pycom04g18120

Belongs to the peptidase M24B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011079
Physical Location & Seq
Forward (+)
20266378 .. 20271084
4707 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2031 bp
ATGCACTCACTGTCATCACAGGCAGTGCGCCCTCTCTCCTTGCCCTTCGCCCGCTCTCACTACTTCCGCCGCTTCCTTCCCCTCTCGCTCCCCATTTTCCACAATTCCAAGTCCCACCCCAAAATTTTCACTATTACTCCAAAAGCTCCGGTCATGACGATCCGAAATTGCTCCTCCATCTCTGCCAAGCCTTCGTCGGAGCTCCGAAACAAGCGATCCAGTTCAGATTCTTCCGACGAGAAGCTACGCGCCCTCCGCCAGCTCTTCTCGAAGCCCGGCGTCGGCATCGACGCCTATATCATTCCTTCTCAGGATGCTCACCAGAGTGAGTTCATTGCTGAATGTTATATGAGGAGGACCTTTATATCGGGGTTCACAGGAAGTGCTGGAACTGCTGTCGTCACAAAGGATAAAGCTGCTCTTTGGACAGATGGGCGCTACTTTCTTCAGGCTGAAAAGCAGCTGAGCTCTAGTTGGATTCTCATGCGGGCAGGTTCTCTAGGAGTTCCTACGACTATTGAATGGTTGAATAATGTTTTGGCTCCTGGTGGGAGAGTTGGCATCGATCCTTTTCTGTTTTCTTCTGAGGCTGCAGAAGAATTGAAACAAGGTATTGCAAAGAAGAACCATGAGTTAGTTTTCCTTTATGATAAAAATCTTGTGGATGAAGTATGGAATGAATCAAGACCGAAGCCTCCAAACAAACCAGTGAGAGTACATGATCTCAAGTATGCTGGTGTAGATGTAGCATCAAAGTTGTTGTCTTTGAGGTCGGAACTTGCTGATGCTGGTTCATCTGCGATAATTATATCTATGCTCGATGAAGTTGCCTGGCTATTGAACTTGAGAGGAAGTGATGTTCCGCATTCACCCGTTATGTATGCATATCTGATTGTAGAGATTGATAAAGCAAAATTATTTATAGATACTTCTAAAGTGAACACAGAGGTGATGGATCACTTGAAGAAGGCAGGCATTGAATTGTTGCCATATGACTCCATTCTTTCTGAAGTAGAAAGATATTATGAGAGCCTTGAAAGTAAAAGAAAAGGTAAGAGTGACGACTCAAATGGTTGGTCCGGAGGACCGACTGGAATTTATAGAGTGTCCCCTGTTTCTTTGGCAAAGGCAGTTAAAAATGATTCTGAGTTGGAAGGCATGCAGAGTTGTCATTTAAAAGATGCTGCTGCTCTAGTGCAGTTTTGGGTATGGATGGAGGAGAAAATTAATGAGAACGTGAAATTGACTGAGGTAGAAGTTGCCGAAAAACTTCTTGAATTTCGTTCGGCGCAGTCTGGTTTCCTAGATACAAGCTTTGACACCATAAGCGGTTCTGGTGCAAATGGTGCTATCATACATTACAGAGCAGAACCAGATAGTTGTAGTGTTGTGGATGACAAGAAACTCTTCCTATTGGATAGCGGAGGTCAATATGTTGATGGAACAACTGACATAACTAGGACTGTACATTTTGGTGAACCTACCTCGCGACAAAAAGAATGCTTTACCCGAGTTTTGCAGGGTCATATAGCTCTTGATGAGGCAGTATTCCCTGAAAACATGCCTGGTTTTGTGTTAGATGCATTTGCTCGATCTTCCCTTTGGAAGATAGGACTGGATTATCGACATGGAACTGGGCATGGTGTGGGAGCTGCATTAAATGTTCATGAGGGACCTCAAGGTATTAGCTTTCGATTTGGAAATATGACTCCATTACAGAAGGGCATGATTGTTAGCAATGAACCTGGCTATTATGAAGACCATGCCTTTGGCATTCGGATTGAGAATCTCCTAGTTGTGAAAGAAGTCGACACACCAAATCGTTTTGGAGGAATTAGCTACCTGGGGTTTGAAAAACTCACATTTGTCCCGATTCAGGCTAAAATGATCGACTTATCTTTGCTTTCTGCTGCGGAGTTTGATTGGCTTAATGATTACCATTCACAAGTTTGGGAGAAGGTCTCCCCTCTTGTGGAGGGTTCTGCTCGTCAATGGCTTTGGGACAACACAAGGCCGCTCGTGAAGCAGTGA

