pycom04g17720

Cell cycle checkpoint control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0003050
Physical Location & Seq
Reverse (-)
20004063 .. 20007380
3318 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1329 bp
ATGGAGTTCAGTGTGAGCGGCAACGCTCTGAAGACCTTCGCCCGCTCCATCACGTGCCTCGCACGCATCGGCAACGAGCTCGCCGTCCAAGCTTCTCCTTCTCAGCTTGCTTTGCACTCTCTCAATACATCACGTTCTGCCTATCAATCGAGCACATTTAAGCCTGATTTCTTTGACGTCTATACTGTAACTGGTAATCAGGCTGTTTGCTCTGTGCTTAGGACACCTTTAGCAAGTATAGATAATTTGAGCGTGAAATTGCCTGATCCCGATGCATCAAAACTGCAATGGACTTTACAGTGCTTCAGTGGTATGAAAAAAACCTATTGGATTAGTTGCAATGTTGAACCCGATATACAACATTTGTCTCTTGATAGGAGAAAATTCCCAAGCAGCTTAGTTGTGAGACCCCGAGATCTGAACAGATTGCTTGCTAATTTTCAGTCATCGCTTCAGGAGATTACAGTCATAGCAACAGAACCCACTTCCTTGCCTTCTGATGCAGCAAGTGAAATTGGAGGAAAAGCTGTTGAACTTAGAAGTTATATTGACCCAACCAAAGACAGTGATTCGTCACTGCATACTCAACTATGGATAGATCCTACTGAAGAGTTTGTGCAGTATACCCACACTGGAGACCCCATAGATGTGACATTCGGTGTGAAGGAGTTGAAGGCGTTCCTTTCTTTCTGCGAGGGCTGTGAAATTGACATCCACTTATATTTTGAGAAAGCTGGACAGCCTATTCTAATGGCACCAAAGTTTGGTCTAGATGATGGGTCCACTTCAAATTTTGATGCTACACTTGTACTTGCAACCATGCTAGTGTCACAGCTGCATGAAACCAATCCAGCAGAACCTCCACAAGCAGCTACCGAAATGCACATTCGGGATGAGCATGGAACAGGGTCAGAAGCACGACGAGAAAGGTGTAGGCCAAGTGGCTCTGAGCATCCATCTGACCACACCAGAATTTGGTCTGATCTCTCAGGAAGTGGTGCAAGAAGTGGCAATGGCGCTGAAGGAGGACAGTTTCAAGGGGAAAGAAATTTGAATGCTAATGGACAGAGAGAAGTCCAAAGGATGAGTAAACTGCATATTTCGAATCCTGCATCTGCTGGGGGAAATGAGCCTGTGGATCCCAATGTCTGCCACCCTGAGGAAAATGATCATATGGAAGAACCTAGAGATAGATCACAAATTAACAGCGGTGGGTTTTCACAACACCATCCTAGTAACTGGGTAGATGCAGATGAAGACGACAGTGATGAGGATGAAGAGAATGACCTTTGCATTCAGGCAACACCACCATACTATGAAGAACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000076 GO:0000077 GO:0000228 GO:0000278 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0008408 GO:0009314 GO:0009628 GO:0009966 GO:0009967 GO:0009987 GO:0010212 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016787 GO:0016788 GO:0017124 GO:0019899 GO:0019900 GO:0019901 GO:0019904 GO:0022402 GO:0023051 GO:0023056 GO:0030896 GO:0031570 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0042826 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044773 GO:0044774 GO:0045786 GO:0045930 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071479 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0104004 GO:1901360 GO:1902229 GO:1902231 GO:1902531 GO:1902533 GO:1903047 GO:2001020 GO:2001022 GO:2001233 GO:2001235 GO:2001242 GO:2001244
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
Rad9

Protein Analysis

443

