185, 1323
Cac8I GCNNGC 3 cut(s) 122, 210, 588
CaiI CAGNNNCTG 1 cut(s) 683
CciI TCATGA 1 cut(s) 312
CfoI GCGC 1 cut(s) 947
Cfr10I RCCGGY 1 cut(s) 830
Cfr13I GGNCC 4 cut(s) 297, 897, 898, 1029
CseI GACGC 1 cut(s) 227
Csp6I GTAC 5 cut(s) 336, 610, 853, 1228, 1396
CspAI ACCGGT 1 cut(s) 830
CviAII CATG 6 cut(s) 151, 259, 313, 361, 394, 931
CviQI GTAC 5 cut(s) 336, 610, 853, 1228, 1396
DdeI CTNAG 4 cut(s) 16, 76, 420, 522
DpnI GATC 5 cut(s) 113, 599, 1134, 1341, 1362
DpnII GATC 5 cut(s) 111, 597, 1132, 1339, 1360
Eam1104I CTCTTC 3 cut(s) 662, 1088, 1199
EarI CTCTTC 3 cut(s) 662, 1088, 1199
Ecl136II GAGCTC 2 cut(s) 105, 318
Eco24I GRGCYC 3 cut(s) 107, 320, 901
Eco47I GGWCC 1 cut(s) 297
Eco53kI GAGCTC 2 cut(s) 105, 318
Eco57I CTGAAG 2 cut(s) 207, 1347
EcoICRI GAGCTC 2 cut(s) 105, 318
EcoO109I RGGNCCY 2 cut(s) 297, 898
EcoRII CCWGG 3 cut(s) 139, 781, 994
EcoT38I GRGCYC 3 cut(s) 107, 320, 901
FaeI CATG 6 cut(s) 154, 262, 316, 364, 397, 934
FaqI GGGAC 2 cut(s) 310, 1307
FatI CATG 6 cut(s) 150, 258, 312, 360, 393, 930
FbaI TGATCA 2 cut(s) 597, 1132
FokI GGATG 2 cut(s) 851, 1265
FriOI GRGCYC 3 cut(s) 107, 320, 901
FspBI CTAG 2 cut(s) 960, 1100
GlaI GCGC 1 cut(s) 946
GsuI CTGGAG 2 cut(s) 369, 576
HaeIII GGCC 2 cut(s) 899, 1031
HapII CCGG 2 cut(s) 579, 831
HgaI GACGC 1 cut(s) 227
HhaI GCGC 1 cut(s) 947
Hin1II CATG 6 cut(s) 154, 262, 316, 364, 397, 934
Hin6I GCGC 1 cut(s) 945
HinP1I GCGC 1 cut(s) 945
HincII GTYRAC 4 cut(s) 40, 718, 985, 1312
HindII GTYRAC 4 cut(s) 40, 718, 985, 1312
HinfI GANTC 5 cut(s) 173, 531, 805, 1147, 1209
HpaI GTTAAC 1 cut(s) 1312
HpaII CCGG 2 cut(s) 579, 831
HphI GGTGA 6 cut(s) 112, 129, 235, 742, 820, 977
Hpy166II GTNNAC 8 cut(s) 40, 137, 265, 412, 718, 985, 1105, 1312
Hpy188I TCNGA 3 cut(s) 226, 432, 1222
Hpy188III TCNNGA 4 cut(s) 313, 908, 1090, 1151
Hpy8I GTNNAC 8 cut(s) 40, 137, 265, 412, 718, 985, 1105, 1312
Hpy99I CGWCG 2 cut(s) 1382, 1385
HpyAV CCTTC 4 cut(s) 91, 1000, 1076, 1322
HpyCH4III ACNGT 2 cut(s) 51, 715
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 7 cut(s) 161, 677, 918, 930, 1067, 1186, 1335
HpyF10VI GCNNNNNNNGC 4 cut(s) 87, 218, 648, 1028
HpyF3I CTNAG 4 cut(s) 16, 76, 420, 522
HpySE526I ACGT 1 cut(s) 133
Hsp92II CATG 6 cut(s) 154, 262, 316, 364, 397, 934
HspAI GCGC 1 cut(s) 945
KflI GGGWCCC 1 cut(s) 297
KpnI GGTACC 1 cut(s) 613
Ksp22I TGATCA 2 cut(s) 597, 1132
KspAI GTTAAC 1 cut(s) 1312
Kzo9I GATC 5 cut(s) 111, 597, 1132, 1339, 1360
LmnI GCTCC 8 cut(s) 110, 286, 323, 388, 583, 680, 1123, 1374
Lsp1109I GCAGC 9 cut(s) 32, 145, 632, 638, 1146, 1253, 1310, 1319, 1336
LweI GCATC 4 cut(s) 664, 1095, 1243, 1255
MaeI CTAG 2 cut(s) 960, 1100
MaeII ACGT 1 cut(s) 133
MaeIII GTNAC 3 cut(s) 83, 826, 965
MalI GATC 5 cut(s) 113, 599, 1134, 1341, 1362
MboI GATC 5 cut(s) 111, 597, 1132, 1339, 1360
MboII GAAGA 8 cut(s) 106, 335, 649, 699, 923, 1105, 1153, 1216
