pycom04g17700

ATP-dependent Clp protease proteolytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0010248
Physical Location & Seq
Reverse (-)
19988969 .. 19991735
2767 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 984 bp
ATGGCGAGCAGTTTGCAGCTGCCAATCTCCATATCGTCAGCTCCTGCGTCGGCGTTTAGACCCAGAAATGCTCGGAAGCTTACAGCATTTTGCTCCGTTAACGCCAAAGCAACGGCCAAAATCCCAACTCCTCCAATAAACCCCAAGGACCCCTTTCTTTCGAGGCTCGCTGCGGTGGCTGCCAAGTCCCCGGAAACCTTGCTGAACCGGCCGCCTCAAAATTCCGACTCTCTCCCGTACTTGGATGTGTTTGATGAACCTATTCTCATGGCCACTCCTGCCCAAGTGGAAAGATCGGTTTCTTACAACGAGCATAGGCCCAAGAAGCCACCTCCAGACCTGCCCTCGTTGTTACTTCACGGAAGAATAGTTTATATTGGCATGCCTTTAGTGCCAGCAGTCACAGAGCTTATTGTTGCGGAATTGATGTATCTGCAATGGATCGATCCTAAACAACCAATATATATTTATATTAATTCGACGGGAACCACCCGTGATGATGGTGAAACCGTTGGCATGGAGTCAGAGGGATTTGCTATCTATGATGCTATGATGCAGTTGAAAAATGAGATACATACGGTTGCTGTTGGGGCCGCTATAGGTCAAGCATGTCTTTTGCTTTCAGCAGGAACTAAGGGCAAACGATTTATGATGCCACATGCCAAAGCAATGATCCAGCAACCTCGTGTCCCATCATCTGGACTGATGCCTGCTAGTGATGTTCTTATTCGTGCAAAAGAGGTAATCACAAATAGGGACATTCTCGTAAAGCTTCTGGCCAAACACACTGGAAATTCAGAGGAGGCTGTTGCTAAAGTGATGAGAAGACCATTTTATATGGATTCTACAACTGCTAAAGAATTCGGGGTGATTGACAAGATCCTTTGGCGTGGTCAAGAAAAGATAATGGCAGATGTAGCCTCCCCTGAGGAATGGGATAATAAAGCTGGCGTCAAAGTTGTAGATGAACTCGGTGGTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLP_protease

Protein Analysis

328

Amino Acids

35.74

Weight (kDa)