Protein Analysis

677

Amino Acids

75.23

Weight (kDa)

6.17

Isoelectric Point (pI)

48.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Creatinase_N PF01321 84 - 208 2.7e-12 Creatinase/Prolidase N-terminal domain
Creatinase_N_2 PF16189 229 - 383 1.3e-42 Creatinase/Prolidase N-terminal domain
Peptidase_M24 PF00557 386 - 602 7.8e-50 Metallopeptidase family M24
Peptidase_M24_C PF16188 614 - 673 6.3e-25 C-terminal region of peptidase_M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 482
AccBSI CCGCTC 2 cut(s) 54, 2017
AccI GTMKAC 1 cut(s) 1809
AccII CGCG 2 cut(s) 249, 1489
AccIII TCCGGA 1 cut(s) 1079
AclWI GGATC 4 cut(s) 154, 210, 560, 963
AcsI RAATTY 3 cut(s) 123, 1095, 1277
AcuI CTGAAG 2 cut(s) 431, 1029
AcyI GRCGYC 2 cut(s) 279, 291
AfaI GTAC 2 cut(s) 717, 1467
AfiI CCNNNNNNNGG 3 cut(s) 281, 1876, 1972
AgsI TTSAA 9 cut(s) 521, 529, 604, 841, 964, 980, 1037, 1277, 1853
AjnI CCWGG 5 cut(s) 544, 830, 1564, 1744, 1842
AjuI GAANNNNNNNTTGG 2 cut(s) 521, 553
AleI CACNNNNGTG 2 cut(s) 324, 947
AloI GAACNNNNNNTCC 2 cut(s) 843, 875
Alw21I GWGCWC 2 cut(s) 204, 470
Alw26I GTCTC 1 cut(s) 1966
AlwI GGATC 4 cut(s) 154, 210, 560, 963
Ama87I CYCGRG 1 cut(s) 1509
Aor13HI TCCGGA 1 cut(s) 1079
AoxI GGCC 1 cut(s) 2012
ApeKI GCWGC 7 cut(s) 416, 460, 590, 1184, 1187, 1652, 1910
ApoI RAATTY 3 cut(s) 123, 1095, 1277
ArsI GACNNNNNNTTYG 6 cut(s) 746, 778, 1061, 1093, 1751, 1783
AseI ATTAAT 1 cut(s) 1227
Asp700I GAANNNNTTC 2 cut(s) 1269, 1406
AspLEI GCGC 4 cut(s) 30, 251, 438, 1291
AspS9I GGNCC 4 cut(s) 357, 1077, 1085, 1673
AsuC2I CCSGG 1 cut(s) 276
AsuHPI GGTGA 4 cut(s) 311, 861, 961, 1487
AvaI CYCGRG 1 cut(s) 1509
AvaII GGWCC 4 cut(s) 357, 1077, 1085, 1673
BanII GRGCYC 2 cut(s) 204, 470
BauI CACGAG 1 cut(s) 2018
BbsI GAAGAC 1 cut(s) 1764
Bbv12I GWGCWC 2 cut(s) 204, 470
BbvI GCAGC 7 cut(s) 403, 472, 577, 1171, 1174, 1639, 1897
BccI CCATC 5 cut(s) 185, 425, 946, 1207, 1433
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 5 cut(s) 546, 832, 1566, 1746, 1844
BcnI CCSGG 1 cut(s) 276
BcoDI GTCTC 1 cut(s) 1966