Amino Acids

48.76

Weight (kDa)

4.78

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad9 PF04139 13 - 274 1.5e-69 Rad9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
AccB1I GGYRCC 1 cut(s) 754
AccB7I CCANNNNNTGG 2 cut(s) 764, 975
AccBSI CCGCTC 2 cut(s) 18, 45
AccI GTMKAC 1 cut(s) 623
AciI CCGC 3 cut(s) 18, 43, 1209
AclWI GGATC 4 cut(s) 260, 593, 1133, 1146
AcsI RAATTY 4 cut(s) 383, 790, 972, 1048
AcuI CTGAAG 5 cut(s) 50, 289, 437, 627, 1041
AcvI CACGTG 1 cut(s) 54
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 1 cut(s) 810
AfiI CCNNNNNNNGG 3 cut(s) 764, 975, 1159
AgsI TTSAA 6 cut(s) 347, 533, 673, 789, 1037, 1054
AluBI AGCT 8 cut(s) 79, 92, 106, 396, 527, 734, 835, 872
AluI AGCT 8 cut(s) 79, 92, 106, 396, 527, 734, 835, 872
Alw21I GWGCWC 2 cut(s) 81, 155
Alw26I GTCTC 3 cut(s) 372, 400, 630
AlwI GGATC 4 cut(s) 260, 593, 1133, 1146
Ama87I CYCGRG 1 cut(s) 411
AoxI GGCC 1 cut(s) 935
ApeKI GCWGC 4 cut(s) 393, 503, 835, 869
ApoI RAATTY 4 cut(s) 383, 790, 972, 1048
Asp700I GAANNNNTTC 2 cut(s) 35, 677
AspLEI GCGC 1 cut(s) 1019
AspS9I GGNCC 1 cut(s) 780
AsuII TTCGAA 1 cut(s) 1103
AvaI CYCGRG 1 cut(s) 411
AvaII GGWCC 1 cut(s) 780
AxyI CCTNAGG 1 cut(s) 1158
BamHI GGATCC 1 cut(s) 1138
BanI GGYRCC 1 cut(s) 754
BanII GRGCYC 1 cut(s) 81
BbrPI CACGTG 1 cut(s) 54
BbsI GAAGAC 2 cut(s) 38, 1263
Bbv12I GWGCWC 2 cut(s) 81, 155
BbvI GCAGC 4 cut(s) 405, 515, 822, 881
BccI CCATC 4 cut(s) 56, 770, 964, 1236
BceAI ACGGC 1 cut(s) 68
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BclI TGATCA 1 cut(s) 1168
BcoDI GTCTC 3 cut(s) 372, 400, 630
BfaI CTAG 4 cut(s) 770, 824, 1185, 1233
BfoI RGCGCY 1 cut(s) 1020
BglII AGATCT 1 cut(s) 415
BisI GCNGC 5 cut(s) 19, 394, 504, 836, 870
BlsI GCNGC 5 cut(s) 20, 395, 505, 837, 871
Bme18I GGWCC 1 cut(s) 780
BmeT110I CYCGRG 1 cut(s) 411
BmgT120I GGNCC 1 cut(s) 780
BmiI GGNNCC 3 cut(s) 756, 781, 1140
BmrI ACTGGG 1 cut(s) 1249
BmsI GCATC 8 cut(s) 75, 262, 284, 490, 787, 961, 1121, 1237
BmuI ACTGGG 1 cut(s) 1249
BpiI GAAGAC 2 cut(s) 38, 1263
BpmI CTGGAG 1 cut(s) 654
Bpu10I CCTNAGC 1 cut(s) 218
Bpu14I TTCGAA 1 cut(s) 1103
BsaAI YACGTR 1 cut(s) 54
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 2 cut(s) 400, 630
Bsc4I CCNNNNNNNGG 3 cut(s) 764, 975, 1159
Bse1I ACTGG 3 cut(s) 196, 637, 1244
Bse21I CCTNAGG 1 cut(s) 1158
Bse3DI GCAATG 3 cut(s) 293, 346, 1018
BseGI GGATG 6 cut(s) 711, 898, 952, 1089, 1228, 1279
BseLI CCNNNNNNNGG 3 cut(s) 764, 975, 1159
BseMI GCAATG 3 cut(s) 293, 346, 1018