8.69

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 116 - 295 6.5e-43 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 348
AccB7I CCANNNNNTGG 1 cut(s) 698
AciI CCGC 4 cut(s) 173, 212, 419, 594
AclWI GGATC 4 cut(s) 440, 449, 667, 874
AcoI YGGCCR 4 cut(s) 114, 209, 270, 777
AcsI RAATTY 3 cut(s) 220, 793, 860
AcyI GRCGYC 1 cut(s) 951
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 2 cut(s) 241, 698
AgsI TTSAA 1 cut(s) 562
AluBI AGCT 6 cut(s) 19, 41, 79, 409, 772, 947
AluI AGCT 6 cut(s) 19, 41, 79, 409, 772, 947
AlwI GGATC 4 cut(s) 440, 449, 667, 874
AlwNI CAGNNNCTG 1 cut(s) 44
AoxI GGCC 6 cut(s) 114, 209, 270, 317, 591, 777
ApeKI GCWGC 4 cut(s) 16, 19, 170, 179
ApoI RAATTY 3 cut(s) 220, 793, 860
AseI ATTAAT 1 cut(s) 474
Asp700I GAANNNNTTC 1 cut(s) 261
AspS9I GGNCC 3 cut(s) 148, 318, 591
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 2 cut(s) 515, 880
AvaII GGWCC 1 cut(s) 148
AxyI CCTNAGG 1 cut(s) 927
BalI TGGCCA 2 cut(s) 272, 779
BauI CACGAG 1 cut(s) 684
BbsI GAAGAC 1 cut(s) 832
BbvI GCAGC 4 cut(s) 6, 28, 157, 166
BccI CCATC 2 cut(s) 494, 700
BceAI ACGGC 1 cut(s) 129
BcgI CGANNNNNNTGC 1 cut(s) 29
BcnI CCSGG 1 cut(s) 191
BfaI CTAG 1 cut(s) 714
BfmI CTRYAG 1 cut(s) 597
BfuAI ACCTGC 1 cut(s) 348
BisI GCNGC 6 cut(s) 17, 20, 171, 180, 212, 594
BlsI GCNGC 6 cut(s) 18, 21, 172, 181, 213, 595
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 3 cut(s) 148, 318, 591
BmiI GGNNCC 3 cut(s) 150, 487, 592
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 4 cut(s) 535, 543, 642, 696
BpiI GAAGAC 1 cut(s) 832
BpmI CTGGAG 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 191
Bsa29I ATCGAT 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 951
BsaJI CCNNGG 2 cut(s) 144, 189
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 698
Bse118I RCCGGY 1 cut(s) 207
Bse1I ACTGG 1 cut(s) 793
Bse21I CCTNAGG 1 cut(s) 927
Bse3DI GCAATG 2 cut(s) 443, 675
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 2 cut(s) 144, 189
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 2 cut(s) 241, 698
BseMI GCAATG 2 cut(s) 443, 675
BseMII CTCAG 1 cut(s) 918
BseNI ACTGG 1 cut(s) 793
BseRI GAGGAG 2 cut(s) 120, 815
BseX3I CGGCCG 1 cut(s) 209
BseXI GCAGC 4 cut(s) 6, 28, 157, 166
Bsh1285I CGRYCG 1 cut(s) 212
BshFI GGCC 6 cut(s) 116, 211, 272, 319, 593, 779
BshVI ATCGAT 1 cut(s) 444
BsiEI CGRYCG 1 cut(s) 212
BsiSI CCGG 2 cut(s) 191, 208
BslFI GGGAC 3 cut(s) 172, 674, 770
BslI CCNNNNNNNGG 2 cut(s) 241, 698
BsmFI GGGAC 3 cut(s) 172, 674, 770
BsnI GGCC 6 cut(s) 116, 211, 272, 319, 593, 779
Bsp143I GATC 5 cut(s) 293, 441, 445, 672, 879
BspACI CCGC 4 cut(s) 173, 212, 419, 594
BspANI GGCC 6 cut(s) 116, 211, 272, 319, 593, 779
BspCNI CTCAG 1 cut(s) 919
BspDI ATCGAT 1 cut(s) 444
BspLI GGNNCC 3 cut(s) 150, 487, 592
BspMI ACCTGC 1 cut(s) 348
BspPI GGATC 4 cut(s) 440, 449, 667, 874
BsrDI GCAATG 2 cut(s) 443, 675