BfaI CTAG 6 cut(s) 471, 500, 1193, 1304, 1458, 1793
BfmI CTRYAG 1 cut(s) 591
BfoI RGCGCY 1 cut(s) 439
BfuAI ACCTGC 1 cut(s) 482
BisI GCNGC 9 cut(s) 70, 417, 461, 591, 1185, 1188, 1653, 1911, 2015
BlpI GCTNAGC 1 cut(s) 464
BlsI GCNGC 9 cut(s) 71, 418, 462, 592, 1186, 1189, 1654, 1912, 2016
Bme1390I CCNGG 6 cut(s) 276, 546, 832, 1566, 1746, 1844
Bme18I GGWCC 4 cut(s) 357, 1077, 1085, 1673
BmeT110I CYCGRG 1 cut(s) 1509
BmgT120I GGNCC 4 cut(s) 357, 1077, 1085, 1673
BmiI GGNNCC 2 cut(s) 543, 1674
BmrFI CCNGG 6 cut(s) 276, 546, 832, 1566, 1746, 1844
BmrI ACTGGG 1 cut(s) 1644
BmsI GCATC 7 cut(s) 294, 304, 570, 758, 775, 1171, 1570
BmuI ACTGGG 1 cut(s) 1644
BpiI GAAGAC 1 cut(s) 1764
BplI GAGNNNNNCTC 2 cut(s) 1946, 1978
Bpu1102I GCTNAGC 1 cut(s) 464
BpuEI CTTGAG 3 cut(s) 710, 865, 1662
BpuMI CCSGG 1 cut(s) 276
Bsa29I ATCGAT 1 cut(s) 564
BsaBI GATNNNNATC 1 cut(s) 654
BsaHI GRCGYC 2 cut(s) 279, 291
BsaI GGTCTC 1 cut(s) 1966
BsaJI CCNNGG 1 cut(s) 1843
BsaWI WCCGGW 2 cut(s) 148, 1079
BsaXI ACNNNNNCTCC 4 cut(s) 121, 151, 237, 267
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 1876, 1972
Bse1I ACTGG 5 cut(s) 219, 707, 1096, 1620, 1639
Bse3DI GCAATG 2 cut(s) 333, 1744
Bse8I GATNNNNATC 1 cut(s) 654
BseAI TCCGGA 1 cut(s) 1079
BseBI CCWGG 5 cut(s) 546, 832, 1566, 1746, 1844
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 1 cut(s) 1843
BseGI GGATG 4 cut(s) 319, 670, 1218, 1399
BseJI GATNNNNATC 1 cut(s) 654
BseLI CCNNNNNNNGG 3 cut(s) 281, 1876, 1972
BseMI GCAATG 2 cut(s) 333, 1744
BseMII CTCAG 5 cut(s) 323, 455, 576, 1137, 1239
BseNI ACTGG 5 cut(s) 219, 707, 1096, 1620, 1639
BseRI GAGGAG 3 cut(s) 163, 367, 1232
BseXI GCAGC 7 cut(s) 403, 472, 577, 1171, 1174, 1639, 1897
BsgI GTGCAG 1 cut(s) 1217
Bsh1236I CGCG 2 cut(s) 249, 1489
BshFI GGCC 1 cut(s) 2014
BshVI ATCGAT 1 cut(s) 564
BsiHKAI GWGCWC 2 cut(s) 204, 470
BsiHKCI CYCGRG 1 cut(s) 1509
BsiSI CCGG 3 cut(s) 149, 276, 1080
BslFI GGGAC 5 cut(s) 97, 1093, 1686, 1853, 2015
BslI CCNNNNNNNGG 3 cut(s) 281, 1876, 1972
BsmAI GTCTC 1 cut(s) 1966
BsmFI GGGAC 5 cut(s) 97, 1093, 1686, 1853, 2015
BsmI GAATGC 3 cut(s) 865, 1505, 1773
BsnI GGCC 1 cut(s) 2014
Bso31I GGTCTC 1 cut(s) 1966
BsoBI CYCGRG 1 cut(s) 1509