BseMII CTCAG 4 cut(s) 116, 939, 1002, 1149
BseNI ACTGG 3 cut(s) 196, 637, 1244
BseXI GCAGC 4 cut(s) 405, 515, 822, 881
BseYI CCCAGC 1 cut(s) 1118
BsgI GTGCAG 1 cut(s) 638
BshFI GGCC 1 cut(s) 937
BshNI GGYRCC 1 cut(s) 754
BsiHKAI GWGCWC 2 cut(s) 81, 155
BsiHKCI CYCGRG 1 cut(s) 411
BslI CCNNNNNNNGG 3 cut(s) 764, 975, 1159
BsmAI GTCTC 3 cut(s) 372, 400, 630
BsmI GAATGC 2 cut(s) 1060, 1293
BsnI GGCC 1 cut(s) 937
Bso31I GGTCTC 2 cut(s) 400, 630
BsoBI CYCGRG 1 cut(s) 411
Bsp119I TTCGAA 1 cut(s) 1103
Bsp1286I GDGCHC 2 cut(s) 81, 155
Bsp143I GATC 7 cut(s) 265, 415, 598, 982, 1138, 1168, 1193
BspACI CCGC 3 cut(s) 18, 43, 1209
BspANI GGCC 1 cut(s) 937
BspCNI CTCAG 4 cut(s) 115, 940, 1001, 1150
BspLI GGNNCC 3 cut(s) 756, 781, 1140
BspPI GGATC 4 cut(s) 260, 593, 1133, 1146
BspT104I TTCGAA 1 cut(s) 1103
BspT107I GGYRCC 1 cut(s) 754
BspTNI GGTCTC 2 cut(s) 400, 630
BsrBI CCGCTC 2 cut(s) 18, 45
BsrDI GCAATG 3 cut(s) 293, 346, 1018
BsrI ACTGG 3 cut(s) 196, 637, 1244
BssMI GATC 7 cut(s) 265, 415, 598, 982, 1138, 1168, 1193
BssNAI GTATAC 1 cut(s) 624
BssNI GRCGYC 1 cut(s) 177
Bst1107I GTATAC 1 cut(s) 624
Bst4CI ACNGT 7 cut(s) 187, 300, 466, 566, 1032, 1265, 1326
Bst6I CTCTTC 2 cut(s) 603, 1272
BstACI GRCGYC 1 cut(s) 177
BstBAI YACGTR 1 cut(s) 54
BstBI TTCGAA 1 cut(s) 1103
BstC8I GCNNGC 5 cut(s) 43, 64, 81, 108, 432
BstDEI CTNAG 7 cut(s) 102, 218, 397, 536, 948, 988, 1158
BstF5I GGATG 6 cut(s) 711, 898, 952, 1089, 1228, 1279
BstH2I RGCGCY 1 cut(s) 1020
BstHHI GCGC 1 cut(s) 1019
BstKTI GATC 7 cut(s) 268, 418, 601, 985, 1141, 1171, 1196
BstMAI GTCTC 3 cut(s) 372, 400, 630
BstMBI GATC 7 cut(s) 265, 415, 598, 982, 1138, 1168, 1193
BstMWI GCNNNNNNNGC 3 cut(s) 63, 89, 112
BstV1I GCAGC 4 cut(s) 405, 515, 822, 881
BstV2I GAAGAC 2 cut(s) 38, 1263
BstX2I RGATCY 3 cut(s) 415, 598, 1138
BstYI RGATCY 3 cut(s) 415, 598, 1138
BstZ17I GTATAC 1 cut(s) 624
Bsu36I CCTNAGG 1 cut(s) 1158
BsuRI GGCC 1 cut(s) 937
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 6 cut(s) 711, 898, 952, 1089, 1228, 1279
BtsI GCAGTG 1 cut(s) 575
BtsIMutI CAGTG 7 cut(s) 16, 305, 313, 571, 575, 630, 1270
Cac8I GCNNGC 5 cut(s) 43, 64, 81, 108, 432
CfoI GCGC 1 cut(s) 1019
Cfr13I GGNCC 1 cut(s) 780
Csp6I GTAC 1 cut(s) 809
CviAII CATG 3 cut(s) 820, 839, 899
CviQI GTAC 1 cut(s) 809
DdeI CTNAG 7 cut(s) 102, 218, 397, 536, 948, 988, 1158
DpnI GATC 7 cut(s) 267, 417, 600, 984, 1140, 1170, 1195
DpnII GATC 7 cut(s) 265, 415, 598, 982, 1138, 1168, 1193