BsrFI RCCGGY 1 cut(s) 207
BsrI ACTGG 1 cut(s) 793
BssAI RCCGGY 1 cut(s) 207
BssECI CCNNGG 2 cut(s) 144, 189
BssMI GATC 5 cut(s) 293, 441, 445, 672, 879
BssNI GRCGYC 1 cut(s) 951
BssSI CACGAG 1 cut(s) 684
BssT1I CCWWGG 1 cut(s) 144
Bst2BI CACGAG 1 cut(s) 684
Bst4CI ACNGT 2 cut(s) 511, 580
BstACI GRCGYC 1 cut(s) 951
BstC8I GCNNGC 6 cut(s) 7, 168, 383, 396, 711, 949
BstDEI CTNAG 2 cut(s) 633, 927
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 5 cut(s) 296, 444, 448, 675, 882
BstMBI GATC 5 cut(s) 293, 441, 445, 672, 879
BstMCI CGRYCG 1 cut(s) 212
BstMWI GCNNNNNNNGC 8 cut(s) 176, 179, 208, 278, 325, 391, 590, 917
BstNSI RCATGY 3 cut(s) 385, 612, 662
BstSCI CCNGG 1 cut(s) 189
BstSFI CTRYAG 1 cut(s) 597
BstV1I GCAGC 4 cut(s) 6, 28, 157, 166
BstV2I GAAGAC 1 cut(s) 832
BstX2I RGATCY 1 cut(s) 879
BstYI RGATCY 1 cut(s) 879
BstZI CGGCCG 1 cut(s) 209
Bsu15I ATCGAT 1 cut(s) 444
Bsu36I CCTNAGG 1 cut(s) 927
BsuRI GGCC 6 cut(s) 116, 211, 272, 319, 593, 779
BsuTUI ATCGAT 1 cut(s) 444
BtsCI GGATG 1 cut(s) 250
BtsIMutI CAGTG 1 cut(s) 786
BveI ACCTGC 1 cut(s) 348
Cac8I GCNNGC 6 cut(s) 7, 168, 383, 396, 711, 949
CaiI CAGNNNCTG 1 cut(s) 44
Cfr10I RCCGGY 1 cut(s) 207
Cfr13I GGNCC 3 cut(s) 148, 318, 591
ClaI ATCGAT 1 cut(s) 444
CseI GACGC 2 cut(s) 36, 940
Csp6I GTAC 1 cut(s) 238
CviAII CATG 5 cut(s) 268, 382, 517, 609, 659
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 2 cut(s) 633, 927
DpnI GATC 5 cut(s) 295, 443, 447, 674, 881
DpnII GATC 5 cut(s) 293, 441, 445, 672, 879
EaeI YGGCCR 4 cut(s) 114, 209, 270, 777
EagI CGGCCG 1 cut(s) 209
EclXI CGGCCG 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 148
Eco52I CGGCCG 1 cut(s) 209
Eco81I CCTNAGG 1 cut(s) 927
EcoO109I RGGNCCY 1 cut(s) 148
EcoRI GAATTC 1 cut(s) 860
EcoT14I CCWWGG 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 5 cut(s) 271, 385, 520, 612, 662
FalI AAGNNNNNCTT 4 cut(s) 137, 169, 597, 629
FaqI GGGAC 3 cut(s) 172, 674, 770
FatI CATG 5 cut(s) 267, 381, 516, 608, 658
Fnu4HI GCNGC 6 cut(s) 17, 20, 171, 180, 212, 594
FokI GGATG 1 cut(s) 257
Fsp4HI GCNGC 6 cut(s) 17, 20, 171, 180, 212, 594
FspBI CTAG 1 cut(s) 714
GluI GCNGC 6 cut(s) 17, 20, 171, 180, 212, 594
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 6 cut(s) 116, 211, 272, 319, 593, 779
HapII CCGG 2 cut(s) 191, 208
HgaI GACGC 2 cut(s) 36, 940
Hin1I GRCGYC 1 cut(s) 951
Hin1II CATG 5 cut(s) 271, 385, 520, 612, 662
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 2 cut(s) 77, 770
HinfI GANTC 3 cut(s) 227, 521, 842
HpaI GTTAAC 1 cut(s) 100
HpaII CCGG 2 cut(s) 191, 208
HphI GGTGA 2 cut(s) 515, 880
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 4 cut(s) 75, 226, 526, 799
Hpy188III TCNNGA 3 cut(s) 335, 699, 896
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 2 cut(s) 52, 484
HpyCH4III ACNGT 2 cut(s) 511, 580
HpyCH4V TGCA 4 cut(s) 16, 436, 556, 734