Bsp1286I GDGCHC 2 cut(s) 204, 470
Bsp13I TCCGGA 1 cut(s) 1079
Bsp1407I TGTACA 1 cut(s) 1465
Bsp143I GATC 7 cut(s) 159, 215, 565, 721, 955, 1592, 1887
Bsp1720I GCTNAGC 1 cut(s) 464
Bsp68I TCGCGA 1 cut(s) 1489
BspANI GGCC 1 cut(s) 2014
BspCNI CTCAG 5 cut(s) 322, 456, 577, 1138, 1240
BspDI ATCGAT 1 cut(s) 564
BspEI TCCGGA 1 cut(s) 1079
BspFNI CGCG 2 cut(s) 249, 1489
BspHI TCATGA 2 cut(s) 153, 1666
BspLI GGNNCC 2 cut(s) 543, 1674
BspMAI CTGCAG 1 cut(s) 595
BspMI ACCTGC 1 cut(s) 482
BspPI GGATC 4 cut(s) 154, 210, 560, 963
BspQI GCTCTTC 1 cut(s) 269
BspTNI GGTCTC 1 cut(s) 1966
BsrBI CCGCTC 2 cut(s) 54, 2017
BsrDI GCAATG 2 cut(s) 333, 1744
BsrGI TGTACA 1 cut(s) 1465
BsrI ACTGG 5 cut(s) 219, 707, 1096, 1620, 1639
BssECI CCNNGG 1 cut(s) 1843
BssMI GATC 7 cut(s) 159, 215, 565, 721, 955, 1592, 1887
BssNI GRCGYC 2 cut(s) 279, 291
BssSI CACGAG 1 cut(s) 2018
Bst2BI CACGAG 1 cut(s) 2018
Bst2UI CCWGG 5 cut(s) 546, 832, 1566, 1746, 1844
Bst4CI ACNGT 2 cut(s) 12, 1465
Bst6I CTCTTC 2 cut(s) 269, 1412
BstACI GRCGYC 2 cut(s) 279, 291
BstAPI GCANNNNNTGC 1 cut(s) 1346
BstAUI TGTACA 1 cut(s) 1465
BstC8I GCNNGC 5 cut(s) 52, 260, 489, 973, 1160
BstDEI CTNAG 5 cut(s) 309, 464, 585, 1146, 1248
BstF5I GGATG 4 cut(s) 319, 670, 1218, 1399
BstFNI CGCG 2 cut(s) 249, 1489
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 4 cut(s) 30, 251, 438, 1291
BstKTI GATC 7 cut(s) 162, 218, 568, 724, 958, 1595, 1890
BstMAI GTCTC 1 cut(s) 1966
BstMBI GATC 7 cut(s) 159, 215, 565, 721, 955, 1592, 1887
BstMWI GCNNNNNNNGC 4 cut(s) 255, 392, 1346, 2023
BstNI CCWGG 5 cut(s) 546, 832, 1566, 1746, 1844
BstNSI RCATGY 2 cut(s) 1162, 1564
BstSCI CCNGG 6 cut(s) 274, 544, 830, 1564, 1744, 1842
BstSFI CTRYAG 1 cut(s) 591
BstUI CGCG 2 cut(s) 249, 1489
BstV1I GCAGC 7 cut(s) 403, 472, 577, 1171, 1174, 1639, 1897
BstV2I GAAGAC 1 cut(s) 1764
BstXI CCANNNNNNTGG 1 cut(s) 1769
Bsu15I ATCGAT 1 cut(s) 564
BsuRI GGCC 1 cut(s) 2014
BsuTUI ATCGAT 1 cut(s) 564
BtsCI GGATG 4 cut(s) 319, 670, 1218, 1399
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 3 cut(s) 8, 30, 714
BtuMI TCGCGA 1 cut(s) 1489
BveI ACCTGC 1 cut(s) 482
Cac8I GCNNGC 5 cut(s) 52, 260, 489, 973, 1160