Eam1104I CTCTTC 2 cut(s) 603, 1272
EarI CTCTTC 2 cut(s) 603, 1272
Ecl136II GAGCTC 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 2 cut(s) 400, 630
Eco47I GGWCC 1 cut(s) 780
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 5 cut(s) 50, 289, 437, 627, 1041
Eco72I CACGTG 1 cut(s) 54
Eco81I CCTNAGG 1 cut(s) 1158
Eco88I CYCGRG 1 cut(s) 411
EcoICRI GAGCTC 1 cut(s) 79
EcoT22I ATGCAT 1 cut(s) 277
EcoT38I GRGCYC 1 cut(s) 81
FaeI CATG 3 cut(s) 823, 842, 902
FatI CATG 3 cut(s) 819, 838, 898
FauI CCCGC 1 cut(s) 50
FauNDI CATATG 1 cut(s) 1173
FbaI TGATCA 1 cut(s) 1168
FblI GTMKAC 1 cut(s) 623
Fnu4HI GCNGC 5 cut(s) 19, 394, 504, 836, 870
FokI GGATG 6 cut(s) 698, 905, 939, 1096, 1215, 1286
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 5 cut(s) 19, 394, 504, 836, 870
FspBI CTAG 4 cut(s) 770, 824, 1185, 1233
GlaI GCGC 1 cut(s) 1018
GluI GCNGC 5 cut(s) 19, 394, 504, 836, 870
GsaI CCCAGC 1 cut(s) 1122
GsuI CTGGAG 1 cut(s) 654
HaeII RGCGCY 1 cut(s) 1020
HaeIII GGCC 1 cut(s) 937
HhaI GCGC 1 cut(s) 1019
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 3 cut(s) 823, 842, 902
Hin6I GCGC 1 cut(s) 1017
HinP1I GCGC 1 cut(s) 1017
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 2 cut(s) 569, 1105
Hpy166II GTNNAC 3 cut(s) 624, 783, 1091
Hpy188I TCNGA 7 cut(s) 30, 420, 499, 913, 949, 961, 982
Hpy188III TCNNGA 6 cut(s) 269, 371, 455, 770, 890, 990
Hpy8I GTNNAC 3 cut(s) 624, 783, 1091
Hpy99I CGWCG 1 cut(s) 924
HpyAV CCTTC 6 cut(s) 46, 108, 504, 658, 667, 1016
HpyCH4III ACNGT 7 cut(s) 187, 300, 466, 566, 1032, 1265, 1326
HpyCH4IV ACGT 3 cut(s) 53, 133, 177
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 89, 112
HpyF3I CTNAG 7 cut(s) 102, 218, 397, 536, 948, 988, 1158
HpySE526I ACGT 3 cut(s) 53, 133, 177
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 3 cut(s) 823, 842, 902
HspAI GCGC 1 cut(s) 1017
Ksp22I TGATCA 1 cut(s) 1168
Kzo9I GATC 7 cut(s) 265, 415, 598, 982, 1138, 1168, 1193
LmnI GCTCC 1 cut(s) 50
Lsp1109I GCAGC 4 cut(s) 405, 515, 822, 881
LweI GCATC 8 cut(s) 75, 262, 284, 490, 787, 961, 1121, 1237
MaeI CTAG 4 cut(s) 770, 824, 1185, 1233
MaeII ACGT 3 cut(s) 53, 133, 177
MaeIII GTNAC 5 cut(s) 187, 573, 649, 828, 1235
MalI GATC 7 cut(s) 267, 417, 600, 984, 1140, 1170, 1195
MbiI CCGCTC 2 cut(s) 18, 45
MboI GATC 7 cut(s) 265, 415, 598, 982, 1138, 1168, 1193
MboII GAAGA 5 cut(s) 43, 620, 1190, 1268, 1289
MflI RGATCY 3 cut(s) 415, 598, 1138
MhlI GDGCHC 2 cut(s) 81, 155
MnlI CCTC 7 cut(s) 68, 512, 688, 870, 1019, 1153, 1264