HpyF10VI GCNNNNNNNGC 8 cut(s) 176, 179, 208, 278, 325, 391, 590, 917
HpyF3I CTNAG 2 cut(s) 633, 927
Hsp92I GRCGYC 1 cut(s) 951
Hsp92II CATG 5 cut(s) 271, 385, 520, 612, 662
KspAI GTTAAC 1 cut(s) 100
Kzo9I GATC 5 cut(s) 293, 441, 445, 672, 879
LmnI GCTCC 2 cut(s) 46, 98
Lsp1109I GCAGC 4 cut(s) 6, 28, 157, 166
LweI GCATC 4 cut(s) 535, 543, 642, 696
MaeI CTAG 1 cut(s) 714
MaeIII GTNAC 2 cut(s) 351, 400
MalI GATC 5 cut(s) 295, 443, 447, 674, 881
MboI GATC 5 cut(s) 293, 441, 445, 672, 879
MboII GAAGA 2 cut(s) 375, 837
MflI RGATCY 1 cut(s) 879
MlsI TGGCCA 2 cut(s) 272, 779
MluCI AATT 5 cut(s) 220, 422, 475, 793, 860
MluNI TGGCCA 2 cut(s) 272, 779
MlyI GAGTC 2 cut(s) 221, 530
MmeI TCCRAC 1 cut(s) 249
Mox20I TGGCCA 2 cut(s) 272, 779
MroXI GAANNNNTTC 1 cut(s) 261
MscI TGGCCA 2 cut(s) 272, 779
MseI TTAA 2 cut(s) 99, 474
Msp20I TGGCCA 2 cut(s) 272, 779
MspA1I CMGCKG 1 cut(s) 19
MspI CCGG 2 cut(s) 191, 208
MspR9I CCNGG 1 cut(s) 191
MwoI GCNNNNNNNGC 8 cut(s) 176, 179, 208, 278, 325, 391, 590, 917
NciI CCSGG 1 cut(s) 191
NdeII GATC 5 cut(s) 293, 441, 445, 672, 879
NlaIII CATG 5 cut(s) 271, 385, 520, 612, 662
NlaIV GGNNCC 3 cut(s) 150, 487, 592
NmuCI GTSAC 1 cut(s) 400
NspI RCATGY 3 cut(s) 385, 612, 662
PaeI GCATGC 1 cut(s) 385
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 1 cut(s) 842
PflMI CCANNNNNTGG 1 cut(s) 698
PkrI GCNGC 6 cut(s) 18, 21, 172, 181, 213, 595
PleI GAGTC 2 cut(s) 221, 529
PpsI GAGTC 2 cut(s) 221, 529
PpuMI RGGWCCY 1 cut(s) 148
PshBI ATTAAT 1 cut(s) 474
Psp5II RGGWCCY 1 cut(s) 148
PspN4I GGNNCC 3 cut(s) 150, 487, 592
PspPI GGNCC 3 cut(s) 148, 318, 591
PspPPI RGGWCCY 1 cut(s) 148
PstNI CAGNNNCTG 1 cut(s) 44
PsuI RGATCY 1 cut(s) 879
PvuII CAGCTG 1 cut(s) 19
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SaqAI TTAA 2 cut(s) 99, 474
SatI GCNGC 6 cut(s) 17, 20, 171, 180, 212, 594
Sau3AI GATC 5 cut(s) 293, 441, 445, 672, 879
Sau96I GGNCC 3 cut(s) 148, 318, 591
SchI GAGTC 2 cut(s) 221, 530
ScrFI CCNGG 1 cut(s) 191
SfaNI GCATC 4 cut(s) 535, 543, 642, 696
SfcI CTRYAG 1 cut(s) 597
SinI GGWCC 1 cut(s) 148
SphI GCATGC 1 cut(s) 385
Sse9I AATT 5 cut(s) 220, 422, 475, 793, 860
SsiI CCGC 4 cut(s) 173, 212, 419, 594
SspMI CTAG 1 cut(s) 714
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 511, 580
TaqI TCGA 3 cut(s) 161, 444, 479
TasI AATT 5 cut(s) 220, 422, 475, 793, 860
TauI GCSGC 2 cut(s) 214, 596
TfiI GAWTC 1 cut(s) 842
Tru1I TTAA 2 cut(s) 99, 474
Tru9I TTAA 2 cut(s) 99, 474
TscAI CASTG 1 cut(s) 793
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 4 cut(s) 16, 19, 170, 179
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 2 cut(s) 270, 981
TspGWI ACGGA 2 cut(s) 85, 375
TspRI CASTG 1 cut(s) 793
Van91I CCANNNNNTGG 1 cut(s) 698
VpaK11BI GGWCC 1 cut(s) 148
VspI ATTAAT 1 cut(s) 474
XapI RAATTY 3 cut(s) 220, 793, 860
XceI RCATGY 3 cut(s) 385, 612, 662
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.