CciI TCATGA 2 cut(s) 153, 1666
CfoI GCGC 4 cut(s) 30, 251, 438, 1291
Cfr13I GGNCC 4 cut(s) 357, 1077, 1085, 1673
ClaI ATCGAT 1 cut(s) 564
CseI GACGC 2 cut(s) 268, 299
Csp6I GTAC 2 cut(s) 716, 1466
CviQI GTAC 2 cut(s) 716, 1466
DdeI CTNAG 5 cut(s) 309, 464, 585, 1146, 1248
DpnI GATC 7 cut(s) 161, 217, 567, 723, 957, 1594, 1889
DpnII GATC 7 cut(s) 159, 215, 565, 721, 955, 1592, 1887
DraI TTTAAA 1 cut(s) 1176
Eam1104I CTCTTC 2 cut(s) 269, 1412
EarI CTCTTC 2 cut(s) 269, 1412
EciI GGCGGA 2 cut(s) 56, 245
Ecl136II GAGCTC 2 cut(s) 202, 468
Eco24I GRGCYC 2 cut(s) 204, 470
Eco31I GGTCTC 1 cut(s) 1966
Eco47I GGWCC 4 cut(s) 357, 1077, 1085, 1673
Eco53kI GAGCTC 2 cut(s) 202, 468
Eco57I CTGAAG 2 cut(s) 431, 1029
Eco88I CYCGRG 1 cut(s) 1509
EcoICRI GAGCTC 2 cut(s) 202, 468
EcoO109I RGGNCCY 2 cut(s) 357, 1673
EcoRII CCWGG 5 cut(s) 544, 830, 1564, 1744, 1842
EcoT22I ATGCAT 2 cut(s) 886, 1585
EcoT38I GRGCYC 2 cut(s) 204, 470
FalI AAGNNNNNCTT 4 cut(s) 405, 437, 1391, 1423
FaqI GGGAC 5 cut(s) 97, 1093, 1686, 1853, 2015
FauI CCCGC 2 cut(s) 59, 480
FauNDI CATATG 1 cut(s) 991
FblI GTMKAC 1 cut(s) 1809
Fnu4HI GCNGC 9 cut(s) 70, 417, 461, 591, 1185, 1188, 1653, 1911, 2015
FokI GGATG 4 cut(s) 326, 677, 1225, 1406
FriOI GRGCYC 2 cut(s) 204, 470
Fsp4HI GCNGC 9 cut(s) 70, 417, 461, 591, 1185, 1188, 1653, 1911, 2015
FspBI CTAG 6 cut(s) 471, 500, 1193, 1304, 1458, 1793
GlaI GCGC 4 cut(s) 29, 250, 437, 1290
GluI GCNGC 9 cut(s) 70, 417, 461, 591, 1185, 1188, 1653, 1911, 2015
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 1 cut(s) 2014
HapII CCGG 3 cut(s) 149, 276, 1080
HgaI GACGC 2 cut(s) 268, 299
HhaI GCGC 4 cut(s) 30, 251, 438, 1291
Hin1I GRCGYC 2 cut(s) 279, 291
Hin6I GCGC 4 cut(s) 28, 249, 436, 1289
HinP1I GCGC 4 cut(s) 28, 249, 436, 1289
HincII GTYRAC 1 cut(s) 1810
HindII GTYRAC 1 cut(s) 1810
HindIII AAGCTT 1 cut(s) 1312
HinfI GANTC 9 cut(s) 227, 478, 680, 995, 1064, 1142, 1708, 1786, 1873
HpaII CCGG 3 cut(s) 149, 276, 1080
HphI GGTGA 4 cut(s) 311, 861, 961, 1487
Hpy166II GTNNAC 4 cut(s) 375, 940, 1478, 1810
Hpy8I GTNNAC 4 cut(s) 375, 940, 1478, 1810
Hpy99I CGWCG 4 cut(s) 199, 239, 284, 293
HpyAV CCTTC 8 cut(s) 55, 86, 201, 315, 961, 1148, 1714, 1951