MfeI CAATTG 2 cut(s) 350, 1214
MhlI GDGCHC 4 cut(s) 49, 107, 320, 901
MluCI AATT 6 cut(s) 145, 350, 560, 936, 1214, 1400
MlyI GAGTC 3 cut(s) 182, 1141, 1218
MmeI TCCRAC 2 cut(s) 799, 1245
MseI TTAA 2 cut(s) 447, 1311
MslI CAYNNNNRTG 2 cut(s) 502, 923
MspI CCGG 2 cut(s) 579, 831
MspR9I CCNGG 4 cut(s) 141, 580, 783, 996
MunI CAATTG 2 cut(s) 350, 1214
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 3 cut(s) 141, 783, 996
MvnI CGCG 4 cut(s) 615, 813, 870, 945
MwoI GCNNNNNNNGC 4 cut(s) 87, 218, 648, 1028
NciI CCSGG 1 cut(s) 580
NdeII GATC 5 cut(s) 111, 597, 1132, 1339, 1360
NlaIII CATG 6 cut(s) 154, 262, 316, 364, 397, 934
NlaIV GGNNCC 9 cut(s) 298, 299, 453, 585, 611, 899, 900, 1021, 1119
NmuCI GTSAC 2 cut(s) 826, 965
PagI TCATGA 1 cut(s) 312
PctI GAATGC 1 cut(s) 739
PfeI GAWTC 2 cut(s) 531, 805
PflFI GACNNNGTC 1 cut(s) 971
PinAI ACCGGT 1 cut(s) 830
PleI GAGTC 3 cut(s) 181, 1141, 1217
PpsI GAGTC 3 cut(s) 181, 1141, 1217
PpuMI RGGWCCY 1 cut(s) 297
Psp124BI GAGCTC 2 cut(s) 107, 320
Psp5II RGGWCCY 1 cut(s) 297
Psp6I CCWGG 3 cut(s) 139, 781, 994
PspGI CCWGG 3 cut(s) 139, 781, 994
PspN4I GGNNCC 9 cut(s) 298, 299, 453, 585, 611, 899, 900, 1021, 1119
PspOMI GGGCCC 1 cut(s) 897
PspPI GGNCC 4 cut(s) 297, 897, 898, 1029
PspPPI RGGWCCY 1 cut(s) 297
PstI CTGCAG 1 cut(s) 163
PstNI CAGNNNCTG 1 cut(s) 683
PsyI GACNNNGTC 1 cut(s) 971
RsaI GTAC 5 cut(s) 337, 611, 854, 1229, 1397
RsaNI GTAC 5 cut(s) 336, 610, 853, 1228, 1396
RseI CAYNNNNRTG 2 cut(s) 502, 923
SacI GAGCTC 2 cut(s) 107, 320
SaqAI TTAA 2 cut(s) 447, 1311
Sau3AI GATC 5 cut(s) 111, 597, 1132, 1339, 1360
Sau96I GGNCC 4 cut(s) 297, 897, 898, 1029
ScaI AGTACT 2 cut(s) 337, 1229
SchI GAGTC 3 cut(s) 182, 1141, 1218
ScrFI CCNGG 4 cut(s) 141, 580, 783, 996
SduI GDGCHC 4 cut(s) 49, 107, 320, 901
SfaNI GCATC 4 cut(s) 664, 1095, 1243, 1255
SfcI CTRYAG 1 cut(s) 159
SinI GGWCC 1 cut(s) 297
SmiMI CAYNNNNRTG 2 cut(s) 502, 923
SmlI CTYRAG 2 cut(s) 1151, 1163
SmoI CTYRAG 2 cut(s) 1151, 1163
Sse9I AATT 6 cut(s) 145, 350, 560, 936, 1214, 1400
SsiI CCGC 2 cut(s) 613, 868
SspMI CTAG 2 cut(s) 960, 1100
SstI GAGCTC 2 cut(s) 107, 320
StyD4I CCNGG 4 cut(s) 139, 578, 781, 994
TaaI ACNGT 2 cut(s) 51, 715
TaiI ACGT 1 cut(s) 136
TaqI TCGA 2 cut(s) 176, 1109
TasI AATT 6 cut(s) 145, 350, 560, 936, 1214, 1400
TatI WGTACW 2 cut(s) 335, 1227
TfiI GAWTC 2 cut(s) 531, 805
Tru1I TTAA 2 cut(s) 447, 1311
Tru9I TTAA 2 cut(s) 447, 1311
TscAI CASTG 3 cut(s) 298, 718, 1342
TseFI GTSAC 2 cut(s) 826, 965
Tsp45I GTSAC 2 cut(s) 826, 965
TspDTI ATGAA 5 cut(s) 139, 382, 649, 761, 1062
TspGWI ACGGA 1 cut(s) 1398
TspRI CASTG 3 cut(s) 298, 718, 1342
Tth111I GACNNNGTC 1 cut(s) 971
VpaK11BI GGWCC 1 cut(s) 297
XapI RAATTY 2 cut(s) 145, 936
XspI CTAG 2 cut(s) 960, 1100
ZrmI AGTACT 2 cut(s) 337, 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.