Mph1103I ATGCAT 1 cut(s) 277
MroXI GAANNNNTTC 2 cut(s) 35, 677
MseI TTAA 2 cut(s) 159, 1203
MslI CAYNNNNRTG 2 cut(s) 647, 824
MspA1I CMGCKG 2 cut(s) 835, 1209
Mva1269I GAATGC 2 cut(s) 1060, 1293
MwoI GCNNNNNNNGC 3 cut(s) 63, 89, 112
NdeI CATATG 1 cut(s) 1173
NdeII GATC 7 cut(s) 265, 415, 598, 982, 1138, 1168, 1193
NlaIII CATG 3 cut(s) 823, 842, 902
NlaIV GGNNCC 3 cut(s) 756, 781, 1140
NmuCI GTSAC 3 cut(s) 573, 649, 828
NsiI ATGCAT 1 cut(s) 277
NspV TTCGAA 1 cut(s) 1103
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 2 cut(s) 1060, 1293
PdmI GAANNNNTTC 2 cut(s) 35, 677
PfeI GAWTC 2 cut(s) 569, 1105
PflMI CCANNNNNTGG 2 cut(s) 764, 975
PkrI GCNGC 5 cut(s) 20, 395, 505, 837, 871
PmaCI CACGTG 1 cut(s) 54
PmlI CACGTG 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 54
Psp124BI GAGCTC 1 cut(s) 81
PspCI CACGTG 1 cut(s) 54
PspFI CCCAGC 1 cut(s) 1118
PspN4I GGNNCC 3 cut(s) 756, 781, 1140
PspPI GGNCC 1 cut(s) 780
PsrI GAACNNNNNNTAC 2 cut(s) 339, 371
PsuI RGATCY 3 cut(s) 415, 598, 1138
PvuII CAGCTG 1 cut(s) 835
RsaI GTAC 1 cut(s) 810
RsaNI GTAC 1 cut(s) 809
RseI CAYNNNNRTG 2 cut(s) 647, 824
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 2 cut(s) 159, 1203
SatI GCNGC 5 cut(s) 19, 394, 504, 836, 870
Sau3AI GATC 7 cut(s) 265, 415, 598, 982, 1138, 1168, 1193
Sau96I GGNCC 1 cut(s) 780
SduI GDGCHC 2 cut(s) 81, 155
SfaNI GCATC 8 cut(s) 75, 262, 284, 490, 787, 961, 1121, 1237
SfuI TTCGAA 1 cut(s) 1103
SinI GGWCC 1 cut(s) 780
SmiMI CAYNNNNRTG 2 cut(s) 647, 824
SsiI CCGC 3 cut(s) 18, 43, 1209
SspMI CTAG 4 cut(s) 770, 824, 1185, 1233
SstI GAGCTC 1 cut(s) 81
TaaI ACNGT 7 cut(s) 187, 300, 466, 566, 1032, 1265, 1326
TaiI ACGT 3 cut(s) 56, 136, 180
TaqI TCGA 2 cut(s) 149, 1103
TatI WGTACW 1 cut(s) 808
TauI GCSGC 1 cut(s) 21
TfiI GAWTC 2 cut(s) 569, 1105
Tru1I TTAA 2 cut(s) 159, 1203
Tru9I TTAA 2 cut(s) 159, 1203
TscAI CASTG 7 cut(s) 16, 305, 313, 571, 582, 637, 1270
TseFI GTSAC 3 cut(s) 573, 649, 828
TseI GCWGC 4 cut(s) 393, 503, 835, 869
Tsp45I GTSAC 3 cut(s) 573, 649, 828
TspDTI ATGAA 4 cut(s) 329, 855, 1269, 1290
TspRI CASTG 7 cut(s) 16, 305, 313, 571, 582, 637, 1270
Van91I CCANNNNNTGG 2 cut(s) 764, 975
VpaK11BI GGWCC 1 cut(s) 780
XapI RAATTY 4 cut(s) 383, 790, 972, 1048
XbaI TCTAGA 1 cut(s) 769
XmiI GTMKAC 1 cut(s) 623
XmnI GAANNNNTTC 2 cut(s) 35, 677
XspI CTAG 4 cut(s) 770, 824, 1185, 1233
ZraI GACGTC 1 cut(s) 178
Zsp2I ATGCAT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.