HpyCH4III ACNGT 2 cut(s) 12, 1465
HpyCH4IV ACGT 1 cut(s) 1236
HpyF10VI GCNNNNNNNGC 4 cut(s) 255, 392, 1346, 2023
HpyF3I CTNAG 5 cut(s) 309, 464, 585, 1146, 1248
HpySE526I ACGT 1 cut(s) 1236
Hsp92I GRCGYC 2 cut(s) 279, 291
HspAI GCGC 4 cut(s) 28, 249, 436, 1289
Kpn2I TCCGGA 1 cut(s) 1079
Kzo9I GATC 7 cut(s) 159, 215, 565, 721, 955, 1592, 1887
LguI GCTCTTC 1 cut(s) 269
LmnI GCTCC 7 cut(s) 93, 151, 176, 199, 207, 547, 1649
Lsp1109I GCAGC 7 cut(s) 403, 472, 577, 1171, 1174, 1639, 1897
LweI GCATC 7 cut(s) 294, 304, 570, 758, 775, 1171, 1570
MaeI CTAG 6 cut(s) 471, 500, 1193, 1304, 1458, 1793
MaeII ACGT 1 cut(s) 1236
MaeIII GTNAC 2 cut(s) 400, 1058
MalI GATC 7 cut(s) 161, 217, 567, 723, 957, 1594, 1889
MbiI CCGCTC 2 cut(s) 54, 2017
MboI GATC 7 cut(s) 159, 215, 565, 721, 955, 1592, 1887
MhlI GDGCHC 2 cut(s) 204, 470
MlyI GAGTC 3 cut(s) 989, 1058, 1702
MmeI TCCRAC 5 cut(s) 177, 258, 455, 753, 1131
Mph1103I ATGCAT 2 cut(s) 886, 1585
MroI TCCGGA 1 cut(s) 1079
MroXI GAANNNNTTC 2 cut(s) 1269, 1406
MseI TTAA 5 cut(s) 1134, 1175, 1227, 1658, 1929
MslI CAYNNNNRTG 3 cut(s) 324, 947, 1473
MspA1I CMGCKG 1 cut(s) 463
MspI CCGG 3 cut(s) 149, 276, 1080
MspR9I CCNGG 6 cut(s) 276, 546, 832, 1566, 1746, 1844
Mva1269I GAATGC 3 cut(s) 865, 1505, 1773
MvaI CCWGG 5 cut(s) 546, 832, 1566, 1746, 1844
MvnI CGCG 2 cut(s) 249, 1489
MwoI GCNNNNNNNGC 4 cut(s) 255, 392, 1346, 2023
NciI CCSGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 991
NdeII GATC 7 cut(s) 159, 215, 565, 721, 955, 1592, 1887
NlaIV GGNNCC 2 cut(s) 543, 1674
NmuCI GTSAC 2 cut(s) 400, 1058
NruI TCGCGA 1 cut(s) 1489
NsiI ATGCAT 2 cut(s) 886, 1585
NspI RCATGY 2 cut(s) 1162, 1564
OliI CACNNNNGTG 2 cut(s) 324, 947
PaeI GCATGC 1 cut(s) 1162
PagI TCATGA 2 cut(s) 153, 1666
PciSI GCTCTTC 1 cut(s) 269
PctI GAATGC 3 cut(s) 865, 1505, 1773
PdmI GAANNNNTTC 2 cut(s) 1269, 1406
PfeI GAWTC 6 cut(s) 227, 478, 680, 1142, 1786, 1873
PkrI GCNGC 9 cut(s) 71, 418, 462, 592, 1186, 1189, 1654, 1912, 2016
PleI GAGTC 3 cut(s) 989, 1058, 1702
PpsI GAGTC 3 cut(s) 989, 1058, 1702
PpuMI RGGWCCY 2 cut(s) 357, 1673
PshBI ATTAAT 1 cut(s) 1227
Psp124BI GAGCTC 2 cut(s) 204, 470
Psp5II RGGWCCY 2 cut(s) 357, 1673
Psp6I CCWGG 5 cut(s) 544, 830, 1564, 1744, 1842
PspGI CCWGG 5 cut(s) 544, 830, 1564, 1744, 1842
PspN4I GGNNCC 2 cut(s) 543, 1674
PspPI GGNCC 4 cut(s) 357, 1077, 1085, 1673
PspPPI RGGWCCY 2 cut(s) 357, 1673
PstI CTGCAG 1 cut(s) 595
PvuII CAGCTG 1 cut(s) 463
RruI TCGCGA 1 cut(s) 1489
RsaI GTAC 2 cut(s) 717, 1467
RsaNI GTAC 2 cut(s) 716, 1466
RseI CAYNNNNRTG 3 cut(s) 324, 947, 1473
SacI GAGCTC 2 cut(s) 204, 470
SalI GTCGAC 1 cut(s) 1808
SapI GCTCTTC 1 cut(s) 269
SaqAI TTAA 5 cut(s) 1134, 1175, 1227, 1658, 1929
SatI GCNGC 9 cut(s) 70, 417, 461, 591, 1185, 1188, 1653, 1911, 2015
Sau3AI GATC 7 cut(s) 159, 215, 565, 721, 955, 1592, 1887
Sau96I GGNCC 4 cut(s) 357, 1077, 1085, 1673
SchI GAGTC 3 cut(s) 989, 1058, 1702
ScrFI CCNGG 6 cut(s) 276, 546, 832, 1566, 1746, 1844
SduI GDGCHC 2 cut(s) 204, 470
SfaNI GCATC 7 cut(s) 294, 304, 570, 758, 775, 1171, 1570
SfcI CTRYAG 1 cut(s) 591
SinI GGWCC 4 cut(s) 357, 1077, 1085, 1673
SmiMI CAYNNNNRTG 3 cut(s) 324, 947, 1473
SmlI CTYRAG 3 cut(s) 725, 844, 1677
SmoI CTYRAG 3 cut(s) 725, 844, 1677
SphI GCATGC 1 cut(s) 1162
SspMI CTAG 6 cut(s) 471, 500, 1193, 1304, 1458, 1793
SstI GAGCTC 2 cut(s) 204, 470
StyD4I CCNGG 6 cut(s) 274, 544, 830, 1564, 1744, 1842
TaaI ACNGT 2 cut(s) 12, 1465
TaiI ACGT 1 cut(s) 1239
TaqI TCGA 9 cut(s) 269, 288, 564, 819, 1591, 1624, 1693, 1809, 1890
TaqII GACCGA 2 cut(s) 703, 1102
TatI WGTACW 2 cut(s) 715, 1465
TauI GCSGC 2 cut(s) 72, 2017
TfiI GAWTC 6 cut(s) 227, 478, 680, 1142, 1786, 1873
Tru1I TTAA 5 cut(s) 1134, 1175, 1227, 1658, 1929
Tru9I TTAA 5 cut(s) 1134, 1175, 1227, 1658, 1929
TscAI CASTG 3 cut(s) 15, 30, 714
TseFI GTSAC 2 cut(s) 400, 1058
TseI GCWGC 7 cut(s) 416, 460, 590, 1184, 1187, 1652, 1910
Tsp45I GTSAC 2 cut(s) 400, 1058
TspDTI ATGAA 8 cut(s) 322, 681, 693, 783, 837, 1655, 1755, 1770
TspRI CASTG 3 cut(s) 15, 30, 714
VpaK11BI GGWCC 4 cut(s) 357, 1077, 1085, 1673
VspI ATTAAT 1 cut(s) 1227
XapI RAATTY 3 cut(s) 123, 1095, 1277
XceI RCATGY 2 cut(s) 1162, 1564
XmiI GTMKAC 1 cut(s) 1809
XmnI GAANNNNTTC 2 cut(s) 1269, 1406
XspI CTAG 6 cut(s) 471, 500, 1193, 1304, 1458, 1793
Zsp2I ATGCAT 2 cut(s) 886, 1585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.